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1.
JAMA ; 323(1): 35-48, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-32207767

RESUMO

Importance: Dietary supplements marketed for male fertility commonly contain folic acid and zinc based on limited prior evidence for improving semen quality. However, no large-scale trial has examined the efficacy of this therapy for improving semen quality or live birth. Objective: To determine the effect of daily folic acid and zinc supplementation on semen quality and live birth. Design, Setting, and Participants: The Folic Acid and Zinc Supplementation Trial was a multicenter randomized clinical trial. Couples (n = 2370; men aged ≥18 years and women aged 18-45 years) planning infertility treatment were enrolled at 4 US reproductive endocrinology and infertility care study centers between June 2013 and December 2017. The last 6-month study visit for semen collection occurred during August 2018, with chart abstraction of live birth and pregnancy information completed during April 2019. Interventions: Men were block randomized by study center and planned infertility treatment (in vitro fertilization, other treatment at a study site, and other treatment at an outside clinic) to receive either 5 mg of folic acid and 30 mg of elemental zinc (n = 1185) or placebo (n = 1185) daily for 6 months. Main Outcomes and Measures: The co-primary outcomes were live birth (resulting from pregnancies occurring within 9 months of randomization) and semen quality parameters (sperm concentration, motility, morphology, volume, DNA fragmentation, and total motile sperm count) at 6 months after randomization. Results: Among 2370 men who were randomized (mean age, 33 years), 1773 (75%) attended the final 6-month study visit. Live birth outcomes were available for all couples, and 1629 men (69%) had semen available for analysis at 6 months after randomization. Live birth was not significantly different between treatment groups (404 [34%] in the folic acid and zinc group and 416 [35%] in the placebo group; risk difference, -0.9% [95% CI, -4.7% to 2.8%]). Most of the semen quality parameters (sperm concentration, motility, morphology, volume, and total motile sperm count) were not significantly different between treatment groups at 6 months after randomization. A statistically significant increase in DNA fragmentation was observed with folic acid and zinc supplementation (mean of 29.7% for percentage of DNA fragmentation in the folic acid and zinc group and 27.2% in the placebo group; mean difference, 2.4% [95% CI, 0.5% to 4.4%]). Gastrointestinal symptoms were more common with folic acid and zinc supplementation compared with placebo (abdominal discomfort or pain: 66 [6%] vs 40 [3%], respectively; nausea: 50 [4%] vs 24 [2%]; and vomiting: 32 [3%] vs 17 [1%]). Conclusions and Relevance: Among a general population of couples seeking infertility treatment, the use of folic acid and zinc supplementation by male partners, compared with placebo, did not significantly improve semen quality or couples' live birth rates. These findings do not support the use of folic acid and zinc supplementation by male partners in the treatment of infertility. Trial Registration: ClinicalTrials.gov Identifier: NCT01857310.


Assuntos
Ácido Fólico/administração & dosagem , Nascimento Vivo , Sêmen/efeitos dos fármacos , Oligoelementos/administração & dosagem , Complexo Vitamínico B/administração & dosagem , Zinco/administração & dosagem , Adulto , Fragmentação do DNA , Suplementos Nutricionais , Feminino , Fertilização In Vitro , Ácido Fólico/efeitos adversos , Humanos , Infertilidade/terapia , Infertilidade Masculina/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Gravidez , Análise do Sêmen , Fatores Socioeconômicos , Contagem de Espermatozoides , Motilidade Espermática , Oligoelementos/efeitos adversos , Resultado do Tratamento , Complexo Vitamínico B/efeitos adversos , Adulto Jovem , Zinco/efeitos adversos
2.
JAMA ; 323(1): 35-48, 2020 01 07.
Artigo em Inglês | MEDLINE | ID: mdl-31910279

RESUMO

Importance: Dietary supplements marketed for male fertility commonly contain folic acid and zinc based on limited prior evidence for improving semen quality. However, no large-scale trial has examined the efficacy of this therapy for improving semen quality or live birth. Objective: To determine the effect of daily folic acid and zinc supplementation on semen quality and live birth. Design, Setting, and Participants: The Folic Acid and Zinc Supplementation Trial was a multicenter randomized clinical trial. Couples (n = 2370; men aged ≥18 years and women aged 18-45 years) planning infertility treatment were enrolled at 4 US reproductive endocrinology and infertility care study centers between June 2013 and December 2017. The last 6-month study visit for semen collection occurred during August 2018, with chart abstraction of live birth and pregnancy information completed during April 2019. Interventions: Men were block randomized by study center and planned infertility treatment (in vitro fertilization, other treatment at a study site, and other treatment at an outside clinic) to receive either 5 mg of folic acid and 30 mg of elemental zinc (n = 1185) or placebo (n = 1185) daily for 6 months. Main Outcomes and Measures: The co-primary outcomes were live birth (resulting from pregnancies occurring within 9 months of randomization) and semen quality parameters (sperm concentration, motility, morphology, volume, DNA fragmentation, and total motile sperm count) at 6 months after randomization. Results: Among 2370 men who were randomized (mean age, 33 years), 1773 (75%) attended the final 6-month study visit. Live birth outcomes were available for all couples, and 1629 men (69%) had semen available for analysis at 6 months after randomization. Live birth was not significantly different between treatment groups (404 [34%] in the folic acid and zinc group and 416 [35%] in the placebo group; risk difference, -0.9% [95% CI, -4.7% to 2.8%]). Most of the semen quality parameters (sperm concentration, motility, morphology, volume, and total motile sperm count) were not significantly different between treatment groups at 6 months after randomization. A statistically significant increase in DNA fragmentation was observed with folic acid and zinc supplementation (mean of 29.7% for percentage of DNA fragmentation in the folic acid and zinc group and 27.2% in the placebo group; mean difference, 2.4% [95% CI, 0.5% to 4.4%]). Gastrointestinal symptoms were more common with folic acid and zinc supplementation compared with placebo (abdominal discomfort or pain: 66 [6%] vs 40 [3%], respectively; nausea: 50 [4%] vs 24 [2%]; and vomiting: 32 [3%] vs 17 [1%]). Conclusions and Relevance: Among a general population of couples seeking infertility treatment, the use of folic acid and zinc supplementation by male partners, compared with placebo, did not significantly improve semen quality or couples' live birth rates. These findings do not support the use of folic acid and zinc supplementation by male partners in the treatment of infertility. Trial Registration: ClinicalTrials.gov Identifier: NCT01857310.


Assuntos
Suplementos Nutricionais , Ácido Fólico/farmacologia , Infertilidade Masculina/tratamento farmacológico , Sêmen/efeitos dos fármacos , Zinco/farmacologia , Adolescente , Adulto , Fragmentação do DNA/efeitos dos fármacos , Suplementos Nutricionais/efeitos adversos , Feminino , Fertilização In Vitro , Ácido Fólico/efeitos adversos , Ácido Fólico/uso terapêutico , Humanos , Nascimento Vivo , Masculino , Pessoa de Meia-Idade , Análise do Sêmen , Contagem de Espermatozoides , Falha de Tratamento , Adulto Jovem , Zinco/efeitos adversos , Zinco/uso terapêutico
3.
Chemosphere ; 245: 125580, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31855762

RESUMO

BACKGROUND: Animal experiments have revealed that pyrethroids (PYRs) exposure could affect semen quality, however evidence on humans being is limited and controversial. OBJECTS: To explore the potential effects of environmental PYRs exposure on semen quality in reproductive age men. METHODS: We conducted a cross-sectional study of 346 men who planned to conceive and addressed to hospital for preconception examination. PYRs exposure was assessed by analyzing PYRs urinary metabolites [3-phenoxybenzoic acid (3PBA), trans- and cis-3-(2,2-Dichlorovinyl) -2,2-dimethylcy clopropane carboxylic acid (TDCCA and CDCCA)] levels using gas chromatography-mass spectrometry. Semen quality was assessed by a computer-aided semen analyzer. RESULTS: For a detection rate of 99.7%, 76.6%, and 22.0%, the median levels (µg/g creatinine) of PYRs metabolites were 0.46 for 3PBA, 0.38 for TDCCA and under detection limit for CDCCA. Linear regression models found negative associations between 3PBA and sperm morphology (ß = -2.12, 95% CI: -4.02 to -0.22) as well as between TDCCA and log-transformed total sperm count (ß = -0.09, 95% CI: -0.16 to -0.01). In logistic regression models, men with the highest quartile of 3PBA had higher risk of poor semen quality (having below-reference semen parameter, OR = 2.40, 95% CI: 1.26 to 4.54; having below-reference sperms morphology, OR = 3.08, 95% CI: 1.10 to 8.60) compared to men in the lowest quartile. CONCLUSIONS: Our study suggests that environmental PYRs exposure might adversely affect semen parameters of reproductive age men in Shanghai, China. Further studies are needed to confirm our findings and demonstrate a causal relationship between PYRs exposure and semen quality.


Assuntos
Praguicidas/toxicidade , Piretrinas/toxicidade , Sêmen/efeitos dos fármacos , Adulto , Benzoatos , China , Estudos Transversais , Exposição Ambiental/estatística & dados numéricos , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Masculino , Piretrinas/metabolismo , Reprodução/efeitos dos fármacos , Análise do Sêmen , Contagem de Espermatozoides , Espermatozoides/efeitos dos fármacos
4.
Res Vet Sci ; 127: 76-81, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31678456

RESUMO

Antimicrobial resistance is increasing within the porcine industry with consequential high impact on human health, leading to a need for new antimicrobials. Lately, the scientific community has turned its interest towards natural compounds, and different essential oils have been tested on spermatozoa for preliminary assessment of toxicity before considering them as good substitutes for standard antibiotics. The aim of the present work was to investigate the potential antimicrobial effect of Melaleuca alternifolia and Rosmarinus officinalis essential oils, already evaluated for toxicity, on swine artificial insemination doses deprived of spermatozoa and stored at 16 °C for 5 days. This was accomplished by setting up an in vitro model with a standardized quantity of E. coli. Essential oils, previously chemo-characterized by means of gas chromatography, were tested at 0.2 and 0.4 mg/ml. Analyses, performed at 24 and 120 h, included optical density evaluation, bacterial DNA quantification by qPCR, and colony count. The results demonstrate that both Melaleuca alternifolia and Rosmarinus officinalis essential oils, at a concentration of 0.4 mg/ml, are capable of delivering similar effects to ampicillin, used as control, on the experimental samples. At the lower concentration, M. alternifolia essential oil seemed more effective when compared to R. officinalis. Overall, these findings strengthen the hypothesis of the potential use of phyto-complexes as antimicrobial agents for reproductive biotechnologies.


Assuntos
Anti-Infecciosos/farmacologia , Escherichia coli/efeitos dos fármacos , Óleos Voláteis/farmacologia , Sêmen/efeitos dos fármacos , Óleo de Melaleuca/farmacologia , Animais , Relação Dose-Resposta a Droga , Testes de Sensibilidade Microbiana , Sêmen/microbiologia , Sus scrofa
5.
Pol J Vet Sci ; 22(3): 463-473, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31560464

RESUMO

Electrophoretic methods were used to identify protein complexes formed between ostrich egg yolk lipoprotein fractions (LPFo) with seminal plasma (SP) of fractionated ejaculates, and to investigate the effect of these complexes on boar semen quality after cryopreservation. Chromatographic SP fractions (F1, F2 and F3), with or without LPFo solution, were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Comparative electrophoretic analyses of the SP revealed marked differences in the SDS-PAGE protein profiles among boars. Electrophoretic analyses showed that the interactions of LPFo with SP resulted in the appearance of high-intensity protein bands. Spermatozoa were exposed to SP chromatographic fractions originating from F1, F2 and F3, and the whole SP (wSP) before being frozen. Spermatozoa exposed to F1 and F2 exhibited significantly higher post-thaw motility compared to those treated with either F3 or wSP. In most of the boars the proportions of membrane- -intact frozen-thawed spermatozoa differed among the treatments, being significantly lower in the wSP-treated samples. The incidence of frozen-thawed spermatozoa with DNA fragmentation was less prevalent in samples exposed to F3 or the wSP. The results of this study confirmed that the interactions of LPFo with fractionated SP during the cooling period contributed to alterations in the sperm membranes, rendering them less susceptible to temperature-related injury.


Assuntos
Gema de Ovo/química , Lipoproteínas/química , Preservação do Sêmen/veterinária , Sêmen/fisiologia , Struthioniformes , Suínos , Animais , Lipoproteínas/farmacologia , Masculino , Sêmen/efeitos dos fármacos , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos
6.
Anim Sci J ; 90(9): 1161-1169, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31381235

RESUMO

Sulfanilamide (SA) is an effective broad-spectrum antibacterial agent in human and veterinary medicine. The purpose of this study was to evaluate the effects of SA on boar sperm quality during liquid storage at 17°C and determine the optimal concentration of SA and its effects on bacterial growth, microbial composition, and maternal fertility. Boar ejaculates were diluted with a basic extender, containing different concentrations of SA, and stored in a 17°C incubator for 6 days. The sperm motility, plasma membrane integrity, and acrosome integrity were measured daily. The results showed that when the concentration of SA was 0.02 g/L, the sperm quality parameters were significantly higher than those of all other treatment groups (p < .05). We also monitored the bacterial growth and compared the differences in the microbial species between the 0.02 g/L SA group and the control by 16S rDNA sequencing. The results revealed that some bacteria, such as Staphylococcus and Pseudomonas, were considerably lower in the 0.02 g/L SA group than in the control group (p < .05). In addition, preserved semen was used for artificial insemination, and results showed that 0.02 g/L SA group had a higher litter size, and its pregnancy rate was 92.5%.


Assuntos
Análise do Sêmen/veterinária , Preservação do Sêmen/veterinária , Sulfanilamida/farmacologia , Acrossomo/efeitos dos fármacos , Animais , Feminino , Fertilidade/efeitos dos fármacos , Inseminação Artificial/veterinária , Tamanho da Ninhada de Vivíparos/efeitos dos fármacos , Masculino , Sêmen/efeitos dos fármacos , Preservação do Sêmen/métodos , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Espermatozoides/microbiologia , Suínos
7.
Anim Reprod Sci ; 208: 106127, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31405456

RESUMO

The purpose of the current study was to evaluate the effects of resveratrol (RSV) on the quality of frozen-thawed goat sperm. Semen samples from four bucks were divided into five aliquots and diluted with a commercial bull semen extender containing: no antioxidant (RSV-0, control), 10 µM RSV (RSV-10), 50 µM RSV (RSV-50), 100 µM RSV (RSV-100) and 250 µM RSV (RSV-250). After thawing, sperm motility, abnormal morphology, membrane and acrosome integrity, mitochondrial activity, phosphatidylserine (PS) distribution, and oxidative stress were evaluated. The results indicated that in comparison with the control, when the concentration of RSV was 10 or 50 µM, the total motility, progressive motility, membrane and acrosome integrity, and mitochondrial activity of post-thaw spermatozoa was greater (P < 0.05). Additionally, the use of extenders containing RSV-10 or RSV-50 resulted in a greater percentage of viable spermatozoa as compared to the other groups (P < 0.05). Importantly, there were more viable spermatozoa (49.61 ±â€¯0.61%) and less non-viable spermatozoa (49.16 ±â€¯1.01%) in the RSV-50 group compared to the other extenders (P < 0.05). Furthermore, the use of the extenders containing RSV-10 and -50 resulted in a reduction in ROS production in frozen-thawed spermatozoa as compared to the control (P < 0.05). There, however, was no difference among extenders for abnormal morphology and PS distribution. In conclusion, supplementation with RSV, at a concentration of 10 or 50 µM in the semen extender, can improve the post-thaw goat sperm quality, which may occur as a consequence of inhibition of ROS generation.


Assuntos
Criopreservação/veterinária , Cabras , Resveratrol/farmacologia , Análise do Sêmen/veterinária , Preservação do Sêmen/veterinária , Sêmen/efeitos dos fármacos , Animais , Antioxidantes/farmacologia , Membrana Celular/efeitos dos fármacos , Crioprotetores/farmacologia , Masculino , Estresse Oxidativo/efeitos dos fármacos , Sêmen/fisiologia , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos
8.
Anim Reprod Sci ; 208: 106130, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31405463

RESUMO

The objective of this study was to evaluate the effect of adding reduced glutathione (GSH) to a boar semen freezing extender supplemented with insulin-like growth factor I (IGF-I) or anti-IGF-I. Eight ejaculates from eight boars were extended to obtain insemination doses, which were supplemented with either recombinant human IGF-I (30 ng/mL) or anti-IGF-I (60 ng/mL) shortly after extension. After 24 h of liquid storage at 17 °C, the semen was frozen with or without GSH (5 mM) in the freezing extender for a total of six treatments. Osmotic resistance and acrosome integrity was greater in fresh semen (P <  0.05) soon after adding IGF-I or the anti-IGF-I antibody. After 24 h of cooling, the supplementation with these compounds resulted in an increased (P <  0.05) percentage of sperm with relatively greater mitochondrial activity and reduced the percentage of cells with relatively greater concentrations of superoxide. After thawing, there was a reduction (P <  0.05) in the percentage and fluorescence intensity of sperm with greater quantities of superoxide and peroxide only in samples treated with GSH + IGF-I and GSH + anti-IGF-I. The addition of GSH (alone or in combination with IGF-I or anti-IGF-I), however, reduced the percentage of sperm with an intact acrosome (P < 0.05). The same effect was not observed with IGF-I or anti-IGF-I alone. In conclusion, the addition of IGF-I or anti-IGF-I improved the quality of fresh or liquid-stored semen. Using GSH in the freezing extender improved the antioxidant potential of frozen semen only in combination with IGF-I or an anti-IGF-I antibody.


Assuntos
Criopreservação/veterinária , Glutationa/farmacologia , Fator de Crescimento Insulin-Like I/farmacologia , Preservação do Sêmen/veterinária , Sêmen/efeitos dos fármacos , Suínos , Animais , Antioxidantes/farmacologia , Benzoxazóis/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Fluoresceínas/farmacologia , Corantes Fluorescentes , Masculino , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Compostos de Quinolínio/farmacologia , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Espermatozoides/fisiologia
9.
Anim Reprod Sci ; 208: 106103, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31405464

RESUMO

The purpose of this study was to evaluate the effect of different concentrations of CoQ10 in soybean lecithin (SL) or egg yolk (EY) extenders on ram semen cryopreservation. Semen samples were collected from five rams, twice a week, then diluted in the extenders (SL and EY) containing different concentrations of CoQ10 as follows: extender containing SL: 0 µM (control, SL/Q0), 1 µM (SL/Q1), 2 µM (SL/Q2), 5 µM (SL/Q5) and 10 µM (SL/Q10) CoQ10; extender containing EY: 0 µM (control, EY/Q0), 1 µM (EY/Q1), 2 µM (EY/Q2), 5 µM (EY/Q5) and 10 µM (EY/Q10) CoQ10. Sperm motion characteristics, membrane integrity, abnormal morphology, viability, apoptotic-like changes, mitochondria active potential, acrosome integrity and lipid peroxidation were evaluated after freeze-thaw process. The SL/Q1, SL/Q2, EY/Q1 and EY/Q2 resulted in greater (P ≤ 0.05) sperm total motility, progressive motility, membrane integrity and mitochondria active potential compared to the other groups. Acrosome integrity in the SL/Q0, SL/Q1, SL/Q2, EY/Q0, EY/Q1 and EY/Q2 groups was greater (P ≤ 0.05) than in the SL/Q5, SL/Q10, EY/Q5 and EY/Q10 groups. The SL/Q2 and EY/Q2 treatment groups had greater (P ≤ 0.05) sperm viability rates and less apoptotic-like changes and lipid peroxidation. The CoQ10 compound could be explored as a novel potential antioxidant for cryopreservation of ram semen because with used of this compound in the present study there was an improved post-thawed sperm quality.


Assuntos
Gema de Ovo , Lecitinas/farmacologia , Preservação do Sêmen/veterinária , Ovinos , Espermatozoides/efeitos dos fármacos , Ubiquinona/análogos & derivados , Animais , Antioxidantes/farmacologia , Criopreservação/métodos , Criopreservação/veterinária , Congelamento , Peroxidação de Lipídeos , Masculino , Sêmen/efeitos dos fármacos , Preservação do Sêmen/métodos , Ubiquinona/farmacologia
10.
Anim Reprod Sci ; 208: 106128, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31405471

RESUMO

The aim was to evaluate effects of addition of pentoxifylline to skimmed milk semen extender on uterine inflammatory response. Thirty-six estrous cycles of 15 mares were randomly divided into five groups for artificial insemination (AI): Control: mimicking the AI procedure (n = 7); Extender: deposition of skimmed milk based extender (n = 7); Extender + PTX: skimmed milk based extender plus pentoxifylline (7.18 mM; n = 8); Semen: semen diluted with extender without pentoxifylline (n = 7), and Semen + PTX: semen diluted with extender containing pentoxifylline (n = 7). Mares in estrus were examined by trans-rectal palpation and using ultrasonography, and ovulation was induced. Uterine hemodynamics were assessed immediately before ovulation induction (T-30), immediately before AI (T0), 2 (T2), 6 (T6), 12 (T12), 24 (T24) and 48 (T48) h after AI. Endometrial samples were collected 6 h after AI, and slides were stained and examined to determine percentage of PMN. Pentoxifylline had no additional effect on vascular perfusion. There was a major inflammatory response with pentoxifylline treatment that was greater than that of the control group. In the group treated with Extender + PTX, there were more PMN (57.98 ±â€¯9.42%) than in the group treated with Extender (20.20 ±â€¯6.63%) and in the Semen + PTX group more PMN (82.84 ±â€¯5.71%) than in the Semen-treated group (47.83 ±â€¯10.61%). These findings indicate the addition of pentoxifylline does not stimulate blood flow; however, it induces a greater immune defense response because more neutrophils migrate to the uterine lumen.


Assuntos
Doenças dos Cavalos/prevenção & controle , Inflamação/veterinária , Pentoxifilina/farmacologia , Sêmen/efeitos dos fármacos , Doenças Uterinas/veterinária , Animais , Estudos Cross-Over , Endométrio/irrigação sanguínea , Endométrio/efeitos dos fármacos , Feminino , Cavalos , Inflamação/prevenção & controle , Inseminação Artificial/veterinária , Masculino , Leite , Ultrassonografia/veterinária , Doenças Uterinas/prevenção & controle , Vasodilatadores/farmacologia
11.
Niger J Physiol Sci ; 34(1): 83-90, 2019 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-31449276

RESUMO

The present study examines the possible ameliorative effects of the hydromethanolic extract of Citrullus lanatus rind (HECL) on some reproductive function and oxidative indices of the testes in male Wistar rats following administration of nicotine. Twenty male rats were assigned into four groups: Group A to D of five rats each. Group A served as control and received 2ml/kg body weight of 10% extract vehicle; Group B received 1mg/kg body weight of nicotine; Group C were co-administered 1mg/kg body weight nicotine and 500 mg/kg body weight of HECL and Group D received only 500mg/kg body weight of HECL. The drugs and extracts were administered orally to the rats for 42days; blood samples were collected by direct cardiac puncture for determination of serum concentrations of testosterone, Follicle Stimulating Hormone and Luteinizing Hormone. The testes were also harvested for determination of semen parameters: motility, morphology, viability and count and testicular tissue processed for superoxide dismutase and malondialdehyde concentration. Compared to Group A control rats, administration of HECL significantly increased sperm count and reproductive hormone concentrations amongst Group B rats (p<0.05). Treatment with nicotine caused a significant reduction in the levels of all reproductive hormones with significant diminution of some sperm parameters: motility, morphology and viability; and decrease in superoxide dismutase and increase in malondialdehyde concentration amongst Group B rats compared to Group A control rats (p<0.05). Co-administration of HECL with nicotine to Group C rats apparently reversed the effects of nicotine resulting in significant increases in sperm count and the reproductive hormones concentration as compared to Group A control rats (p<0.05). Amongst Group D rats, the extract also caused a significant increase in superoxide dismutase concentration and a significant decrease in malondialdehyde concentration compared with the Group A control rats (p<0.05). The findings suggest that the hydromethanolic extract of Citrullus lanatus rind possibly ameliorates the deleterious effects of nicotine on some reproductive indices in male Wistar rats.


Assuntos
Citrullus , Hormônios Esteroides Gonadais/sangue , Nicotina/toxicidade , Extratos Vegetais/farmacologia , Sêmen/efeitos dos fármacos , Testículo/efeitos dos fármacos , Animais , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Hormônios Esteroides Gonadais/antagonistas & inibidores , Masculino , Metanol/farmacologia , Nicotina/administração & dosagem , Agonistas Nicotínicos/administração & dosagem , Agonistas Nicotínicos/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/fisiologia , Ratos , Ratos Wistar , Sêmen/metabolismo , Testículo/metabolismo , Resultado do Tratamento
12.
Trials ; 20(1): 540, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31464617

RESUMO

BACKGROUND: In Hong Kong, one of six couples is affected by subfertility problems. Male infertility contributes to half of the infertility cases. In male infertility, there is no effective treatment for patients with idiopathic infertility/poor semen parameters. Recent meta-analysis results suggest that a traditional Chinese medicine (TCM) formula - Wuzi Yanzong pill - showed a curative effect on male fertility. However, the heterogeneity of the studies could not draw a definitive conclusion on the therapeutic effect of this formula. The aim of this study is to conduct a well-designed randomized controlled trial to investigate the effect of TCM formula Wuzi Yanzong pill on improving semen qualities in men with suboptimal parameters. METHODS: This study is a double-blinded, randomized placebo-controlled trial conducted in a public hospital in Hong Kong. Participants will be randomized, using computer-generated random numbers, with a 1:1 ratio to either the Wuzi Yanzong pill formula group or the placebo group. Both groups will be administered the drugs for 12 weeks. Participants will have a total of four visits for their semen and blood assessments for a 6-month period, and we will follow up for another 6 months to record their conception outcome. The primary outcome is to compare the total motile sperm count, natural conception rate, and pregnancy outcome to those under placebo treatment. Secondary objectives are sperm functions and assisted reproductive technology outcome. DISCUSSION: To date, there are no studies using the disclosed Wuzi Yanzong formula or double-blinded, randomized trials. The Wuzi Yanzong TCM formula may provide a good clinical solution for subfertile males for which contemporary western medicine has no cure. Therefore, a well-designed randomized trial for evaluating the effect of Wuzi Yanzong TCM formula is urgently needed. TRIAL REGISTRATION: Chinese Clinical Trial Registry (ChiCTR), ChiCTR-INR-17010790 . Registered on 27 February 2017. Centre for Clinical Research and Biostatistics - Clinical Trials Registry, CUHK_CCRB00548 . Registered on 27 February 2017.


Assuntos
Medicamentos de Ervas Chinesas/administração & dosagem , Fármacos para a Fertilidade Masculina/administração & dosagem , Fertilidade/efeitos dos fármacos , Infertilidade Masculina/tratamento farmacológico , Sêmen/efeitos dos fármacos , Administração Oral , Método Duplo-Cego , Medicamentos de Ervas Chinesas/efeitos adversos , Estudos de Equivalência como Asunto , Fármacos para a Fertilidade Masculina/efeitos adversos , Hong Kong , Humanos , Infertilidade Masculina/diagnóstico , Infertilidade Masculina/fisiopatologia , Masculino , Estudos Prospectivos , Análise do Sêmen , Comprimidos , Fatores de Tempo , Resultado do Tratamento
13.
J Trace Elem Med Biol ; 55: 6-14, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31345367

RESUMO

INTRODUCTION: Dietary boron improves immune and antioxidant status and calcium metabolism in mammals. However, till date the effects of dietary boron supplementation on male reproduction, especially on sperm production and sperm quality in farm animals are not documented. OBJECTIVE: The present study was aimed to investigate the influence of dietary boron on semen production, semen quality, immunity and molecular changes in the testis, blood and seminal plasma and to assess the interrelationship with other minerals in male goats. METHODOLOGY: The study was conducted in 21 adult male goats divided into 3 groups (control, boron and selenium supplemented groups, n = 7 each). In boron group, boron was supplemented at 40 ppm and in selenium group, selenium was supplemented at 1 ppm over and above the basal level. In control group, only the basal diet was fed without supplementary boron or selenium. The feeding trial was carried out for 60 days. Selenium was taken as a positive control for the dietary boron supplementation experiment. Following feeding trials, the sperm concentration, kinematics and functional attributes, immunity and molecular level changes in the testis, biomolecular changes in the blood and seminal plasma and also interrelationship with other minerals were studied. RESULTS: The average sperm concentration (million/ml) and the total sperm production (million/ejaculate) were significantly (p < 0.05) increased in boron supplemented group when compared to selenium and control groups. The boron levels in blood plasma (r = 0.65) and seminal plasma (r = 0.54) showed a positive correlation with sperm progressive motility. Blood and seminal plasma metabolic biomarker namely, aspartate aminotransferase (AST) (p < 0.01) was significantly lower in the boron and selenium supplemented group than control, while alanine aminotransferase (ALT) (p < 0.05) was significantly lower in the boron supplemented group than selenium and control group. There was a significant increase in the mRNA expression of serine proteinase inhibitor (SERPIN) and interferon γ (IFNγ) in the testis of boron supplemented than the control group. Boron supplementation up-regulated the immune-regulatory gene, interleukin 2 (IL2) and antioxidant gene, catalase (CAT) in the peripheral blood mononuclear cells (PBMC). On contrary, toll-like receptor 2 (TLR2) mRNA expression was significantly (p < 0.05) down-regulated in boron and selenium supplemented groups. CONCLUSION: The study revealed that dietary boron supplementation increased the sperm output, sperm motility and enhanced the immune and antioxidant defense capacity in male goats. The improved semen quality can be attributed to enhanced expression of testicular SERPIN, a crucial protein for the regulation of spermatogenesis process.


Assuntos
Boro/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Sêmen/efeitos dos fármacos , Sêmen/imunologia , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Testículo/efeitos dos fármacos , Animais , Boro/administração & dosagem , Suplementos Nutricionais , Regulação da Expressão Gênica/genética , Cabras , Masculino , Minerais/química , Minerais/isolamento & purificação , RNA Mensageiro/genética , Selênio/administração & dosagem , Selênio/farmacologia , Análise do Sêmen , Inibidores de Serinopeptidase do Tipo Kazal/genética , Motilidade Espermática/imunologia , Espermatozoides/imunologia , Testículo/imunologia
14.
Anim Reprod Sci ; 207: 95-106, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31208848

RESUMO

This study was conducted to assess the effects of commercial extenders and storage temperature on dromedary camel sperm quality during liquid preservation. In Experiment 1, ejaculates (n = five males; replicated seven times) were split and diluted with synthetic (OPTIXcell, EquiPlus, INRA96, Bioxcell or AndroMed; Experiment 1a) or egg-yolk based (Biladyl, Green buffer or Triladyl; Experiment 1b) extenders and stored for 48 h at 4 °C. In Experiment 2, split ejaculates (n = five males; replicated six times) were used to directly compare Green buffer, OPTIXcell and Triladyl extenders over 48 h of storage at 4 °C. Ejaculates collected in Experiment 3 (n = five males; replicated five times) were diluted with Green buffer or Triladyl before chilled storage for 48 h at 4 or 15 °C. Sperm kinematics, viability and acrosome integrity were assessed during liquid storage. In Experiment 1a, there was the greatest total sperm motility (TM) in the OPTIXcell group following 24 and 48 h of storage, while in Experiment 1b, there was the greatest TM after 48 h of storage with Triladyl and Green buffer. In Experiment 2, there were greater TM and viable acrosome intact spermatozoa in the Triladyl and Green buffer than with OPTIXcell group. In Experiment 3, there was a greater TM in the Triladyl than Green buffer group at 24 and 48 h of storage regardless of storage temperature (which had no effect on sperm quality). In conclusion, camel sperm have greater viability when preserved in liquid form for 48 h following dilution with Triladyl and storage at either 4 or 15 °C.


Assuntos
Camelus , Soluções para Preservação de Órgãos/farmacologia , Refrigeração , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Sêmen/efeitos dos fármacos , Animais , Tampões (Química) , Sobrevivência Celular/efeitos dos fármacos , Gema de Ovo/fisiologia , Soluções Isotônicas/farmacologia , Masculino , Soluções para Preservação de Órgãos/química , Refrigeração/métodos , Refrigeração/veterinária , Análise do Sêmen , Espermatozoides/efeitos dos fármacos , Espermatozoides/fisiologia , Temperatura , Fatores de Tempo
15.
Anim Reprod Sci ; 207: 73-82, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31208851

RESUMO

A dopamine type-2 receptor (DRD2) SNP, previously found to be correlated with serum prolactin (PRL) concentrations in cattle, was evaluated for impact on growth traits, serum prolactin concentration, and semen quality. Over a four-year period, yearling beef bulls were allowed diets containing or lacking ergot alkaloids (EA). Every 21 or 28 d semen was collected for semen motility and morphology assessment and blood samples were collected to measure serum PRL concentrations. In addition, body condition score and scrotal circumference were evaluated. Serum PRL concentrations were assessed using a radioimmunoassay. In the first year, all bulls were sacrificed at the end of a 126-day study. Testicles and epididymis were collected at the end of the study or 60 days after removal from treatment. Immunohistochemistry was performed on testis, epididymis, and sperm cells, incubated with or without a primary antibody for DRD2 and counterstained with DAPI. Isolation of DNA was performed on sperm pellets using DNAzol (Thermo Fisher Scientific, Waltham, MA, USA) methods. Polymerase chain reaction was performed to amplify the region of the DRD2 gene containing the SNP of interest. The products were subjected to restriction fragment length polymorphism analysis. Further, all samples were subjected to genotyping using a custom Taqman genotyping assay (Applied Biosystems, Foster city, CA, USA). The presence of DRD2 was detected in the testis, epididymis, and sperm cells. The DRD2 genotype was not associated with semen quality, serum PRL, or growth traits. Consumption of EA resulted in lesser PRL serum concentrations but had no effect on values for other variable examined.


Assuntos
Bovinos , Agonistas de Dopamina/farmacologia , Crescimento e Desenvolvimento , Polimorfismo de Nucleotídeo Único , Prolactina/sangue , Receptores de Dopamina D2/genética , Sêmen/efeitos dos fármacos , Animais , Constituição Corporal/genética , Bovinos/sangue , Bovinos/genética , Bovinos/crescimento & desenvolvimento , Dopamina/sangue , Genótipo , Crescimento e Desenvolvimento/efeitos dos fármacos , Crescimento e Desenvolvimento/genética , Masculino , Sêmen/metabolismo , Sêmen/fisiologia , Análise do Sêmen/veterinária , Motilidade Espermática/efeitos dos fármacos , Motilidade Espermática/genética
16.
Poult Sci ; 98(11): 6100-6107, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31222318

RESUMO

An excessive amount of aromatase enzyme reduces reproductive performance in aging roosters. Testosterone metabolism by aromatase enzyme is one of the reasons for reduced testosterone and lower fertility of aging roosters. The purpose of this study was to determine the effects of Exemestane (EX), as a steroidal aromatase inhibitor, on the seminal parameters and reproductive hormones of aging roosters. A total of 20 roosters (45 wk of age) were housed in individual cages and received a standard basal diet and oral EX capsules for 60 D at the daily doses per rooster (mg/rooster) in the following experimental groups: 0 mg (CTRL), 0.25 mg (Ex-0.25), 0.5 mg (Ex-0.5), and 1.5 mg (Ex-1.5). Sperm samples were obtained on days 1, 20, 40, and 60 of experiment. Blood samples were taken on days 1 and 60. The results indicated that different EX dosages affected semen parameters (P < 0.05) other than semen volume, morphology, apoptosis, and acrosome integrity. Various semen characteristics were significant (P < 0.05) during different times of the experiment, with the exception of semen volume, total motility, membrane integrity, morphology, apoptosis, and acrosome integrity. Roosters that received 0.5 mg of EX had higher percentages of sperm concentration, total motility, progressive motility, membrane integrity, viability, and mitochondrial activity (P < 0.05). There were lower concentrations of malondialdehyde in the CTRL (0 mg) and Ex-0.25 groups (P < 0.05). Although there was no significant difference in hormones at day 0 of the experiment (P > 0.05), roosters in the Ex-0.5 had higher concentration of testosterone as well as lower of aromatase activity at day 60 (P < 0.05). It can be concluded that EX improved semen parameters and testosterone, which ultimately can increase fertility in the aging broiler breeder roosters.


Assuntos
Androstadienos/farmacologia , Inibidores da Aromatase/farmacologia , Aromatase/metabolismo , Galinhas/fisiologia , Sêmen/efeitos dos fármacos , Testículo/efeitos dos fármacos , Animais , Proteínas Aviárias/metabolismo , Hormônio Foliculoestimulante/sangue , Hormônio Luteinizante/sangue , Masculino , Sêmen/fisiologia , Análise do Sêmen/veterinária , Testículo/fisiologia , Testosterona/sangue
17.
BMC Complement Altern Med ; 19(1): 132, 2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31208410

RESUMO

BACKGROUND: Nigerian Cannabis sativa (hemp) causes male gonadotoxicity by inducing hyperprolactinemia, down-regulation of hypothalamic-pituitary-testicular axis, and oxidative stress. Benin republic hemp has been preferred by illicit users in Nigeria but its effect on male fertility is not understood. This study determined and compared the compositions of Benin republic hemp ethanol extract (BHE) and Nigerian hemp. The effects of BHE on semen parameters, reproductive hormones, and anti-oxidant status, and the possibility of bromocriptine (prolactin inhibitor) to abolish hemp-induced toxicities in rats were also investigated. METHODS: Thirty-six male Wistar rats were blindly randomized into 6 oral treatment groups (n = 6 each). Groups I (control) and II received normal saline and bromocriptine (3 mg/kg) respectively. Groups III and IV received 2 mg/kg of BHE alone and in combination with bromocriptine respectively, while groups V and VI received 10 mg/kg BHE alone and in combination with bromocriptine respectively. Comparisons among the groups were done by one-way analysis of variance, followed by post-hoc Tukey multiple comparison test. Statistical significance was considered at p < 0.05. RESULTS: The BHE has no cannabichromene and tetrahydrocannabinol but a very small quantity of cannabinol and higher quantity of fatty acids when compared to Nigerian hemp. Both doses of BHE increased sperm count, morphology and viability but not motility. Co-administration of BHE with bromocriptine lowered sperm count but increased sperm morphology and viability. Bromocriptine and/or BHE caused reduction in the plasma prolactin level, increase in the plasma superoxide dismutase activity, but no significant change in the plasma gonadotropin releasing hormone, follicle stimulating hormone (except for the increase in rats that received bromocriptine+ 10 mg/kg BHE), luteinizing hormone, estradiol, malondialdehyde and glutathione peroxidase. The 10 mg/kg BHE or bromocriptine+BHE (both doses) increased total anti-oxidant capacity and catalase. CONCLUSIONS: The BHE improves semen parameters by reducing plasma prolactin and enhancing plasma anti-oxidant status. Its pro-fertility potential might be associated with its deficiency in the widely known gonadotoxic phytocannabinoids.


Assuntos
Antioxidantes/metabolismo , Canabinoides/análise , Cannabis/química , Dronabinol/análise , Fármacos para a Fertilidade Masculina/farmacologia , Extratos Vegetais/farmacologia , Prolactina/metabolismo , Sêmen/efeitos dos fármacos , Animais , Canabinoides/farmacologia , Dronabinol/farmacologia , Fertilidade/efeitos dos fármacos , Fármacos para a Fertilidade Masculina/análise , Masculino , Extratos Vegetais/análise , Ratos , Sêmen/metabolismo
18.
Anim Reprod Sci ; 207: 107-117, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31204090

RESUMO

Nerve growth factor-ß (NGF) is a seminal plasma protein associated with improved sperm membrane integrity and motility in mammalian species. The objective of this study was to compare post-thaw semen quality from both ejaculated and pididymal-collected bull sperm incubated with purified NGF prior to cryopreservation. Semen was obtained from Angus × Simmental crossbred bulls (n = 10) collected by electroejaculation, followed by castration and epididymal sperm collections 3 days later. Semen samples were incubated with extender having 0 ng/mL (CONT), 0.5 ng/mL (LOW), 5 ng/mL (MED), or 50 ng/mL (HIGH) of purified NGF prior to cryopreservation. Sperm motility was assessed in each sample prior to treatment and cryopreservation and at post-thaw. Flow cytometry was used for post-thaw assessment of sperm viability (SYBR-14/PI), acrosome integrity (FITC-PNA/PI), and chromatin stability (acridine orange). Values for post-thaw sperm motility and velocity variables were decreased, while linearity was increased in samples of the HIGH compared with CONT group (P < 0.01), but there were no differences in epididymal samples (P> 0.05). Samples from the HIGH group also had a lesser amplitude of lateral head displacement at 2.5 and 3 h post-thaw (P < 0.01). Post-thaw sperm viability, acrosome integrity, and DNA fragmentation index were not affected by NGF treatment in either ejaculated or epididymal sperm (P> 0.05). In conclusion, supplementation of freezing extender with NGF had minimal effects on post-thaw sperm quality in bulls. Results indicate NGF may have a function in preventing premature sperm hyperactivation in ejaculated, but not epididymal-collected spermatozoa. Fertility studies, both in vitro and in vivo, are warranted to ascertain the relevancy of these findings.


Assuntos
Bovinos , Criopreservação , Crioprotetores/farmacologia , Fator de Crescimento Neural/farmacologia , Preservação do Sêmen , Sêmen/efeitos dos fármacos , Animais , Criopreservação/métodos , Criopreservação/veterinária , Crioprotetores/administração & dosagem , Combinação de Medicamentos , Ejaculação/fisiologia , Estimulação Elétrica , Congelamento/efeitos adversos , Masculino , Fator de Crescimento Neural/administração & dosagem , Sêmen/fisiologia , Análise do Sêmen/veterinária , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Recuperação Espermática
19.
Int J Occup Med Environ Health ; 32(3): 387-399, 2019 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-31180071

RESUMO

OBJECTIVES: The purpose of this paper was to analyze the relationship between the selected chemical air pollutants found in the Lublin Province and the semen parameters of men seeking fertility treatment for the first time. MATERIAL AND METHODS: The study involved an analysis of semen sample test results obtained from male patients first reporting for fertility treatment in reproductive health centers in the Lublin Province, Poland. The data set comprises semen parameters of 13 148 men, and the number of samples in the reference period was 255-769 annually. Data on air pollution were obtained from the website of the Polish General Environmental Inspectorate and included selected chemical air pollutant levels, i.e., NO2, SO2, O3, and PM10. RESULTS: The mean PM10 levels in the air increased, on average, by 0.65 µg/m3 annually in 2000-2015 (p = 0.029); the mean levels of O3, NO2, SO2 did not change significantly in the analyzed period. There were increasing trends in the mean sperm density and total sperm count of the subjects in 1992-2015 (p < 0.001). The mean percentage of sperm with normal morphology significantly decreased in the subsequent analysis periods: 1992-1998 (p = 0.001); 1999-2009 (p < 0.001); 2010-2015 (p = 0.001). A significant negative correlation was found between the ozone levels in the air in the Lublin Province and the percentage of sperm with normal morphology (r = -0.8311, p = 0.040). CONCLUSIONS: Exposure to ozone in the air contributes to decreased percentages of sperm with normal sperm morphology. Over the years, there was an increasing trend in sperm density in the men first reporting for fertility treatment, and a decreasing trend in the percentage of sperm with normal morphology. Int J Occup Med Environ Health. 2019;32(3):387-99.


Assuntos
Poluição do Ar/efeitos adversos , Material Particulado/efeitos adversos , Análise do Sêmen , Adulto , Poluentes Atmosféricos/efeitos adversos , Humanos , Infertilidade Masculina , Masculino , Dióxido de Nitrogênio/efeitos adversos , Ozônio/efeitos adversos , Polônia , Sêmen/efeitos dos fármacos , Contagem de Espermatozoides , Espermatozoides/anormalidades , Dióxido de Enxofre/efeitos adversos
20.
Poult Sci ; 98(11): 6071-6077, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31180125

RESUMO

The aim was to compare the effect of dimethylacetamide (DMA) and N-methylacetamide (NMA) concentrations on the quality and fertility of post-thaw chicken semen. Ejaculates were obtained from 30 Hi-Line White roosters and processed according to the following treatments: lake pre-freezing extender + 0.1 M trehalose (LPF-T) + 6% DMA (control treatment), LPF-T + 9% DMA, LPF-T + 6% NMA, and LPF-T + 9% NMA. Sperm quality (viability, motility, and kinetic traits) was assessed before and after cryopreservation. A total of 15 laying hens per treatment were inseminated to assess fertility and embryo viability. Sperm cryopreserved in presence of DMA had significantly better in vitro quality compared to NMA, showing the highest proportion of viable and progressive motile sperm recovered after thawing. Furthermore, proportion of progressive motile sperm and the VCL, LIN, ALH, and WOB mean values were significantly improved in semen samples frozen/thawed with 6% compared to 9% cryoprotectant concentration. However, the best cryoprotective action on sperm quality played by DMA and the lowest cryoprotectant concentration did not translate into a concomitant advantage in in vivo semen fertility that showed no differences between cryoprotectant and cryoprotectant concentration treatments. Finally, the cryoprotectant DMA and NMA showed an opposite effect on embryo viability in comparison with the effect played on in vitro semen quality, being NMA more efficient than DMA on preserving viable embryos. The present results suggest the urgency to further decrease the cryoprotectant concentration in poultry semen freezing procedures and to assess the specific toxic effect of cryoprotectant on sperm integrity, fertility, and embryo development.


Assuntos
Acetamidas/farmacologia , Galinhas/fisiologia , Crioprotetores/farmacologia , Fertilidade/efeitos dos fármacos , Análise do Sêmen/veterinária , Sêmen/efeitos dos fármacos , Animais , Congelamento , Masculino
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