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1.
Afr Health Sci ; 19(2): 1858-1865, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31656468

RESUMO

Background: A significant proportion of newborns in the developing countries are born with congenital anomalies. Objective: This study investigated congenital infections due to Rubella virus, Toxoplasma gondii, Treponema pallidum among presumed normal neonates from full term pregnant women in Mwanza, Tanzania. Methods: Sera from mothers were tested for Treponema pallidum and Toxoplasma gondii infection while newborns from mothers with acute infections were tested for T. pallidum and T. gondii, and all newborns were tested for Rubella IgM antibodies. Results: A total of 13/300 (4.3 %) mothers had T. pallidum antibodies with 3 of them having acute infection. Two (0.7 %) of the newborns from mothers with acute infection were confirmed to have congenital syphilis. Regarding toxoplasmosis, 92/300 (30.7 %) mothers were IgG seropositive and 7 had borderline positivity, with only 1/99 (1%) being IgM seropositive who delivered IgM seronegative neonate. Only 1/300 (0.3 %) newborn had rubella IgM antibodies indicating congenital rubella infection. Conclusion: Based on these results, it is estimated that in Mwanza city in every 100,000 live births about 300 and 600 newborns have congenital rubella and syphilis infections, respectively. Rubella virus and T. pallidum are likely to be among common causes of congenital infections in developing countries.


Assuntos
Complicações Infecciosas na Gravidez/epidemiologia , Vírus da Rubéola/imunologia , Rubéola (Sarampo Alemão)/congênito , Rubéola (Sarampo Alemão)/epidemiologia , Sífilis Congênita/epidemiologia , Toxoplasma/imunologia , Toxoplasmose Congênita/epidemiologia , Treponema pallidum/imunologia , Adolescente , Adulto , Anticorpos Antivirais/sangue , Estudos Transversais , Feminino , Sangue Fetal/imunologia , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Recém-Nascido , Gravidez , Complicações Infecciosas na Gravidez/imunologia , Cuidado Pré-Natal , Prevalência , Rubéola (Sarampo Alemão)/diagnóstico , Vírus da Rubéola/isolamento & purificação , Estudos Soroepidemiológicos , Sífilis/complicações , Sífilis/epidemiologia , Sífilis Congênita/diagnóstico , Tanzânia/epidemiologia , Toxoplasma/isolamento & purificação , Toxoplasmose/complicações , Toxoplasmose/epidemiologia , Toxoplasmose Congênita/diagnóstico , Treponema pallidum/isolamento & purificação , População Urbana
2.
J Infect Chemother ; 25(10): 829-831, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31253475

RESUMO

Patients with measles or rubella infections manifest acute onset fever accompanying systemic exanthema, which are clinically difficult to be distinguish. Rapid diagnosis and differentiation of such epidemic viral diseases is essential to prevent outbreaks. We developed a single-tube multiplex real-time PCR assay for these indistinguishable viruses. We used previously-reported primer settings, with a slight modification of reporter dye, and applied to multiplex Taqman real-time PCR by cobas z480 (Roche Molecular Systems, Inc.). Consequently, the assay could detect 10 copies/10 µl of measles and rubella with coefficient of variations of 11.2% and 21.8%, respectively. Strengths of our methodology include simplicity of operation, short measurement time (2 h), uses of internal control (confirming a run of PCR), and quantitative measurement with high sensitivity. Both measles and rubella currently cause social outbreaks in Japan. We hope that our single-tube multiplex assay contributes to an early diagnosis, leading to an appropriate infection control measure and prevention of epidemics.


Assuntos
Sarampo/diagnóstico , Morbillivirus/isolamento & purificação , Vírus da Rubéola/isolamento & purificação , Rubéola (Sarampo Alemão)/diagnóstico , Surtos de Doenças/prevenção & controle , Estudos de Viabilidade , Humanos , Japão/epidemiologia , Sarampo/epidemiologia , Sarampo/virologia , Morbillivirus/genética , Reação em Cadeia da Polimerase Multiplex , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Rubéola (Sarampo Alemão)/epidemiologia , Rubéola (Sarampo Alemão)/virologia , Vírus da Rubéola/genética , Sensibilidade e Especificidade , Fatores de Tempo
3.
Invest Ophthalmol Vis Sci ; 60(7): 2399-2405, 2019 06 03.
Artigo em Inglês | MEDLINE | ID: mdl-31157832

RESUMO

Purpose: To investigate the clinical and virologic-associated and predictive factors of intraocular pressure (IOP) evolution over time and its severity in Fuchs' heterochromic iridocyclitis (FHC). Methods: Consecutive patients with both clinical FHC and intraocular synthesis of rubella virus (RV)-specific antibodies were included in this study. Specific ocular production of RV antibodies was confirmed using the quotient of serum/aqueous humor ratio of RV IgGs (Crv) and control antiviral IgGs (Cctl), using quantitative serology methods. Epidemiologic, clinical, biological, and virologic data at referral were collected and correlated with IOP values over time, occurrence, and severity of glaucoma. Results: Sixty-eight eyes of 68 patients were included. Mean age at diagnosis was 40.7 ± 11.1 years. Mean follow-up was 4.3 ± 4.3 years. Mean baseline Crv and Cctl values were 12.34 ± 14.67 and 216.70 ± 98.4, respectively. Mean baseline IOP was 17.2 ± 7.2 mm Hg (range, 9-40) and 15.6 ± 5.6 (range, 3-30) 5 years after referral. The predictive factors for pejorative IOP evolution over time and glaucoma severity were male sex (P = 0.03) and decreased Crv (P = 0.04) and presence of iris nodules (P < 0.001) and decreased Cctl (P = 0.02), respectively. Diagnostic delay was associated with increased likelihood of undergoing glaucoma surgery (P = 0.02). Conclusions: Time to diagnosis, male sex, presence of iris nodules at baseline, and decreased Crv and Cctl ratios were associated with increased likelihood of pejorative IOP evolution over time. Given the aggressiveness of glaucoma in FHC, these results provide interesting insight into what category of patients should need the closest screening.


Assuntos
Infecções Oculares Virais/diagnóstico , Glaucoma de Ângulo Aberto/diagnóstico , Pressão Intraocular/fisiologia , Iridociclite/diagnóstico , Vírus da Rubéola/imunologia , Rubéola (Sarampo Alemão)/diagnóstico , Adulto , Idoso , Anticorpos Antivirais/sangue , Anti-Hipertensivos/uso terapêutico , Humor Aquoso/virologia , Infecções Oculares Virais/imunologia , Infecções Oculares Virais/fisiopatologia , Feminino , Cirurgia Filtrante , Seguimentos , Glaucoma de Ângulo Aberto/fisiopatologia , Glaucoma de Ângulo Aberto/terapia , Humanos , Iridociclite/imunologia , Iridociclite/fisiopatologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Rubéola (Sarampo Alemão)/fisiopatologia , Rubéola (Sarampo Alemão)/terapia , Índice de Gravidade de Doença , Fatores de Tempo , Tonometria Ocular , Adulto Jovem
4.
BMC Infect Dis ; 19(1): 392, 2019 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-31068140

RESUMO

BACKGROUND: In Lao PDR, the epidemiology of varicella infection is uncertain, since it is not a notifiable disease and VZV outbreaks are rarely reported as fever/rash (F/R) diseases. METHODS: We estimated the seroprevalence of VZV (IgG ELISA) in different age cohorts (9 months to 46 years; N = 3139) and investigated VZV and 6 other viruses in patients during F/R outbreaks and in an ad hoc sentinel site in the context of the national reporting system (IgM ELISA, PCR). RESULTS: At least 80% of the sampled population had evidence of VZV infection before the age of 15. The largest increase in seroprevalence occurred between the age groups 1 to 5 and 6 to 7 year-olds. A VZV outbreak (clade 2) also occurred in this age group mostly during the first year of primary school (median age 6 years, interquartile range 4.0-7.5). During a dengue outbreak, 6% had varicella. At our F/R sentinel site, 14% of children with viral etiology were laboratory diagnosed as varicella and among others, a sizeable number of measles (N = 12) and rubella cases (N = 25) was detected compared to those reported for the whole country (N = 56 and 45), highlighting nationwide a large challenge of underreporting or misdiagnosis of these notifiable diseases because of lack of diagnostic laboratory capacity. CONCLUSION: We recommend strengthening the clinical and laboratory diagnosis of VZV, measles and rubella, the surveillance and reporting of notifiable F/R diseases by retraining of healthcare workers and by setting up sentinel sites and enhancing laboratory capacity.


Assuntos
Exantema/virologia , Febre/virologia , Infecção pelo Vírus da Varicela-Zoster/epidemiologia , Adolescente , Adulto , Anticorpos Antivirais/sangue , Varicela/diagnóstico , Varicela/epidemiologia , Criança , Pré-Escolar , Dengue/epidemiologia , Dengue/etiologia , Surtos de Doenças , Ensaio de Imunoadsorção Enzimática , Exantema/epidemiologia , Feminino , Febre/epidemiologia , Herpes Zoster/epidemiologia , Herpesvirus Humano 3/genética , Herpesvirus Humano 3/imunologia , Herpesvirus Humano 3/isolamento & purificação , Humanos , Lactente , Laos/epidemiologia , Masculino , Sarampo/diagnóstico , Sarampo/epidemiologia , Pessoa de Meia-Idade , Rubéola (Sarampo Alemão)/diagnóstico , Rubéola (Sarampo Alemão)/epidemiologia , Estudos Soroepidemiológicos , Infecção pelo Vírus da Varicela-Zoster/etiologia
5.
New Microbiol ; 42(2): 88-93, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30994178

RESUMO

This study compared the performance of the LIAISON®XL system of immunoglobulin (Ig) G and IgM immunoassays for the diagnosis of Toxoplasma gondii, cytomegalovirus (CMV), and rubella virus infections with that of the ARCHITECT system. Patient serum samples, previously screened and clinically diagnosed with T. gondii, CMV or rubella, were used to compare LIAISON®XL and ARCHITECT IgG and IgM immunoassays. LIAISON®XL Toxo and CMV IgG avidity assays were also compared with equivalent ARCHITECT assays and reference methods. Overall agreement between the LIAISON®XL and ARCHITECT assays was 99% and 92% for the Toxo IgG and IgM assays, respectively, 98% and 96% for the CMV IgG and IgM assays, respectively, and 93% and 98% for the rubella virus IgG and IgM assays, respectively. LIAISON®XL IgG Toxo and CMV avidity assays showed high concordance with the VIDAS® Toxo IgG avidity assay and an in-house CMV avidity assay (reference methods), and faster IgG avidity maturation in a larger number of samples collected months after the primary infection compared with equivalent ARCHITECT assays. LIAISON®XL assays for detection of anti-T. gondii, CMV and rubella virus IgG and IgM are at least equal to the competitor assays on the ARCHITECT platform.


Assuntos
Infecções por Citomegalovirus , Imunoensaio , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Rubéola (Sarampo Alemão) , Toxoplasmose , Infecções por Citomegalovirus/sangue , Infecções por Citomegalovirus/diagnóstico , Humanos , Imunoensaio/normas , Rubéola (Sarampo Alemão)/sangue , Rubéola (Sarampo Alemão)/diagnóstico , Toxoplasmose/sangue , Toxoplasmose/diagnóstico
6.
Int J Infect Dis ; 82: 7-8, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30825652

RESUMO

We report the case of a 32-year-old Japanese female who presented with a mild sore throat, rash and posterior cervical and posterior auricular lymphadenopathy. She was diagnosed with rubella. Japan has experienced multiple outbreaks of rubella in recent times. Travelers to Japan should consider getting vaccinated for rubella.


Assuntos
Linfadenopatia/diagnóstico , Rubéola (Sarampo Alemão)/diagnóstico , Adulto , Exantema/diagnóstico , Exantema/virologia , Feminino , Humanos , Japão , Linfadenopatia/virologia , Rubéola (Sarampo Alemão)/virologia
7.
Diagn Microbiol Infect Dis ; 94(3): 231-235, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30738692

RESUMO

This study evaluated the concordance of Architect™ chemiluminescent microparticle immunoassays with Captia™ ELISA for cytomegalovirus (CMV) IgM and IgG, with Enzygnost™ and Captia™ ELISA for rubella IgM and IgG and with Trep-Sure™ ELISA for syphilis treponemal antibodies in a mixed pediatric and obstetrical population. Total agreement between assays and Kappa statistic value were 82.5% (95% CI: 75.6-87.7) and 0.65 (95% CI: 0.54-0.77) for CMV IgM, 82.8% (95% CI: 76.7-87.6) and 0.65 (95% CI: 0.55-0.75) for CMV IgG, 89.2% (95% CI: 82.9-93.4) and 0.56 (95% CI: 0.36-0.75) for rubella IgM, 88.6% (95% CI: 82.9-92.6) and 0.74 (95% CI: 0.63-0.84) for rubella IgG, and 97.9% (95% CI: 94.5-99.4) and 0.89 (95% CI: 0.79-1.00) for syphilis treponemal antibodies. This study demonstrates that the Architect™ chemiluminescent microparticle immunoassays correlate well with other FDA-approved ELISA assays in this specific population.


Assuntos
Anticorpos Antibacterianos/sangue , Anticorpos Antivirais/sangue , Infecções por Citomegalovirus/diagnóstico , Testes Diagnósticos de Rotina/métodos , Técnicas Imunoenzimáticas/métodos , Rubéola (Sarampo Alemão)/diagnóstico , Sífilis/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Lactente , Recém-Nascido , Medições Luminescentes , Masculino , Pessoa de Meia-Idade , Adulto Jovem
8.
Eur J Clin Microbiol Infect Dis ; 38(5): 883-890, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30701339

RESUMO

Sampling the blood compartment by an invasive procedure such as phlebotomy is the most common approach used for diagnostic purposes. However, phlebotomy has several drawbacks including pain, vasovagal reactions, and anxiety. Therefore, alternative approaches should be tested to minimize patient's discomfort. Saliva is a reasonable compartment; when obtained, it generates little or no anxiety. We setup a multiplexed serology assay for detection of Toxoplasma gondii IgG and IgM, rubella IgG, and CMV IgG, in serum, whole blood, and saliva using novel plasmonic gold (pGOLD) chips. pGOLD test results in serum, whole blood, and saliva were compared with commercial kits test results in serum. One hundred twenty serum/saliva sets (Lyon) and 28 serum/whole blood/saliva sets (Nice) from France were tested. In serum and whole blood, sensitivity and specificity of multiplex T. gondii, CMV, and rubella IgG were 100% in pGOLD when compared to commercial test results in serum. In saliva, sensitivity and specificity for T. gondii and rubella IgG were 100%, and for CMV IgG, sensitivity and specificity were 92.9% and 100%, respectively, when compared to commercial test results in serum. We were also able to detect T. gondii IgM in saliva with sensitivity and specificity of 100% and 95.4%, respectively, when compared to serum test results. Serological testing by multiplex pGOLD assay for T. gondii, rubella, and CMV in saliva is reliable and likely to be more acceptable for systematic screening of pregnant women, newborn, and immunocompromised patients.


Assuntos
Infecções por Citomegalovirus/diagnóstico , Ouro/química , Análise Serial de Proteínas/normas , Rubéola (Sarampo Alemão)/diagnóstico , Saliva/imunologia , Testes Sorológicos/normas , Toxoplasmose/diagnóstico , Adolescente , Adulto , Anticorpos Antiprotozoários/análise , Anticorpos Antivirais/análise , Antígenos de Protozoários/química , Antígenos Virais/química , Criança , Pré-Escolar , Citomegalovirus/imunologia , Citomegalovirus/isolamento & purificação , Humanos , Imunoglobulina G/análise , Imunoglobulina M/análise , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Vírus da Rubéola/imunologia , Vírus da Rubéola/isolamento & purificação , Sensibilidade e Especificidade , Toxoplasma/imunologia , Toxoplasma/isolamento & purificação , Adulto Jovem
9.
Diagn Microbiol Infect Dis ; 94(3): 243-247, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30782464

RESUMO

Enzyme linked immunosorbent assay (ELISA) for antibody identification, is important for laboratory confirmation of rubella infection in different settings. The Enzygnost rubella ELISA, widely used in the World Health Organization (WHO) Global Measles and Rubella Laboratory Network, is expensive and often unavailable. Qualitative and quantitative performance of the Euroimmun ELISA was compared with the Enzygnost ELISA, for detection of rubella specific IgM, using 283 sera collected from suspected congenital rubella syndrome (CRS) patients and IgG antibodies using 435 sera from a serosurvey among pregnant women. Good qualitative agreement was observed for detection of both rubella specific IgM (94.7% agreement and κ of 0.86) and IgG (96.3% agreement and κ of 0.84). Bland-Altman analysis for IgG yielded a mean difference of 0.781 IU/ml with 97.1% values within ±2 SD of the mean difference. Our study findings suggest that Euroimmun ELISA may be considered for detection of rubella specific IgM in suspected CRS cases and rubella specific IgG in surveillance studies.


Assuntos
Anticorpos Antivirais/sangue , Testes Diagnósticos de Rotina/métodos , Ensaio de Imunoadsorção Enzimática/métodos , Complicações Infecciosas na Gravidez/diagnóstico , Kit de Reagentes para Diagnóstico , Vírus da Rubéola/imunologia , Rubéola (Sarampo Alemão)/diagnóstico , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Lactente , Recém-Nascido , Gravidez
10.
Indian Pediatr ; 56(1): 67-68, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30806367

RESUMO

BACKGROUND: Congenital B-cell Acute lymphoblastic leukemia (ALL) is a rare malignancy. CHARACTERISTICS: A newborn infant presented with purpuric spots and ecchymotic patches, blueberry muffin rash, depressed neonatal reflexes, respiratory distress and hepatosplenomegaly. Peripheral smear revealed atypical blast cells. Serum ELISA was positive for Rubella IgM and IgG antibodies. Flow cytometry suggested congenital B-cell ALL. OUTCOME: The baby died after 3 days due to suspected intracranial hemorrhage. MESSAGE: Congenital leukemia may be rarely associated with congenital rubella infection.


Assuntos
Leucemia-Linfoma Linfoblástico de Células Precursoras B , Rubéola (Sarampo Alemão) , Evolução Fatal , Feminino , Humanos , Recém-Nascido , Leucemia-Linfoma Linfoblástico de Células Precursoras B/complicações , Leucemia-Linfoma Linfoblástico de Células Precursoras B/diagnóstico , Púrpura/patologia , Rubéola (Sarampo Alemão)/complicações , Rubéola (Sarampo Alemão)/congênito , Rubéola (Sarampo Alemão)/diagnóstico , Rubéola (Sarampo Alemão)/patologia , Pele/patologia
12.
Microbiol Immunol ; 63(1): 32-35, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30549103

RESUMO

A total of 300 patients with nucleic acid test-confirmed rubella, mostly adults, were investigated to determine the clinical value of a rubella-specific IgM test using an EIA kit. IgM titers increased after rash onset, the median IgM titer being significantly higher 3 days post-onset than on previous days (P < 0.0001). Similarly, the IgM-positive rate at 3 days post-onset (61.5%) was significantly higher than on previous days (P < 0.0001). This IgM test against rubella at 3 days or more post-disease onset provides the clinically relevant information.


Assuntos
Técnicas de Laboratório Clínico/métodos , Técnicas Imunoenzimáticas/métodos , Imunoglobulina M/sangue , Rubéola (Sarampo Alemão)/diagnóstico , Rubéola (Sarampo Alemão)/imunologia , Adolescente , Adulto , Anticorpos Antivirais/análise , Criança , Pré-Escolar , Feminino , Humanos , Imunoglobulina G/sangue , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Vírus da Rubéola/imunologia , Soro/imunologia , Fatores de Tempo , Adulto Jovem
13.
Int Ophthalmol ; 39(2): 477-484, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29411259

RESUMO

PURPOSE: To report a case of choroidal neovascularization (CNV) associated with rubella retinopathy (RR) treated with intravitreal aflibercept. CASE PRESENTATION: A 15-year-old girl presented a complaint of visual decrease in her left eye. She had a history of hearing decrease since she was 1 year old in addition to patent ductus arteriosus. On ocular examination, the best corrected visual acuity (BCVA) was 20/20 in the right eye and 20/400 in the left eye. Dilated fundus examinations revealed a classic salt-and-pepper appearance in both eyes and a whitish subretinal lesion with retinal hemorrhages in the left macula. Fundus fluorescein angiography (FFA) of the left eye illustrated a pattern of diffuse spotty fluorescence with an active subfoveal CNV lesion, that hyperfluoresces in the early phases of the FFA, maintains well-demarcated borders, and leaks. Spectral domain optical coherence tomography (SD-OCT) revealed thickened and elevated retinal layers at the macula due to the subretinal and intraretinal fluid with foveal and extrafoveal protruding hyper-reflective lesion in the left eye. Single dose of intravitreal aflibercept was performed to the left eye and at the first month after the injection, the BCVA improved to 20/100 and the OCT revealed scar formation. At the follow-up visits, the macula was similar to those at the first month post-injection, and the BCVA was preserved. No additional injections were needed. CONCLUSION: Intravitreal aflibercept may be a treatment alternative, which provides satisfactory anatomical and functional results and leads to a better visual acuity in cases with RR complicated by CNV.


Assuntos
Neovascularização de Coroide/tratamento farmacológico , Infecções Oculares Virais/complicações , Receptores de Fatores de Crescimento do Endotélio Vascular/administração & dosagem , Proteínas Recombinantes de Fusão/administração & dosagem , Retinite/complicações , Rubéola (Sarampo Alemão)/complicações , Adolescente , Neovascularização de Coroide/diagnóstico , Neovascularização de Coroide/etiologia , Infecções Oculares Virais/diagnóstico , Feminino , Angiofluoresceinografia , Fundo de Olho , Humanos , Injeções Intravítreas , Receptores de Fatores de Crescimento do Endotélio Vascular/antagonistas & inibidores , Retinite/diagnóstico , Retinite/virologia , Rubéola (Sarampo Alemão)/diagnóstico , Rubéola (Sarampo Alemão)/virologia , Tomografia de Coerência Óptica
14.
Ocul Immunol Inflamm ; 27(3): 418-423, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-29370571

RESUMO

Purpose: To analyze intraocular cytokine levels and cell profiles in patients with rubella virus-associated uveitis (RVU). Methods: We collected intraocular fluid samples from patients with RVU (n = 10), uveitis of other causes (n = 27), and cataract (n = 22). Levels of 15 cytokines (IL-1ß, IL-1ra, IL-2, IL-6, IL-6rα, IL-7, IL-8, IL-10, IL-17A, IL-23, TARC, MCP-1, TNF-α, PlGF, and VEGF) were measured using multiplex assay, and intraocular cell populations were determined by multiparameter flowcytometry. Clinical characteristics of RVU patients were collected and compared to laboratory outcomes. Results: RVU patients exhibited high intraocular levels of MCP-1, IL-6rα, and TARC, whilst patients with noninfectious uveitis were characterized by high levels of PlGF. Cataract patients showed high levels of IL-2 and IL-23. Intraocular cell population of RVU patients disclosed mainly T-cells and monocytes/macrophages and B-cells were scarcely detected. Conclusion: RVU patients exhibit a cytokine profile distinct from noninfectious uveitis and cataract.


Assuntos
Humor Aquoso/metabolismo , Citocinas/metabolismo , Infecções Oculares Virais/metabolismo , Vírus da Rubéola/genética , Rubéola (Sarampo Alemão)/metabolismo , Uveíte/metabolismo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , DNA Viral/análise , Infecções Oculares Virais/diagnóstico , Infecções Oculares Virais/virologia , Feminino , Citometria de Fluxo , Humanos , Inflamação/metabolismo , Masculino , Pessoa de Meia-Idade , Rubéola (Sarampo Alemão)/diagnóstico , Rubéola (Sarampo Alemão)/virologia , Uveíte/diagnóstico , Uveíte/virologia , Adulto Jovem
16.
Int J Nanomedicine ; 13: 4795-4805, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30197515

RESUMO

Background: As nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools. Purpose: Antibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase (HRP) in conjugation of antigen. Method: Using a capture enzyme-linked immunosorbant assay for the determination of rubella virus. Results: Compared with antibody-conjugated HRP, the antigen-conjugated Au@Pt NRs are more stable and more robust, but less expensive. Based on the enhanced catalytic properties of this nanozyme probe, it was found that the antigen-conjugated Au@Pt NRs-based enzyme-linked immunosorbant assay exhibited good sensitivity. Conclusion: Our results indicate that these antigen-conjugated Au@Pt NRs represent a suitable nanozyme probe for future clinical applications under various conditions.


Assuntos
Antígenos Virais/imunologia , Ouro/química , Peroxidase do Rábano Silvestre/metabolismo , Nanotubos/química , Platina/química , Vírus da Rubéola/imunologia , Rubéola (Sarampo Alemão)/diagnóstico , Catálise , Humanos , Imunoensaio/métodos , Nanopartículas Metálicas/química , Rubéola (Sarampo Alemão)/imunologia , Rubéola (Sarampo Alemão)/virologia
17.
18.
Mymensingh Med J ; 27(3): 641-644, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30141457

RESUMO

TORCH {Toxoplasma gondii, Rubella, Cytomegalovirus (CMV), the Herpes Simplex Virus (HSV)} infection causes pregnancy wastage at any stage of gestation. Serological assays can be an important tool for diagnosis. It includes ELISA which is highly specific & sensitive with certain drawbacks like time consuming and for each genus separate test have to be done. Line-Immunoassay (LIA), a newer, cheaper and reliable technique on contrary is done on single panel strip. The present retrospective study was conducted with the aim to evaluate the efficacy of Line Immunoassay for detection of TORCH IgM antibodies. A total of 630 samples from the pregnant women with Bad Obstetric History (BOH) attending OPD and IPD of Obstetrics and Gynecology Department of Maharishi Markandeshwar Institute of Medical Science & Research (MMIMSR), Mullana, Ambala, Haryana, India from 2011 to 2015 were subjected to Line-Immunoassay and ELISA for detection of IgM antibodies against TORCH agents in Department of Microbiology. The analysis for TORCH complex showed 32.85% positivity by LIA while 36.03% by ELISA. The diagnostic accuracy of LIA when ELISA considered gold standard was also exhibited which showed Sensitivity of 90.75%, Specificity of 99.75% while PPV & NPV were 99.52%, 95.05% respectively. The statistical analysis showed results were statistically insignificant (p value >0.05). Comparison of detection of TORCH IgM antibodies by ELISA & Line-Immunoassay express good sensitivity & specificity requiring significantly less time and low cost by Line-Immunoassay, concludes that Line-Immunoassay may be used as screening test.


Assuntos
Imunoensaio , Imunoglobulina M , Complicações Infecciosas na Gravidez , Infecções por Citomegalovirus/diagnóstico , Feminino , Herpes Simples/diagnóstico , Humanos , Imunoensaio/métodos , Imunoglobulina M/análise , Índia , Gravidez , Complicações Infecciosas na Gravidez/diagnóstico , Estudos Retrospectivos , Rubéola (Sarampo Alemão)/diagnóstico , Toxoplasmose/diagnóstico
19.
Emerg Infect Dis ; 24(9): 1763-1765, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-30124420

RESUMO

Although rubella is epidemic in Indonesia, the phylogenetic profile of circulating rubella virus strains has not been clarified. In 2017, rubella virus was detected in 2 travelers who returned from Indonesia to Japan. These strains were classified into genotype 1E lineage 2, which may be an indigenous strain in Indonesia.


Assuntos
Vírus da Rubéola/isolamento & purificação , Rubéola (Sarampo Alemão)/diagnóstico , Viagem , Adulto , Diagnóstico Diferencial , Genótipo , Humanos , Indonésia , Japão , Masculino , Filogenia , Rubéola (Sarampo Alemão)/prevenção & controle , Rubéola (Sarampo Alemão)/virologia , Vírus da Rubéola/classificação , Vírus da Rubéola/genética
20.
J Glaucoma ; 27(7): e131-e132, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29847359

RESUMO

Intrauterine infections can affect various structures of the developing fetal eye. Rubella infection results in congenital cataracts, keratopathy, retinopathy and less commonly, glaucoma. Ophthalmic manifestations of intrauterine cytomegalovirus (CMV) infection have been reported to be chorioretinitis, optic nerve colobomas, and corneal opacities, but have not been implicated in congenital cataract or congenital glaucoma. Concurrent infection with both rubella and CMV virus has not been reported. We report concurrent rubella and CMV infection in a baby born with corneal opacification, severe congenital glaucoma, and congenital cataract. It is important to recognize these babies early and investigate for intrauterine infections rather than assume they are all primary congenital glaucoma. Involvement of the cornea, glaucoma, and cataract make management of these babies a major challenge requiring a multidisciplinary team approach.


Assuntos
Infecções por Citomegalovirus/complicações , Glaucoma/congênito , Glaucoma/diagnóstico , Glaucoma/microbiologia , Rubéola (Sarampo Alemão)/complicações , Opacidade da Córnea/congênito , Opacidade da Córnea/diagnóstico , Opacidade da Córnea/etiologia , Infecções por Citomegalovirus/congênito , Infecções por Citomegalovirus/diagnóstico , Humanos , Hidroftalmia/diagnóstico , Hidroftalmia/microbiologia , Recém-Nascido , Transmissão Vertical de Doença Infecciosa , Pressão Intraocular , Masculino , Rubéola (Sarampo Alemão)/congênito , Rubéola (Sarampo Alemão)/diagnóstico
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