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1.
Zhongguo Zhen Jiu ; 40(10): 1133-5, 2020 Oct 12.
Artigo em Chinês | MEDLINE | ID: mdl-33068360

RESUMO

On the base of the records in Huangdi Neijing (Yellow Emperor 's Inner Classic) and relevant ancient literature, the anatomical structure of the belt vessel was revivified. The belt vessel covers the kidneys and inlays in the 14th vertebrae on the lumbar region. It joints qijie (qi street) at the groin and connects with the thoroughfare vessel, the conception vessel and the governor vessel, as well as the muscle region of foot-yangming meridian. Correspondingly, the related anatomic structure includes renal fascia and transversalis fascia. The transversalis fascia is the main part of the belt vessel structure. The superior lumbar triangle is the vulnerable spot of abdominal wall structure and also coincident with the localization of "3 cun away from the spinal column bilaterally". It is the optimal selection when stimulating the belt vessel. In late generations, "governing all of meridians" has been supplemented as the function of the belt vessel. The diaphragm extends to the transversalis fascia and renal fascia through the inferior diaphragmatic fascia and it is also the only structure that connects with the twelve meridians and five zang organs. Hence, modern acupuncture and moxibustion has actually transferred the structural center of the belt vessel from the transversalis fascia to the diaphragm.


Assuntos
Acupuntura , Fáscia/anatomia & histologia , Rim/anatomia & histologia , Vértebras Lombares/anatomia & histologia , Meridianos , Pontos de Acupuntura , Humanos , Moxibustão
2.
Am J Physiol Renal Physiol ; 319(6): F1027-F1036, 2020 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-33103446

RESUMO

Similar to other organs, renal lymphatics remove excess fluid, solutes, and macromolecules from the renal interstitium. Given the kidney's unique role in maintaining body fluid homeostasis, renal lymphatics may be critical in this process. However, little is known regarding the pathways involved in renal lymphatic vessel function, and there are no studies on the effects of drugs targeting impaired interstitial clearance, such as diuretics. Using pressure myography, we showed that renal lymphatic collecting vessels are sensitive to changes in transmural pressure and have an optimal range of effective pumping. In addition, they are responsive to vasoactive factors known to regulate tone in other lymphatic vessels including prostaglandin E2 and nitric oxide, and their spontaneous contractility requires Ca2+ and Cl-. We also demonstrated that Na+-K+-2Cl- cotransporter Nkcc1, but not Nkcc2, is expressed in extrarenal lymphatic vessels. Furosemide, a loop diuretic that inhibits Na+-K+-2Cl- cotransporters, induced a dose-dependent dilation in lymphatic vessels and decreased the magnitude and frequency of spontaneous contractions, thereby reducing the ability of these vessels to propel lymph. Ethacrynic acid, another loop diuretic, had no effect on vessel tone. These data represent a significant step forward in our understanding of the mechanisms underlying renal lymphatic vessel function and highlight potential off-target effects of furosemide that may exacerbate fluid accumulation in edema-forming conditions.


Assuntos
Rim/anatomia & histologia , Rim/fisiologia , Vasos Linfáticos/fisiologia , Animais , Cálcio/metabolismo , Cloretos/metabolismo , Furosemida/farmacologia , Vasos Linfáticos/efeitos dos fármacos , Masculino , NG-Nitroarginina Metil Éster , Óxido Nítrico/metabolismo , Pressão , Ratos , Ratos Sprague-Dawley , Inibidores de Simportadores de Cloreto de Sódio e Potássio/farmacologia
3.
Am J Physiol Renal Physiol ; 319(2): F245-F255, 2020 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-32567348

RESUMO

Ca2+ is an important second messenger that translates extracellular stimuli into intracellular responses. Although there has been significant progress in understanding Ca2+ dynamics in organs such as the brain, the nature of Ca2+ signals in the kidney is still poorly understood. Here, we show that by using a genetically expressed highly sensitive reporter (GCaMP6s), it is possible to perform imaging of Ca2+ signals at high resolution in the mouse kidney in vivo. Moreover, by applying machine learning-based automated analysis using a Ca2+-independent signal, quantitative data can be extracted in an unbiased manner. By projecting the resulting data onto the structure of the kidney, we show that different tubular segments display highly distinct spatiotemporal patterns of Ca2+ signals. Furthermore, we provide evidence that Ca2+ activity in the proximal tubule decreases with increasing distance from the glomerulus. Finally, we demonstrate that substantial changes in intracellular Ca2+ can be detected in proximal tubules in a cisplatin model of acute kidney injury, which can be linked to alterations in cell structure and transport function. In summary, we describe a powerful new tool to investigate how single cell behavior is integrated with whole organ structure and function and how it is altered in disease states relevant to humans.


Assuntos
Sinalização do Cálcio/fisiologia , Cálcio/metabolismo , Glomérulos Renais/metabolismo , Túbulos Renais Proximais/metabolismo , Lesão Renal Aguda/metabolismo , Animais , Humanos , Rim/anatomia & histologia , Rim/metabolismo , Túbulos Renais Proximais/anatomia & histologia , Camundongos
4.
Eur. j. anat ; 24(3): 171-191, mayo 2020. tab, graf, ilus
Artigo em Inglês | IBECS | ID: ibc-191467

RESUMO

The kidney plays a vital role in eliminating the toxic substances and drug metabolites from blood. Therefore, it is prone to hazards in case of abuse or overdose of drugs. The aim of the work was to clarify the impact of Moringa oleifera (MO) against the nephrotoxic effect of paracetamol in adult male albino rats. Twenty-eight adult male albino rats were used in this study and divided equally into four groups (G); G1 (control G), G2 (paracetamol-treated animals), G3 (paracetamol- plus high MO-treated G), G4 (high MO-treated rats). Kidney specimens were harvested after anesthetizing the animals, and then processed for histological and DNA comet analysis. Biochemical blood investiga-tions for kidney function were also performed through assessment of serum creatinine (Cr) and blood urea nitrogen (BUN). Administration of para-cetamol resulted in genotoxicity and marked bio-chemical and renal histological changes. It caused a significant increase in Cr and BUN levels and degeneration in both proximal and distal convolut-ed tubules with glomerular changes. Administration of MO with paracetamol led to a noticeable amelioration of previous mentioned changes. Moringa oleifera is suggested to be an effectivenephroprotection against paracetamol-induced damage. Further studies are recommended to con-firm the protective role of MO using large numbers of different animal models before investigating it in humans


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Assuntos
Animais , Masculino , Ratos , Acetaminofen/efeitos adversos , Envenenamento/veterinária , Rim/anatomia & histologia , Moringa oleifera/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Moringa oleifera/metabolismo , Rim/efeitos dos fármacos , Modelos Animais , Peso Corporal/efeitos dos fármacos , Fotomicrografia
5.
J Morphol ; 281(7): 725-736, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32374496

RESUMO

We studied the opisthonephric (mesonephric) kidneys of adult male and female Xenopus laevis using scanning electron microscopy (SEM) of vascular corrosion casts and light microscopy of paraplast embedded tissue sections. Both techniques displayed glomeruli from ventral to mid-dorsal regions of the kidneys with single glomeruli located dorsally close beneath the renal capsule. Glomeruli in general were fed by a single afferent arteriole and drained via a single thinner efferent arteriole into peritubular vessels. Light microscopy and SEM of vascular corrosion casts revealed sphincters at the origins of afferent arterioles, which arose closely, spaced from their parent renal arteries. The second source of renal blood supply via renal portal veins varied interindividually in branching patterns with vessels showing up to five branching orders before they became peritubular vessels. Main trunks and their first- and second-order branches revealed clear longish endothelial cell nuclei imprint patterns oriented parallel to the vessels longitudinal axis, a pattern characteristic for arteries. Peritubular vessels had irregular contours and were never seen as clear cylindrical structures. They ran rather parallel, anastomosed with neighbors and changed into renal venules and veins, which finally emptied into the ventrally located posterior caval vein. A third source of blood supply of the peritubular vessels by straight terminal portions of renal arteries (vasa recta) was not found.


Assuntos
Molde por Corrosão , Rim/irrigação sanguínea , Microscopia Eletrônica de Varredura , Microvasos/ultraestrutura , Xenopus laevis/fisiologia , Animais , Artérias/anatomia & histologia , Arteríolas/anatomia & histologia , Feminino , Imageamento Tridimensional , Rim/anatomia & histologia , Masculino , Microvasos/anatomia & histologia , Veias/anatomia & histologia
6.
Am J Forensic Med Pathol ; 41(2): 110-114, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32205487

RESUMO

Organ weights are routinely measured during autopsies as a crude screening tool to detect possible organ pathology. In several centers, inclusion of major organ weights indicates whether an autopsy report has achieved its standard of practice, which in turn should be subjected to an audit. Previous studies show statistical variation in organ weights across different populations. Malaysian pathologists have relied on Western data and crude subjective determination in the interpretation of normal organ weights. Hence, the need for a reference range as a guide for pathologists is acute. Organ weights from traumatic deaths between 2004 and 2017 were analyzed in the UKM Medical Centre. Statistical analysis was performed to form reference ranges for normal weights of the brain, heart, lung, liver, spleen, and kidneys. In addition, the data were compared between sexes, races, and body mass index values to determine whether organ weights were affected by these parameters. In this study, reference ranges for organ weights are presented for Malaysian adult men and women.


Assuntos
Medicina Legal , Tamanho do Órgão , Adulto , Índice de Massa Corporal , Encéfalo/anatomia & histologia , Feminino , Coração/anatomia & histologia , Humanos , Rim/anatomia & histologia , Fígado/anatomia & histologia , Pulmão/anatomia & histologia , Malásia , Masculino , Valores de Referência , Estudos Retrospectivos , Caracteres Sexuais , Baço/anatomia & histologia
7.
Female Pelvic Med Reconstr Surg ; 26(2): 111-115, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31990798

RESUMO

OBJECTIVE: Horseshoe kidney (HSK) is the most common renal fusion defect that can alter vascular and upper urinary tract anatomy. Anatomic variations in the presacral space can make surgical dissection very challenging. The aim of this study was to characterize presacral anatomy in women with HSK. METHODS: Large academic centers' database was queried to identify imaging studies in adult women with HSK. Available multiplanar computed tomography and magnetic resonance imaging images were reviewed, and relevant vascular and upper urinary tract anatomy was measured and compared with published normal values. Study population was compared with the normal controls using Student t test, χ test, or Fisher exact test as appropriate. RESULTS: One hundred seventy-eight women were identified initially, and 20 confirmed to have HSK on imaging. The mean ± SD age was 54.5 ± 16.9 years, and body mass index was 27.3 ± 7.5 kg/m. Women with HSK had a narrower angle of aortic bifurcation (39.1 ± 18.7 degrees vs 55.6 ± 4.5 degrees, P = 0.014); the right ureter was closer to midline (22.9 ± 8.8 mm vs 32.3 ± 1.2 mm, P < 0.001) when compared with normal controls anatomy. In 40% of women with HSK, the bifurcation of the vena cava was below the level of L5 in contrast to the 8% in the normal population (P < 0.001). In 60% of women with HSK, the inferior pole of the kidney was at or below L5. CONCLUSIONS: Ureteral, renal, and vascular anatomic alterations in women with HSK may make presacral surgical anatomy challenging by obscuring the anterior longitudinal ligament anchoring point. Preoperative imaging is warranted to determine the feasibility of female pelvic reconstructive surgery in HSK patients.


Assuntos
Aorta Abdominal/diagnóstico por imagem , Rim Fundido/diagnóstico por imagem , Rim , Pelve , Ureter/diagnóstico por imagem , Pontos de Referência Anatômicos , Variação Anatômica , Procedimentos Clínicos , Feminino , Humanos , Rim/anatomia & histologia , Rim/irrigação sanguínea , Rim/diagnóstico por imagem , Imagem por Ressonância Magnética/métodos , Pessoa de Meia-Idade , Pelve/diagnóstico por imagem , Pelve/cirurgia , Cuidados Pré-Operatórios/métodos , Procedimentos Cirúrgicos Reconstrutivos/métodos , Tomografia Computadorizada por Raios X/métodos
8.
Eur J Radiol ; 124: 108840, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31981879

RESUMO

PURPOSE: To establish an accurate and reliable equation for kidney depth estimation in adult patients from different Chinese geographical regions. METHOD: This multicenter study enrolled Eastern Asian Chinese patients with abdominal PET/CT scans at 26 imaging centers from six macro-regions across China in 3 years. Age, gender, height, weight, primary disease and its extent on PET scans of the participants were collected as potential predictive factors. Kidney depth on CT, defined as the average of the vertical distances from the posterior skin to the farthest anterior and closest posterior surfaces of each kidney, was measured as the standard reference. The new kidney depth model was constructed using a multiple regression model, and its performance was compared to those of three established models by computing the absolute value of estimation errors in comparison with CT-measured kidney depth. RESULTS: A total of 2502 patients were enrolled and classified into training (n=1653) and testing (n = 849) subsets. In the training subset, two kidney depth models were constructed: Left (cm): 0.013×age+0.117×gender-0.044×height+0.087×weight+7.951, Right (cm): 0.005×age+0.013×gender-0.035×height+0.082×weight+7.266 (weight: kg, height: cm, gender = 0 if female, 1 if male). In the testing subset, one-way analysis of variance showed that the estimation errors of the new models did not significantly differ among the 6 regions. Bland-Altman analysis determined that new equations had lower estimated biases (left: 0.039 cm, right: 0.018 cm) compared with other existing models. CONCLUSION: The new equations were highly accurate for kidney depth estimation in adults from all over China, with lower estimation errors compared to other established models.


Assuntos
Rim/anatomia & histologia , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons/métodos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Reprodutibilidade dos Testes , Tomografia Computadorizada por Raios X/métodos , Adulto Jovem
9.
Nephrol Dial Transplant ; 35(4): 640-647, 2020 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-30169833

RESUMO

BACKGROUND: The relationship of kidney size to ageing, kidney function and kidney disease risk factors is not fully understood. METHODS: Ultrasound length and parenchymal kidney volume were determined from a population-based sample of 3972 Sardinians (age range 18-100 years). We then identified the subset of 2256 'healthy' subjects to define age- and sex-specific reference ranges (2.5-97.5 percentile) of kidney volume. Logistic regression (accounting for family clustering) was used to identify the clinical characteristics associated with abnormally large kidneys or abnormally small kidneys. RESULTS: In the healthy subset, kidney volume and length increased up to the fourth to fifth decade of life followed by a progressive decrease in men, whereas there was a gradual kidney volume decrease throughout the lifespan of women. In the whole sample, independent predictors of lower kidney volume (<2.5 percentile for age and sex) were male sex, low body mass index, short height, low waist:hip ratio and high serum creatinine (SCr); the independent predictors of larger kidney volume (>97.5 percentile for age and sex) were younger age, female sex, diabetes, obesity, high height, high waist:hip ratio and lower SCr. Estimated heritability for kidney volume was 15%, and for length 27%; kidney volume correlated strongly with birthweight. CONCLUSIONS: Overall, in a general healthy population, kidney measures declined with age differently in men and women. The determinants of kidney parenchymal volume include genetic factors and modifiable clinical factors.


Assuntos
Envelhecimento , Peso ao Nascer , Índice de Massa Corporal , Diabetes Mellitus/fisiopatologia , Rim/anatomia & histologia , Obesidade/fisiopatologia , Insuficiência Renal Crônica/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Rim/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Insuficiência Renal Crônica/epidemiologia , Fatores de Risco , Fatores Sexuais , Ultrassonografia , Relação Cintura-Quadril , Adulto Jovem
10.
Folia Morphol (Warsz) ; 79(1): 93-97, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31106842

RESUMO

BACKGROUND: The purpose of this study was to assess the relationship between renal artery cross-section area and kidney volume with consideration of anatomical variants of renal arteries, sexual dimorphism and lateralisation. MATERIALS AND METHODS: Two hundred and two patients, 104 women and 98 men, aged 57.3 ± 16 years were examined using computed tomography angiography (CTA) of abdominal aorta for various reasons. The cross-section areas of renal arteries were measured automatically with a vessel tracking programme and summed up on each side in case of the presence of additional renal arteries. The kidneys were measured manually. RESULTS: Additional renal arteries (ARA) were found in 68 (33.7%) patients. Fifty-three (77.9%) of them had one, 11 (16.2%) two and 4 (5.9%) three ARAs. Bilateral ARAs occurred in 10 cases (14.7% patients with ARA). Proximal branching of renal artery occurred in 36 (8.4%) renal arteries. The cross-section area of the largest renal artery depended on the number of ipsilateral renal arteries. Mean cross-section area of the main left renal artery was larger than on the right side (28.52 mm2 vs. 25.36 mm2, p < 0.01) in the whole analysed group. Strong sexual dimorphism in renal artery cross-section area was observed (p < 0.01) in favour of men (31.3 mm2 in men and 22.9 mm2 in women). Mean total renal artery cross-section area has positively correlated with kidney volume (p < 10-13) in both sexes with Pearson correlation value of 0.5. CONCLUSIONS: The cross-section area of renal arteries correlated positively with kidney volume in both sexes. Presence of ARAs does not influence the sum of cross-section areas of renal arteries. In case of a difference between left and right renal artery cross-section area with symmetrical kidneys, it is necessary to look for ARA.


Assuntos
Rim/anatomia & histologia , Artéria Renal/anatomia & histologia , Adolescente , Adulto , Idoso de 80 Anos ou mais , Angiografia por Tomografia Computadorizada , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
11.
J Ultrasound Med ; 39(2): 223-230, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31418892

RESUMO

Ultrasound is commonly the first-line imaging modality for assessing the pediatric abdomen. An abnormal size of the liver, spleen, or kidneys may indicate disease, but the evaluation is challenging because the normal size changes with age. In addition, published normal value charts for children may vary by population and methods. In this systematic review, we summarized published data on the normal size of the pediatric liver, spleen, and kidneys as measured by ultrasound in which we found similar values across different populations, ages, and sexes.


Assuntos
Rim/anatomia & histologia , Rim/diagnóstico por imagem , Fígado/anatomia & histologia , Fígado/diagnóstico por imagem , Baço/anatomia & histologia , Baço/diagnóstico por imagem , Ultrassonografia/métodos , Criança , Humanos , Tamanho do Órgão , Valores de Referência
12.
Clin Nephrol ; 93(1): 31-35, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31448721

RESUMO

INTRODUCTION: Little information is available regarding the evaluation of renal volume in healthy Latin-American children of different ages. The objective of this work was to establish a predictive model of renal size (volume and length) and develop a web-based calculator. MATERIALS AND METHODS: A selective and representative sample was obtained randomly from the database of healthy children living in Resistencia city, Chaco, Argentina: a) the National Health Program for children under 6 years old; b) school children until 18 years old (primary and middle education). Renal dimensions were obtained by ultrasonography via a single experienced operator at the indicated site (schools or primary health care centers). Renal volume was calculated using Dinkel's formula. A multiple linear regression model was applied using potential predictors. The final model was implemented in a free web-based application. RESULTS: Random selection was made from the database to include 882 subjects with ages between 0.03 and 230.63 months. The data was divided into two sets (one for training and the other for model testing). The training set (423) included 212 (50%) females. Significant predictors included age, height, current weight and birth weight, and the interaction between age and present weight. Using the test dataset, both renal volume and length root mean square errors were 5.06 cm3 and 0.59 cm. CONCLUSION: The prediction model was accurate and allowed for the development a freely-available web app: Renal size prediction (https://porbm28.shinyapps.io/RenalVolume/). Once the models are validated by additional studies, the app could be a useful tool to predict renal volume and length in pediatric clinical practice.


Assuntos
Rim/anatomia & histologia , Adolescente , Criança , Pré-Escolar , Estudos Transversais , Feminino , Humanos , Lactente , Recém-Nascido , Rim/diagnóstico por imagem , Modelos Lineares , Masculino , Tamanho do Órgão , Ultrassonografia
13.
Vet Clin North Am Exot Anim Pract ; 23(1): 1-19, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31759441

RESUMO

The avian kidney contains both cortical or reptilian and medullary or mammalian nephrons. The kidney filters up to 11 times the total body water daily. Approximately 95% of this volume is reabsorbed by tubular reabsorption, which likely results from a change in the rate of filtration and/or the rate of reabsorption. These changes can result because of the antidiuretic hormone arginine vasotocin. The urinary concentrating ability generally varies inversely with body mass; however, birds can concentrate their urine, often at 2 to 3 times the osmolality of plasma. Further concentration of urine may occur by retroperistalsis.


Assuntos
Aves/fisiologia , Osmorregulação , Vasoconstritores/metabolismo , Vasotocina/metabolismo , Animais , Aves/anatomia & histologia , Rim/anatomia & histologia , Rim/fisiologia , Concentração Osmolar , Urina
14.
Vet Clin North Am Exot Anim Pract ; 23(1): 231-247, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31759450

RESUMO

The number of fish as pets far exceeds the populations of any other companion animal. As our knowledge of aquatic animal species and aquatic animal medicine continues to expand, veterinary expertise is becoming more critical to the client, researcher, fisheries biologist, aquarist, farmer, and fish hobbyist. Similar to other vertebrates, fish are susceptible to infectious and noninfectious renal disease. This article compares vertebrate renal anatomy and physiology and highlights some renal disease examples.


Assuntos
Doenças dos Peixes/diagnóstico , Nefropatias/veterinária , Animais , Doenças dos Peixes/fisiopatologia , Doenças dos Peixes/terapia , Peixes , Rim/anatomia & histologia , Rim/fisiopatologia , Nefropatias/diagnóstico , Nefropatias/fisiopatologia , Nefropatias/terapia , Vertebrados
15.
BMC Urol ; 19(1): 132, 2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31830961

RESUMO

BACKGROUND: To assess the impact of volumetry of perinephric fat (PNF) on the perioperative outcomes of robot-assisted partial nephrectomy (RAPN). METHODS: Between 2016 and 2019, a single surgeon performed RAPN for 128 patients with clinical T1a-b renal tumors at our institution, and the 70 most recent patients were included in this study to minimize the effects of surgical experience. PNF was defined as a fatty area around the kidney within the anatomical structures, including the lateroconal fascia, fusion fascia, psoas muscle, lumbar quadrate muscle and diaphragm, and its volume was calculated based on reconstructed three-dimensional computed tomography images using the SYNAPSE VINCENT system. RESULTS: In this series, the trifecta and MIC (margin, ischemia and complications) score system outcomes were achieved in 69 (98.6%) and 64 patients (91.4%), respectively. The median PNF volume in the 70 patients was 166.05 cm3, which was significantly correlated with both the body mass index (BMI) and Mayo adhesive probability (MAP) score (correlation coefficient = 0.68 and 0.74, respectively). There was no significant difference in the R.E.N.A.L. nephrometry score, PNF volume or console time during RAPN among 5 groups consisting of 14 consecutive patients. Of several factors examined, the console time was significantly affected by the sex, MAP score and PNF volume, and only the PNF volume was independently associated with the console time. CONCLUSION: Even if performed by an experienced robotic surgeon beyond the initial learning curve, the PNF volume may influence the console time during RAPN.


Assuntos
Tecido Adiposo/diagnóstico por imagem , Carcinoma de Células Renais/cirurgia , Neoplasias Renais/cirurgia , Rim/diagnóstico por imagem , Nefrectomia/métodos , Procedimentos Cirúrgicos Robóticos/métodos , Tecido Adiposo/anatomia & histologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Índice de Massa Corporal , Feminino , Humanos , Imageamento Tridimensional/métodos , Rim/anatomia & histologia , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada Multidetectores/métodos , Nefrectomia/estatística & dados numéricos , Duração da Cirurgia , Procedimentos Cirúrgicos Robóticos/estatística & dados numéricos , Aderências Teciduais
16.
PLoS One ; 14(11): e0225152, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31743376

RESUMO

BACKGROUND: Since the start of organ transplantation, hypothermia-forced hypometabolism has been the cornerstone in organ preservation. Cold preservation showed to protect against ischemia, although post-transplant injury still occurs and further improvement in preservation techniques is needed. We hypothesize that hydrogen sulphide can be used as such a new preservation method, by inducing a reversible hypometabolic state in human sized kidneys during normothermic machine perfusion. METHODS: Porcine kidneys were connected to an ex-vivo isolated, oxygen supplemented, normothermic blood perfusion set-up. Experimental kidneys (n = 5) received a 85mg NaHS infusion of 100 ppm and were compared to controls (n = 5). As a reflection of the cellular metabolism, oxygen consumption, mitochondrial activity and tissue ATP levels were measured. Kidney function was assessed by creatinine clearance and fractional excretion of sodium. To rule out potential structural and functional deterioration, kidneys were studied for biochemical markers and histology. RESULTS: Hydrogen sulphide strongly decreased oxygen consumption by 61%, which was associated with a marked decrease in mitochondrial activity/function, without directly affecting ATP levels. Renal biological markers, renal function and histology did not change after hydrogen sulphide treatment. CONCLUSION: In conclusion, we showed that hydrogen sulphide can induce a controllable hypometabolic state in a human sized organ, without damaging the organ itself and could thereby be a promising therapeutic alternative for cold preservation under normothermic conditions in renal transplantation.


Assuntos
Metabolismo Energético , Sulfeto de Hidrogênio/metabolismo , Rim/metabolismo , Animais , Biomarcadores , Metabolismo Energético/efeitos dos fármacos , Humanos , Sulfeto de Hidrogênio/farmacologia , Rim/anatomia & histologia , Rim/citologia , Testes de Função Renal , Mitocôndrias/metabolismo , Tamanho do Órgão , Consumo de Oxigênio , Suínos
17.
PLoS One ; 14(11): e0224364, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31682607

RESUMO

BACKGROUND: Kidney transplantation is the treatment of choice in patient with end stage chronic kidney disease, offering the best long term survival and greater Quality of Life in this group of patients. Graft volume was correlated with improved renal function in living donor transplantations. The primary aim of this study was to correlate renal volume adjusted to body surface area with renal function one year (estimated glomerular filtration rate; eGFR) after kidney transplantation. METHODS: This single-center, prospective cohort study included 256 patients who underwent kidney transplantation from January 2011 through December 2015 at Hospital das Clínicas de Botucatu-UNESP. We evaluated three kidney measurements during the bench surgery; the final graft volume was calculated using the ellipsoid formula and adjusted to body surface area. RESULTS: In the living donors there was positive correlation between adjusted graft volume and eGFR (r = 0.311, p = 0.008). Multivariate analysis revealed that low rejection rate and increased adjusted graft volume were independent factors correlated with eGFR. In deceased donors, there was no correlation between adjusted kidney volume and eGFR (r = 0.08, p = 0.279) in univariate analysis, but a multivariate analysis indicated that lower kidney donor profile index (KDPI), absence of rejection and high adjusted kidney volume were independent factors for better eGFR. CONCLUSION: Adjusted kidney volume was positively correlated with a satisfactory eGFR at one year after living donor and deceased donor transplantations.


Assuntos
Aloenxertos/anatomia & histologia , Taxa de Filtração Glomerular/fisiologia , Falência Renal Crônica/cirurgia , Transplante de Rim/métodos , Rim/anatomia & histologia , Adulto , Aloenxertos/fisiopatologia , Superfície Corporal , Feminino , Seguimentos , Rejeição de Enxerto/epidemiologia , Rejeição de Enxerto/etiologia , Rejeição de Enxerto/fisiopatologia , Sobrevivência de Enxerto , Humanos , Rim/fisiopatologia , Transplante de Rim/efeitos adversos , Doadores Vivos , Masculino , Pessoa de Meia-Idade , Tamanho do Órgão , Estudos Prospectivos , Qualidade de Vida , Resultado do Tratamento
18.
Dev Cell ; 51(3): 399-413.e7, 2019 11 04.
Artigo em Inglês | MEDLINE | ID: mdl-31689386

RESUMO

Chronic kidney disease affects 10% of the population with notable differences in ethnic and sex-related susceptibility to kidney injury and disease. Kidney dysfunction leads to significant morbidity and mortality and chronic disease in other organ systems. A mouse-organ-centered understanding underlies rapid progress in human disease modeling and cellular approaches to repair damaged systems. To enhance an understanding of the mammalian kidney, we combined anatomy-guided single-cell RNA sequencing of the adult male and female mouse kidney with in situ expression studies and cell lineage tracing. These studies reveal cell diversity and marked sex differences, distinct organization and cell composition of nephrons dependent on the time of nephron specification, and lineage convergence, in which contiguous functionally related cell types are specified from nephron and collecting system progenitor populations. A searchable database, Kidney Cell Explorer (https://cello.shinyapps.io/kidneycellexplorer/), enables gene-cell relationships to be viewed in the anatomical framework of the kidney.


Assuntos
Linhagem da Célula , Rim/citologia , Caracteres Sexuais , Análise de Célula Única , Animais , Células Epiteliais/citologia , Feminino , Rim/anatomia & histologia , Masculino , Camundongos , Néfrons/citologia , Fatores de Tempo
19.
Eur. j. anat ; 23(6): 459-463, nov. 2019. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-185089

RESUMO

Situs inversus viscerum (SIV) is a rare congenital anomaly, which is still an intriguing phenomenon to anatomists and physicians alike. A complete SIV is characterized by a left-right transposition and mirror image of all thoraco-abdominal organs and their vasculature. The present report is based on one case with complete SIV, which was observed during the routine educational dissections of cadavers in the authors' Anatomy Department. A transposition of all truncal organs and their vasculature, and several variations of arteries and veins were present. The right branch of the proper hepatic artery was replaced by an artery that emanated from the superior mesenteric artery. The latter also released the inferior mesenteric artery. Additionally, a left accessory renal artery ran anterior to the inferior caval vein and posterior to the ureter to enter the hilum of the left kidney. There was also a variation in the anterior-posterior arrangement of the hilar structures of the left kidney. Additionally, a globally enlarged heart with coronary artery by-passes, a replaced aortic valve and an aortic arch aneurysm was observed. This case report is unique, as it presents a previously unreported co-incidence of SIV and hepatic, intestinal and renal vascular anomalies. It is important for the surgeon to be aware of such variations while planning an abdominal surgery in patients with SIV


No disponible


Assuntos
Humanos , Masculino , Idoso , Situs Inversus , Ductos Biliares Intra-Hepáticos/anormalidades , Trato Gastrointestinal/anormalidades , Rim/anormalidades , Anomalias dos Vasos Coronários , Ductos Biliares Intra-Hepáticos/anatomia & histologia , Trato Gastrointestinal/anatomia & histologia , Rim/anatomia & histologia , Dissecação/métodos , Artérias Mesentéricas/anormalidades , Artérias Mesentéricas/anatomia & histologia , Cadáver , Vasos Coronários/anatomia & histologia
20.
Nutrients ; 11(10)2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31627407

RESUMO

Carp skin gelatine hydrolysate (CSGH) may be a possible bioactive peptide source, as promising antioxidant properties have been noted during in vivo testing. Hence, the present study focused on improving the bioavailability of the antioxidant peptides from CSGH and on the use of furcellaran (FUR), which can protect the biopeptides during digestion in the gastrointestinal tract. Therefore, in this study, microcapsules coated with furcellaran and containing CSGH cores were prepared. The structural properties of the sample were determined using FT-IR and SEM analysis. The antioxidant properties of hydrolysate, uncoated, and encapsulated samples were investigated. In vivo analysis included determination of its safety in an animal organism and evaluation of the lipid profile, antioxidant blood status, and mRNA expression of some genes involved in antioxidant status in Wistar rats. The results showed no adverse effects of microencapsulated protein hydrolysates in laboratory animals. Nonetheless, there was a statistically significant rise in the level of total antioxidant status blood serum among animals consuming CSGH and not inducing oxidative stress. This can be viewed as a promising indication of the positive effects of antioxidant properties tested in vivo. The process of CSGH microencapsulation in FUR cause a decrease in antioxidant hydrolysate activity, both in vitro, as well as in healthy Wistar rats. When considering the results of the presented diverse therapeutic potential, further research on CSGH being a potential bioactive peptide source used as a functional food or nutraceutical, but with a different microencapsulation coating, is encouraged.


Assuntos
Alginatos/química , Antioxidantes/química , Cápsulas/química , Carpas/metabolismo , Gomas Vegetais/química , Pele/química , Ração Animal , Animais , Antioxidantes/farmacocinética , Dieta/veterinária , Suplementos Nutricionais , Coração/anatomia & histologia , Coração/efeitos dos fármacos , Hidrólise , Rim/anatomia & histologia , Rim/efeitos dos fármacos , Metabolismo dos Lipídeos , Fígado/anatomia & histologia , Fígado/efeitos dos fármacos , Masculino , Tamanho do Órgão/efeitos dos fármacos , Ratos , Ratos Wistar , Pele/metabolismo , Ganho de Peso
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