Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 11.335
Filtrar
1.
Adv Gerontol ; 32(3): 364-369, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31512422

RESUMO

Among the diseases of the cardiovascular system in elderly people, ischemic heart disease and myocardial infarction (MI) occupy the first place in the structure of mortality. One of the main causes of disability and death from MI is late diagnosis. In this regard, the search for new, highly informative and non-invasive methods for diagnosing MI is an important task of molecular gerontology. An enzyme immunoassay showed that the concentration of TNF-α, IL-8 cytokines and p16 aging marker in saliva in elderly people without cardiovascular pathologies (CP) increases in 2,1-4,8 times as compared with middle-aged people. At the same time, in elderly people without CP the concentration in the saliva of the hormone irisin (FNDC5) decreases by 1,8 times as compared with middle-aged people. In middle-aged patients with MI the concentration of IL-8, TNF-α, MMP8, MMP9 in saliva increases 4,3-15,3 times, and FNDC5 decreases 1,8 times compared with those parameters without CP in this age group. In elderly people with MI the concentration of IL-8, TNF-α, MMP8 and MMP9 in saliva increases 4,3-7,1 times as compared with elderly people without CP. Thus, the study of the concentration of signaling molecules IL-8, TNF-α, MMP8, MMP9 in saliva can be used as a non-invasive method for diagnosing MI in people of middle and elderly age. To assess the rate of aging of the organism in middle-aged and elderly people without CP, a study of the concentration of p16 and FNDC5 molecules in saliva is recommended.


Assuntos
Envelhecimento , Infarto do Miocárdio , Saliva , Idoso , Envelhecimento/metabolismo , Biomarcadores/metabolismo , Citocinas/metabolismo , Humanos , Pessoa de Meia-Idade , Infarto do Miocárdio/diagnóstico , Saliva/química , Fator de Necrose Tumoral alfa/metabolismo
2.
Adv Gerontol ; 32(3): 477-482, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31512438

RESUMO

In 275 (69 men and 206 women) aged 25 to 86 years, including 74 elderly and old people suffering from type 2 diabetes mellitus, chronic nonspecific inflammatory bowel diseases, alcoholism, chronic kidney disease with the use of various laboratory methods, age-specific features of physical and chemical properties and microcrystallization of oral fluid were studied. It is shown that age-related changes in the composition and properties of mixed saliva and the peculiarities of its microcrystallization are due not only to the aging process, but also to concomitant chronic diseases. As you age, with a decrease in salivation in the oral fluid is determined by increasing the total protein content, reducing the Ca/P ratio, as well as increasing its viscosity. Age dynamics of microcrystallization of oral fluid in healthy adults was revealed. At young and middle age, types I and IIA of mixed saliva microcrystallization are more common at the length of the main axes of dendritic crystals of oral fluid equal, respectively, to 802,6±79,3 µm and 792,2±98,7 µm. In persons of older age groups without clear clinical manifestations of chronic diseases, IIB and IIC microcrystallization of oral fluid are more common with a decrease in the length of the main axes of dendritic crystals, respectively, to 618±77,3 µm and 581,4±69,9 µm. In the presence of clinically expressed psychosomatic pathology in elderly and senile people, the second and third types of microcrystallization of oral fluid are more common with a significant reduction in the length of the main axes of dendritic crystals of oral fluid to 413,2±94,6 µm.


Assuntos
Saliva , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Cristalização , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Saliva/química
3.
Braz Oral Res ; 33: e043, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31508727

RESUMO

Proteomic techniques have become popular in medicine and dentistry because of their widespread use in analyzing bodily fluids such as blood, saliva, urine, and gingival crevicular fluids as well as hard tissues such as enamel, dentine, and cementum. This review is a guide to proteomic techniques in general dentistry, summarizing techniques and their clinical application in understanding and diagnosing diseases and their use in identifying biomarkers of various diseases.


Assuntos
Proteoma , Proteômica/métodos , Saliva/química , Proteínas e Peptídeos Salivares/química , Biomarcadores/química , Eletroforese em Gel de Poliacrilamida/métodos , Humanos , Espectrometria de Massas/métodos , Neoplasias Bucais/diagnóstico , Síndrome de Sjogren/diagnóstico
4.
J Appl Oral Sci ; 27: e20180671, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31508795

RESUMO

OBJECTIVE: To monitor early periodontal disease progression and to investigate clinical and molecular profile of inflamed sites by means of crevicular fluid and gingival biopsy analysis. METHODOLOGY: Eighty-one samples of twenty-seven periodontitis subjects and periodontally healthy individuals were collected for the study. Measurements of clinical parameters were recorded at day -15, baseline and 2 months after basic periodontal treatment aiming at monitoring early variations ofthe clinical attachment level. Saliva, crevicular fluid and gingival biopsies were harvested from clinically inflamed and non-inflamed sites from periodontal patients and from control sites of healthy patients for the assessment of IL-10, MMP-8, VEGF, RANKL, OPG and TGF-ß1 protein and gene expression levels. RESULTS: Baseline IL-10 protein levels from inflamed sites were higher in comparison to both non-inflamed and control sites (p<0.05). Higher expression of mRNA for IL-10, RANK-L, OPG, e TGF-ß1 were also observed in inflamed sites at day -15 prior treatment (p<0.05). After the periodontal treatment and the resolution of inflammation, seventeen percent of evaluated sites still showed clinically detectable attachment loss without significant differences in the molecular profile. CONCLUSIONS: Clinical attachment loss is a negative event that may occur even after successful basic periodontal therapy, but it is small and limited to a small percentage of sites. Elevated inflammation markers of inflamed sites from disease patients reduced to the mean levels of those observed in healthy subjects after successful basic periodontal therapy. Significantly elevated both gene and protein levels of IL-10 in inflamed sites prior treatment confirms its modulatory role in the disease status.


Assuntos
Perda da Inserção Periodontal/patologia , Periodontite/patologia , Adulto , Biomarcadores/análise , Biópsia , Estudos de Casos e Controles , Citocinas/análise , Feminino , Gengiva/patologia , Líquido do Sulco Gengival/química , Humanos , Masculino , Metaloproteinase 8 da Matriz/análise , Pessoa de Meia-Idade , Osteoprotegerina/análise , Periodontite/terapia , Reação em Cadeia da Polimerase em Tempo Real , Saliva/química , Estatísticas não Paramétricas , Fatores de Tempo , Fator A de Crescimento do Endotélio Vascular/análise
5.
Vet Parasitol ; 272: 44-52, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31395204

RESUMO

In the present study, a quantitative proteomic approach to study changes in saliva proteins associated with canine leishmaniosis (CanL) was performed. For this, canine salivary proteins were analysed and compared between dogs before (T0) and after (T1) experimental infection with Leishmania infantum by high-throughput label-based quantitative LC-MS/MS proteomic approach and bioinformatic analysis of the in silico inferred interactome protein network was created from the initial list of differential proteins. More than 2000 proteins were identified, and of the 90 differentially expressed proteins between T0 and T1, 12 were down-regulated with log2 fold change lower than -0.5849, and 19 were up-regulated with log2 fold change greater than 0.5849. This study provides evidence of changes in salivary proteome that can occur in canine leishmaniosis and revealed biological pathways in saliva modulated in canine leishmaniosis with potential for further targeted research.


Assuntos
Doenças do Cão/fisiopatologia , Leishmaniose/veterinária , Saliva , Proteínas e Peptídeos Salivares/genética , Proteínas e Peptídeos Salivares/metabolismo , Animais , Cromatografia Líquida , Simulação por Computador , Cães , Regulação da Expressão Gênica , Leishmaniose/fisiopatologia , Proteoma/genética , Proteoma/metabolismo , Proteômica , Saliva/química , Saliva/metabolismo , Espectrometria de Massas em Tandem
6.
Stud Health Technol Inform ; 264: 1724-1725, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31438312

RESUMO

This paper explains a methodology to improve patient safety through early detecting of HF complications and analyzing HF biomarkers in saliva obtained using a biosensor developed in HEARTEN project. A pilot study was performed in two hospitals in Spain and Italy respectively. A direct correlation was identified between TNF-alpha levels in saliva and weight. The weight gain in HF patients could predict a HF decompensation, consequently TNF-alpha could be a new biomarker of these decompensations.


Assuntos
Insuficiência Cardíaca , Saliva , Fator de Necrose Tumoral alfa/análise , Biomarcadores , Técnicas Biossensoriais , Insuficiência Cardíaca/diagnóstico , Humanos , Itália , Projetos Piloto , Saliva/química , Espanha
7.
J Agric Food Chem ; 67(34): 9579-9590, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31381329

RESUMO

The first contact of tannins with the human body occurs in the mouth, where some of these tannins are known to interact with salivary proteins, in particular with proline-rich proteins (PRPs). These interactions are important at a sensory level, especially for astringency development, but could also affect the biological activities of the tannins. This study gathers information on the relative affinity of the interaction, complex stoichiometry, and tannin molecular epitopes of binding for the interactions between the families of PRPs (bPRPs, gPRPs, and aPRPs) and three representative ellagitannins (castalagin, vescalagin, and punicalagin). These interactions were studied by saturation-tranfer difference NMR and microcalorimetry. The effect of the PRP-ellagitannin interaction on their antioxidant ability was also assessed by ferric reduction antioxidant power (FRAP) assays. The results support a significant interaction between the studied tannins and PRPs with binding affinities in the micromolar range. Punicalagin was always the ellagitannin with higher affinity. aPRPs were the salivary PRPs with higher affinity. Moreover, it was observed that when ellagitannins are present in low concentrations (5-50 µM), as occurs in food, the antioxidant ability of these tannins when complexed with salivary PRPs could be significantly impaired.


Assuntos
Taninos Hidrolisáveis/química , Proteínas Salivares Ricas em Prolina/química , Antioxidantes/química , Antioxidantes/metabolismo , Adstringentes/química , Adstringentes/metabolismo , Humanos , Taninos Hidrolisáveis/metabolismo , Cinética , Ligação Proteica , Saliva/química , Saliva/metabolismo , Proteínas Salivares Ricas em Prolina/metabolismo , Paladar
8.
Community Dent Health ; 36(3): 187-189, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31436924

RESUMO

OBJECTIVE: To evaluate the feasibility of collecting and analyzing saliva samples from dental practices and patients' homes for biochemical verification of tobacco use status. BASIC RESEARCH DESIGN: Sub-study within single-arm, multi-center, longitudinal clinical study. CLINICAL SETTING: Dental practices in the South Central region of the United States National Dental Practice-Based Research Network and patients' homes. PARTICIPANTS: Fifty-five patients recruited from 30 dental practices. INTERVENTIONS: Participants in the sub-study were instructed on saliva collection for cotinine analysis in dental practices where they enrolled in the primary study. Saliva was collected at the practices and then from patients' homes. MAIN OUTCOME MEASURES: Feasibility for dental practice collection was define as 80% of enrolled participants having analyzable samples. For patients' home collection, feasibility was defined as 70%. RESULTS: Forty-seven samples (i.e., 86% of those enrolled) collected in dental practices were analyzable. Twenty-one samples (i.e. 38% of those enrolled) collected in patients' homes were analyzable. CONCLUSIONS: Collecting saliva samples for cotinine analysis from dental practices, but not from patients' homes, was feasible. Dental practices may provide an advantageous setting for biochemically verifying tobacco use status as part of clinical trials for tobacco cessation.


Assuntos
Cotinina , Abandono do Hábito de Fumar , Uso de Tabaco , Cotinina/análise , Assistência Odontológica , Estudos de Viabilidade , Feminino , Humanos , Masculino , Saliva/química
9.
Am J Dent ; 32(4): 183-186, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31436938

RESUMO

PURPOSE: To investigate the association between periodontitis and levels of biochemical markers as well as enzyme activity. METHODS: Unstimulated whole saliva samples were obtained from 30 patients with periodontitis. Biochemical factors including the levels of malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), uric acid (UA), and lactoferrin, as well as ß-hexosaminidase (ß-HEX) activity were measured. RESULTS: The levels of a salivary oxidant such as MDA and NO were statistically significantly higher in periodontitis patients than to that of healthy individuals. Similarly, the results indicated elevated levels of lactoferrin and ß-HEX activity in saliva of the periodontitis group, which was statistically significant when compared to the controls. While the levels of an enzymatic antioxidant such as SOD were higher in the periodontitis patients than in the control subjects, uric acid levels were statistically significantly lower in the saliva of the periodontitis patients than in the healthy controls. CLINICAL SIGNIFICANCE: Except for uric acid, as a non-enzymatic antioxidant, the levels of salivary oxidative stress generally increase in the saliva of periodontitis patients. Since altered levels of salivary biomarkers such as oxidative stress and antioxidant substances might contribute in systemic and local complications in the patients, these informative biomarkers can be used as a promising factor for the early diagnosis of the disease.


Assuntos
Biomarcadores , Periodontite Crônica , Estresse Oxidativo , Periodontite , Antioxidantes/análise , Biomarcadores/análise , Periodontite Crônica/diagnóstico , Humanos , Malondialdeído/análise , Periodontite/diagnóstico , Saliva/química
10.
Klin Lab Diagn ; 64(7): 405-408, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31408591

RESUMO

The purpose of the investigation was to study the efficiency of measuring markers of oxidative stress and muscle damage in the oral fluid in highly skilled sportsmen under physical exercise for the assessment of their functional state. 70 highly qualified athletes at the age of 16-20 years specializing in the cyclic kinds of sports (track and field, swimming) took part in the investigation. Sportsmen performed the control test which consisted of the series of 3×100 m distances by a flat race with an active 45 s rest between them for the track and field athletes, and 4×50 m by the main swimming style with an active rest between the distances also for 45 s for the swimmers. Activity of creatine kinase, content of lipid peroxidation products in the blood and oral fluid were measured standard biochemical methods. The performance of the functional tests induces the excessive accumulation of toxic products of lipoperoxidation and increases activity of creatine kinase in the oral fluid of highly qualified athletes. Correlation analysis shows, that the intensity of free radical and peroxide processes in athletes can be evaluated by means of the method of measuring the content of lipid peroxidation products in the oral fluid.


Assuntos
Atletas , Exercício/fisiologia , Peroxidação de Lipídeos , Saliva/química , Creatina Quinase/análise , Humanos , Peróxidos Lipídicos/análise
11.
Klin Lab Diagn ; 64(7): 413-416, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31408593

RESUMO

The selective properties of a solution of oligonucleotide specific to IL-6 on the concentration of IL-6 in mixed saliva of patients with oral inflammatory processes were studied using SDS-PAGE by electrophoresis and enzyme immunoassay. The application of these methods showed that in the mixed saliva of patients after rinsing with a solution of an oligonucleotide specific for IL-6, the amount of IL-6 decreases. The ELISA Kit and 20% SDS-PAGE showed the highest sensitivity to determine the concentration of IL-6 in saliva, which should be considered in clinical laboratory practice.


Assuntos
Interleucina-6/análise , Antissépticos Bucais/administração & dosagem , Oligonucleotídeos/administração & dosagem , Saliva/química , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Humanos , Boca , Sensibilidade e Especificidade
12.
Anal Bioanal Chem ; 411(23): 6141-6153, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31292703

RESUMO

The present work describes the optimization and validation of an analytical method for the determination of six antipsychotic drugs (chlorpromazine, levomepromazine, cyamemazine, clozapine, haloperidol, and quetiapine) in oral fluid samples after solvent extraction from dried saliva spots, by gas chromatography coupled to tandem mass spectrometry. The method was fully validated, and the included parameters were selectivity, linearity, limits of quantification, precision and accuracy, stability, and recovery. The method was linear for all compounds from 10 to 400 ng/mL, except for haloperidol (5-100 ng/mL), presenting coefficients of determination higher than 0.99. Inter- and intra-day precision and accuracy were in conformity with the criteria usually seen in bioanalytical method validation; i.e., coefficients of variation were lower than 15% and an accuracy of 15% or better for all studied drugs. The recoveries obtained with this miniaturized technique ranged from 63 to 97%. The herein described method is the first to be reported using the dried saliva spots approach for the analysis of these antypshychotic drugs, proving great sensitivity apart from its simple and fast procedure. The method was considered a good alternative to the conventional techniques to be applied in clinical and toxicological analyses, even more taking into account the extremely low sample volume used (50 µL). Graphical abstract.


Assuntos
Antipsicóticos/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Saliva/química , Espectrometria de Massas em Tandem/métodos , Dessecação , Desenho de Equipamento , Cromatografia Gasosa-Espectrometria de Massas/instrumentação , Humanos , Limite de Detecção , Espectrometria de Massas em Tandem/instrumentação
13.
Ned Tijdschr Tandheelkd ; 126(7-8): 385-388, 2019 Jul.
Artigo em Holandês | MEDLINE | ID: mdl-31309937

RESUMO

During periodontal inflammation, a multitude of substances both from the host and of microbiological origin are released into the gingival crevicular fluid (GCV) and saliva. These substances, such as proteins and peptides, can therefore be regarded as biomarkers of the inflammatory process. With the help of sensitive and advanced laboratory technologies, the role of these biomarkers has been identified. However, the high costs, complexity and difficulty of interpretation of the results often hinder the introduction of biomarkers for diagnostic purposes in the dental practice. Certain salivary enzymes, proteases, can function as biomarkers and have interesting properties for producing rapid chairside diagnoses, because the presence of a protease or proteolytic activity can be demonstrated in a simple and rapid biochemical way, for example by colour signalling. Since other processes in the oral cavity influence the results of the test, such tests are especially useful as one element in a broader oral diagnostic investigation.


Assuntos
Líquido do Sulco Gengival/química , Peptídeo Hidrolases/análise , Periodontite , Saliva/química , Biomarcadores , Humanos , Periodontite/diagnóstico
14.
Stomatologiia (Mosk) ; 98(3): 31-34, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31322591

RESUMO

The aim of the study was to elucidate of changes in the level of lipid peroxidation products in saliva for the development of a method for the early diagnosis of complications in dental implantation. The study included 53 patients with complications of implant treatment, 31 patients with a safe outcome of dental implantation, and 13 clinically healthy individuals. In the saliva obtained at the stage of preparation for dental implantation, the content of products of lipid peroxidation was determined. Re-saliva was taken four months after dental implantation or in the case of peri-implantitis verification. It is established that the development of inflammatory complications of dental implantation is accompanied by the development of oxidative stress, which leads to local accumulation of lipoperoxidation products. An increase in the salivary level of products of lipid peroxidation during dental implantation is observed before the formation of clinically significant changes in the tissues of the implantation canal and is associated with a high risk of development of inflammatory complications of dental implantation. The most informative and prognostically unfavorable is the increase in the salivar level of the Schiff bases.


Assuntos
Implantes Dentários , Peroxidação de Lipídeos , Peri-Implantite , Saliva , Implantação Dentária , Implantes Dentários/efeitos adversos , Humanos , Estresse Oxidativo , Fatores de Risco , Saliva/química
15.
Food Chem ; 298: 125003, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260957

RESUMO

The oral breakdown, sensory properties, and volatile release during mastication of white bread were investigated. The results of correlation analysis for white bread's physical properties and it's oral physiological parameters during chewing have elucidated that bread's physical properties determined the oral processing behavior. During chewing of white bread, 15 dominant ions with regularly changing patterns were monitored by proton transfer reaction-mass spectrometry (PTR-MS). These dominant ions derived from 32 volatile compounds were further confirmed by pure standards. Partial least squares regression (PLSR) analysis was used to explore the positive correlations between the sensory analysis and the dominant aroma compounds. Results have shown that 9 aroma compounds were predicted as the potent odorants contributing to the changes in aroma profiles. Finally, 3-hydroxy-2-butanone, 2-methyl-1-propanol, and heptanoic acid were confirmed as the key aroma compounds contributing to the changes in aroma profiles of white bread before and after chewing.


Assuntos
Pão , Mastigação , Odorantes/análise , Compostos Orgânicos Voláteis/análise , Acetoína/análise , Adulto , Pão/análise , Butanóis/análise , Feminino , Cromatografia Gasosa-Espectrometria de Massas/métodos , Cromatografia Gasosa-Espectrometria de Massas/estatística & dados numéricos , Ácidos Heptanoicos/análise , Humanos , Análise dos Mínimos Quadrados , Masculino , Espectrometria de Massas/métodos , Espectrometria de Massas/estatística & dados numéricos , Saliva/química , Microextração em Fase Sólida/métodos , Paladar , Triticum/química
16.
Psychiatr Danub ; 31(2): 162-171, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31291220

RESUMO

In this project, we recruited a sample of 150 patients with first episode of psychosis with schizophrenia features (FEP) and 100 healthy controls. We assessed the differences between these two groups, as well as the changes between the acute phase of illness and subsequent remission among patients over 18-month longitudinal follow-up. The assessments were divided into four work packages (WP): WP1- psychopathological status, neurocognitive functioning and emotional recognition; WP2- stress response measured by saliva cortisol during a stress paradigm; cerebral blood perfusion in the resting state (with single photon emission computed tomography (SPECT) and during activation paradigm (with Transcranial Ultrasonography Doppler (TCD); WP3-post mortem analysis in histologically prepared human cortical tissue of post mortem samples of subjects with schizophrenia in the region that synaptic alteration was suggested by WP1 and WP2; WP4- pharmacogenetic analysis (single gene polymorphisms and genome wide association study (GWAS). We expect that the analysis of these data will identify a set of markers that differentiate healthy controls from patients with FEP, and serve as an additional diagnostic tool in the first episode of psychosis, and prediction tool which can be then used to help tailoring individualized treatment options. In this paper, we describe the project protocol including aims and methods and provide a brief description of planned post mortem studies and pharmacogenetic analysis.


Assuntos
Biomarcadores/análise , Transtornos Psicóticos/genética , Feminino , Seguimentos , Estudo de Associação Genômica Ampla , Humanos , Hidrocortisona/análise , Masculino , Farmacogenética , Estudos Prospectivos , Transtornos Psicóticos/complicações , Transtornos Psicóticos/diagnóstico , Transtornos Psicóticos/tratamento farmacológico , Saliva/química , Esquizofrenia/complicações
17.
J Chromatogr A ; 1603: 61-66, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31262514

RESUMO

A simple procedure based on microextraction by packed sorbent (MEPS) has been proposed for the extraction of dichloropane in oral fluids and its determination by ion mobility spectrometry (IMS). Extraction conditions such as type of sorbent (octyl and octadecyl silica), sample pH, number of sample loadings, and elution volume were evaluated to obtain the most appropriate values. Dichloropane was extracted from saliva samples using C8 MEPS, loading with 100 µL sample (adjusted to pH 7) in 4 cycles, washing with 100 µL deionized water, and eluting with 50 µL 2-propanol in 10 cycles. The proposed MEPS procedure has been validated in terms of linearity, accuracy, and precision. A limit of detection of 30 µg L-1 was obtained for dichloropane determination in saliva. The analysis of field and synthetic saliva samples spiked with dichloropane at concentration levels from 250 to 750 µg L-1 provided relative recoveries between 85 and 110%, using the proposed MEPS-IMS procedure. Field oral fluid samples were collected from healthy individuals, blind-spiked from 92 to 278 µg L-1, and analysed by IMS and gas chromatography-mass spectrometry, being the results obtained from both methods statistically comparable. Thus, the proposed MEPS-IMS procedure involves a simple, sensitive, and accurate analysis of dichloropane in saliva.


Assuntos
Cocaína/análogos & derivados , Cromatografia Gasosa-Espectrometria de Massas/métodos , Espectrometria de Mobilidade Iônica/métodos , Saliva/química , Microextração em Fase Sólida/métodos , Cocaína/análise , Humanos , Limite de Detecção
18.
Braz Oral Res ; 33: e033, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31269113

RESUMO

The aim of this study was to evaluate the effect of periodontal treatment on the salivary cytokine levels and clinical parameters of individuals with cerebral palsy (CP) with gingivitis. A non-randomized, clinical trial was conducted in individuals diagnosed with spastic CP. Thirty-eight individuals were enrolled in the study and were categorized according to gingival index scores between 0-1 or 2-3, assigned to groups G2 or G1, respectively. Periodontal treatment comprised oral hygiene instructions, conventional mechanical treatment and 0.12% chlorhexidine applied as an adjunct. Clinical parameters and saliva samples were collected at baseline and at the 15-day follow-up visit. Bleeding on probing and periodontal screening and recording were determined. Non-stimulated saliva samples were obtained, and the salivary flow rate, the osmolality and the levels of cytokines IL-1ß, IL-6, IL-8, IL-10, TNF-α and IL-12p70 were evaluated by a cytometric bead array. The Wilcoxon test, the Mann-Whitney test, Spearman correlation analysis, Poisson regression analysis and an adjusted analysis were performed (α = 0.05). The groups differed significantly in periodontal clinical parameters at baseline and at follow-up. Salivary flow rate and osmolality were similar in both groups at both timepoints. However, TNF-α and IL-1ß levels were higher in G1 than in G2 at baseline. Mechanical treatment resulted in improved clinical parameters for both groups. Furthermore, mechanical treatment resulted in a significant reduction in salivary IL-1ß and IL-8 levels for both groups after treatment. Periodontal treatment performed in individuals with CP and gingivitis reduces the levels of TNF-α, IL-1ß, IL-6 and IL-8.


Assuntos
Biomarcadores/análise , Paralisia Cerebral/complicações , Gengivite/complicações , Gengivite/reabilitação , Periodontite/terapia , Saliva/química , Adolescente , Criança , Citocinas/análise , Profilaxia Dentária/métodos , Feminino , Gengivite/microbiologia , Humanos , Interleucina-10 , Interleucina-1beta/análise , Interleucina-6/análise , Masculino , Concentração Osmolar , Índice Periodontal , Distribuição de Poisson , Saliva/imunologia , Saliva/microbiologia , Fator de Necrose Tumoral alfa/análise
19.
Int J Sports Med ; 40(8): 511-518, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31288290

RESUMO

External counterpulsation (ECP), an electrocardiogram-led sequential compression of lower limbs, has been recently proposed for sports recovery, but research is scant. This study examined the effects of an ECP session upon neuromuscular function (vertical jump and torque/velocity characteristics), biochemical responses (creatine kinase, cortisol, testosterone, alpha-amylase and immunoglobulin-A), and muscle soreness (visual analogue scale) following high-intensity exercise. Twenty-one male team sport athletes (age: 21.6±3.4 yrs; height: 182.7±7.3 cm; body mass: 82.7±9.3 kg) recovered from the fatiguing exercise using either ECP or rest. Data collection was conducted at three separate time points: upon arrival (Pre), post-recovery (Post), and 24 h post-recovery (24hPost). Significant main effects for time were observed for increased torque/velocity slope and for decreased isometric extension peak torque (p<0.001). Significant main effects for time were observed for increased creatine kinase, testosterone, alpha-amylase, and muscle soreness (all p<0.001). Significant interaction effects were observed at post-testing following ECP: Cortisol release and the related decline in testosterone/cortisol ratio were attenuated, and immunoglobulin-A was increased following ECP in comparison to the control (all p<0.05). Following high-intensity exercise, ECP has potentially beneficial effects upon biomarkers of recovery, without affecting the neuromuscular function.


Assuntos
Contrapulsação , Exercício/fisiologia , Fadiga Muscular/fisiologia , Músculo Esquelético/fisiologia , Mialgia/fisiopatologia , Esportes/fisiologia , Biomarcadores/análise , Creatina Quinase/sangue , Humanos , Hidrocortisona/sangue , Imunoglobulina A/análise , Masculino , Saliva/química , Testosterona/sangue , Torque , Adulto Jovem , alfa-Amilases/análise
20.
Microb Pathog ; 135: 103615, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31254603

RESUMO

AIM: The aim of this study was to explore the caries features in hypoplastic Amelogenesis Imperfecta (AI) patients. MATERIALS AND METHODS: A cross-sectional study was performed including 28 patients, 14 with hypoplastic AI and 14 controls for whom Decayed (D), Missed (M) and Filled (F) Teeth (T) were checked for a DMFT index evaluation. Twenty-eight saliva samples, 4 bacterial plaques and 19 teeth were used. Decayed teeth were observed under polarized light and scanning electron microscopy. Salivary pH was measured and saliva bacterial strains were biochemically identified and confirmed by PCR. Bacterial adhesions to tooth surfaces were observed by Scanning Electron Microscopy (SEM) and evaluated by colony enumeration after in vitro culture of Streptococcus mutans and Lactobacillus casei with dental fragments. RESULTS: DMFT indexes were significantly lower in AI patients (mean DMFT = 0.8) compared to controls (mean DMFT = 2.9). Decayed teeth revealed sclerotic, demineralized, invaded and disintegrated zones in dentine. Dental plaques were rich with filamentous bacteria in AI patients. Oral microbiotome of the saliva showed a low rate of Streptococci and a significant high level of Bacillus spp, Enterococcus faecalis and Enterococcus faecium in AI patients. In vitro study showed a significant high adhesion of Lactobacillus casei and a weak adhesion of Streptococcus mutans on AI dental hard tissues. CONCLUSION: Our study showed that hypoplastic AI patients have (i) a low DMFT index, (ii) an alkaline pH of saliva enriched with Bacillus spp, Enterococcus faecalis and Enterococcus faecium and (iii) dental tissues more easily invaded by Lactobacilli than Streptococci. The combination of these bacteria seems to give AI patients protection against dental caries.


Assuntos
Amelogênese Imperfeita/microbiologia , Amelogênese Imperfeita/patologia , Cárie Dentária/microbiologia , Cárie Dentária/patologia , Adolescente , Adulto , Amelogênese Imperfeita/complicações , Bacillus , Bactérias/classificação , Bactérias/isolamento & purificação , Aderência Bacteriana , Biofilmes , Estudos Transversais , Cárie Dentária/complicações , Placa Dentária/microbiologia , Enterococcus , Humanos , Concentração de Íons de Hidrogênio , Lactobacillus casei , Saliva/química , Saliva/microbiologia , Streptococcus mutans , Propriedades de Superfície , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA