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1.
Int J Syst Evol Microbiol ; 70(12): 6307-6312, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33090949

RESUMO

During a study of yeast diversity in Azorean vineyards, four strains were isolated which were found to represent a novel yeast species based on the sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) and of the D1/D2 domain of the large subunit (LSU) rRNA gene, together with their physiological characteristics. An additional strain isolated from Drosophila suzukii in Italy had identical D1/D2 sequences and very similar ITS regions (five nucleotide substitutions) to the Azorean strains. Phylogenetic analysis using sequences of the ITS region and D1/D2 domain showed that the five strains are closely related to Clavispora lusitaniae, although with 56 nucleotide differences in the D2 domain. Intraspecies variation revealed between two and five nucleotide differences, considering the five strains of Clavispora santaluciae. Some phenotypic discrepancies support the separation of the new species from their closely related ones, such as the inability to grow at temperatures above 35 °C, to produce acetic acid and the capacity to assimilate starch. Neither conjugations nor ascospore formation were observed in any of the strains. The name Clavispora santaluciae f.a., sp. nov., is proposed to accommodate the above noted five strains (holotype, CBS 16465T; MycoBank no., MB 835794).


Assuntos
Filogenia , Saccharomycetales/classificação , Vitis/microbiologia , DNA Fúngico/genética , DNA Intergênico/genética , DNA Espaçador Ribossômico/genética , Itália , Técnicas de Tipagem Micológica , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA
2.
J Mycol Med ; 30(4): 101042, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32919860

RESUMO

Candida nivariensis is a cryptic fungal species classified within the Candida glabrata complex. It was described for the first time in 2005 by the means of DNA sequencing. We report a rare case of C. nivariensis deep-seated infection occurring in a 77-year-old man hospitalized for cysto-prostatectomy. Phenotypic testing based on the direct examination and the macroscopic features of the in vitro culture initially suggested C. glabrata species, while MALDI-TOF mass spectrometry enables correct identification. The isolate was found resistant to fluconazole, like in almost 20% of the reported cases. Herein, we present our practical strategy to reliably characterize this rare cryptic species. To date, MALDI-TOF mass spectrometry-based analysis showed very good results for such a purpose.


Assuntos
Candidemia/microbiologia , Saccharomycetales/classificação , Saccharomycetales/isolamento & purificação , Adenocarcinoma de Pulmão/complicações , Adenocarcinoma de Pulmão/imunologia , Adenocarcinoma de Pulmão/microbiologia , Idoso , Candidemia/etiologia , Carcinoma de Células de Transição/microbiologia , Carcinoma de Células de Transição/patologia , França , Humanos , Neoplasias Pulmonares/complicações , Neoplasias Pulmonares/imunologia , Neoplasias Pulmonares/microbiologia , Masculino , Testes de Sensibilidade Microbiana , Técnicas de Tipagem Micológica/métodos , Recidiva , Neoplasias da Bexiga Urinária/microbiologia , Neoplasias da Bexiga Urinária/patologia , Urotélio/patologia
3.
Int J Syst Evol Microbiol ; 70(11): 5665-5670, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32924919

RESUMO

Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana, respectively. On the basis of phylogenetic analysis based on the combined D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Lachancea fermentati CBS 707T was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene, the D1/D2 domain of the LSU rRNA gene, the ITS region, translation elongation factor-1 alpha (TEF1) and RNA polymerase II subunit 2 (RPB2) genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence, Savitreea pentosicarens gen. nov., sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is S. pentosicarens DMKU-GTCP10-8T (ex-type strain TBRC 12159=PYCC 8490; MycoBank number 835044).


Assuntos
Filogenia , Saccharomycetales/classificação , Composição de Bases , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Guiana Francesa , Genes de RNAr , Técnicas de Tipagem Micológica , Fator 1 de Elongação de Peptídeos/genética , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA , Tailândia
4.
Int J Syst Evol Microbiol ; 70(9): 4908-4913, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32716833

RESUMO

The majority of Suhomyces species have been isolated from fungus-feeding insects and particularly from the gut of beetles. In the present study, seven yeast strains were isolated from the gut of Drosophila species feeding on gleba, the spore-bearing inner mass, of a stinkhorn mushroom belonging to the family Phallaceae. Based on phenotypic, biochemical characterization and sequence analysis of the D1/D2 region of the large subunit rRNA gene and the internal transcribed spacer (ITS) region, two of these yeast strains, DGY3 and DGY4, represented a novel species of the genus Suhomyces. The novel species is closely related to an undescribed species of Candida ST-370 (DQ404513) and with Suhomyces canberraensis, wherein, the novel species differs from S. canberraensis by 40 nucleotide substitutions and three gaps (7.7 % sequence variation) in the D1/D2 region and 50 nucleotide substitutions and seven gaps (13.7 % sequence variation) in the ITS region. Several morphological and physiological differences were also observed between S. canberraensis and the strains obtained during this study. These data support the proposal of Suhomyces drosophilae as a novel species, with DGY3T as the holotype and CBS 16329T and MCC 1871T as ex-type strains.


Assuntos
Drosophila/microbiologia , Filogenia , Saccharomycetales/classificação , Agaricales , Animais , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Índia , Técnicas de Tipagem Micológica , Saccharomycetales/isolamento & purificação
6.
Int J Syst Evol Microbiol ; 70(7): 4378-4383, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32584748

RESUMO

Four isolates of two novel ascosporogenous species belonging to the clade Starmera were obtained from cactus tissues and rotting wood in Brazil. Results of analyses of the sequences of the ITS and D1/D2 domains of the large subunit rRNA gene indicated that the two isolates of the cactophilic species are related to Starmera caribaea and Starmera pilosocereana, yeasts that are associated with cacti and require an organic source of sulfur for growth. We propose the novel species Starmera foglemanii sp. nov. (CBS 16113T; MycoBank number: MB 834400) to accommodate these isolates. The other two isolates are phylogenetically related to Candida dendrica, Candida laemsonensis and Candida berthetii, also in the Starmera clade. The novel species name Starmera ilhagrandensis sp. nov. (CBS 16316T; MycoBank number: MB 834402) is proposed for this species.


Assuntos
Cactaceae/microbiologia , Filogenia , Saccharomycetales/classificação , Madeira/microbiologia , Brasil , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA
7.
Int J Syst Evol Microbiol ; 70(7): 4217-4223, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32589574

RESUMO

Five yeast strains were isolated from the gut of the groundbeetle Pterostichus gebleri and rotting wood, which were collected from two different localities in China. These strains were identified as representing two novel species of the genus Blastobotrys through comparison of sequences in the D1/D2 domains of the LSU rRNA gene and other taxonomic characteristics. Blastobotrys baotianmanensis sp. nov. produces two to three spherical ascospores per ascus, and is most closely related to the type strains of B. elegans, B. capitulata, B. arbuscula, and an undescribed species represented by strain BG02-7-20-006A-3-1. Blastobotrys baotianmanensis sp. nov. differed from these strains by 3.6-8.4 % divergence (21-46 substitutions and 0-4 gaps) in the D1/D2 sequences. Blastobotrys xishuangbannaensis f.a., sp. nov. is closely related to B. nivea, B. elegans and B. aristata but the formation of ascospores was not observed on various sporulation media, and it differed from its relatives by 6.2-8.5 % divergence (34-43 substitutions and 2-6 gaps) in the D1/D2 sequences. The holotype of Blastobotrys baotianmanensis sp. nov. is NYNU 1581 and the holotype of Blastobotrys xishuangbannaensis f.a., sp. nov. is NYNU 181030.


Assuntos
Besouros/microbiologia , Filogenia , Saccharomycetales/classificação , Madeira/microbiologia , Animais , China , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA , Esporos Fúngicos
8.
Int J Syst Evol Microbiol ; 70(5): 3379-3390, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32375944

RESUMO

During a survey of fungi in native forests in Chile, several unidentified isolates of Diaporthe were collected from different hosts. The isolates were characterized based on DNA comparisons, morphology, culture characteristics and host affiliation, in accordance with previous descriptions. Phylogenetic analysis of the ITS region, combined with partial tub2 and tef1 genes, showed that the isolates formed three distinct groups representing three new taxa. The three new species of Diaporthe, Diaporthe araucanorum on Araucaria araucana, Diaporthe foikelawen on Drimys winteri and Diaporthe patagonica on Aristotelia chilensis are described and illustrated in the present study.


Assuntos
Florestas , Filogenia , Saccharomycetales/classificação , Chile , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Genes Fúngicos , RNA Ribossômico 16S/genética , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA
9.
Int J Syst Evol Microbiol ; 70(5): 3491-3496, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32374249

RESUMO

During studies on the endophytic yeast communities associated with fruits from Vietnam, three fermenting yeast strains were isolated from fruits of the coconut palm (Cocos nucifera). Phylogenetic analysis based on the sequences of the ITS regions and D1/D2 domains of the large subunit rRNA gene showed that these strains represented a single species of the Yamadazyma clade that was distinct from the other related species. The new species represented a basal branch of the clade formed by the Yamadazyma species i.e. Y. insecticola and Y. takamatsuzukensis. Based on the phylogenetic analysis and phenotypic characteristics, the studied strains were assigned to a novel species of the genus Yamadazyma, for which the name Yamadazyma cocois f.a., sp. nov. is proposed. The holotype is VCIM 4241, with the ex-type cultures VTCC 920004=VKM Y-3049=KBP Y-6091 code 17-68. The MycoBank number is MB 834435.


Assuntos
Cocos/microbiologia , Filogenia , Saccharomycetales/classificação , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Fermentação , Técnicas de Tipagem Micológica , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA , Vietnã
10.
Cardiovasc Pathol ; 48: 107223, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32470823

RESUMO

Saprochaete species infection is a rare fungal disease reported so far only in immunocompromised patients. We describe the first case of aortitis caused by Saprochaete capitata, presenting as ascending aorta aneurysm, with secondary endophthalmitis in an immunocompetent patient. Infection by Saprochaete capitata is potentially fatal, with a mortality ranging from 50% to 90% of cases. In the present case aortic aneurysm caused by Saprochaete capitata aortitis was successfully treated by the combination of accurate diagnosis with surgical and specific antifungal therapy.


Assuntos
Aneurisma Infectado/microbiologia , Aneurisma Aórtico/microbiologia , Aortite/microbiologia , Imunocompetência , Infecções Fúngicas Invasivas/microbiologia , Revascularização Miocárdica/efeitos adversos , Saccharomycetales/isolamento & purificação , Idoso , Aneurisma Infectado/diagnóstico , Aneurisma Infectado/imunologia , Aneurisma Infectado/terapia , Antibacterianos/uso terapêutico , Aneurisma Aórtico/diagnóstico , Aneurisma Aórtico/imunologia , Aneurisma Aórtico/terapia , Aortite/diagnóstico , Aortite/imunologia , Aortite/terapia , Implante de Prótese Vascular , Humanos , Infecções Fúngicas Invasivas/diagnóstico , Infecções Fúngicas Invasivas/imunologia , Infecções Fúngicas Invasivas/terapia , Masculino , Saccharomycetales/efeitos dos fármacos , Saccharomycetales/imunologia , Resultado do Tratamento
11.
J Nat Med ; 74(3): 545-549, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32236853

RESUMO

Fungal co-culture is a strategy to induce the production of secondary metabolites by activating cryptic genes. We discovered the production of a new compound, talarodone A (1), along with five known compounds 2-6 in co-culture of Talaromyces pinophilus and Paraphaeosphaeria sp. isolated from soil collected in Miyazaki Prefecture, Japan. Among them, the productions of penicidones C (2) and D (3) were enhanced 27- and sixfold, respectively, by the co-culture. The structure of 3 should be represented as a γ-pyridol form with the reported chemical shifts, but not as a γ-pyridone form, based on DFT calculation.


Assuntos
Piridonas/metabolismo , Saccharomycetales/metabolismo , Talaromyces/metabolismo , Técnicas de Cocultura , Japão , Piridonas/química , Saccharomycetales/crescimento & desenvolvimento , Saccharomycetales/isolamento & purificação , Microbiologia do Solo , Talaromyces/crescimento & desenvolvimento , Talaromyces/isolamento & purificação
12.
Int J Syst Evol Microbiol ; 70(4): 2596-2601, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32134373

RESUMO

Seven yeast strains, DMKU VGT1-14T, DMKU VGT1-19T, DMKU-JMGT1-28, DMKU-JMGT1-32, DMKU VGT2-06, DMKU VGT2-19 and DMKU VGT6-14, were isolated from a grease trap in Thailand and two strains, SJ-1 and SN-102 were isolated from the sea surface microlayer in Taiwan. On the basis of phenotypic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, these strains represented two novel yeast species of the genus Wickerhamiella. In terms of pairwise sequence similarity, four strains, DMKU VGT1-14, DMKU-JMGT1-32, DMKU VGT6-14 and SN-102, were closely related to Wickerhamiella infanticola NRRL Y-17858T but differed by 13 nucleotide substitutions with one gap (2.46 %) in the D1/D2 domain of the LSU rRNA gene and 15 nucleotide substitutions with 23 gaps (4.2 %) in the ITS region. The strains DMKU VGT1-19T, DMKU-JMGT1-28, DMKU VGT2-06, DMKU VGT2-19 and SJ-1, differed from the type strain of the most closely related species, Wickerhamiella sorbophila NRRL Y-7921T, by nine nucleotide substitutions with one gap (1.66 %) in the D1/D2 domain of the LSU rRNA gene and nine nucleotide substitutions with 17 gaps (2.52%) in the ITS region. Hence, the names Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov. are proposed to accommodate these species in the genus Wickerhamiella. The holotypes are W. osmotolerans DMKU VGT1-14T (ex-type strain TBRC 11425=PYCC 8359=CGMCC 2.6179; Mycobank number 833394) and W. tropicalis DMKU VGT1-19T (ex-type strain TBRC 11426=PYCC 8360=CGMCC 2.6180; Mycobank number 833393).


Assuntos
Filogenia , Saccharomycetales/classificação , Água do Mar/microbiologia , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA , Taiwan , Tailândia
13.
Int J Syst Evol Microbiol ; 70(4): 2677-2681, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32207678

RESUMO

Six strains of a novel yeast species were isolated from tree bark collected in the Atlantic Forest and the Amazon Rainforest in Brazil. Analyses of the sequences of D1/D2 domains of the large subunit rRNA gene showed that the strains belong to a species in the genus Zygotorulaspora. The species differed by 5.54 % sequence divergence (25 substitutions and five indels out of 542 bp) in the D1/D2 sequences from Zygotorulaspora mrakii, its closest relative. The ITS sequence of the type strain of the novel species differs by 27-69 nucleotide substitutions/indels from the other Zygotorulaspora species. The novel species is able to grow on trehalose, maltose, l-sorbose, inulin and at 37 °C, which are negative in Z. mrakii. The name Zygotorulaspora cariocana sp. nov. is proposed. The holotype of Z. cariocana sp. nov. is CBS 16118T. The MycoBank number is MB 833702.


Assuntos
Filogenia , Casca de Planta/microbiologia , Saccharomycetales/classificação , Brasil , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Floresta Úmida , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA , Árvores/microbiologia
14.
J Agric Food Chem ; 68(8): 2418-2425, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32011876

RESUMO

Two biosynthetically related new metabolites, eucalyptacid A (1) and eucalactam B (2), along with six known compounds (3-8), eugenitol (3), cytosporone C (4), 4-hydroxyphenethyl alcohol (5), 1-(4-hydroxyphenyl)ethane-1,2-diol (6), N-(2-hydroxy-2-phenylethyl)acetamide (7), and phomopene (8), were isolated from the solid rice cultures of the endophytic fungus Diaporthe eucalyptorum KY-9 that had been isolated from Melia azedarach. Also, two further new derivatives (2a, 2b) were prepared from 2. The structures were elucidated by exhaustive analysis of NMR and ESIMS data and chemical methods such as Marfey's protocol. Compound 1 was identified as a rare polyketide fatty acid, (8E)-3,5,11-trihydroxy-2,10,12-trimethyltetradecenoic acid, and 2 was determined to be the first cyclic depsipeptide containing the same fatty acid unit as 1 and a Gly-Gly-Thr tripeptide chain. Its N-terminal end is N-acylated by an 11-hydroxy fatty acid with a branch alkyl chain of 14:1. The 11-hydroxyl group connects to the carboxylic group of the C-terminal amino acid to form a 22-membered lactone ring. A hypothetical biosynthetic pathway for the new polyketides is proposed. The isolated compounds were assayed for their inhibition against four plant pathogenic fungi, Alternaria solani, Botrytis cinerea, Fusarium solani, and Gibberella saubinettii. Compounds 1, 4, 6, and 7 exhibited antifungal activities against Alternaria solani, with minimal inhibitory concentration (MIC) values from 6.25 to 50 µM. Thus, strain KY-9 represents an untapped source for the development of biological control agents to prevent the infection of pathogenic fungus A. solani.


Assuntos
Fungicidas Industriais/metabolismo , Fungicidas Industriais/farmacologia , Melia azedarach/microbiologia , Saccharomycetales/química , Alternaria/efeitos dos fármacos , Alternaria/crescimento & desenvolvimento , Botrytis/efeitos dos fármacos , Botrytis/crescimento & desenvolvimento , Fungicidas Industriais/química , Fungicidas Industriais/isolamento & purificação , Fusarium/efeitos dos fármacos , Fusarium/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Saccharomycetales/isolamento & purificação , Saccharomycetales/metabolismo
15.
Int J Syst Evol Microbiol ; 70(4): 2420-2425, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32100691

RESUMO

Yeast strains belonging to a novel anamorphic yeast species were isolated from subsoil groundwater contaminated with hydrocarbons in a metal working factory located in northern Spain, and from a human infection in the USA. Comparison of ITS sequences between the isolates revealed 0.2 % divergence between the Spanish isolates and 0.46 % divergence between those and the USA isolate. Phylogenetic analysis based on the D1/D2 domains of the LSU rRNA gene showed that these isolates belong to the Wickerhamiella clade with W. sorbophila and W. infanticola as their closest relatives. Sequence divergence between the new isolates and W. sorbophila and W. infanticola was 1.97 and 1.79 %, respectively. The isolates in the novel species are not fermentative and pseudohyphae were not produced. Sexual reproduction was not observed for individual isolates or in mixtures of isolates. Conjugation between the isolates in the novel species and close relatives W. sorbophila and W. infanticola was not observed. These data support the proposal of Wickerhamiella verensis as a novel species, with CECT 12028T as the holotype.


Assuntos
Água Subterrânea/microbiologia , Micoses/microbiologia , Filogenia , Saccharomycetales/classificação , DNA Fúngico/genética , Fermentação , Georgia , Humanos , Hidrocarbonetos , Lactente , Técnicas de Tipagem Micológica , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA , Espanha , Poluentes Químicos da Água
16.
Yeast ; 37(4): 313-320, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32061177

RESUMO

The Wickerhamiella and Starmerella genera form a clade (W/S clade) that branches close to Yarrowia lipolytica in the Saccharomycotina species tree. It comprises approximately 90 recognized species and 50 putative new species not formally described yet. The large majority of the members of the W/S clade are ecologically associated with flowers and floricolous insects. Many species exhibit unusual metabolic traits, like fructophily and the production of sophorolipids, which are glycolipids that can be used as environmentally friendly biosurfactants. Genomic data have not only firmly established the W/S clade but have also revealed a tumultuous evolution of metabolism marked by losses and gains of important metabolic pathways, among which alcoholic fermentation. Possibly the most surprising finding brought to light by comparative genomics concerned the large number of genes acquired by some species of the W/S clade from bacteria through horizontal gene transfer, many of which were shown to be functional in their new setting. This was facilitated by the genetic tractability of one species in the clade, Starmerella bombicola, which is used for the industrial production of sophorolipids. We suggest that high-density coverage of genome sequencing in this clade, combined with the possibility to conduct molecular genetics experiments in at least one species, has the potential to set the stage for yet more exciting discoveries concerning the evolution of yeast metabolism.


Assuntos
DNA Fúngico/isolamento & purificação , Saccharomycetales/classificação , Saccharomycetales/genética , Animais , DNA Fúngico/genética , Evolução Molecular , Flores/microbiologia , Transferência Genética Horizontal , Glicolipídeos/biossíntese , Glicolipídeos/genética , Insetos/microbiologia , Redes e Vias Metabólicas , Filogenia , Saccharomycetales/isolamento & purificação
17.
Yeast ; 37(3): 253-260, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32017239

RESUMO

Species of the nitidulid beetle Conotelus found in flowers of Convolvulaceae and other plants across the New World and in Hawaii consistently harbour a yeast community dominated by one or more large-spored Metschnikowia species. We investigated the yeasts found in beetles and flowers of cultivated passionfruit in Rondônia state, in the Amazon biome of Brazil, where a Conotelus species damages the flowers and hinders fruit production. A sample of 46 beetles and 49 flowers yielded 86 and 83 yeast isolates, respectively. Whereas the flower community was dominated by Kodamaea ohmeri and Kurtzmaniella quercitrusa, the major yeasts recovered from beetles were Wickerhamiella occidentalis, which is commonly isolated from this community, and a novel species of large-spored Metschnikowia in the arizonensis subclade, which we describe here as Metschnikowia amazonensis sp. nov. Phylogenetic analyses based on barcode sequences (ITS-D1/D2) and a multigene alignment of 11,917 positions (genes ura2, msh6, and pmt2) agreed to place the new species as a sister to Metschnikowia arizonensis, a rare species known only from one locality in Arizona. The two form sterile asci when mated, which is typical of related members of the clade. The α pheromone of the new species is unique but typical of the subclade. The type of M. amazonensis sp. nov. is UFMG-CM-Y6309T (ex-type CBS 16156T , mating type a), and the designated allotype (mating type α) is UFMG-CM-Y6307A (CBS 16155A ). MycoBank MB 833560.


Assuntos
Besouros/microbiologia , Flores/microbiologia , Metschnikowia/classificação , Microbiota/fisiologia , Passiflora/microbiologia , Esporos Fúngicos , Leveduras/fisiologia , Animais , Brasil , Besouros/parasitologia , DNA Fúngico/análise , Flores/parasitologia , Metschnikowia/genética , Metschnikowia/isolamento & purificação , Metschnikowia/fisiologia , Técnicas de Tipagem Micológica , Saccharomycetales/classificação , Saccharomycetales/genética , Saccharomycetales/isolamento & purificação , Saccharomycetales/fisiologia , Alinhamento de Sequência , Análise de Sequência de DNA , Leveduras/isolamento & purificação
18.
Antonie Van Leeuwenhoek ; 113(6): 773-778, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32086682

RESUMO

Three strains originating from insect frass in South Africa, yellow foxglove in Hungary and soil in France, were characterised phenotypically and by sequencing of the D1/D2 domain of the large subunit and the ITS1-5.8S-ITS2 (ITS)-region of the rRNA gene. The strains have identical D1/D2 domain sequences and only one strain shows a 1 bp indel in a 9 bp homopolymer A/T repeat within the ITS-region. Based on sequence analysis Hyphopichia burtonii is the closest related species. The investigated strains differ from the type strain of H. burtonii by 1.9% (9 substitutions and an indel) in the D1/D2 domain and by 23 substitutions and 21-22 indels in the ITS-region. Since the sequence variability is very low among the three strains and the sequence divergence with the closely related H. burtonii exceeds the level generally encountered between species we propose the new species Hyphopichia lachancei f.a., sp. nov. to accommodate the three novel strains. From H. burtonii the new species can be distinguished phenotypically by its inability to ferment cellobiose and by the formation of endospores (Holotype: CBS 5999T; Isotype: NCAIM Y.02228T; MycoBank no.: MB833616).


Assuntos
Saccharomycetales , Animais , Celobiose/metabolismo , DNA Fúngico , DNA Ribossômico , DNA Espaçador Ribossômico/genética , Digitalis/microbiologia , Fezes/microbiologia , França , Hungria , Insetos/microbiologia , Estágios do Ciclo de Vida , Fenótipo , Filogenia , Saccharomycetales/classificação , Saccharomycetales/genética , Saccharomycetales/isolamento & purificação , Saccharomycetales/metabolismo , Microbiologia do Solo , África do Sul
19.
Int J Syst Evol Microbiol ; 70(3): 2103-2107, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31995468

RESUMO

Four strains of anamorphic yeasts isolated from the fruiting bodies of mushrooms collected in Taiwan were found to represent two novel yeast species belonging to the genus Teunomyces, which was formally known as the Candida kruisii clade. Strains NY13M09T and NY14M14 were related to the type strains of Teunomyces panamensis, T. pallodes, T. tritomae and T. lycoperdinae, and strains GG4M07T and GG6M14 were related to T. kruisii NRRL Y-17087T and T. cretensis NRRL Y-27777T. However, strains NY13M09T and NY14M14 differed from their closest phylogenetic neighbours by 2.9-3.7 % in the D1/D2 domain sequence of the LSU rRNA gene and by 6.6-13.7 % in the internal transcribed spacer (ITS); GG4M07T and GG6M14 differed from their closest known species by 2.4 % in the D1/D2 domain sequence of the LSU rRNA gene and by 8.7-10.0 % in the ITS. Meanwhile, these strains were also clearly distinguished from their closest relatives based on the results of physiological tests. Based on the characteristics described above, the strains could be regarded as representing two novel species of the genus Teunomyces, for which the names Teunomyces basidiocarpi sp. nov. and Teunomyces luguensis sp. nov. are proposed. The holotypes are Teunomyces basidiocarpi BCRC 23475T and Teunomyces luguensis BCRC 23476T.


Assuntos
Agaricales , Filogenia , Saccharomycetales/classificação , Composição de Bases , DNA Fúngico/genética , Técnicas de Tipagem Micológica , RNA Ribossômico/genética , RNA Ribossômico 16S/genética , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA , Taiwan
20.
Antonie Van Leeuwenhoek ; 113(5): 663-676, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31897851

RESUMO

Using electrophoretic karyotyping, RAPD fingerprinting and phylogenetic analysis of ribosomal RNA gene sequences, twenty-six Vanderwaltozyma strains were studied. Out of 19 strains isolated in mountainous areas of Taiwan, eighteen strains were isolated from soil and one strain was isolated from the fruiting body of mushroom, six were identified as V. polyspora and three as V. verrucispora. Based on the results of a multigene sequence analysis (D1/D2, ITS and mitochondrial COX II gene) and DNA-DNA reassociation, three new ascosporic members of the genus Vanderwaltozyma are formally described: V. huisunica sp. nov. (GA1S06T = CBS 12250T = BCRC 23260T), V. meishanica sp. nov. (EN4S02T = CBS 12249T = BCRC 23255T) and V. molinica sp. nov. (GJ8S05T = CBS 12251T = BCRC 23264T), and the holotypes of these novel species are assigned as BCRC 23260T, BCRC 23255T and BCRC 23264T, respectively.


Assuntos
Saccharomycetales , Classificação , Complexo IV da Cadeia de Transporte de Elétrons/genética , Genes Fúngicos , Genes de RNAr , Técnicas de Tipagem Micológica , Técnica de Amplificação ao Acaso de DNA Polimórfico , Saccharomycetales/classificação , Saccharomycetales/genética , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA , Microbiologia do Solo , Taiwan
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