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1.
Medicine (Baltimore) ; 98(28): e16373, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31305436

RESUMO

BACKGROUND: Prenatal exposure to depression has been considered as a risk factor for adverse childhood, while it is accompanied by unknown molecular mechanisms. The aim of this study was to identify differentially expressed genes (DEGs) and associated biological processes between cord blood samples from neonates born to mothers who exposed to major depressive disorder (MDD) and healthy mothers. METHODS: The microarray data GSE114852 were downloaded to analyze the mRNA expression profiles of umbilical cord blood with 31 samples exposed to prenatal MDD and 62 samples with healthy mothers. Kyoto Encyclopedia of Genes and Genomes pathway and Gene ontology enrichment analyses were conducted to identify associated biochemical pathways and functional categories of the DEGs. The protein-protein interaction network was constructed and the top 10 hub genes in the network were predicted. RESULTS: The results showed several immunity related processes, such as "phagosome", "Epstein-Barr virus infection", "proteasome", "positive regulation of I-kappaB kinase/NF-kappaB signaling", "interferon-gamma-mediated signaling pathway", and "tumor necrosis factor" presented significant differences between two groups. Most of the hub genes (for example PSMD2, PSMD6, PSMB8, PSMB9) were also associated with immune pathways. CONCLUSION: This bioinformatic analysis demonstrated immune-mediated mechanisms might play a fatal role in abnormalities in fetal gene expression profiles caused by prenatal MDD.


Assuntos
Transtorno Depressivo Maior , Sangue Fetal/metabolismo , Complicações na Gravidez , Biologia Computacional , Transtorno Depressivo Maior/sangue , Transtorno Depressivo Maior/genética , Feminino , Expressão Gênica , Humanos , Gravidez , Complicações na Gravidez/sangue , Complicações na Gravidez/genética , RNA Mensageiro/sangue
2.
Medicine (Baltimore) ; 98(28): e16424, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31305461

RESUMO

Although the changes in DNA methylation are assumed to be due to the association between adverse intrauterine conditions and adult metabolic health, evidence from human studies is rare. Little is known about the changes in DNA methylation present at birth that affect metabolic profiles in childhood. Previous studies have shown that the melanocortin 4 receptor (MC4R) and hepatocyte nuclear factor 4 alpha (HNF4α) genes are associated with obesity and metabolic disorders. Thus, we investigated the associations of the DNA methylation statuses of MC4R and HNF4α in cord blood with metabolic profiles in childhood.We collected data from 90 children 7 to 9 years of age included in the Ewha Birth & Growth Cohort Study in Korea. DNA methylation was analyzed by pyrosequencing. The children were split into 2 groups according to the cutoff triglyceride (TG) levels (<110 and ≥110 mg/dL).The methylation statuses of MC4R and HNF4α at birth were significantly associated with the TG level in childhood (P < .05). It was interesting to note that the methylation statuses of MC4R and HNF4α in cord blood were significantly decreased, whereas childhood body mass index was significantly increased, in children with high TG levels compared with children with low TG levels (P < .05).Our findings show that the methylation statuses of MC4R and HNF4α at birth are associated with metabolic profiles in childhood. These epigenetic modifications occurring in early life may contribute to subsequent metabolic-related disorders. Thus, we suggest that DNA methylation status in cord blood may be predictive of the risk of developing metabolic syndrome.


Assuntos
Metilação de DNA , Fator 4 Nuclear de Hepatócito/genética , Regiões Promotoras Genéticas , Receptor Tipo 4 de Melanocortina/genética , Triglicerídeos/sangue , Índice de Massa Corporal , Criança , Desenvolvimento Infantil , Ilhas de CpG , Epigênese Genética , Feminino , Sangue Fetal/metabolismo , Seguimentos , Fator 4 Nuclear de Hepatócito/metabolismo , Humanos , Estudos Longitudinais , Masculino , Estudos Prospectivos , Receptor Tipo 4 de Melanocortina/metabolismo
3.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(4): 365-370, 2019 Apr 06.
Artigo em Chinês | MEDLINE | ID: mdl-30982269

RESUMO

Objective: To study the pollution status of polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCB), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in cord blood of newborns in an e-waste dismantling area of Guangdong Province. Methods: We recruited 20 eligible mothers and newborns who could meet the inclusion criteria in local hospitals of Guiyu in 2007. The inclusion criteria included directly engaged in dismantling e-waste during pregnancy and within 1 year before pregnancy; living in the e-waste dismantling workshops or the distance between living place and the e-waste dismantling areas was ≤200 m; the father of newborn was directly engaged in electronic waste dismantling for more than 1 year; the frequency of visiting the e-waste dismantling workshop during pregnancy was ≥3 times in a week. Questionnaires and physical examinations were performed on maternal and neonatal, and cord blood was collected from newborns to detect PCDD/Fs, PCB and PBDE. The concentration level of organic pollutants was corrected by the blood lipid content, and the total toxicity equivalent was calculated. The correlation between three compounds was analyzed by Spearman correlation. Results: The mothers of the 20 newborns were (23.45±3.27) years old and lived for more than 5 years. The number of one parent engaged in e-waste dismantling, the mother or father smoking, and parent engaged in e-waste dismantling work were 3, 13, 15 and 19, respectively. The weight of newborns ranged from 2.5 to 3.6 kilogram and the Apgar score was 10 points. No adverse birth outcomes such as preterm birth, malformation or stillbirth were found. The median (maximum, minimum) concentration of PCBs, PCDD/Fs and PBDEs in cord blood were 263.22 (328.29, 244.19), 38.42 (147.49, 12.68), 39.33 (265.11, 14.81) pg/g lipid, respectively. The median (maximum, minimum) of toxic equivalence concentrations of PCDD/Fs and PCB were 3.94 (9.24, 2.69) and 15.95 (26.64, 9.28) pg TEQ/g lipid. PBDE, the proportion of PBDE, PCB and PCDD/Fs in cord blood was 50.41%, 49.25% and 0.34%, respectively. PCBs and PBDEs were positively correlated (r=0.733, P=0.039). Conclusion: The high concentrations of PCDD/Fs, PCB, and PBDE were detected in the e-waste dismantling area. It is recommended that the risk of such substances on the health of local people should be assessed in a timely manner.


Assuntos
Dioxinas/sangue , Resíduo Eletrônico , Exposição Ambiental , Poluentes Ambientais/sangue , Sangue Fetal/metabolismo , Bifenilos Policlorados/sangue , Dibenzodioxinas Policloradas/sangue , Reciclagem , Adulto , Dioxinas/efeitos adversos , Feminino , Humanos , Recém-Nascido , Exposição Materna , Gravidez , Inquéritos e Questionários , Adulto Jovem
4.
Saudi Med J ; 40(4): 353-359, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30957128

RESUMO

OBJECTIVES: To investigate the cord blood levels of adipokine and to assess their association with the fetal insulin resistance and fetal outcomes in newborns of gestational diabetic women (GDM). Methods: This cross-sectional study was performed in 40 GDM women and 40 healthy pregnant women (HPW) in the Department of Obstetrics and Gynecology at Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) hospital in Puducherry, India, during the period from May 2016 to December 2017. Cord blood samples were collected at delivery from GDM and HPW groups. Cord plasma biochemical parameters such as insulin, C-peptide, adiponectin, leptin, resistin, and visfatin concentrations were measured. Leptin/adiponectin ratio (L/A), homeostasis model assessment of insulin resistance (HOMA2-IR), insulin sensitivity (HOMA2-%S) and beta cell function (HOMA2-%B) were calculated. The pregnancy outcomes such as birth weight (BW), Ponderal index and Apgar scores of the baby were measured. Results: The BW and Ponderal index of the baby were found to be significantly higher in GDM newborns compared to HPW newborns. Cord plasma insulin, C-peptide, HOMA2 -IR, visfatin, leptin, and L/A ratio were significantly higher whereas adiponectin level was lower in GDM compared to HPW. A significant positive correlation was observed between L/A ratio and fetal HOMA2-IR. Conclusion: Altered adipokine levels with increased L/A ratio was observed among the new-borns of Indian gestational diabetic mothers. There was an association between increased L/A ratio, insulin resistance and increased Ponderal index among the new-borns.


Assuntos
Adipocinas/sangue , Diabetes Gestacional/sangue , Diabetes Gestacional/fisiopatologia , Sangue Fetal/metabolismo , Resistência à Insulina , Resultado da Gravidez , Índice de Apgar , Biomarcadores/sangue , Peso ao Nascer , Peptídeo C/sangue , Estudos Transversais , Diabetes Gestacional/diagnóstico , Feminino , Humanos , Índia , Recém-Nascido , Insulina/sangue , Leptina/sangue , Nicotinamida Fosforribosiltransferase/sangue , Gravidez , Resistina/sangue
5.
Transfus Apher Sci ; 58(2): 169-173, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30890311

RESUMO

Bone marrow transplantation is a treatment used for hematologic and non-hematologic disorders. A theory suggests that proliferation of cells in non-body condition helps to increase the efficiency of bone marrow transplant. There are different ways for proliferation of stem cells, in which, most studies have focused on stem cell culture in body-like conditions. The use of amniotic fluid as a rich resource of growth factors is developing in repair of tissues cornea. With regards to this condition, we discuss about the influence of amniotic fluid in proliferation and implantation of blood stem cells. The aim of this study was investigation of human amnion fluid (HAF) in support of growth and proliferation of umbilical cord in order to transplant and long period erythropoiesis. First, separating of CD-34+ stem cells by MACS was performed and check in 5% and 8% concentration of amniotic fluid (AF) in comprise with FBS10% in culture environment. After 7, 14 days cell count, and checking gene expression level of cyclinD1, BCL2, CXCR4, SDF1 by real-time PCR. The result show that BCL2, CXCR4 and cyclinD1 gene expression level were increased in cells that are growth in 5% AF with 5% FBS than other groups. After statistical analysis, proliferation of umbilical cord blood stem cells in 5% AF with 5% FBS was more than 8% AF with 2% FBS and 10% FBS. Therefore, HAF can play an effective role in increasing hematopoietic stem cells in cell culture before bone marrow transplant.


Assuntos
Líquido Amniótico/metabolismo , Transplante de Medula Óssea/métodos , Sangue Fetal/metabolismo , Células-Tronco Hematopoéticas/metabolismo , Sangue Fetal/citologia , Células-Tronco Hematopoéticas/citologia , Humanos
6.
Clin Biochem ; 67: 40-47, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30831089

RESUMO

BACKGROUND: Reference intervals for arterial and venous umbilical cord blood gas (UCBG) parameters are scarce, are mainly focused on pH, pO2, pCO2 and base deficit, and are usually assessed using parametric tests, despite a generally skewed data distribution. Here, the purpose is to determine reference percentiles for nine parameters of concomitant arterial and venous UCBG (CAV-UCBG) from neonates at birth, using nonparametric tests. METHODS: Results of CAV-UCBG, assayed over a 4.5-year period, were extracted from a hospital laboratory database for pH, pCO2, pO2, oxygen saturation, concentration of total oxygen, total carbon dioxide, hydrogen carbonate, total haemoglobin, and acid-base excess. Exclusion criteria were: a venous-arterial pH difference <0.02, an arterial-venous pCO2 <0.7 kPa, and a venous pCO2 <2.9 kPa. Nonparametric bivariate kernel density estimations were used for the selection of plots within the 95% percentile surface of the pCO2-to-pH relationship (NBKDE-95P). Outliers from skewed data were removed using an adjusted-Tukey method, and percentiles were calculated according to the CLSI EP28-A3 nonparametric method. RESULTS: Overall, 31% (5033/16164) of CAV-UCBG were discarded using the three exclusion criteria. Then, 6% (670/11131) of CAV-UCBG were excluded from the NBKDE-95P, and 0.1 to 3.5% outliers were subsequently removed. Depending on the parameter, the 2.5th and 97.5th percentiles from the whole group were similar or slightly narrower compared to reference intervals from other studies, while those from female and male neonates did not differ substantially. CONCLUSIONS: Using an indirect nonparametric approach, this study proposes new percentiles for parameters from concomitant arterial and venous umbilical cord blood gases.


Assuntos
Dióxido de Carbono/sangue , Sangue Fetal/metabolismo , Oxigênio/sangue , Artérias Umbilicais , Veias Umbilicais , Feminino , Humanos , Concentração de Íons de Hidrogênio , Masculino
7.
Stem Cell Res ; 35: 101392, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30711803

RESUMO

Here we report the reprogramming of CD34+ cells obtained from UCB of a healthy donor female child belonging to the Indian ethnic population. These CD34+cells were subjected to nucleofection for delivery of episomal vectors expressing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (negative mutation in p53). The iPSC colonies expressed pluripotency markers as detected by PCR, immunofluorescence and flow-cytometry. The removal of plasmid was confirmed by its absence in cells at higher passages. Karyotype analysis revealed a stable genome. The property of in vitro differentiation to tri-lineage was confirmed by expression of markers by immunofluorescence.


Assuntos
Antígenos CD34/metabolismo , Linhagem Celular , Técnicas de Reprogramação Celular , Sangue Fetal , Células-Tronco Pluripotentes Induzidas , Grupo com Ancestrais do Continente Asiático , Reprogramação Celular , Feminino , Sangue Fetal/citologia , Sangue Fetal/metabolismo , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Células-Tronco Pluripotentes Induzidas/metabolismo , Recém-Nascido , Cariótipo
8.
Gene ; 696: 10-20, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30769140

RESUMO

Human umbilical cord (UC) and cord blood (CB) provide attractive sources of mesenchymal stem cells (MSCs) for cell therapy. Both UCMSCs and CBMSCs have been demonstrated to play prominent roles in clinical therapy. However, little is known about their functional differences in clinical application. Our transcriptome analysis uncovered high activity of insulin secretion related signaling pathways for CBMSCs and cell adhesion related signaling pathways for UCMSCs. Expression of a large number of immune related signaling pathways also showed the difference in both cells, implying their distinct immune modulatory functions. As the therapeutic effects of MSCs mainly dependent on the cytokines and growth factors produced by transplanted MSCs, we further compared the cytokine profiles of UCMSCs and CBMSCs using antibody array. By evaluating the expression of 106 cytokines, we found both MSCs abundantly secreted TSP-1, TSG-14, TIMP-1, IL-8, IL-6, CXCL1, GIF and IGFBP3. However, the expression of CCL2 in UCMSCs showed significantly higher than CBMSCs. IGFBP1 and IGFBP2 were secreted by CBMSCs with higher abundance than UCMSCs. Overall, these results suggest that UCMSCs and CBMSCs preserve different functional potentials, which have to be carefully considered before clinical treatment.


Assuntos
Citocinas/metabolismo , Sangue Fetal/citologia , Células-Tronco Mesenquimais/metabolismo , Cordão Umbilical/citologia , Células Cultivadas , Sangue Fetal/metabolismo , Perfilação da Expressão Gênica , Humanos , Análise de Sequência com Séries de Oligonucleotídeos , Cultura Primária de Células , Cordão Umbilical/metabolismo
9.
Pediatr Int ; 61(3): 264-270, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30715770

RESUMO

BACKGROUND: Parturition induces considerable oxidative stress and many inflammatory mediators, such as high mobility group box 1 (HMGB1), are involved from the beginning of the pregnancy to birth. The aim of the present study was to evaluate serum cord blood concentration of diacron-reactive oxygen metabolites (d-ROM), biological antioxidant potential (BAP), and HMGB1 to investigate the perinatal oxidative status of neonates and correlation with mode of delivery, as well as the influence of labor. METHODS: The subjects consisted of 214 neonates delivered at University Hospital "G. Martino", Messina, in a 6 months period. Venous blood samples were collected from the umbilical cord after cord separation. RESULTS: Umbilical cord venous blood HMGB1 was significantly higher in the spontaneous vaginal delivery (SVD) group than in the elective or emergency cesarean section (CS) group (P = 0.018). Regarding labor, there was no significant difference in HMGB1 concentration in umbilical vein blood between the spontaneous and induced labor groups (P = 0.250). Furthermore, d-ROM was significantly different between the SVD group and the elective or emergency CS group (P = 0.044). BAP concentration, however, was not significantly different, not even with regard to mode of labor. CONCLUSION: Oxidation is higher in newborns delivered by SVD than in those delivered by CS, and HMGB1 may be involved in the mechanisms of birth, and responsible for decidual modifications that lead to birth.


Assuntos
Parto Obstétrico/estatística & dados numéricos , Sangue Fetal/metabolismo , Proteína HMGB1/sangue , Estresse Oxidativo/fisiologia , Antioxidantes/metabolismo , Biomarcadores/sangue , Feminino , Humanos , Recém-Nascido , Itália , Trabalho de Parto/metabolismo , Trabalho de Parto/fisiologia , Gravidez , Espécies Reativas de Oxigênio/metabolismo
10.
J Pediatr Endocrinol Metab ; 32(2): 167-172, 2019 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-30730843

RESUMO

Background An adequate maternal vitamin D (vitD) intake is rarely achieved in actual practice. The aim of this study was to assess maternal factors associated with neonatal vitD deficiency. Methods This is a single-institution prospective case-control study. Consecutive single-birth neonates admitted between September 2014 and February 2015 were prospectively enrolled. Serum 25-hydroxyvitamin D (25(OH)D) concentrations were measured by spectrometry. The associations between neonatal vitD deficiency (defined as 25(OH)D <15 ng/mL) and several maternal characteristics, including body mass index (BMI) at delivery, education, health insurance status, birth season, sun exposure time, egg consumption, and vitD supplementation during pregnancy, were examined using multivariable logistic regression and their respective odds ratios (ORs) reported. Results A total of 125 mother-infant dyads were enrolled, with a gestational age of 36.8±2.7 weeks. Fifty-six percent (70/125) of the neonates had vitD deficiency. Maternal factors that were significantly associated with vitD deficiency included winter birth, insufficient sun exposure time, high maternal BMI at delivery, insufficient egg consumption, insufficient vitD supplementation during pregnancy, and disadvantaged health insurance. Disadvantaged insurance status and insufficient vitD supplementation during pregnancy were the two most influential factors of neonatal vitD deficiency, with an OR of 7.5 (95% confidence interval [CI], 2.0-37.6) and 7.0 (95% CI, 2.7-20.7), respectively. Conclusions Neonatal vitD deficiency is very rampant. An individualized vitD supplementation strategy may be developed by taking into consideration pregnant women's socioeconomic status and lifestyles.


Assuntos
Suplementos Nutricionais , Sangue Fetal/metabolismo , Doenças do Recém-Nascido/epidemiologia , Complicações na Gravidez/epidemiologia , Deficiência de Vitamina D/complicações , Deficiência de Vitamina D/epidemiologia , Adulto , Estudos de Casos e Controles , Feminino , Seguimentos , Idade Gestacional , Humanos , Recém-Nascido , Fenômenos Fisiológicos da Nutrição Materna , Estado Nutricional , Gravidez , Complicações na Gravidez/sangue , Prognóstico , Estudos Prospectivos , Estações do Ano , Deficiência de Vitamina D/sangue
11.
Zhonghua Liu Xing Bing Xue Za Zhi ; 40(2): 180-185, 2019 Feb 10.
Artigo em Chinês | MEDLINE | ID: mdl-30744269

RESUMO

Objective: To study the dose-response relationship between maternal thyroid hormone levels in the first twenty weeks of pregnancy and the infant physical and neuropsychological development. Methods: In this prospective cohort study, a total of 945 women and their children were included. Maternal serum samples during first half of the pregnancy were collected and analyzed for levels of thyroid hormones by using the electro-chemiluminescence immunoassay. Maternal social demographic information was collected by using the a self-administered questionnaire. Physical measurements of newborns and neuropsychological evaluation of infants were performed by doctors of maternal and child health care. Results: The differences in newborns' birth length and head circumference were significant among the newborns of mothers with different percentiles of maternal serum (thyroid-stimulating hormone, TSH) levels (P<0.05). Newborns with maternal TSH level ≥P(95) or

Assuntos
Peso ao Nascer/fisiologia , Desenvolvimento Infantil/fisiologia , Hormônios Tireóideos/sangue , Tireotropina/sangue , Criança , China , Feminino , Sangue Fetal/metabolismo , Humanos , Lactente , Recém-Nascido/sangue , Gravidez , Efeitos Tardios da Exposição Pré-Natal/sangue , Estudos Prospectivos , Glândula Tireoide/fisiologia , Hormônios Tireóideos/metabolismo
12.
Acta Diabetol ; 56(4): 421-430, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30725264

RESUMO

AIMS: Maternal obesity and gestational diabetes mellitus (GDM) were frequently reported to be risk factors for obesity and diabetes in offspring. Our goal was to study the impact of maternal prepregnancy BMI (pBMI) and GDM on both maternal and cord blood metabolic profiles. METHODS: We used LC-MS/MS to measure 201 metabolites comprising phospholipids (PL), amino acids, non-esterified fatty acids (NEFA), organic acids, acyl carnitines (AC), and Krebs cycle metabolites in maternal plasma at delivery and cord plasma obtained from 325 PREOBE study participants. RESULTS: Several metabolites were associated with pBMI/GDM in both maternal and cord blood (p < 0.05), while others were specific to either blood sources. BMI was positively associated with leucine, isoleucine, and inflammation markers in both mother and offspring, while ß-hydroxybutyric acid was positively associated only in cord blood. GDM showed elevated levels of sum of hexoses, a characteristic finding in both maternal and cord blood. Uniquely in cord blood of offspring born to GDM mothers, free carnitine was significantly lower with the same tendency observed for AC, long-chain NEFA, PL, specific Krebs cycle metabolites, and ß-oxidation markers. CONCLUSIONS: Maternal BMI and GDM are associated with maternal and cord blood metabolites supporting the hypothesis of transgenerational cycle of obesity and diabetes.


Assuntos
Índice de Massa Corporal , Diabetes Gestacional/metabolismo , Sangue Fetal/metabolismo , Metaboloma , Adulto , Cromatografia Líquida , Estudos de Coortes , Ácidos Graxos/metabolismo , Ácidos Graxos não Esterificados/metabolismo , Feminino , Humanos , Recém-Nascido , Masculino , Obesidade/complicações , Obesidade/metabolismo , Fosfolipídeos/metabolismo , Gravidez , Complicações na Gravidez/metabolismo , Efeitos Tardios da Exposição Pré-Natal/metabolismo , Fatores de Risco , Espectrometria de Massas em Tandem
13.
J Pregnancy ; 2019: 5062365, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30693107

RESUMO

Background: Preterm birth is still a global burden particularly in Indonesia. The suboptimal concentration of certain micronutrients and heavy metals is hypothesized to play a role in the mechanism of preterm birth. Objective: This study aimed to analyze the micronutrients and heavy metals concentrations between subjects with term and preterm birth. Design: A cross-sectional study was conducted during January-June 2017 in Cipto Mangunkusumo Hospital and Budi Kemuliaan Hospital, Jakarta, Indonesia. Subjects were divided into term and preterm birth groups. The measured outcomes were maternal serum, placental, and blood cord concentration of zinc, copper, iron, selenium, manganese, mercury, lead, AtRA, and 25(OH)D. Results: A total of 51 pregnant women participated in this study. Term group had higher concentration of maternal serum AtRA (0.22 ± 0.07 ng/mL versus 0.12 ± 0.03 ng/mL, p <0.001), higher placental concentration of manganese {0.99 (0.38 - 1.78) µg/g versus 0.42 ± 0.18 µg/g, p <0.001}, iron (252.16 ± 170.61 µg/g versus 78.45 ± 51.73 µg/g, p <0.001), copper {2.96 ± 1.80 µg/g versus 1.62 (0.70 - 3.88) µg/g, p 0.019}, zinc {58.34 (27.88 - 124.05) µg/g versus 28.41 (1.46 - 137.69) µg/g, p 0.011}, selenium (0.31 ± 0.31 ng/g versus 0.14 ± 0.20 ng/g, p 0.024), AtRA {21.7 ± 10.69 ng/g versus 0.7 (0.42 - 5.10) ng/g, p <0.001}, and 25(OH)D {75.84 ± 45.12 ng/g versus 18.00 (5 - 88) ng/g, p <0.001}, lower placental concentration of mercury (0.20 ± 0.17 ng/g versus 20.47 ± 41.35 ng/g, p 0.019) and lead (0.02 ± 0.01 ng/g versus 0.81 ± 1.43 ng/g, p 0.009), and higher cord blood concentration of copper {32.20 (16.30 - 69.60) µg/dL versus 20.60 (5.80 - 53.30) µg/dL, p 0.006} and AtRA (0.16 ± 0.04 versus 0.07 ± 0.01, p <0.001). Conclusion: Preterm birth is associated with lower concentrations of micronutrients which play a role in antioxidant mechanism, as well as higher concentration of mercury and lead.


Assuntos
Sangue Fetal/metabolismo , Metais Pesados/sangue , Metais Pesados/metabolismo , Micronutrientes/sangue , Micronutrientes/metabolismo , Placenta/metabolismo , Gravidez/sangue , Gravidez/metabolismo , Nascimento Prematuro/etiologia , Nascimento Prematuro/metabolismo , Antioxidantes , Estudos Transversais , Feminino , Humanos , Indonésia , Chumbo/sangue , Chumbo/metabolismo , Mercúrio/sangue , Mercúrio/metabolismo , Nascimento Prematuro/sangue
14.
Biomolecules ; 9(1)2019 01 21.
Artigo em Inglês | MEDLINE | ID: mdl-30669674

RESUMO

Previous studies suggest that children who progress to type 1 diabetes (T1D) later in life already have an altered serum lipid molecular profile at birth. Here, we compared cord blood lipidome across the three study groups: children who progressed to T1D (PT1D; n = 30), children who developed at least one islet autoantibody but did not progress to T1D during the follow-up (P1Ab; n = 33), and their age-matched controls (CTR; n = 38). We found that phospholipids, specifically sphingomyelins, were lower in T1D progressors when compared to P1Ab and the CTR. Cholesterol esters remained higher in PT1D when compared to other groups. A signature comprising five lipids was predictive of the risk of progression to T1D, with an area under the receiver operating characteristic curve (AUROC) of 0.83. Our findings provide further evidence that the lipidomic profiles of newborn infants who progress to T1D later in life are different from lipidomic profiles in P1Ab and CTR.


Assuntos
Diabetes Mellitus Tipo 1/patologia , Sangue Fetal/metabolismo , Ilhotas Pancreáticas/imunologia , Lipídeos/análise , Área Sob a Curva , Autoanticorpos/análise , Estudos de Casos e Controles , Criança , Pré-Escolar , Cromatografia Líquida de Alta Pressão , Análise Discriminante , Progressão da Doença , Feminino , Humanos , Análise dos Mínimos Quadrados , Masculino , Espectrometria de Massas , Curva ROC , Esfingomielinas/metabolismo
16.
Nutrients ; 11(1)2019 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-30634589

RESUMO

Lutein + zeaxanthin (L + Z) are carotenoids recognized in eye health, but less is known about their status during pregnancy. While quantified in maternal and umbilical cord blood, they have never been analyzed in placenta. The purpose of this study is to quantify combined L + Z concentrations in human placenta and correlate with levels in maternal dietary intake, maternal serum, and umbilical cord blood. The proportions of combined L + Z were compared within diet, placenta, maternal serum, and umbilical cord blood among additional carotenoids (lycopene, ß-cryptoxanthin, α-carotene, and ß-carotene). This Institutional Review Boardapproved cross-sectional study enrolled 82 mother-infant pairs. Placenta, maternal serum, and umbilical cord blood samples were analyzed for carotenoids concentrations. Mothers completed a food frequency questionnaire and demographic/birth outcome data were collected. L + Z were present in placenta, median 0.105 micrograms/gram (mcg/g) and were significantly correlated with maternal serum (r = 0.57; p < 0.001), umbilical cord blood levels (r = 0.49; p = 0.001), but not dietary intake (p = 0.110). L + Z were the most prevalent in placenta (49.1%) umbilical cord blood (37.0%), but not maternal serum (18.6%) or dietary intake (19.4%). Rate of transfer was 16.0%, the highest of all carotenoids. Conclusively, L + Z were identified as the two most prevalent in placenta. Results highlight unique roles L + Z may play during pregnancy.


Assuntos
Dieta , Sangue Fetal/metabolismo , Luteína/sangue , Fenômenos Fisiológicos da Nutrição Materna , Zeaxantinas/sangue , Adulto , beta-Criptoxantina/sangue , Carotenoides/sangue , Estudos Transversais , Inquéritos sobre Dietas , Feminino , Humanos , Recém-Nascido , Licopeno/sangue , Masculino , Placenta , Gravidez , Xantofilas/sangue , Adulto Jovem , beta Caroteno/sangue
17.
Transfusion ; 59(2): 681-685, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30537299

RESUMO

BACKGROUND: Umbilical cord blood (UCB) has become an important source of transplantable CD34+ hematopoietic progenitor cells. Cord blood banks (CBBs) can increase their efficiency by minimizing the processing of UCB units with low CD34+ content, which have a lower likelihood of transplant utilization. We sought to identify a readily available preprocessing metric that would correlate with CD34+ cell counts, without the cost of additional analysis. STUDY DESIGN AND METHODS: Data were compiled for 131 UCB units processed at the regional CBB. Preprocessing hematologic metrics, including complete blood count and differential, were compared to postprocessing CD34+ cell quantities. The data were divided into six groups of varying preprocessing metrics, then compared for significant differences in postprocessing CD34+ cell quantities to develop a screening guidance. RESULTS: UCB units with nucleated RBC (nRBC) content of 15% or greater were found to have a significant increase in CD34+ cell percentage (p < 0.00001) and total CD34+ cell content (p < 0.0001). Units with preprocessing total nucleated cell count (TNC) of ≥ 1.50 × 109 with nRBC content of 15% or greater, and for TNC ≥ of 2.00 × 109 with nRBC content less than15%, had a significant increase in CD34+ content (p < 0.05 and p < 0.001, respectively). Applied as a screening guideline, these units had an increase in mean CD34+ content from 6.24 × 106 to 9.27 × 106 . Units originally in the bottom and top quartiles of CD34+ content constitute 5% and 53% of processed units meeting these TNC/nRBC criteria, respectively. CONCLUSION: These screening criteria utilizing nRBC provides a guideline that public CBBs may use to increase their efficiency by minimizing the processing of UCB units with lower CD34+ cell content.


Assuntos
Antígenos CD34/sangue , Bancos de Sangue , Sangue Fetal/citologia , Células-Tronco Hematopoéticas/citologia , Transplante de Células-Tronco de Sangue do Cordão Umbilical , Contagem de Eritrócitos , Sangue Fetal/metabolismo , Células-Tronco Hematopoéticas/metabolismo , Humanos , Contagem de Leucócitos
18.
Pediatr Int ; 61(2): 140-146, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30565800

RESUMO

BACKGROUND: We investigated the association between the hormone environment during the prenatal period using cord blood, and gender-role play behavior in school-aged children. METHODS: A total of 879 school-aged children (433 boys and 446 girls) in a prospective birth cohort study in Hokkaido were enrolled to analyze the relationship between cord blood level of the sex hormones estradiol (E), testosterone (T), progesterone (P), and dehydroepiandrosterone (DHEA), and the Pre-School Activities Inventory (PSAI) score. The PSAI evaluated sex-typical characteristics, the type of preferred toys and play activities. The PSAI consists of 12 masculine and 12 feminine items, and the composite scores were calculated by subtracting the feminine score from the masculine score. Higher scores indicated male-typical behavior. RESULTS: Composite and masculine PSAI scores were significantly higher in boys. Meanwhile, the feminine score was significantly lower in boys. Although T and P were significantly higher in boys, E/T was significantly higher in girls. In a multivariate regression model, including covariates of social factors, there was no correlation between any of the hormones and PSAI score in boys. In girls, only P and E/T were positively correlated with the feminine score. CONCLUSIONS: Prenatal sex hormone exposure may influence the dimorphic brain development and behavior in school-aged girls. Furthermore, the cord blood hormone levels may not fully reflect the hormone environment during the prenatal period.


Assuntos
Comportamento Infantil/fisiologia , Sangue Fetal/metabolismo , Identidade de Gênero , Hormônios Esteroides Gonadais/sangue , Jogos e Brinquedos/psicologia , Efeitos Tardios da Exposição Pré-Natal/sangue , Biomarcadores/sangue , Criança , Comportamento Infantil/psicologia , Feminino , Humanos , Masculino , Gravidez , Efeitos Tardios da Exposição Pré-Natal/psicologia , Estudos Prospectivos
19.
J Chromatogr A ; 1586: 30-39, 2019 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-30563692

RESUMO

This paper describes the development of an original micro-solid phase extraction device and its evaluation for the isolation of F2-isoprostanes (F2-IsoPs) from cord and maternal plasma samples. The unit is very simple and consists in a rotating disc (1.8 cm diameter) of oxidized buckypaper (BP), enwrapped in a polypropylene mesh pouch. Even if the selected F2-IsoPs have logP and pKa values that make them suitable candidates for their sorption on BP, several parameters were optimized to maximize recoveries: time of adsorption and desorption; stirring speed; volume, pH and ionic strength of the sample; type, volume, and fractions of the elution solvent; oxidation grade of BP. Among all, the last one was crucial in affecting extraction yields because of the analyte interactions with polar functionalities, introduced by a preliminary oxidative acid treatment. The investigation established the optimal oxidation time and highlighted the pros and cons of the acid activation step. All extracts were analyzed by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Validation was performed according to the main FDA guidelines for bioanalytical methods. Depending on the spike level and analyte, recoveries ranged between 30 and 120% with precision and accuracy values lower than 20%. Quantitative analysis was accomplished by matrix-matched calibration curves whose determination coefficients were higher than 0.95. Lower limit of quantitation (LLOQ) spanned the range 2.45-6.77 µg L-1. The validated method was applied to the analysis of eight pairs of mother/child plasma samples, revealing the presence of 8-iso-15-keto-PGF2α and 8-iso-PGE2 at a concentration of about 10 µg L-1 in most cord plasma samples of preterm newborns.


Assuntos
F2-Isoprostanos/análise , F2-Isoprostanos/isolamento & purificação , Sangue Fetal/metabolismo , Nanotubos de Carbono/química , Papel , Extração em Fase Sólida/métodos , Adsorção , Feminino , Humanos , Recém-Nascido , Limite de Detecção , Gravidez , Solventes , Espectrometria de Massas em Tandem/métodos
20.
Artigo em Inglês | MEDLINE | ID: mdl-30529256

RESUMO

OBJECTIVE: Erythropoietin - a hormone regulating erythropoiesis - is a biomarker of chronic fetal hypoxia. High erythropoietin levels in fetal plasma and amniotic fluid are associated with increased risk of adverse neonatal outcome. Since the risk of perinatal morbidity and mortality is increased in pregnancies beyond 41 gestational weeks, we evaluated erythropoietin levels in amniotic fluid and umbilical cord serum in apparently low-risk term (≥ 37 gestational weeks) and prolonged pregnancies (≥ 41 gestational weeks) with labor induction. STUDY DESIGN: This prospective cohort study comprised 93 singleton pregnancies at 37+0-42+1 gestational weeks, of which prolonged pregnancies numbered 63 (67.7%). Amniotic fluid samples were collected at time of labor induction by amniotomy. Umbilical cord blood samples for evaluation of pH, base excess, and umbilical cord serum erythropoietin were collected at birth. Erythropoietin levels were measured by immunochemiluminometric assay. Normal value of amniotic fluid erythropoietin level was defined as ≤ 3 IU/L, and abnormal value as ≥ 27 IU/L. Normal umbilical cord serum erythropoietin was defined as < 40 IU/L. Data on maternal pregnancy and delivery characteristics and short-term neonatal outcomes such as Apgar score were obtained from the hospital charts. Associations were calculated using Spearman's rank correlation coefficient. The Chi-square test, Fisher's exact test and the Mann-Whitney U test were utilized to determine differences in the study groups. RESULTS: Amniotic fluid erythropoietin levels correlated with gestational age (r = 0.261, p = 0.012) and were higher among prolonged pregnancies as compared to term pregnancies (p = 0.005). There were 78 (83.9%) vaginal deliveries, and among these erythropoietin levels in amniotic fluid correlated with the levels in umbilical cord serum (r = 0.513, p < 0.000). Umbilical cord serum erythropoietin levels correlated with gestational age among vaginal deliveries (r = 0.250, p = 0.027). Erythropoietin levels in amniotic fluid and umbilical cord serum did not correlate with umbilical artery pH or base excess, or other adverse pregnancy outcome. CONCLUSIONS: In vaginal deliveries erythropoietin levels in amniotic fluid correlated with the levels in umbilical cord serum. Erythropoietin levels correlated with gestational age, probably due to weakening placental function and relative hypoxemia occurring in advanced gestation. However, in this relatively low-risk study population erythropoietin was not related to adverse delivery outcome.


Assuntos
Líquido Amniótico/metabolismo , Eritropoetina/sangue , Sangue Fetal/metabolismo , Gravidez Prolongada/sangue , Nascimento a Termo/sangue , Adulto , Biomarcadores/sangue , Estudos de Casos e Controles , Parto Obstétrico/estatística & dados numéricos , Feminino , Hipóxia Fetal/sangue , Hipóxia Fetal/diagnóstico , Humanos , Gravidez , Resultado da Gravidez , Estudos Prospectivos , Estatísticas não Paramétricas
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