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1.
J Anim Sci ; 97(6): 2569-2582, 2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-30957833

RESUMO

The goal of this study was to test the hypothesis that sodium selenite (ISe), SEL-PLEX (OSe), vs. a 1:1 blend (MIX) of ISe and OSe in a basal vitamin-mineral mix would differentially affect serological and hepatic parameters of growing steers grazing toxic endophyte-infected tall fescue-mixed forage pasture. Predominately Angus steers (BW = 183 ± 34 kg) were randomly selected from herds of fall-calving cows grazing endophyte-infected tall fescue-mixed pasture and consuming vitamin-mineral mixes that contained 35 ppm Se as ISe, OSe, and MIX forms. Steers were weaned, depleted of Se for 98 d, and subjected to summer-long common grazing of an endophyte-infected tall fescue-mixed pasture (0.51 ppm total ergovaline + ergovalinine; 10.1 ha). Steers were assigned (n = 8 per treatment) to the same Se form treatments upon which they were raised. Se treatments were administered by daily top-dressing 85 g of vitamin-mineral mix onto 0.23 kg soyhulls, using in-pasture Calan gates. The PROC MIXED procedure of SAS was used to assess the effect of Se form treatments on serum parameters at day 0, 22, 43, 64, and 86. After slaughter, the effect of Se treatment on hepatic alkaline phosphatase (tissue nonspecific isoform, TNALP) mRNA, protein, and albumin protein content was assessed using the PROC GLM procedure of SAS. Fisher's protected LSD procedure was used to separate treatment means. Partial correlation analysis was used to evaluate the relationship among whole blood Se concentration and serum parameters, accounting for the effect of time. Across periods, MIX steers had more (P ≤ 0.04) serum albumin than OSe and ISe steers, respectively. However, the relative hepatic bovine serum albumin protein content was not affected (P = 0.28) by Se treatments. Serum alkaline phosphatase activity was greater (P ≤ 0.01) in MIX and OSe steers. Similarly, hepatic TNALP protein content in MIX steers was greater (P = 0.01) than ISe steers. Partial correlation analysis revealed that serum albumin, blood urea nitrogen, and alkaline phosphatase activity were correlated (r ≥ 0.23, P ≤ 0.02) with whole blood Se concentration. In summary, consumption of 3 mg Se/d as OSe or MIX forms of Se in vitamin-mineral mixes increased serum albumin concentration and alkaline phosphatase activity, the reduction of which is associated with fescue toxicosis. We conclude that the organic forms of Se ameliorated the depression of 2 of known serological biomarkers of fescue toxicosis.


Assuntos
Ração Animal/análise , Doenças dos Bovinos/prevenção & controle , Bovinos/fisiologia , Endófitos/fisiologia , Festuca/microbiologia , Selênio/química , Fosfatase Alcalina/efeitos dos fármacos , Fosfatase Alcalina/metabolismo , Animais , Nitrogênio da Ureia Sanguínea , Fígado/metabolismo , Masculino , Minerais/química , Distribuição Aleatória , Estações do Ano , Selênio/farmacologia , Albumina Sérica/efeitos dos fármacos , Selenito de Sódio/química , Selenito de Sódio/farmacologia , Ureia/metabolismo , Vitaminas/química , Vitaminas/metabolismo
2.
Environ Sci Pollut Res Int ; 26(15): 15685-15697, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30949948

RESUMO

Fluoride is widely distributed in the environment, and excessive fluoride intake can induce cytotoxicity, DNA damage, and cell cycle changes in many tissues and organs, including the kidney. Accumulating evidence demonstrates that selenium (Se) administration ameliorates sodium fluoride (NaF)-induced kidney damage. However, the potentially beneficial effects of Se against NaF-induced cytotoxicity of the kidney and the underlying molecular mechanisms of this protection are not fully understood. At present, in this study, the normal rat kidney cell (NRK-52E) was used to investigate the potentially protective mechanism of Se against NaF-induced apoptosis, by using the methods of pathology, colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, and Western blot. The experiment was designed with a control group, two NaF-treated groups (NaF, 5, 20 mg/L), two sodium selenite-treated groups (Na2SeO3, 17.1, 34.2 µg/L), and four Se + NaF-treated groups (Na2SeO3, 17.1, 34.2 µg/L; NaF, 5, 20 mg/L). The results indicate that selenium can attenuate apoptosis and AMPK phosphorylation in the NRK-52E cell induced with fluoride. These results imply that selenium is capable to modulate fluoride-induced NRK-52E cell apoptosis via regulating the expression levels of the proteins involved in mitochondrial pathway and changes in p-AMPK expressions may also be a key process in preventing fluorosis.


Assuntos
Apoptose/efeitos dos fármacos , Fluoretos/metabolismo , Nefropatias/fisiopatologia , Rim/efeitos dos fármacos , Selênio/metabolismo , Selenito de Sódio/metabolismo , Proteínas Quinases Ativadas por AMP , Animais , Ciclo Celular , Fluoretos/química , Nefropatias/metabolismo , Fosforilação , Ratos , Selênio/química , Selenito de Sódio/química
3.
Carbohydr Polym ; 179: 19-27, 2018 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-29111042

RESUMO

In this study, carboxylic curdlans (Cur-4, Cur-8, and Cur-24) with different molecular properties and chain conformations were used as stabilizer and capping agent to fabricate stable and water-dispersible selenium nanoparticles (SeNPs). Results showed that molecular properties and chain conformations of carboxylic curdlans remarkably influenced the size, morphology, structure, and stability of SeNPs and the carboxylic curdlan was ligated to SeNPs via OH⋯Se interaction. The as-prepared SeNPs was amorphous and showed homogeneous and monodisperse spherical structure with size of ∼50-90nm. The Cur-8-decorated SeNPs (SeNPs@Cur-8) exhibited smaller particle size (∼56nm) and greater stability than those of the others. The carboxylic curdlan-stabilized SeNPs exhibited excellent antioxidant capacities compared to the control SeNPs. Specifically, SeNPs@Cur-8 with smaller particle size possessed strong antioxidant efficacy. SeNPs@Cur-8 also exhibited low cytotoxic activity against SPCA-1 and HeLa cell lines in vitro.


Assuntos
Antioxidantes/química , Materiais Biocompatíveis/química , Nanopartículas/química , Selenito de Sódio/química , beta-Glucanas/química , Antioxidantes/síntese química , Antioxidantes/farmacologia , Ácido Ascórbico/química , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células HeLa , Humanos , Peso Molecular , Tamanho da Partícula , Água/química
4.
Pharm Nanotechnol ; 6(1): 61-68, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29141577

RESUMO

BACKGROUND: Selenium nanoparticles (SeNPs) have gained significant importance because of its bioavailability, least toxicity, its interaction with proteins and its biocompatibility. OBJECTIVE: In the present study, the extracellular synthesis of SeNPs was carried out by using culture supernatant of Streptomyces griseoruber, an Actinomycetes member isolated from the soil and cytotoxicity was tested on HT-29 cell line. METHODS: Culture supernatant was mixed with 1mM sodium selenite for the biosynthesis of SeNPs. Characterisation of the synthesised SeNPs was done by UV-Visible spectrophotometer, FTIR, XRD, DLS and HR-TEM. The cytotoxicity of nanoparticles on HT-29 cell line was studied by MTT assay and with different staining procedure. RESULTS: Bioreduction of SeNPs was confirmed by UV-Visible spectrophotometer that showed the peak at 575 nm. Size and distribution of the biosynthesised SeNPs were analysed by HR-TEM that showed the formation of particle size in the range of 100-250nm. The synthesised SeNPs showed good cytotoxic activity against HT-29 cell line with 40.5%, 33% and 23.7% of cell viability at 2µg/ ml, 4µg/ml and 30µg/ml concentration respectively. CONCLUSION: The present study reports the simple and eco-friendly synthesis of SeNPs that showed good cytotoxic activity against HT-29 cell line suggesting that biogenic SeNPs could be a potential chemotherapeutic agent.


Assuntos
Actinomyces/metabolismo , Nanopartículas/química , Selênio/química , Antineoplásicos/química , Antineoplásicos/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células HT29 , Humanos , Tamanho da Partícula , Selenito de Sódio/química
5.
Int J Nanomedicine ; 12: 6841-6855, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28979122

RESUMO

The aim of this study was to synthesize selenium nanoparticles (SeNPs) using cell suspension and total cell protein of Acinetobacter sp. SW30 and optimize its synthesis by studying the influence of physiological and physicochemical parameters. Also, we aimed to compare its anticancer activity with that of chemically synthesized SeNPs in breast cancer cells. Cell suspension of Acinetobacter sp. SW30 was exposed to various physiological and physicochemical conditions in the presence of sodium selenite to study their effects on the synthesis and morphology of SeNPs. Breast cancer cells (4T1, MCF-7) and noncancer cells (NIH/3T3, HEK293) were exposed to different concentrations of SeNPs. The 18 h grown culture with 2.7×109 cfu/mL could synthesize amorphous nanospheres of size 78 nm at 1.5 mM and crystalline nanorods at above 2.0 mM Na2SeO3 concentration. Polygonal-shaped SeNPs of average size 79 nm were obtained in the supernatant of 4 mg/mL of total cell protein of Acinetobacter sp. SW30. Chemical SeNPs showed more anticancer activity than SeNPs synthesized by Acinetobacter sp. SW30 (BSeNPs), but they were found to be toxic to noncancer cells also. However, BSeNPs were selective against breast cancer cells than chemical ones. Results suggest that BSeNPs are a good choice of selection as anticancer agents.


Assuntos
Acinetobacter/metabolismo , Antineoplásicos/farmacologia , Nanopartículas Metálicas/química , Compostos de Selênio/síntese química , Compostos de Selênio/farmacologia , Acinetobacter/química , Animais , Antineoplásicos/síntese química , Antineoplásicos/química , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Química Verde , Células HEK293 , Humanos , Camundongos , Células NIH 3T3 , Compostos de Selênio/química , Selenito de Sódio/química , Espectroscopia de Infravermelho com Transformada de Fourier
6.
Chem Biol Interact ; 275: 74-85, 2017 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-28757135

RESUMO

New effective treatment for human non-small cell lung cancer (NSCLC) is needed. The thioredoxin (Trx) system composes of thioredoxin reductase (TrxR), Trx and NADPH. In this study, we combined an organic selenium compound--TrxR inhibitor ethaselen (BBSKE) with low dosage sodium selenite to inhibit proliferation and induce death of NSCLC cells, and identified underlying mechanisms. Synergistic anti-proliferation effect of BBSKE and selenite was found in human NSCLC cell lines, A549, NCI-H1299 and NCI-1266. A significant increase of apoptosis, necrosis and autophagy were observed in the group of BBSKE plus selenite in A549 cells. The autophagy induced by BBSKE and selenite inhibited apoptosis and necrosis. In addition, BBSKE plus selenite induced G2/M arrest, which was verified by the alteration of gene and protein expression of cell cycle regulatory complexes. The intracellular enzyme activity of TrxR was remarkably decreased by cotreatment of BBSKE and selenite. Besides, the mRNA and protein level of TrxR1 and Trx1 were significantly inhibited by cotreatment of BBSKE and selenite. HEK 293 cells overexpressing TrxR1 were more sensitive to BBSKE plus selenite. The nuclear translocation of Trx1 and Ref-1, as well as expression of Ref-1 and AP-1 were inhibited by combination treatment. In short, BBSKE synergizes selenite in inhibiting proliferation and inducing death of NSCLC cells; BBSKE combined with selenite may be a treatment strategy for NSCLC.


Assuntos
Apoptose/efeitos dos fármacos , Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia , Compostos Organosselênicos/farmacologia , Selenito de Sódio/farmacologia , Tiorredoxina Dissulfeto Redutase/antagonistas & inibidores , Células A549 , Antineoplásicos/química , Antineoplásicos/farmacologia , Autofagia/efeitos dos fármacos , Compostos Bicíclicos Heterocíclicos com Pontes/química , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Sinergismo Farmacológico , Células HEK293 , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Proteínas Associadas aos Microtúbulos/metabolismo , Compostos Organosselênicos/química , Proteínas de Ligação a RNA/metabolismo , Selenito de Sódio/química , Tiorredoxina Dissulfeto Redutase/metabolismo , Tiorredoxinas/genética , Tiorredoxinas/metabolismo , Fator de Transcrição AP-1/genética , Fator de Transcrição AP-1/metabolismo
7.
Sci Rep ; 7(1): 3239, 2017 06 12.
Artigo em Inglês | MEDLINE | ID: mdl-28607388

RESUMO

In the present study, we examined the ability of Enterobacter cloacae Z0206 to reduce toxic sodium selenite and mechanism of this process. E. cloacae Z0206 was found to completely reduce up to 10 mM selenite to elemental selenium (Se°) and form selenium nanoparticles (SeNPs) under aerobic conditions. The selenite reducing effector of E. cloacae Z0206 cell was to be a membrane-localized enzyme. iTRAQ proteomic analysis revealed that selenite induced a significant increase in the expression of fumarate reductase. Furthermore, the addition of fumarate to the broth and knockout of fumarate reductase (frd) both significantly decreased the selenite reduction rate, which revealed a previously unrecognized role of E. cloacae Z0206 fumarate reductase in selenite reduction. In contrast, glutathione-mediated Painter-type reactions were not the main pathway of selenite reducing. In conclusion, E. cloacae Z0206 effectively reduced selenite to Se° using fumarate reductase and formed SeNPs; this capability may be employed to develop a bioreactor for treating Se pollution and for the biosynthesis of SeNPs in the future.


Assuntos
Enterobacter cloacae/metabolismo , Nanopartículas/química , Selênio/química , Selenito de Sódio/metabolismo , Aerobiose , Enterobacter cloacae/enzimologia , Oxirredução , Selenito de Sódio/química , Succinato Desidrogenase
8.
Talanta ; 171: 262-269, 2017 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-28551139

RESUMO

It has been broadly recognized that the antagonism between selenium (Se) and mercury (Hg) can reduce the toxicity of mercury in organism. Glutathione (GSH) can participate in the metabolism of Se and Hg in vivo and promote the formation of low-toxic Hg-Se complexes, which is a vital way of detoxification for Hg. In this paper, the reaction mechanism of GSH-Se(IV) binary system, GSH-Hg(II) binary system and GSH-Se(IV)-Hg(II) ternary system were systematically studied from the aspects of stoichiometry, thermodynamics and kinetics, via hyphenated techniques including high performance liquid chromatography (HPLC)-ultraviolet (UV) detection, HPLC-inductively coupled plasma mass spectrometry (ICP-MS) and HPLC-electrospray ionization mass spectrometry (ESI-MS). For GSH-Se(IV) binary system, selenodiglutathione (GSSeSG) was the crucial intermediate; the reaction was exothermic and irreversible at constant pressure; it followed second-order kinetics with a fast kinetics (rate constant (k)=4534.2mol-1Ls-1). For GSH-Se(IV)-Hg(II) ternary system, GSSeSeSG would form by the extremely weak dissociation of two molecules of GSSeSG; Hg(II) would rapidly coordinate with GSSeSeSG to generate (HgxSey)n(GS)m precipitates. The mechanism of GSH-Se(IV)-Hg(II) antagonism system involves two processes, the competitive combination of Hg and Se with GSH and the formation of (HgxSey)n(GS)m complexes.


Assuntos
Glutationa/química , Cloreto de Mercúrio/química , Cloreto de Mercúrio/farmacologia , Selenito de Sódio/química , Selenito de Sódio/farmacologia , Ligação Competitiva , Cinética , Reprodutibilidade dos Testes , Termodinâmica
9.
J Anim Sci ; 95(1): 120-128, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28177352

RESUMO

Two experiments were conducted to evaluate the Se status of weaned calves (Exp. 1) and pregnant cows and newborn calves (Exp. 2) consuming bermudagrass [ (L.) Pers.] fertilized with Se. Sodium selenate was dissolved into water (8.8 g/L) and sprayed onto hayfields. Selenium-fertilized forage had greater ( ≤ 0.001) Se concentration compared with the control forage without Se fertilization (7.7 ± 1.81 vs. 0.1 ± 0.04 mg Se/kg DM and 10.8 vs. 0.1 mg Se/kg DM for Exp. 1 and Exp. 2, respectively). In Exp. 1, beef calves ( = 32; 176 ± 8.7 kg initial BW) were stratified by BW and randomly assigned to pens (16 pens; 2 calves/pen). Treatments were randomly assigned to pens, including control (no supplemental Se), Se hay, or sodium selenite ( = 2, 7, and 7 pens, respectively). A 42-d pair-feeding design was used, wherein each pen receiving Se hay was paired to a pen receiving sodium selenite. Blood and liver samples were collected on d 0, 21, and 42. Liver Se concentrations were greatest ( ≤ 0.005) on d 42 for calves provided Se hay compared with calves provided sodium selenite or control. This difference was attributed only to paired-feeding groups consuming <3 mg Se daily. In Exp. 2, mature, late-pregnancy cows were randomly assigned to 3 treatments: Se hay ( = 12), sodium selenite ( = 9), or control (no supplemental Se; = 6). Cows assigned to the sodium selenite and Se hay treatments were provided 2.5 mg of supplemental Se daily. Upon enrollment, cows were moved into individual feeding areas at an estimated 30 d prior to calving. Following calving, cotyledon and colostrum samples were collected from cows. Four days later, liver and blood samples were collected from both cows and calves. Selenium-supplemented cows had greater ( ≤ 0.001) liver, cotyledon, plasma, and whole blood Se concentrations compared with cows not receiving supplemental Se. Furthermore, cows provided Se hay tended ( = 0.11) to have greater liver Se concentrations compared with cows provided sodium selenite. Calves born to Se-supplemented cows had greater ( = 0.001) plasma Se concentrations than calves born to cows receiving no supplemental Se. Furthermore, calves born to cows provided Se hay tended ( = 0.06) to have greater plasma Se concentrations compared with calves born to cows provided sodium selenite. These data imply that Se biofortification of hayfields is an effective method to increase Se concentration of forage. Consumption of these forages result in increased Se status of weaned calves, periparturient cows, and their calves.


Assuntos
Ração Animal , Biofortificação , Bovinos/fisiologia , Cynodon/metabolismo , Selênio/metabolismo , Selenito de Sódio/metabolismo , Fenômenos Fisiológicos da Nutrição Animal , Animais , Cynodon/química , Dieta/veterinária , Suplementos Nutricionais , Feminino , Gravidez , Selênio/sangue , Selenito de Sódio/química , Desmame
10.
Anticancer Agents Med Chem ; 17(5): 658-661, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-27281365

RESUMO

Selenium (Se) is a ubiquitous, albeit not uniformly distributed metalloid present in earth crust. Consequently, its human intake with food products, particularly grains and vegetables, is also very uneven, and in certain cases can result in a severe Se deficiency. It was also documented that Se deficiency observed in some countries and/or geographic regions (e.g. Keshan region in China), is associated with an increased morbidity and mortality of neoplastic diseases. To correct this problem a number of organic and inorganic selenium compounds were developed and tested. However, it is now firmly established that only an inorganic sodium selenite with four-valent Se, and not that with six-valent (selenate) cation shows anticancer activity. This difference in their biological activities is due to their physicochemical properties. Thus selenite (Se+4) can undergo redox reaction, for example with protein's sulfhydryl groups expressed on the surface of tumor cells. In this way selenite prevents non-enzymatic formation of parafibrin that coats tumors cells and hence presents them as 'self' to the innate cellular immune system. Consequently, macrophages of the lymphatic system do not recognize neoplastic cells as 'foreign' bodies and spare them from the immune destruction. This mechanism can explain the failure of various immunotherapies to completely eliminate tumors from human bodies. Another contributing factor to carcinogenesis is the excessive consumption of red meat containing redox-active iron (Fe+3) that initiates parafibrin formation from blood fibrinogen. In conclusion, sodium selenite is a readily available and inexpensive drug of choice in the cancer treatment and prevention.


Assuntos
Antineoplásicos/farmacologia , Selenito de Sódio/farmacologia , Antineoplásicos/química , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Selenito de Sódio/química , Relação Estrutura-Atividade
11.
J Anim Sci ; 94(8): 3399-3408, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27695792

RESUMO

Angus × Simmental cows ( = 48; BW = 595 ± 17.4 kg, BCS = 5.26 ± 0.05, and age = 2.3 ± 0.07 yr), pregnant with male fetuses, were used to determine the effect of Se source during the last 80 d of gestation and first 108 d of lactation on cow and calf performance. At 203 d in gestation, cows were blocked by BW, breed composition, age, and calf sire and randomly allotted to organic Se, inorganic Se, or no Se treatments. Diets contained corn silage, corn stover, haylage, dried distillers' grains with solubles, and minerals and were formulated to contain 10.4% CP and 0.90 Mcal/kg NEg during gestation and 12.1% CP and 1.01 Mcal/kg NEg during lactation. Diets were fed daily as a total mixed ration and none, 3 mg/d Se as sodium selenite, or 3 mg/d Se as Sel-Plex were top-dressed daily. At 68 d postpartum (DPP), milk production was calculated using the weigh-suckle-weigh procedure and a milk sample was collected to determine composition. At 108 DPP, cow-calf pairs were commingled until weaning at 210 DPP. Cow BW and BCS ( ≥ 0.56) did not differ between treatments at any time point during the study. Milk production, milk fat, and total solids ( ≥ 0.38) did not differ among treatments. Milk protein tended to increase in cows fed inorganic Se compared with cows fed organic Se ( = 0.07) and milk lactose tended to be greatest in cows fed organic Se ( = 0.10). Conception to AI and overall pregnancy rates did not differ between treatments ( ≥ 0.39). Calf weights and ADG did not differ through 108 DPP ( ≥ 0.77) or for the preweaning period ( ≥ 0.33). Plasma Se concentration was adequate for all cows and did not differ among treatments for cows ( ≥ 0.37) or calves ( ≥ 0.90). Liver Se concentrations in cows fed inorganic or organic Se were greater than in control cows ( < 0.01). Longissimus muscles biopsies taken from progeny at 108 DPP also did not differ between treatments ( = 0.45). In conclusion, dietary Se source did not affect cow performance, milk production, or reproductive efficiency. Organic Se decreased milk protein and increased milk lactose but did not alter preweaning performance of progeny from Se-adequate cows.


Assuntos
Bovinos/fisiologia , Suplementos Nutricionais , Lactação/fisiologia , Selênio/farmacologia , Selenito de Sódio/farmacologia , Ração Animal/análise , Animais , Bovinos/crescimento & desenvolvimento , Dieta/veterinária , Feminino , Masculino , Leite/metabolismo , Período Pós-Parto/fisiologia , Gravidez , Reprodução/fisiologia , Selênio/administração & dosagem , Selênio/química , Selenito de Sódio/administração & dosagem , Selenito de Sódio/química , Desmame , Zea mays
12.
Carbohydr Polym ; 153: 302-311, 2016 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-27561500

RESUMO

Chuanminshen violaceum polysaccharides (CVPS) were extracted, purified and selenizingly modified. The modification has been achieved by using the HNO3- Na2SeO3 method, and selenizing Chuanminshen violaceum polysaccharides (sCVPS) were evaluated for their physicochemical properties and their potential as adjuvant to modulate cellular and humoral immune responses to hepatitis B subunit vaccine in a mouse model. Our results demonstrated that sCVPS significantly promoted splenocytes proliferation and the production of IL-4 and IFN-γ in vitro. In vivo experiments showed that sCVPS significantly increased the rHBsAg-specific IgG level, IgG subclass (IgG1, IgG2a, and IgG2b) antibody titers, T cells proliferation, levels of IL-4, IL-2, and IFN-γ in CD4 (+)T cells and the level of IFN-γ in CD8(+)T cells. Furthermore, sCVPS increased the activity of natural killer cells and cytotoxic T lymphocytes, thus increasing both cellular and humoral immune responses in vivo. The present data suggest that selenylation of CVPS can significantly improve their immune-enhancing activity both in vitro and in vivo, thus representing a powerful adjuvant for vaccine design.


Assuntos
Adjuvantes Imunológicos/farmacologia , Apiaceae/química , Vacinas contra Hepatite B/imunologia , Polissacarídeos/química , Polissacarídeos/imunologia , Selenito de Sódio/química , Adjuvantes Imunológicos/química , Animais , Citocinas/imunologia , Feminino , Vacinas contra Hepatite B/química , Humanos , Imunidade Celular/efeitos dos fármacos , Imunidade Humoral/efeitos dos fármacos , Imunoglobulina G/imunologia , Células Matadoras Naturais/imunologia , Camundongos , Ácido Nítrico/química , Polissacarídeos/isolamento & purificação , Linfócitos T Citotóxicos/imunologia , Vacinas de Subunidades/imunologia
13.
J Trace Elem Med Biol ; 37: 111-116, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27267968

RESUMO

A significant percentage of cancer patients develop secondary lymphedema after surgery or radiotherapy. The preferred treatment of secondary lymphedema is complex physical therapy. Pharmacotherapy, for example with diuretics, has received little attention, because they were not effective and only offered short-term solutions. Sodium selenite showed promise as a cost-effective, nontoxic anti-inflammatory agent. Treatment with sodium selenite lowers reactive oxygen species (ROS) production, causes a spontaneous reduction in lymphedema volume, increases the efficacy of physical therapy for lymphedema, and reduces the incidence of erysipelas infections in patients with chronic lymphedema. Besides biological effects in reducing excessive production of ROS, sodium selenite also displays various pharmacological effects. So far the exact mechanisms of these pharmacological effects are mostly unknown, but probably include inhibition of adhesion protein expression.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Linfedema/tratamento farmacológico , Selenito de Sódio/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/uso terapêutico , Humanos , Linfedema/metabolismo , Linfedema/patologia , Modalidades de Fisioterapia , Complexo Glicoproteico GPIb-IX de Plaquetas/biossíntese , Espécies Reativas de Oxigênio/metabolismo , Selenito de Sódio/química , Selenito de Sódio/uso terapêutico
14.
Zhong Yao Cai ; 39(9): 1960-5, 2016 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-30207650

RESUMO

Objective: To study the influence of different concentrations of selenium on the growth and absorbing of Se,Cd,Pb,Hg and As in Trillium tschonoskii. Methods: Trillium tschonoskii was treated with different concentrations of exogenous selenium, arsenic and heavy metals,and then the mass growth, leaf area,root number and other indicators reflecting its growth rule were calculated. Atomic fluorescence method was used to measure the absorption contents of Se,Cd,Pb,Hg and As. Results: The relative mass growth,leaf area and root number of Trillium tschonoskii increased at first and then decreased with the increasing of exogenous selenium concentrations. When the concentration of selenium was 25 mg / kg,the relative mass growth,leaf area and root number of Trillium tschonoskii reached a maximum. When selenium concentrations was over than 30 mg / kg,it inhibited the growth and development of Trillium tschonoskii. Trillium tschonoskii absorbing Cd,Pb,Hg and As had a regular of first decreasing then increasing and last decreasing. It reached the lowest when selenium concentrations at the range of 10 ~ 15 mg / kg. . Conclusion: Selenium had both stimulating effect and inhibiting effect on the growth and development of Trillium tschonoskii. Different selenium concentrations have different effects in absorbing Cd,Pb,Hg and As of Trillium tschonoskii


Assuntos
Selenito de Sódio/química , Arsênico , Metais Pesados , Selênio , Trillium
15.
Biol Trace Elem Res ; 171(1): 224-34, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26432450

RESUMO

Epimedium polysaccharide (EPS) and isatis root polysaccharide (IRPS) were extracted, purified, and selenizingly modified by nitric acid-sodium selenite method to obtain nine selenizing EPSs (sEPSs), sEPS1-sEPS9 and nine selenizing IRPSs (sIRPSs), sIRPS1-sIRPS9, respectively. Their effects on chicken peripheral lymphocyte proliferation in vitro were compared by MTT assay. The results showed that selenium polysaccharides at appropriate concentration could promote lymphocyte proliferation more significantly than unmodified polysaccharides, sEPS5 and sIRPS5 with stronger actions were picked out and injected into the chickens vaccinated with Newcastle disease vaccine in vivo tests. The peripheral lymphocyte proliferation and serum antibody titer were determined. The results showed that sEPS5 and sIRPS5 could elevate serum antibody titer and promote lymphocyte proliferation more significantly than unmodified polysaccharides, sEPS5 possessed the strongest efficacy. These results indicate that selenylation modification can significantly enhance the immune-enhancing activity of EPS and IRPS, and sEPS5 can be as a new-type immunopotentiator of chickens.


Assuntos
Galinhas/imunologia , Epimedium/química , Isatis/química , Raízes de Plantas/química , Polissacarídeos/química , Polissacarídeos/imunologia , Selenito de Sódio/química , Animais , Proliferação de Células/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Masculino , Polissacarídeos/administração & dosagem , Polissacarídeos/isolamento & purificação , Selenito de Sódio/farmacologia
16.
Arch Iran Med ; 19(1): 23-9, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26702744

RESUMO

BACKGROUND: Sodium selenite and ginsenoside Rh2 (G-Rh2) are well known for their anticancer properties and have been exploited as a new therapeutic approach. In this study, we are interested to evaluate if sodium selenite and G-Rh2 combination results in a synergistic anticancer effect that could contribute to lower systemic toxicity. METHODS: We observed the synergistic antitumor effect by combination of sodium selenite and G-Rh2 on HCT-116 human colorectal carcinoma cells in vitro. Cell growth, viability, cell cycle progression and cell apoptosis, Bax/Bcl2 ratio, caspase-3 expression, reactive oxygen species (ROS) production and autophagy were evaluated. RESULTS: The results showed that sodium selenite and G-Rh2 combination have a synergistic effect on cell growth inhibition (57%) compared with sodium selenite (25%) and G-Rh2 alone (28%) after 24 hours of treatment. This combination also induced G1 and S phase arrest simultaneously and increased apoptosis rate. The results also indicated that Bax/Bcl2 ratio and caspase-3 expression, known as proapoptotic factors, were increased in the presence of sodium selenite and G-Rh2 alone. However, combined drug treatment results in a more significant increase in Bax/Bcl2 ratio and caspase-3 expression (P < 0.05). In addition, this combination significantly induces a depletion of ROS production and autophagy, compared to control, sodium selenite and G-Rh2 alone (P < 0.05). CONCLUSION: Sodium selenite and ginsenoside Rh2 combination may be a more effective treatment for human colorectal carcinoma and is a promising chemotherapeutic approach for malignant tumors.


Assuntos
Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Ginsenosídeos/química , Espécies Reativas de Oxigênio/metabolismo , Selenito de Sódio/química , Caspase 3/metabolismo , Técnicas de Cultura de Células , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Células HCT116/efeitos dos fármacos , Humanos , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Proteína X Associada a bcl-2/metabolismo
17.
Int J Biol Macromol ; 81: 249-58, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26261081

RESUMO

Lentinan was successfully modified with nitric acid-sodium selenite method based on L9(3(4)) orthogonal experiments. The optimum selenizing conditions were obtained according to selenium conversion rate as follows: Lentinan of 1.0g, pH of 4.5, temperature of 70°C and sodium selenite of 1.50g. The antioxidant activity assays in vitro (DPPH, reducing power, superoxide radicals and hydroxyl radicals) proved that Lentinan had stronger antioxidant activity after selenizing. The elevations of serum alanine aminotransferase and aspartate aminotransferase, as well as the abnormal hepatic architecture, verified that oral administration of Seleno-Lentinan (SL2-1) markedly alleviated oxidative damage in the liver of mice induced by D-gal. In addition, SL2-1 significantly increased total antioxidant capacity, activities and protein expressions of catalase and glutathione peroxidase and lowered malondialdehyde levels in serum and liver. Fourier transform infrared spectroscopy analysis indicated that selenium of SL2-1 was mostly existed as the formations of OSeO, SeO and SeOC. Scanning electron microscope coupled with energy dispersive X-ray spectroscopy analysis revealed that the surface structure and elemental components of Lentinan significantly changed after selenizing. The results are instructive for the development of organic selenium-supplement resource.


Assuntos
Lentinano/química , Selênio/química , Animais , Antioxidantes/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/farmacologia , Concentração de Íons de Hidrogênio , Lentinano/isolamento & purificação , Lentinano/farmacologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Masculino , Camundongos , Oxirredução/efeitos dos fármacos , Selenito de Sódio/química , Espectroscopia de Infravermelho com Transformada de Fourier , Superóxidos/antagonistas & inibidores , Superóxidos/química , Temperatura Ambiente
18.
Fitoterapia ; 105: 73-82, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26079445

RESUMO

Black chokeberry has been known to play a protective role in human health due to its high polyphenolic content including anthocyanins and caffeic acid derivatives. In the present study, we first characterized the polyphenolic content of a commercial chokeberry concentrate and investigated its effect on LPS-induced NF-κB activation and release of pro-inflammatory mediators in macrophages in the presence or the absence of sodium selenite. Examination of the phytochemical profile of the juice concentrate revealed high content of polyphenols (3.3%), including anthocyanins, proanthocyanidins, phenolic acids, and flavonoids. Among them, cyanidin-3-O-galactoside and caffeoylquinic acids were identified as the major compounds. Data indicated that chokeberry concentrate inhibited both the release of TNFα, IL-6 and IL-8 in human peripheral monocytes and the activation of the NF-κB pathway in RAW 264.7 macrophage cells. Furthermore, chokeberry synergizes with sodium selenite to inhibit NF-κB activation, cytokine release and PGE2 synthesis. These findings suggest that selenium added to chokeberry juice enhances significantly its anti-inflammatory activity, thus revealing a sound approach in order to tune the use of traditional herbals by combining them with micronutrients.


Assuntos
Inflamação/metabolismo , Macrófagos/efeitos dos fármacos , NF-kappa B/antagonistas & inibidores , Photinia/química , Polifenóis/química , Selênio/química , Animais , Células Cultivadas , Dinoprostona/metabolismo , Sinergismo Farmacológico , Sucos de Frutas e Vegetais , Humanos , Interleucina-6/metabolismo , Interleucina-8/metabolismo , Macrófagos/metabolismo , Camundongos , Monócitos/efeitos dos fármacos , Monócitos/metabolismo , NF-kappa B/metabolismo , Compostos Fitoquímicos/química , Células RAW 264.7 , Transdução de Sinais/efeitos dos fármacos , Selenito de Sódio/química , Fator de Necrose Tumoral alfa/metabolismo
19.
Biol Trace Elem Res ; 165(2): 145-52, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25634140

RESUMO

One hundred and eighty Kunming mice were allotted to three groups in a randomized complete block design, including two treatments and one control. Mice in group 1 were fed a basal diet as control, while mice in groups 2 and 3 were fed the basal diet supplemented with 0.2 mg/kg selenium as sodium selenite (SS) or selenium-chitosan (SC), respectively. On day 28 of the experiment, blood selenium concentration, glutathione peroxidase (GPx) activity, plasma superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and Con A-induced splenocyte proliferation were determined, and plasma interleukin-2 (IL-2) and interferon-γ (IFN-γ) concentrations, splenic plaque-forming cell (PFC) responses, serum hemolysis level (HC50), and delayed-type hypersensitivity (DTH) responses were determined on day 15 of the experiment. The results showed that blood selenium concentration, GPx activity, splenic PFC response, and plasma IL-2 and IFN-γ concentrations in SC group were higher than those in the control and SS groups (P < 0.01 or P < 0.05), respectively. Plasma SOD activity, Serum hemolysis level, DTH responses, and Con A-induced splenocyte proliferation in SC group were higher than those in control (P < 0.01 or P < 0.05). Plasma SOD activity, serum hemolysis level, DTH responses, and Con A-induced splenocyte proliferation in SC group were also higher than those in SS group, while there was no significant difference between SC and SS groups (P > 0.05). Plasma MDA content in SC group was lower than those in the control and SS groups (P < 0.01 or P < 0.05). It is concluded that SC supplement can increase blood selenium concentration, antioxidation status, and cellular and humoral immunity, and SC has better biological activity than SS in mice.


Assuntos
Antioxidantes/metabolismo , Quitosana/química , Imunidade Celular/efeitos dos fármacos , Imunidade Humoral/efeitos dos fármacos , Compostos de Selênio/química , Selênio/sangue , Selenito de Sódio/química , Animais , Proliferação de Células , Eritrócitos/citologia , Feminino , Glutationa Peroxidase/metabolismo , Hemólise , Interferon gama/sangue , Interferon gama/metabolismo , Interleucina-2/sangue , Interleucina-2/metabolismo , Masculino , Camundongos , Distribuição Aleatória , Selênio/química , Ovinos , Baço/citologia , Superóxido Dismutase
20.
Carbohydr Polym ; 103: 148-53, 2014 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-24528713

RESUMO

Lycium barbarum polysaccharide (LBP) was modified by HNO3-Na2SeO3 method according to L9(3(4)) orthogonal design to obtain nine selenizing LBPs (sLBPs), sLBP1-sLBP9. Their antioxidant activities in vitro were compared by free radical-scavenging test. sLBP6, sLBP8 and sLBP9 presented stronger activity. In vivo test, 14-day-old chickens were injected respectively with sLBP6, sLBP8 and sLBP9 taking LBP as control, and serum GSH-Px and SOD activities and MDA content were determined. The results showed that three sLBPs could significantly enhance GSH-Px and SOD activities and decrease MDA content. The actions of sLBPs were significantly stronger than that of unmodified LBP. These results indicated that selenylation modification could significantly enhance the antioxidant activities of LBP, sLBP6 possessed the best efficacy and could be exploited into an antioxidant. The optimal modification conditions were 400mg of sodium selenite for 500 mg of LBP, reaction temperature of 70 °C and reaction time of 6h.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Selenito de Sódio/química , Animais , Antioxidantes/administração & dosagem , Galinhas , Medicamentos de Ervas Chinesas/administração & dosagem , Glutationa Peroxidase/metabolismo , Lycium/química , Ácido Nítrico/química , Superóxido Dismutase/metabolismo
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