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1.
Food Chem ; 300: 125169, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31336273

RESUMO

Red drupelet is a postharvest disorder of blackberries with several drupelets turning back to red. This affects visual quality and thus marketability and consumers' acceptance. However, the cause of this disorder as well as metabolite changes during color reversion have not been fully understood. Anthocyanins, cyanidin 3-glucoside, cyanidin 3-malonylglucoside, cyanidin 3-dioxalylglucoside, and total anthocyanin, were significantly lower in red drupelets than in black drupelets after 7 days of storage. Sugars and organic acids, lipids, and free amino acids also changed with storage and by color reversion. The untargeted metabolomics analyses indicated that red drupelets were generally differentiated from berries at harvest or black drupelets at metabolite level. The results of this study help better understand the red drupelet disorder. To our knowledge, this is the first study investigating red drupelet disorder by comparing black and red drupelets at metabolite level.


Assuntos
Metabolômica/métodos , Rubus/metabolismo , Aminoácidos/análise , Aminoácidos/metabolismo , Antocianinas/análise , Antocianinas/metabolismo , Cor , Qualidade dos Alimentos , Armazenamento de Alimentos , Frutas/química , Glucosídeos/análise , Glucosídeos/metabolismo , Lipídeos/análise , Rubus/química
2.
Plant Foods Hum Nutr ; 74(3): 399-404, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31273641

RESUMO

A simple and efficient ultrasonic-assisted extraction (UAE) technique was developed in order to find optimal conditions for the extraction of total phenolic compounds, flavonoids and anthocyanins in wild raspberry (Rubus idaeus L.) fruits. Several extraction variables, including methanol composition (v/v, %), solid-solvent ratio (g/mL), time (min) and extraction temperature (°C) were optimized using response surface methodology (RSM). Under optimal conditions for extraction, the total phenolics were found in the concentration of 383 mg GAE/100 g of fresh fruit weight, while HPLC-PDA analysis of the optimized extract showed the presence of cyanidin-3-glucoside, cyanidin-3-sophoroside, catechin, gallic and ellagic acid. The experimental values of DPPH and ABTS radical scavenging activities were 29.0 and 39.5 µmol Trolox/g of fresh fruit weight, respectively. In vitro simulated gastrointestinal digestion showed great raspberry phenolics stability. Our study assessed the bioaccessible phenolics in wild raspberry fruits and showed optimal conditions for the effective extraction of bioactive compounds for their analysis.


Assuntos
Flavonoides/análise , Frutas/química , Fenóis/isolamento & purificação , Compostos Fitoquímicos/isolamento & purificação , Rubus/química , Antocianinas/análise , Antocianinas/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Flavonoides/isolamento & purificação , Fenóis/análise , Compostos Fitoquímicos/análise , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Sonicação
3.
Food Chem ; 298: 125093, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260960

RESUMO

The purpose of this study was to investigate the impact of ozonation process on the level of oxidative stress markers in raspberries stored at room temperature. Raspberry fruit was ozonated with an ozone concentration of 8-10 ppm for 30 min, every 12 h, for 72 h of storage at room temperature. Research showed that ozonated raspberries were characterized by higher activity of superoxide dismutase, ascorbate peroxidase and phenylalanine ammonia-lyase. In turn, the ability to generate superoxide anion radical and hydrogen peroxide by ozone-treated fruit was significantly lower than in the control sample due to higher activities of ROS detoxification systems.


Assuntos
Armazenamento de Alimentos/métodos , Frutas/metabolismo , Estresse Oxidativo , Ozônio/química , Rubus/metabolismo , Antioxidantes/química , Antioxidantes/metabolismo , Ascorbato Peroxidases/metabolismo , Biomarcadores/química , Biomarcadores/metabolismo , Frutas/química , Peróxido de Hidrogênio/química , Peróxido de Hidrogênio/metabolismo , Fenilalanina Amônia-Liase/metabolismo , Espécies Reativas de Oxigênio/química , Espécies Reativas de Oxigênio/metabolismo , Rubus/química , Superóxido Dismutase/metabolismo , Superóxidos/química , Superóxidos/metabolismo , Temperatura Ambiente
4.
Food Chem ; 297: 125018, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31253265

RESUMO

Different Ohmic heating conditions (OH, 10, 100, and 1000 Hz at 25 V; 45, 60, and 80 V at 60 Hz) were assessed to manufacture whey-raspberry flavored beverages. The inhibition of α-glucosidase, α-amylase, and angiotensin-converting I enzymes, antioxidant capacity, fatty acid profile, and volatile organic compounds (VOCs) were determined. OH treated samples presented lower anthocyanins content than the conventional treatment (2.91 ±â€¯0.23 mg/g), while the mild-intermediate conditions (10,100-Hz at 25 V and 45,60-V at 60 Hz) presented the highest chemical antioxidant activity when compared to the extreme processing conditions (1000 Hz-25 V and 80 V-60 Hz). OH led to an increase of 10% in both α-glucosidase (>99%) and α-amylase (≥70%). Among the VOCs, furfural and 5-hydroxymethylfurfural, a major intermediate Maillard reaction product was found in all treatments. Overall, OH can be used in the processing of whey-flavored raspberry beverages.


Assuntos
Bebidas/análise , Aromatizantes/química , Rubus/química , Soro do Leite/química , Antocianinas/análise , Antioxidantes/química , Culinária , Ácidos Graxos/análise , Cromatografia Gasosa-Espectrometria de Massas , Glucosidases/antagonistas & inibidores , Glucosidases/metabolismo , Ondas de Rádio , Temperatura Ambiente , Compostos Orgânicos Voláteis/análise , alfa-Amilases/antagonistas & inibidores , alfa-Amilases/metabolismo
5.
J Agric Food Chem ; 67(25): 7025-7039, 2019 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-31240933

RESUMO

As a functional food, the unripe fruits of Rubus chingii Hu have been widely used in China for thousands of years. Twenty-five major ellagitannins (ETs) were identified from the unripe fruits, and a novel ellagitannin, chingiitannin A (1), together with four other known ETs (2-5) were isolated and identified by HPLC-QTOF-MS/MS and 2D-NMR. Chingiitannin A showed the highest α-glucosidase and α-amylase inhibitory activities (IC50 2.89 and 4.52 µM, respectively), which occurred in a reversible and noncompetitive manner. Static quenching was indicated in a fluorescence quenching assay. Molecular docking results revealed that chingiitannin A interacted with the enzymes mainly by hydrogen bonding and was bound in the allosteric site. Chingiitannin A was nontoxic, and it increased the glucose uptake in L6 myotubes. The results suggested that the unripe fruits of Rubus chingii Hu are rich sources of ETs, and chingiitannin A might be a good candidate for functional foods or antidiabetic drugs.


Assuntos
Inibidores de Glicosídeo Hidrolases/química , Taninos Hidrolisáveis/química , Hipoglicemiantes/química , Extratos Vegetais/química , Rubus/química , Animais , Linhagem Celular , Cromatografia Líquida de Alta Pressão , Frutas/química , Inibidores de Glicosídeo Hidrolases/farmacologia , Taninos Hidrolisáveis/farmacologia , Hipoglicemiantes/farmacologia , Cinética , Simulação de Acoplamento Molecular , Estrutura Molecular , Mioblastos/efeitos dos fármacos , Extratos Vegetais/farmacologia , Ratos , Espectrometria de Massas em Tandem , alfa-Glucosidases/química
6.
Food Chem ; 296: 160-166, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31202300

RESUMO

Vitamin E is a family of related compounds with different vitamin E activities and antioxidant properties that includes tocopherols, tocotrienols and plastochromanol-8. Plant oils could serve as an industrial source not only of tocopherols, but also tocotrienols and plastochromanol-8, which exhibit much stronger antioxidant activities than tocopherols. The aim of this study was a quantitative and qualitative analysis of vitamin E in certain plant oils. We demonstrated the presence of vitamin E derivatives in all the plant oils tested. The highest tocopherol contents were in pomegranate, wheat germ and raspberry seed oils. In general, γ-tocopherol was the predominant tocopherol homologue. Tocotrienols were also identified in most of the oils, but their content was much lower. The highest concentration of tocotrienols was in coriander seed oil. Plastochromanol-8 was present in most of the oils, but wheat germ oil was the richest source.


Assuntos
Óleos Vegetais/química , Vitamina E/análise , Antioxidantes/química , Cromatografia Líquida de Alta Pressão , Rubus/química , Rubus/metabolismo , Tocotrienóis/análise , gama-Tocoferol/análise
7.
J Agric Food Chem ; 67(26): 7526-7529, 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31184887

RESUMO

A limitation of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability (DRSA) due to the presence of pigments and colors in the extracts of plant-based foods was addressed. The pigments present in the test samples absorb in the same wavelength region as the DPPH radicals; this interferes with the elaborate absorbance readings of the DPPH radicals. In this contribution, electron paramagnetic resonance (EPR) spectroscopy for DPPH assay is proposed in order to avoid this limitation. In the EPR-DPPH assay, the underestimation of the radical scavenging abilities of four different pigment-containing extracts was corrected and this reflected up to a 16.1% change compared to the original values. Thus, the EPR-DPPH assay eliminated interference from the pigments coexisting in the reaction medium and improved the accuracy of the DPPH radical scavenging potential of such extracts. The principle of the proposed solution might also be employed in other assays which suffer from pigment interference.


Assuntos
Compostos de Bifenilo/química , Espectroscopia de Ressonância de Spin Eletrônica/métodos , Depuradores de Radicais Livres/química , Picratos/química , Pigmentos Biológicos/química , Extratos Vegetais/química , Beta vulgaris/química , Capsicum/química , Cor , Rubus/química
8.
BMC Complement Altern Med ; 19(1): 140, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31221152

RESUMO

BACKGROUND: Cholera is one of the most deadly diarrheal diseases that require new treatments. We investigated the neutralization of cholera toxin by five plant extracts obtained from the Rosaceae family that have been traditionally used in Poland to treat diarrhea (of unknown origin). METHODS: Hot water extracts were prepared from the dried plant materials and lyophilized before phytochemical analysis and assessment of antimicrobial activity using microdilution assays. The ability of the plant extracts to neutralize cholera toxin was analyzed by measurement of cAMP levels in cell cultures, enzyme-linked immunosorbent assay and electrophoresis, as well as flow cytometry and fluorescence microscopy studies of fluorescent-labeled cholera toxins with cultured human fibroblasts. RESULTS: The antimicrobial assays displayed modest bacteriostatic potentials. We found that the plant extracts modulate the effects of cholera toxin on intracellular cAMP levels. Three plant extracts (Agrimonia eupatoria L., Rubus fruticosus L., Fragaria vesca L.) suppressed the binding of subunit B of cholera toxin to the cell surface and immobilized ganglioside GM1 while two others (Rubus idaeus L., Rosa.canina L.) interfered with the toxin internalization process. CONCLUSIONS: The traditional application of the Rosaceae plant infusions for diarrhea appears relevant to cholera, slowing the growth of pathogenic bacteria and either inhibiting the binding of cholera toxin to receptors or blocking toxin internalization. The analyzed plant extracts are potential complements to standard antibiotic treatment and Oral Rehydration Therapy for the treatment of cholera.


Assuntos
Antibacterianos/farmacologia , Toxina da Cólera/toxicidade , Cólera/microbiologia , Extratos Vegetais/farmacologia , Rosaceae/química , Agrimonia/química , Antibacterianos/química , Linhagem Celular , Cólera/tratamento farmacológico , Cólera/metabolismo , Toxina da Cólera/metabolismo , Fragaria/química , Gangliosídeo G(M1)/metabolismo , Humanos , Extratos Vegetais/química , Rubus/química , Vibrio cholerae/efeitos dos fármacos , Vibrio cholerae/metabolismo
9.
J Agric Food Chem ; 67(22): 6177-6189, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-31083903

RESUMO

The aim of this work was to obtain phase II metabolites of cyanidin-3- O-glucoside and its aglycone using porcine liver enzymes. For this purpose, anthocyanins extracted from blackberry concentrate and containing mostly cyanidin-3- O-glucoside were incubated with the S9, microsomal, and cytosolic fractions of porcine liver. The reactions were targeted to the direction of the respective phase II transformation by the addition of activated cofactors. LC-MS n and LC-IMS-QTOF-MS analyses showed that one methylated, three glucuronidated and three sulfated metabolites of cyanidin-3- O-glucoside were generated. The aglycone, cyanidin, was sulfated and glucuronidated by the liver enzymes. In addition, both were glucuronidated and methylated simultaneously. The detected compounds and the generated data like exact masses, mass spectra, and CCS values may serve as a basis in the search for metabolites formed in vivo. As their effects are largely unexplored, the described synthesis may contribute to a better understanding of the metabolism of anthocyanins.


Assuntos
Antocianinas/síntese química , Glucosídeos/química , Microssomos Hepáticos/enzimologia , Extratos Vegetais/química , Rubus/química , Animais , Antocianinas/química , Biocatálise , Cromatografia Líquida de Alta Pressão , Frutas/química , Metilação , Microssomos Hepáticos/química , Estrutura Molecular , Suínos , Espectrometria de Massas em Tandem
10.
J Food Sci ; 84(6): 1371-1381, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31112298

RESUMO

Industrial processing of raspberries into juice and jam results in the production of with high content of lipophilic and hydrophilic phytochemicals. Usually considered as waste, raspberry pomace is occasionally cold-pressed to recover specialty oil. However, the resulting pomace press-cake (PPC) still contains 30% to 35% of lipophilic compounds, such as essential fatty acids, tocols, phytosterols, and ellagitannins initially present in pomace. In a perspective of sustainable development, we investigate an eco-friendly process using an aqueous enzyme-assisted extraction (AEAE) to simultaneously and effectively recover lipophilic compounds and polyphenols from the PPC. The performance of different combinations of carbohydrases and proteases was compared. After selecting the best enzymatic system, a definitive screening design involving six factors was then implemented to optimize the process. Under optimized conditions, 1.2 units of thermostable alkaline protease/100 g PPC, pH 9, 60 °C, and 2 hr hydrolysis, more than 38% of total PPC lipophilic content were recovered in the aqueous medium. The recovery of polyphenols and antioxidant activity was, respectively, 48% and 25% higher than obtained by extraction with methanol/acetone/water mixture. Such an AEAE extract might prove useful in food and nutraceutical applications. PRACTICAL APPLICATION: Raspberry pomace, a food industrial by-product, is often considered as waste. However, it is a rich source of phytochemicals, such as tocols, polyphenols, and polyunsaturated fatty acids. To overcome the drawbacks of organic solvent use, an enzyme-assisted extraction process was developed as an eco-friendly alternative to recover these bioactive compounds. Definitive screening design experimental design was used to enhance polyphenols and lipophilics extraction yields while reducing process costs. This extract is an oil-in-water emulsion, with high content in antioxidant phytochemicals, which might have potential for use in nutraceutical applications. Therefore, this green process developed for the valorization of raspberry pomace is considered as a perspective of sustainable development.


Assuntos
Antioxidantes/análise , Proteínas de Bactérias , Endopeptidases , Frutas/química , Química Verde , Compostos Fitoquímicos/análise , Extratos Vegetais/química , Rubus/química , Antioxidantes/farmacologia , Emulsões , Ácidos Graxos Essenciais/análise , Indústria Alimentícia , Humanos , Taninos Hidrolisáveis/análise , Taninos Hidrolisáveis/farmacologia , Resíduos Industriais , Compostos Fitoquímicos/farmacologia , Fitosteróis/análise , Extratos Vegetais/farmacologia , Polifenóis/análise , Polifenóis/farmacologia , Tocoferóis/análise
11.
J Food Sci ; 84(6): 1522-1528, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31120586

RESUMO

Berries are among the nutritionally richest fruits and the mixing of these fruits can originate products with peculiar characteristics and added value, since the fruit mixture allows adding nutritional and sensory value to the final product. Thus, in this study we aimed to optimize a mixed berry jelly formulation of red fruit (blackberry, blueberry, and strawberry), through the mixture design, desirability function, and response surface methodology. The fruit mixture influenced the physical-chemical, sensory, and nutritional characteristics of mixed jelly, and it was observed that the mixture of two or more fruits may increase the acceptance of the product and even its nutritional value. According to the contour curve graph for the sensory and nutritional desirability function, the ideal mixed berry jelly should contain 55% to 100% blackberry, 0% to 20% blueberry, and 0% to 40% strawberry. The exact proportion of fruit may depend on seasonality or the commercial value of the fruit. PRACTICAL APPLICATION: In Brazil, there are many blackberry, blueberry, and strawberry cultivars grown in subtropical regions and high perishability hinders the fruit consumption in fresh form. In this context, through this study it was possible to verify which fruits are more suitable for processing in the form of jelly, as well as to optimize, based on sensory and nutritional characteristics, a mixed berry jelly formulation with greater attractiveness to consumers.


Assuntos
Mirtilos Azuis (Planta)/química , Manipulação de Alimentos/métodos , Fragaria/química , Frutas/química , Rubus/química , Brasil , Humanos , Valor Nutritivo , Paladar
12.
Phytochemistry ; 164: 122-129, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31125862

RESUMO

A phytochemical study on the fruits of Rubus idaeus L. (Rosaceae) yielded eight pairs of enantiomeric lignans, including one undescribed furolactone named (-)-idaeusinol A and six undescribed furofuran derivatives named (+/-)-idaeusinol B-D. The structures of these isolated compounds were elucidated by spectroscopic analyses and a combination of computational techniques including gauge-independent atomic orbital (GIAO) calculation of 1D NMR data and TD-DFT calculation of electronic circular dichroism (ECD) spectra. Bioactivity screenings suggested that (+)-idaeusinol D exhibited the most significant protective effect against H2O2-induced neurotoxicity at the concentration of 25 µM. In contrast, (-)-idaeusinol D, as the enantiomer of (+)-idaeusinol D, showed no effect against H2O2-induced neurotoxicity at both 25 and 50 µM concentration.


Assuntos
Furanos/farmacologia , Lactonas/farmacologia , Fármacos Neuroprotetores/farmacologia , Rubus/química , Sobrevivência Celular/efeitos dos fármacos , Teoria da Densidade Funcional , Relação Dose-Resposta a Droga , Furanos/química , Furanos/isolamento & purificação , Humanos , Peróxido de Hidrogênio/antagonistas & inibidores , Peróxido de Hidrogênio/farmacologia , Lactonas/química , Lactonas/isolamento & purificação , Conformação Molecular , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/isolamento & purificação , Estereoisomerismo , Relação Estrutura-Atividade , Células Tumorais Cultivadas
13.
BMC Complement Altern Med ; 19(1): 89, 2019 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-31023273

RESUMO

BACKGROUND: Rubus coreanus fruit (RF) has been used as a traditional medicine formulation to treat various diseases including diarrhea, asthma, and cancer in East Asia (Korea, China, and Japan). RF, which is native to Korea, has a larger fruit size than that of exotic species. In this study, we aimed to compare the anti-inflammatory activities of immature and mature RF extracted with different solvents. METHODS: Mature and immature RF (MRF and IRF) were extracted with 30% ethanol, 70% ethanol and water at room temperature. The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Anti-inflammatory activity was determined by measuring nitric oxide (NO) production, expression of inflammatory proteins (inducible NO synthase [iNOS], cyclooxygenase [COX]-2, nuclear factor [NF]-κB, and inhibitor of NF-κB [IκB]), and inflammatory cytokines using polymerase chain reaction in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. RESULTS: The IRF 30% ethanol extract showed higher radical scavenging activity in DPPH and ABTS assays (half-maximal inhibitory concentration [IC50] 16.0 ± 0.5 and 15.9 ± 0.4) than MRF did. In addition, the IRF 30% ethanol extract (200 µg/mL) significantly reduced the production of the inflammatory mediator NO by approximately 80% and inhibited iNOS, COX-2, phosphorylated (p)-IκB, and p-NF-κB activation compared with MRF. Moreover, IRF extract decreased the inflammatory cytokines tumor necrosis factor-α, interleukin (IL)-1ß, and IL-6 compared with the MRF extract. CONCLUSIONS: This study revealed that IRF showed more beneficial effects than MRF did in LPS-stimulated RAW 264.7 macrophages, suggesting that IRF may be a useful anti-inflammatory agent.


Assuntos
Anti-Inflamatórios/farmacologia , NF-kappa B/metabolismo , Extratos Vegetais/farmacologia , Rubus/química , Transdução de Sinais/efeitos dos fármacos , Animais , Sobrevivência Celular/efeitos dos fármacos , Citocinas/metabolismo , Depuradores de Radicais Livres , Frutas/química , Lipopolissacarídeos/efeitos adversos , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Extratos Vegetais/química , Células RAW 264.7
14.
Int J Biol Macromol ; 132: 109-118, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30926502

RESUMO

A native polysaccharide (RCPI) with sugar content of 79.94 ±â€¯0.57% was isolated from raspberry fruits. A degraded polysaccharide (DRCPI) with sugar content of 83.82 ±â€¯0.71% was obtained from the crude polysaccharides after degradation by Fe2+-H2O2 treatment. The molecular weights of RCPI and DRCPI were determined to be 411,000 and 8010 Da, respectively. GC analysis revealed that both RCPI and DRCPI were composed of the same monosaccharides (rhamnose, arabinose, xylose, mannose, glucose, galactose, and galacturonic acid) in different molar ratios. IR and NMR spectroscopy confirmed that RCPI and DRCPI possessed similar structural characteristics, although DRCPI displayed higher antioxidant activities and greater thermal stability. Furthermore, DRCPI exhibited excellent rheological properties. These data provide evidence to support the application of raspberry-derived polysaccharides in functional foods.


Assuntos
Frutas/química , Polissacarídeos/química , Polissacarídeos/farmacologia , Reologia , Rubus/química , Temperatura Ambiente , Antioxidantes/química , Antioxidantes/farmacologia , Dano ao DNA , Peroxidação de Lipídeos/efeitos dos fármacos , Peso Molecular , Monossacarídeos/análise , alfa-Amilases/antagonistas & inibidores
15.
Food Chem Toxicol ; 127: 237-250, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30914354

RESUMO

In the present study, the biological properties, including, the enzyme inhibitory and antioxidant activities, as well as, the phytochemical profile of the ethyl acetate, methanol, and water extracts of Rubus sanctus Schreb. and Rubus ibericus Juz. leaves were determined using in vitro bioassays. Wide range of phytochemicals, including, hydroxybenzoic acids, hydroxycinnamic acids, acylquinic acids, ellagitannins, flavonoids, and triterpenoid saponins were determined using UHPLC-ESI/HRMS technique. The ethyl acetate and methanol extracts of the studied Rubus species effectively inhibited acetyl and butyryl cholinesterase. On the other hand, R. sanctus water extract showed low inhibition against α-amylase and prominent inhibitory action against α-glucosidase. Data collected from this study reported the radical scavenging and reducing potential of the studied Rubus species. Investigation of the protective effects of the different extracts of R. sanctus and R. ibericus in experimental model of ulcerative colitis was performed. The extracts were also tested on spontaneous migration of human colon cancer cells (HCT116) in wound healing experimental paradigm. Only R. sanctus methanol extract inhibited spontaneous HCT116 migration in the wound healing test. Our results suggested that R. sanctus and R. ibericus may be potential candidates as sources of biologically-active compounds for the development of nutraceuticals, pharmaceuticals, and/or cosmetics.


Assuntos
Compostos Fitoquímicos/análise , Rubus/química , Acetatos/química , Acetilcolinesterase/efeitos dos fármacos , Animais , Artemia/efeitos dos fármacos , Butirilcolinesterase/efeitos dos fármacos , Inibidores da Colinesterase/farmacologia , Cromatografia Líquida de Alta Pressão/métodos , Células HCT116 , Humanos , Masculino , Metanol/química , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/farmacologia , Ratos Sprague-Dawley , Rubus/classificação , Especificidade da Espécie , Espectrometria de Massas por Ionização por Electrospray/métodos , Cicatrização/efeitos dos fármacos
16.
Mol Nutr Food Res ; 63(8): e1800636, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30763455

RESUMO

SCOPE: Black raspberries (BRB) are a rich source of bioactive phytochemicals, including anthocyanins and ellagitannins. These phytochemicals are poorly absorbed and may be transformed by gut microbiota into various metabolites that may impact the colonic mucosa or upon absorption have systemic bioactivity. The objective of this study is to define the impact of a BRB-containing diet on the colon microbiome in mice and quantify the phytochemical metabolites in the colon contents and circulation. METHODS AND RESULTS: Male mice were fed 10% w/w freeze-dried BRB powder for 6 weeks. The colonic microbiota was evaluated by 16S rRNA gene sequencing. Anthocyanin and ellagitannin metabolites, protocatechuic acid, and urolithins were analyzed by HPLC-MS/MS. The BRB diet impacted colon mucosal microbial composition with a more robust effect observed on the luminal microflora. BRB-derived protocatechuic acid and urolithins were quantified in the colon, luminal contents, plasma, liver, and prostate with protocatechuic acid present in higher concentrations compared to urolithins. CONCLUSION: This study highlights the complex interactions between dietary phytochemicals, the host microbiome, and metabolism. It is demonstrated that microbially produced phytochemical metabolites are present in the colon and systemic circulation where they may exert biological activity.


Assuntos
Colo/microbiologia , Microbioma Gastrointestinal/fisiologia , Rubus , Animais , Peso Corporal , Colo/metabolismo , Cumarínicos/sangue , Cumarínicos/metabolismo , Suplementos Nutricionais , Liofilização , Microbioma Gastrointestinal/genética , Hidroxibenzoatos/sangue , Hidroxibenzoatos/metabolismo , Fígado/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Próstata/metabolismo , RNA Ribossômico 16S , Rubus/química
17.
Chin J Nat Med ; 17(1): 15-21, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30704618

RESUMO

Protein tyrosine phosphatase 1B (PTP1B) has led to an intense interest in developing its inhibitors as anti-diabetes, anti-obesity and anti-cancer agents. The fruits of Rubus chingii (Chinese raspberry) were used as a kind of dietary traditional Chinese medicine. The methanolic extract of R. chingii fruits exhibited significant PTP1B inhibitory activity. Further bioactivity-guided fractionation resulted in the isolation of three PTP1B inhibitory ursane-type triterpenes: ursolic acid (1), 2-oxopomolic acid (2), and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid (3). Kinetics analyses revealed that 1 was a non-competitive PTP1B inhibitor, and 2 and 3 were mixed type PTP1B inhibitors. Compounds 1-3 and structurally related triterpenes (4-8) were further analyzed the structure-activity relationship, and were evaluated the inhibitory selectivity against four homologous protein tyrosine phosphatases (TCPTP, VHR, SHP-1 and SHP-2). Molecular docking simulations were also carried out, and the result indicated that 1, 3-acetoxy-urs-12-ene-28-oic acid (5), and pomolic acid-3ß-acetate (6) bound at the allosteric site including α3, α6, and α7 helix of PTP1B.


Assuntos
Inibidores Enzimáticos/metabolismo , Proteína Tirosina Fosfatase não Receptora Tipo 1/antagonistas & inibidores , Rubus/química , Triterpenos/metabolismo , Inibidores Enzimáticos/química , Frutas/química , Humanos , Cinética , Metanol/química , Simulação de Acoplamento Molecular , Estrutura Molecular , Extratos Vegetais/química , Ligação Proteica , Proteína Tirosina Fosfatase não Receptora Tipo 1/metabolismo , Proteínas Tirosina Fosfatases/antagonistas & inibidores , Relação Estrutura-Atividade , Triterpenos/química
18.
Biomed Res Int ; 2019: 1381989, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30792989

RESUMO

Plant diversity is a basic source of food and medicines for the local communities of the Himalayas. Current study was intended to assess polyphenolics content and antioxidant potential in edible wild fruits used as food and to treat various diseases by the inhabitants of Himalayan region of Pakistan. The fruits of 20 plant species were evaluated using standard protocols, whereas information on medicinal uses was gathered through semistructured interviews. Comparatively, Prunus domestica and Rubus ellipticus fruits exhibited highest levels of phenolics and flavonols contents (113.55 ± 0.61 mg GAE/100 g and 200.06 ± 1.57 mg RtE/100 g FW, respectively) in acetone extract. Nevertheless, flavonoids were maximum in the water extract of Rosa moschata (194.82 ± 3.46 mg RtE/100 g FW). Contrary, Duchesnea indica fruit depicted significant potential to scavenge DPPH and H2O2 radicals at 94.66 ± 8.89% in acetone extract and 83.54± 9.37% in water extract, while acetone extract of Rubus ellipticus had maximum potential to reduce ferric ions (133.66 ± 15.00 µM GAE/100 g FW). Additionally, total antioxidant capacity was highest in the acetone extract of Berberis lycium fruit (332.08 ± 21.90 µM AAE/100 g FW). The relationships between polyphenolics and antioxidant activity revealed synergistic role of secondary metabolites in the prevention of diseases. Our study revealed that wild fruits consumed by the local communities of Himalayas are rich in health beneficial phytochemicals and hold significant potential to treat chronic diseases, particularly associated with free radicals.


Assuntos
Antioxidantes/química , Flavonoides/química , Plantas Comestíveis/química , Polifenóis/química , Frutas/química , Humanos , Peróxido de Hidrogênio/química , Compostos Fitoquímicos/química , Compostos Fitoquímicos/uso terapêutico , Extratos Vegetais/química , Prunus domestica/química , Rubus/química
19.
Oxid Med Cell Longev ; 2019: 9529676, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30723535

RESUMO

Extensive exposure to UVB (280-320 nm) is the major risk responsible for various skin injuries. Numerous reports have shown that natural products could demonstrate photochemopreventive efficacy against UVB damage. We investigated the preventive effects and associated molecular mechanisms of red raspberry extract upon UVB-caused damage in human epidermal keratinocytes and a nude mouse model. The protein profiles and immunohistological study on a nude mouse skin indicated that red raspberry extract could prevent UVB-caused cell death and protect the skin against UVB-exposed injury manifested by wrinkling, scaling, tanning, and water loss as well as epidermal thickening. In addition, red raspberry extract application effectively abolished oxidative damage in DNA and attenuated the carbonylation level of proteins, which attributed to the activation of SOD, Nrf2 and its target genes, and HO-1. Red raspberry extract also altered the cells' apoptotic signaling pathways including caspase-3 as well as the inflammatory cascade such as c-jun and attenuated UVB-induced activation of NF-κB and COX-2. Red raspberry extract could alleviate direct photodamage to the skin caused by UVB exposure through the ROS scavenger and protection against inflammatory responses, which may allow the development of novel strategies in protecting the skin subjected to UVB radiation.


Assuntos
Anti-Inflamatórios/uso terapêutico , Antioxidantes/uso terapêutico , Extratos Vegetais/química , Rubus/química , Pele/fisiopatologia , Raios Ultravioleta/efeitos adversos , Animais , Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Feminino , Humanos , Camundongos , Camundongos Nus
20.
Food Chem ; 275: 417-425, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30724216

RESUMO

The influence of the incorporation of blackberry pulp on properties of arrowroot starch films has been studied. The blackberry pulp transferred bioactive compounds, antioxidant capacity and color to arrowroot starch films. Increasing the concentration of blackberry pulp (from 0 to 40%, mass/mass of dry starch) in the film resulted in increased thickness (from 0.065 to 0.133 mm), increased elongation (from 3.18 to 13.59%), decreased tensile strength (from 22.71 to 3.97 MPa), increased water vapor permeability (from 3.62 to 4.60 g.mm/m2.day.kPa) and solubility in water (from 14.18 to 25.46%). The films were stable to different media, maintaining the same initial diameter dimensions after immersion in acidic, neutral and alkaline solutions, but their color intensity was reduced, probably due to the release of anthocyanins to the solutions. The films with blackberry pulp presented darkness after sterilization process.


Assuntos
Marantaceae/química , Rubus/química , Amido/química , Antocianinas/análise , Antioxidantes/análise , Antioxidantes/farmacologia , Cor , Embalagem de Alimentos/métodos , Concentração de Íons de Hidrogênio , Pasteurização/métodos , Permeabilidade , Solubilidade , Vapor , Resistência à Tração
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