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1.
Chem Biol Interact ; 311: 108795, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31419397

RESUMO

Citreoviridin (CIT), a mycotoxin and ATP synthase inhibitor, is regarded as one of aetiology factors of cardiac beriberi and Keshan disease. Thiamine (VB1) and selenium (Se) improve the recovery of these two diseases respectively. The underlying mechanisms of cardiotoxic effect of CIT and cardioprotective effect of VB1 and Se have not been fully elucidated. In this study, we found that ectopic ATP synthase was more sensitive to CIT treatment than mitochondrial ATP synthase in H9c2 cardiomyocytes. CIT inhibited the transcriptional activity of peroxisome proliferator activated receptor gamma (PPAR-γ) in mice hearts and H9c2 cells. PPAR-γ agonist attenuated the inhibitory effect of CIT on mechanistic target of rapamycin complex 2 (mTORC2) and stimulatory effect of CIT on autophagy in cardiomyocytes. CIT induced apoptosis through lysosomal-mitochondrial axis in cardiomyocytes. PPAR-γ agonist and autophagy inhibitor alleviated CIT-induced apoptosis and accelerated cardiac biomarker. VB1 and Se accelerated the basal transcriptional activity of PPAR-γ in mice hearts and H9c2 cells. Furthermore, VB1 and Se reversed the effect of CIT on PPAR-γ, autophagy and apoptosis. Our findings defined PPAR-γ-mTORC2-autophagy pathway as the key link between CIT cardiotoxicity and cardioprotective effect of VB1 and Se. The present study would shed new light on the pathogenesis of cardiomyopathy and the cardioprotective mechanism of micronutrients.


Assuntos
Apoptose/efeitos dos fármacos , Aurovertinas/farmacologia , Autofagia/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Selênio/farmacologia , Tiamina/farmacologia , Animais , Aquaporinas/genética , Aquaporinas/metabolismo , Peso Corporal/efeitos dos fármacos , Linhagem Celular , Masculino , Alvo Mecanístico do Complexo 2 de Rapamicina/genética , Alvo Mecanístico do Complexo 2 de Rapamicina/metabolismo , Camundongos , Camundongos Endogâmicos ICR , Miocárdio/metabolismo , Miocárdio/patologia , PPAR gama/agonistas , PPAR gama/genética , PPAR gama/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Proteína X Associada a bcl-2/metabolismo
2.
Int J Nanomedicine ; 14: 4613-4624, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31308651

RESUMO

Background: Bacterial infection is a common and serious complication in orthopedic implants following traumatic injury, which is often associated with extensive soft tissue damage and contaminated wounds. Multidrug-resistant bacteria have been found in these infected wounds, especially in patients who have multi trauma and prolonged stay in intensive care units.Purpose: The objective of this study was to develop a coating on orthopedic implants that is effective against drug-resistant bacteria. Methods and results: We applied nanoparticles (30-70nm) of the trace element selenium (Se) as a coating through surface-induced nucleation-deposition on titanium implants and investigated the antimicrobial activity against drug resistant bacteria including Methicillin-resistant Staphylococcus aureus (MRSA) and Methicillin-resistant Staphylococcus epidermidis (MRSE) in vitro and in an infected femur model in rats.The nanoparticles were shown in vitro to have antimicrobial activity at concentrations as low as 0.5ppm. The nanoparticle coatings strongly inhibited biofilm formation on the implants and reduced the number of viable bacteria in the surrounding tissue following inoculation of implants with biofilm forming doses of bacteria. Conclusion: This study shows a proof of concept for a selenium nanoparticle coatings as a potential anti-infective barrier for orthopedic medical devices in the setting of contamination with multi-resistant bacteria. It also represents one of the few (if only) in vivo assessment of selenium nanoparticle coatings on reducing antibiotic-resistant orthopedic implant infections.


Assuntos
Anti-Infecciosos/farmacologia , Materiais Revestidos Biocompatíveis/farmacologia , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Nanopartículas/química , Ortopedia , Próteses e Implantes , Selênio/farmacologia , Staphylococcus epidermidis/efeitos dos fármacos , Animais , Biofilmes/efeitos dos fármacos , Placas Ósseas , Parafusos Ósseos , Células Cultivadas , Contagem de Colônia Microbiana , Humanos , Masculino , Nanopartículas/ultraestrutura , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Ratos Sprague-Dawley , Titânio/farmacologia
3.
Food Chem ; 300: 125202, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31330374

RESUMO

The aim of a three-year study was to assess the effect of combined biofortification with I and Se in carrot. Four cultivars ('Askona' F1, 'Samba' F1, 'Kazan' F1 and 'White Satin') were grown in soil fertilized with KI (4 kg I ha-1) and Na2SeO4 (0.25 kg Se ha-1). The I + Se fertilization did not affect yield but the plants of all cultivars accumulated both elements in leaves and roots. On average, the I and Se contents in roots increased 7.7-times for I and 4.9-times for Se as well as the average I:Se molar ratio was 0.28:1. The contents of both elements in roots remained well below the hazard threshold thus the intake of 100 g of biofortified carrot would substantially cover the RDA for I and Se. The changes in chemical composition of roots (nitrates, phenolic compounds, sugars, carotenoids, macro-, microelements and cadmium) were rather year-dependent than affected by the applied I + Se fertilization.


Assuntos
Biofortificação/métodos , Daucus carota/química , Iodo/farmacologia , Raízes de Plantas/química , Selênio/farmacologia , Cádmio/análise , Carotenoides/análise , Daucus carota/efeitos dos fármacos , Daucus carota/crescimento & desenvolvimento , Fertilizantes , Alimentos Fortificados/análise , Iodo/análise , Iodo/farmacocinética , Polônia , Selênio/análise , Selênio/farmacocinética , Solo/química
4.
Bratisl Lek Listy ; 120(6): 449-455, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31223026

RESUMO

OBJECTIVE: The effects of quercetin and selenium on oxidative stress in endometrial adenocarcinoma cells are unclear. In this study, the effects of quercetin and selenium on oxidative stress caused by both hydrogen peroxide and UV radiation in endometrial adenocarcinoma cells were examined. METHODS: The viability of endometrial adenocarcinoma cells cultured in vitro and treated with different concentrations of quercetin and sodium selenite was measured using the MTT assay. Malondialdehyde (MDA) levels were investigated, and expression levels of BAD and p53 genes were analysed using real­time quantitative polymerase chain reaction. Acridine orange/ethidium bromide staining technique was applied to detect apoptosis. Mass attenuation coefficient of each quercetin and sodium selenite combinations was evaluated using Monte Carlo simulation. RESULTS: The combination of quercetin and sodium selenite enhanced cell viability, and reduced MDA levels. The expression levels of BAD and p53 genes decreased by combined treatment with quercetin and selenium while showing synergistic effects in terms of gene expression. Fluorescent microscopic examination showed a decrease in apoptotic cells in endometrial adenocarcinoma cells treated with the combination of quercetin and selenium. CONCLUSIONS: For the first time, selenium and quercetin have synergistic cytoprotective and radioprotective effects on oxidative stress caused by hydrogen peroxide in endometrial adenocarcinoma cells for the first time (Tab. 1, Fig. 7, Ref. 39).


Assuntos
Adenocarcinoma , Neoplasias do Endométrio , Estresse Oxidativo , Quercetina , Selênio , Adenocarcinoma/patologia , Apoptose , Neoplasias do Endométrio/patologia , Feminino , Humanos , Peróxido de Hidrogênio , Malondialdeído , Estresse Oxidativo/efeitos dos fármacos , Quercetina/farmacologia , Selênio/farmacologia
5.
Int J Nanomedicine ; 14: 3845-3860, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31213805

RESUMO

Background: Delay or failure of bone union is a significant clinical challenge all over the world, and it has been reported that bone marrow mesenchymal stem cells (BMSCs) offer a promising approach to accelerate bone fracture healing. Se can modulate the proliferation and differentiation of BMSCs. Se-treatment enhances the osteoblastic differentiation of BMSCs and inhibiting the differentiation and formation of mature osteoclasts. The purpose of this study was to assess the effects of porous Se@SiO2 nanocomposite on bone regeneration and the underlying biological mechanisms. Methods: We oxidized Se2- to develop Se quantum dots, then we used the Se quantum dots to form a solid Se@SiO2 nanocomposite which was then coated with polyvinylpyrrolidone (PVP) and etched in hot water to synthesize porous Se@SiO2 nanocomposite. We used XRD pattern to assess the phase structure of the solid Se@SiO2 nanocomposite. The morphology of porous Se@SiO2 nanocomposite were evaluated by scanning electron microscope (SEM) and the biocompatibility of porous Se@SiO2 nanocomposite were investigated by cell counting kit-8 (CCK-8) assays. Then, a release assay was also performed. We used a Transwell assay to determine cell mobility in response to the porous Se@SiO2 nanocomposite. For in vitro experiments, BMSCs were divided into four groups to detect reactive oxygen species (ROS) generation, cell apoptosis, alkaline phosphatase activity, calcium deposition, gene activation and protein expression. For in vivo experiments, femur fracture model of rats was constructed to assess the osteogenic effects of porous Se@SiO2 nanocomposite. Results: In vitro, intervention with porous Se@SiO2 nanocomposite can promote migration and osteogenic differentiation of BMSCs, and protect BMSCs against H2O2-induced inhibition of osteogenic differentiation. In vivo, we demonstrated that the porous Se@SiO2 nanocomposite accelerated bone fracture healing using a rat femur fracture model. Conclusion: Porous Se@SiO2 nanocomposite promotes migration and osteogenesis differentiation of rat BMSCs and accelerates bone fracture healing, and porous Se@SiO2 nanocomposite may provide clinic benefit for bone tissue engineering.


Assuntos
Células da Medula Óssea/citologia , Diferenciação Celular/efeitos dos fármacos , Fraturas do Fêmur/terapia , Consolidação da Fratura/efeitos dos fármacos , Células-Tronco Mesenquimais/citologia , Nanocompostos/química , Osteogênese/efeitos dos fármacos , Selênio/farmacologia , Dióxido de Silício/farmacologia , Animais , Apoptose/efeitos dos fármacos , Células Cultivadas , Citoproteção/efeitos dos fármacos , Modelos Animais de Doenças , Fraturas do Fêmur/diagnóstico por imagem , Fraturas do Fêmur/patologia , Peróxido de Hidrogênio/toxicidade , Nanocompostos/ultraestrutura , Porosidade , Ratos Sprague-Dawley , Transdução de Sinais , Microtomografia por Raio-X
6.
Life Sci ; 231: 116578, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31211996

RESUMO

AIMS: The aim of the present study is to shed light on the modulating action of selenium on two of the most crucial cellular pathways; apoptosis and autophagy and the possible interplay between them in determining the pituitary fate in the context of mercury intoxication through demonstration of the molecular, histopathological, immunohistochemical, and ultrastructural features of selenium mercury-treated adenohypophysis. METHODS: Thirty adult Sprague Dawley male albino rats were assigned into control group, mercury-treated group and mercury­selenium concomitantly-treated group. The adenohypophysis was subjected to structural, molecular and protein expression assessment of autophagy and apoptotic markers and western blotted analysis of Beclin 1 as a key cross-regulator of autophagy and apoptosis. KEY FINDINGS: Selenium treatment ameliorated the mercury-induced apoptosis detected by improvement in PCR and immunohistochemical expression of the apoptotic markers Bax, Bcl-2 and Caspase-3. Selenium also improved mercury-induced autophagic dysfunction with statistically significant improvement in western blotted levels of the autophagy markers LC3I, LC3II and Beclin1. The histopathological and ultrastructural studies strongly confirmed those findings. SIGNIFICANCE: The crosstalk between the apoptotic Bcl-2 family of proteins and the autophagic Beclin-1LC3 pathway in the context of mercury intoxication paves the way for developing novel effective treatment strategies for several mercury-induced pituitary diseases.


Assuntos
Intoxicação por Mercúrio/prevenção & controle , Mercúrio/toxicidade , Hipófise/efeitos dos fármacos , Selênio/farmacologia , Animais , Apoptose/efeitos dos fármacos , Proteínas Reguladoras de Apoptose/metabolismo , Autofagia/efeitos dos fármacos , Proteína Beclina-1/metabolismo , Caspase 3/metabolismo , Masculino , Intoxicação por Mercúrio/metabolismo , Intoxicação por Mercúrio/patologia , Hipófise/metabolismo , Hipófise/patologia , Adeno-Hipófise/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Ratos Sprague-Dawley , Proteína X Associada a bcl-2/metabolismo
7.
Chem Commun (Camb) ; 55(49): 7037-7040, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31143887

RESUMO

A π-extended red absorbing Se-rhodamine (Se-NR) was synthesised and characterized. By masking the amine of Se-NR as an azide, we successfully constructed a bio-orthogonally activatable photosensitizer (Se-NR-Az). Se-NR-Az was not photocytotoxic, but when activated by the Staudinger reaction, photocytotoxicity was restored.


Assuntos
Azidas/farmacologia , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Rodaminas/farmacologia , Selênio/farmacologia , Azidas/química , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Células HeLa , Humanos , Estrutura Molecular , Fármacos Fotossensibilizantes/síntese química , Fármacos Fotossensibilizantes/química , Rodaminas/química , Selênio/química , Relação Estrutura-Atividade
8.
Oxid Med Cell Longev ; 2019: 1503478, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31049125

RESUMO

When dairy cows are exposed to high-temperature environment, their antioxidant capacity and productive performance decrease, leading to economic losses. Emerging evidence has shown that selenium (Se) can effectively alleviate heat stress in dairy cows; however, the cellular mechanism underlying this protection is not clear. The purpose of this study was to investigate and compare the protective effects of inorganic Se (sodium selenite, SS) and organic Se (selenite methionine, SM) in MAC-T (mammary alveolar cells-large T antigen, a bovine mammary epithelial cell (BMEC) line) cells during heat stress. MAC-T cells were treated in 4 ways unless otherwise described: (i) cells in the heat treatment (HT) group were cultured at 42.5°C for 1 h and then recovered in 37°C for another 12 h; (ii) the SM group was pretreated with organic Se for 2 h, cultured at 42.5°C for 1 h, and then recovered in 37°C for 12 h; (iii) the SS group was treated similarly to the SM group except that the cells were pretreated with inorganic Se instead of organic Se; and (iv) the control group was continuously cultured in 37°C and received no Se treatment. The results showed that heat shock at 42.5°C for 1 h triggered heat shock response, sabotaged the redox balance, and reduced cell viability in MAC-T cells; and pretreatment of cells with SM or SS effectively alleviated the negative effects of heat shock on the cells. However, the cells were much more sensitive to SS treatment but more tolerant to SM. In addition, two forms of Se appeared to affect the expression of different genes, including nuclear factor erythroid 2-related factor 2 (Nrf2) and inducible nitric oxide synthase (iNOS) in the SM group and thioredoxin reductase 1 (TXNRD1) in the SS group in Nrf2-ARE (antioxidant response element) antioxidant pathway and inflammation response. In summary, results showed the mechanistic differences in the protective effects of organic and inorganic Se on heat stress in BMECs.


Assuntos
Antioxidantes/farmacologia , Células Epiteliais/metabolismo , Resposta ao Choque Térmico/efeitos dos fármacos , Glândulas Mamárias Animais/metabolismo , Selênio/farmacologia , Selenito de Sódio/farmacologia , Animais , Bovinos , Linhagem Celular , Células Epiteliais/patologia , Feminino , Glândulas Mamárias Animais/patologia , Fator 2 Relacionado a NF-E2/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Tiorredoxina Redutase 1/metabolismo
9.
Ecotoxicol Environ Saf ; 180: 179-184, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31082582

RESUMO

Selenium (Se) and zinc (Zn) are necessary mineral nutrients for human body but millions of people have an inadequate intake of them, and eat food enriched with Se and Zn may minimize these problems. Chinese cabbage is an important food in people's daily life. The aim of this study was to evaluate the effects of single Se, Zn and their combination treatment in soil on their accumulation, antioxidant system and lipid peroxidation in roots and leaves of Chinese cabbage using soil pot culture experiment. When 0.5 mg kg-1 Se +30 mg kg-1 Zn and 1.0 mg kg-1 Se +30 mg kg-1 Zn were spiked in soils, Zn concentrations in roots and leaves of Chinese cabbage were significantly increased (p < 0.05) by 20.2%, 37.8% and 17.9%, 34.1% respectively compared to the treatment of 30 mg kg-1 Zn added, and the latter was significantly higher (p < 0.05) than that of former, indicating Se significantly promoted Zn accumulation. Almost all physiological indexes including POD, SOD, CAT, APX, GR, Chlorophyll a, Chlorophyll b, Carotenoids, MDA and Free proline in the treatments of Se or Zn spiked were significantly improved (p < 0.05) or basically unaffected compared to the control without Se or Zn added. The biomass change trends were similar with these indexes either. These results showed that the addition in soil of Se and Zn significantly increased their accumulation in Chinese cabbage without affected its formal growth. Particularly, the addition of Se promoted Zn accumulation. The conclusions were more important reference for the production practice of cash crop enriched of Se and Zn either.


Assuntos
Brassica/efeitos dos fármacos , Selênio/farmacologia , Solo , Zinco/metabolismo , Antioxidantes/metabolismo , Brassica/enzimologia , Brassica/metabolismo , Carotenoides/metabolismo , Clorofila/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/enzimologia , Folhas de Planta/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/enzimologia , Raízes de Plantas/metabolismo , Selênio/metabolismo
10.
Microbiol Immunol ; 63(7): 269-279, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31141221

RESUMO

Pseudorabies, a herpesvirus infection, is mainly controlled by using attenuated live vaccines. In this study, the effect of ginseng stem and leaf saponins (GSLS) in combination with selenium (Se; in the form of sodium selenite) on vaccination against attenuated pseudorabies virus (aPrV) was evaluated. It was found that GSLS and Se have an adjuvant effect and that a combination of GSLS and Se stimulates significantly enhanced immune responses than does GSLS or Se alone. Following oral administration of GSLS, mice immunized with an attenuated PrV vaccine diluted in Se-containing physiological saline solution (PSS) provoked a significantly stronger gB-specific serum antibodies response (IgG, IgG1 and IgG2a), enhanced lymphocyte proliferation and cytolytic activity of NK cells, along with higher production of cytokines (IFN-γ, IL-12, IL-5 and IL-10) by splenocytes. Notably, the combination of GSLS and Se conferred a much higher resistance to fPrV challenge after immunization of the mice with aPrV vaccine. This study offers convincing experimental evidence that an injection of Se with oral GSLS is a promising adjuvant combination that improves the efficacy of vaccination against PrV and deserves further study regarding improvement of responses to other animal vaccines.


Assuntos
Imunidade Adaptativa/efeitos dos fármacos , Herpesvirus Suídeo 1/imunologia , Panax/química , Folhas de Planta/química , Vacinas contra Pseudorraiva/imunologia , Saponinas/farmacologia , Selênio/farmacologia , Vacinas Atenuadas/imunologia , Adjuvantes Imunológicos/administração & dosagem , Administração Oral , Animais , Formação de Anticorpos , Proliferação de Células/efeitos dos fármacos , Citocinas/metabolismo , Combinação de Medicamentos , Feminino , Febre Aftosa/prevenção & controle , Imunização , Imunoglobulina G/sangue , Células Matadoras Naturais/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos ICR , Doença de Newcastle/prevenção & controle , Extratos Vegetais/farmacocinética , Pseudorraiva/prevenção & controle , Saponinas/administração & dosagem , Selênio/administração & dosagem , Vacinação , Vacinas Atenuadas/administração & dosagem
11.
Ecotoxicol Environ Saf ; 180: 588-599, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31132554

RESUMO

Past studies have already determined that selenium (Se) is very effective in alleviating cell oxidative damage caused by various abiotic stresses in plants. Past studies have also indicated other physiological pathways by which Se may benefit plants. In order to better understand the full array of potential applications for Se in agriculture, this study investigated the influence of Se on carbohydrate and nitrogen (N) metabolism in potato plants (Solanum tuberosum L. cv. Sante) grown under cadmium (Cd) and/or arsenic (As) toxicity. Potato plants were grown in a growth chamber and fertigated with Hoagland nutrient solution with or without Se (9 µM). After 48-d of growth under Cd (40 µM) and/or As (40 µM) stress, carbohydrate and N metabolism in leaves, roots and stolons were measured. For carbohydrate metabolism, various sugars-i.e., sucrose, starch, glucose, fructose, and total soluble sugar contents (TSSC)-and the activities of enzymes associated with sucrose metabolism and glycolysis-i.e., acid invertase (AI), neutral invertase (NI), sucrose-synthetase (SS), sucrose phosphatesynthetase (SPS), fructokinase (FK), hexokinase (HK), phosphofructokinase (PFK), and pyruvatekinase (PK)-were measured. For N metabolism, NO3-, NO2- and NH4+ contents along with the enzymatic activities of nitrate reductase (NRA), nitrite reductase (NiRA), glutamine-synthetase (GS), and glutamate-synthetase (GOGAT) were measured. Overall, Cd and/or As treatments had reduced plant growth relative to those plants grown without heavy metal toxicity, due to hindered photosynthesis and alterations in N metabolism and glycolysis. Regarding N metabolism, heavy metal toxicity caused a reduction in NO3- and NO2- content and NRA and NiRA enzymatic activity and enhanced NH4+ content and GDH activity in leaves, roots and stolons. Regarding glycolysis, the activity of enzymes of glycolysis-i.e., FK, HK, PFK, and PK-were also reduced. In the C metabolism study, plants combatted Cd and As toxicity naturally by an adaptation mechanism which caused an increase in soluble sugars (fructose, glucose, sucrose) by increasing NI, SS and SSP enzymatic activity. Supplementation with Se in the Cd and/or As treatments in the carbohydrate and N metabolism studies improved plant growth. Selenium supplementation in the Cd and As treatments decreased Cd and/or As content in the plant tissue and alleviating the Cd- and/or As-induced toxicity by enhancing the C-metabolism adaptation mechanism. Applying Se to Cd and As treatments also decreased nitrogen losses by hindering Cd- and As-induced changes in the N-metabolism. Se also limited Cd and As accumulation in the plant tissue by the antagonistic effect between Cd/Se and As/Se in the roots. The results of this study indicate that in the presence of Cd and/or As. soil toxicity, Se may be a powerful tool for promoting plant growth.


Assuntos
Arsênico/toxicidade , Cádmio/toxicidade , Metabolismo dos Carboidratos/efeitos dos fármacos , Nitrogênio/metabolismo , Selênio/farmacologia , Solanum tuberosum/efeitos dos fármacos , Fotossíntese/efeitos dos fármacos , Solanum tuberosum/metabolismo , Estresse Fisiológico , Sacarose/metabolismo
12.
Environ Toxicol ; 34(8): 968-978, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31077554

RESUMO

The aim of this study was to investigate the protective effects of Nano-Se against Ni-induced testosterone synthesis disorder in rats and determine the underlying protective mechanism. Sprague-Dawley rats were co-treated with Ni (5.0 mg/kg, i.p.) and Nano-Se (0.5, 1.0, and 2.0 mg/kg, oral gavage) for 14 days after which various endpoints were evaluated. The Ni-induced abnormal pathological changes and elevated 8-OHdG levels in the testes were attenuated by Nano-Se administration. Importantly, decreased serum testosterone levels in the Ni-treated rats were significantly restored by Nano-Se treatment, particularly at 1.0 and 2.0 mg/kg. Furthermore, the mRNA and protein levels of testosterone synthetase were increased by Nano-Se compared to the Ni group, whereas phosphorylated protein expression levels of mitogen-activated protein kinase (MAPK) pathways were suppressed by Nano-Se administration in the Ni-treated rats. Overall, the results suggest that Nano-Se may ameliorate the Ni-induced testosterone synthesis disturbance via the inhibition of ERK1/2, p38, and JNK MAPK pathways.


Assuntos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Níquel/toxicidade , Selênio/farmacologia , Testosterona/biossíntese , Animais , MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Proteínas Quinases JNK Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Masculino , Nanopartículas , Fosforilação/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Testículo/efeitos dos fármacos , Testículo/metabolismo , Testículo/patologia , Testosterona/sangue , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
13.
Environ Sci Pollut Res Int ; 26(21): 21828-21834, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31134545

RESUMO

The effect of selenium on excessive fatty acid-induced apoptosis and abnormal amino acid metabolism in the liver is well known, because it is an important site in the fatty acid metabolism pathway. However, the protective role of nano-elemental selenium (nano-Se) supplementation against hexavalent chromium (K2Cr2O7)-induced abnormal fatty acid metabolism has not been evaluated yet. Therefore, we conducted chicken experiments with different nano-Se supplementation doses to investigate the role of nano-Se against Cr(VI)-induced adverse effects. For this purpose, a total of 120 1-day-old chicks were randomly divided into control group, Cr(VI)-exposed group, protection group, treatment group, prevention group, and nano-Se control group. The results of RT-qPCR showed that the nano-Se supplementation notably downregulated (P < 0.01) the messenger RNA (mRNA) expression levels of fatty acid synthase (FASN), whereas nano-Se supplementation significantly upregulated (P < 0.01) the mRNA expression level of acyl-coenzyme A oxidase 1 (ACOX1) in chicken's liver at day 35 of the experiment. Similar results were further verified by western blot analysis. Moreover, nano-Se supplementation significantly enhanced and reduced the antibody expression levels of ACOX1 and FASN in immunohistochemical analysis, respectively. Thus, finally, it was concluded that nano-Se can alleviate K2Cr2O7-induced abnormal fatty acid metabolism in chicken's liver.


Assuntos
Galinhas/metabolismo , Cromo/toxicidade , Ácidos Graxos/metabolismo , Fígado/efeitos dos fármacos , Selênio/farmacologia , Acil-CoA Oxidase/genética , Acil-CoA Oxidase/metabolismo , Animais , Apoptose/efeitos dos fármacos , Suplementos Nutricionais , Ácido Graxo Sintase Tipo I/genética , Ácido Graxo Sintase Tipo I/metabolismo , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos , Metabolismo dos Lipídeos/genética , Fígado/metabolismo , Nanopartículas/administração & dosagem , Nanopartículas/química , Substâncias Protetoras/farmacologia , Selênio/administração & dosagem , Selênio/química
14.
IET Nanobiotechnol ; 13(1): 30-35, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30964034

RESUMO

This study was designed to evaluate and compare the stability, antioxidant and anticancer activities of selenium nanoparticles (SeNPs) decorated with different molecular weight (MW) of chitosan (CS) (1500 Da, 48 kDa, 510 kDa). The size range of well-dispersed SeNPs was effectively controlled by I- first and then coated with CS. The morphology, size and surface charge of generated SeNPs were characterised by several technologies. Fourier transform infrared spectroscopy was used to investigate the relationship between SeNPs and CS. SeNPs decorated with CS (510 kDa) can keep stable for more than 45 days. As observed from the results of a simple photometric system, the antioxidant activities of decorated SeNPs were enhanced compared to undecorated SeNPs. SeNPs coated with higher MW of CS (510 kDa) showed the strongest antioxidant activities. Moreover, the treatments of SeNPs decorated with CS inhibited the growth of HepG2 cells in a time- and dose-dependent manner. The proposed results demonstrated the critical roles of the MW of CS on the stability, antioxidant and anticancer properties of CS-coated SeNPs, which provided an important design cue for future applications of functional foods and additives.


Assuntos
Antineoplásicos , Antioxidantes , Quitosana , Nanopartículas/química , Selênio , Antineoplásicos/química , Antineoplásicos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Quitosana/química , Quitosana/farmacologia , Células Hep G2 , Humanos , Peso Molecular , Selênio/química , Selênio/farmacologia
15.
J Anim Sci ; 97(6): 2569-2582, 2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-30957833

RESUMO

The goal of this study was to test the hypothesis that sodium selenite (ISe), SEL-PLEX (OSe), vs. a 1:1 blend (MIX) of ISe and OSe in a basal vitamin-mineral mix would differentially affect serological and hepatic parameters of growing steers grazing toxic endophyte-infected tall fescue-mixed forage pasture. Predominately Angus steers (BW = 183 ± 34 kg) were randomly selected from herds of fall-calving cows grazing endophyte-infected tall fescue-mixed pasture and consuming vitamin-mineral mixes that contained 35 ppm Se as ISe, OSe, and MIX forms. Steers were weaned, depleted of Se for 98 d, and subjected to summer-long common grazing of an endophyte-infected tall fescue-mixed pasture (0.51 ppm total ergovaline + ergovalinine; 10.1 ha). Steers were assigned (n = 8 per treatment) to the same Se form treatments upon which they were raised. Se treatments were administered by daily top-dressing 85 g of vitamin-mineral mix onto 0.23 kg soyhulls, using in-pasture Calan gates. The PROC MIXED procedure of SAS was used to assess the effect of Se form treatments on serum parameters at day 0, 22, 43, 64, and 86. After slaughter, the effect of Se treatment on hepatic alkaline phosphatase (tissue nonspecific isoform, TNALP) mRNA, protein, and albumin protein content was assessed using the PROC GLM procedure of SAS. Fisher's protected LSD procedure was used to separate treatment means. Partial correlation analysis was used to evaluate the relationship among whole blood Se concentration and serum parameters, accounting for the effect of time. Across periods, MIX steers had more (P ≤ 0.04) serum albumin than OSe and ISe steers, respectively. However, the relative hepatic bovine serum albumin protein content was not affected (P = 0.28) by Se treatments. Serum alkaline phosphatase activity was greater (P ≤ 0.01) in MIX and OSe steers. Similarly, hepatic TNALP protein content in MIX steers was greater (P = 0.01) than ISe steers. Partial correlation analysis revealed that serum albumin, blood urea nitrogen, and alkaline phosphatase activity were correlated (r ≥ 0.23, P ≤ 0.02) with whole blood Se concentration. In summary, consumption of 3 mg Se/d as OSe or MIX forms of Se in vitamin-mineral mixes increased serum albumin concentration and alkaline phosphatase activity, the reduction of which is associated with fescue toxicosis. We conclude that the organic forms of Se ameliorated the depression of 2 of known serological biomarkers of fescue toxicosis.


Assuntos
Ração Animal/análise , Doenças dos Bovinos/prevenção & controle , Bovinos/fisiologia , Endófitos/fisiologia , Festuca/microbiologia , Selênio/química , Fosfatase Alcalina/efeitos dos fármacos , Fosfatase Alcalina/metabolismo , Animais , Nitrogênio da Ureia Sanguínea , Fígado/metabolismo , Masculino , Minerais/química , Distribuição Aleatória , Estações do Ano , Selênio/farmacologia , Albumina Sérica/efeitos dos fármacos , Selenito de Sódio/química , Selenito de Sódio/farmacologia , Ureia/metabolismo , Vitaminas/química , Vitaminas/metabolismo
16.
In Vivo ; 33(3): 763-769, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31028195

RESUMO

BACKGROUND/AIM: Alcoholic liver disease is an important health problem which is reversible during early stages of liver damage, but becomes permanent with time. Nowadays, many studies focus on various agents that prevent, reduce or slow the progression of the toxic effects of alcohol. In our study, we investigated the efficiency of ozone and selenium against oxidative damage in a model of alcohol-induced liver damage. MATERIALS AND METHODS: Forty-eight female Wistar Albino rats between 4 and 6 months of age and weighing 190-250 g were included in the study and were used as models of alcohol liver damage. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum and tissue total oxidant levels, serum and tissue total antioxidant levels, and the histopathological evaluation of the liver were performed in 8 groups. RESULTS: In the statistical analysis, it was observed that ozone and/or selenium therapies decreased the AST levels. Total oxidant and antioxidant serum levels were found to vary in serum and tissue. Ozone and/or selenium therapies decreased liver damage, according to histopathological findings. CONCLUSION: Through ozone and/or selenium therapies, less damage was observed histopathologically compared to the alcohol group. It is thought that the results of our study can be used in individual treatments following confirmation of liver damage in alcoholic patients.


Assuntos
Antioxidantes/farmacologia , Hepatopatias Alcoólicas/metabolismo , Hepatopatias Alcoólicas/patologia , Oxidantes Fotoquímicos/farmacologia , Ozônio/farmacologia , Selênio/farmacologia , Animais , Biomarcadores , Modelos Animais de Doenças , Feminino , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Hepatócitos/patologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Hepatopatias Alcoólicas/tratamento farmacológico , Ratos , Resultado do Tratamento
17.
Int J Mol Sci ; 20(8)2019 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-31010052

RESUMO

Early blight is a disease that greatly affects Solanaceae, mainly damaging tomato plants, and causing significant economic losses. Although there are methods of biological control, these are very expensive and often their mode of action is slow. Due to this, there is a need to use new techniques that allow a more efficient control of pathogens. Nanotechnology is a new alternative to solve these problems, allowing the creation of new tools for the treatment of diseases in plants, as well as the control of pathogens. The aim of the present investigation was to evaluate the foliar application of selenium and copper in the form of nanoparticles in a tomato crop infested by Alternaria solani. The severity of Alternaria solani, agronomic variables of the tomato crop, and the changes in the enzymatic and non-enzymatic antioxidant compounds were evaluated. The joint application of Se and Cu nanoparticles decreases the severity of this pathogen in tomato plants. Moreover, high doses generated an induction of the activity of the enzymes superoxide dismutase, ascorbate peroxidase, glutathione peroxidase (GPX) and phenylalanine ammonia lyase in the leaves, and the enzyme GPX in the fruit. Regarding non-enzymatic compounds in the leaves, chlorophyll a, b, and totals were increased, whereas vitamin C, glutathione, phenols, and flavonoids were increased in fruits. The application of nanoparticles generated beneficial effects by increasing the enzymatic and non-enzymatic compounds and decreasing the severity of Alternaria solani in tomato plants.


Assuntos
Alternaria/fisiologia , Cobre/farmacologia , Lycopersicon esculentum/microbiologia , Lycopersicon esculentum/fisiologia , Nanopartículas/química , Selênio/farmacologia , Estresse Fisiológico/efeitos dos fármacos , Alternaria/efeitos dos fármacos , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Produtos Agrícolas/efeitos dos fármacos , Produtos Agrícolas/crescimento & desenvolvimento , Lycopersicon esculentum/efeitos dos fármacos , Lycopersicon esculentum/crescimento & desenvolvimento , Fenilalanina Amônia-Liase/metabolismo , Pigmentos Biológicos/metabolismo , Doenças das Plantas/microbiologia , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/metabolismo
18.
Theriogenology ; 131: 146-152, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30965207

RESUMO

Lead (Pb) is an environmental pollutant and has toxic effect on birds. Selenium (Se) has alleviative effect on Pb poisoning. This study investigated mitigative effect of Se on autophagy in Pb-treated chicken testes. Seven-day-old male chickens were randomly divided into four groups with 45 birds in each group. The birds of the control group were offered drinking water (DW) and commercial diet (CD) (0.49 mg/kg Se). The birds of the Se group were offered DW and CD containing sodium selenite (SeCD) (1.00 mg/kg Se). The birds of the Pb group were offered DW containing lead acetate (PbDW) (350.00 mg/L Pb) and CD. The birds of the Pb/Se group were offered PbDW and SeCD. On the 30th, 60th, and 90th days, respectively; histology, antioxidant indexes (hydrogen peroxide (H2O2), catalase (CAT), total antioxidant capacity (TAOC), reduced glutathione (GSH), and superoxide dismutase (SOD)), mRNA and protein levels of autophagy-related genes (autophagy-related proteins 5, Beclin 1, Dynein, light chain 3 (LC3)-I, LC3-II, and mammalian target of rapamycin (mTOR)) were performed in chicken testes. The results of this study showed that Pb caused histological changes; increased H2O2 content; decreased CAT, TAOC, and SOD activities and GSH content; and increased mRNA and protein levels of the above autophagy-related genes except that mTOR decreased in chicken testes. Se alleviated the above changes. Se alleviated histological damage, oxidative stress, and autophagy in the Pb-treated chicken testes.


Assuntos
Autofagia/efeitos dos fármacos , Galinhas , Poluentes Ambientais/toxicidade , Chumbo/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Selênio/farmacologia , Testículo/efeitos dos fármacos , Animais , Proteínas Aviárias/genética , Proteínas Aviárias/metabolismo , Biomarcadores/metabolismo , Masculino , RNA Mensageiro/metabolismo , Distribuição Aleatória
19.
Biomolecules ; 9(4)2019 03 30.
Artigo em Inglês | MEDLINE | ID: mdl-30935009

RESUMO

This work was designated to scrutinize the protein differential expression in natural selenium-enriched and non-selenium-enriched rice using the Isobaric-tags for relative and absolute quantification (iTRAQ) proteomics approach. The extracted proteins were subjected to enzyme digestion, desalting, and identified by iTRAQ coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology. High pH C18 separation analysis was performed, and the data were then analyzed by Protein PilotTM (V4.5) search engine. Protein differential expression was searched out by comparing relatively quantified proteins. The analysis was conducted using gene ontology (GO), cluster of orthologous groups of proteins (COG) and Kyoto encyclopedia of genes and genomes (KEGG) metabolic pathways. A total of 3235 proteins were detected and 3161 proteins were quantified, of which 401 were differential proteins. 208 down-regulated and 193 up-regulated proteins were unveiled. 77 targeted significant differentially expressed proteins were screened out for further analysis, and were classified into 10 categories: oxidoreductases, transferases, isomerases, heat shock proteins, lyases, hydrolases, ligases, synthetases, tubulin, and actin. The results indicated that the anti-stress, anti-oxidation, active oxygen metabolism, carbohydrate and amino acid metabolism of natural selenium-enriched rice was higher than that of non-selenium rice. The activation of the starch synthesis pathway was found to be bounteous in non-selenium-enriched rice. Cysteine synthase (CYS) and methyltransferase (metE) might be the two key proteins that cause amino acid differences. OsAPx02, CatC, riPHGPX, HSP70 and HSP90 might be the key enzymes regulating antioxidant and anti-stress effect differences in two types of rice. This study provides basic information about deviations in protein mechanism and secondary metabolites in selenium-enriched and non-selenium-enriched rice.


Assuntos
Oryza/efeitos dos fármacos , Proteínas de Plantas/genética , Proteômica , Selênio/farmacologia , Cromatografia Líquida , Biologia Computacional , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/genética , Oryza/metabolismo , Proteínas de Plantas/análise , Proteínas de Plantas/metabolismo , Espectrometria de Massas em Tandem
20.
Environ Sci Pollut Res Int ; 26(16): 16220-16228, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30972675

RESUMO

Cadmium (Cd) is absorbed readily by rice plants and is transferred to humans when contaminated rice is consumed. Adding selenium (Se) to the plant nutrient solutions reduces the accumulation of Cd in the rice (Oryza sativa L.) seedlings. However, as the relevant underlying mechanism remains unclear, the aim of our study was to improve our understanding of the Se-mediated resistance to Cd stress in rice. We conducted hydroponic experiments to study the effects of selenite or selenate on Cd subcellular distribution and xylem transport in rice seedlings under Cd stress, and we investigated the antioxidative defense responses in the rice plants. We found that the supplementation of both Se forms decreased the Cd accumulations in the roots and shoots of the rice plants. The selenite addition significantly decreased the Cd contents in different subcellular fractions of the rice roots, increased the proportion of Cd distributed to soluble cytosol by 23.41%, and decreased the Cd distribution in the organelle by 28.74% in contrast with the treatment with Cd only. As regards the selenate addition, only the Cd distribution ratio of cytosol was increased by 13.07%. After adding selenite, a decrease of 55.86% in the Cd concentration in xylem sap was observed, whereas little change was found after treatment co-applied with selenate. The hydrogen peroxide (H2O2) and malondialdehyde(MDA) contents in the rice roots were elevated under Cd stress, and the addition of selenite and selenate decreased the H2O2 levels by 77.78% and 59.26%, respectively. Co-exposure to Cd and Se elevated the glutathione (GSH) accumulations in the rice shoots and roots, with the degree of increase being the following: co-applied with selenite > co-applied with selenate > Cd alone treatment. Exposure to Cd increased the catalase (CAT) activity in the roots significantly, whereas it decreased in the shoots. After selenite or selenate supplementation, the CAT activity in the rice roots increased compared with applying only Cd. Compared with the control, the addition of Cd or Se had no significant effect on the activities of peroxidase (POD) or ascorbate peroxidase (APX). Our results showed that Se affected the Cd accumulation in rice seedlings by altering the Cd subcellular distribution and decreasing the ROS induced by Cd stress. Such effects were more significant in the selenite than in the selenate applied treatment.


Assuntos
Cádmio/farmacocinética , Oryza/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Selênio/farmacologia , Antioxidantes/metabolismo , Cádmio/toxicidade , Glutationa/metabolismo , Peróxido de Hidrogênio/metabolismo , Hidroponia , Malondialdeído/farmacologia , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/metabolismo , Plântula/efeitos dos fármacos , Plântula/metabolismo , Ácido Selênico/farmacologia , Ácido Selenioso/farmacologia , Distribuição Tecidual , Xilema/efeitos dos fármacos , Xilema/metabolismo
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