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1.
Arch Virol ; 165(6): 1333-1342, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32266552

RESUMO

Equine infectious anemia (EIA), a disease caused by equine infectious anemia virus (EIAV), is considered an obstacle to the development of the horse industry. There is no treatment or vaccine available for EIA, and its pathogenesis, as well as the immune response against the virus, is not fully understood. Therefore, an immunohistochemistry assay was developed for the detection of viral antigens in tissues of equids naturally infected with EIAV. Sections of organs of six equids from Apodi-RN, Brazil, that tested positive for EIA by serological tests (ELISA and AGID) were fixed in 10% formalin solution and embedded in paraffin. Immunohistochemistry was performed using a polyclonal anti-EIAV antibody. EIAV antigens were observed in red spleen pulp cells and hepatic sinusoids, as well as bronchiolar and alveolar epithelial cells of the lungs and proximal and distal tubules of the kidneys. The presence of EIAV in the spleen and liver was expected due to viral tropism by macrophages, which are abundantly present in these organs. However, EIAV was also found in lung and kidney epithelial cells, indicating that the virus infects cell types other than macrophages. In conclusion, the immunohistochemical assay standardized in this study was able to detect EIAV antigens in spleen, liver, kidney and lung cells from naturally infected EIAV equids. Immunostaining observed in the spleen confirms viral tropism by mononuclear phagocytes; however, the presence of EIAV in lung and kidney epithelial cells indicates that virus may be eliminated in urine and/or oronasal secretions, suggesting new routes for viral excretion.


Assuntos
Anemia Infecciosa Equina/virologia , Vírus da Anemia Infecciosa Equina/isolamento & purificação , Animais , Antígenos Virais/análise , Brasil , DNA Viral/genética , Células Epiteliais/patologia , Células Epiteliais/virologia , Anemia Infecciosa Equina/imunologia , Anemia Infecciosa Equina/patologia , Cavalos/virologia , Vírus da Anemia Infecciosa Equina/classificação , Rim/patologia , Rim/virologia , Leucócitos Mononucleares/virologia , Fígado/patologia , Fígado/virologia , Pulmão/patologia , Pulmão/virologia , Reação em Cadeia da Polimerase , Testes Sorológicos , Baço/patologia , Baço/virologia
2.
Cell ; 181(4): 905-913.e7, 2020 05 14.
Artigo em Inglês | MEDLINE | ID: mdl-32333836

RESUMO

We have previously provided the first genetic evidence that angiotensin converting enzyme 2 (ACE2) is the critical receptor for severe acute respiratory syndrome coronavirus (SARS-CoV), and ACE2 protects the lung from injury, providing a molecular explanation for the severe lung failure and death due to SARS-CoV infections. ACE2 has now also been identified as a key receptor for SARS-CoV-2 infections, and it has been proposed that inhibiting this interaction might be used in treating patients with COVID-19. However, it is not known whether human recombinant soluble ACE2 (hrsACE2) blocks growth of SARS-CoV-2. Here, we show that clinical grade hrsACE2 reduced SARS-CoV-2 recovery from Vero cells by a factor of 1,000-5,000. An equivalent mouse rsACE2 had no effect. We also show that SARS-CoV-2 can directly infect engineered human blood vessel organoids and human kidney organoids, which can be inhibited by hrsACE2. These data demonstrate that hrsACE2 can significantly block early stages of SARS-CoV-2 infections.


Assuntos
Betacoronavirus/efeitos dos fármacos , Infecções por Coronavirus/tratamento farmacológico , Peptidil Dipeptidase A/farmacologia , Pneumonia Viral/tratamento farmacológico , Proteínas Recombinantes/farmacologia , Animais , Betacoronavirus/genética , Betacoronavirus/isolamento & purificação , Betacoronavirus/ultraestrutura , Vasos Sanguíneos/virologia , Chlorocebus aethiops , Humanos , Rim/citologia , Rim/virologia , Camundongos , Organoides/virologia , Pandemias , Peptidil Dipeptidase A/genética , Peptidil Dipeptidase A/metabolismo , Receptores Virais/metabolismo , Glicoproteína da Espícula de Coronavírus/metabolismo , Células Vero
3.
Rev Med Suisse ; 16(N° 691-2): 842-844, 2020 Apr 29.
Artigo em Francês | MEDLINE | ID: mdl-32348049

RESUMO

During the actual pandemic of COVID-19, it has become clear that the virus causing this devastating disease, SARS-CoV2, targets not only the lungs but also other organs. In this article, we discuss the known or suspected interactions between the virus and the kidneys, as well as their clinical presentations. We also discuss how the pandemic has altered the activities of nephrologists and the logistics of a Swiss dialysis center.


Assuntos
Betacoronavirus , Infecções por Coronavirus/complicações , Nefropatias , Rim/virologia , Pandemias , Pneumonia Viral/complicações , Infecções por Coronavirus/diagnóstico , Humanos , Nefropatias/virologia , Nefrologistas , Pneumonia Viral/diagnóstico
4.
Nephron ; 144(5): 213-221, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32203970

RESUMO

Here, we review the most recent findings on the effects of SARS-CoV-2 infection on kidney diseases, including acute kidney injury, and examine the potential effects of ARBs on the outcomes of patients with COVID-19. Lastly, we discuss the clinical management of COVID-19 patients with existing chronic renal disorders, particularly those in dialysis and with kidney transplants.


Assuntos
Angiotensinas/antagonistas & inibidores , Infecções por Coronavirus/complicações , Nefropatias/complicações , Nefropatias/tratamento farmacológico , Pneumonia Viral/complicações , Antagonistas de Receptores de Angiotensina , Inibidores da Enzima Conversora de Angiotensina , Betacoronavirus/fisiologia , Humanos , Rim/virologia , Transplante de Rim , Nefrologistas , Pandemias , Peptidil Dipeptidase A , Diálise Renal , Replicação Viral
5.
PLoS Pathog ; 16(1): e1008262, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31971979

RESUMO

Mouse kidney parvovirus (MKPV) is a member of the provisional genus Chapparvovirus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four major MKPV transcripts, created by alternative splicing, to a common initiator region, and use mass spectrometry to identify "p10" and "p15" as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and the splicing-dependent putative accessory protein NS2 are conserved in all near-complete amniote chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropic and that the bat chapparvovirus DrPV-1 and a non-human primate chapparvovirus, CKPV, are also found in the kidneys of their hosts. We propose, therefore, that many mammal chapparvoviruses are likely to be nephrotropic.


Assuntos
Rim/virologia , Infecções por Parvoviridae/veterinária , Infecções por Parvoviridae/virologia , Parvovirinae/fisiologia , Doenças dos Roedores/virologia , Proteínas Virais/metabolismo , Tropismo Viral , Animais , Humanos , Camundongos , Parvovirinae/genética , Proteínas Virais/genética
6.
Arch Virol ; 165(1): 43-51, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31676996

RESUMO

Inclusion body hepatitis (IBH), hydropericardium syndrome (HS), and gizzard erosion (GE) are all economically important diseases in the poultry industry worldwide and are all caused by fowl aviadenovirus (FAdV). It is important to identify the serotype of the virus to differentiate these diseases. In the present study, a total of six recent FAdV serotypes were isolated and identified in broiler and broiler-breeder flocks in Izmir, Manisa, and Aydin provinces of the Aegean region of Turkey between January and March 2019. The viruses were isolated from livers and pooled organs of chickens using primary chicken embryo kidney cell cultures (CEKC). Virus isolates were identified by PCR amplification of the loop 1 (L1) variable region of the hexon gene followed by Sanger sequencing. Sequence analysis revealed the presence of both FAdV-D (serotype 11) and FAdV-E (serotype 8b). The viruses that were isolated were associated with IBH, which is typically characterized by gross lesions such as enlarged and pale yellow liver with multiple petechial hemorrhages. Histopathological examination also showed necrotizing hepatitis with intranuclear inclusion bodies in hepatocytes. This study is the first report of the isolation and identification of FAdV serotypes associated with IBH in commercial broilers and broiler-breeder flocks in Turkey. The results of sequence analysis showed that FAdV-8b and FAdV-11 were the circulating serotypes that caused recent field outbreaks of IBH in the Aegean region between January and March, 2019.


Assuntos
Infecções por Adenoviridae/virologia , Aviadenovirus/classificação , Doenças das Aves Domésticas/virologia , Análise de Sequência de DNA/métodos , Animais , Aviadenovirus/genética , Aviadenovirus/isolamento & purificação , Células Cultivadas , Galinhas , Corpos de Inclusão/virologia , Rim/citologia , Rim/virologia , Fígado/virologia , Filogenia , Sorotipagem , Turquia
7.
Ren Fail ; 41(1): 855-861, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31535918

RESUMO

Objectives: To investigate the immunohistochemical features of different stages of BK virus allograft nephropathy (BKVN) and further elucidate the underlying immunological mechanism involved in the evolution of BKVN. Methods: Fifty-two renal transplant recipients with biopsy proven BKVN were retrospectively selected. According to the third edition of the American Society of Transplantation Infection guidelines, 10 patients were categorized as having mild BKVN (stage A), 25 were moderate (stage B) and 17 were severe (stage C). The differential infiltrations of CD3+ (T lymphocytes), CD4+ (helper T lymphocytes), CD8+ (cytotoxic T lymphocytes), CD20+ (B lymphocytes), CD68+ (macrophages) and CD138+ (plasma cells) cells and the expression of interleukin-2 receptor (IL-2R) and human leukocyte antigen DR (HLA-DR) were compared among the three groups. Results: CD3+, CD4+, CD8+, CD20+, CD138+ and CD68+ cells infiltrations, IL-2R and HLA-DR expression were positive in the BKVN patients. Moreover, with increasing stages of BKVN, the numbers of positively stained inflammatory cells and the expression of IL-2R were significantly increased in the severe group compared to the mild group, whereas no statistically significant differences were observed with regard to HLA-DR expression. Eosinophil and neutrophil infiltration could also be observed in moderate to advanced BKVN. Conclusion: Renal allograft damage caused by BKVN involved T lymphocyte-, B lymphocyte- and mononuclear macrophage-mediated immune responses. Inflammatory cell infiltrations in the renal allograft were probably the driving force for BKVN progression. Additionally, eosinophils and neutrophils may be involved in the pathophysiological mechanism of BKVN.


Assuntos
Vírus BK/imunologia , Rejeição de Enxerto/imunologia , Transplante de Rim/efeitos adversos , Infecções por Polyomavirus/imunologia , Infecções Tumorais por Vírus/imunologia , Adulto , Aloenxertos/imunologia , Aloenxertos/patologia , Aloenxertos/virologia , Vírus BK/isolamento & purificação , Biópsia , Feminino , Rejeição de Enxerto/diagnóstico , Rejeição de Enxerto/virologia , Humanos , Imunidade Celular , Imunofenotipagem , Rim/imunologia , Rim/patologia , Rim/virologia , Masculino , Pessoa de Meia-Idade , Infecções por Polyomavirus/diagnóstico , Infecções por Polyomavirus/virologia , Estudos Retrospectivos , Índice de Gravidade de Doença , Transplante Homólogo , Infecções Tumorais por Vírus/diagnóstico , Infecções Tumorais por Vírus/virologia
8.
J Zhejiang Univ Sci B ; 20(9): 740-752, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31379144

RESUMO

Fowl adenovirus serotype 4 (FAdV-4) strain SD1511 was isolated from chickens with severe inclusion body hepatitis and hydropericardium syndrome in Shandong Province, China. The isolate was cultured in primary chicken embryo kidney cells. A study of pathogenicity indicated that SD1511 readily infected 7-35-d-old chickens by intramuscular injection and intranasal and oral routes, causing 50%-100% mortality. The 35-d-old chickens suffered more severe infection than 7- and 21-d-old chickens with mortality highest in the intramuscular injection group. The serum from surviving chickens showed potent viral neutralizing capability. The complete genome of SD1511 was sequenced and analyzed. The strain was found to belong to the FAdV-4 cluster with more than 99% identity with the virulent FAdV-4 strains isolated in China in recent years except for some distinct variations, including deletions of open reading frame 27 (ORF27), ORF48, and part of ORF19. Our findings suggest that SD1511 might be used as a prototype strain for the study of pathogenesis and vaccine development.


Assuntos
Aviadenovirus/genética , Aviadenovirus/patogenicidade , Rim/virologia , Fígado/virologia , Doenças das Aves Domésticas/virologia , Viroses/veterinária , Animais , Anticorpos Neutralizantes , Linhagem Celular , Embrião de Galinha/virologia , Galinhas/virologia , China , Deleção de Genes , Variação Genética , Genoma , Genoma Viral , Genômica , Rim/embriologia , Fases de Leitura Aberta , Sorogrupo , Carga Viral , Virulência , Viroses/virologia
9.
Transplant Proc ; 51(6): 1801-1809, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31399166

RESUMO

BK viremia (BKV) is a recognized and potentially serious problem in renal transplantation. The risk factors and the impact of BKV on renal allograft and patient survival are controversial. This study reports an 8-year, single-center experience on the prevalence, risk factors, and outcomes of BKV in kidney transplant recipients. This is a retrospective analysis of all patients who received a kidney transplant at the University of Kentucky and had BK viral titers available from 2009 to 2017. BKV was defined by a polymerase chain reaction viral load of ≥ 10,000 copies per mL. Demographic, clinical, and laboratory data generated during routine outpatient follow up and inpatients records were collected. Independent risk factors for BKV were determined using uni- and multivariate analysis. Graft and patient survival was compared using Kaplan-Meier analysis, and the severity of polyomavirus nephropathy on biopsy was scored using the Banff 2017 classification. We identified 122 BK positive (19%) and 527 BK negative (81%) patients. BKV developed after a median of 115 days (range, 80-249 days) following kidney transplantation. The 1-, 5-, and 10-year graft survival was 97%, 75%, and 33% in the BKV group and 96%, 85%, and 71% in the BK negative group, respectively. Likewise, the 1-, 5-, and 10-year patient survival was 98%, 84%, and 52% in the BKV group and 98%, 92%, and 84% in the BK negative group. Male sex, age at transplantation, maintenance steroids, and alemtuzumab induction were associated with developing BKV in the multivariate analysis. We concluded that BKV is not uncommon after renal transplantation. The determinants for BKV are male sex, older transplant recipients, and maintenance steroids. BKV adversely affected graft and patient survival. A unified approach for BKV and polyomavirus nephropathy treatment is needed.


Assuntos
Vírus BK , Nefropatias/virologia , Transplante de Rim/efeitos adversos , Infecções por Polyomavirus/virologia , Complicações Pós-Operatórias/virologia , Infecções Tumorais por Vírus/virologia , Viremia/virologia , Adulto , Biópsia , Feminino , Sobrevivência de Enxerto , Humanos , Estimativa de Kaplan-Meier , Rim/virologia , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Prevalência , Estudos Retrospectivos , Fatores de Risco , Transplante Homólogo , Carga Viral
10.
BMC Res Notes ; 12(1): 466, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31366401

RESUMO

OBJECTIVE: HIV positive individuals infected with viral hepatitis B (HBV) or C (HCV) are at an increased risk of progression to kidney and liver failures. Therefore, prior to initiation of antiretroviral therapy, early diagnosis and initiation of appropriate treatment protocols are imperative for co-infected individuals. This study evaluated the prevalence of HBV and HCV, and extent of liver and renal dysfunction among 90 newly diagnosed HIV patients attending the Cape Coast Teaching Hospital HIV clinic. RESULTS: Levels of alanine aminotransferase, aspartate-platelet ratio index and estimated glomerular filtration rate were used respectively to diagnose hepatotoxicity, liver fibrosis and chronic kidney disease (CKD). Association analyses were evaluated by Pearson's Chi-square test or Fisher's exact test and considered significant at p < 0.05. Using rapid diagnostic tests, 75.6% (n = 68) had HIV1 mono-infection, 24.4% (n = 22) had HIV1/HBV co-infection while 0.0% (n = 0) had HIV1/HCV co-infection. The prevalence of hepatotoxicity, liver fibrosis, and CKD were 7.8% (n = 7), 2.2% (n = 2), and 15.5% (n = 14) respectively. Similar proportions of HIV1/HBV and HIV1 were diagnosed with liver fibrosis (p = 0.431). In relation to hepatotoxicity Grade, a high proportion of HIV1/HBV were diagnosed with Grade 2 (p = 0.042). Also, severely reduced kidney function (CKD stage 4) was observed in only HIV1/HBV (n = 2, 9.1%, p = 0.053).


Assuntos
Infecções por HIV/fisiopatologia , Hepatite B/fisiopatologia , Hepatite C/fisiopatologia , Rim/fisiopatologia , Cirrose Hepática/fisiopatologia , Fígado/fisiopatologia , Insuficiência Renal Crônica/fisiopatologia , Adulto , Alanina Transaminase/sangue , Ácido Aspártico/sangue , Plaquetas/patologia , Coinfecção , Estudos Transversais , Feminino , Gana/epidemiologia , Taxa de Filtração Glomerular , Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , Infecções por HIV/virologia , Hepatite B/diagnóstico , Hepatite B/epidemiologia , Hepatite B/virologia , Hepatite C/diagnóstico , Hepatite C/epidemiologia , Hepatite C/virologia , Humanos , Rim/metabolismo , Rim/virologia , Fígado/metabolismo , Fígado/virologia , Cirrose Hepática/diagnóstico , Cirrose Hepática/epidemiologia , Cirrose Hepática/virologia , Testes de Função Hepática , Masculino , Pessoa de Meia-Idade , Prevalência , Insuficiência Renal Crônica/diagnóstico , Insuficiência Renal Crônica/epidemiologia , Insuficiência Renal Crônica/virologia
11.
Nat Commun ; 10(1): 3415, 2019 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-31363095

RESUMO

Conventional methods to discern adeno-associated virus (AAV) vector transduction patterns are based on high, stable expression of a reporter gene. As a consequence, conventionally described tropisms omit cell types that undergo transient transduction, or have low but undetectable levels of reporter expression. This creates a blind spot for AAV-based genome editing applications because only minimal transgene expression is required for activity. Here, we use editing-reporter mice to fill this void. Our approach sensitively captures both high and low transgene expression from AAV vectors. Using AAV8 and other serotypes, we demonstrate the superiority of the approach in a side-by-side comparison with traditional methods, demonstrate numerous, previously unknown sites of AAV targeting, and better predict the gene editing footprint after AAV-CRISPR delivery. We anticipate that this system, which captures the full spectrum of transduction patterns from AAV vectors in vivo, will be foundational to current and emerging AAV technologies.


Assuntos
Dependovirus/genética , Edição de Genes/métodos , Vetores Genéticos/genética , Transdução Genética , Animais , Sistemas CRISPR-Cas , Genes Reporter , Rim/virologia , Camundongos , Camundongos Endogâmicos C57BL , Baço/virologia
12.
Microb Pathog ; 135: 103617, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31283962

RESUMO

The bluegill sunfish, Lepomis macrochirus, is an important aquacultural and recreational species in southern China because of its excellent taste, rapid growth rate, and good looks. At present, few pathogens are known to affect the bluegill sunfish. However, an iridovirus-like disease recently caused heavy losses to the bluegill sunfish aquaculture industry in Guangdong, China. We report that a virus, designated BSMIV-SD-20171020, was isolated from diseased bluegill sunfish in China. The isolate was efficiently propagated in a Chinese perch brain (CPB) cell line. The cytopathic effect was observed, the MCP gene PCR amplified, and the virus observed with electron microscopy. Its viral titer in CPB cells reached 104.13 TCID50 mL-1. The mortality rate was 100% when bluegill sunfish were challenged with BSMIV-SD-20171020 at a dose of 103.13 TCID50/fish. A histopathological examination revealed basophilic hypertrophied cells in the intestine, liver, and spleen. A nucleotide sequence alignment and phylogenetic analysis of the major capsid protein revealed that isolate BSMIV-SD-20171020 is the species Infectious spleen and kidney necrosis virus (ISKNV), in the genus Megalocytivirus.


Assuntos
Infecções por Vírus de DNA/veterinária , Infecções por Vírus de DNA/virologia , Doenças dos Peixes/virologia , Iridoviridae/classificação , Iridoviridae/isolamento & purificação , Perciformes/virologia , Animais , Aquicultura , Encéfalo , Proteínas do Capsídeo/classificação , Proteínas do Capsídeo/genética , Linhagem Celular , China , Infecções por Vírus de DNA/patologia , Doenças dos Peixes/patologia , Peixes , Iridoviridae/genética , Iridoviridae/patogenicidade , Rim/patologia , Rim/virologia , Fígado/patologia , Fígado/virologia , Percas , Filogenia , Análise de Sequência de DNA/veterinária , Baço/patologia , Baço/virologia
13.
Vet Microbiol ; 235: 143-150, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31282372

RESUMO

Porcine parvovirus (PPV) is one of the major pathogens that bring about reproductive failure of pregnant sows. However, the study of the pathogenesis mechanism is circumscribed due to the lack of efficient genetic manipulation method. Infectious clone is a powerful tool for further studying the genetic mechanisms of PPV. In the present study, the gene fragment (157-4812) of PPV was amplified by PPV China isolate strain as a template, and PPV DNA fragments (1-182) forming Y-structure within in 5' end and (4788-5074) forming U-structure in 3' end were synthesized. And then, the above three fragments were inserted into plasmid pKQLL to congregate a PPV full-length recombinant plasmid by means of In-Fusion cloning technology. After the successful sequencing identification of the recombinant plasmid, the EcoR I restriction site was brought out as a genetic marker by nonsense mutation (A3058 T) to produce plasmid Y-PPV, which was transfected into PK-15 cells for rescue of virus. The rescued viral particles were observed under transmission electron microscopy, and the sequencing analysis showed that Y-PPV could stably carry the genetic marker. It could be seen that Y-PPV has similar replicate capability and pathogenicity as the wild-type parental PPV strain by cellular and animal experiments. These results confirmed that Y-PPV maintain similar biological characteristics with wild-type parental PPV strain. Infectious clone could be a valuable tool for studying the individual genes of PPV and applications in gene deletion or live vector vaccines.


Assuntos
Genoma Viral , Parvovirus Suíno/genética , Parvovirus Suíno/patogenicidade , Animais , Linhagem Celular , China , Clonagem Molecular , Marcadores Genéticos , Vetores Genéticos , Rim/citologia , Rim/virologia , Plasmídeos/genética , Suínos
14.
Transpl Infect Dis ; 21(5): e13146, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31306562

RESUMO

BACKGROUND: Transplanting kidneys from deceased donors with hepatitis C virus (HCV) viremia has been controversial for some time. Direct-acting antiviral agents have been shown to be highly effective in treating HCV infection. We report our experience with transplanting kidneys from HCV-positive donors with detectable viremia into HCV-negative recipients, followed by early treatment with a sofosbuvir-based antiviral regimen. METHODS: Data were collected from seven HCV-negative recipients receiving kidneys from five deceased HCV-viremic donors. Before transplantation, all intentional transplanted recipients had given informed consent regarding the acceptance of an HCV-viremic kidney. Recipients were closely monitored after transplant with measurements of HCV viremia, liver and renal function, and trough levels of immunosuppressive drugs. RESULTS: Four donors were infected with HCV genotype 1; the other with HCV genotype 3a. HCV viremia was detectable in all seven renal transplant recipients within 3 days after transplant. After determination of HCV genotype, antiviral treatment with a sofosbuvir-based regimen (sofosbuvir/ledipasvir, n = 4; sofosbuvir/velpatasvir, n = 3) was initiated within a median of 7 days after transplantation and was continued for 8 to 12 weeks. For all recipients, viral load was below the level of detection at the end of treatment, and all exhibited a sustained virologic response 12 weeks later. All recipients exhibited normal liver enzyme activity at the end of treatment. Renal allograft function and trough levels of tacrolimus remained stable. CONCLUSIONS: Early administration of a sofosbuvir-based regimen to HCV-negative recipients of kidneys from HCV-viremic donors is feasible and safe. The definition of an optimal therapeutic approach warrants further investigation.


Assuntos
Antivirais/administração & dosagem , Hepacivirus/efeitos dos fármacos , Hepatite C/prevenção & controle , Transplante de Rim/efeitos adversos , Rim/virologia , Sofosbuvir/administração & dosagem , Adulto , Esquema de Medicação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Resposta Viral Sustentada , Doadores de Tecidos , Transplantados , Carga Viral/efeitos dos fármacos , Viremia
15.
BMC Res Notes ; 12(1): 445, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31331365

RESUMO

OBJECTIVE: Tenofovir disoproxil fumarate (TDF) is a nucleotide analogue recommended in international HIV treatment guidelines. Purpose of this study was to estimate the long term effects of TDF on renal profile in a cohort of HIV patients in Ghana. Three hundred (300) consecutive HIV-positive patients who initiated TDF-based antiretroviral treatment in 2008 at the Korle-Bu Teaching Hospital were sampled. Creatinine clearance (CrCl) was calculated using the Cockcroft-Gault equation at baseline and renal impairment was defined as CrCl values of 30.0-49.9 mL/min (moderate renal impairment) and < 30 mL/min (severe renal impairment) as per institutional guidelines for renal function test. RESULTS: Median follow up time was 2.9 years (IQR 2.3-3.4 years). At study endpoint, 63 participants (21.0% [95% CI 6.5-26.1]) recorded CrCl rate below 50 mL/min indicating incident renal impairment, made up of 18.3% moderate renal impairment and 2.3% severe renal impairment. Factors associated with incidence of renal impairment were increasing age, decrease in creatinine clearance rate at baseline, WHO HIV stage III/IV and participants with BMI of < 18.5 kg/m2. Patients with identified renal impairment risk factors at ART initiation should be targeted and monitored effectively to prevent renal injury.


Assuntos
Infecções por HIV/tratamento farmacológico , Insuficiência Renal/diagnóstico , Tenofovir/uso terapêutico , Adulto , Fármacos Anti-HIV/efeitos adversos , Fármacos Anti-HIV/uso terapêutico , Estudos de Coortes , Feminino , Gana/epidemiologia , Infecções por HIV/epidemiologia , Humanos , Incidência , Rim/efeitos dos fármacos , Rim/fisiopatologia , Rim/virologia , Testes de Função Renal , Masculino , Pessoa de Meia-Idade , Insuficiência Renal/induzido quimicamente , Insuficiência Renal/fisiopatologia , Tenofovir/efeitos adversos
16.
Vet Microbiol ; 233: 133-139, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31176399

RESUMO

Feline morbillivirus (FeMV) is an emerging virus that was first described in Hong Kong in 2012. Several reports suggested the epidemiological association of FeMV infection with chronic kidney disease (CKD) in cats. The aim of this study was to investigate the presence and the genetic diversity of FeMV as well as the relationship between FeMV infection and CKD in cats from Northern Italy. Urine (n = 81) and kidney samples (n = 27) from 92 cats admitted to the Veterinary Teaching Hospital of the University of Milan between 2014 and 2017 were investigated for FeMV infection. FeMV RNA was detected in one urine sample (1.23%; 95% CI: 0.03-6.68%) and in two kidneys (7.40%; 95% CI: 0.91-24.28%). FeMV RNA was revealed only in urine or kidneys of cats without evidence of CKD. Phylogenetic analysis showed that the three strains clustered with FeMV strains retrieved from public database, forming a distinct sub-cluster of FeMV. The presence of distinct genotypes of FeMV found in this study is in accordance with previous studies demonstrating that FeMV strains are genetically diverse. A clear relationship between the presence of FeMV infection and CKD in the cats from Northern Italy was not observed, confirming recent reports that do not support the hypothesis that FeMV infection is associated with the development of CKD.


Assuntos
Doenças do Gato/epidemiologia , Doenças do Gato/urina , Rim/virologia , Infecções por Morbillivirus/veterinária , Morbillivirus/isolamento & purificação , Proteinúria/veterinária , Insuficiência Renal Crônica/veterinária , Animais , Gatos/virologia , Itália/epidemiologia , Morbillivirus/genética , Infecções por Morbillivirus/epidemiologia , Filogenia , Prevalência , Proteinúria/virologia , RNA Viral/genética , Insuficiência Renal Crônica/etiologia
17.
Vet Microbiol ; 233: 164-173, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31176404

RESUMO

Exosomes are small membrane-enclosed vesicles that participate in intercellular communication between cells. Numerous evidences suggested that exosomes derived from virus-infected cells can mediate virus transmission or/and regulate immune response. Foot-and-mouth disease virus (FMDV) is the prototype member of the Aphthovirus genus of the Picornaviridae family. It can cause highly infectious disease of cloven-hoofed livestock and significantly increase public awareness. However, the role of exosomes in the transmission of FMDV has still remained unknown. In this study, full length of FMDV genomic RNA and partial viral proteins were identified in purified exosomes isolated from FMDV-infected PK-15 cells with qRT-PCR and /MS. Exosomes from FMDV-infected cells were capable of transmitting infection to naive PK-15 cells and suckling mice. Furthermore, exosome-mediated infection cannot be fully blocked by FMDV-specific neutralizing antibodies. This finding highlights that FMDV transmission by exosomes as a potential immune evasion mechanism.


Assuntos
Exossomos/virologia , Vírus da Febre Aftosa/patogenicidade , Febre Aftosa/transmissão , Interações Hospedeiro-Patógeno , Evasão da Resposta Imune , Animais , Animais Recém-Nascidos , Anticorpos Neutralizantes , Exossomos/fisiologia , Vírus da Febre Aftosa/genética , Rim/citologia , Rim/virologia , Camundongos , Camundongos Endogâmicos C57BL , RNA Viral , Proteínas Virais/metabolismo
18.
Saudi J Kidney Dis Transpl ; 30(3): 560-563, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31249218

RESUMO

Rabies is an important neurological infection that is prevalent in tropical countries. The rabid animals can bring rabies to humans by biting. The disease can result in serious neurological problem and death is the end result. The best way is prevention of disease by postexposure prophylaxis against rabies. The effect of rabies on the renal system is little mentioned in the literature. In the previous literature, acute kidney injury was observable in half of the rabies patients. Rabies is also transmittable by organ transplantation. Although it is rare and <10 cases had ever been reported in literature, it is proven that kidney transplant patients are at risk of getting rabies if the donor come from endemic country or with a history of travel to endemic country and has unclear cause of death. Regarding rabies immunization, the use of vaccination for patients with the underlying renal failure is interesting. In this short article, the authors summarize on those important clinical issues of rabies and renal failure.


Assuntos
Lesão Renal Aguda/virologia , Rim/virologia , Vacinas Antirrábicas/administração & dosagem , Vírus da Raiva/patogenicidade , Raiva/prevenção & controle , Insuficiência Renal/virologia , Lesão Renal Aguda/epidemiologia , Animais , Humanos , Imunização , Transplante de Rim/efeitos adversos , Raiva/epidemiologia , Raiva/transmissão , Raiva/virologia , Vacinas Antirrábicas/efeitos adversos , Vírus da Raiva/imunologia , Insuficiência Renal/epidemiologia , Fatores de Risco , Doadores de Tecidos
19.
Int J Infect Dis ; 85: 54-56, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31129423

RESUMO

This case report describes the clinical findings of a 22-year-old pregnant woman with confirmed Zika virus infection, at 16 weeks of gestation, in Sucre, Colombia. Her ultrasound revealed severe oligohydramnios, intrauterine growth restriction, and a complete absence of the urinary bladder of the fetus. The poor prognosis led to the decision to terminate the pregnancy. Autopsy of the fetus revealed severe bilateral renal hypoplasia.


Assuntos
Retardo do Crescimento Fetal/virologia , Rim/anormalidades , Complicações Infecciosas na Gravidez/virologia , Bexiga Urinária/anormalidades , Infecção por Zika virus/virologia , Adulto , Colômbia , Feminino , Retardo do Crescimento Fetal/diagnóstico , Humanos , Rim/virologia , Gravidez , Bexiga Urinária/virologia , Adulto Jovem , Zika virus/fisiologia
20.
BMC Vet Res ; 15(1): 151, 2019 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-31101113

RESUMO

BACKGROUND: Caprine parainfluenza virus type 3 (CPIV3) is major pathogen of goat herds causing serious respiratory tract disease and economic losses to the goat industry in China. We analyzed the differential proteomics of CPIV3-infected Madin-Darby bovine kidney (MDBK) cells using quantitative iTRAQ coupled LC-MS/MS. In addition, four DEPs were validated by qRT-PCR and western blot analysis. RESULTS: Quantitative proteomics analysis revealed 163 differentially expressed proteins (DEPs) between CPIV3-infected and mock-infected groups (p-value < 0.05 and fold change > 1.2), among which 91 were down-regulated and 72 were up-regulated. Gene ontology (GO) analysis showed that these DEPs were involved in molecular functions, cellular components and biological processes. Biological functions in which the DEPs were involved in included diseases, genetic information processing, metabolism, environmental information processing, cellular processes, and organismal systems. STRING analysis revealed that four heat shock proteins (HSPs) included HSPA5, HSPA1B, HSP90B1 and HSPA6 may be associated with proliferation of CPIV3 in MDBK cells. qRT-PCR and western blot analysis showed that the selected HSPs were identical to the quantitative proteomics data. CONCLUSION: To our knowledge, this is the first report of the proteomic changes in MDBK cells after CPIV3 infection.


Assuntos
Proteínas de Choque Térmico/metabolismo , Proteômica , Infecções por Respirovirus/veterinária , Respirovirus/fisiologia , Animais , Western Blotting , Bovinos , Linhagem Celular , Cromatografia Líquida , Perfilação da Expressão Gênica , Proteínas de Choque Térmico/genética , Rim/virologia , Reação em Cadeia da Polimerase em Tempo Real , Respirovirus/genética , Infecções por Respirovirus/genética , Infecções por Respirovirus/metabolismo , Espectrometria de Massas em Tandem , Replicação Viral/fisiologia
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