Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.093
Filtrar
1.
Clin Oral Investig ; 28(8): 430, 2024 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-39012388

RESUMO

OBJECTIVES: This in vitro study examined the marginal integrity of experimental composite materials doped with bioactive glass (BG). MATERIALS AND METHODS: Class-II MOD cavities were prepared and restored with one of the following composite materials: a commercial composite material as a reference (Filtek Supreme XTE), an experimental composite doped with BG 45S5 (C-20), and an experimental composite doped with a fluoride-containing BG (F-20). Six experimental groups (n = 8) were used, as each of the three composites was applied with (+) or without (-) a universal adhesive (Adper Scotchbond Multipurpose). All specimens were subjected to thermocycling (10,000 x, 5-55 °C) and then additionally stored in artificial saliva for eight weeks. Scanning electron micrographs of the mesial and the distal box were taken at three time points (initial, after thermocycling, and after eight weeks of storage in artificial saliva). The margins were classified as "continuous" and "non-continuous" and the percentage of continuous margins (PCM) was statistically analyzed (α = 0.05). RESULTS: In most experimental groups, thermocycling led to a significant decrease in PCM, while the additional 8-week aging had no significant effect. F-20 + performed significantly better (p = 0.005) after 8 weeks storage in artificial saliva than the reference material with adhesive, while no statistically significant differences were observed at the other two time points. C-20 + exhibited significantly better PCM than the reference material with adhesive after thermocycling (p = 0.026) and after 8 weeks (p = 0.003). CONCLUSIONS: Overall, the experimental composites with BG showed at least as good marginal adaptation as the commercial reference, with an indication of possible re-sealing of marginal gaps. CLINICAL RELEVANCE: Maintaining or improving the marginal integrity of composite restorations is important to prevent microleakage and its likely consequences such as pulp irritation and secondary caries.


Assuntos
Resinas Compostas , Adaptação Marginal Dentária , Restauração Dentária Permanente , Vidro , Teste de Materiais , Microscopia Eletrônica de Varredura , Saliva Artificial , Propriedades de Superfície , Resinas Compostas/química , Técnicas In Vitro , Vidro/química , Restauração Dentária Permanente/métodos , Saliva Artificial/química , Humanos , Preparo da Cavidade Dentária , Cerâmica/química , Cimentos de Resina/química , Fluoretos/química
2.
Braz Oral Res ; 38: e056, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39016365

RESUMO

This study evaluated the effect of fluoride varnishes containing micrometric or nanosized sodium trimetaphosphate (TMP) on dentin erosive wear in vitro. Bovine root dentin blocks were selected by surface hardness and randomly divided into five experimental groups/varnishes (n = 20/group): placebo, 5% sodium fluoride (NaF); 5% NaF+5% micrometric TMP; 5% NaF+2.5% nanosized TMP; and 5% NaF+5% nanosized TMP. Half of the surface of all blocks received a single application of the assigned varnish, with subsequent immersion in artificial saliva for 6 h. Varnishes were then removed and the blocks were immersed in citric acid (90 s, 4×/day, 5 days). After each erosive cycle, ten blocks of each group were immersed in a placebo dentifrice for 15 s (ERO), while the other ten blocks were subjected to abrasion by brushing (ERO+ABR). Dentin erosive wear was assessed by profilometry. Data were submitted to 2-way ANOVA and to the Holm-Sidak test (p<0.05). Dentin erosive wear was significantly higher for ERO+ABR than for ERO for all varnishes. TMP-containing varnishes promoted superior effects against dentin erosive wear compared with 5% NaF alone; and 5% nanosized TMP led to the lowest wear among all varnishes. In conclusion, the addition of TMP to conventional fluoride varnish (i.e., varnish containing only NaF) enhanced its protective effects against bovine root dentin erosion and erosion+abrasion. Additionally, the use of 5% nanosized TMP led to superior effects in comparison to 5% micrometric TMP, both for erosion and erosion+abrasion in vitro.


Assuntos
Dentina , Fluoretos Tópicos , Teste de Materiais , Polifosfatos , Fluoreto de Sódio , Propriedades de Superfície , Erosão Dentária , Bovinos , Animais , Polifosfatos/farmacologia , Polifosfatos/química , Dentina/efeitos dos fármacos , Fluoreto de Sódio/farmacologia , Erosão Dentária/prevenção & controle , Fluoretos Tópicos/farmacologia , Análise de Variância , Fatores de Tempo , Propriedades de Superfície/efeitos dos fármacos , Distribuição Aleatória , Reprodutibilidade dos Testes , Nanopartículas/química , Abrasão Dentária/prevenção & controle , Saliva Artificial/química , Ácido Cítrico/farmacologia , Valores de Referência , Testes de Dureza
3.
Indian J Dent Res ; 35(1): 76-79, 2024 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-38934754

RESUMO

INTRODUCTION: Wet oral environment may have deleterious effects on performance of the composites due to influences of water sorption and solubility. The study evaluated the hydrolytic degradation caused because of water sorption and solubility of silorane and methacrylate-based dental composites. METHODS: Ten disc samples (2 mm × 10 mm) were prepared. Samples were analyzed for water solubility and sorption according to ISO 4049:2000 regulations and tested for mass gain or loss following immersion in water or in artificial saliva at 1 day, 15 days, and 30 days period. Student's 't' test, repeated measures analysis of variance (ANOVA), and Tukey's post-hoc tests determined statistical significance of the experimental results with global significance set at P = 0.05. RESULTS: Considerable sorption and solubility was observed with time in both materials on immersion. Silorane composites showed lower water sorption and solubility than methacrylate-based composite (MBC). Artificial saliva demonstrated higher sorption and solubility compared to distilled water. CONCLUSION: Silorane composites display enhanced hydrolytic stability even after a month of immersion in contrast to conventional methacrylate-based composites (MBCs), making it a better alternative to MBC resins clinically.


Assuntos
Resinas Compostas , Metacrilatos , Resinas de Silorano , Solubilidade , Água , Metacrilatos/química , Resinas Compostas/química , Água/química , Resinas de Silorano/química , Saliva Artificial/química , Teste de Materiais , Hidrólise , Materiais Dentários/química
4.
J Dent ; 147: 105120, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38857647

RESUMO

OBJECTIVES: To evaluate the wear resistance of three additively manufactured dental crown materials (NextDent C&B MFH, Saremco print CROWNTEC and Bego VarseoSmile Crown) under two environmental conditions (dry and artificial saliva), two loads (49 N and 70 N) and two surface treatments (polished and glazed). METHODS: A total of 120 specimens were divided into 24 groups and tested for wear under two loads (49 N and 70 N), surface treatment (polished or glazed), and environment (dry or submerged in artificial saliva). All samples underwent reciprocating wear testing at 1 Hz using a wear simulator, replicating 48 months of In Vivo conditions with a stainless-steel ball as the antagonist. The coefficient of friction (CoF), surface roughness, volumetric and vertical wear loss were measured and statistically analysed. Confocal microscopy assessed the surface properties of crown materials and the antagonists. RESULTS: The NextDent material demonstrated the most homogenous wear, with relatively low vertical and volumetric loss across all groups (p < 0.004). NextDent and Bego materials performed similarly in artificial saliva regardless of the load type (p > 1.000). The CoF remained below 0.3 for all groups. All groups exhibited significant increases in surface roughness after testing, however, this did not correlate with an increase in the CoF. Confocal analysis revealed material deformities due to load and notable scratch marks on the stainless-steel antagonists. CONCLUSION: It was found that all investigated addtively manufactured materials can be suggested for provisional use. Both vertical loss and volumetric loss results should be included for material evaluation. CoF and surface roughness should be implemented into wear evaluation. CLINICAL SIGNIFICANCE: This study highlights the practical value of additively manufactured dental crown materials, particularly for provisional restorations. However, their extended use requires careful consideration of individual patient needs, emphasising the need for judicious clinical application evaluation.


Assuntos
Coroas , Materiais Dentários , Desgaste de Restauração Dentária , Fricção , Teste de Materiais , Saliva Artificial , Propriedades de Superfície , Saliva Artificial/química , Materiais Dentários/química , Humanos , Polimento Dentário , Aço Inoxidável/química , Microscopia Confocal , Análise do Estresse Dentário
5.
J Dent ; 147: 105139, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38897540

RESUMO

OBJECTIVES: to investigate whether baseline mineral distribution modulates the ability of silver diammine fluoride (SDF) to remineralize and stain enamel caries lesions. METHODS: This laboratory study followed a 3 [treatment: SDF/fluoride varnish (FV)/deionized water (DIW)] ×3 [lesion protocol: methylcellulose (MeC)/hydroxyethylcellulose (HEC)/Carbopol 907 (C907)] factorial design. Lesions were created in bovine enamel specimens (n = 20). Treatments were applied and lesions remineralized in artificial saliva. Digital transverse microradiography (TMR-D) was used to analyze lesions. Lesion color was monitored spectrophotometrically. The effects of lesion protocol and treatment on changes in lesion depth (ΔLD), mineral loss (ΔΔZ), maximum mineral density at the surface zone (ΔSZmax), and color changes related to remineralization (ΔL*remin) were analyzed using two-way ANOVA. RESULTS: The treatment×lesion protocol interaction was significant for ΔΔZ (p < 0.01) and ΔL*remin (p < 0.01), however not for ΔLD (p = 0.23) or ΔSZmax (p = 0.91). There were no differences in ΔΔZ between treatments in HEC and C907 lesions. However, DIW resulted in more remineralization than both SDF (p < 0.01) and FV (p = 0.01) in MeC lesions. Considering changes from lesion baseline after remineralization in MeC lesions, SDF treatment resulted in the highest mineral gain in the surface zone. However, DIW revealed the highest mineral gain after remineralization in the lesion body. SDF stained lesions with the intensity increasing after remineralization in C907 lesions, whereas staining decreased in MeC and HEC lesions. CONCLUSION: High fluoride treatments can interfere with continuous remineralization of caries lesions due to partial arrest. Baseline lesion mineral distribution affects SDF's ability to enhance remineralization and the staining caused by SDF. CLINICAL SIGNIFICANCE: SDF is being used to arrest active caries lesions extending into dentin and to treat dentin hypersensitivity. This study shed light on SDF's effect on an isolated process in dental caries only, remineralization. It achieved this by examining enamel caries lesions with differing mineral distributions and assessing their staining properties.


Assuntos
Cariostáticos , Cárie Dentária , Esmalte Dentário , Fluoretos Tópicos , Microrradiografia , Compostos de Amônio Quaternário , Compostos de Prata , Remineralização Dentária , Animais , Remineralização Dentária/métodos , Bovinos , Cárie Dentária/tratamento farmacológico , Fluoretos Tópicos/uso terapêutico , Compostos de Prata/uso terapêutico , Compostos de Prata/farmacologia , Esmalte Dentário/efeitos dos fármacos , Esmalte Dentário/patologia , Cariostáticos/uso terapêutico , Cariostáticos/farmacologia , Compostos de Amônio Quaternário/uso terapêutico , Compostos de Amônio Quaternário/farmacologia , Metilcelulose/uso terapêutico , Resinas Acrílicas/uso terapêutico , Saliva Artificial , Minerais/análise , Minerais/uso terapêutico , Polivinil/uso terapêutico , Espectrofotometria , Água , Descoloração de Dente/tratamento farmacológico , Teste de Materiais , Celulose/análogos & derivados
6.
PLoS One ; 19(5): e0304156, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38776324

RESUMO

Saliva substitutes with enhanced dentin remineralization properties were expected to help manage caries progression in patients with xerostomia. This in vitro study examined the rheological properties and remineralization action of experimental saliva substitutes containing propolis extract and aloe vera extract on demineralized dentin. Four experimental saliva substitutes were formulated with varying concentrations of propolis extract (P) and aloe vera extract (A) were prepared. A commercial saliva substitute (Biotene Oral Rinse) was used as a commercial comparison. The rheological properties and viscosity of these materials were measured using a strain-controlled rheometer (n = 3). The remineralizing actions of saliva substitutes on demineralized dentin after 2 weeks were determined using ATR-FTIR and SEM-EDX (n = 8). The results were expressed as a percentage increase in the mineral-to-matrix ratio. Biotene demonstrated a significantly higher viscosity (13.5 mPa·s) than experimental saliva substitutes (p<0.05). The addition of extracts increased the viscosity of the saliva substitutes from 4.7 mPa·s to 5.2 mPa·s. All formulations showed minimal shear thinning behavior, which was the viscoelastic properties of natural saliva. The formulation containing 5 wt% of propolis exhibited the highest increase in the median mineral-to-matrix ratio (25.48%). The SEM-EDX analysis revealed substantial mineral precipitation in demineralized dentin, especially in formulations with 5 wt% or 2.5 wt% of propolis. The effect of the aloe vera extract was minimal. The addition of propolis and aloe vera extracts increased the viscosity of saliva substitutes. the addition of propolis for 2.5 or 5 wt% to saliva substitutes increased mineral apatite precipitation and tubule occlusion. To conclude, the saliva substitute containing propolis extract demonstrated superior remineralizing actions compared with those containing only aloe vera extract.


Assuntos
Aloe , Dentina , Extratos Vegetais , Própole , Reologia , Saliva Artificial , Própole/química , Própole/farmacologia , Aloe/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Saliva Artificial/química , Dentina/química , Dentina/efeitos dos fármacos , Humanos , Viscosidade , Remineralização Dentária/métodos , Espectroscopia de Infravermelho com Transformada de Fourier
7.
Am J Dent ; 37(2): 66-70, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38704848

RESUMO

PURPOSE: To evaluate the effect of different finishing and polishing systems on the surface roughness of a resin composite subjected to simulated saliva-, acid-, and enzyme-induced degradation. METHODS: 160 specimens (n= 40) were fabricated with Filtek Z350 XT nanofilled composite and analyzed for average surface roughness (Ra). The specimens were finished and polished using: AD - Al2O3-impreginated rubberized discs (medium, fine, and superfine grit, Sof-Lex); SD - silicon carbide and Al2O3-impregnated rubberized discs (coarse, medium and fine grit, Jiffy,); MB - 12- and 30-multiblade burs. The control group (CT) (n= 40) comprised specimens with a Mylar-strip-created surface. Specimens from each group were immersed in 1 mL of one of the degradation methods (n= 10): artificial saliva (ArS: pH 6.75), cariogenic challenge (CaC: pH 4.3), erosive challenge (ErC: 0.05M citric acid, pH 2.3) or enzymatic challenge (EzC: artificial saliva with 700 µg/mL of albumin, pH 6.75). The immersion period simulated a time frame of 180 days. Ra measurements were also performed at the post-polishing and post-degradation time points. The data were evaluated by three-way ANOVA for repeated measures and the Tukey tests. RESULTS: There was significant interaction between the finishing/polishing system and the degradation method (P= 0.001). AD presented the greatest smoothness, followed by SD. After degradation, CT, AD and SD groups became significantly rougher, but not the MB group, which presented no difference in roughness before or after degradation. CT and AD groups showed greater roughness in CaC, ErC and EzC than in ArS. The SD group showed no difference in roughness when the specimens were polished with CaC, EzC or ArS, but those treated with ErC had greater roughness. In the MB group, the lower roughness values were found after using CaC and EzC, while the higher values were found using ErC or ArS. CLINICAL SIGNIFICANCE: As far as degradation resistance of nanofilled composite to hydrolysis, bacterial and dietary acids and enzymatic reactions is concerned, restorations that had been finished and polished with Al2O3-impregnated discs had the smoothest surfaces.


Assuntos
Óxido de Alumínio , Resinas Compostas , Polimento Dentário , Saliva Artificial , Compostos de Silício , Propriedades de Superfície , Resinas Compostas/química , Polimento Dentário/métodos , Humanos , Saliva Artificial/química , Concentração de Íons de Hidrogênio , Óxido de Alumínio/química , Compostos de Silício/química , Compostos Inorgânicos de Carbono/química , Teste de Materiais , Nanocompostos/química , Ácido Cítrico/química , Saliva/enzimologia , Saliva/metabolismo , Saliva/química , Erosão Dentária , Borracha/química , Materiais Dentários/química
8.
Eur J Pharm Sci ; 199: 106802, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38763449

RESUMO

The prevalence of xerostomia, the sensation of dry mouth, is estimated at 20 % in the general population and up to 50 % in older adults. Saliva plays different roles during bolus formation: lubrication, mixing, coating, hydration, dissolution, and comminution of food particles. This study proposes and tests artificial saliva formulations mimicking human saliva rheological and sensory perceptions. Shear and extensional rheology were assessed to select the type of formulation closest to saliva rheological characteristics. After evaluating three alternative sources, an extract simulating saliva rheology was produced from flax seeds. Friction coefficient and rheological properties, such as flow curves, relaxation times, and Trouton ratios, were compared favorably with human saliva. The sensory evaluation demonstrated that flaxseed extracts induce perceived mouth hydration, slipperiness, and adhesion exceeding that of human saliva. The flaxseed extract proposed in this can i) be used to study in vitro food oral processing and ii) pave the way to novel natural salivary substitutes to alleviate the symptoms of xerostomia.


Assuntos
Linho , Reologia , Saliva Artificial , Saliva , Humanos , Saliva/química , Saliva/metabolismo , Linho/química , Saliva Artificial/química , Extratos Vegetais/química , Feminino , Adulto , Masculino , Xerostomia , Sementes/química , Adulto Jovem
9.
Arch Oral Biol ; 164: 105978, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38718465

RESUMO

OBJECTIVE: To evaluate the effect of the association of potassium iodide to antimicrobial photodynamic therapy on human carious dentin produced with a microcosm biofilm model. METHODS: A microcosm biofilm model was used to generate a caries lesion on human dentin. Pooled human saliva diluted with glycerol was used as an inoculum on specimens immersed on McBain artificial saliva enriched with 1 % sucrose (24 h at 37 °C in 5 % CO2). After refreshing culture media for 7 days, the dentin specimens were divided in 5 groups (3 specimens per group, in triplicate; n = 9): C (NaCl 0.9 %), CX (2 % chlorhexidine), PKI (0.01 % methylene blue photosensitizer+50 mM KI), L (laser at 15 J, 180 s, 22.7 J/cm2), and PKIL (methylene blue + KI + Laser). After the treatments, dentin was collected, and a 10-fold serial dilution was performed. The number of total microorganisms, total lactobacilli, total streptococci, and Streptococcus mutans was analyzed by microbial counts (CFU/mL). After normality and homoscedasticity analysis, the Welch's ANOVA and Dunnett's tests were used for CFU. All tests used a 5 % significance level. RESULTS: CX and PKIL groups showed significant bacterial decontamination of dentin, compared to group C (p < 0.05) reaching reductions up to 3.8 log10 for CX for all microorganisms' groups and PKIL showed 0.93, 1.30, 1.45, and 1.22 log10 for total microorganisms, total lactobacilli, total streptococci, and S. mutans, respectively. CONCLUSION: aPDT mediated by the association of KI and methylene blue with red laser reduced the viability of microorganisms from carious dentin and could be a promising option for cavity decontamination.


Assuntos
Biofilmes , Cárie Dentária , Dentina , Azul de Metileno , Fotoquimioterapia , Fármacos Fotossensibilizantes , Iodeto de Potássio , Streptococcus mutans , Humanos , Azul de Metileno/farmacologia , Azul de Metileno/uso terapêutico , Fotoquimioterapia/métodos , Cárie Dentária/microbiologia , Cárie Dentária/tratamento farmacológico , Cárie Dentária/terapia , Dentina/microbiologia , Dentina/efeitos dos fármacos , Iodeto de Potássio/farmacologia , Iodeto de Potássio/uso terapêutico , Biofilmes/efeitos dos fármacos , Streptococcus mutans/efeitos dos fármacos , Fármacos Fotossensibilizantes/uso terapêutico , Fármacos Fotossensibilizantes/farmacologia , Saliva/microbiologia , Lactobacillus/efeitos dos fármacos , Streptococcus/efeitos dos fármacos , Clorexidina/farmacologia , Clorexidina/uso terapêutico , Técnicas In Vitro , Contagem de Colônia Microbiana , Saliva Artificial , Lasers
10.
BMC Oral Health ; 24(1): 501, 2024 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-38725023

RESUMO

BACKGROUND: Releasing of metal ions might implicate in allergic reaction as a negative subsequent of the corrosion of Stainless Steel (SS304) orthodontic wires. The aim of this study was to evaluate the corrosion resistance of zinc-coated (Zn-coated) SS orthodontic wires. METHODS: Zinc coating was applied on SS wires by PVD method. Electrochemical impedance spectroscopy (EIS), Potentiodynamic polarization tests and Tafel analysis methods were used to predict the corrosion behavior of Zn-coated and uncoated SS wires in both neutral and acidic environments. RESULTS: The values of Ecorr ,icorr and Rct ,which were the electrochemical corrosion characteristics, reported better corrosion behavior of Zn-coated SS wires against uncoated ones in both artificial saliva and fluoride-containing environments. Experimental results of the Tafel plot analyses were consistent with that of electrochemical impedance spectroscopy analyses for both biological solutions. CONCLUSION: Applying Zn coating on bare SS orthodontic wire by PVD method might increase the corrosion resistance of the underlying stainless-steel substrate.


Assuntos
Espectroscopia Dielétrica , Teste de Materiais , Fios Ortodônticos , Saliva Artificial , Aço Inoxidável , Zinco , Corrosão , Aço Inoxidável/química , Zinco/química , Saliva Artificial/química , Ligas Dentárias/química , Materiais Revestidos Biocompatíveis/química , Fluoretos/química , Concentração de Íons de Hidrogênio , Humanos , Propriedades de Superfície , Potenciometria
11.
Appl Microbiol Biotechnol ; 108(1): 330, 2024 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-38730049

RESUMO

A more optimized culture medium used in vitro to mimic the bacterial composition of original oral flora as similar as possible remains difficult at present, and the goal of this study is to develop a novel oral biofilm medium to restore the original oral microbiome. Firstly, we conducted a systematic literature review by searching PubMed and summarized the current reported culture media in vitro. Seven culture media were found. We used mixed saliva as the origin of oral species to compare the effects of the above media in culturing oral multispecies biofilms. Results indicated that among the seven media brain heart infusion containing 1% sucrose (BHIs) medium, PG medium, artificial saliva (AS) medium, and SHI medium could obviously gain large oral biofilm in vitro. The nutrients contained in different culture media may be suitable for the growth of different oral bacteria; therefore, we optimized several novel media accordingly. Notably, results of crystal violet staining showed that the biofilm cultured in our modified artificial saliva (MAS) medium had the highest amount of biofilm biomass. 16S rRNA gene sequencing showed that the operational taxonomic units (OTUs) and Shannon index of biofilm cultured in MAS medium were also the highest among all the tested media. More importantly, the 16S rRNA gene sequencing analysis indicated that the biofilm cultured in MAS medium was closer to the original saliva species. Besides, biofilm cultured by MAS was denser and produced more exopolysaccharides. MAS supported stable biofilm formation on different substrata. In conclusion, this study demonstrated a novel MAS medium that could culture oral biofilm in vitro closer to the original oral microbiome, showing a good application prospect. KEY POINTS: • We compare the effects of different media in culturing oral biofilms • A novel modified artificial saliva (MAS) medium was obtained in our study • The MAS medium could culture biofilm that was closer to oral microbiome.


Assuntos
Bactérias , Biofilmes , Meios de Cultura , Microbiota , Boca , RNA Ribossômico 16S , Saliva , Humanos , Bactérias/genética , Bactérias/classificação , Bactérias/isolamento & purificação , Biofilmes/crescimento & desenvolvimento , Meios de Cultura/química , Boca/microbiologia , RNA Ribossômico 16S/genética , Saliva/microbiologia , Saliva Artificial
12.
BMC Oral Health ; 24(1): 643, 2024 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-38822326

RESUMO

OBJECTIVES: Since maintaining oral hygiene is essential in nursing care, the present study was conducted to determine the effect of oral care using Mucosamin artificial saliva spray to control dry mouth in ICU patients with COVID-19. MATERIALS AND METHODS: The current semi-experimental research was conducted on eighty patients with COVID-19 selected using the available sampling method. The study tool was a Beck oral assessment scale (BOAS). The case and control groups were selected from two hospitals with relatively similar conditions and treatment procedures. For patients in the intervention group, mucosamin artificial saliva spray was used in addition to the common care, while control group patients received only common care. RESULTS: Eighty patients were randomly assigned to two groups named control and intervention (40 patients in each group). The intervention was very effective in reducing the BOAS score after four days in comparison with the control group (9.23 vs. 12.05, respectively; p-value < 0.001). Based on the adjusted model, the application of artificial saliva reduced the BOAS score, indicating improvement in mouth dryness. While the BOAS score was increased in the control group, it had a declining trend in the intervention one. CONCLUSION: The study's results showed that using artificial saliva spray could effectively reduce the symptoms of dry mouth in patients with COVID-19 treated with non-invasive mechanical ventilation. CLINICAL RELEVANCE: The present study introduced an applicable solution (artificial saliva) to treat mouth dryness in ICU patients under mechanical ventilation.


Assuntos
COVID-19 , Respiração Artificial , Saliva Artificial , Xerostomia , Humanos , Saliva Artificial/uso terapêutico , Xerostomia/terapia , COVID-19/prevenção & controle , Feminino , Masculino , Pessoa de Meia-Idade , Respiração Artificial/métodos , Adulto , Idoso , SARS-CoV-2 , Higiene Bucal/métodos
13.
Eur Arch Paediatr Dent ; 25(3): 385-392, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38664350

RESUMO

OBJECTIVE: Colour stability is important in the long-term aesthetic success of restorative materials and is affected by both internal and external factors. Internal discolourations are due to the properties of the restorative materials. External discolourations can be associated with frequent consumption of food and beverages and the use of suspensions or syrups containing colourants/additives. Fluoride varnish application has an important place in preventive dentistry. The purpose of the research was to examine the protective effect of fluoride varnish application on the colour change on polyacid-modified composite resin restorative materials caused by the use of various paediatric drugs. METHODS: Two hundred ten discs were prepared from polyacid-modified composite resin material and divided into two groups: flouride varnish was applied to one group and flouride varnish was not applied to the other group. The groups were further divided into seven subgroups and the samples were kept in artificial saliva, amoxicillin + clavulanic acid, cefuroxime axetil, clarithromycin, paracetamol, ibuprofen, and iron supplement drug solutions. The colour change values of the discs were measured using a spectrophotometer device before immersion in the drug solutions and on the 7th, 14th, 21st, and 28th days after the immersion. The obtained data were calculated and statistically evaluated using IBM SPSS V23 software. RESULTS: It was found that the application of fluoride varnish in the iron supplement drug group prevented the colour change of the polyacid-modified composite resins for 28 days. In the amoxicillin + clavulanic acid, cefuroxime axetil, and paracetamol groups, the fluoride varnish did not prevent colour change in the polyacid-modified composite resin restorative materials at the end of the 14th day. CONCLUSION: It is thought that fluoride varnish application may be beneficial as a preservative in the colour change of polyacid-modified composite resins due to the use of various paediatric drugs, and this protective feature may be effective for a specific period of time.


Assuntos
Cor , Resinas Compostas , Fluoretos Tópicos , Resinas Compostas/química , Fluoretos Tópicos/química , Humanos , Técnicas In Vitro , Teste de Materiais , Espectrofotometria , Saliva Artificial/química , Fluoreto de Sódio/química , Materiais Dentários/química
14.
J Esthet Restor Dent ; 36(7): 1056-1067, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38581272

RESUMO

OBJECTIVE: To evaluate the effect of fluoride consistency and composition to protect enamel and dentin against the dental erosion. MATERIALS AND METHODS: Bovine enamel and dentin specimens were treated with artificial saliva, neutral fluoride gel (NFG), acidulated phosphate fluoride gel (AFG), neutral fluoride foam (NFF), and acidulated phosphate fluoride foam. The samples were subjected to cycling. Micro energy-dispersive X-ray fluorescence spectrometry, surface roughness (Ra), contact angle (CA), and scanning electron microscopy (SEM) were performed. Composition, CA and Ra data were analyzed by ANOVA and multiple comparison test (p < 0.05). RESULTS: The dentin protected had a significantly higher mineral content than in the control. Eroded unprotected enamel had higher Ra values than normal surfaces. Fluoride treatments increased the Ra in dentin samples. AFG increased the CA in enamel. Fluoride foams increased CA in dentin with reduced mineral loss. SEM analysis found a deposited layer on enamel treated with AFG and remnants of deposits on dentin treated with NFG and NFF. CONCLUSION: Regardless of the form of application, fluoride provided protection against erosion, however with different levels. CLINICAL SIGNIFICANCE: Applying the adequate fluoride form is relevant since the formulations have different effects on both enamel and dentin.


Assuntos
Esmalte Dentário , Dentina , Fluoretos , Microscopia Eletrônica de Varredura , Espectrometria por Raios X , Propriedades de Superfície , Erosão Dentária , Animais , Erosão Dentária/prevenção & controle , Bovinos , Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Fluoretos/farmacologia , Fluoreto de Fosfato Acidulado/farmacologia , Saliva Artificial , Fluoretos Tópicos/farmacologia
15.
Folia Med (Plovdiv) ; 66(1): 104-113, 2024 Feb 29.
Artigo em Inglês | MEDLINE | ID: mdl-38426472

RESUMO

INTRODUCTION: The worldwide application of digital technology has presented dentistry with transformative opportunities. The concept of digital dentures, incorporating computer-aided design (CAD) and computer-aided manufacturing (CAM) techniques, holds the promise of improved precision, customization, and overall patient satisfaction. However, the shift from traditional dentures to their digital counterparts should not be taken lightly, as the intricate interplay between oral physiology, patient comfort, and long-term durability requires thorough examination.


Assuntos
Bases de Dentadura , Imersão , Humanos , Saliva Artificial , Desenho Assistido por Computador , Impressão Tridimensional
16.
Artigo em Inglês | MEDLINE | ID: mdl-38452631

RESUMO

This study aimed to prove the validity of a mixture of chemicals, including salts, small organic molecules, mucin, and α-amylase, as saliva surrogate ("artificial saliva") for assessing leakage of methacrylate monomers and other constituents from dental materials. To achieve this, we developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 2-hydroxyethyl methacrylate (HEMA), triethylene glycol dimethacrylate (TEGDMA), diurethane dimethacrylate (UDMA), bisphenol A glycerolate dimethacrylate (BisGMA), diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide (TPO), bisphenol A (BPA), and five homologues of ethoxylated bisphenol A dimethacrylate (BisEMA EO2-6) in unstimulated and artificial saliva, and compared their concentrations in the two saliva media following either spiking with a mixture of the compounds or incubation of test specimens of printed biomaterials. Test specimens were immersed in unstimulated/artificial saliva, incubated at 37 °C for 24 h, and saliva aliquots were extracted with methanol and subsequently analyzed by LC-MS/MS. The method was validated with regard to matrix effects, linearity, selectivity, lower limits of quantification (LLOQ), precision, bias and combined measurement uncertainty (u'). The performance characteristics of the method were comparable for unstimulated and artificial saliva samples. The combined u' for individual chemicals at a concentration of 10 × LLOQ were within the range of 5.3-14 % for unstimulated saliva and 6.9-16 % for artificial saliva, except for the BisEMA homologues. Combined u' for the latter were 27-74 % in unstimulated saliva, and 27-79 % in artificial saliva. There was no detectable release of BPA from the test specimens, and the TPO concentrations were mainly below the LLOQ. TEGDMA and UDMA were detected in the highest quantities, and at comparable concentrations in the unstimulated and artificial saliva. For all BisEMA homologues, the release was higher in unstimulated saliva than in artificial saliva. The study showed that the artificial saliva model can be a suitable replacement for native saliva, but might underestimate leakage of more lipophilic methacrylates.


Assuntos
Compostos Benzidrílicos , Resinas Compostas , Fenóis , Saliva , Humanos , Saliva/química , Cromatografia Líquida , Resinas Compostas/análise , Resinas Compostas/química , Saliva Artificial/análise , Espectrometria de Massas em Tandem , Metacrilatos/química , Ácidos Polimetacrílicos/química , Polietilenoglicóis/química , Teste de Materiais
17.
Food Res Int ; 182: 114156, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38519183

RESUMO

Food texture perception is dynamic, influenced by food properties and oral processing. Using the Repeatable Dual Extrusion Cell (RDEC), the oral processing dynamics of surimi gel with different corn starch concentrations (0-15%) in the presence of 1 ml artificial saliva or water were studied. The force-time curve showed increased peak forces with higher corn starch concentrations, peaking significantly at 10%, then decreasing at 15%. Salivary amylase played a crucial role in gel sample degradation, especially in samples with 5% starch, with a work value depletion ratio of 0.535 for sample with 1 ml water (SGW-5) and 0.406 for sample with 1 ml saliva (SGS-5). SEM analysis confirmed the formation of a continuous starch network with reduced intermolecular spaces in SGS-5. The starch-iodine complex showed decreasing order with increasing starch concentration, and SGS-5 exhibited the highest degradation rate (61.61 ± 0.92%). Mathematical modeling revealed that initial decay rates (k1) in gel sample decreased with increasing starch concentration, and samples with starch and artificial saliva had higher initial degradation rates. These findings highlight the intricate interplay between saliva and starch in the surimi gel matrix under continuous compressive motions by RDEC apparatus, providing insights for formulating food products with tailored textures properties.


Assuntos
Saliva , Amido , Amido/química , Saliva Artificial , Saliva/metabolismo , Géis/química , Água
18.
Clin Oral Investig ; 28(3): 185, 2024 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-38429372

RESUMO

OBJECTIVE: To compare four commercially available Essix-type retainers in terms of longevity, wear characteristics, stiffness and their range of rigidity. MATERIALS AND METHODS: An in vitro study was conducted at Queen Mary University of London. Four groups of thermoplastic materials were included: Duran (PETG), Essix C + (Polypropylene), Vivera and Zendura (Polyurethane). A working typodont was fabricated to evaluate surface wear characteristics using a wear machine with a customized jig. Retainers were measured for tensile test, and water absorption was measured at five different time points up to 6 months after initial immersion in two different physical states and two different solutions. Hydrolytic degradation was also evaluated using FTIR spectroscopy. RESULTS: Essix C + was the most flexible retainer with Vivera the stiffest material. Zendura and Essix C + had the most surface wear (413 µm ± 80 and 652 µm ± 12, respectively) with absorption rates of up to 15 wt% in artificial saliva occurring with Zendura. Only Essix C + displayed signs of degradation following water absorption. CONCLUSIONS: All materials had characteristic levels of flexibility and were susceptible to water absorption. Duran 1.5 mm performed similarly to Vivera in relation to stiffness and wear properties. While Zendura and Vivera have similar chemical structures, they exhibited differences concerning wear resistance and water absorption. Further clinical research evaluating the clinical relevance of these laboratory findings is required. CLINICAL RELEVANCE: Characteristic patterns of wear and rigidity of four commercially available Essix-type retainers were observed. This information should help in the tailoring of retainer material on a case-by-case basis considering treatment-related factors and patient characteristics including parafunctional habits.


Assuntos
Longevidade , Polipropilenos , Humanos , Poliuretanos , Saliva Artificial , Água
19.
Dent Mater J ; 43(2): 269-275, 2024 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-38417859

RESUMO

We aimed to improve the biocompatibility and osteoinductive potential of Ti implants using a simulated intraoral hydroxyapatite (HAp) coating. We devised a novel surface treatment for aggressive induction of osteoblast adhesion and bone regeneration on the implant surface. A thin α-tricalcium phosphate (α-TCP) film was deposited on the implant surface using a pulsed Er:YAG laser. The coating was converted to HAp through artificial saliva immersion, which was confirmed using scanning electron microscopy (SEM) and X-ray diffraction (XRD). SEM showed needle-like HAp crystals on the Ti disks and sandblasted implant surfaces after immersion in artificial saliva for 96 h. Microcomputed tomography and histological evaluation 4 and 8 weeks after implantation into beagle dog mandibles showed that the HAp-coated implant was biocompatible and exhibited superior osteoinduction compared to that of sandblasted implants. Coating the implant surface with HAp using an Er:YAG laser has potential as a new method of the implant-surface debridement.


Assuntos
Implantes Dentários , Lasers de Estado Sólido , Cães , Animais , Durapatita/farmacologia , Durapatita/química , Saliva Artificial , Microtomografia por Raio-X , Materiais Revestidos Biocompatíveis/farmacologia , Materiais Revestidos Biocompatíveis/química , Titânio/farmacologia , Titânio/química , Microscopia Eletrônica de Varredura , Propriedades de Superfície
20.
Clin Oral Investig ; 28(2): 132, 2024 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-38308668

RESUMO

OBJECTIVES: This study aimed at comparing the microtensile bond strength (MTBS) and interfacial adaptation of a modern self-curing and a light-curing restorative bulk-fill composite to a conventional composite applied with the layering technique. METHODS: Forty-eight occlusal cavities were divided in three main groups (16/group) based on tested materials: (i) STELA, bulk-fill self-curing restorative (STELA, SDI Ltd.); (ii) 3 M-BULK, bulk-fill composite (Filtek One Bulk-Fill, 3 M Oral Care); and (iii) 3 M-CTR, a conventional composite (Filtek Supreme XTE, 3 M Oral Care). These were used in combination with their adhesives in self-etch (SE) or etch-and-rinse (ER) mode. Specimens stored in artificial saliva (24 h or 12 months) were evaluated for MTBS and fractography. The interfacial analysis was performed through confocal microscopy. ANOVA and Fisher's LSD post hoc tests were performed with a level of significance of 5%. RESULTS: All the tested materials applied in ER mode presented (24 h) greater bond strength than in SE mode. Although all materials showed a significant drop in the bond strength after prolonged storage, STELA showed the highest bonding performance and interfaces with few gaps. 3 M-BULK had the lowest bond strength and an interface with several voids and gaps. CONCLUSIONS: All materials were affected by interface degradation and bonding reduction over prolonged aging. However, their use in combination with adhesives applied in ER mode may offer greater immediate bonding performance. CLINICAL RELEVANCE: The use of restorative light-curing bulk-fill composites may generate gaps at the bonding interface and voids. STELA may represent a suitable alternative to avoid such issues.


Assuntos
Colagem Dentária , Cimentos de Resina , Saliva Artificial , Cimentos de Resina/química , Resinas Compostas/química , Teste de Materiais
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA