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1.
Arch Virol ; 164(12): 3089-3093, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31595357

RESUMO

The phage vB_BthS-HD29phi infecting Bacillus thuringiensis strain HD29 was isolated and purified. The morphology of the phage showed that it belongs to the family Siphoviridae. The phage genome was 32,181 bp in length, comprised linear double-stranded DNA with an average G + C content of 34.9%, and exhibited low similarity to known phage genomes. Genomic and phylogenetic analysis revealed that vB_BthS-HD29phi is a novel phage. In total, 50 putative ORFs were predicted in the phage genome, and only 18 ORFs encoded proteins with known functions. This article reports the genome sequence of a new tailed phage and increases the known genetic diversity of tailed phages.


Assuntos
Fagos Bacilares/genética , Bacillus thuringiensis/virologia , Genoma Viral , Siphoviridae/genética , Fagos Bacilares/classificação , Fagos Bacilares/isolamento & purificação , Composição de Bases , Sequência de Bases , DNA Viral/genética , Variação Genética , Filogenia , Siphoviridae/classificação , Siphoviridae/isolamento & purificação , Sequenciamento Completo do Genoma
2.
Arch Virol ; 164(12): 3111-3113, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31531744

RESUMO

The complete genome of the novel phage vB_EcoS_PHB17, which infects Shiga-toxin-producing Escherichia coli, was sequenced, revealing a linear double-stranded DNA genome of 48,939 bp with 46% GC content and protruding 150-bp 5' cohesive termini. The genome contained 85 open reading frames, 28 of which were annotated with known functions. No tRNA-encoding genes were detected. Phylogenetic analysis suggested that phage PHB17 is a novel phage of family Siphoviridae.


Assuntos
Escherichia coli Shiga Toxigênica/virologia , Siphoviridae/classificação , Sequenciamento Completo do Genoma/métodos , Composição de Bases , Tamanho do Genoma , Genoma Viral , Fases de Leitura Aberta , Filogenia , Siphoviridae/genética , Siphoviridae/isolamento & purificação
3.
Arch Virol ; 164(12): 3115-3119, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31535209

RESUMO

A virulent phage, named ST20, infecting Escherichia coli O165:H8 was isolated from wastewater and subjected to genomic sequencing using the Illumina HiSeq system. Genomic analysis revealed that it contains double-stranded DNA, and its complete genome consists of 44,517 nucleotides with an average GC content of 50.81%. Morphological observations showed that phage ST20 belongs to the order Caudovirales and the family Siphoviridae due to its characteristic icosahedral capsid and a long noncontractile tail. This phage was further characterized by one-step growth curve analysis and measurement of its stability at 4 °C. The study has implications for the development of potential biocontrol agents.


Assuntos
Escherichia coli Shiga Toxigênica/virologia , Siphoviridae/classificação , Sequenciamento Completo do Genoma/métodos , Bacteriófagos/classificação , Bacteriófagos/genética , Bacteriófagos/isolamento & purificação , Bacteriófagos/patogenicidade , Composição de Bases , Tamanho do Genoma , Siphoviridae/genética , Siphoviridae/isolamento & purificação , Siphoviridae/patogenicidade , Virulência , Águas Residuárias/microbiologia
4.
Curr Microbiol ; 76(11): 1256-1263, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31372731

RESUMO

A novel Alteromonas phage JH01, with the host strain identified to be Alteromonas marina SW-47(T), was isolated from the Qingdao coast during the summer of 2017. Transmission electron microscopy analysis showed that phage JH01 can be categorized into the Siphoviridae family, with an icosahedral head of 62 ± 5 nm and a long contractile tail of 254 ± 10 nm. The bioinformatic analysis shows that this phage consists of a linear, double-stranded 46,500 bp DNA molecule with a GC content of 44.39%, and 58 ORFs with no tRNA genes. The ORFs are classified into four groups, including phage packaging, phage structure, DNA replication and regulation, and hypothetical protein. The phylogenetic tree, constructed using neighbor-joining analysis, shows that phage JH01 has altitudinal homology with some Vibrio and Pseudoalteromonas phage B8b. Comparative analysis reveals the high similarity between phage JH01 and phage B8b. Additionally, our study of phage JH01 provides useful information for further research on the interaction between Alteromonas phages and their hosts.


Assuntos
Alteromonas/virologia , Bacteriófagos/isolamento & purificação , Genoma Viral , Água do Mar/virologia , Siphoviridae/isolamento & purificação , Bacteriófagos/classificação , Bacteriófagos/genética , Sequência de Bases , China , DNA Viral , Filogenia , Siphoviridae/classificação , Siphoviridae/genética
5.
Arch Virol ; 164(10): 2637-2640, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31372754

RESUMO

A novel lytic Raoultella phage, RP180, was isolated and characterized. The RP180 genome has 44,851 base pairs and contains 65 putative genes, 35 of them encoding proteins whose functions were predicted based on sequence similarity to known proteins. The RP180 genome possesses a gene synteny typical of members of the subfamily Guernseyvirinae. Phylogenetic analysis of the RP180 genome and similar phage genomes revealed that phage RP180 is the first member of the genus Kagunavirus, subfamily Guernseyvirinae, that is specific for Raoultella sp. The genome of RP180 encodes a putative protein with similarity to CRISPR-like Cas4 nucleases, which belong to the pfam12705/PDDEXK_1 family. Cas4-like proteins of this family have been shown to interfere with the bacterial host type II-C CRISPR-Cas system.


Assuntos
Bacteriófagos/classificação , Bacteriófagos/isolamento & purificação , Enterobacteriaceae/virologia , Filogenia , Siphoviridae/classificação , Siphoviridae/isolamento & purificação , Bacteriólise , Bacteriófagos/genética , Genoma Viral , Microscopia Eletrônica de Transmissão , Análise de Sequência de DNA , Siphoviridae/genética , Sintenia , Proteínas Virais/genética , Vírion/ultraestrutura
6.
Arch Virol ; 164(11): 2877-2880, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31451964

RESUMO

A temperate bacteriophage, IME1320_01, was induced by mitomycin C treatment from Corynebacterium striatum. This phage possesses a double-stranded DNA genome of 40,086 bp with a G+C content of 58%. A total of 53 putative open reading frames (ORFs) were identified in its genome. BLASTn analysis revealed that IME1320_01 had the highest sequence similarity to Corynebacterium striatum strain 216, with a genome homology coverage of 44% and highest sequence identity of 95%. The termini of the phage genome was non-redundant, with a 13-nt 3'-protruding cohesive end. To the best of our knowledge, phage IME1320_01 is the first inducible phage to be identified in Corynebacterium striatum.


Assuntos
Corynebacterium/virologia , Genoma Viral/genética , Siphoviridae/genética , Ativação Viral/efeitos dos fármacos , Composição de Bases/genética , DNA Viral/genética , Mitomicina/farmacologia , Fases de Leitura Aberta/genética , Análise de Sequência de DNA , Siphoviridae/classificação , Siphoviridae/isolamento & purificação
7.
Microbiol Res ; 228: 126300, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31422230

RESUMO

Two morphologically different bacteriophages were isolated from the river and soil samples from various locations of Maharashtra, India against the phytopathogen Pseudomonas sp. that was recently reported to cause a new bacterial blight of pomegranate. Both the phages belonged to the order Caudovirales representing the families Siphoviridae (vB_Psp.S_PRɸL2) and Myoviridae (vB_Psp.M_SSɸL8). The multiplicity of infection ranged from 0.01 to 0.1, phage adsorption rate from 39% to 66%, latent period from 10 to 20 min with a burst size of 24-85 phage particles per infected host cell. The genome size of phages PRɸL2 and SSɸL8 was approximately 25.403 kb and 29.877 kb respectively. Restriction digestion pattern of phage genomic DNA was carried out for phage PRɸL2, Eco RI resulted in two bands and Hind III resulted in three bands while for phage SSɸL8, both Eco RI and Hind III each resulted in three bands. SDS-PAGE protein profile showed six bands for PRɸL2 and nine bands for SSɸL8 of different proteins. Phages showed high pH stability over a range of 4-9, temperature stability over a range of 4-50 °C and UV radiation showed a reduction up to 89.36% for PRɸL2 and 96% for SSɸL8. In short, the present research work discusses for the first time in-detailed characterization of phages of a phytopathogen Pseudomonas sp. from Maharashtra, India, which can be further efficiently used for biological control of the causative agent of a new bacterial blight disease of pomegranate.


Assuntos
Lythraceae/microbiologia , Doenças das Plantas/microbiologia , Fagos de Pseudomonas/classificação , Fagos de Pseudomonas/isolamento & purificação , Pseudomonas/virologia , Caudovirales/classificação , Caudovirales/genética , Caudovirales/isolamento & purificação , Caudovirales/ultraestrutura , DNA Viral/análise , Especificidade de Hospedeiro , Concentração de Íons de Hidrogênio , Índia , Viabilidade Microbiana , Myoviridae/classificação , Myoviridae/genética , Myoviridae/isolamento & purificação , Myoviridae/ultraestrutura , Fagos de Pseudomonas/genética , Fagos de Pseudomonas/ultraestrutura , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/isolamento & purificação , Siphoviridae/ultraestrutura , Temperatura Ambiente , Raios Ultravioleta/efeitos adversos , Proteínas Virais/análise
8.
Arch Virol ; 164(9): 2277-2284, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31222428

RESUMO

To gain insight into the presence and nature of prophages in the black soldier fly (BSF; Hermetia illucens L. [Diptera: Stratiomyidae]) gut, we isolated and characterized a novel, temperate Escherichia bacteriophage designated vB_EcoS_PHB10 (PHB10). Electron microscopy analysis revealed that phage PHB10 has a long, flexible, non-contractile tail and belongs to the family Siphoviridae. The phage was found to be stable over a wide range of temperatures (4-37 °C) and pH values (pH 5-9), and it lysed two out of 13 Escherichia strains tested. The genome of PHB10 contains genes encoding a putative transcriptional regulator and an integrase, and it shows a high degree of similarity to a region of the Enterobacter cloacae MBRL1077 genome. Induction experiments revealed that phage PHB10 could be induced by different gut substrates, suggesting that diet might be a potential regulator of lytic/lysogenic switches in commensal lysogens.


Assuntos
Bacteriófagos/isolamento & purificação , Escherichia/virologia , Intestinos/virologia , Simuliidae/microbiologia , Simuliidae/virologia , Siphoviridae/isolamento & purificação , Animais , Bacteriófagos/classificação , Bacteriófagos/genética , Bacteriófagos/fisiologia , Genoma Viral , Intestinos/microbiologia , Larva/microbiologia , Larva/virologia , Lisogenia , Filogenia , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/fisiologia
9.
BMC Res Notes ; 12(1): 223, 2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-30975220

RESUMO

OBJECTIVE: Zero-valent iron sand filtration can remove multiple contaminants, including some types of pathogenic bacteria, from contaminated water. However, its efficacy at removing complex viral populations, such as those found in reclaimed water used for agricultural irrigation, has not been fully evaluated. Therefore, this study utilized metagenomic sequencing and epifluorescent microscopy to enumerate and characterize viral populations found in reclaimed water and zero-valent iron-sand filtered reclaimed water sampled three times during a larger greenhouse study. RESULTS: Zero-valent iron-sand filtered reclaimed water samples had significantly less virus-like particles than reclaimed water samples at all collection dates, with the reclaimed water averaging between 108 and 109 and the zero-valent iron-sand filtered reclaimed water averaging between 106 and 107 virus-like particles per mL. In addition, for both sample types, viral metagenomes (viromes) were dominated by bacteriophages of the order Caudovirales, largely Siphoviridae, and genes related to DNA metabolism. However, the proportion of sequences homologous to bacteria, as well as the abundance of genes possibly originating from a bacterial host, was higher in the viromes of zero-valent iron-sand filtered reclaimed water samples. Overall, zero-valent iron-sand filtered reclaimed water had a lower total concentration of virus-like particles and a different virome community composition compared to unfiltered reclaimed water.


Assuntos
Bactérias/genética , Caudovirales/genética , Recuperação e Remediação Ambiental/métodos , Ferro/química , Dióxido de Silício/química , Siphoviridae/genética , Adsorção , Irrigação Agrícola/métodos , Bactérias/classificação , Bactérias/isolamento & purificação , Caudovirales/classificação , Caudovirales/isolamento & purificação , DNA Bacteriano/genética , DNA Viral/genética , Filtração/métodos , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Metagenômica/métodos , Filogenia , Siphoviridae/classificação , Siphoviridae/isolamento & purificação , Vírion/isolamento & purificação , Águas Residuárias/microbiologia , Águas Residuárias/virologia , Purificação da Água/métodos
10.
Microb Pathog ; 131: 175-180, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30946984

RESUMO

BACKGROUND: Shigella dysenteriae is one of the members of Shigella genus which was the main responsible of different Shigellosis outbreaks worldwide. The increasing consumption of antibiotics has led to the emergence and spreading of antibiotic-resistant strains. Therefore, finding new alternatives for infection control is essential, one of which is using bacteriophages. MATERIALS AND METHODS: Lytic bacteriophage against Shigella dysenteriae was isolated from petroleum refinery wastewater. Phage morphological and genetic characteristics were studied using TEM, and sequencing, respectively. In addition, the genome size was estimated, and phage resistance to different temperatures and pH, host range, adsorption rate, and one-step growth were investigated. RESULTS: According to the morphology and genetic results, this phage was named vB-SdyS-ISF003. Sequencing of the PCR products revealed that the vB-SdyS-ISF003 phage belongs to the species T1virus, subfamily Tunavirinae of family Siphoviridae. This was the first detected bacteriophage against S. dysenteriae, which belongs to the family Siphoviridae. In addition, its host range was limited to S. dysenteriae. The genome size was about 62 kb. vB-SdyS-ISF003 phage has a number of desirable characteristics including the limited host range to S. dysenteriae, very short connection time, a relatively wide range of temperature tolerance -20 to 50 °C, pH tolerance of 7-9 without significant reduction in the phage titer. CONCLUSION: vB-SdyS-ISF003 is a novel virulent T1virus phage and has the appropriate potential for being used in bio controlling of S. dysenteriae in different condition.


Assuntos
Reação em Cadeia da Polimerase/métodos , Shigella dysenteriae/virologia , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/isolamento & purificação , DNA Viral/genética , Tamanho do Genoma , Genoma Viral , Especificidade de Hospedeiro , Concentração de Íons de Hidrogênio , Terapia por Fagos , Shigella dysenteriae/patogenicidade , Siphoviridae/crescimento & desenvolvimento , Temperatura Ambiente , Termotolerância
11.
Virus Genes ; 55(3): 381-393, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30927185

RESUMO

Bacteriophages often constitute the majority of periodontal viral communities, but phages that infect oral bacteria remain uncharacterized. Here, we present the genetic analysis of the genome of a novel siphovirus, named Siphoviridae_29632, which was isolated from a patient with periodontitis using a viral metagenomics-based approach. Among 43 predicted open reading frames (ORFs) in the genome, the viral genes encoding structural proteins were distinct from the counterparts of other viruses, although a distant homology is shared among viral morphogenesis proteins. A total of 28 predicted coding sequences had significant homology to other known phage ORF sequences. In addition, the prevalence of Siphoviridae_29632 in a cohort of patients with chronic periodontitis was 41.67%, which was significantly higher than that in the healthy group (4.55%, P < 0.001), suggesting that this virus as well as its hosts may contribute to the ecological environment favored for chronic periodontitis.


Assuntos
Bacteriófagos/genética , Periodontite Crônica/virologia , Bolsa Periodontal/virologia , Siphoviridae/genética , Bacteriófagos/isolamento & purificação , Bacteriófagos/patogenicidade , Periodontite Crônica/genética , Periodontite Crônica/microbiologia , Genoma Viral/genética , Genômica , Humanos , Metagenômica , Bolsa Periodontal/genética , Bolsa Periodontal/microbiologia , Filogenia , Siphoviridae/isolamento & purificação
12.
Curr Microbiol ; 76(5): 558-565, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30810780

RESUMO

Despite their ubiquity, relatively few bacteriophages have been characterized. Here, we set out to explore Caulobacter bacteriophages (caulophages) in the rhizosphere and characterized Kronos, the first caulophage isolated from the rhizosphere. Kronos is a member of the Siphoviridae family since it has a long flexible tail. In addition, an analysis of the Kronos genome indicated that many of the predicted proteins were distantly related to those of bacteriophages in the lambdoid family. Consistent with this observation, we were able to demonstrate the presence of cos sites that are similar to those found at the ends of lambdoid phage genomes. Moreover, Kronos displayed a relatively rare head and tail morphology compared to other caulophages but was similar to that of the lambdoid phages. Taken together, these data indicate that Kronos is distantly related to lambdoid phages and may represent a new Siphoviridae genus.


Assuntos
Caulobacter/virologia , Rizosfera , Siphoviridae/isolamento & purificação , Bacteriófago lambda/genética , Genoma Viral , Siphoviridae/classificação
13.
Arch Virol ; 164(3): 879-884, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30506471

RESUMO

Escherichia coli bacteriophage Gostya9 (genus T5virus) was isolated from horse feces collected in Moscow, Russia, in 2013. This phage was associated in a single plaque with the previously reported phage 9g and was subsequently purified. Analysis of the complete genomic sequence of Gostya9 revealed that it is closely related to the T5-like bacteriophage DT57C, which had been isolated at the same location in 2007. These two viruses share 79.5% nucleotide sequence identity, which is below the 95% threshold applied currently to demarcate bacteriophage species. The most significant features distinguishing Gostya9 from DT57C include 1) the presence of one long tail fiber protein gene, 122c (ltf), instead of the two genes, ltfA and ltfB, that are present in DT57C; 2) the absence of the gene for the receptor-blocking lytic conversion lipoprotein precursor llp; and 3) the divergence of the receptor-recognition protein, pb5, which is only distantly related at the amino acid sequence level. The observed features of the Gostya9 adsorption apparatus are suggestive of a possible novel specificity for the final receptor and make this phage interesting for possible direct application in phage therapy of E. coli infections or as a source of receptor-recognition protein for engineering new phage specificities.


Assuntos
Colífagos/isolamento & purificação , Escherichia coli/virologia , Siphoviridae/isolamento & purificação , Animais , Colífagos/classificação , Colífagos/genética , Colífagos/ultraestrutura , Escherichia coli/genética , Escherichia coli/metabolismo , Fezes/virologia , Genoma Viral , Cavalos , Receptores Virais/genética , Receptores Virais/metabolismo , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/ultraestrutura , Proteínas Virais/genética , Proteínas Virais/metabolismo
14.
Arch Virol ; 164(3): 667-674, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30523430

RESUMO

Viruses are ubiquitous in the biosphere and greatly affect the hosts they infect. It is generally accepted that members of every microbial taxon are susceptible to at least one virus, and a plethora of bacterial viruses are known. In contrast, knowledge of the archaeal virosphere is still limited. Here, a novel lytic archaeal virus is described, designated "Drs3", as well as its host, Methanobacterium formicicum strain Khl10. This hydrogenotrophic methanogenic archaeon and its virus were isolated from the anaerobic digester of an experimental biogas plant in Germany. The tailed virus has an icosahedral head with a diameter of approximately 60 nm and a long non-contractile tail of approximately 230 nm. These structural observations suggest that the new isolate belongs to the family Siphoviridae, but it could not be assigned to an existing genus. Lysis of the host Khl10 was observed 40-44 h after infection. Lysis of the type strain Methanobacterium formicicum DSMZ 1535 was not observed in the presence of Drs3, pointing towards resistance in the type strain or a rather narrow host range of this newly isolated archaeal virus. The complete 37-kb linear dsDNA genome of Drs3 contains 39 open reading frames, only 12 of which show similarity to genes with predicted functions.


Assuntos
Vírus de Archaea/isolamento & purificação , Methanobacterium/virologia , Siphoviridae/isolamento & purificação , Vírus de Archaea/classificação , Vírus de Archaea/genética , Vírus de Archaea/fisiologia , Alemanha , Especificidade de Hospedeiro , Fases de Leitura Aberta , Filogenia , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/fisiologia , Proteínas Virais/genética
15.
Folia Microbiol (Praha) ; 64(3): 283-294, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30284669

RESUMO

Antibiotic resistance is increasing among Staphylococcus saprophyticus strains isolated from urinary tract infection. This necessitates alternative therapies. For this, a lytic phage (vB_SsapS-104) against S. saprophyticus, which formed round and clear plaques on bacterial culture plates, was isolated from hospital wastewater and characterized. Microscopy analysis showed that it had a small head (about 50 nm), tail (about 80 nm), and a collar (about 22 nm in length and 19 nm in width) indicating to be a phage within Siphoviridae family. Phage vB_SsapS-104 showed a large latency period of about 40 min, rapid adsorption rate that was significantly enhanced by MgCl2 and CaCl2, and high stability to a wide range of temperatures and pH values. Restriction analyses demonstrated that phage consists of a double-stranded DNA with an approximate genome size of 40 Kb. BLAST results did not show high similarity (megablast) with other previously identified phages. But, in Blastn, similarity with Staphylococcus phages was observed. Phage vB_SsapS-104 represented high anti-bacterial activity against S. saprophyticus isolates in vitro as it was able to lyse 8 of the 9 clinical isolates (%88.8) obtained from a hospital in Gorgan, Iran. It was a S. saprophyticus-specific phage because no lytic activity was observed on some other pathogenic bacteria tested. Therefore, phage vB_SsapS-104 can be considered as a specific virulent phage against of S. saprophyitcus isolated from urinary tract infection. This study provided the partial genomic characterization of S. saprophyticus phage and its application against urinary tract infection associated with S. saprophyticus. This phage also can be considered as a good candidate for a therapeutic alternative in the future.


Assuntos
Siphoviridae/genética , Siphoviridae/isolamento & purificação , Staphylococcus saprophyticus/virologia , Infecções Urinárias/microbiologia , Antibacterianos/farmacologia , DNA Viral , Genoma Viral , Especificidade de Hospedeiro , Humanos , Concentração de Íons de Hidrogênio , Irã (Geográfico) , Microscopia Eletrônica de Transmissão , Terapia por Fagos , Análise de Sequência de DNA , Siphoviridae/ultraestrutura , Infecções Estafilocócicas/microbiologia , Fagos de Staphylococcus/genética , Staphylococcus saprophyticus/efeitos dos fármacos , Temperatura Ambiente , Virulência , Latência Viral , Águas Residuárias/virologia
16.
Res Microbiol ; 170(1): 13-23, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30077624

RESUMO

Vibrio parahaemolyticus is a human enteropathogenic bacterium and is also pathogenic to shrimp and finfish. In a search for a biocontrol agent for V. parahaemolyticus and other pathogenic Vibrio species, a lytic phage VP06 was isolated from oyster using V. parahaemolyticus as the host. VP06 is a Siphoviridae phage with a polyhedral head and a long tail. The genome sequence of VP06 was 75,893 nucleotides in length and the G + C content was 49%; a total of 101 CDSs were identified in VP06, of which 39 exhibited functional domains/motifs. The genomic sequence of VP06 is similar to those of a lytic Vibrio vulnificus phage SSP002 and a temperate V. parahaemolyticus phage vB_VpaS_MAR10, although VP06 has distinct features in the CDS arrangement and 14 unique CDSs. Phylogenetic analysis revealed that VP06, SSP002 and vB_VpaS_MAR10 belong to a novel genus cluster of Siphoviridae phages. This phage lysed 28.1% of various Vibrio strains, and the efficiency of plating method revealed that VP06 was highly effective in lysing strains of Vibrio alginolyticus, Vibrio azureus, Vibrio harveyi and V. parahaemolyticus. The properties of VP06, including its broad range of hosts and resistance to environmental stresses, indicate that it may be a candidate biocontrol agent.


Assuntos
Bacteriófagos/metabolismo , Siphoviridae/isolamento & purificação , Vibrio parahaemolyticus/virologia , Vírus/isolamento & purificação , Animais , Bacteriófagos/genética , Composição de Bases , Genoma Viral , Ostreidae/virologia , Filogenia , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/ultraestrutura , Vírus/classificação , Vírus/genética , Vírus/ultraestrutura
17.
Int J Food Microbiol ; 292: 107-117, 2019 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-30594742

RESUMO

The growth of Shewanella spp., mainly S. baltica and S. putrefaciens, is responsible for the spoilage of chilled fresh fish. Phages are an alternative tool to control bacterial growth. In this study, virulent phages infecting 4 S. baltica and 6 S. putrefaciens strains were isolated and characterized. Transmission electron microscopy revealed that 6 out of 10 phages (3 phages infecting S. baltica and 3 phages infecting S. putrefaciens) belonged to Myoviridae, while the other 4 phages (1 phage infecting S. baltica and 3 phages infecting S. putrefaciens) belonged to Siphoviridae. Phage SppYZU01 and SppYZU05 showed the broadest host range, being lytic towards all the 4 S. baltica strains and 5 out of the 6 S. putrefaciens strains, respectively. The genome sequence of SppYZU01 had no similarity with known genome sequences, while that of SppYZU05 was 88.5% similar to the genome of a virulent S. putrefaciens-infecting phage (Spp001). According to the host range and lytic activity, 3 phages, including SppYZU01, SppYZU05, and SppYZU06, were combined into a cocktail (designated as SPMIX3-156). SPMIX3-156 showed potential as an antimicrobial agent to control S. baltica and S. putrefaciens strain growth in catfish matrices. Bacterial growth in the catfish muscle juice inoculated with 104 colony-forming units (CFU)/mL of Shewanella strains was partially inhibited by 105 plaque-forming units (PFU)/mL of SPMIX3-156 at both 25 °C and 4 °C. The catfish fillets inoculated with Shewanella strains were used as a model to evaluate the biopreservative effects of SPMIX3-156. Total viable counts of fillet samples treated with 107 PFU/mL of SPMIX3-156 were reduced by 3.21 and 2.75 log units after 1 day at 25 °C and 10 day at 4 °C, respectively, compared to those of untreated samples. Fillet quality indices, including pH, total volatile basic nitrogen, and sensory value of the SPMIX3-156-treated samples, considerably improved compared to those of the control samples at both 4 °C and 25 °C. Our results suggest that SPMIX3-156 is a promising biological agent against S. baltica and S. putrefaciens, and may have a potential use in chilled fish fillet biopreservation.


Assuntos
Bacteriófagos/genética , Genes Virais , Ictaluridae/microbiologia , Shewanella putrefaciens/virologia , Shewanella/virologia , Animais , Bacteriófagos/isolamento & purificação , Agentes de Controle Biológico , Contagem de Colônia Microbiana , DNA Viral/isolamento & purificação , Contaminação de Alimentos , Microbiologia de Alimentos , Conservação de Alimentos , Myoviridae/isolamento & purificação , Alimentos Marinhos/microbiologia , Análise de Sequência de DNA , Siphoviridae/isolamento & purificação
18.
Curr Microbiol ; 75(12): 1619-1625, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30218176

RESUMO

We used the double-agar layer method to isolate a novel Marinobacter marina bacteriophage, B23, from the surface water sample of the Bohai sea of China. There is some work to better understand the phage. The result of transmission electron microscopy revealed that B23 belongs to the family Siphoviridae with a head of 80 nm in diameter and a tail of 230 nm. Microbiological characterization evidenced that phage B23 is stable at the temperatures from - 25 to 60 °C, and showed vigorous vitality at pH between 4.0 and 12.0. One-step growth experiment showed that it had a longer latent period and higher lysis efficiency. Furthermore, the complete genome of B23 was sequenced and analyzed, which consists of a 35132 bp DNA with a G + C content of 59.8% and 50 putative open reading frames. The genome was divided into five parts, consisting of DNA replication and regulation, phage packaging, phage structure, host lysis and hypothetical protein.


Assuntos
Genoma Viral , Marinobacter/virologia , Siphoviridae/genética , Concentração de Íons de Hidrogênio , Filogenia , Siphoviridae/crescimento & desenvolvimento , Siphoviridae/isolamento & purificação , Siphoviridae/ultraestrutura , Temperatura Ambiente , Sequenciamento Completo do Genoma
19.
J Gen Virol ; 99(10): 1453-1462, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30102145

RESUMO

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a serious bacterial disease in rice-growing regions worldwide. Phage therapy has been proposed as a potential measure to treat bacterial infections. In this study, a novel phage, Xoo-sp2, which infects Xoo was isolated from soil. The characteristics of Xoo-sp2, including the morphology, one-step growth curve and host range, were analysed. The genome of phage Xoo-sp2 was sequenced and annotated. The results demonstrated that Xoo-sp2 is a siphovirus and has a broad lytic spectrum, infecting 9 out of 10 representative Xoo strains. Genome analysis showed that the Xoo-sp2 genome consists of a linear double-stranded DNA molecule of length 60 370 bp. Annotation of the whole genome indicated that Xoo-sp2 encodes 79 putative open reading frames (ORFs). Comparative genomics analysis of Xoo-sp2 showed that it shares significant similarity only with Pseudomonas and Stenotrophomonas phages (with maximum identity reaching 80 % along 69 % of the genome), and thus represents a novel Xanthomonas phage. Xoo-sp2 significantly inhibited Xoo growth in liquid culture. An experiment with potted plants indicated that Xoo-sp2 could efficiently control BLB in living rice. In summary, our work characterized a novel Xanthomonas phage and demonstrated its potential as a prophylactic agent in the control of BLB in rice.


Assuntos
Bacteriófagos/classificação , Bacteriófagos/isolamento & purificação , Siphoviridae/classificação , Siphoviridae/isolamento & purificação , Xanthomonas/virologia , Bacteriófagos/genética , Bacteriófagos/crescimento & desenvolvimento , DNA/química , DNA/genética , DNA Viral/química , DNA Viral/genética , Genoma Viral , Especificidade de Hospedeiro , Anotação de Sequência Molecular , Fases de Leitura Aberta , Oryza/microbiologia , Doenças das Plantas/microbiologia , Análise de Sequência de DNA , Homologia de Sequência , Siphoviridae/genética , Siphoviridae/crescimento & desenvolvimento , Microbiologia do Solo , Xanthomonas/crescimento & desenvolvimento
20.
Arch Virol ; 163(11): 3195-3197, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30094514

RESUMO

AsXd-1, a bacteriophage that infects Aeromonas salmonicida, was isolated from the wastewater of a seafood market in Shenzhen, China. The 39,014-bp genome of this phage contains 52 open reading frames (ORFs), 30 of which were found to be homologous to reference sequences that putatively encode functional phage proteins. Nine out of the remaining 22 ORFs with unknown functions were unique to AsXd-1. Gene annotation suggests that AsXd-1 has both lysogenic and lytic life cycles. Furthermore, both phylogenetic analysis based on the large subunit of terminase and genome sequence comparisons show that AsXd-1 is closely related to phages belonging to the genus Hk97virus. We thus propose AsXd-1 as a new member of the genus Hk97virus within the family Siphoviridae.


Assuntos
Aeromonas/virologia , Bacteriófagos/genética , Bacteriófagos/isolamento & purificação , Genoma Viral , Siphoviridae/classificação , Siphoviridae/isolamento & purificação , Bacteriófagos/classificação , Sequência de Bases , China , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Análise de Sequência de DNA , Siphoviridae/genética , Proteínas Virais/genética
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