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1.
Artigo em Inglês | MEDLINE | ID: mdl-33332262

RESUMO

A novel Gram-stain-negative, strictly aerobic, coccoid and agar-hydrolysing bacterium, designated RQJ05T, was isolated from the marine red algae Gelidium amansii collected from the coastal area of Rizhao, PR China. Cells of strain RQJ05T were approximately 0.8-1.0×1.3-3.0 µm in size and motile by means of a polar flagellum. Growth occurred at 4-33 °C (optimum, 25-30 °C), pH 7.0-8.5 (optimum, pH 7.5-8.0) and in the presence of 1.0-7.0 % (w/v) NaCl (optimum, 2.0-3.0 %). Strain RQJ05T showed oxidase-positive and catalase-negative activities. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain RQJ05T formed a phylogenetic lineage with members of the family Alteromonadaceae and exhibited 16S rRNA gene sequence similarities of 92.6, 91.3, 90.2 and 90.1 % to Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T, Paraphotobacterium marinum NSCS20N07DT and Algicola sagamiensis B-10-31T, respectively. The major cellular fatty acids of strain RQJ05T were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The major polar lipids of strain RQJ05T were phosphatidylethanolamine, phosphatidylglycerol and two aminophospholipids. Strain RQJ05T contained Q-8 as the major respiratory quinone. The genomic DNA G+C content was 39.0 mol%. On the basis of genotypic, phenotypic and phylogenetic evidence, strain RQJ05T is presented as a representative of a novel species in a new genus, for which the name Algibacillus agarilyticus gen. nov., sp. nov. is proposed. The type strain is RQJ05T (=KCTC 62846T=MCCC 1H00352T).


Assuntos
Alteromonadaceae/classificação , Filogenia , Rodófitas/microbiologia , Alteromonadaceae/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/química
2.
Int J Syst Evol Microbiol ; 70(9): 5048-5053, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32783808

RESUMO

A Gram-stain-negative, non-flagellated bacterium, designated ZY111T, was isolated from the surface of a marine red alga, which was collected from the coast in Weihai, Shandong Province, PR China. Strain ZY111T exhibited growth at 4-37 °C (optimum, 25-28 °C) in the presence of 0-8.0 % (w/v) NaCl (optimum, 2.0-4.0% NaCl) and at pH 6.5-9.5 (optimum, pH 7.0-8.0). The 16S rRNA gene sequence analysis revealed that strain ZY111T belonged to the genus Algibacter, with Algibacter amylolyticus DSM 29199T as its closest relative (97.7 % similarity). The averagenucleotide identity value of strain ZY111T with A. amylolyticus DSM 29199T was 79.03 %. The digitalDNA-DNA hybridization value of strain ZY111T with A. amylolyticus DSM 29199T was 22.40 %. The dominant fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH. The sole respiratory quinone was determined to be menaquinone-6. The polar lipid profile of strain ZY111T consisted of phosphatidylethanolamine, two unidentified aminolipids and three unidentified lipids. The G+C content was 31.9 mol%. The phenotypic, chemotaxonomic and phylogenetic data clearly showed that strain ZY111T represents a novel species of the genus Algibacter, for which the name Algibacter marinivivus sp. nov. is proposed. The type strain is ZY111T (=KCTC 62373T=MCCC 1H00295T).


Assuntos
Flavobacteriaceae/classificação , Filogenia , Rodófitas/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Flavobacteriaceae/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
3.
Int J Syst Evol Microbiol ; 70(6): 3679-3685, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32416740

RESUMO

A Gram-stain-negative, rod-shaped (0.2-0.3×1.0-2.4 µm), catalase-positive, oxidase-negative and non-motile bacterium, designated strain RZ26T, was isolated from the marine red algae collected from the coast of Weihai, PR China. Growth of strain RZ26T occurred at 15-33 °C (optimum, 25-28 °C), pH 6.0-9.5 (optimum, pH 7.0-7.5) and 0.5-5.0 % (w/v) NaCl (optimum, 2.0-3.0 %). Resuls of phylogenetic analysis based on 16S rRNA gene sequences showed that strain RZ26T was most closely related to Maribacter spongiicola DSM 25233T (96.2 % sequence similarity), followed by Maribacter forsetii DSM 18668T (96.1 %) and Maribacter vaceletii DSM 25230T (95.4 %). The average nucleotide identity and the average amino acid identity values between strain RZ26T and M. sedimenticola KCTC 12966T, M. spongiicola DSM 25233T, M. vaceletii DSM 25230T and M. forsetii DSM 18668T were 75.6, 76.2, 76.0, 76.7, 64.3, 63.9, 68.6 and 68.0 %, respectively. The digital DNA-DNAhybridization values based on the draft genomes between strain RZ26T and M. sedimenticola KCTC 12966T, M. spongiicola DSM 25233T and M. vaceletii DSM 25230T were 38.0, 35.1 and 37.1 %, respectively. The major fatty acids in strain RZ26T were iso-C17 : 0 3-OH, iso-C15 : 0 and C16 : 1 ω7c/C16 : 1 ω6c. The major respiratory quinone was MK-6. The dominant polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.0 mol%. Phylogenetic analysis shows strain RZ26T fell within a clade comprising species of the genus Maribacter. Polyphasic taxonomy indicates that the isolate represents a novel species of the genus Maribacter, for which the name Maribacter algarum sp. nov. is proposed, with type strain RZ26T (=KCTC 62992T=MCCC 1H00362T).


Assuntos
Flavobacteriaceae/classificação , Filogenia , Rodófitas/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Flavobacteriaceae/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
4.
Int J Syst Evol Microbiol ; 70(5): 3335-3339, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32375932

RESUMO

A Gram-stain-negative, non-spore-forming, aerobic, motile, curved rod-shaped bacterium, designed strain R148T was isolated from a coralline algae Tricleocarpa sp. collected from Weizhou island, PR China. The optimal growth of R148T occurred at 25 °C, pH 8-9 in the presence of 0.5 % (w/v) NaCl on the basis of amended marine broth 2216. The genomic DNA G+C content was 59.5 mol%. The only detected respiratory quinone was Q-10. The major polar lipids were phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and three unidentified ninhydrin-positive lipids. The major cellular fatty acids were C18 : 1ω7c, C16 : 1ω7c, C19 : 0cyclo 9, 10 DMA and C18 : 0. The results of 16S rRNA gene-based global alignment indicated that the closest neighbour of strain R148T was Pelagibius litoralis DSM 21314T (93.1 % similarity), the second is Limibacillus halophilus KCTC 42420T (92.2 %). The results of phylogenetic analysis indicated that R148T forms a distinct branch in the robust clade of R148T and P. litoralis DSM 21314T, while the taxonomic position of this clade in the family Rhodospirillaceae is ambiguous among phylogenetic approaches. The low 16S rRNA gene similarity and distinct polar lipid and cellular fatty acid profile could readily distinguish R148T from closely related type strains. So R148T is suggested to represent a novel species in a novel genus, for which the name Denitrobaculum tricleocarpae gen. nov., sp. nov. is proposed. The type strain is R148T (=MCCC 1K03781T=KCTC 72137T).


Assuntos
Filogenia , Rodófitas/microbiologia , Rhodospirillaceae/classificação , Ubiquinona/análogos & derivados , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Ilhas , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Rhodospirillaceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/química
5.
Int J Syst Evol Microbiol ; 70(5): 3427-3432, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32375976

RESUMO

A Gram-stain-negative, non-spore-forming, aerobic, curved rod-shaped bacterium, designed strain R142T, was isolated from a coralline algae Tricleocarpa sp. in the Beibu Gulf, China. Optimal growth occurred with 0-0.5 % (w/v) NaCl, at 25 °C and at pH 8. Global alignment based on 16S rRNA gene sequences indicated that strain R142T shared 93.8 % similarity with its closest type strain, Pseudomaricurvus alkylphenolicus KU14GT. Phylogenetic analyses showed that strain R142T forms a distinct branch alongside Maricurvus nonylphenolicus KU41ET, Pseudoteredinibacter isoporae SW-11T, Pseudomaricurvus alkylphenolicus KU14GT, Pseudomaricurvus alcaniphilus MEBiC06469T and Aestuariicella hydrocarbonica SM-6T. The major polar lipids of strain R142T were phosphatidylethanolamine and phosphatidylglycerol. The primary cellular fatty acids were C16 : 0, C16 : 1ω7c, C18 : 1ω7c, C18 : 0 and C14 : 0. The genome DNA G+C ratio was 56.4 mol%. The only detected respiratory quinone was ubiquinone 8. The low 16S rRNA gene sequence similarity and differences in cellular fatty acids readily distinguished strain R142T from all validly published type strains. Strain R142T is therefore suggested to represent a novel species of a new genus, for which the name Exilibacterium tricleocarpae gen. nov., sp. nov. is proposed. The type strain of Exilibacterium tricleocarpae is R142T (=MCCC 1K03816T=KCTC 72138T).


Assuntos
Gammaproteobacteria/classificação , Filogenia , Rodófitas/microbiologia , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Gammaproteobacteria/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/química
6.
Antonie Van Leeuwenhoek ; 113(3): 407-415, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31628626

RESUMO

A Gram-stain negative, non-motile, asporogenous, aerobic and rod-shaped bacterial strain, designated ZY113T, was isolated from the surface of a marine red alga collected from the coast in Weihai, Shandong Province, China. Strain ZY113T was found to grow at 4-37 °C (optimum at 28-30 °C), with 1.0-7.0% (w/v) NaCl (optimum 2.0-3.0%) and at pH 6.0-9.0 (optimum 7.0-8.0). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZY113T is a member of the genus Polaribacter, with Polaribacter dokdonensis KCTC 12392T as a close relative (97.4% similarity). The sole respiratory quinone was found to be menaquinone 6 (MK-6) and the major fatty acids were identified as iso-C15:0, iso-C15:0 3-OH and iso-C13:0. The polar lipids were found to consist of phosphatidylethanolamine, two unidentified aminolipids and three unidentified lipids. The G + C content of the genomic DNA was determined to be 30.1 mol%. On the basis of phenotypic, genotypic and phylogenetic analysis, strain ZY113T is considered to represent a novel species of the genus Polaribacter, for which the name Polaribacter aquimarinus sp. nov. is proposed. The type strain is ZY113T (= KCTC 62374T = MCCC 1H00296T).


Assuntos
Organismos Aquáticos , Flavobacteriaceae/classificação , Flavobacteriaceae/isolamento & purificação , Rodófitas/microbiologia , Técnicas de Tipagem Bacteriana , Flavobacteriaceae/genética , Genoma Bacteriano , Genômica/métodos , Genótipo , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
7.
Curr Microbiol ; 77(2): 294-299, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31776653

RESUMO

A Gram-stain-negative, strictly aerobic and moderately halophilic bacterium, designated strain AsT0115T, was isolated from a red marine alga, Asparagopsis taxiformis. Cells were non-motile rods showing oxidase-positive and catalase-negative activities. Growth was observed at 15-37 C (optimum, 25 C) and pH 6.5-7.5 (optimum, pH 7.0) and in the presence of 0-11.0% (w/v) NaCl (optimum, 2.0-3.0%). Strain AsT0115T contained iso-C15:0 and iso-C15:1 G as the predominant fatty acids. Menaquinone-6 was identified as the sole isoprenoid quinone. Major polar lipids consisted of phosphatidylethanolamine, an identified aminolipid and three unidentified polar lipids. The G+C content of the genomic DNA calculated from the whole genome sequence was 41.7 mol%. Strain AsT0115T was most closely related to Flagellimonas pacifica sw169T and Flagellimonas flava DSM 22638T with 96.5% and 95.6% 16S rRNA gene sequence similarities, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AsT0115T formed a phyletic lineage with F. pacifica sw169T within the genus Flagellimonas. Phenotypic, chemotaxonomic, and phylogenetic features clearly suggested that strain AsT0115T represents a novel species of the genus Flagellimonas, for which the name Flagellimonas algicola sp. nov. is proposed. The type strain is AsT0115T (= KACC 19790T = JCM 32942T).


Assuntos
Flavobacteriaceae/classificação , Flavobacteriaceae/genética , Filogenia , Rodófitas/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Flavobacteriaceae/química , Flavobacteriaceae/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
8.
J Antibiot (Tokyo) ; 73(2): 108-111, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31624337

RESUMO

The coculture of marine red algal-derived endophytic fungi Aspergillus terreus EN-539 and Paecilomyces lilacinus EN-531 induced the production of a new terrein derivative, namely asperterrein (1) and a known dihydroterrein (2), which were not detected in the axenic cultures of both strains. The production of the known secondary metabolites terrein (3), butyrolactone I (4), and dankasterone (6), derived from A. terreus EN-539, were depressed significantly in the coculture. Compounds 1-3 exhibited inhibitory activity against Alternaria brassicae, Escherichia coli, Physalospora piricola, and Staphylococcus aureus with MIC values ranging from 4 to 64 µg ml-1.


Assuntos
Anti-Infecciosos/farmacologia , Aspergillus/metabolismo , Ciclopentanos/farmacologia , Paecilomyces/metabolismo , Anti-Infecciosos/isolamento & purificação , Técnicas de Cocultura , Ciclopentanos/isolamento & purificação , Testes de Sensibilidade Microbiana , Rodófitas/microbiologia , Metabolismo Secundário
9.
Sci Rep ; 9(1): 12318, 2019 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-31444403

RESUMO

A chemical study of the EtOAc extract of Nemania bipapillata (AT-05), an endophytic fungus isolated from the marine red alga Asparagopsis taxiformis - Falkenbergia stage, led to the isolation of five new botryane sesquiterpenes, including the diastereomeric pair (+)-(2R,4S,5R,8S)-(1) and (+)-(2R,4R,5R,8S)-4-deacetyl-5-hydroxy-botryenalol (2), (+)-(2R,4S,5R,8R)-4-deacetyl-botryenalol (3), one pair of diastereomeric botryane norsesquiterpenes bearing an unprecedented degraded carbon skeleton, (+)-(2R,4R,8R)-(4) and (+)-(2R,4S,8S)-(5), which were named nemenonediol A and nemenonediol B, respectively, in addition to the known 4ß-acetoxy-9ß,10ß,15α-trihydroxyprobotrydial (6). Their structures were elucidated using 1D and 2D NMR, HRESIMS and comparison with literature data of similar known compounds. The absolute configurations of 2, 3 and 4 were deduced by comparison of experimental and calculated electronic circular dichroism (ECD) spectra, while those of 1 and 5 were assigned from vibrational circular dichroism (VCD) data. Compound 4 weakly inhibited acetylcholinesterase, whereas compound 1 inhibited both acetylcholinesterase and butyrylcholinesterase. Compounds 1, 3, 5 and 6 were tested against two carcinoma cell lines (MCF-7 and HCT-116), but showed no significant citotoxicity at tested concentrations (IC50 > 50 µM).


Assuntos
Endófitos/isolamento & purificação , Rodófitas/microbiologia , Terpenos/metabolismo , Xylariales/isolamento & purificação , Acetilcolinesterase/metabolismo , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Inibidores da Colinesterase/farmacologia , Células HCT116 , Humanos , Células MCF-7 , Espectroscopia de Prótons por Ressonância Magnética , Terpenos/química , Terpenos/isolamento & purificação
10.
Int J Syst Evol Microbiol ; 69(11): 3544-3550, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31433286

RESUMO

A Gram-stain-negative, strictly aerobic and moderately halophilic bacterium, designated strain Gri0909T, was isolated from a red marine alga, Grinnellia species, in the Yellow Sea of the Republic of Korea. Cells were motile by a single polar flagellum and short-rods showing oxidase and catalase activities. Growth was observed at 10-37 °C (optimum, 30 °C) and pH 6.0-9.0 (optimum, pH 8.0) and in the presence of 0-12.0 % (w/v) NaCl (optimum, 2.0-3.0 %). Strain Gri0909T contained C16 : 0, C18 : 1 2-OH, anteiso-C15 : 0 and summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c) as major fatty acids. Ubiquinone-10 was identified as the sole isoprenoid quinone. Major polar lipids consisted of phosphatidylethanolamine, an identified phospholipid, four unidentified aminolipids and five unidentified lipids. The G+C content of the genomic DNA calculated from the whole-genome sequence was 59.1 mol%. Strain Gri0909T was most closely related to Marivibriohalodurans ZC80T with 91.4 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Gri0909T formed a phyletic lineage within the family Rhodospirillaceae. The very low 16S rRNA gene sequence similarities, together with distinct phenotypic and chemotaxonomic properties, served to differentiate strain Gri0909T from phylogenetically closely related genera. Here it is proposed that strain Gri0909T represents a new species of a novel genus of the family Rhodospirillaceae, for which the name Hwanghaeella grinnelliae gen. nov., sp. nov. is proposed. The type strain is Gri0909T (=KACC 19793T=JCM 32943T).


Assuntos
Filogenia , Rodófitas/microbiologia , Rhodospirillaceae/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Rhodospirillaceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/química
11.
Int J Syst Evol Microbiol ; 69(8): 2514-2521, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31199221

RESUMO

Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method. Cells were Gram-stain-negative, strictly aerobic, non-flagellated, ovoid or rod-shaped and grew optimally at 20-25 °C, at pH 6-9 and with 2-4 % NaCl. Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources. The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0. The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid. Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria). Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas. The DNA G+C content of strain 1T was determined to be 43.9 mol%. Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively. The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp. nov. is proposed. The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).


Assuntos
Alteromonas/classificação , Carragenina/metabolismo , Filogenia , Rodófitas/microbiologia , Alteromonas/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hawaii , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas/química , Fosfatidilgliceróis/química , RNA Ribossômico 16S/genética , Alga Marinha/microbiologia , Análise de Sequência de DNA
12.
Int J Syst Evol Microbiol ; 69(7): 1967-1973, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31046898

RESUMO

A Gram-stain-negative, strictly aerobic bacterium, designated StC1T, was isolated from a marine alga, Stylonema cornu-cervi, in the Republic of Korea. Cells were oxidase- and catalase-positive rods that were motile by a single lateral flagellum. Growth of strain StC1T was observed at 30-45 °C(optimum, 37 °C), pH 6.0-11.0 (pH 7.0) and in the presence of 1.0-8.0 % (w/v) NaCl (2 %). Strain StC1T contained summed feature 8 (comprising C18 : 1ω7c/C18 : 1 ω6c) and 11-methyl-C18 : 1ω7c as the major fatty acids. Sulfoquinovosyl diacylglycerol, phosphatidylethanolamine and phosphatidylglycerol and ubiquinone-10 were identified as the major polar lipids and the sole isoprenoid quinone, respectively. The G+C content of the genomic DNA was 64.7 mol%. Strain StC1T was most closely related to Oricola cellulosilytica CC-AMH-OT, Nitratireductor basaltis J3T, Aquamicrobiumahrensii 905/1T and Mesorhizobium tamadayense Ala-3T with 97.3 , 96.9 , 96.8  and 96.6 % 16S rRNA gene sequence similarities, respectively, but it formed a distinct phylogenic lineage within the family Phyllobacteriaceae. On the basis of phenotypic, chemotaxonomic and molecular properties, strain StC1T represents a novel genus of the family Phyllobacteriaceae, for which the name Oceaniradius stylonematis gen. nov., sp. nov. is proposed. The type strain is StC1T (=KACC 19231T=JCM 32050T).


Assuntos
Phyllobacteriaceae/classificação , Filogenia , Rodófitas/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , Phyllobacteriaceae/isolamento & purificação , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/química
13.
Microbiologyopen ; 8(11): e00824, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31033207

RESUMO

Macroalgae-associated bacteria have already proved to be an interesting source of compounds with therapeutic potential. Accordingly, the main aim of this study was to characterize Asparagopsis armata-associated bacteria community and evaluate their capacity to produce substances with antitumor and antimicrobial potential. Bacteria were selected according to their phenotype and isolated by the streak plate technique. The identification was carried out by the RNA ribosomal 16s gene amplification through PCR techniques. The antimicrobial activities were evaluated against seven microorganisms (Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella enteritidis, Staphylococcus aureus, Saccharomyces cerevisiae, Candida albicans) by following their growth through spectrophotometric readings. Antitumor activities were evaluated in vitro on human cell lines derived from hepatocellular (HepG-2) and breast carcinoma (MCF-7) using the MTT method. The present work identified a total of 21 bacteria belonging to the genus Vibrio, Staphylococcus, Shewanella, Alteromonadaceae, Bacillus, Cobetia, and Photobacterium, with Vibrio being the most abundant (42.86%). The extract of Shewanella sp. ASP 26 bacterial strain induced the highest antimicrobial activity, namely against Bacillus subtilis and Staphylococcus aureus with an IC50 of 151.1 and 346.8 µg/mL, respectively. These bacteria (Shewanella sp.) were also the ones with highest antitumor potential, demonstrating antiproliferative activity on HepG-2 cells. Asparagopsis armata-associated bacteria revealed to be a potential source of compounds with antitumor and antibacterial activity.


Assuntos
Antibacterianos/metabolismo , Antineoplásicos/metabolismo , Bactérias/isolamento & purificação , Bactérias/metabolismo , Produtos Biológicos/metabolismo , Biota , Rodófitas/microbiologia , Bactérias/classificação , Técnicas de Tipagem Bacteriana , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Humanos , Testes de Sensibilidade Microbiana , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
14.
Mar Genomics ; 46: 8-15, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30852185

RESUMO

The Aquimarina genus is widely distributed throughout the marine environment, however little is understood regarding its ecological role, particularly when in association with eukaryotic hosts. Here, we examine the genomes of two opportunistic pathogens, Aquimarina sp. AD1 and BL5, and a non-pathogenic strain Aquimarina sp. AD10, that were isolated from diseased individuals of the red alga Delisea pulchra. Each strain encodes multiple genes for the degradation of marine carbohydrates and vitamin biosynthesis. These traits are hypothesised to promote nutrient exchange between the Aquimarina strains and their algal host, facilitating a close symbiotic relationship. Moreover, each strain harbours the necessary genes for the assembly of a Type 9 Secretion System (T9SS) and the associated gliding motility apparatus. In addition to these common features, pathogenic strains AD1 and BL5, encode genes for the production of flexirubin type pigments and a number of unique non-ribosomal peptide synthesis (NRPS) gene clusters, suggesting a role for these uncharacterised traits in virulence. This study provides valuable insight into the potential ecological role of Aquimarina in the marine environment and the complex factors driving pathogenesis and symbiosis in this genus.


Assuntos
Flavobacteriaceae/genética , Genômica , Interações Hospedeiro-Patógeno/genética , Microalgas/genética , Microalgas/microbiologia , Rodófitas/genética , Rodófitas/microbiologia , Flavobacteriaceae/patogenicidade , Simbiose
15.
Arch Microbiol ; 201(5): 705-712, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30810769

RESUMO

A group of five Gram-negative aerobic halophilic bacteria was isolated from the red alga Polysiphonia sp. specimen collected from the Sea of Japan seashore and subjected to a taxonomic study. On the basis of 16S rRNA gene sequence analysis, the novel isolates were affiliated to the genus Labrenzia sharing the highest gene sequence similarities of 98.1-98.4% with the type strain of Labrenzia suaedae KACC 13772T. The DNA-DNA hybridization values of 83-91% obtained between five novel strains, and 26 and 36% between two of the five novel strains and the closest neighbor Labrenzia suaedae KACC 13772T confirmed their assignment to the same separate species. Novel isolates were characterized by Q-10 as the major ubiquinone, by the predominance of C18:1ω7c followed by 11-methyl C18:1ω7c and C14:0 3-ОН in their fatty acid profiles. Polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, an unknown aminophospholipid, and an unknown phospholipid. Some of novel strains were found to inhibit growth of Gram-negative and Gram-positive test microorganisms. On the basis of phylogenetic analysis, DNA-DNA hybridization and phenotypic traits, a novel species with the name Labrenzia polysiphoniae sp. nov. (type strain KMM 9699T = rh46T = KACC 19711T), is proposed.


Assuntos
Antibacterianos/metabolismo , Antibiose/fisiologia , Rhodobacteraceae/classificação , Rhodobacteraceae/metabolismo , Rodófitas/microbiologia , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/análise , Japão , Hibridização de Ácido Nucleico , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Rhodobacteraceae/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimento , Ubiquinona/análise
17.
BMC Plant Biol ; 19(1): 37, 2019 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-30669983

RESUMO

BACKGROUND: Short chain oxylipins in plants as the main volatile organic carbon have been speculated to playing an important role for plant innate immunity, however, not yet intensively studied and far away established as the fully recognized algae defense signals. RESULTS: The production of 1-octen-3-ol is self-amplified via the fatty acid-oxylipin metabolic cycle through positive feedback loop. Production of 1-octen-3-ol may act as a messenger that induces P. haitanensis to be in a "primed" state and ready for defense by upregulating the synthesis of methyl jasmonic acid, indole-3-acetic acid, and gibberellin A3. Production of these oxylipins also adjust the redox state in cells, resulting in host defense activation. CONCLUSIONS: We provide the first demonstration that 1-octen-3-ol from P. haitanensis, can act as a self-stimulating community messenger. The multiple effects of 1-octen-3-ol may explain why P. haitanensis, a very ancient lineage within plant kingdom, thrives in the niche of intertidal zones.


Assuntos
Octanóis/metabolismo , Oxilipinas/metabolismo , Rodófitas/metabolismo , Transdução de Sinais , Bactérias/metabolismo , Ácidos Graxos não Esterificados/metabolismo , Expressão Gênica , Metabolismo dos Lipídeos , Lipoxigenase/genética , Lipoxigenase/metabolismo , Oxirredução , Fosfolipases/metabolismo , Rodófitas/genética , Rodófitas/imunologia , Rodófitas/microbiologia
18.
Arch Microbiol ; 201(4): 505-518, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30406296

RESUMO

The rich diversity of marine macroalgae and their associated bacterial flora represent a potential reservoir of bioactive compounds with valuable biotechnological and pharmaceutical use. Heterotrophic bacteria associated with the intertidal macroalgae were isolated and evaluated for their pharmacological properties using various in vitro models. Among 148 cultivable isolates, more than 50% were dominated by γ-Proteobacteria and Firmicutes, wherein 53 of them showed consistent antibacterial activity against a broad spectrum of clinically significant pathogens. The bacteria were characterized by extensive microbiological, molecular and chemical identification tools. The heterotrophs Bacillus amyloliquefaciens MTCC 12716 and Shewanella algae MTCC 12715 isolated from a red marine macroalga Hypnea valentiae exhibited potential anti-infective properties against multidrug-resistant pathogens, such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis (minimum inhibitory concentration of 6.25-12.5 µg/mL). The organic extract of B. amyloliquefaciens displayed significantly greater antioxidative properties (IC90 < 1 mg/mL) and the activities showed considerable positive correlation (r2 > 0.8, P < 0.05) with the inhibitory activities against angiotensin converting enzyme-I, pro-inflammatory cyclooxygenases and 5-lipoxygenase, dipeptidyl peptidase-4 and hydroxymethylglutaryl coenzyme A reductase, which were associated with hypertension, inflammation, diabetes, and hypercholesterolemia, respectively. The applications of nuclear magnetic resonance-based fingerprinting to analyze the characteristic signals in the solvent extracts and to correlate them with the pharmaceutical properties were underlined. The heterotrophic bacterium B. amyloliquefaciens MTCC 12716 might, therefore, serve as a potential therapeutic candidate to develop products with wide pharmaceutical applications.


Assuntos
Antibacterianos/farmacologia , Bactérias/química , Alga Marinha/microbiologia , Antibacterianos/química , Bactérias/isolamento & purificação , Processos Heterotróficos , Rodófitas/microbiologia
19.
Nat Prod Res ; 33(3): 443-446, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29577744

RESUMO

Endophytic fungi were isolated from red alga Asparagopsis taxiformis - Falkenbergia stage, collected from the Brazilian coast, and were identified as Annulohypoxylon stygium (AT-03) and A. yungensis (AT-06) based on their macro/micromorphological and molecular features. Bioassay-guided fractionation of the EtOAc extract from laboratory cultures of both strains yielded known compounds pyrogallol from A. stygium, (3 R)-scytalone and (3 R,4 R)-4-hydroxy-scytalone from A. yungensis. Pyrogallol was active against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli strains. An inactive fraction from A. stygium afforded two additional compounds, (3 R,4 R)-3,4,5-trihydroxy-1-tetralone and tyrosol. Optically active compounds had their stereochemistry determined by circular dichroism (CD) spectroscopy.


Assuntos
Endófitos/química , Fungos/química , Hidrocarbonetos Aromáticos/isolamento & purificação , Pirogalol/farmacologia , Rodófitas/microbiologia , Antibacterianos/química , Antibacterianos/isolamento & purificação , Brasil , Escherichia coli/efeitos dos fármacos , Hidrocarbonetos Aromáticos/química , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Naftóis/isolamento & purificação , Naftóis/farmacologia , Pirogalol/isolamento & purificação , Estereoisomerismo
20.
BMC Genomics ; 19(1): 842, 2018 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-30482156

RESUMO

BACKGROUND: Pyropia yezoensis is an important marine crop which, due to its high protein content, is widely used as a seafood in China. Unfortunately, red rot disease, caused by Pythium porphyrae, seriously damages P. yezoensis farms every year in China, Japan, and Korea. Proteomic methods are often used to study the interactions between hosts and pathogens. Therefore, an iTRAQ-based proteomic analysis was used to identify pathogen-responsive proteins following the artificial infection of P. yezoensis with P. porphyrae spores. RESULTS: A total of 762 differentially expressed proteins were identified, of which 378 were up-regulated and 384 were down-regulated following infection. A large amount of these proteins were involved in disease stress, carbohydrate metabolism, cell signaling, chaperone activity, photosynthesis, and energy metabolism, as annotated in the KEGG database. Overall, the data showed that P. yezoensis resists infection by inhibiting photosynthesis, and energy and carbohydrate metabolism pathways, as supported by changes in the expression levels of related proteins. The expression data are available via ProteomeXchange with the identifier PXD009363. CONCLUSIONS: The current data provide an overall summary of the red algae responses to pathogen infection. This study improves our understanding of infection resistance in P. yezoensis, and may help in increasing the breeding of P. porphyrae-infection tolerant macroalgae.


Assuntos
Proteínas de Algas/análise , Flavobacteriaceae/fisiologia , Doenças das Plantas/microbiologia , Proteômica/métodos , Rodófitas/metabolismo , Espectrometria de Massas em Tandem/métodos , Proteínas de Algas/metabolismo , Rodófitas/microbiologia
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