Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 967
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
J Agric Food Chem ; 68(8): 2426-2436, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32011134

RESUMO

Progressive degeneration of dopaminergic neurons in the substantia nigra is the characteristic feature of Parkinson's disease (PD) and the severity accelerates with aging. Therefore, improving dopamine level or dopamine receptor signaling is a standard approach for PD treatment. Herein, our results demonstrate that bromophenols 2,3,6-tribromo-4,5-dihydroxybenzyl alcohol (1), 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether (2), and bis-(2,3,6-tribromo-4,5-dihydroxybenzyl) ether (3) from red alga Symphyocladia latiuscula are moderate-selective human monoamine oxidase-A inhibitors and good dopamine D3/D4 receptor agonists. Bromophenol 3 showed a promising D4R agonist effect with a low micromole 50% effective concentration (EC50) value. All of the test ligands were docked against a three-dimensional (3D) model of hD3R and hD4R, and the result demonstrated strong binding through interaction with prime interacting residues-Asp110, Cys114, and His349 on hD3R and Asp115 and Cys119 on hD4R. Overall, the results demonstrated natural bromophenols, especially 1 and 3, from Symphyocladia latiuscula as multitarget ligands for neuroprotection, especially in PD and schizophrenia.


Assuntos
Inibidores da Monoaminoxidase/química , Monoaminoxidase/química , Doenças Neurodegenerativas/enzimologia , Fenóis/química , Extratos Vegetais/química , Receptores Dopaminérgicos/sangue , Rodófitas/química , Humanos , Estrutura Molecular , Monoaminoxidase/metabolismo , Doenças Neurodegenerativas/metabolismo , Receptores Dopaminérgicos/química , Receptores Dopaminérgicos/metabolismo
2.
Mar Drugs ; 17(10)2019 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-31590428

RESUMO

Keywords: griffithsin (GRFT); lectin; carbohydrate-binding; human immunodeficiency virus (HIV); microbicide; virus entry inhibitor.


Assuntos
Antivirais/farmacologia , Lectinas de Plantas/farmacologia , Rodófitas/química , Sequência de Aminoácidos , Animais , Infecções por HIV/tratamento farmacológico , Infecções por HIV/virologia , HIV-1/efeitos dos fármacos , Humanos
3.
Molecules ; 24(20)2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31618997

RESUMO

Obesity and diabetes are major metabolic disorders which are prevalent worldwide. Algae has played an important role in managing these disorders. In this study, Gracilaria edulis, a marine red algae, was investigated for antioxidant and hypoglycemic potential using in vitro models. De-polysaccharide methanol extract of G. edulis was sequentially partitioned with hexane, chloroform, ethyl acetate, and antioxidants, and hypoglycemic potentials were evaluated using multiple methods. High antioxidant potential was observed in the ethyl acetate fraction in terms of ferric reducing antioxidant power, iron chelating, and DPPH and ABTS radical scavenging activities, while the crude methanol extract exhibited potent oxygen radical-absorbance capacity. Potent α-amylase inhibitory activity was observed in the ethyl acetate fraction, while the ethyl acetate fraction was effective against α-glucosidase inhibition. Glucose diffusion was inhibited by the ethyl acetate fraction at 180 min, and the highest antiglycation activity was observed in both chloroform and ethyl acetate fractions. Additionally, gas chromatography-mass spectrometry analysis of the ethyl acetate fraction revealed the presence of several potent anti-diabetic compounds. In conclusion, G. edulis exhibited promising antidiabetic potential via multiple mechanisms. The ethyl acetate fraction exhibited the strongest hypoglycemic and antiglycation potential among the four fractions, and hence the isolation of active compounds is required to develop leads for new drugs to treat diabetes.


Assuntos
Antioxidantes/farmacologia , Hipoglicemiantes/farmacologia , Fenóis/farmacologia , Extratos Vegetais/farmacologia , Rodófitas/química , Antioxidantes/química , Cromatografia Gasosa-Espectrometria de Massas , Inibidores de Glicosídeo Hidrolases/química , Inibidores de Glicosídeo Hidrolases/farmacologia , Hipoglicemiantes/química , Fenóis/química , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/química
4.
Environ Pollut ; 255(Pt 2): 113291, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31600701

RESUMO

In the present investigation seaweeds of macroalgae like Kappaphycus alvarezii, Gracilaria salicornia and Gracilaria edulis used as novel biosorbent in native (KA, GS, GE) and ethanol modified (EKA, EGS, EGE) for Rhodamine B (RB) removal from aqueous solution in batch process. Effect of various biosorption parameters such as pH, initial concentration of RB, biosorbent dosage and contact time were studied. The maximum biosorption capacity determined as 9.84 (KA), 11.03 (GS), 8.96 (GE), 112.35 (EKA), 105.26 (EGS) and 97.08 mg/g (EGE), respectively towards the removal of RB from aqueous solutions. Better removal of RB was observed using EKA, EGS, and EGE biosorbents at 2.0 pH. The characterizations of the biosorbents were performed using Scanning Electron microscope and Fourier Transform Infrared Spectroscopy. Biosorption equilibrium data evaluated using Langmuir, Freundlich, Temkin, Dubinin-Radushkevich and Jovanovic isotherm model. The Langmuir isotherm model best suited the equilibrium data for all the biosorbents studied. The rate of RB removal subjected to kinetic analysis using pseudo-first-order, pseudo-second-order, intra-particle diffusion and Elovich models. Pseudo-second-order kinetic model better described the experimental data of the RB biosorption. Desorption studies performed using 0.1 M sodium hydroxide as eluting agents for regeneration and recycle analysis. The recyclability of the six biosorbents showed consistent biosorption capacity towards RB removal up to the entire three cycles. The studied biosorbents sourced from large volume and easily available, further biosorption performance indicated that the KA, GS, GE, EKA, EGS and EGE could be used as efficient, alternative and eco-friendly biosorbents for the removal of harmful dyes in the environment.


Assuntos
Gracilaria/química , Rodaminas/análise , Rodófitas/química , Alga Marinha/química , Poluentes Químicos da Água/análise , Purificação da Água/métodos , Adsorção , Concentração de Íons de Hidrogênio , Cinética , Modelos Teóricos , Termodinâmica
5.
Mar Drugs ; 17(9)2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31540318

RESUMO

This study investigated the anti-obesity effect of a polysaccharide-rich red algae Gelidium amansii hot-water extract (GHE) in high-fat (HF) diet-induced obese hamsters. GHE contained 68.54% water-soluble indigestible carbohydrate polymers. Hamsters were fed with a HF diet for 5 weeks to induce obesity, and then randomly divided into: HF group, HF with 3% guar gum diet group, HF with 3% GHE diet group, and HF with orlistat (200 mg/kg diet) group for 9 weeks. The increased weights of body, liver, and adipose in the HF group were significantly reversed by GHE supplementation. Lower plasma leptin, tumor necrosis factor-α, and interleukin-6 levels were observed in the GHE+HF group compared to the HF group. GHE also increased the lipolysis rate and decreased the lipoprotein lipase activity in adipose tissues. GHE induced an increase in the phosphorylation of AMP-activated protein kinase (AMPK) and the protein expressions of peroxisome proliferator-activated receptor alpha (PPARα) and uncoupling protein (UCP)-2 in the livers. The decreased triglyceride and total cholesterol in the plasma and liver were also observed in obese hamsters fed a diet with GHE. These results suggest that GHE exerts a down-regulation effect on hepatic lipid metabolism through AMPK phosphorylation and up-regulation of PPARα and UCP-2 in HF-induced obese hamsters.


Assuntos
Fármacos Antiobesidade/administração & dosagem , Suplementos Nutricionais , Obesidade/dietoterapia , Extratos Vegetais/administração & dosagem , Rodófitas/química , Adenilato Quinase/metabolismo , Animais , Fármacos Antiobesidade/química , Fármacos Antiobesidade/isolamento & purificação , Dieta Hiperlipídica/efeitos adversos , Modelos Animais de Doenças , Regulação para Baixo/efeitos dos fármacos , Humanos , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Mesocricetus , Obesidade/etiologia , Orlistate/administração & dosagem , Fosforilação/efeitos dos fármacos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Transdução de Sinais/efeitos dos fármacos , Proteína Desacopladora 2/metabolismo , Regulação para Cima/efeitos dos fármacos , Água/química
6.
Mar Drugs ; 17(9)2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31540326

RESUMO

Palmaria palmata is an edible red macroalga widely used for human consumption and valued for its high protein value. Despite its low total lipid content, it is rich in eicosapentaenoic acid (EPA). This seaweed has been scarcely explored with regard to its lipid composition. The polar lipids of seaweeds are nowadays recognized as important phytochemicals contributing to their add value valorization and providing support for claims of potential health benefits. The present study aimed to disclose the polar lipid profile of P. palmata, farmed in an integrated multi-trophic aquaculture (IMTA) through modern lipidomic approaches using high-resolution LC-MS and MS/MS and to screen for the antioxidant properties of this red macroalga. A total of 143 molecular species of lipids were identified, belonging to several classes of polar lipids, such as glycolipids, phospholipids, and betaine lipids. It is noteworthy that the most abundant lipid species in each class were esterified with eicosapentaenoic acid (EPA), accounting for more than 50% of the lipid content. The polar lipid extract rich in EPA showed antioxidant activity with an inhibition concentration (IC) of IC30 = 171 ± 19.8 µg/mL for α,α-diphenyl-ß-picrylhydrazyl radical (DPPH●) and IC50 = 26.2 ± 0.1 µg/mL for 2,20-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS●+). Overall, this study highlights that P. palmata farmed in an IMTA framework can be a sustainable source of beneficial lipids with antioxidant activity. Moreover, this red macroalga can be exploited for future applications as a source of lipids rich in EPA for food and feed, nutraceuticals, and cosmetics.


Assuntos
Ácido Eicosapentaenoico/análise , Depuradores de Radicais Livres/farmacocinética , Extratos Vegetais/farmacologia , Rodófitas/química , Alga Marinha/química , Aquicultura , Cromatografia Líquida de Alta Pressão , Cosméticos , Suplementos Nutricionais , Depuradores de Radicais Livres/análise , Depuradores de Radicais Livres/química , Alimento Funcional , Concentração Inibidora 50 , Extratos Vegetais/análise , Extratos Vegetais/química , Espectrometria de Massas em Tandem
7.
Mar Drugs ; 17(9)2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31527497

RESUMO

Macroalgae produce a wide range of monoterpenes as secondary metabolites of mevalonate (MVA) and/or methylerythritol phosphate (MEP) pathway (often including haloperoxidase action). Great biodiversity of macroalgal monoterpenes was reported including acyclic, monocyclic, and bicyclic structures. Halogenated monoterpenes exhibited significant biological activity (e.g., anticancer, antiplasmodial, and insecticidal) that is influenced by the number of present halogens (higher halogen content is preferable, especially bromine) and their position within the monoterpene skeleton. In distinction from the existing reviews, the present review provides novelty with respect to: (a) exclusively monoterpenes from red macroalgae are targeted; (b) biosynthesis, isolation, and analysis, as well as bioactivity of monoterpenes are represented; (c) the methods of their isolation, analysis, and structure elucidation are summarized; (d) the bioactivity of macroalgal monoterpenes is systematically presented with emphasis on anticancer activity; (e) the literature references were updated.


Assuntos
Antineoplásicos/farmacologia , Monoterpenos/farmacologia , Rodófitas/química , Alga Marinha/química , Animais , Antineoplásicos/análise , Antineoplásicos/química , Linhagem Celular Tumoral , Eritritol/análogos & derivados , Eritritol/metabolismo , Humanos , Ácido Mevalônico/metabolismo , Estrutura Molecular , Monoterpenos/análise , Monoterpenos/metabolismo , Rodófitas/metabolismo , Alga Marinha/metabolismo , Fosfatos Açúcares/metabolismo
8.
J Agric Food Chem ; 67(43): 11911-11921, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31475818

RESUMO

Red algae sulfated polysaccharides (RASP) were extracted from Porphyra haitanensis and Gracilaria lemaneiformis. RASP were applied to effervescent tablets to develop a type of functional food, termed red algae sulfated polysaccharide effervescent tablets (RASPET), based on the antiallergic activities of RASP. The antiallergic activities and the mechanisms of RASPET were investigated in an ovalbumin (OVA)-induced mouse model of food allergy. The results revealed that RASPET alleviated intestinal villi injury by scanning electron microscopy and anaphylactic symptoms; reduced OVA-specific immunoglobulin E, histamine, and mast cell protease-1 levels in the serum; reduced the level of serum interleukin-4; increased serum interferon-γ level; and decreased B cell and mast cell populations. Remarkably, RASPET increased the levels of serum interleukin-10, transforming growth factor-ß, and upregulated splenic CD4+foxp3+ T cell populations (15.28, 16.82, and 17.58%, respectively) compared to the OVA group (13.17%). In conclusion, RASPET attenuated OVA-induced anaphylaxis via the upregulation of regulatory T cells.


Assuntos
Anafilaxia/tratamento farmacológico , Antialérgicos/administração & dosagem , Ovalbumina/efeitos adversos , Polissacarídeos/administração & dosagem , Rodófitas/química , Linfócitos T Reguladores/imunologia , Anafilaxia/etiologia , Anafilaxia/imunologia , Animais , Antialérgicos/química , Modelos Animais de Doenças , Feminino , Histamina/imunologia , Humanos , Imunoglobulina E/imunologia , Interferon gama/imunologia , Interleucina-4/imunologia , Mastócitos/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Polissacarídeos/química , Comprimidos/administração & dosagem , Comprimidos/química
9.
Food Funct ; 10(9): 5697-5706, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31435629

RESUMO

Osteoarthritis (OA) is a common form of arthritis, which is characterized by the degeneration of articular cartilage, leading to joint dysfunction. Oral drug therapy seems to ameliorate some signs and symptoms of OA, but may be accompanied by side effects and does not appear to be effective long-term. Seaweed has received much attention for pharmacological application due to its various biomedical properties, including the anti-inflammation, antitumor, and antioxidant effects. This study investigated the ameliorative effects of a dietary polysaccharide from Eucheuma cottonii extract (ECE) on an anterior cruciate ligament transection with partial medial meniscectomy surgery (ACLT+MMx) to induce OA in high-fat diet (HFD)-induced obese rats. Male Sprague-Dawley rats were fed an HFD for 12 weeks before ACLT+MMx surgery, after which they were administered a daily oral gavage of saline (Sham, OB Sham, and OBOA) and either low-dose ECE (100 mg per kg body weight), high-dose ECE (400 mg per kg body weight), or glucosamine sulfate as a positive control (OBOAGS; 200 mg per kg body weight) for 5 weeks. Treatment with ECE decreased the body weight, triglyceride and total cholesterol (TC) levels, and the TC/high-density lipoprotein (HDL)-C ratio in the obese rats. Additionally, ECE downregulated the expression of proinflammatory cytokines, including tumor necrosis factor-α, interleukin-1ß, and leptin, and suppressed nuclear factor-kappa B and extracellular-signal-regulated kinase-1/2 expression, resulting in a decrease in the levels of matrix metalloproteinase (MMP)-1 and MMP-13 and prostaglandin-E2 and attenuated cartilage degradation. These results demonstrate that the dietary polysaccharide from ECE can suppress OA development in obese rats, suggesting its potential efficacy as a promising candidate for OA treatment.


Assuntos
Cartilagem Articular/efeitos dos fármacos , Citocinas/imunologia , Obesidade/complicações , Osteoartrite/tratamento farmacológico , Extratos Vegetais/administração & dosagem , Polissacarídeos/administração & dosagem , Rodófitas/química , Alga Marinha/química , Animais , Ligamento Cruzado Anterior/efeitos dos fármacos , Ligamento Cruzado Anterior/imunologia , Cartilagem Articular/imunologia , Citocinas/genética , Modelos Animais de Doenças , Humanos , Masculino , Obesidade/metabolismo , Osteoartrite/etiologia , Osteoartrite/genética , Osteoartrite/imunologia , Ratos , Ratos Sprague-Dawley
11.
Curr Pharm Des ; 25(11): 1172-1186, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31465278

RESUMO

BACKGROUND: With the advancement in the field of medical science, the idea of sustained release of the therapeutic agents in the patient's body has remained a major thrust for developing advanced drug delivery systems (DDSs). The critical requirement for fabricating these DDSs is to facilitate the delivery of their cargos in a spatio-temporal and pharmacokinetically-controlled manner. Albeit the synthetic polymer-based DDSs normally address the above-mentioned conditions, their potential cytotoxicity and high cost have ultimately constrained their success. Consequently, the utilization of natural polymers for the fabrication of tunable DDSs owing to their biocompatible, biodegradable, and non-toxic nature can be regarded as a significant stride in the field of drug delivery. Marine environment serves as an untapped resource of varied range of materials such as polysaccharides, which can easily be utilized for developing various DDSs. METHODS: Carrageenans are the sulfated polysaccharides that are extracted from the cell wall of red seaweeds. They exhibit an assimilation of various biological activities such as anti-thrombotic, anti-viral, anticancer, and immunomodulatory properties. The main aim of the presented review is threefold. The first one is to describe the unique physicochemical properties and structural composition of different types of carrageenans. The second is to illustrate the preparation methods of the different carrageenan-based macro- and micro-dimensional DDSs like hydrogels, microparticles, and microspheres respectively. Fabrication techniques of some advanced DDSs such as floating hydrogels, aerogels, and 3-D printed hydrogels have also been discussed in this review. Next, considerable attention has been paid to list down the recent applications of carrageenan-based polymeric architectures in the field of drug delivery. RESULTS: Presence of structural variations among the different carrageenan types helps in regulating their temperature and ion-dependent sol-to-gel transition behavior. The constraint of low mechanical strength of reversible gels can be easily eradicated using chemical crosslinking techniques. Carrageenan based-microdimesional DDSs (e.g. microspheres, microparticles) can be utilized for easy and controlled drug administration. Moreover, carrageenans can be fabricated as 3-D printed hydrogels, floating hydrogels, and aerogels for controlled drug delivery applications. CONCLUSION: In order to address the problems associated with many of the available DDSs, carrageenans are establishing their worth recently as potential drug carriers owing to their varied range of properties. Different architectures of carrageenans are currently being explored as advanced DDSs. In the near future, translation of carrageenan-based advanced DDSs in the clinical applications seems inevitable.


Assuntos
Carragenina/química , Sistemas de Liberação de Medicamentos , Polímeros , Rodófitas/química , Hidrogéis
12.
Curr Pharm Des ; 25(11): 1187-1199, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31465279

RESUMO

BACKGROUND: Recently, researchers have given more and more consideration to natural polysaccharides thanks to their huge properties such as stability, biodegradability and biocompatibility for food and therapeutics applications. METHODS: a number of enzymatic and chemical processes were performed to generate bioactive molecules, such as low molecular weight fractions and oligosaccharides derivatives from algal polysaccharides. RESULTS: These considerable characteristics allow algal polysaccharides and their derivatives such as low molecular weight polymers and oligosaccharides structures to have great potential to be used in lots of domains, such as pharmaceutics and agriculture etc. Conclusion: The present review describes the mains polysaccharides structures from Algae and focuses on the currents agricultural (fertilizer, bio-elicitor, stimulators, signaling molecules and activators) and pharmaceutical (wound dressing, tissues engineering and drugs delivery) applications by using polysaccharides and/or their oligosaccharides derivatives obtained by chemical, physical and enzymatic processes.


Assuntos
Clorófitas/química , Feófitas/química , Polissacarídeos/química , Polissacarídeos/farmacologia , Rodófitas/química , Agricultura , Sistemas de Liberação de Medicamentos , Fertilizantes , Polímeros , Engenharia Tecidual , Cicatrização
13.
Molecules ; 24(15)2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31366018

RESUMO

Catalytic conversion of actual biomass to valuable chemicals is a crucial issue in green chemistry. This review discusses on the recent approach in the levulinic acid (LA) formation from three prominent generations of biomasses. Our paper highlights the impact of the nature of different types of biomass and their complex structure and impurities, different groups of catalyst, solvents, and reaction system, and condition and all related pros and cons for this process.


Assuntos
Clorófitas/química , Produtos Agrícolas/química , Ácidos Levulínicos/síntese química , Lignina/química , Rodófitas/química , Biomassa , Catálise , Química Verde , Hidrólise , Monossacarídeos/química , Polissacarídeos/química , Solventes/química
14.
Curr Pharm Des ; 25(11): 1147-1162, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31258069

RESUMO

BACKGROUND: In recent decades, there has been an increased interest in the utilization of polysaccharides showing biological activity for various novel applications owing to their biocompatibility, biodegradability, non-toxicity, and some specific therapeutic activities. Increasing studies have started in the past few years to develop algal polysaccharides-based biomaterials for various applications. METHODS: Saccharide mapping or enzymatic profiling plays a role in quality control of polysaccharides. Whereby, in vitro and in vivo tests as well as toxicity level discriminating polysaccharides biological activities. Extraction and purification methods are performed in obtaining algal derived polysaccharides followed by chromatographic profiles of their active compounds, structural features, physicochemical properties, and reported biological activities. RESULTS: Marine algae are capable of synthesizing Glycosaminoglycans (GAGs) and non-GAGs or GAG mimetics such as sulfated glycans. The cell walls of algae are rich in sulfated polysaccharides, including alginate, carrageenan, ulvan and fucoidan. These biopolymers are widely used algal-derived polysaccharides for biological and biomedical applications due to their biocompatibility and availability. They constitute biochemical compounds that have multi-functionalization, therapeutic potential and immunomodulatory abilities, making them promising bioactive products and biomaterials with a wide range of biomedical applications. CONCLUSION: Algal-derived polysaccharides with clearly elucidated compositions/structures, identified cellular activities, as well as desirable physical properties have shown the potential that may create new opportunities. They could be maximally exploited to serve as therapeutic tools such as immunoregulatory agents or drug delivery vehicles. Hence, novel strategies could be applied to tailor multi-functionalization of the polysaccharides from algal species with vast biomedical application potentials.


Assuntos
Materiais Biocompatíveis , Clorófitas/química , Feófitas/química , Polissacarídeos/química , Rodófitas/química , Alginatos , Carragenina , Sistemas de Liberação de Medicamentos , Sulfatos
15.
Mar Drugs ; 17(8)2019 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-31349625

RESUMO

Breast cancer is the most common cancer type and a primary cause of cancer mortality among females worldwide. Here, we analyzed the anticancer efficacy of a novel bromochlorinated monoterpene, PPM1, a synthetic analogue of polyhalogenated monoterpenes from Plocamium red algae and structurally similar non-brominated monoterpenes. PPM1, but not the non-brominated monoterpenes, decreased selectively the viability of several triple-negative as well as triple-positive breast cancer cells with different p53 status without significantly affecting normal breast epithelial cells. PPM1 induced accumulation of triple-negative MDA-MB-231 cells with 4N DNA content characterized by decreased histone H3-S10/T3 phosphorylation indicating cell cycle arrest in the G2 phase. Western immunoblot analysis revealed that PPM1 treatment triggered an initial rapid activation of Aurora kinases A/B/C and p21Waf1/Cip1 accumulation, which was followed by accumulation of polyploid >4N cells. Flow cytometric analysis showed mitochondrial potential disruption, caspase 3/7 activation, phosphatidylserine externalization, reduction of the amount polyploid cells, and DNA fragmentation consistent with induction of apoptosis. Cell viability was partially restored by the pan-caspase inhibitor Z-VAD-FMK indicating caspase contribution. In vivo, PPM1 inhibited growth, proliferation, and induced apoptosis in MDA-MB-231 xenografted onto the chick chorioallantoic membrane. Hence, Plocamium polyhalogenated monoterpenes and synthetic analogues deserve further exploration as promising anticancer lead compounds.


Assuntos
Apoptose/efeitos dos fármacos , Neoplasias da Mama/tratamento farmacológico , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Monoterpenos/farmacologia , Antineoplásicos/farmacologia , Mama/efeitos dos fármacos , Mama/metabolismo , Neoplasias da Mama/metabolismo , Inibidores de Caspase/farmacologia , Caspases/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Feminino , Fase G2/efeitos dos fármacos , Histonas/metabolismo , Humanos , Células MCF-7 , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Plocamium/química , Rodófitas/química
16.
J Sci Food Agric ; 99(15): 6722-6730, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31350864

RESUMO

BACKGROUND: Pyropia yezoensis, rich in porphyran, is a medicine-edible red alga. In the present study, the physicochemical characteristics, conformational states and antitumor activities of a novel porphyran extracted from the high-yield algal strain Pyropia yezoensis Chonsoo2 and its two degraded derivatives by gamma irradiation were investigated. RESULTS: Pyropia yezoensis porphyran is a water-soluble, triple-helical sulfated hetero-galactopyranose, named PYP. PYP was degraded by gamma irradiation at 20 kGy and 50 kGy, giving two low molecular weight derivatives comprising PYP-20 and PYP-50, respectively. PYP with a higher molecular weight has a solution conformation different from PYP-20 and PYP-50. Three porphyrans had no toxicity in normal human liver cells (HL-7702) and showed antitumor effects on Hep3B, HeLa and MDA-MB-231. They had better antitumor against HeLa cells, exhibiting a similar inhibition ratio compared to 5-fluorouracil, with PYP especially exhibiting a higher inhibition ratio than 5-fluorouracil. With respect to HeLa cells, the different antitumor activities might be related to porphyran molecular weight and solution conformation. Furthermore, the HeLa cell cycle was blocked in the G2/M phase after PYP treatment, leading to cell proliferation inhibition. The induction of cell cycle arrest was related to the changes in the expression of p21, p53, Cyclin B1 and cyclin-dependent kinase 1. CONCLUSION: Pyropia yezoensis porphyran, as applied to medicine and functional food, could potentially be used as a non-toxic natural adjuvant in cancer therapy. © 2019 Society of Chemical Industry.


Assuntos
Antineoplásicos/farmacologia , Extratos Vegetais/farmacologia , Rodófitas/química , Sefarose/análogos & derivados , Antineoplásicos/isolamento & purificação , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ciclina B1/genética , Ciclina B1/metabolismo , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Extratos Vegetais/isolamento & purificação , Sefarose/isolamento & purificação , Sefarose/farmacologia , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo
17.
Mar Drugs ; 17(8)2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31357490

RESUMO

To date, a number of mannose-specific lectins have been isolated and characterized from seaweeds, especially from red algae. In fact, man-specific seaweed lectins consist of different structural scaffolds harboring a single or a few carbohydrate-binding sites which specifically recognize mannose-containing glycans. Depending on the structural scaffold, man-specific seaweed lectins belong to five distinct structurally-related lectin families, namely (1) the griffithsin lectin family (ß-prism I scaffold); (2) the Oscillatoria agardhii agglutinin homolog (OAAH) lectin family (ß-barrel scaffold); (3) the legume lectin-like lectin family (ß-sandwich scaffold); (4) the Galanthus nivalis agglutinin (GNA)-like lectin family (ß-prism II scaffold); and, (5) the MFP2-like lectin family (MFP2-like scaffold). Another algal lectin from Ulva pertusa, has been inferred to the methanol dehydrogenase related lectin family, because it displays a rather different GlcNAc-specificity. In spite of these structural discrepancies, all members from the five lectin families share a common ability to specifically recognize man-containing glycans and, especially, high-mannose type glycans. Because of their mannose-binding specificity, these lectins have been used as valuable tools for deciphering and characterizing the complex mannose-containing glycans from the glycocalyx covering both normal and transformed cells, and as diagnostic tools and therapeutic drugs that specifically recognize the altered high-mannose N-glycans occurring at the surface of various cancer cells. In addition to these anti-cancer properties, man-specific seaweed lectins have been widely used as potent human immunodeficiency virus (HIV-1)-inactivating proteins, due to their capacity to specifically interact with the envelope glycoprotein gp120 and prevent the virion infectivity of HIV-1 towards the host CD4+ T-lymphocyte cells in vitro.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Lectinas de Ligação a Manose/química , Lectinas de Ligação a Manose/farmacologia , Manose/química , Manose/farmacologia , Rodófitas/química , Sequência de Aminoácidos , Animais , Humanos
18.
J Microbiol Biotechnol ; 29(8): 1193-1203, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31280521

RESUMO

We investigated the protective effects of 3-bromo-4,5-dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey against hydrogen peroxide (H2O2)-induced apoptosis in Vero cells. BDB exhibited scavenging activity for DPPH, hydroxyl, and alkyl radicals. BDB also inhibited H2O2-induced lipid peroxidation, cell death, and apoptosis in Vero cells by inhibiting the production of ROS. To evaluate the molecular mechanisms of apoptosis inhibition, the expression of Bax/Bcl-xL and NF-κB was assessed by western blot assay. BDB significantly suppressed the cleavage of caspase-9 and PARP and reduced Bax levels in H2O2-induced Vero cells. Besides, BDB suppressed the phosphorylation of NF-κB and the translocation of p65 in H2O2-induced cells. Furthermore, we evaluated the effect of BDB on ROS production, cell death, and lipid peroxidation in an H2O2-stimulated zebrafish embryo model. Taken together, these results indicated that ROS generation and cell death were significantly inhibited by BDB in zebrafish embryos, thereby proving that BDB exerts excellent antioxidant activity in vitro and in vivo.


Assuntos
Benzaldeídos/farmacologia , Peróxido de Hidrogênio/efeitos adversos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Rodófitas/química , Animais , Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Caspase 9/metabolismo , Ciclo Celular/efeitos dos fármacos , Morte Celular/efeitos dos fármacos , Sobrevivência Celular , Feminino , Peroxidação de Lipídeos , Masculino , Modelos Animais , NF-kappa B/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Células Vero , Peixe-Zebra/anormalidades , Proteína X Associada a bcl-2/metabolismo , Proteína bcl-X/metabolismo
19.
Biocontrol Sci ; 24(2): 73-80, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31204358

RESUMO

Fungal infection mostly caused by marine oomycetes had hindered crustacean production thus searching for natural and safe treatment is currently needed. Thus, this study was conducted to investigate the antimycotic effect of different seaweed extract against marine oomycetes (Lagenidium spp. and Haliphthoros spp) . Two seaweeds species (Eucheuma cottonii and Caulerpa lentillifera) were extracted using ethanol, methanol and water. Each extracts was tested on four fungi strains of marine oomycetes species for minimum inhibitory concentration (MIC) and fungicidal activities. C. lentillifera ethanol extract showed the highest antifungal effect where it can inhibit three from four fungal strains. Meanwhile, E. cottonii ethanol extract has lowest MIC (500 ppm) and inhibit L. thermophilum IPMB 1401 and H. sabahensis IPMB 1402 hyphal growths. Antimycotic effect on zoospores production shows reduction in production after 12 h immersion for three marine oomycetes species. Seaweed extracts toxicity on Artemia sp. showed approximately 5% mortality at 12 h immersion. It is suggested that 12 h immersion of seaweed extract is a suitable treatment for marine oomycetes in aquaculture. This study does not only show potential alternative control method for crab larvae health management, it may also contribute to the sustainable development and food security of aquaculture industry.


Assuntos
Antifúngicos/farmacologia , Caulerpa/química , Oomicetos/efeitos dos fármacos , Extratos Vegetais/farmacologia , Rodófitas/química , Antifúngicos/isolamento & purificação , Testes de Sensibilidade Microbiana , Viabilidade Microbiana/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação
20.
J Agric Food Chem ; 67(26): 7297-7303, 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31244198

RESUMO

3,6-Anhydro-l-galactose (AHG) produced from agarose in red macroalgae was recently suggested as an anticariogenic sugar to replace widely used xylitol. However, the multi-step process for obtaining monomeric sugar AHG from agarose may be expensive. Generally, it is easier to obtain oligosaccharides than monosaccharides from polysaccharides. Therefore, a one-step process to obtain agarobiose (AB) from agarose was recently developed, and here, we suggest AB as a new anticariogenic agent, owing to its anticariogenic activity against Streptococcus mutans. Among AHG-containing oligosaccharides, AB, neoagarobiose (NAB), agarooligosaccharides (AOSs), and neoagarooligosaccharides (NAOSs), AB showed higher inhibitory activity than AOSs against the growth and lactic acid production of S. mutans; no such inhibitory activity was observed for NAB and NAOSs. This inhibitory effect of AB was comparable to the previously reported inhibitory activity of AHG against S. mutans. These results suggest that AB, which can be more economically and simply produced than AHG, may serve as an anticariogenic sugar.


Assuntos
Antibacterianos/farmacologia , Dissacarídeos/farmacologia , Oligossacarídeos/farmacologia , Extratos Vegetais/farmacologia , Rodófitas/química , Alga Marinha/química , Antibacterianos/química , Antibacterianos/isolamento & purificação , Dissacarídeos/química , Dissacarídeos/isolamento & purificação , Oligossacarídeos/química , Oligossacarídeos/isolamento & purificação , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Streptococcus mutans/efeitos dos fármacos , Streptococcus mutans/crescimento & desenvolvimento
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA