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1.
Int J Nanomedicine ; 18: 17-34, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36628114

RESUMO

Introduction: A number of biological wastes and factory waste materials have been tested recently for the eco-friendly biosynthesis of nanoparticles. Sericin protein (SSP) is usually removed from the silk cocoon during the degumming process in the process of making the silk, and this sericin protein is normally thrown away by the sericulture industries as waste materials. It is found that this sericin protein possesses a number of biological properties. Methods: Considering this, in the present study, an effort has been made to biosynthesize gold nanoparticles (SSP-AuNPs) using the waste sericin solution as the reducing and capping agent and investigate its biopotential in terms of its wound healing, antioxidant and antibacterial activities. Results: The synthesis of SSP-AuNPs was perceived by the visual color change and confirmed by UV-Vis spectroscopy with absorption maxima at 522 nm. Further characterization of SSP-AuNPs was done by TEM, EDS, XRD, FTIR, DLS, zeta potential, TGA, AFM, etc. The size of SSP-AuNPs was found out to be 54.82 nm as per the particle size analyzer and the zeta potential is -19.8 mV. The SSP-AuNPs displayed promising wound healing potential of 70.96 and 69.76% wound closure rate at 5 and 10 µg/mL respectively as compared to 74.91% by the Centella asiatica taken as a positive control. It also exhibited promising antioxidant potential in terms of the DPPH, ABTS free radical scavenging, reducing power potential, and total antioxidant capacity. Besides, the SSP-AuNPs also displayed significant antibacterial activities against the tested pathogenic bacterial with the diameter of inhibition zones ranging between 12.10 and 14.96 mm as compared to the positive control cephalexin that displayed inhibition zones ranging between 12.08 and 13.24 mm. Discussion: Taken together, SSP-AuNPs could serve as an interesting candidate for food, cosmetics, and biomedical fields in the applications of wound healing, cosmetics, antibacterial bandages, and ointments, etc.


Assuntos
Bombyx , Nanopartículas Metálicas , Sericinas , Animais , Seda/química , Antioxidantes/farmacologia , Antioxidantes/química , Sericinas/química , Sericinas/farmacologia , Bombyx/química , Ouro/química , Nanopartículas Metálicas/química , Antibacterianos/farmacologia , Antibacterianos/química , Cicatrização
2.
J Exp Biol ; 226(2)2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36633333

RESUMO

To successfully capture flying insect prey, a spider's orb web must withstand the energy of impact without the silk breaking. In this study, we examined the anchor threads: the silk lines that anchor the main capture area of the web to the surrounding environment. These anchor threads can account for a large portion of the web, yet are usually excluded from experiments and simulations. We compared projectile capture and kinetic energy absorption between webs with and without access to anchor threads. Webs with anchor threads captured significantly more projectiles and absorbed significantly more energy than those with constrained anchors. This is likely because the anchor threads increase web compliance, resulting in webs with the ability to catch high-energy flying insects without breaking. Anchor threads are one example of how different types of web architecture expand the range of possible prey capture strategies by enabling the web to withstand greater impacts.


Assuntos
Aranhas , Animais , Comportamento Predatório , Seda , Cinética
3.
Int J Mol Sci ; 24(2)2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36674467

RESUMO

This study illustrates the sensing and wound healing properties of silk fibroin in combination with peptide patterns, with an emphasis on the printability of multilayered grids, and envisions possible applications of these next-generation silk-based materials. Functionalized silk fibers covalently linked to an arginine-glycine-aspartic acid (RGD) peptide create a platform for preparing a biomaterial ink for 3D printing of grid-like piezoresistors with wound-healing and sensing properties. The culture medium obtained from 3D-printed silk fibroin enriched with RGD peptide improves cell adhesion, accelerating skin repair. Specifically, RGD peptide-modified silk fibroin demonstrated biocompatibility, enhanced cell adhesion, and higher wound closure rates at lower concentration than the neat peptide. It was also shown that the printing of peptide-modified silk fibroin produces a piezoresistive transducer that is the active component of a sensor based on a Schottky diode harmonic transponder encoding information about pressure. We discovered that such biomaterial ink printed in a multilayered grid can be used as a humidity sensor. Furthermore, humidity activates a transition between low and high conductivity states in this medium that is retained unless a negative voltage is applied, paving the way for utilization in non-volatile organic memory devices. Globally, these results pave the way for promising applications, such as monitoring parameters such as human wound care and being integrated in bio-implantable processors.


Assuntos
Fibroínas , Materiais Inteligentes , Humanos , Seda/química , Fibroínas/química , Tinta , Materiais Biocompatíveis/química , Cicatrização , Peptídeos , Impressão Tridimensional
4.
Molecules ; 28(2)2023 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-36677907

RESUMO

Major progress has been made in cancer research; however, cancer remains one of the most important health-related burdens. Sericulture importance is no longer limited to the textile industry, but its by-products, such as silk fibroin or mulberry, exhibit great impact in the cancer research area. Fibroin, the pivotal compound that is found in silk, owns superior biocompatibility and biodegradability, representing one of the most important biomaterials. Numerous studies have reported its successful use as a drug delivery system, and it is currently used to develop three-dimensional tumor models that lead to a better understanding of cancer biology and play a great role in the development of novel antitumoral strategies. Moreover, sericin's cytotoxic effect on various tumoral cell lines has been reported, but also, it has been used as a nanocarrier for target therapeutic agents. On the other hand, mulberry compounds include various bioactive elements that are well known for their antitumoral activities, such as polyphenols or anthocyanins. In this review, the latest progress of using sericultural by-products in cancer therapy is discussed by highlighting their notable impact in developing novel effective drug strategies.


Assuntos
Antineoplásicos , Bombyx , Fibroínas , Neoplasias , Animais , Humanos , Bombyx/metabolismo , Antocianinas , Seda , Fibroínas/metabolismo , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Neoplasias/tratamento farmacológico
5.
Sensors (Basel) ; 23(2)2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36679667

RESUMO

Cocoon sorting is one of the most labor-demanding activities required both at the end of the agricultural production and before the industrial reeling process to obtain an excellent silk quality. In view of the possible relaunch of European sericulture, the automatization of this production step is mandatory both to reduce silk costs and to standardize fiber quality. The described research starts from this criticality in silk production (the manual labor required to divide cocoons into different quality classes) to identify amelioration solutions. To this aim, the automation of this activity was proposed, and a first prototype was designed and built. This machinery is based on the use of three cameras and imaging algorithms identifying the shape and size of the cocoons and outside stains, a custom-made light sensor and an AI model to discard dead cocoons. The current efficiency of the machine is about 80 cocoons per minute. In general, the amelioration obtained through this research involves both the application of traditional sensors/techniques to an unusual product and the design of a dedicated sensor for the identification of dead/alive pupae inside the silk cocoons. A general picture of the overall efficiency of the new cocoon-sorting prototype is also outlined.


Assuntos
Bombyx , Animais , Seda
6.
PLoS Genet ; 19(1): e1010602, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36652497

RESUMO

Silkworm silk gland cells undergo endoreplicating cycle and rapid growth during the larval period, and synthesize massive silk proteins for silk production. In this study, we demonstrated that a binary transgenic CRISPR/Cas9 approach-mediated Fzr mutation in silkworm posterior silk gland (PSG) cells caused an arrest of silk gland growth and a decrease in silk production. Mechanistically, PSG-specific Fzr mutation blocked endoreplication progression by inducing an expression dysregulation of several cyclin proteins and DNA replication-related regulators. Moreover, based on label-free quantitative proteome analysis, we showed in PSG cells that Fzr mutation-induced decrease in the levels of cyclin proteins and silk proteins was likely due to an inhibition of the ribosome biogenesis pathway associated with mRNA translation, and/or an enhance of the ubiquitin-mediated protein degradation pathway. Rbin-1 inhibitor-mediated blocking of ribosomal biogenesis pathway decreased DNA replication in PSG cells and silk production. Altogether, our results reveal that Fzr positively regulates PSG growth and silk production in silkworm by promoting endoreplication and protein synthesis in PSG cells.


Assuntos
Bombyx , Animais , Endorreduplicação , Seda/genética , Biossíntese de Proteínas/genética , Ciclinas/genética , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo
7.
ACS Appl Bio Mater ; 6(1): 74-82, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36603189

RESUMO

Transdermal drug delivery is an attractive option for multiple disease therapies as it reduces adverse reactions and improves patient compliance. Water-dispersible ß-sheet rich silk nanofiber carriers have hydrophobic properties that benefit transdermal delivery but still show inferior transdermal capacities. Thus, hydrophobic silk nanofibers were fabricated to fine-tune their size and endow them with desirable transdermal delivery capacities. Silk nanocarrier length was shortened from 2000 nm to approximately 40 nm after ultrasonic treatment. In vitro human skin and in vivo animal studies revealed different transdermal behaviors for silk nanocarriers at different nanosizes. Silk nanocarriers passed slowly through the corneum without destroying the corneum structure. Improved transdermal capacity was achieved for smaller size carriers. Both hydrophilic and hydrophobic drugs could be loaded onto silk nanocarriers, suggesting a promising future for different disease therapies. No cytotoxicity and skin irritation were identified for silk nanocarriers, which strengthened their superiority as transdermal carriers. Therefore, silk nanocarriers <100 nm may promote the percutaneous absorption of active cargos for disease therapy and cosmetic applications.


Assuntos
Seda , Pele , Animais , Humanos , Seda/química , Administração Cutânea , Pele/metabolismo , Absorção Cutânea , Portadores de Fármacos/química
8.
Drug Deliv ; 30(1): 2168793, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36694964

RESUMO

Guava (Psidium guajava L.) is a well-known plant containing high levels of natural antioxidants, the phenolic compounds, which have been employed in numerous cosmetic products. However, these molecules are unstable to oxidants, light, temperature, pH, water, and enzymatic activities. Therefore, to enhance their stability and preserve their antioxidant activity, this study investigated the silk fibroin nanoparticles (SFNs) ability to encapsulate, deliver, and heat-protect the phenolic compounds of the guava leaves ethanolic extract. Firstly, the guava ethanolic extract was produced by maceration, which possessed a total phenolic content of 312.6 mg GAE/g DPW and a high antioxidant activity (IC50 = 5.397 ± 0.618 µg/mL). Then, the extract loaded SFNs were manufactured by desolvation method, and the particles demonstrated appropriate sizes of 200-700 nm with narrow size distribution, spherical shape, silk-II crystalline structure, high drug entrapment efficiency of > 70% (dependent on the fibroin content), and a two-phase sustained drug release for at least 210 min. Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the antioxidant activity of the guava extract was well-preserved in the extract loaded SFNs. Finally, after being treated with high temperature of 70 °C for 24 h, the guava extract almost loses all of its antioxidant property (5 times decrement), whereas the extract loaded SFNs could retain the extract activity. Conclusively, the SFNs proved much potential to deliver and heat-protect the guava extract phenolic compounds, and preserve their antioxidant activity. Confirmed by this case, SFNs could be further explored in protecting other natural compounds from environmental factors.


Assuntos
Fibroínas , Nanopartículas , Psidium , Antioxidantes/química , Psidium/química , Seda , Fenóis , Extratos Vegetais/química , Folhas de Planta/química
9.
Nat Commun ; 14(1): 210, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36639727

RESUMO

Volumetric additive manufacturing (VAM) enables fast photopolymerization of three-dimensional constructs by illuminating dynamically evolving light patterns in the entire build volume. However, the lack of bioinks suitable for VAM is a critical limitation. This study reports rapid volumetric (bio)printing of pristine, unmodified silk-based (silk sericin (SS) and silk fibroin (SF)) (bio)inks to form sophisticated shapes and architectures. Of interest, combined with post-fabrication processing, the (bio)printed SS constructs reveal properties including reversible as well as repeated shrinkage and expansion, or shape-memory; whereas the (bio)printed SF constructs exhibit tunable mechanical performances ranging from a few hundred Pa to hundreds of MPa. Both types of silk-based (bio)inks are cytocompatible. This work supplies expanded bioink libraries for VAM and provides a path forward for rapid volumetric manufacturing of silk constructs, towards broadened biomedical applications.


Assuntos
Bioimpressão , Fibroínas , Seda , Tinta , Bioimpressão/métodos , Impressão Tridimensional , Engenharia Tecidual/métodos , Tecidos Suporte
10.
Carbohydr Polym ; 304: 120479, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36641188

RESUMO

Chitosan foams are among the approved hemostats for pre-hospital hemorrhagic control but suffer from drawbacks related to mucoadhesiveness and rebleeding. Herein, we have developed a designer bilayered hemostatic foam consisting of a bioactive layer composed of silica particles (≈300 nm) and silk fibroin to serve as the tissue interfacing component on a chitosan foam. The foam composition was optimized based on the in vitro clotting behavior and cytocompatibility of individual components. In vivo analysis in a rat model demonstrated that the developed hemostat could achieve rapid clotting (31 ± 4 s), similar to a chitosan-based hemostat, but the former had significantly lower blood loss. Notably, removal of the bilayered hemostat prevented rebleeding, unlike the chitosan foam, which was associated with markedly higher incidences of rebleeding (50 %) and left behind material residue. Thus, the designer bilayered foam presented here is a potent inducer of blood clotting whilst affording easy removal with minimal rebleeding.


Assuntos
Quitosana , Fibroínas , Hemostáticos , Ratos , Animais , Quitosana/química , Seda , Hemostáticos/farmacologia , Coagulação Sanguínea , Hemostasia , Fibroínas/química , Hemorragia/tratamento farmacológico
11.
Int J Biol Macromol ; 224: 1412-1422, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36550790

RESUMO

Superelastic silk fibroin (SF)-based aerogels can be used as multifunctional substrates, exhibiting a promising prospect in air filtration, thermal insulation, and biomedical materials. However, fabrication of the superelastic pure SF aerogels without adding synthetic polymers remains challenging. Here, the SF micro-nano fibrils (SMNFs) that preserved mesostructures are extracted from SF fibers as building blocks of aerogels by a controllable deep eutectic solvent liquid exfoliation technique. SMNFs can assemble into multiscale fibril networks during the freeze-inducing process, resulting in all-natural SMNF aerogels (SMNFAs) with hierarchical cellular architectures after lyophilization. Benefiting from these structural features, the SMNFAs demonstrate desirable properties including ultra-low density (as low as 4.71 mg/cm3) and superelasticity (over 85 % stress retention after 100 compression cycles at 60 % strain). Furthermore, the potential applications of superelastic SMNFAs in air purification and thermal insulation are investigated to exhibit their functionality, mechanical elasticity, and structural stability. This work provides a reliable approach for the fabrication of highly elastic SF aerogels and endows application prospects in air purification and thermal insulation opportunities.


Assuntos
Fibroínas , Seda , Fibroínas/química , Solventes Eutéticos Profundos , Géis/química , Materiais Biocompatíveis
12.
Biochem Biophys Res Commun ; 640: 117-124, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36502627

RESUMO

Repairing skin wounds has always been challenging in clinical practice. The new skin tissue engineering scaffold provides innovative ways to address these challenges with a good chance of success because of its stable mechanical properties, biodegradability, and antibacterial properties. This paper presents the fabrication and evaluation of a three-dimensional composite scaffold made with sulfated silk fibroin, chitosan, and hydroxyapatite (SSF/CS/HAP). An electron microscope shows that the scaffold has an aperture of 15-20 µm, while an absorption performance test shows that its expansion index reaches 779%. The co-culture of L929 cells and the CCK-8 experiments demonstrated good cell compatibility and low scaffold cytotoxicity, respectively. Meanwhile, in vivo experiments demonstrate that rats with SSF/CS/HAP scaffold-treated neck wounds heal faster. In the wound skin tissue of the SSF/CS/HAP scaffold group, immunohistochemistry indicates a more rapid and mature development of hair follicles. This study successfully developed a novel skin tissue engineering scaffold material with high moisture retention, high tissue compatibility, and low cytotoxicity, demonstrating its ability to improve wound repair with promising potential for tissue engineering applications.


Assuntos
Quitosana , Fibroínas , Ratos , Animais , Engenharia Tecidual/métodos , Fibroínas/química , Quitosana/química , Durapatita/química , Sulfatos , Tecidos Suporte/química , Seda/química , Materiais Biocompatíveis/química
13.
Biomacromolecules ; 24(1): 332-343, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36562543

RESUMO

Natural fiber-reinforced biocomposites with excellent mechanical and biological properties have attractive prospects for internal medical devices. However, poor interfacial adhesion between natural silk fiber and the polymer matrix has been a disturbing issue for such applications. Herein, rigid-flexible agents, such as polydopamine (PDA) and epoxy soybean oil (ESO), were introduced to enhance the interfacial adhesion between Antheraea pernyi (Ap) silk and a common medical polymer, polycaprolactone (PCL). We compared two strategies of depositing PDA first (Ap-PDA-ESO) and grafting ESO first (Ap-ESO-PDA). The rigid-flexible interfacial agents introduced multiple molecular interactions at the silk-PCL interface. The "Ap-PDA-ESO" strategy exhibited a greater enhancement in interfacial adhesion, and interfacial toughening mechanisms were proposed. This work sheds light on engineering strong and tough silk fiber-based biocomposites for biomedical applications.


Assuntos
Polímeros , Seda , Poliésteres
14.
Int J Biol Macromol ; 228: 261-272, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36581022

RESUMO

Developing a functional coating for vascular stents with sustainable and tunable NO release remains challenging. In this work, we report a silk fibroin/chitosan-based biopolymer coating incorporating copper ions as a catalyst for NO generation and demonstrate its potential for the surface functionalization of cardiovascular stents. Based on the differences in silk fibroin and chitosan coordinating with copper ions, the loading, bonding, and release of copper ions could be precisely regulated over a wide range by controlling the ratio of silk fibroin and chitosan. This system shows good cytocompatibility for endothelial cells and tunable catalytic activity to decompose S-nitroso-N-acetyl-D-penicillamine (SNAP) for NO generation. Consequently, a functionalized coating with sustainable and tunable NO catalysis generation was developed on the metallic stent. Based on good biocompatibility, tunable NO release, and simple processing, the coating is expected to have great promise in the field of intervention therapy of cardiovascular disease.


Assuntos
Quitosana , Fibroínas , Óxido Nítrico , Células Endoteliais , Cobre , Stents , Seda
15.
Biomater Adv ; 145: 213224, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36516618

RESUMO

A single system capable of delivering anticancer drugs and growth factors by a minimally invasive approach is in demand for effective treatment of triple-negative breast cancer (TNBC) after lumpectomy. Here, we showcase one such holistic system for TNBC therapy and its assessment via 3D in vitro lumpectomy model, a first of its kind. Firstly, Bombyx mori silk fibroin (BMSF) and Antheraea assamensis silk fibroin (AASF) blended hydrogels were prepared and biophysically characterized. Secondly, a 3D in vitro lumpectomy model was developed using MDA-MB-231 cell line to assess the efficacy of localized delivery of doxorubicin (dox) using injectable hydrogel system in terminating remaining breast cancer after lumpectomy. Additionally, we have also evaluated the adipose tissue regeneration in the lumpectomy region by delivering dexamethasone (dex) using injectable hydrogels. Rheological studies showed that the BMSF/AASF blended hydrogels exhibit viscoelasticity and injectability conducive for minimally invasive application. The developed hydrogels by virtue of its slow and sustained release of dox exerted cytotoxicity towards MDA-MB-231 cells assessed through in vitro studies. Further, dex loaded hydrogel supported adipogenic differentiation of adipose tissue derived stem cells (ADSCs), while the secreted factors were found to aid in vascularization and macrophage polarization. This was confirmed through in vitro angiogenic tube formation assay and macrophage polarization study respectively. The corroborated results vouch for potential application of this injectable hydrogels for localized anticancer drug delivery and aiding in breast reconstruction, post lumpectomy.


Assuntos
Antineoplásicos , Bombyx , Fibroínas , Mariposas , Neoplasias de Mama Triplo Negativas , Animais , Humanos , Seda , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Hidrogéis , Mastectomia Segmentar , Recidiva Local de Neoplasia , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Doxorrubicina/farmacologia , Doxorrubicina/uso terapêutico
16.
Poult Sci ; 102(2): 102360, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36512872

RESUMO

The current study aimed to investigate the dietary supplementation effect of corn silk (CS) on performance and blood chemistry of brown and white-feathered quails during the grower and layer periods. Japanese quails of brown and white-feathered color (180 birds/variety at 2 wks old) were randomly allotted into three groups with 3 replicates each (n = 20 birds/replicate). Corn silk powder (CS) was supplemented to the basal diet at 0, 1, and 2% of the diet for each quail variety for 1-month growing period, then continued for another 6-wk laying period to assess the egg production and egg quality characteristics. CS supplementation at 1% and 2% for brown and white-feathered quails respectively improved their growth performance (body weight and weight gain), carcass yield, and intestinal villi length with increasing feed consumption but without changes in feed conversion ratio. In both quail varieties, CS addition had a hypolipidemic effect, confirmed by lowering serum triglyceride (TG), cholesterol (CHO), and low density lipoprotein (LDL) while increased high density lipoprotein (HDL) concentrations (P < 0.05) with a clear response observed in white quails than the brown ones. Besides, CS supplementation increased (P = 0.002) hen day egg production in brown feathered quails, while reducing it in the white-feathered quails compared with the CS-free diet. The increased egg production was not significantly (P > 0.05) correlated with lower content of TG and CHO, while significantly increased the antioxidant content in both quail varieties (P < 0.05). Moreover, CS dietary supplementation significantly enhanced (P = 0.003) the yolk color, especially in brown-feathered quail. In conclusion, CS can be safely supplemented to the Japanese quail diet (1% and 2% for brown-feathered and white-feathered quails respectively) to improve growth performance, and egg quality characteristics.


Assuntos
Coturnix , Zea mays , Animais , Feminino , Coturnix/fisiologia , Galinhas , Suplementos Nutricionais , Dieta/veterinária , Codorniz , Colesterol , Seda , Ração Animal/análise
17.
Biomater Adv ; 145: 213223, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36502549

RESUMO

Biomaterials composed of silk fibroin from both mulberry and non-mulberry silkworm varieties have been investigated for their utility in tissue engineering and drug delivery, but these studies have largely excluded any evaluation of host immune response. The present study compares the macrophage activation response towards mulberry (Bombyx mori, BM) and non-mulberry (Antheraea assamensis, AA) silk types, individually and as a blend (BA) in a partial thickness rat abdominal wall defect model and in vitro primary murine bone marrow-derived macrophage (BMDM) assay. Biologic materials composed of liver extracellular matrix (LECM) and small intestinal submucosa (SIS) ECM that are recognized for constructive tissue remodeling, and polypropylene mesh that is associated with pro-inflammatory macrophage phenotype activation are used as controls in the animal model. The AA silk graft shows a host response similar to SIS with few foreign body multinucleate giant cells, vascularization, high CD206 expression, and high M2-like: M1-like macrophage phenotype ratio. Exposure to AA silk degradation products in vitro induces a higher arginase: iNOS ratio in both naive BMDM and pro-inflammatory activated BMDM; and higher Fizz1: iNOS ratio in pro-inflammatory activated BMDM. These data suggest that the AA silk supports a pro-remodeling macrophage response with potential therapeutic applications.


Assuntos
Bombyx , Fibroínas , Mariposas , Animais , Camundongos , Ratos , Bombyx/metabolismo , Ativação de Macrófagos , Seda/metabolismo , Mariposas/metabolismo
18.
Biotechnol Lett ; 45(2): 235-253, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36550336

RESUMO

Baicalein (BA) is a flavonoid with wide-ranging pharmacological activity. However, its biological evaluation is hampered by its low solubility in aqueous medium, making forms of incorporation that improve its solubility necessary. In the present study, BA was combined with a solution of silk fibroin protein (SF), a biomaterial used too as a drug carrier, to evaluate the anti-inflammatory potential of this combination, in vivo, in an experimental model, zebrafish (Danio rerio). Baicalein-silk fibroin (BASF) improved the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical scavenging rate (95%) in comparison with BA in solution. The acute toxicity study and histopathological analysis in zebrafish showed that BASF has low cytotoxic potential, except for the maxim dose of 2000 mg/kg. The use of BA in combination with SF enhanced the anti-inflammatory effect of flavonoids by inducing inflammatory peritoneal edema through carrageenan and achieved 77.6% inhibition of abdominal edema at a dose of 75 mg/kg. The results showed that the BASF, significantly increases the bioavailability and therapeutic effect of flavonoids and several results observed in this study may help in the development of new drugs.


Assuntos
Fibroínas , Animais , Fibroínas/farmacologia , Peixe-Zebra , Flavonoides , Anti-Inflamatórios/farmacologia , Edema/induzido quimicamente , Edema/tratamento farmacológico , Seda
19.
ACS Appl Bio Mater ; 6(1): 203-208, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36580433

RESUMO

Protein-based hydrogel biomaterials provide a platform for different biological applications, including the encapsulation and stabilization of different biomolecules. These hydrogel properties can be modulated by controlling the design parameters to match specific needs; thus, multicomponent hydrogels have distinct advantages over single-component hydrogels due to their enhanced versatility. Here, silk fibroin and γ-prefoldin chaperone protein based composite hydrogels were prepared and studied. Different ratios of the proteins were chosen, and the hydrogels were prepared by enzyme-assisted cross-linking. The secondary structure of the two proteins, dityrosine bond formation, and mechanical properties were assessed. The results obtained can be used as a platform for the rational design of composite thermostable hydrogel biomaterials to facilitate protection (due to hydrogel mechanics) and retention of bioactivity (e.g., of enzymes and other biomolecules) due to chaperone-like properties of γ-prefoldin.


Assuntos
Hidrogéis , Seda , Seda/química , Hidrogéis/química , Peroxidase do Rábano Silvestre/metabolismo , Materiais Biocompatíveis/química , Catálise
20.
Pestic Biochem Physiol ; 188: 105223, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36464330

RESUMO

Chlorantraniliprole (CAP) is widely used in the control of agricultural pests, and its residues can affect the formation of silkworm (Bombyx. mori) cocoon easily. To accurately evaluate the toxicity of CAP to silkworms and clarify the mechanism of its effect on silk gland function, we proposed a novel toxicity evaluation method based on the body weight changes after CAP exposure. We also analyzed the Ca2+-related ATPase activity, characterized energy metabolism and transcriptional changes about the autophagy key genes on the downstream signaling pathways. The results showed that after a low concentration of CAP exposed for 96 h, there were CAP residues in the silk glands of B. mori, the activities of Ca2+-ATPase and Ca2+-Mg2+-ATPase decreased significantly (P ≤ 0.01), and the activation of AMPK-related genes AMPK-α and AMPK-ß were up-regulated by 6.39 ± 0.02-fold and 12.33 ± 1.06-fold, respectively, reaching a significant level (P ≤ 0.01)). In addition, the autophagy-related genes Atg1, Atg6, Atg5, Atg7, and Atg8 downstream AMPK were significantly up-regulated at 96 h (P ≤ 0.05). The results of immunohistochemistry and protein expression assay for autophagy marker Atg8 further confirmed the occurrence of autophagy. Overall, our results indicate that CAP exposure leads to autophagy in the silk gland of B. mori and affects their physiological functions, which provides guidance for the evaluation of toxicity of low concentration environmental CAP residues to insects.


Assuntos
Bombyx , Animais , Proteínas Quinases Ativadas por AMP/genética , Autofagia , Adenosina Trifosfatases , Seda
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