Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 40.782
Filtrar
1.
Am J Orthod Dentofacial Orthop ; 157(3): 385-391, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32115117

RESUMO

INTRODUCTION: Orthodontic patients are at an increased risk for developing caries. Dental caries is a biofilm-mediated disease, with mutans streptococci (MS) as the primary etiologic bacterial group. It has been suggested that persister cells (PCs), a subset of cells within the biofilm, contribute to the chronic infectious nature of dental caries. PC formation can be induced by environmental stressors such as orthodontic treatment. The aim of this study was to quantify MS, aerobic and facultative anaerobe bacterial PC proportions from plaque samples during the initial stage of orthodontic treatment. This study is the first to analyze the role of PCs in a population of patients highly susceptible to caries, that is, patients undergoing orthodontic treatment. METHODS: Plaque samples were collected from 17 participants (11 males and 6 females; age range: 11-18 years) before and 1 month after insertion of fixed orthodontic appliances. Percentages of MS and PCs were determined with selective media and a classical persister microbial assay, respectively. RESULTS: There was a statistically significant decrease in %MS (P = 0.039) but no statistically significant difference in %PCs (P = 0.939) after 1 month of orthodontic appliance placement. CONCLUSION: Our study illustrated the technical feasibility of analysis of PCs in plaque samples of patients during orthodontic treatment and revealed that PC formation during orthodontic treatment is highly variable across individuals.


Assuntos
Cárie Dentária , Placa Dentária , Aparelhos Ortodônticos Fixos , Streptococcus mutans , Adolescente , Criança , Cárie Dentária/microbiologia , Feminino , Humanos , Masculino , Aparelhos Ortodônticos , Aparelhos Ortodônticos Fixos/microbiologia , Saliva , Streptococcus mutans/isolamento & purificação
2.
Eur J Paediatr Dent ; 21(1): 13-17, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32183522

RESUMO

AIM: Oral streptococci were found to be associated with Early Childhood Caries. The aim of this study was to evaluate the 6 different bacteria in the streptococcus group in the saliva of children with severe early childhood caries (S-ECC) by polymerase chain reaction (PCR). MATERIALS AND METHODS: A total of 60 children between 3 and 6 years of age were divided into two groups: children with S-ECC (Group S-ECC; n=30) and children who were caries-free (Group CF; n=30), according to the dmft and dmfs indices. Unstimulated saliva was collected from all participants for the detection of streptococcal group bacteria, including: Streptococcus mutans, Streptococcus oralis, Streptococcus sanguinis, Streptococcus gordonii, Streptococcus salivarius, and Streptococcus sobrinus, using PCR-restriction fragment length polymorphism (RFLP) of amplified 16S rRNA gene. The data were analysed using SPSS software. RESULTS: The prevalence of S. oralis was significantly higher in the S-ECC group compared to the CF group (p<0.05). However, the frequencies of S. mutans, S. sanguinis, S. gordonii, S. salivarius, and S. sobrinus were similar between the two groups (p>0.05). The amount of streptococci colonies was higher in the S-ECC group compared to the CF group (p<0.05). CONCLUSION: S. mutans or S. sobrinus alone may not be the only indicators for high risk of caries, but the prevalence of S. oralis in saliva may be a risk factor for increased caries activity in S-ECC.


Assuntos
Cárie Dentária , Saliva , Criança , Pré-Escolar , Humanos , RNA Ribossômico 16S , Streptococcus , Streptococcus mutans , Streptococcus sobrinus
3.
Medicine (Baltimore) ; 99(8): e19097, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32080083

RESUMO

Liquid biopsy is an emerging technique for noninvasive detection of various cancers. Majority of liquid biopsy tests still, however, use solitary type of biomarkers with unsatisfactory sensitivity and specificity. To this end, a combined approach of circulating tumor cells (CTCs) and salivary mRNA biomarkers was evaluated for discriminating non-small-cell lung cancer (NSCLC) from healthy controls.Our study included a discovery phase to find multiple biomarkers, and an independent validation phase to confirm the applicability of the selected biomarkers. In the discovery phase, CTC level in blood and 5 mRNA biomarkers in saliva (i.e., CCNI, Epidermal growth factor receptor [EGFR], FGF19, FRS2, and GREB1) were measured for 140 NSCLC patients and 140 healthy controls, followed by developing a predictive model. Next, this panel of biomarkers was applied to another patient cohort consisted of 60 patients with NSCLC and 60 healthy controls in the validation phase.We found that our novel biomarker panel could differentiate patients with NSCLC from healthy controls with high sensitivity (92.1%) and high specificity (92.9%) in the discovery phase. In the validation phase, we achieved sensitivity of 88.3% and specificity of 90.0%.To our best knowledge, it is the first time that a combined use of CTC and salivary mRNA biomarkers were applied for noninvasive detection of NSCLC.


Assuntos
Biomarcadores Tumorais/sangue , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Células Neoplásicas Circulantes/metabolismo , RNA Mensageiro/metabolismo , Saliva/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Adulto , Idoso , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Ciclina I/metabolismo , Receptores ErbB/metabolismo , Feminino , Fatores de Crescimento de Fibroblastos/metabolismo , Humanos , Biópsia Líquida/métodos , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Masculino , Proteínas de Membrana/metabolismo , Pessoa de Meia-Idade , Proteínas de Neoplasias/metabolismo , Células Neoplásicas Circulantes/patologia , Valor Preditivo dos Testes , Sensibilidade e Especificidade
4.
Emerg Microbes Infect ; 9(1): 496-504, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32106784

RESUMO

The mesoniviruses (MESOVs) belong to the newly described Mesoniviridae family (Order: Nidovirales). They have never been reported in Senegal until recently during a study in arbovirus emergence with the detection of a new species of MESOV named Dianke virus (DKV) from common mosquitoes from eastern Senegal. Actually, their vector competence for this newly described DKV is unknown. We, therefore, estimated the vector competence of Culex tritaeniorhynchus, Culex quinquefasciatus, Aedes aegypti, and Anopheles gambiae mosquitoes collected in Senegal for DKV using oral infection. Whole bodies, legs/wings, and saliva samples were tested for DKV by RT-PCR to estimate infection, dissemination, and transmission rates. The infectivity of virus particles in the saliva was confirmed by infecting C6/36 cells. Virus transmission rates were up to 95.45% in Culex tritaeniorhynchus, 28% in Cx. quinquefasciatus and 9.09% in Aedes aegypti. Viral particles in the saliva were confirmed infectious by C6/36 cell culture. An. gambiae was able to disseminate DKV only at 20 days post-infection. This study shows that Culex mosquitoes are more competent than Ae. aegypti for DKV, while Anopheles gambiae is not likely a competent vector.


Assuntos
Aedes/virologia , Mosquitos Vetores , Nidovirales , Animais , Linhagem Celular , Feminino , Infecções por Nidovirales/transmissão , Saliva/virologia , Senegal
5.
Sud Med Ekspert ; 63(1): 42-46, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32040087

RESUMO

The purpose of the study is to develop a method for detecting, isolating and quantifying lamivudine in biological substances. Lamivudine was isolated by liquid-liquid and solid phase extraction. The conditions for isolating lamivudine (extractant, pH of the medium, electrolyte, time and frequency of extraction) from aqueous solutions were selected and methods were developed for isolating it from biological substances, including urine, saliva and liver, using liquid-liquid and solid phase extraction methods. Qualitative and quantitative analysis of lamivudine in extracts from urine, saliva and liver was performed by thin layer chromatography, UV spectrophotometry and high-performance liquid chromatography. A validation assessment of the developed techniques indicates their suitability for chemical and toxicological analysis of lamivudine.


Assuntos
Lamivudina/análise , Fígado/química , Saliva/química , Urinálise , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Delgada , Humanos , Extração em Fase Sólida , Espectrofotometria
6.
BMC Med Genet ; 21(1): 34, 2020 02 14.
Artigo em Inglês | MEDLINE | ID: mdl-32059710

RESUMO

BACKGROUND: Epigenetics could facilitate greater understanding of disparities in the emergence of childhood obesity. While blood is a common tissue used in human epigenetic studies, saliva is a promising tissue. Our prior findings in non-obese preschool-aged Hispanic children identified 17 CpG dinucleotides for which differential methylation in saliva at baseline was associated with maternal obesity status. The current study investigated to what extent baseline DNA methylation in salivary samples in these 3-5-year-old Hispanic children predicted the incidence of childhood obesity in a 3-year prospective cohort. METHODS: We examined a subsample (n = 92) of Growing Right Onto Wellness (GROW) trial participants who were randomly selected at baseline, prior to randomization, based on maternal phenotype (obese or non-obese). Baseline saliva samples were collected using the Oragene DNA saliva kit. Objective data were collected on child height and weight at baseline and 36 months later. Methylation arrays were processed using standard protocol. Associations between child obesity at 36 months and baseline salivary methylation at the previously identified 17 CpG dinucleotides were evaluated using multivariable logistic regression models. RESULTS: Among the n = 75 children eligible for analysis, baseline methylation of Cg1307483 (NRF1) was significantly associated with emerging childhood obesity at 36-month follow-up (OR = 2.98, p = 0.04), after adjusting for child age, gender, child baseline BMI-Z, and adult baseline BMI. This translates to a model-estimated 48% chance of child obesity at 36-month follow-up for a child at the 75th percentile of NRF1 baseline methylation versus only a 30% chance of obesity for a similar child at the 25th percentile. Consistent with other studies, a higher baseline child BMI-Z during the preschool period was associated with the emergence of obesity 3 years later, but baseline methylation of NRF1 was associated with later obesity even after adjusting for child baseline BMI-Z. CONCLUSIONS: Saliva offers a non-invasive means of DNA collection and epigenetic analysis. Our proof of principle study provides sound empirical evidence supporting DNA methylation in salivary tissue as a potential predictor of subsequent childhood obesity for Hispanic children. NFR1 could be a target for further exploration of obesity in this population.


Assuntos
Biomarcadores/metabolismo , Metilação de DNA/genética , Epigênese Genética , Obesidade Pediátrica/genética , Adulto , Índice de Massa Corporal , Pré-Escolar , Ilhas de CpG/genética , Feminino , Humanos , Masculino , Obesidade Pediátrica/diagnóstico , Obesidade Pediátrica/fisiopatologia , Gravidez , Saliva/metabolismo
7.
Neurol Neurochir Pol ; 54(1): 14-20, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32003440

RESUMO

The identification of reliable biomarkers of Parkinson's disease (PD) is a pivotal step in the introduction of causal therapies. Saliva is a biofluid which may be involved in synuclein pathology in PD. We have reviewed current studies on salivary proteins and compounds in PD patients and healthy controls, and their potential application as biomarkers. A systematic literature search of the Pubmed and Scopus databases was performed. A total of 198 studies were screened, of which 20 were included in our qualitative analysis. We conclude that the oligomeric form of salivary alpha synuclein is higher in PD patients, and that this may serve as a potential biomarker of PD. Salivary DJ-1 concentrations fail to differentiate PD patients from controls. Other enzymes and substances (heme oxygenase-1, nitric oxide, acetylcholinesterase) have been assessed in single studies. Salivary cortisol levels are higher in PD than in healthy subjects. Further validation of these findings is needed. Saliva may be a promising source of biomarkers in PD.


Assuntos
Doença de Parkinson , Biomarcadores , Humanos , Boca , Saliva , alfa-Sinucleína
8.
Braz Oral Res ; 34: e002, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32049107

RESUMO

Oral potentially malignant disorders (OPMD) possess significant chances of malignancy conversion. In order to develop an early diagnostic tool, the present study evaluated the expression of miRNA-21 and 31 as salivary markers. The case-control study was carried out in 36 healthy participants as controls and in 36 patients who were newly diagnosed as OPMD having four different lesions including leucoplakia, oral sub mucous fibrosis (OSMF)궱, oral lichen planus, and (OSMF)궱 with leucoplakia. The samples were also classified as non-dysplastic, or with mild, moderate, and severe dysplasia according to their histopathological reports. The salivary miRNA-21 and 31 expressions were studied using real-time PCR. The statistical analysis was carried out using SPSS version 22. Salivary miRNA-21 (p-value = 0.02) and 31 (p-value = 0.01) were significantly upregulated in severe dysplasia compared with control. Among the different lesions, leucoplakia had significant upregulation of miRNA-21 and 31. miRNA-21 can be used as a diagnostic marker with specificity of 66% and sensitivity of 69%. The area under the ROC curve was 0.820 for miRNA-21 and 0.5 for miRNA-31, which proved that miRNA-21 is a better diagnostic marker than miRNA-31 for OPMD.


Assuntos
MicroRNAs/análise , Neoplasias Bucais/patologia , Lesões Pré-Cancerosas/patologia , Saliva/química , Análise de Variância , Biomarcadores Tumorais/análise , Estudos de Casos e Controles , Humanos , Leucoplasia Oral/patologia , Líquen Plano Bucal/patologia , Modelos Lineares , Neoplasias Bucais/química , Fibrose Oral Submucosa/patologia , Curva ROC , Reação em Cadeia da Polimerase em Tempo Real , Valores de Referência , Índice de Gravidade de Doença
9.
Medicine (Baltimore) ; 99(6): e19098, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32028434

RESUMO

Cortisol is the main end product of hypothalamic-pituitary-adrenal gland (HPA axis), and melatonin (MT) has a regulating effect on HPA axis, and both are closely related to individual behavior and cognitive function. We aimed to evaluate cortisol and MT roles on children dyslexia in this study.A total of 72 dyslexic children and 72 controls were recruited in this study. Saliva samples were collected in the morning, afternoon, and night, respectively. The levels of saliva cortisol and MT were measured by enzyme-linked immunosorbent assay method. Differences of cortisol and MT levels between dyslexic and normal children were compared, and the variation trend was also analyzed by dynamic monitoring in 3 time points.The levels of salivary cortisol and MT in children with dyslexia were all lower than those in normal children whether in the morning (7:30-8:30 AM ), at afternoon (15:30-16:30 PM ) or at night (21:30-22:30 PM ) (all P < .001). Compared with normal children, the circadian rhythm variations of salivary cortisol and MT in dyslexic children disappeared and became disordered. The salivary cortisol and MT levels in children with dyslexia were declined throughout the day; and the circadian rhythm was disordered or disappeared.The results suggest that cortisol and MT levels and their circadian rhythm may affect children dyslexia, but the mechanisms need further exploration.


Assuntos
Transtornos Cronobiológicos/metabolismo , Dislexia/metabolismo , Hidrocortisona/análise , Melatonina/análise , Saliva/química , Estudos de Casos e Controles , Criança , China , Transtornos Cronobiológicos/complicações , Ritmo Circadiano , Dislexia/complicações , Feminino , Humanos , Masculino
10.
Med Oral Patol Oral Cir Bucal ; 25(2): e299-e310, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32040469

RESUMO

BACKGROUND: Saliva evaluation could be a possible alternative to blood and/or tissue analyses, for researching specific molecules associated to the presence of systemic diseases and malignancies. The present systematic review has been designed in order to answer to the question "are there significant associations between specific salivary biomarkers and diagnosis of systemic diseases or malignancies?". MATERIALS AND METHODS: The Preferred Reporting Item for Systematic Reviews and Meta-analysis (PRISMA) statement was used to guide the review. The combinations of "saliva" and "systemic diseases" or "diagnosis" or "biomarkers" or "cancers" or "carcinoma" or "tumors", were used to search Medline, Scopus and Web of Science databases. Endpoint of research has been set at May 2019. Studies were classified into 3 groups according to the type of disease investigated for diagnosis: 1) malignant tumors; 2) neurologic diseases and 3) inflammatory/metabolic/cardiovascular diseases. Assessment of quality has been assigned according to a series of questions proposed by the National Institute of Health. Level of evidence was assessed using the categories proposed in the Oxford Center for Evidence-Based medicine (CEMB) levels for diagnosis (2011). RESULTS: Seventy-nine studies met the inclusion and exclusion criteria. Fifty-one (64%) investigated malignant tumors, 14 (17.5%) neurologic and 14 (18.5%) inflammatory/cardiovascular/metabolic diseases. Among studies investigating malignant tumors, 12 (23.5%) were scored as "good" and 11 of these reported statistically significant associations between salivary molecules and pathology. Two and 5 studies were found to have a good quality, among those evaluating the association between salivary biomarkers and neurologic and inflammatory/metabolic/cardiovascular diseases, respectively. CONCLUSIONS: The present systematic review confirms the existence of some "good" quality evidence to support the role of peculiar salivary biomarkers for diagnosis of systemic diseases (e.g. lung cancer and EGFR).


Assuntos
Doenças Cardiovasculares , Neoplasias , Biomarcadores , Humanos , Saliva
11.
Anim Cogn ; 23(2): 389-403, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31907679

RESUMO

Rescue behavior is considered a type of pro-social response, defined as a voluntary action directed to benefit another individual who is in a stressful or dangerous situation. In two experiments, we investigated whether dogs would rescue their owners when the person was trapped inside a wooden box and emitted clear signs of stress. The performance of these dogs was compared against that of a control group in which the owners remained calm while trapped. In addition, to assess if training modulated this behavior, we tested a group of dogs from the military trained in search and rescue tasks (Experiment 1). Results showed that dogs opened the box more frequently when the owner pretended to be stressed than when calm. Training shortened latencies to open the door but not the frequency of the behavior. In Experiment 2, we investigated if emotional contagion could be a possible mechanism underlying dogs' rescue responses by measuring dogs' behavior, heart rate, and saliva cortisol level in the stressed and calm conditions, and also controlled for obedience by having the calm owners call their pets while trapped. We replicated the findings of Experiment 1 as more dogs opened the door in the stressed owner condition than in the calm condition. In addition, we observed an increase in heart rate across trials in the stressed condition and a decrease across trials in the calm condition, but no differences in cortisol levels or stress-related behaviors between conditions. In brief, we found evidence that approximately half of the dogs without previous training showed spontaneous rescue behaviors directed to their owners. Neither was this behavior motivated by obedience nor by the motivation to re-establish social contact with the owner. We conclude that emotional contagion is a plausible mechanism underlying dogs' rescue behavior in the present protocol.


Assuntos
Comportamento Animal , Vínculo Homem-Animal de Estimação , Estresse Psicológico , Animais , Comportamento Animal/fisiologia , Cães , Emoções , Feminino , Frequência Cardíaca/fisiologia , Humanos , Masculino , Motivação , Saliva
12.
Arch Oral Biol ; 111: 104648, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31927405

RESUMO

OBJECTIVE: Caries and periodontitis are uncommon in free ranging great apes but a major oral disease in humans. The aim was to analyze abundance and diversity of oral bacteria of western humans and their closest relatives, to examine if zoo apes feeding on diet other than in their natural habitat show caries and periodontitis associated salivary bacteria and comparable susceptibility for oral civilization diseases as humans. DESIGN: Bacterial composition of human and great ape saliva samples were compared by analyzing the V3 region of the bacteria 16S rRNA gene by Next Generation Sequencing with Ion Torrent. RESULTS: Results show species-specific differences in the salivary bacteria phyla and genera composition among all apes. Moreover, salivary bacterial composition within non-human apes showed higher intra-individual differences than within humans. Human saliva exhibited lowest bacteria diversity. Different behavioral patterns including (oral) hygiene standards of humans and non-human apes might cause differences. All species differed in diversity and abundance of caries associated bacteria genera. Human saliva revealed higher abundance of caries and periodontitis relevant bacteria in contrast to other great apes, which might be supported by higher consume of refined cariogenic food items, possibly raising their risk for oral disease susceptibility. CONCLUSIONS: The study offers first clues on caries and periodontitis relevant bacteria of captive great ape species in comparison to humans. Higher susceptibility to oral diseases for humans than for their closest relatives, leads to the question, if the oral microbiome changed during evolution and how it is influenced by the human life style.


Assuntos
Cárie Dentária , Periodontite , Animais , Bactérias , Hominidae , Humanos , RNA Ribossômico 16S , Saliva
13.
Arch Oral Biol ; 111: 104651, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31931277

RESUMO

OBJECTIVES: The preservation of enamel mineral is influenced by the supersaturation of salivary secretions with respect to calcium phosphate salts. The aim was to measure the chemical environmental state of phosphate ions in a subject's parotid saliva, and to correlate this with their dental caries score, by means of 31P-Nuclear Magnetic Resonance Spectroscopy (31P-NMR) and the International Caries Detection and Assessment System (ICDAS). DESIGN: Unilateral paraffin wax stimulated parotid saliva samples were collected from 21 healthy adult subjects using a Lashley cup. The flowrate was recorded during collection. Clinical caries scores of each subject were classified using the ICDAS score. The pH was recorded for each saliva sample. 31P-NMR spectra of each saliva sample were obtained to determine the phosphorus chemical environment. All the collected data were analysed by Pearson's correlation. RESULTS: Parotid saliva flow rates were in the range from 0.07 to 0.56 ml/min. The pH varied from 5.9 to 7.6. Each 31P-NMR spectrum showed a single broad line with a chemical shift between 0.07 and 2.38 ppm. At neutral pH the maximum chemical shift was 2.05 ppm, whereas at a lower pH values the phosphorous chemical shift reduced, to 0.34 at pH 5.9. The flowrate and the 31P-NMR chemical shift correlated positively (r = 0.71; p < 0.05). The ICDAS score correlated negatively with the 31P-NMR chemical shift (r = 0.43; p < 0.05). CONCLUSIONS: This parotid saliva 31P-NMR study has shown that different phosphate states exist within saliva, which significantly influence its inorganic chemical behaviour, and therefore its cariostatic activity.


Assuntos
Cárie Dentária , Adulto , Esmalte Dentário , Humanos , Espectroscopia de Ressonância Magnética , Saliva , Salivação
14.
J Clin Pediatr Dent ; 44(1): 28-34, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31995418

RESUMO

Objective: Although mitis-salivarius-bacitracin (MSB) agar is a commonly used selective medium for detecting Streptococcus mutans in clinical studies, non-S. mutans microorganisms are cultivatable on MSB agar. Since few studies have identified non-S. mutans bacteria grown on MSB, this study aimed to identify and differentiate MSB-grown non-S. mutans bacteria from predente infants' oral cavity. Study design: The saliva from 51 predente infants were plated on MSB agars. Bacteria colonies were characterized based on their morphology under direct visualization and light microscopic observation. Colony PCR targeting S. mutans htrA locus and 16S rRNA DNA sequencing were used for further bacteria identification. Results: Overall, 80% of the predente infants had oral bacteria grown on the MSB agar. Nine bacteria were identified, including S. mutans, Staphylococcus epidermidis, Klebsiella quasi-pneumoniae, Klebsiella pneumoniae, Enterobacter kobei, Enterococcus faecalis, Staphylococcus hominis, Streptococcus anginosus and Phytobacter. The most frequently detected bacteria were S. epidermidis (41.5%), followed by E. kobei (24.4%), K. pneumoniae (17.1%) and S. mutans (9.8%.) Conclusions: Multiple non-S. mutans bacteria from infants' oral cavity could grow on MSB agar. Caution should be exercised in counting the colony forming units of S. mutans from oral samples on MSB agar to avoid overestimation by assuming that all colonies on the MSB agar are S. mutans. Using the colony morphological guide we summarized, these non-S. mutans bacteria could be distinguished from S. mutans. Our study provides a key reference to pediatric cariology clinical-epidemiological studies that commonly use MSB to identify/quantify S. mutans in infants and young children.


Assuntos
Bacitracina , Streptococcus mutans , Ágar , Criança , Pré-Escolar , Meios de Cultura , Humanos , Lactente , RNA Ribossômico 16S , Saliva
15.
J Oral Sci ; 62(1): 9-12, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31996532

RESUMO

There are many studies on the homebound and institutionalized elderly; however, few studies focus on centenarians and supercentenarians, i.e., people aged 100 and 110 years, respectively. Due to the demographic changes, the population of centenarians is set to increase more than that of other age groups. Therefore, this article aims to review the available literature regarding how oral health might display in this age group and highlight aspects necessitating further research. Oral health, oral health-related quality of life, saliva, and the oral microbiome were emphasized in this study. Most papers relevant to the research questions were excluded because the mean age of participants was <100 years. Only two papers were found on centenarians' oral health status and saliva and no studies were found focusing on the oral health-related quality of life or oral microbiome. The reviewed studies demonstrate that centenarians presented with good oral mucosal conditions, dental conditions, and general health. The present literature is insufficient to come to a definite conclusion regarding how aging affects the oral health of centenarians and supercentenarians. The limited available research indicates that centenarians display better oral health than other individuals in their respective birth cohorts.


Assuntos
Saúde Bucal , Qualidade de Vida , Idoso , Idoso de 80 Anos ou mais , Humanos , Saliva
16.
Anticancer Res ; 40(1): 191-199, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31892567

RESUMO

AIM: To evaluate the feasibility of conducting studies of saliva circulating tumor DNA (ctDNA) as a biomarker of metastasis or recurrence in our orthotopic head and neck cancer (HNC) mouse model. MATERIALS AND METHODS: A mouse model of recurrence or metastasis after tongue cancer resection was developed. Blood and saliva were collected at baseline and at the establishment of recurrence or metastasis. Real-time polymerase chain reaction was performed to quantify human long interspersed element (hLINE) in samples to assess the amount of ctDNA. RESULTS: In our model, salivary hLINE increased as the cancer developed and decreased after surgery. Plasma hLINE was significantly elevated in mice with metastasis. The presence of tongue cancer recurrence in mice was more correlated with hLINE concentration in saliva than in plasma. CONCLUSION: In our orthotopic model, salivary ctDNA better reflected tumor development and recurrence than did plasma ctDNA.


Assuntos
Biomarcadores Tumorais , DNA Tumoral Circulante , Neoplasias de Cabeça e Pescoço/metabolismo , Saliva/metabolismo , Animais , Apoptose/genética , Linhagem Celular Tumoral , Modelos Animais de Doenças , Feminino , Neoplasias de Cabeça e Pescoço/patologia , Xenoenxertos , Humanos , Elementos Nucleotídeos Longos e Dispersos , Camundongos , Recidiva
17.
Med Oral Patol Oral Cir Bucal ; 25(2): e195-e204, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31967985

RESUMO

BACKGROUND: The main objective of this study was to estimate the prevalence of human papillomavirus-DNA (HPV-DNA) in the saliva of sexually active women with HPV-related cervical intraepithelial neoplasias (CIN) and compare the findings with a healthy control group. The secondary objectives were: 1) to determine the concordance between genital and oral HPV types in sexually active women with HPV-related CIN; 2) to analyze whether sexual habits influence the presence of HPV-related CIN; 3) to determine whether sexual habits influence the presence of oral HPV. MATERIAL AND METHODS: Saliva samples were collected from 100 sexually active women, 50 with HPV-related CIN and 50 healthy subjects presenting normal cytology. PCR assay was used to detect HPV-DNA. RESULTS: The prevalence of oral HPV infection in saliva samples was 14% in women with HPV-related CIN, while in the healthy group it was 12%, without statistically significant difference (p=0.766). As for the concordance between genital and oral HPV types in women with HPV-related CIN, concordance was only observed for HPV-16, whereby among 22 women with genital HPV-16, only one (4.54%) also presented oral HPV-16. Regarding the possible influence of sexual habits on the presence of cervical pathology and presence of oral HPV, it was found that marital status, age at first intercourse, number of lifetime sexual partners, and condom use are related with the presence of cervical pathology (p<0.001; p=0.017; p=0.002; and p<0.001, respectively); condom use was also found to be related to the presence of oral HPV (p<0.001). CONCLUSIONS: The prevalence of HPV-DNA in the saliva of sexually active women with HPV-related CIN is similar to healthy women. The concordance between genital and oral HPV types is low. Both the presence of cervical pathology and the presence of oral HPV are related to sexual habits. Wider sample size is required to confirm this results.


Assuntos
Neoplasia Intraepitelial Cervical , Papillomaviridae , Infecções por Papillomavirus , Neoplasias do Colo do Útero , Feminino , Humanos , Prevalência , Fatores de Risco , Saliva
18.
Eur J Endocrinol ; 182(2): 207-217, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31804965

RESUMO

Objective: Monitoring of patients with Cushing's disease on cortisol-lowering drugs is usually performed with urinary free cortisol (UFC). Late-night salivary cortisol (LNSC) has an established role in screening for hypercortisolism and can help to detect the loss of cortisol circadian rhythm. Less evidence exists regarding the usefulness of LNSC in monitoring pharmacological response in Cushing's disease. Design: Exploratory analysis evaluating LNSC during a Phase III study of long-acting pasireotide in Cushing's disease (clinicaltrials.gov: NCT01374906). Methods: Mean LNSC (mLNSC) was calculated from two samples, collected on the same days as the first two of three 24-h urine samples (used to calculate mean UFC [mUFC]). Clinical signs of hypercortisolism were evaluated over time. Results: At baseline, 137 patients had evaluable mLNSC measurements; 91.2% had mLNSC exceeding the upper limit of normal (ULN; 3.2 nmol/L). Of patients with evaluable assessments at month 12 (n = 92), 17.4% had both mLNSC ≤ULN and mUFC ≤ULN; 22.8% had mLNSC ≤ULN, and 45.7% had mUFC ≤ULN. There was high variability in LNSC (intra-patient coefficient of variation (CV): 49.4%) and UFC (intra-patient CV: 39.2%). mLNSC levels decreased over 12 months of treatment and paralleled changes in mUFC. Moderate correlation was seen between mLNSC and mUFC (Spearman's correlation: ρ = 0.50 [all time points pooled]). Greater improvements in systolic/diastolic blood pressure and weight were seen in patients with both mLNSC ≤ULN and mUFC ≤ULN. Conclusion: mUFC and mLNSC are complementary measurements for monitoring treatment response in Cushing's disease, with better clinical outcomes seen for patients in whom both mUFC and mLNSC are controlled.


Assuntos
Ritmo Circadiano , Hidrocortisona/metabolismo , Hipersecreção Hipofisária de ACTH/metabolismo , Adenoma Hipofisário Secretor de ACT/complicações , Adenoma Hipofisário Secretor de ACT/cirurgia , Adulto , Feminino , Hormônios/uso terapêutico , Humanos , Hidrocortisona/urina , Masculino , Pessoa de Meia-Idade , Hipersecreção Hipofisária de ACTH/tratamento farmacológico , Hipersecreção Hipofisária de ACTH/etiologia , Hipersecreção Hipofisária de ACTH/urina , Neoplasias Hipofisárias/complicações , Neoplasias Hipofisárias/cirurgia , Saliva/química , Somatostatina/análogos & derivados , Somatostatina/uso terapêutico , Resultado do Tratamento
19.
J Oral Pathol Med ; 49(1): 63-71, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31529714

RESUMO

BACKGROUND: Acinar progenitor cells within salivary glands have decreased regenerative capacity and exhibit shorter telomeres in primary Sjögren's syndrome (pSS) patients. We investigated whether DNA of saliva, PBMCs, and labial salivary gland (LSG) biopsy tissue have shorter telomeres in pSS compared to controls. mRNA expression of genes associated with pSS pathogenesis (ETS1, LEF1, and MMP9), telomere DNA damage response (ATM), senescence (CDKN2A), telomerase inhibition (IFN-y, TGFß1), and the shelterin complex (TPP1, POT1) were assessed in LSG tissue by qRT-PCR to examine potential defects in telomere maintenance. METHODS: Relative telomere length in DNA of saliva, PBMCs, and LSGs from non-pSS sicca and pSS patients was measured using qPCR. Saliva DNA telomere length was further compared to healthy controls. Expression of genes affecting telomere maintenance was analyzed in LSGs using qRT-PCR. RESULTS: Primary Sjögren's syndrome patients have shorter telomeres in saliva DNA (n = 21) than healthy controls (n = 27) (P = .0035). ATM mRNA expression was higher in pSS LSG tissue (n = 16) vs non-pSS sicca patients (n = 13) (P = .0283) and strongly correlated with LEF1, TPP1, and POT1 (P < .01, r > 0.6). CONCLUSIONS: Patients with pSS exhibited significant telomere erosion in saliva DNA. Overexpression of ATM in LSGs could represent a compensatory response to telomere shortening. The role of LEF1 in telomere erosion remains to be elucidated.


Assuntos
Síndrome de Sjogren , Humanos , Saliva , Glândulas Salivares , Glândulas Salivares Menores , Telômero
20.
Chemosphere ; 241: 124899, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31586830

RESUMO

Recent studies have found elevated dioxin levels inside some former US military air bases in Vietnam, known as hotspots. The aim of the present study was to evaluate the association of dioxin exposure and steroid hormone in preschool children in Vietnam. In 2010, 2011, 52 primiparae mother-infant pairs in the hotspot and 52 pairs in a non-exposure region were enrolled. For the final analysis, 26 vs 26 pairs were selected, who participated at all three surveys. Univariable and multivariable linear regressions were used to evaluate associations between hormone and dioxin congeners. Geometric mean total TEQ of PCDD/DFs in the hotspot were significantly higher than in the non-exposure region, 8.7 and 3.4 pg TEQ/g lipid, respectively. In the hotspot, salivary dehydroepiandrosterone (DHEA) was significantly higher in 1-year-old children (Boys = 123 pg/mL, Girls = 120 pg/mL) than in the non-exposure region (Boys = 28 pg/mL, Girls = 27 pg/mL). In contrast, DHEA was significantly lower in 5-year-old children (Boys = 70 pg/mL, Girls = 106 pg/mL) in the hotspot than in the non-exposure region (Boys = 496 pg/mL, Girls = 654 pg/mL). Salivary testosterone was significantly lower in the hotspot (Boys = 1.9 pg/mL, Girls = 1.9 pg/m; Boys = 1.0 pg/mL, Girls = 1.1 pg/mL, respectively) than in the non-exposure region (Boys = 3.7 pg/mL, Girls = 3.8 pg/m; Boys = 5.7 pg/mL, Girls = 7.0 pg/mL, respectively) at 3 years and 5 years of age. Higher levels of highly chlorinated dioxins in breast milk were associated with higher DHEA in 1-year-old and lower DHEA and testosterone levels in 5-year-old children. Our findings indicated that dioxins were associated with changes of DHEA and testosterone levels in preschool Vietnamese children.


Assuntos
Dioxinas/análise , Poluentes Ambientais/análise , Leite Humano/química , Esteroides/análise , Pré-Escolar , Estudos de Coortes , Desidroepiandrosterona/análise , Feminino , Seguimentos , Humanos , Lactente , Masculino , Saliva/química , Testosterona/análise , Vietnã
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA