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1.
J Dairy Sci ; 103(2): 1969-1981, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31864734

RESUMO

Eleven mid-lactation Holstein cows were milked twice daily during the first 2 experimental weeks. During wk 3 to 10, the cows were differentially milked: right quarters were milked thrice daily (3×) and left quarters were milked once daily (1×). During wk 11 to 14, all quarters were milked twice daily. After 4 wk of differential milking, the cows received daily i.m. injections of the dopamine antagonist domperidone (DOMP; 300 mg; n = 6) or of dimethyl sulfoxide as the control (CTL; n = 5) for 8 wk (wk 7-14). During the differential milking period (wk 3-6), milk production was greater for quarters milked 3× than for those milked 1× but did not differ between DOMP and CTL cows. During the differential milking + injection period (wk 7-10), milk production continued to differ according to milking frequency. However, DOMP injection did not have an effect or interact with milking frequency on milk production. During the injection period (wk 11-14), milk production remained greater in the quarters previously milked 3× and milk production increased in DOMP injected cows but not in CTL cows. Injections of DOMP increased prolactin concentration, which was greater in the serum of DOMP cows than in that of CTL cows during the differential milking + injection and the injection periods. The expression of genes that are directly related to milk synthesis (CSN2, LALBA, and ACACA) was greater in the 3× quarters than in the 1× quarters. In addition, DOMP increased CSN2 expression during the injection period. The expression of both isoforms of the PRLR gene was greater in the 3× quarters during the differential milking + injection and the injection periods. At the protein level, injections of DOMP tended to increase the number of long PRLR isoform during the differential milking + injection period. The number of short PRLR isoform was greater in the 1× quarters than in the 3× quarters during the differential milking, the differential milking + injection, and the injection periods. The total amount of STAT3 protein was greater in the 1× quarters during the differential milking and the differential milking + injection periods. The amount of phosphorylated STAT3 protein was greater in the 1× quarters during the differential milking period. The total amount of phosphorylated STAT5 protein was greater in the 3× quarters during the differential milking and the differential milking + injection periods. The results of this experiment support the hypothesis that the responsiveness of the mammary gland to PRL is modulated by milking frequency, although the underlying mechanism remains to be determined.


Assuntos
Bovinos/fisiologia , Indústria de Laticínios/métodos , Domperidona/administração & dosagem , Antagonistas de Dopamina/administração & dosagem , Leite/metabolismo , Prolactina/sangue , Transdução de Sinais/efeitos dos fármacos , Animais , Feminino , Lactação/efeitos dos fármacos , Glândulas Mamárias Animais/fisiologia , Leite/química , Fosforilação , Prolactina/efeitos dos fármacos , Fator de Transcrição STAT5/metabolismo , Serotonina/análise , Fatores de Tempo
2.
Folia Parasitol (Praha) ; 662019 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-31366737

RESUMO

The localisation and distribution of the serotoninergic nerve elements was studied for the first time in the flatworm Chimaericola leptogaster (Leuckart, 1830) using immunocytochemical methodology and confocal laser scanning microscopy. The musculature was investigated by histochemical staining of actin filaments; scanning electron microscopy was used to identify the sensory structures on the worm's surface. Uniciliated, bi-ciliated and multiciliated sensory endings have been described on the worm's surface. The morphological data demonstrate the presence of circular, longitudinal and diagonal muscles that comprise the musculature of C. leptogaster in the anterior, median and posterior body regions. Well-developed radial and circular muscle fibres were also observed surrounding the genital pore, two vaginae and in clumps of the haptor. The study revealed the presence of biogenic amine, serotonin, in the central and peripheral nervous systems of C. leptogaster: in the neurons and fibres of the cephalic ganglia and ventral nerve cord, in the innervation of reproductive system compartments. The localised sites of the serotoninergic elements point to important roles of serotonin in monogenean reproductive processes and, possibly, in the regulation of muscle function.


Assuntos
Peixes/parasitologia , Fenômenos Fisiológicos do Sistema Nervoso , Serotonina/análise , Trematódeos/fisiologia , Animais , Feminino , Imuno-Histoquímica/veterinária , Masculino , Microscopia Confocal/veterinária , Microscopia Eletrônica de Varredura/veterinária , Músculos/citologia , Músculos/ultraestrutura , Sistema Nervoso/citologia , Sistema Nervoso/ultraestrutura , Trematódeos/citologia , Trematódeos/ultraestrutura
3.
Am J Clin Pathol ; 152(6): 718-724, 2019 11 04.
Artigo em Inglês | MEDLINE | ID: mdl-31365739

RESUMO

OBJECTIVES: Analysis of platelet functional responses to stimuli is presently quite limited with respect to measurement of dense granule secretion. We sought to develop a nonradioactive assay of stimulated serotonin release using liquid chromatography tandem mass spectrometry (LC-MS/MS). METHODS: Citrated whole blood (200 µL) was incubated with deuterated serotonin (d45-HT). Following uptake by platelets, blood was diluted 10-fold and aliquots were incubated with platelet stimuli. Following stimulation, blood was further diluted, centrifuged, and supernatant was assayed for released d45-HT by micro-LC-MS/MS. RESULTS: This study demonstrated a broad linear range of 50 to 2,000 pg/mL d45-HT, with a total precision of less than 15.0% coefficient of variation at all quality control levels and a limit of quantitation of 50 pg/mL. CONCLUSIONS: Quantification of d45-HT by micro-LC-MS/MS assay offers a highly sensitive, nonradioactive methodology for quantitating platelet serotonin uptake and dense granule secretion, requiring only small volumes of patient blood.


Assuntos
Cromatografia Líquida/métodos , Testes de Função Plaquetária/métodos , Serotonina/análise , Espectrometria de Massas em Tandem/métodos , Humanos , Serotonina/metabolismo
4.
Parasit Vectors ; 12(1): 397, 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31399119

RESUMO

BACKGROUND: La Crosse virus (LACV) infection has been shown to manipulate the blood-feeding behaviors of its main vector, Aedes triseriatus. Here, we investigated the effects of virus infection on serotonin and dopamine and their potential roles in host-seeking. In mosquitoes, serotonin depletion has been shown to interfere with blood-feeding but not host-seeking. Dopamine depletion does not affect either blood-feeding or host-seeking; elevations of dopamine, however, has been shown to inhibit host-seeking. The purpose of this study was to determine the effects of LACV infection on the host-seeking behavior of and neurotransmitter levels in Ae. triseriatus. METHODS: Host-seeking behavior was evaluated using a uni-port olfactometer and a membrane feeder assay. Levels of serotonin and dopamine in infected and control mosquito heads were measured using HPLC-ED. RESULTS: Infection with LACV significantly inhibited the activation and attraction of Ae. triseriatus females to a host. A higher proportion of uninfected Ae. triseriatus females were activated by the presence of a host compared to infected mosquitoes and more uninfected mosquitoes were full responders (95.7%) compared to infected ones (91.1%). However, infection with LACV did not significantly affect the landing, probing, or blood-feeding rates of female mosquitoes. LACV-infected mosquitoes had lower serotonin levels than controls (104.5 vs 138.3 pg/head) while the dopamine levels were not affected by infection status (282.3 vs 237 pg/head). CONCLUSIONS: Our work suggests that virus-induced reduction of serotonin is related to previously reported blood-feeding alterations in LACV-infected mosquitoes and could lead to enhanced transmission and increased vectorial capacity. In addition, some aspects of host-seeking were inhibited by virus infection.


Assuntos
Aedes/química , Aedes/virologia , Encefalite da Califórnia/virologia , Comportamento Alimentar , Comportamento de Busca por Hospedeiro , Neurotransmissores/análise , Animais , Dopamina/análise , Feminino , Vírus La Crosse/fisiologia , Controle de Mosquitos , Mosquitos Vetores/química , Mosquitos Vetores/virologia , Olfatometria , Serotonina/análise
5.
Biomed Chromatogr ; 33(11): e4650, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31313343

RESUMO

Solid-phase extraction technologies are widely used for sample pretreatment in bioanalysis. Monolithic silica disk-packed spin columns modified with phenylboronate moieties have been developed for the selective extraction of cis-diol compounds such as catecholamines. However, in our preliminary studies, serotonin was found to also be extracted in this treatment, along with catecholamines. In this study, the interaction between serotonin-related compounds (serotonin, tryptophan, 5-hydroxy-tryptophan and 5-hydroxyindoleacetic acid) and phenylboronate moieties was investigated. We found that only serotonin was extracted with phenylboronate-modified monolithic silica, whereas tryptophan, 5-hydroxy-tryptophan and 5-hydroxyindoleacetic acid were not. Hydrophobic interactions rather than ionic interactions were the primary factor for the adsorption of serotonin to phenylboronate. Finally, the selective pretreatment procedure for catecholamines was improved: thus, the method could be applied for the pretreatment of bio-samples.


Assuntos
Ácidos Borônicos/química , Ácido Hidroxi-Indolacético , Serotonina , Extração em Fase Sólida , Triptofano , Adsorção , Catecolaminas , Cromatografia Líquida de Alta Pressão , Interações Hidrofóbicas e Hidrofílicas , Ácido Hidroxi-Indolacético/análise , Ácido Hidroxi-Indolacético/química , Ácido Hidroxi-Indolacético/isolamento & purificação , Serotonina/análise , Serotonina/química , Serotonina/isolamento & purificação , Dióxido de Silício , Extração em Fase Sólida/instrumentação , Extração em Fase Sólida/métodos , Triptofano/análise , Triptofano/química , Triptofano/isolamento & purificação
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 223: 117319, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31280124

RESUMO

Serotonin (5-hydroxytryptamine, 5-HT) is a crucial endogenous monoamine neurotransmitter that modulates neurotransmission, gastrointestinal motility, hemostasis, and cardiovascular integrity. There have been numerous attempts to study the biochemical and photophysical properties of serotonin to carry out its molecular imaging and quantitative estimation. Here, we investigate the properties of serotonin at physiological concentration and pH using a continuous wave (CW) laser excitation closed-aperture (CA) Z-scan technique. Serotonin is packaged at high concentration inside the acidic environment of vesicles, and upon release gets diluted at the release sites in a neutral pH environment. Our solution-based measurements indicate that serotonin showed negative refractive nonlinearity and positive absorptive nonlinearity at a neutral pH. However, in the acidic medium, it showed negative refractive nonlinearity and mostly negative absorptive nonlinearity. The effect of excitation laser power on the observed nonlinearity is also verified. We attribute the origin of the nonlinearity in serotonin to the thermal lensing effect. Our robust and straightforward strategy to probe the monoamine neurotransmitter properties will provide new avenues to investigate serotonergic processes.


Assuntos
Dinâmica não Linear , Fenômenos Ópticos , Serotonina/análise , Concentração de Íons de Hidrogênio , Lasers , Refratometria , Serotonina/química , Análise Espectral
7.
Food Chem ; 298: 125020, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260965

RESUMO

Bioactive amines are found in food and can be relevant for the assessment of fruits shelf life and nutritional quality. The pulp and peel of 20 banana and plantain were analyzed and the bioactive amine content varied according to the genotype, ripening stage, fruit tissue and thermal processing. In most of the analyzed genotypes, tyramine, histamine, dopamine, serotonin, spermidine, and spermine were decreased during the ripening process in the pulps. By contrast, there was an increase in putrescine level. In many genotypes of plantains, the serotonin and dopamine contents in pulp decreased until stage 5 and increased at stage 7. Peels contain higher levels of serotonin, dopamine, histamine and tyramine than pulps. Additionally, thermal processing affects the content of amines present in fruit. Boiling with the peel should be preferred in domestic preparations, regardless of the genotype used.


Assuntos
Aminas/análise , Frutas/metabolismo , Musa/metabolismo , Plantago/metabolismo , Aminas Biogênicas/análise , Dopamina/análise , Frutas/química , Genótipo , Histamina/análise , Musa/química , Musa/genética , Plantago/química , Plantago/genética , Putrescina/análise , Serotonina/análise , Espermidina/análise , Espermina/análise , Temperatura , Tiramina/análise
8.
Anal Chim Acta ; 1077: 255-265, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31307717

RESUMO

Herein, we propose a highly sensitive and selective three-dimensional electrochemical paper-based analytical device (3D-ePAD) to determine serotonin (Ser). It uses a graphite-paste electrode modified with nanoparticles coated with molecularly imprinted polymer (MIP). Fe3O4@Au nanoparticles were encapsulated with silica to create novel nano-sized MIP. Morphology and structural characterization reveal that silica imprinted sites (Fe3O4@Au@SiO2) synthesized via sol-gel methods provide excellent features for Ser detection, including high porosity, and greatly improve analyte diffusion and adsorption to provide a faster response by the MIP sensor. The template molecule was effectively removed by solvent extraction to provide a greater number of specific cavities that enhance analyte capacity and sensitivity. The 3D-ePAD was fabricated by alkyl ketene dimer (AKD)-inkjet printing of a circular hydrophobic detection zone on filter paper for application of aqueous samples, coupled with screen-printed electrodes on the paper, which was folded underneath the hydrophobic zone. The sensor was constructed by drop coating of Fe3O4@Au@SiO2-MIP nanocomposites on the graphite electrode (GPE) surface. The MIP sensor (Fe3O4@Au@SiO2-MIP/GPE) was used in the detection of Ser by linear-sweep voltammetry (LSV) in 0.1 M phosphate buffer at pH 8.0. The device exhibits high sensitivity toward Ser, which we attribute to synergistic effects between catalytic properties, electrical conductivity of Fe3O4@Au@SiO2, and significantly increased numbers of imprinted sites. Ser oxidation was observed at +0.39 V. Anodic peak currents for Ser show linearity from 0.01 to 1000 µM (y = 0.0075 ± 0.0049 x + 0.4071 ± 0.0052, r2 = 0.993), with a detection limit of 0.002 µM (3S/N). The device provides good repeatability (%relative standard deviations; RSD) = 4.23%, calculated from the current responses of ten different MIP sensors). The device also exhibits high selectivity and reproducibility (%RSD = 8.35%, obtained from five calibration plots). The analytical performance of the device is suitable for the determination of Ser in pharmaceutical capsules and urine samples.


Assuntos
Ouro/química , Nanopartículas de Magnetita/química , Povidona/química , Serotonina/análise , Dióxido de Silício/química , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Eletrodos , Grafite/química , Limite de Detecção , Impressão Molecular , Papel
9.
Biosens Bioelectron ; 141: 111404, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31202186

RESUMO

The bio-sensory organs of living creatures have evolved to have the best sensing performance. They have 3-dimensional protrusions that have large surface areas to accommodate a large number of membrane proteins such as ion channels and G-protein coupled receptors, resulting in high sensitivity and specificity to target molecules. From the perspective of mimicking this system, BLM, which has been used extensively as a platform for a single nanopore-based sensing systems, has some limitations, i.e., some residual solvent, low mechanical stability, small surface area for appropriate stability, and difficulty in high-throughput fabrication. Herein, to eliminate these limitations, a solvent-free, size-controllable, 3-dimensional free-standing lipid bilayer (3DFLB) structure array with high stability (∼130 h) and high density (∼300,000 cm-2) is proposed, and its structural advantages for efficient and rapid protein reconstitution, compared to BLM, is demonstrated by human 5-HT3A receptor assay as well as α-hemolysin assay. A continuous process of 3DFLB array fabrication, 5-HT3A reconstitution, and 5-HT detections in a microfluidic channel proves the applicability of the proposed structures as a highly-sensitive sensing platform mimicking bio-sensory organs.


Assuntos
Técnicas Biossensoriais/instrumentação , Dispositivos Lab-On-A-Chip , Bicamadas Lipídicas/química , Receptores 5-HT3 de Serotonina/metabolismo , Serotonina/análise , Desenho de Equipamento , Humanos , Proteínas Imobilizadas/metabolismo , Proteínas Recombinantes/metabolismo , Serotonina/metabolismo
10.
Biomed Chromatogr ; 33(10): e4626, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31222753

RESUMO

N-Ethylpentylone (NEP) is a popular synthetic cathinone abused worldwide. To obtain more information about its pharmacokinetics and pharmacodynamics, a rapid, simple and sensitive liquid chromatography-tandem mass spectrometry method was developed for the determination of NEP, two important neurotransmitters, dopamine and serotonin, and their metabolites, including 3,4-dihydroxyphenylacetic acid, 3-methoxytyramine and 5-hydroxyindole-3-acetic acid, in rat brain microdialysate. The analytes were separated on a Phnomenex Polar C18 column, with a mobile phase of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B) under gradient elution to shorten the total chromatographic run time. A triple quadruple mass spectrometer coupled with an electrospray ionization source in both positive and negative ion mode was used to detect the analytes. This method showed excellent accuracy (87.4-113.5%) and precision (relative standard deviation <15%) at three quality control levels. The limits of detection were 0.2 ng/mL for NEP and 0.2-50 nm for the others and good linearity was obtained. This study pioneered a method to integrate exogenous drugs and endogenous neurotransmitters as the drugs act on the same determination system, which means that this innovation can provide support for further study of the addictive effects of NEP or other synthetic cathinones on extracellular levels of dopamine and 5-hydroxytryptamine.


Assuntos
Benzodioxóis/análise , Butilaminas/análise , Cromatografia Líquida de Alta Pressão/métodos , Dopamina/análise , Núcleo Accumbens/química , Serotonina/análise , Animais , Benzodioxóis/administração & dosagem , Benzodioxóis/farmacocinética , Butilaminas/administração & dosagem , Butilaminas/farmacocinética , Dopamina/metabolismo , Limite de Detecção , Modelos Lineares , Microdiálise , Núcleo Accumbens/metabolismo , Ratos , Reprodutibilidade dos Testes , Serotonina/metabolismo , Espectrometria de Massas em Tandem/métodos
11.
Can J Physiol Pharmacol ; 97(10): 924-931, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31132324

RESUMO

Sensitized stress-induced corticosterone (CORT) secretion in chronically stressed rats involves 5-HT7 receptor activation. The effect of 14-day chronic CORT and vehicle (VEH) administration on 5-HT7 receptor expression in adrenal glands, adrenal 5-HT content, and adrenocorticotropic hormone and CORT secretion was analysed. On day 15, VEH- and CORT-treated animals were perfused or decapitated without stress exposure (0 min) or after 10 and 30 min of restraint for collection of trunk blood and tissues. 5-HT7 receptor-like immunoreactivity (5-HT7R-LI), 5-HT7 receptor protein, and mRNA levels were determined by immunohistochemistry, Western blot, and reverse transcription polymerase chain reaction assays, respectively; 5-HT levels and hormones were quantified using HPLC and ELISA kits, respectively. An undisturbed control group was included for most experimental comparisons. Chronic CORT strongly increased 5-HT7R-LI in the outer adrenal cortex, as well as 5-HT7 receptor protein and mRNA in whole adrenal glands; adrenal 5-HT content also increased in these animals. Decreased adrenocorticotropic hormone and CORT secretion at 30 min of restraint occurred in CORT-treated rats. The results support the notion that chronic stress-induced increase of adrenocortical 5-HT7 receptors and adrenal 5-HT content is a glucocorticoid-dependent phenomenon; the development of magnified stress-induced 5-HT7 receptor-mediated CORT responses in chronically stressed animals nevertheless likely involves additional mechanisms.


Assuntos
Glândulas Suprarrenais/efeitos dos fármacos , Corticosterona/administração & dosagem , Receptores de Serotonina/metabolismo , Estresse Psicológico/metabolismo , Glândulas Suprarrenais/metabolismo , Hormônio Adrenocorticotrópico/análise , Hormônio Adrenocorticotrópico/metabolismo , Animais , Corticosterona/metabolismo , Modelos Animais de Doenças , Humanos , Sistema Hipotálamo-Hipofisário/metabolismo , Masculino , Sistema Hipófise-Suprarrenal/metabolismo , Ratos , Receptores de Serotonina/análise , Restrição Física/psicologia , Serotonina/análise , Serotonina/metabolismo , Estresse Psicológico/etiologia , Estresse Psicológico/psicologia
12.
Biosens Bioelectron ; 138: 111310, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31103014

RESUMO

In this work, we detail the progress of a novel electrochemical disposable device, which has a relatively low cost and easy production, with a novel conductive ink, that consists of graphite and automotive varnish mixture, deposited over a self-adhesive paper, granting an easy production with relatively low cost. The electrode surface was characterized by scanning electron microscopy, X-ray powder diffraction and Fourier transforms infrared and Raman, cyclic voltammetry and electrochemical impedance spectroscopies. In addition, the proposed electrode was applied for individual electrochemical determination of dopamine and serotonin. The device achieved a linear response between 30 and 800 µmol L-1 and a limit of detection (LOD) of 0.13 µmol L-1, by square wave voltammetry for dopamine and a linear range from 6.0 to 100 µmol L-1, with a LOD of 0.39 µmol L-1, by differential pulse voltammetry for serotonin. Later, the working electrode was modified with glucose oxidase and dihexadecyl phosphate film in order to obtain a biosensor. At this stage, CV was applied to detect glucose in the range of 1.0-10 µmol L-1 and LOD of 0.21 µmol L-1. By three different techniques and analytes, the sensoring and biosensoring processes presented high reproducibility. The proposed adhesive electrode is easy to prepare, disposable, within non-restrictive nature, which allows an approach of a new device for electrochemical sensing and biosensing.


Assuntos
Técnicas Biossensoriais/instrumentação , Dopamina/análise , Glucose/análise , Neurotransmissores/análise , Papel , Serotonina/análise , Espectroscopia Dielétrica , Condutividade Elétrica , Técnicas Eletroquímicas , Eletrodos , Glucose Oxidase/química , Grafite/química , Tinta , Limite de Detecção , Organofosfatos/química , Reprodutibilidade dos Testes
13.
Psychiatr Danub ; 31(1): 78-87, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30948693

RESUMO

BACKGROUND: The role of serotonin transporter and its functional gene polymorphism (5-HTTLPR, serotonin transporter linked polymorphic region) was investigated in sleep disturbances in various mental disorders, with conflicting findings. Here, the association of particular sleep disturbances with 5-HTTLPR genotypes and platelet serotonin (5-HT) concentration was determined simultaneously in veterans with posttraumatic stress disorder (PTSD), who were subdivided into those with or without comorbid depression. SUBJECTS AND METHODS: Croatian male, medication-free war veterans with PTSD (N=325), subdivided into those with or without comorbid depression, and subdivided further according to the various sleep disturbances, were evaluated using the Structured Clinical Interview, the Hamilton Rating Scale for Depression and the Clinician Administered PTSD Scale. Genotyping and platelet 5-HT concentration measurements were conducted using PCR and spectrofluorimetric methods, respectively. RESULTS: Nominally higher frequency of the 5-HTTLPR LL genotype compared to S carriers (p=0.026; χ2 test) and significantly higher platelet 5-HT concentration (p=0.001; one-way ANOVA) were detected in non-depressed veterans with PTSD with early insomnia, compared to matched veterans without early insomnia. CONCLUSIONS: Over-representation of the LL genotype of the 5-HTTLPR and higher platelet 5-HT concentrations were detected in veterans with PTSD who did not develop comorbid depression but had severe early insomnia. These results suggest that 5-HTTLPR genotypes and platelet 5-HT concentration are associated with early insomnia in non-depressed veterans with PTSD. Limitations of the study were the cross-sectional nature of the study, biallelic assessment of the 5-HTTLPR, and a lack of use of the specific sleep measurement scales. These results should be replicated in larger samples, validated on different populations, using specific sleep measurement scales and triallelic 5-HTTLPR assessment.


Assuntos
Proteínas da Membrana Plasmática de Transporte de Serotonina , Serotonina , Distúrbios do Início e da Manutenção do Sono , Transtornos de Estresse Pós-Traumáticos , Veteranos , Plaquetas/química , Estudos Transversais , Humanos , Masculino , Polimorfismo Genético , Serotonina/análise , Proteínas da Membrana Plasmática de Transporte de Serotonina/genética , Distúrbios do Início e da Manutenção do Sono/complicações , Distúrbios do Início e da Manutenção do Sono/genética , Transtornos de Estresse Pós-Traumáticos/complicações , Transtornos de Estresse Pós-Traumáticos/genética , Veteranos/psicologia
14.
Mater Sci Eng C Mater Biol Appl ; 100: 388-395, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30948075

RESUMO

Cobalt oxide nanocubes incorporated with reduced graphene oxide (rGO-Co3O4) was prepared by using simple one-step hydrothermal route. Crystallinity and structural characteristics of the nanocomposite were analyzed and confirmed using X-ray diffraction (XRD) and Raman analysis, respectively. The cubical shape of the Co3O4 nanostructures and the distribution of Co3O4 nanocubes on the surface of rGO sheets were identified through field emission scanning electron microscopy (FESEM) and energy dispersive X-ray (EDX) mapping analysis, respectively. Raman spectra depicted the presence of D and G bands for GO and rGO with different ID/IG values and thus confirmed the reduction of GO into rGO. The electrochemical study reflects that the rGO-Co3O4 nanocomposite shows good electrocatalytic activity in oxidation of depression biomarker serotonin (5-HT) in phosphate buffer (pH 7.2). The detection of 5-HT was carried out by using rGO-Co3O4 nanocomposite modified glassy carbon electrode under dynamic condition using amperometry technique with a linear range of 1-10 µM. The limit of detection and limit of quantification were calculated and found to be 1.128 and 3.760 µM, respectively with a sensitivity value of 0.133 µΑ·µM-1. The sensor showed selectivity in the presence of different interferent species such as ascorbic acid, dopamine and uric acid.


Assuntos
Carbono/química , Cobalto/química , Técnicas Eletroquímicas/métodos , Vidro/química , Grafite/química , Nanocompostos/química , Óxidos/química , Serotonina/análise , Catálise , Eletrodos , Nanocompostos/ultraestrutura , Oxirredução , Análise Espectral Raman , Difração de Raios X
15.
Ann Biol Clin (Paris) ; 77(2): 161-168, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30998196

RESUMO

OBJECTIVES: Platelet serotonin and its urinary metabolite 5-HIAA (5-hydroxyindolacetic acid) are the main biomarkers measured for the detection of neuroendocrine tumors (NET). We observe in our laboratory many false positives or false negatives for the 2 assays using threshold values given by the manufacturer. We aim to determine our own local threshold values for a better detection of gastrointestinal NETs. METHODS: We studied patients with measurement of platelet serotonin and/or urinary 5-HIAA in University Hospital of Tours between January 2005 and June 2016. We established an « index ¼ cohort with 75% of patients to determine local threshold value for the 2 parameters. A "validation" cohort constituted with 25% of remaining patients allowed us to compare the performances of manufacturer's values with local threshold values. RESULTS: Two hundred ninety patients were included, with 19 suffering from NETs. Local threshold value for platelet serotonin was determined at 5.13 amol/platelet, the one for urinary 5-HIAA at 3.60 µmol/mmol urinary creatinine. Platelet serotonin specificity was better with local threshold value for identical sensibility (0.75). Urinary 5-HIAA sensibility was improved with local threshold value (1 vs 0.667) for identical specificity (0.902). CONCLUSION: Using our local threshold value for platelet serotonin and urinary 5-HIAA improved diagnostic performances of these biochemical markers to detect NETs.


Assuntos
Análise Química do Sangue/métodos , Plaquetas/química , Neoplasias do Sistema Digestório/diagnóstico , Ácido Hidroxi-Indolacético/urina , Tumores Neuroendócrinos/diagnóstico , Serotonina/análise , Urinálise/métodos , Adulto , Idoso , Biomarcadores/sangue , Biomarcadores/urina , Análise Química do Sangue/normas , Plaquetas/metabolismo , Estudos de Coortes , Neoplasias do Sistema Digestório/sangue , Neoplasias do Sistema Digestório/urina , Feminino , Humanos , Ácido Hidroxi-Indolacético/análise , Neoplasias Intestinais/sangue , Neoplasias Intestinais/diagnóstico , Neoplasias Intestinais/urina , Masculino , Pessoa de Meia-Idade , Tumores Neuroendócrinos/sangue , Tumores Neuroendócrinos/urina , Neoplasias Pancreáticas/sangue , Neoplasias Pancreáticas/diagnóstico , Neoplasias Pancreáticas/urina , Valores de Referência , Estudos Retrospectivos , Serotonina/sangue , Neoplasias Gástricas/sangue , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/urina
16.
ACS Sens ; 4(5): 1211-1221, 2019 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-30969113

RESUMO

This article describes the device design and fabrication of two different configurations (flow-through and stopped-flow) of a sequential fluid delivery platform on a microfluidic paper-based device. The developed device is capable of storing and transporting reagents sequentially to the detection channel without the need for external power. The device comprises two components: an origami folding paper (oPAD) and a movable reagent-stored pad (rPAD). This 3D capillary-driven device eliminates the undesirable procedure of multiple-step reagent manipulation in a complex assay. To demonstrate the scope of this approach, the device is used for electrochemical detection of biological species. Using a flow-through configuration, a self-calibration plot plus real sample analysis using a single buffer introduction are established for ascorbic acid detection. We further broaden the effectiveness of the device to a complex assay using a stopped-flow configuration. Unlike other electrochemical paper-based sensors in which the user is required to cut off the device inlet or rest for the whole channel saturation before measurement, herein a stopped-flow device is carefully designed to exclude the disturbance from the convective mass transport. As a proof of concept, multiple procedures for electrode modification and voltammetric determination of serotonin are illustrated. In addition, the research includes an impedimetric label-free immunosensor for α-fetoprotein using the modified stopped-flow device. The beneficial advantages of simplicity, low sample volume (1 µL), and ability to perform a complex assay qualify this innovative device for use with diverse applications.


Assuntos
Eletroquímica/instrumentação , Dispositivos Lab-On-A-Chip , Papel , Eletrodos , Desenho de Equipamento , Ouro/química , Imunoensaio , Serotonina/análise , alfa-Fetoproteínas/análise
17.
Fitoterapia ; 134: 165-171, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30825572

RESUMO

Two novel phenanthrenoids, juncuenin H (1) and dijuncuenin B (2), together with eight known phenanthrenoids, effusol (3), dehydroeffusol (4), juncusol (5), dehydrojuncusol (6), juncuenin B (7), dehydrojuncuenin B (8), juncuenin A (9), and dehydrojuncuenin A (10), were isolated from the underground parts of Juncus setchuenensis. The structures of the compounds were determined by 1D and 2D NMR and mass spectroscopy. The anxiolytic activities of compounds 1, 6, 9, and 10 were evaluated. In order to explore the mechanisms underlying their anxiolytic activities, the levels of serotonin (5-HT), dopamine (DA), and their metabolites in the cerebral cortex and hippocampus of mice treated with compound 1 were determined by quantitative mass spectrometry. The mice treated with compound 1 had significantly lower levels of 5-HT, 3-methoxytyramine (3-MT), 5-hydroxyindole-3-acetic acid (5-HIAA), homovanillic acid (HVA), and 3, 4-dihydroxyphenylacetic acid (DOPAC) in the cerebral cortex than those of the vehicle control-treated mice. The levels of HVA and 5-HIAA in the hippocampus were also significantly lower in the mice treated with compound 1 than in the control group mice. These results suggest that the metabolic changes, reflected in the levels of DA and/or 5-HT, may contribute to the anxiolytic activity of the phenanthrenoids studied herein.


Assuntos
Ansiolíticos/farmacologia , Córtex Cerebral/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Magnoliopsida/química , Fenantrenos/farmacologia , Ácido 3,4-Di-Hidroxifenilacético/análise , Animais , Ansiolíticos/isolamento & purificação , Córtex Cerebral/química , China , Dopamina/análogos & derivados , Dopamina/análise , Hipocampo/química , Ácido Homovanílico/análise , Masculino , Camundongos , Estrutura Molecular , Fenantrenos/isolamento & purificação , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Raízes de Plantas/química , Serotonina/análise
18.
Mikrochim Acta ; 186(1): 49, 2019 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-30610391

RESUMO

This review (with 131 references) summarizes the progress made in the past years in the field of nanomaterial based sensing of serotonin (5-HT). An introduction summarizes the significant role of 5-HT as a biomarker for several major diseases, methods for its determination and the various kinds of nanomaterials for use in electrochemical sensing process relies principally on a precise choice of electrodes. The next main section covers nanomaterial based methods for sensing 5-HT, with subsections on electrodes modified with carbon nanotubes, graphene related materials, gold nanomaterials, and by other nanomaterials. A concluding section discusses future perspectives and current challenges of 5-HT determination. Graphical abstract Conceptual design of electrochemical sensing process of the biomarker serotonin by using nanomaterials and the role of 5-HTas biomarker in the body from preclinical to clincal.


Assuntos
Técnicas Eletroquímicas/métodos , Nanoestruturas/química , Serotonina/análise , Animais , Biomarcadores/análise , Técnicas Eletroquímicas/tendências , Eletrodos/tendências , Humanos
19.
Adv Mater ; 31(6): e1805752, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30548684

RESUMO

A major difficulty in implementing carbon-based electrode arrays with high device-packing density is to ensure homogeneous and high sensitivities across the array. Overcoming this obstacle requires quantitative microscopic models that can accurately predict electrode sensitivity from its material structure. Such models are currently lacking. Here, it is shown that the sensitivity of graphene electrodes to dopamine and serotonin neurochemicals in fast-scan cyclic voltammetry measurements is strongly linked to point defects, whereas it is unaffected by line defects. Using the physics of point defects in graphene, a microscopic model is introduced that explains how point defects determine sensitivity. The predictions of this model match the empirical observation that sensitivity linearly increases with the density of point defects. This model is used to guide the nanoengineering of graphene structures for optimum sensitivity. This approach achieves reproducible fabrication of miniaturized sensors with extraordinarily higher sensitivity than conventional materials. These results lay the foundation for new integrated electrochemical sensor arrays based on nanoengineered graphene.


Assuntos
Dopamina/análise , Grafite/química , Serotonina/análise , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Eletrodos , Modelos Moleculares , Sensibilidade e Especificidade , Propriedades de Superfície
20.
Drug Res (Stuttg) ; 69(7): 392-400, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30296804

RESUMO

AIM: In the present study quercetin was studied for its role in inflammation, oxidative stress markers and 5-HT levels in unpredictable chronic mild stress (UCMS) animal model of depression. MATERIALS AND METHODS: The mice were randomized into different groups trained for UCMS protocol followed by different drug treatments. Treatments were started after 2 weeks from the start of UCMS protocol and continued up to 6 weeks. The behavioral tests such as modified forced swimming (MFST), tail suspension (TST) and open field tests were performed on week 6, at least 24 h after the last drug treatment. Behavioral tests were preceded following animal sacrifice for biochemical estimations. RESULTS: A significant decrease in swimming, climbing times and increase in immobility time in MFST and TST was observed in UCMS group. Administration of quercetin (25 mg/kg per orally (p.o) reversed these times in MFST and TST. A decrease in no. of field crossing, time spent in centre and no. of rearing were observed in UCMS group. Quercetin reduced these observations in open field test. There was a decrease in superoxide dismutase (SOD), glutathione (GSH), catalase and 5 HT levels in the brain tissue. Quercetin treatment significantly augmented SOD, GSH, catalase and 5 HT levels. Glutamate, TNF-α and IL-6 levels were increased in UCMS group while quercetin decreased these cytokines. CONCLUSION: Quercetin resulted antidepressant-like effect by its antioxidant, anti-inflammatory activities, reduced excitotoxicity and augmented 5 HT levels. This pointed out the usefulness of this phenolic compound as adjuvent drug along with other antidepressant drugs.


Assuntos
Antidepressivos/farmacologia , Encéfalo/efeitos dos fármacos , Depressão/tratamento farmacológico , Inflamação/tratamento farmacológico , Quercetina/farmacologia , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Antidepressivos/uso terapêutico , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Comportamento Animal/efeitos dos fármacos , Biomarcadores/análise , Biomarcadores/metabolismo , Encéfalo/imunologia , Encéfalo/patologia , Depressão/etiologia , Depressão/patologia , Modelos Animais de Doenças , Humanos , Inflamação/patologia , Masculino , Camundongos , Estresse Oxidativo/efeitos dos fármacos , Quercetina/uso terapêutico , Serotonina/análise , Serotonina/metabolismo , Estresse Psicológico/complicações , Resultado do Tratamento
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