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1.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(9): 990-995, 2019 Sep 28.
Artigo em Chinês | MEDLINE | ID: mdl-31645487

RESUMO

OBJECTIVE: To explore the renal metabolic markers relavant to the renal toxicity of diethylnitrosamine and the metabolic pathways involved in the renal metabolic markers.
 Methods: Nineteen Sprague Dawley rats were assigned into 2 groups: A normal control group (n=9) and a diethylnitrosamine (DEN) administration group (n=10). The rats in the normal control group were given sterilized water for free drinking. The rats in the DEN administration group were given 0.1 mg/mL DEN solution for free drinking. After 18 weeks, the kidney tissues were collected and tested for nuclear magnetic resonance detection and pathological examination.
 Results: The content of kidneys metabolites in the rats with the DEN administration was changed significantly. The levels of alanine, taurine, pyruvate, acetate, and choline were significantly reduced compared with rat in the normal control group, while the levels of creatine, glycine, TMAO, methionine, proline, lactate, valine, leucine and isoleucine were significantly increased.
 Conclusion: Metabolicomics studies have revealed significant differences in five metabolic pathways, including valine, leucine and isoleucine biosynthesis, glycine serine and threonine metabolism, pyruvate metabolism, glycolysis or gluconeogenesis, cysteine and methionine metabolism.


Assuntos
Alquilantes/toxicidade , Dietilnitrosamina/toxicidade , Rim/efeitos dos fármacos , Redes e Vias Metabólicas/efeitos dos fármacos , Animais , Glicina , Rim/fisiologia , Ratos , Ratos Sprague-Dawley
2.
Genes Dev ; 33(19-20): 1319-1345, 2019 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-31575677

RESUMO

There are now many reports of human kidney organoids generated via the directed differentiation of human pluripotent stem cells (PSCs) based on an existing understanding of mammalian kidney organogenesis. Such kidney organoids potentially represent tractable tools for the study of normal human development and disease with improvements in scale, structure, and functional maturation potentially providing future options for renal regeneration. The utility of such organotypic models, however, will ultimately be determined by their developmental accuracy. While initially inferred from mouse models, recent transcriptional analyses of human fetal kidney have provided greater insight into nephrogenesis. In this review, we discuss how well human kidney organoids model the human fetal kidney and how the remaining differences challenge their utility.


Assuntos
Rim/fisiologia , Modelos Biológicos , Organoides/fisiologia , Regulação da Expressão Gênica no Desenvolvimento , Humanos , Rim/citologia , Rim/embriologia , Rim/crescimento & desenvolvimento , Organoides/citologia
3.
Sheng Li Xue Bao ; 71(5): 799-805, 2019 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-31646334

RESUMO

Nephronectin (NPNT) is a novel extracellular matrix protein and a new ligand of integrin α8ß1. Recent studies showed that NPNT is highly expressed in kidney, lung, thyroid, etc, and it may play an important role in many pathological conditions. NPNT is involved in the process of kidney development and acute kidney injury, regulates proliferation and differentiation of osteoblast, and induces the vasculogenesis in vitro. NPNT may play a key role in pathological osteoporosis and therefore be a new therapeutic target of bone diseases. NPNT gene variants are not only associated with lung function, but also potentially implicated in chronic airway diseases development. Moreover, NPNT is also an important factor that mediates pathology of cardiac, epidermis, breast, liver and teeth diseases. In this paper, we reviewed some research progresses on the structure, distribution, physiological and pathophysiological functions of NPNT.


Assuntos
Proteínas da Matriz Extracelular/fisiologia , Rim/fisiologia , Osteoblastos/citologia , Diferenciação Celular , Proliferação de Células , Humanos , Osteoporose
4.
Br J Anaesth ; 123(5): 584-591, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31521337

RESUMO

BACKGROUND: The REnal Protection Against Ischaemia-Reperfusion in transplantation (REPAIR) RCT examined whether remote ischaemic preconditioning (RIPC) improved renal function after living-donor kidney transplantation. The primary endpoint, glomerular filtration rate (GFR), quantified by iohexol at 12 months, suggested that RIPC may confer longer-term benefit. Here, we present yearly follow-up data of estimated GFR for up to 5 yr after transplantation. METHODS: In this double-blind, factorial RCT, we enrolled 406 adult live donor kidney transplant donor-recipient pairs in 15 European transplant centres. RIPC was performed before induction of anaesthesia. RIPC consisted of four 5 min inflations of a BP cuff on the upper arm to 40 mm Hg above systolic BP separated by 5 min periods of cuff deflation. For sham RIPC, cuff inflation to 40 mm Hg was undertaken. Pairs were randomised to sham RIPC, early RIPC only (immediately pre-surgery), late RIPC only (24 h pre-surgery), or dual RIPC (early and late RIPC). The pre-specified secondary outcome of estimated GFR (eGFR) was calculated from serum creatinine measurements, using the Chronic Kidney Disease Epidemiology Collaboration equation. Predefined safety outcomes were mortality and graft loss. RESULTS: There was a sustained improvement in eGFR after early RIPC, compared with control from 3 months to 5 yr (adjusted mean difference: 4.71 ml min-1 (1.73 m)-2 [95% confidence interval, CI: 1.54-7.89]; P=0.004). Mortality and graft loss were similar between groups (RIPC: 20/205 [9.8%] vs control 24/201 [11.9%]; hazard ratio: 0.79 [95% CI: 0.43-1.43]). CONCLUSIONS: RIPC safely improves long-term kidney function after living-donor renal transplantation when administered before induction of anaesthesia. CLINICAL TRIAL REGISTRATION: ISRCTN30083294.


Assuntos
Precondicionamento Isquêmico/métodos , Transplante de Rim , Traumatismo por Reperfusão/prevenção & controle , Adolescente , Adulto , Idoso , Aloenxertos , Método Duplo-Cego , Europa (Continente) , Feminino , Seguimentos , Taxa de Filtração Glomerular/fisiologia , Humanos , Rim/fisiologia , Rim/cirurgia , Doadores Vivos , Masculino , Pessoa de Meia-Idade , Tempo , Resultado do Tratamento , Adulto Jovem
5.
Physiol Rev ; 99(3): 1575-1653, 2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-31215303

RESUMO

The identification of genes causing inherited kidney diseases yielded crucial insights in the molecular basis of disease and improved our understanding of physiological processes that operate in the kidney. Monogenic kidney disorders are caused by mutations in genes coding for a large variety of proteins including receptors, channels and transporters, enzymes, transcription factors, and structural components, operating in specialized cell types that perform highly regulated homeostatic functions. Common variants in some of these genes are also associated with complex traits, as evidenced by genome-wide association studies in the general population. In this review, we discuss how the molecular genetics of inherited disorders affecting different tubular segments of the nephron improved our understanding of various transport processes and of their involvement in homeostasis, while providing novel therapeutic targets. These include inherited disorders causing a dysfunction of the proximal tubule (renal Fanconi syndrome), with emphasis on epithelial differentiation and receptor-mediated endocytosis, or affecting the reabsorption of glucose, the handling of uric acid, and the reabsorption of sodium, calcium, and magnesium along the kidney tubule.


Assuntos
Nefropatias/genética , Nefropatias/fisiopatologia , Rim/fisiologia , Rim/fisiopatologia , Animais , Humanos , Doenças Raras
7.
Res Vet Sci ; 125: 162-169, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31233960

RESUMO

To date, 13 aquaporin isoforms (AQPs) have been discovered in mammals, of which as many as 9 are located in epithelial cells lining the individual sections of the nephron and collecting tubules. Detailed analysis of the location and expression of AQPs in the kidneys of laboratory animals and humans allowed to define the key role of these proteins in renal excretion of water and other small molecules. Unfortunately, despite the significant advances in knowledge in this area, still little is known about this subject in livestock, including cattle. Therefore, the aim of the study was to determine the expression and AQPs location in the nephron segment in the bovine kidney by immunohistochemistry and Western blot. The distribution of a total of 8 aquaporins was determined as a result of the conducted experiments. The results obtained in the present study clearly indicate that aquaporins in cattle are involved in the renal regulation of water excretion and maintenance of proper acid-base balance. Undoubtedly, changes in the distribution and expression of AQPs in bovine kidneys may be the cause of water balance disorders and disruption of the normal body fluid composition. Kidney diseases in cattle are poorly described in veterinary medicine. Knowledge of cellular location and expression of all AQPs in the bovine kidney under normal physiological condition allows a deeper understanding of the renal regulation of body homeostasis. It creates new perspective for diagnosis and pharmacotherapy in cattle in the future.


Assuntos
Aquaporinas/metabolismo , Bovinos/fisiologia , Rim/fisiologia , Animais , Western Blotting , Células Epiteliais/metabolismo , Regulação da Expressão Gênica/fisiologia , Homeostase , Humanos , Imuno-Histoquímica , Isoformas de Proteínas , Água/metabolismo
8.
Transplant Proc ; 51(5): 1314-1316, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31056244

RESUMO

INTRODUCTION: Renal scintigraphy is used to evaluate split renal function. A computed tomography (CT) examination is also carried out for donor safety and appropriate transplantation surgery, and the renal volume (CT volumetry) can be obtained at that time. In this study, we evaluated donor kidney function by inulin clearance (Cin) before and after donor nephrectomy in living donor renal transplantation, and the predictive role of CT volumetry was compared with diethylenetriamine pentaacetic acid (DTPA). METHOD: From November 2005 to April 2018, 34 cases of living donor transplantation conducted at Fukuoka University Hospital were retrospectively studied. The donated kidney weight was measured in 25 cases, and postoperative Cin was measured in 19 cases. RESULTS: The average donor age was 51.7 years old (from 35 to 71). Preoperative Cin and postoperative Cin of donors were 86.3 mL/min/1.73 m2 (from 59.5 to 138.3) and 52.3 (from 40.5 to 76.6), respectively. The average CT volumetry of donated kidneys was 153.9 mL (from 107.8 to 219.3). Correlations of weight and DTPA and CT volumetry of donated kidneys were r = 0.033 (P = .8770) and r = 0.763 (P < .0001), respectively. Correlations of glomerular filtration rate of DTPA and CT volumetry and Cin of postoperative donor residual kidneys were r = 0.66 (P = .002) and r = 0.555 (P = .014). CONCLUSION: There was a significant correlation between CT volumetry and the weight of the removed kidneys, and a correlation between Cin after donor nephrectomy and CT volumetry of the remaining kidneys, but it did not exceed the predictive role of DTPA. However, it was suggested that it is worthy to use as a preoperative examination for split renal function equivalent to DTPA.


Assuntos
Testes de Função Renal/métodos , Transplante de Rim/métodos , Rim/diagnóstico por imagem , Rim/fisiologia , Doadores Vivos , Adulto , Idoso , Tomografia Computadorizada de Feixe Cônico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nefrectomia/métodos , Cuidados Pré-Operatórios/métodos , Estudos Retrospectivos , Coleta de Tecidos e Órgãos/métodos
9.
Transplant Proc ; 51(5): 1306-1310, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31076152

RESUMO

BACKGROUND: Securing postdonation renal function in the lifetime of donors is a consequential subject for physicians, and precise prediction of postdonation renal function would be considerably beneficial when judging the feasibility of kidney donation. The aim of this study was to investigate the optimum model for predicting eGFR at 1 year after kidney donation. METHODS: We enrolled 101 living-related kidney donors for the development cohort and 44 for the external validation cohort. All patients in each cohort underwent thin-sliced (1 mm) enhanced computed tomography (CT) scans. We excluded individuals with diabetes, glucose intolerance, or albuminuria from this study. We evaluated preoperative factors including age, sex, hypertension, body mass index (BMI), serum uric acid, baseline eGFR, and body surface area (BSA)-adjusted preserved kidney volume (PKV) by using 3-dimensional reconstruction of thin-sliced enhanced CT images. To detect independent predictors, we performed multivariable regression analysis. RESULTS: The multivariable regression analysis revealed that age, BMI, predonation eGFR, and BSA-adjusted PKV were independent predictors of eGFR at 1 year after kidney donation (correlation coefficient: -0.15, -0.476, 0.521, 0.127, respectively). A strong correlation between predicted eGFR and observed eGFR was obtained in the development cohort (r = 0.839, P < .0001). The significance of this predictive model was also confirmed with the external validation cohort (r = 0.797, P < .0001). CONCLUSIONS: Age, BMI, predonation eGFR, and BSA-adjusted PKV may be useful for precisely predicting eGFR at 1 year after living kidney donation and be helpful to determine the feasibility of kidney donation from marginal donors.


Assuntos
Taxa de Filtração Glomerular , Transplante de Rim , Rim/fisiologia , Doadores Vivos , Adulto , Idoso , Índice de Massa Corporal , Estudos de Coortes , Feminino , Humanos , Transplante de Rim/métodos , Masculino , Pessoa de Meia-Idade , Nefrectomia/métodos , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodos
10.
Ter Arkh ; 91(1): 95-100, 2019 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-31090379

RESUMO

Generalized data on nephroprotective efficacy of pentoxifylline in chronic kidney disease (CKD) are presented. The potential of this drug in treating people suffering from CKD and cardiovascular diseases (CVD) with a high risk of developing the terminal stage of renal dysfunction is considered. Antiproteinuric, antifibrotic and anti-inflammatory effects of pentoxifylline significantly reduce the risk of progression of CKD and joining of CVD in the future. Efficacy in preventing the onset of the uremic stage of CKD, safety andapplicability at all stages of renal dysfunction development make pentoxifylline a very appealing drug not only for nephrologists but also for physicians. Keywords: chronic kidney disease, progression, pentoxifylline, nephroprotection, cardiovascular diseases.


Assuntos
Doenças Cardiovasculares/complicações , Rim/efeitos dos fármacos , Pentoxifilina/uso terapêutico , Insuficiência Renal Crônica/tratamento farmacológico , Progressão da Doença , Humanos , Rim/fisiologia , Insuficiência Renal Crônica/fisiopatologia , Fatores de Risco
11.
Adv Exp Med Biol ; 1132: 99-112, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31037629

RESUMO

Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function. Polycystic kidney disease (PKD), a genetic disorder, is characterized by the formation of numerous fluid-filled cysts in the kidneys. Periostin is secreted by the cyst epithelial cells and accumulates within the extracellular matrix adjacent to the cysts. In PKD mice, periostin overexpression accelerates cyst growth and contributes to structural changes in the kidneys, including interstitial fibrosis. Recent evidence suggests that periostin is a tissue repair molecule; however, its role in repair following acute kidney injury has not been investigated. It is thought that persistent expression of this protein in CKD contributes importantly to tubulointerstitial fibrosis and the progressive decline in renal function. Future studies to define the diverse actions of periostin during kidney injury may lead to effective therapies to slow PKD progression and possibly prevent the development of CKD. This chapter reviews the current literature on the expression of periostin in PKD and other forms of CKD, mechanisms for periostin stimulated cyst growth, its potential role in extracellular matrix production and renal fibrosis, and the evidence for periostin as a novel biomarker for kidney disease.


Assuntos
Moléculas de Adesão Celular/fisiologia , Rim/fisiologia , Insuficiência Renal Crônica/fisiopatologia , Animais , Matriz Extracelular , Fibrose , Humanos , Rim/patologia , Camundongos , Doenças Renais Policísticas/fisiopatologia
12.
J Trace Elem Med Biol ; 54: 36-43, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31109619

RESUMO

Data (N = 9882) from National Health and Nutrition Examination Survey for 2003-2014 for US adults aged ≥20 years were analyzed to evaluate the variabilities in concentrations of blood and urine lead across various stages of glomerular function. Those who had estimated glomerular filtration rate (eGFR) >90 mL/min/1.73 m2 were defined to be in glomerular function stage 1 (GF-1), those who had eGFR between 60 and 90 mL/min/1.73 m2 were defined to be in GF-2, those who had eGFR between 45 and 60 mL/min/1.73 m2 were defined to be in GF-3A, those who had eGFR between 15 and 45 mL/min/1.73 m2 were defined to be in GF-3B/4. There were consistent increases in adjusted geometric means (AGM) for both blood and urine lead from GF-1 to GF-3A even though increases were not uniform from one GF stage to another. For the total population, AGMs for blood lead were 1.23, 1.78, 2.25, and 2.25 µg/dL for GF-1, GF-2, GF-3A, and GF-3B/4 respectively. AGMs for urine lead were 0.49, 0.61, 0.68, and 0.47 µg/L for GF-1, GF-2, GF-3A, and GF-3B/4 respectively. Thus, from GF-3A to GF-3B/4, AGMs for both blood and urine lead decreased. However, percent increases from GF-1 to GF-3A for urine lead were smaller than for blood lead and percent decreases from GF-3A to GF-3B/4 for urine lead were larger than for blood lead. Females had lower AGMs than males for both blood and urine lead. Also, smoker-nonsmoker differences for blood lead narrowed as kidney function deteriorated but smoker-nonsmoker differences for urine cadmium lead as kidney function deteriorated. Smokers had sharper increases in AGMs for blood and urine lead than nonsmokers from GF-1 to GF-3A but at GF-3B/4, this difference was reduced to 0.17 µg/dL.


Assuntos
Taxa de Filtração Glomerular/fisiologia , Rim/metabolismo , Chumbo/sangue , Chumbo/urina , Adulto , Idoso , Cádmio/sangue , Cádmio/urina , Feminino , Humanos , Rim/fisiologia , Masculino , Pessoa de Meia-Idade , Fumar/efeitos adversos , Adulto Jovem
13.
J Sci Med Sport ; 22(9): 1004-1009, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31085130

RESUMO

OBJECTIVES: To investigate changes in renal status from exercise in the heat with acclimatisation and to evaluate surrogates markers of Acute Kidney Injury. DESIGN: Prospective observational cohort study. METHODS: 20 male volunteers performed 60 min standardised exercise in the heat, at baseline and on four subsequent occasions during a 23-day acclimatisation regimen. Blood was sampled before and after exercise for serum creatinine, copeptin, interleukin-6, normetanephrine and cortisol. Fractional excretion of sodium was calculated for corresponding urine samples. Ratings of Perceived Exertion were reported every 5 min during exercise. Acute Kidney Injury was defined as serum creatinine rise ≥26.5 µmol L-1 or fall in estimated glomerular filtration rate >25%. Predictive values of each candidate marker for developing Acute Kidney Injury were determined by ROC analysis. RESULTS: From baseline to Day 23, serum creatinine did not vary at rest, but showed a significant (P<0.05) reduction post-exercise (120 [102, 139] versus 102 [91, 112] µmol L-1). Acute Kidney Injury was common (26/100 exposures) and occurred most frequently in the unacclimatised state. Log-normalised fractional excretion of sodium showed a significant interaction (exercise by acclimatization day), with post-exercise values tending to rise with acclimatisation. Ratings of Perceived Exertion predicted AKI (AUC 0.76, 95% confidence interval 0.65-0.88), performing at least as well as biochemical markers. CONCLUSIONS: Heat acclimatization is associated with reduced markers of renal stress and AKI incidence, perhaps due to improved regional perfusion. Acclimatisation and monitoring Ratings of Perceived Exertion are practical, non-invasive measures that could help to reduce renal injury from exercise in the heat.


Assuntos
Aclimatação , Lesão Renal Aguda/diagnóstico , Exercício , Temperatura Alta , Rim/fisiologia , Lesão Renal Aguda/fisiopatologia , Adulto , Biomarcadores/sangue , Creatinina/sangue , Taxa de Filtração Glomerular , Glicopeptídeos/sangue , Humanos , Hidrocortisona/sangue , Interleucina-6/sangue , Rim/fisiopatologia , Masculino , Militares , Normetanefrina/sangue , Estudos Prospectivos , Estresse Fisiológico , Adulto Jovem
14.
Life Sci ; 227: 51-57, 2019 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-31004655

RESUMO

AIMS: Increasing evidence has shown the diagnostic value of miR-155 in organ transplantation. The dysregulation of miR-155 is reported to be associated with development of acute or chronic complications in solid organ transplant recipients. Here, we summarized related evidence to explore the correlation between the dysregulation of miR-155 and various allograft dysfunction in transplant recipients, and verified the dynamic change of miR-155 level in acute rejection (AR) using a rat renal transplantation model. MAIN METHODS: Eligible studies were retrieved from PubMed, Embase, and Cochrane Library databases. A meta-analysis method was performed to evaluate the diagnostic value of miR-155 in transplant recipients. Furthermore, the F344-Lewis rat renal transplantation model was established to validate the dynamic change of miR-155 expression during AR. KEY FINDINGS: A total of 275 transplant patients, including renal, heart, and lung transplantation from 6 studies were analysed. The pooled SEN of miR-155 was 0.87 (95% CI, 0.78-0.93), the pooled SPE was 0.76 (95% CI, 0.63-0.85), the pooled PLR was 3.6 (95% CI, 2.2-5.8), the pooled NLR was 0.17 (95% CI, 0.09-0.31), the pooled DOR was 17.31 (95% CI, 7.20-41.65) and pooled AUC was 0.89 (95% CI, 0.86-0.92). The rat renal transplantation model (n = 24) and control model (n = 15) were successfully established. Expression of miR-155 in plasma was significantly increased in 7 d and 9 d post-transplantation compared to the control group (P < 0.05), and was consistent with the dynamic change of AR degree. SIGNIFICANCE: miR-155 is a potential biomarker for monitoring the abnormal allograft status in solid organ transplantation.


Assuntos
Rejeição de Enxerto/genética , MicroRNAs/genética , Aloenxertos/fisiologia , Animais , Biomarcadores/sangue , Humanos , Rim/fisiologia , Transplante de Rim/métodos , Masculino , MicroRNAs/sangue , MicroRNAs/fisiologia , Modelos Animais , Curva ROC , Ratos , Ratos Endogâmicos F344 , Ratos Endogâmicos Lew , Transplante Homólogo/métodos
15.
Zhonghua Liu Xing Bing Xue Za Zhi ; 40(4): 427-432, 2019 Apr 10.
Artigo em Chinês | MEDLINE | ID: mdl-31006203

RESUMO

Objective: To explore the association between dietary pattern which benefit for normal kidney function and the risk of cognitive decline or impairment in the elderly. Methods: In 2015, subjects aged 60 and over from four counties in the Nutrition and Chronic Disease Family Cohort project, were followed up in 2017. Cognitive function was repeatedly assessed, using the Mini Mental State Evaluation (MMSE) scale. Dietary pattern that benefit for normal kidney function was extracted, using the reduced rank regression method and followed by logistic regression models to explore the associations between scores that showing the kidney function on dietary patterns and the risk of cognitive deterioration and impairment in two years among those who were with normal cognition in 2015. Results: Dietary pattern that benefit for normal kidney function, was characterized by high consumption of cereal, vegetables, legume and fruits but with less meat and soy products. Comparing with the group with lowest score quartile on this dietary pattern, the risk of cognitive deterioration in the highest quartile group was significantly low (P<0.01) in two years, with an odds ratio as 0.57 (95%CI: 0.37-0.85). Linear trend was also obviously visible (P=0.007) in this group. The ones at the highest quartile group among the normal cognition ones in 2015, the risk of cognitive impairment also significantly reduced (P<0.05) in two years time, with an odds ratio as 0.52 (95%CI: 0.29-0.93). Also, linear trend could obviously be seen (P=0.01). Conclusion: Dietary pattern that benefit for normal kidney function was both inversely associated with cognitive deterioration and impairment, in two years.


Assuntos
Cognição/fisiologia , Comportamento Alimentar , Rim/fisiologia , Idoso , Estudos de Coortes , Humanos , Pessoa de Meia-Idade , Fatores de Risco , Verduras
17.
Lipids Health Dis ; 18(1): 102, 2019 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-31010430

RESUMO

BACKGROUND: We previously reported that the consumption of 8 g of protein per day in the form of lactic-fermented egg white (LAFEW) improves visceral fat obesity. In this study, we investigated the minimum effective intake of LAFEW for visceral fat reduction in the Japanese males with mild obesity. METHODS: Twenty-two Japanese adult males with a Body mass index (BMI) ≥24 and a waist circumference ≥ 85 cm were included in this study. The subjects were divided into three groups, that is, control group, LAFEW 6 g group, and LAFEW 8 g group. The LAFEW 6 and 8 g groups consumed 6 and 8 g, respectively, of egg white protein (EWP) in a drink at breakfast for 8 weeks, whereas the control group consumed a drink containing 8 g of milk whey protein. Body weight, body fat percentage, abdominal circumference, and visceral fat (VF) area around the navel were measured at 0 and 8 weeks after initiating the consumption. RESULTS: No changes in body weight or body fat percentage were observed in any of the groups. No significant differences between the pre- and posttreatment measurements were found in the VF area around the navel in the control group and the LAFEW 6 g group. In the LAFEW 8 g group, the VF area had decreased significantly after 8 weeks of consumption, when compared to that before consumption, and the average observed decrease (Δcm2) was 13.2 ± 4.7 cm2. Among the subjects with an initial BMI > 25, the VF area was significantly smaller in the LAFEW 8 group, when compared to the week 0 values and those in the control group. Visceral fat/subcutaneous fat values in the LAFEW 8 group were also significantly smaller than those in the control group or at week 0. CONCLUSION: The results suggested that the minimum effective intake of EWP in the LAFEW to reduce the VF area in the Japanese men is 8 g. TRIAL REGISTRATION: This clinical trial was retrospectively registered with the University hospital Medical Information Network (UMIN) Center, ( UMIN000031681 ; registered on 12/03/2018).


Assuntos
Clara de Ovo/química , Fermentação , Gordura Intra-Abdominal/efeitos dos fármacos , Ácido Láctico/farmacologia , Adulto , Animais , Galinhas , Dieta , Método Duplo-Cego , Humanos , Gordura Intra-Abdominal/diagnóstico por imagem , Rim/efeitos dos fármacos , Rim/fisiologia , Lipídeos/sangue , Fígado/efeitos dos fármacos , Fígado/fisiologia , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Tomografia Computadorizada por Raios X
18.
Phytother Res ; 33(6): 1648-1657, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30942510

RESUMO

Diabetes mellitus is a metabolic disease that manifested as hyperglycemia due to the defect in secretion or function of insulin. Studies have shown that saffron and its derivatives cause a significant reduction in plasma glucose levels in experimental models. The purpose of this study was to investigate the effect of the saffron extract on fasting plasma glucose (FPG), glycated hemoglobin level (HbA1c), lipid profile, liver enzymes, and renal function tests in type 2 diabetic patients. In this double-blind randomized clinical trial, 64 type 2 diabetic patients who were on oral anti-diabetic drugs were examined. Participants received either 15 mg of saffron or placebo capsules (two pills per day) for 3 months. Anthropometric indices, dietary intake, FPG, HbA1c, lipid profiles, liver enzymes (ALT, AST, ALP), and renal function (BUN, Cr.) tests were measured pre and post intervention after 3 months. Independent t test and paired t test were used for data analysis. After 3-months intervention, mean difference of FPG, Cholesterol, LDL-c, and LDL/HDL ratio between two groups showed significant reduction(p < 0.0001), but HbA1c, HDL-C, API, TG showed no significant differences (p > 0.05). In saffron group, FPG, HbA1c, cholesterol, LDL-c, and LDL/HDL ratio decreased significantly after 3-months intervention compare with baseline (p < 0.0001).


Assuntos
Crocus/química , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/metabolismo , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Adulto , Idoso , Glicemia/análise , Glicemia/efeitos dos fármacos , Glicemia/metabolismo , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/fisiopatologia , Método Duplo-Cego , Etanol/química , Jejum/sangue , Feminino , Hemoglobina A Glicada/efeitos dos fármacos , Hemoglobina A Glicada/metabolismo , Humanos , Hipoglicemiantes/uso terapêutico , Insulina/sangue , Rim/fisiologia , Testes de Função Renal , Metabolismo dos Lipídeos/efeitos dos fármacos , Lipídeos/sangue , Fígado/metabolismo , Fígado/fisiologia , Testes de Função Hepática , Masculino , Pessoa de Meia-Idade , Extratos Vegetais/química , Água/química
19.
Biomed Res Int ; 2019: 1321287, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31016183

RESUMO

SERPINA1 is a member of serine protease inhibitors and is increasingly considered to be a regulator of innate immunity in human and animals. However, the expression and function of SERPINA1 gene in immune defense against viral infection remain unknown in ducks. The full-length du SERPINA1 cDNA sequence was obtained using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). It contained 1457 nucleotide, including 47-bp 5' UTR, 135-bp 3' UTR, and 1275-bp open reading frame (ORF), and encodes a 424-amino acid protein. Then, the tissue expression profile of du SERPINA1 gene was determined. Real-time quantitative polymerase chain reaction (real-time qPCR) analysis revealed that du SERPINA1 mRNA is ubiquitous in various tissues, but higher expression levels were observed in lung and liver tissues. In addition, the expression pattern was investigated when the ducklings were challenged with duck hepatitis virus 1(DHV-1) and polyriboinosinic polyribocytidylic acid (poly I:C). After DHV-1 injection or poly I:C treatment, du SERPINA1 mRNA was up-regulated in the liver and kidney tissues. However, the peak time in two tissues was not consistent. In kidney, the expression lever of SERPINA1 increased immediately after the treatment while in liver tissue it kept steady until 12 h post-infection. Our results indicate that SERPINA1 has an active role in the antiviral response, and thus improve our understanding of the role of this protein.


Assuntos
Patos/genética , Expressão Gênica/genética , alfa 1-Antitripsina/genética , Regiões 3' não Traduzidas/genética , Regiões 5' não Traduzidas/genética , Sequência de Aminoácidos , Animais , Clonagem Molecular/métodos , DNA Complementar/genética , Vírus da Hepatite do Pato/genética , Rim/fisiologia , Fígado/fisiologia , Fases de Leitura Aberta/genética , Filogenia , RNA Mensageiro/genética , Alinhamento de Sequência , Análise de Sequência de DNA/métodos , Regulação para Cima/genética
20.
Biomed Res Int ; 2019: 5280514, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31032350

RESUMO

The study aims to investigate the effects of the alcohol extract of Coreopsis tinctoria Nutt (AC) on diabetic nephropathy (DN) mice. A total of 30 db/db (DN) mice were divided into 3 groups, which were treated with AC (300 mg/kg/day), metformin (180 mg/kg/day), or saline by gavage for 10 weeks. Ten db/m mice treated with saline were used as normal control (NC group). Body weight (BW) and fasting blood glucose (FBG), HbA1c, 24 h urinary albumin excretion (UAE), and renal pathological fibrosis were analyzed. Expression of miR-192, miR-200b, and proteins in the PTEN/PI3K/AKT pathway was analyzed by qPCR or western blot. The DN mice had significantly higher BW, FBG, and 24 h UAE, as well as more severe pathological fibrosis when compared with NC. Treatment of AC could decrease BW, FBG, and 24 h UAE and alleviated kidney damage. Compared with the NC group, expressions of miR-192 and miR-200b were increased, whereas their target proteins (ZEB2 and PTEN) were reduced in the kidneys of DN mice, which further modulated the expression of their downstream proteins PI3K p85α, P-AKT, P-smad3, and COL4 α1; these proteins were increased in the kidneys of DN mice. In contrast, AC treatment reversed the expression changes of these proteins. These findings demonstrate that AC may protect the kidneys of DN mice by decreasing miR-192 and miR-200b, which could further regulate their target gene expression and modulate the activity of the PTEN/PI3K/AKT pathway to reduce the degree of renal fibrosis.


Assuntos
Coreopsis/química , Diabetes Mellitus/tratamento farmacológico , Nefropatias Diabéticas/tratamento farmacológico , MicroRNAs/genética , Albuminúria/urina , Álcoois/química , Animais , Glicemia/isolamento & purificação , Diabetes Mellitus/genética , Diabetes Mellitus/patologia , Diabetes Mellitus/urina , Nefropatias Diabéticas/genética , Nefropatias Diabéticas/patologia , Nefropatias Diabéticas/urina , Modelos Animais de Doenças , Regulação da Expressão Gênica/efeitos dos fármacos , Hemoglobina A Glicada/isolamento & purificação , Humanos , Rim/efeitos dos fármacos , Rim/fisiologia , Camundongos , Camundongos Endogâmicos NOD , PTEN Fosfo-Hidrolase/genética , Extratos Vegetais/administração & dosagem , Extratos Vegetais/química , Proteínas Proto-Oncogênicas c-akt/genética , Transdução de Sinais/efeitos dos fármacos , Homeobox 2 de Ligação a E-box com Dedos de Zinco/genética
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