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1.
Sensors (Basel) ; 21(12)2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34207808

RESUMO

A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care.


Assuntos
Técnicas Biossensoriais , MicroRNAs , Desenho de Equipamento , Limite de Detecção , Sistemas Automatizados de Assistência Junto ao Leito
2.
Praxis (Bern 1994) ; 110(9): 488-507, 2021 Jul.
Artigo em Alemão | MEDLINE | ID: mdl-34231384

RESUMO

CME Sonography 100: Emergency Ultrasound of the Soft Tissues and the Musculoskeletal System Abstract. The term "emergency sonography" refers to a focused sonography in emergency situations, also called emergency "Point of Care Ultrasound (POCUS)". The attending physician applies it specifically and directly to the patient. As an indispensable part of the physical examination, emergency ultrasound helps to answer simple clinical questions. The corresponding answers provide essential elements for diagnostic and therapeutic decision-making. Furthermore, the emergency ultrasound increases the safety and efficiency of interventions on the musculoskeletal system and soft tissues. In this article we will discuss common clinical emergency situations in a focused way, but we will not address the regional anesthesiologic and analgesia-related applications that are also important in this context.


Assuntos
Sistema Musculoesquelético , Serviço Hospitalar de Emergência , Humanos , Sistema Musculoesquelético/diagnóstico por imagem , Dor , Exame Físico , Sistemas Automatizados de Assistência Junto ao Leito , Ultrassonografia
3.
Emerg Med Clin North Am ; 39(3): 509-527, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34215400

RESUMO

Point-of-care ultrasound has become an essential part of pediatric emergency medicine training and practice. It can have significant clinical benefits, including improving diagnostic accuracy and decreasing length of stay, and does not require radiation exposure for patients. In this review, we summarize the current diagnostic point-of-care ultrasound applications in pediatric emergency medicine, their evidence, and techniques.


Assuntos
Medicina de Emergência Pediátrica , Sistemas Automatizados de Assistência Junto ao Leito , Ultrassonografia , Doenças Biliares/diagnóstico por imagem , Criança , Oftalmopatias/diagnóstico por imagem , Feminino , Avaliação Sonográfica Focada no Trauma , Doenças da Vesícula Biliar/diagnóstico por imagem , Gastroenteropatias/diagnóstico por imagem , Doenças dos Genitais Femininos/diagnóstico por imagem , Cardiopatias/diagnóstico por imagem , Humanos , Nefropatias/diagnóstico por imagem , Pneumopatias/diagnóstico por imagem , Masculino , Gravidez , Torção do Cordão Espermático/diagnóstico por imagem , Doenças da Bexiga Urinária/diagnóstico por imagem , Veia Cava Inferior/diagnóstico por imagem
4.
Emerg Med Clin North Am ; 39(3): 529-554, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34215401

RESUMO

Point-of-care ultrasound can improve efficacy and safety of pediatric procedures performed in the emergency department. This article reviews ultrasound guidance for the following pediatric emergency medicine procedures: soft tissue (abscess incision and drainage, foreign body identification and removal, and peritonsillar abscess drainage), musculoskeletal and neurologic (hip arthrocentesis, peripheral nerve blocks, and lumbar puncture), vascular access (peripheral intravenous access and central line placement), and critical care (endotracheal tube placement, pericardiocentesis, thoracentesis, and paracentesis). By incorporating ultrasound, emergency physicians caring for pediatric patients have the potential to enhance their procedural scope, confidence, safety, and success.


Assuntos
Medicina de Emergência Pediátrica , Sistemas Automatizados de Assistência Junto ao Leito , Ultrassonografia de Intervenção , Abscesso/diagnóstico por imagem , Abscesso/cirurgia , Artrocentese/métodos , Cateterismo Venoso Central/métodos , Cateterismo Periférico/métodos , Criança , Drenagem/métodos , Corpos Estranhos/diagnóstico por imagem , Corpos Estranhos/cirurgia , Articulação do Quadril/diagnóstico por imagem , Humanos , Intubação Intratraqueal/métodos , Bloqueio Nervoso/métodos , Paracentese/métodos , Nervos Periféricos/diagnóstico por imagem , Abscesso Peritonsilar/diagnóstico por imagem , Abscesso Peritonsilar/terapia , Punção Espinal/métodos
5.
Sensors (Basel) ; 21(13)2021 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-34202483

RESUMO

Since the introduction of paper-based analytical devices as potential diagnostic platforms a few decades ago, huge efforts have been made in this field to develop systems suitable for meeting the requirements for the point-of-care (POC) approach. Considerable progress has been achieved in the adaptation of existing analysis methods to a paper-based format, especially considering the chemiluminescent (CL)-immunoassays-based techniques. The implementation of biospecific assays with CL detection and paper-based technology represents an ideal solution for the development of portable analytical devices for on-site applications, since the peculiarities of these features create a unique combination for fitting the POC purposes. Despite this, the scientific production is not paralleled by the diffusion of such devices into everyday life. This review aims to highlight the open issues that are responsible for this discrepancy and to find the aspects that require a focused and targeted research to make these methods really applicable in routine analysis.


Assuntos
Técnicas Biossensoriais , Luminescência , Imunoensaio , Sistemas Automatizados de Assistência Junto ao Leito
6.
Glob Heart ; 16(1): 42, 2021 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-34211828

RESUMO

Background: QTc prolongation is an adverse effect of COVID-19 therapies. The use of a handheld device in this scenario has not been addressed. Objectives: To evaluate the feasibility of QTc monitoring with a smart device in COVID-19 patients receiving QTc-interfering therapies. Methods: Prospective study of consecutive COVID-19 patients treated with hydroxychloroquine ± azithromycin ± lopinavir-ritonavir. ECG monitoring was performed with 12-lead ECG or with KardiaMobile-6L. Both registries were also sequentially obtained in a cohort of healthy patients. We evaluated differences in QTc in COVID-19 patients between three different monitoring strategies: 12-lead ECG at baseline and follow-up (A), 12-lead ECG at baseline and follow-up with the smart device (B), and fully monitored with handheld 6-lead ECG (group C). Time needed to obtain an ECG registry was also documented. Results: One hundred and eighty-two COVID-19 patients were included (A: 119(65.4%); B: 50(27.5%); C: 13(7.1%). QTc peak during hospitalization did significantly increase in all groups. No differences were observed between the three monitoring strategies in QTc prolongation (p = 0.864). In the control group, all but one ECG registry with the smart device allowed QTc measurement and mean QTc did not differ between both techniques (p = 0.612), displaying a moderate reliability (ICC 0.56 [0.19-0.76]). Time of ECG registry was significantly longer for the 12-lead ECG than for handheld device in both cohorts (p < 0.001). Conclusion: QTc monitoring with KardiaMobile-6L in COVID-19 patients was feasible. Time of ECG registration was significantly lower with the smart device, which may offer an important advantage for prevention of virus dissemination among healthcare providers.


Assuntos
COVID-19/tratamento farmacológico , Eletrocardiografia/métodos , Síndrome do QT Longo/diagnóstico , Idoso , Idoso de 80 Anos ou mais , Antibacterianos/efeitos adversos , Antivirais/efeitos adversos , Azitromicina/efeitos adversos , Combinação de Medicamentos , Eletrocardiografia/instrumentação , Inibidores Enzimáticos/efeitos adversos , Estudos de Viabilidade , Feminino , Humanos , Hidroxicloroquina/efeitos adversos , Síndrome do QT Longo/induzido quimicamente , Lopinavir/efeitos adversos , Masculino , Pessoa de Meia-Idade , Sistemas Automatizados de Assistência Junto ao Leito , Estudos Prospectivos , Reprodutibilidade dos Testes , Ritonavir/efeitos adversos , SARS-CoV-2
7.
Rev Med Suisse ; 17(743): 1183-1185, 2021 Jun 16.
Artigo em Francês | MEDLINE | ID: mdl-34133097

RESUMO

Point-of-care ultrasound is a diagnostic tool that is gaining increasingly more ground in general and emergency practice; it allows the clinician to answer certain precise questions, including the presence of a small bowel occlusion. For this indication, ultrasound is useful in rapidly establishing a diagnosis and planning further work up.


Assuntos
Serviço Hospitalar de Emergência , Sistemas Automatizados de Assistência Junto ao Leito , Emergências , Humanos , Atenção Primária à Saúde , Ultrassonografia
8.
Int J Mol Sci ; 22(9)2021 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-34063629

RESUMO

Hepatitis B remains a major global public health challenge, with particularly high prevalence in medically disadvantaged western Pacific and African regions. Although clinically available technologies for the qPCR detection of HBV are well established, research on point-of-care testing has not progressed substantially. The development of a rapid, accurate point-of-care test is essential for the prevention and control of hepatitis B in medically disadvantaged rural areas. The development of the CRISPR/Cas system in nucleic acid detection has allowed for pathogen point-of-care detection. Here, we developed a rapid and accurate point-of-care assay for HBV based on LAMP-Cas12a. It innovatively solves the problem of point-of-care testing in 10 min, particularly the problem of sample nucleic acid extraction. Based on LAMP-Cas12a, visualization of the assay results is presented by both a fluorescent readout and by lateral flow test strips. The lateral flow test strip technology can achieve results visible to the naked eye, while fluorescence readout can achieve real-time high-sensitivity detection. The fluorescent readout-based Cas12a assay can achieve HBV detection with a limit of detection of 1 copy/µL within 13 min, while the lateral flow test strip technique only takes 20 min. In the evaluation of 73 clinical samples, the sensitivity and specificity of both the fluorescence readout and lateral flow test strip method were 100%, and the results of the assay were fully comparable to qPCR. The LAMP-Cas12a-based HBV assay relies on minimal equipment to provide rapid, accurate test results and low costs, providing significant practical value for point-of-care HBV detection.


Assuntos
Técnicas Biossensoriais , Vírus da Hepatite B/isolamento & purificação , Hepatite B/diagnóstico , Testes Imediatos , Sistemas CRISPR-Cas/genética , Fluorescência , Hepatite B/genética , Hepatite B/virologia , Vírus da Hepatite B/patogenicidade , Humanos , Sistemas Automatizados de Assistência Junto ao Leito
9.
J Vet Diagn Invest ; 33(4): 695-702, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34075827

RESUMO

Previous studies have determined that, compared to whole blood, serum or plasma used in a portable blood glucometer (PBG) may provide more accurate results. We investigated the accuracy of a veterinary PBG (AlphaTRAK 2; Zoetis) for the measurement of glucose concentrations in serum, plasma, and whole blood compared to plasma glucose concentration measured by a biochemical analyzer. Blood samples from 53 client-owned dogs were collected. Lin concordance correlation coefficient (ρc) and Bland-Altman plots were used to determine correlation and agreement between the results obtained for the different sample types. Glucose concentration in whole blood measured by the veterinary PBG was more strongly correlated with the glucose concentration measured by the biochemical analyzer (ρc = 0.92) compared to plasma and serum glucose concentrations (ρc = 0.59 and 0.57, respectively). The mean differences between the glucose concentrations in whole blood, plasma, and serum measured by the veterinary PBG and the glucose concentration determined by the biochemical analyzer were 1.0, 6.3, and 6.7 mmol/L (18, 113, and 121 mg/dL), respectively. Our findings suggest that, when using this veterinary PBG, the accuracy of a glucose measurement obtained is higher when using whole blood compared to plasma or serum. Use of whole blood allows for more correct assessment and diagnosis, which are necessary for appropriate therapeutic intervention.


Assuntos
Glicemia/análise , Cães/sangue , Plasma/química , Sistemas Automatizados de Assistência Junto ao Leito/estatística & dados numéricos , Soro/química , Animais , Feminino , Masculino
10.
ACS Sens ; 6(6): 2108-2124, 2021 06 25.
Artigo em Inglês | MEDLINE | ID: mdl-34076428

RESUMO

Readily deployable, low-cost point-of-care medical devices such as lateral flow assays (LFAs), microfluidic paper-based analytical devices (µPADs), and microfluidic thread-based analytical devices (µTADs) are urgently needed in resource-poor settings. Governed by the ASSURED criteria (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverability) set by the World Health Organization, these reliable platforms can screen a myriad of chemical and biological analytes including viruses, bacteria, proteins, electrolytes, and narcotics. The Ebola epidemic in 2014 and the ongoing pandemic of SARS-CoV-2 have exemplified the ever-increasing importance of timely diagnostics to limit the spread of diseases. This review provides a comprehensive survey of LFAs, µPADs, and µTADs that can be deployed in resource-limited settings. The subsequent commercialization of these technologies will benefit the public health, especially in areas where access to healthcare is limited.


Assuntos
COVID-19 , Sistemas Automatizados de Assistência Junto ao Leito , Bioensaio , Humanos , Dispositivos Lab-On-A-Chip , SARS-CoV-2
11.
Anal Chem ; 93(24): 8585-8594, 2021 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-34081452

RESUMO

The global pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has revealed the urgent need for accurate, rapid, and affordable diagnostic tests for epidemic understanding and management by monitoring the population worldwide. Though current diagnostic methods including real-time polymerase chain reaction (RT-PCR) provide sensitive detection of SARS-CoV-2, they require relatively long processing time, equipped laboratory facilities, and highly skilled personnel. Laser-scribed graphene (LSG)-based biosensing platforms have gained enormous attention as miniaturized electrochemical systems, holding an enormous potential as point-of-care (POC) diagnostic tools. We describe here a miniaturized LSG-based electrochemical sensing scheme for coronavirus disease 2019 (COVID-19) diagnosis combined with three-dimensional (3D) gold nanostructures. This electrode was modified with the SARS-CoV-2 spike protein antibody following the proper surface modifications proved by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) characterizations as well as electrochemical techniques. The system was integrated into a handheld POC detection system operated using a custom smartphone application, providing a user-friendly diagnostic platform due to its ease of operation, accessibility, and systematic data management. The analytical features of the electrochemical immunoassay were evaluated using the standard solution of S-protein in the range of 5.0-500 ng/mL with a detection limit of 2.9 ng/mL. A clinical study was carried out on 23 patient blood serum samples with successful COVID-19 diagnosis, compared to the commercial RT-PCR, antibody blood test, and enzyme-linked immunosorbent assay (ELISA) IgG and IgA test results. Our test provides faster results compared to commercial diagnostic tools and offers a promising alternative solution for next-generation POC applications.


Assuntos
Técnicas Biossensoriais , COVID-19 , Grafite , Anticorpos Antivirais , Teste para COVID-19 , Ouro , Humanos , Lasers , Sistemas Automatizados de Assistência Junto ao Leito , SARS-CoV-2 , Sensibilidade e Especificidade , Glicoproteína da Espícula de Coronavírus
12.
IEEE Pulse ; 12(3): 21-23, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1280250

RESUMO

In the wake of the COVID-19 pandemic, the need for rapid and accurate diagnostic testing across populations quickly became evident. In response, the National Institutes of Health (NIH) was determined not only to invest heavily in this area but to change the process by which grant proposals were reviewed and funded in order to spur faster development of viable technologies. The Rapid Acceleration of Diagnostics (RADx) initiative was designed to speed innovation, commercialization, and implementation of potential COVID-19 diagnostic technology. As part of this effort, the RADx Tech initiative focuses on the development, validation, and commercialization of innovative point-of-care, home-based, and clinical lab-based tests that can detect SARS-CoV-2. This effort was enabled through the NIH's National Institute of Biomedical Imaging and Bioengineering (NIBIB) Point-of-Care Technology Research Network (POCTRN).


Assuntos
Engenharia Biomédica/economia , Teste para COVID-19/economia , COVID-19 , National Institutes of Health (U.S.)/economia , Pandemias , Sistemas Automatizados de Assistência Junto ao Leito/economia , SARS-CoV-2 , Engenharia Biomédica/tendências , COVID-19/diagnóstico , COVID-19/economia , COVID-19/epidemiologia , Humanos , Estados Unidos
13.
Biochem Med (Zagreb) ; 31(2): 020601, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: covidwho-1278713

RESUMO

Early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and diagnosis of coronavirus disease 2019 (COVID-19) are priorities during the pandemic. Symptomatic and suspected asymptomatic individuals should be tested for COVID-19 to confirm infection and to be excluded from social interactions. As molecular testing capacity is overloaded during the pandemic, rapid antigen tests, such as lateral flow immunoassays (LFIAs), can be a useful tool as they allow greater test availability and obtain results in a very short time. This short review aims to present the analytical properties of LFIAs in the detection of SARS-CoV-2 in nasopharyngeal swabs. Lateral flow immunoassay is a method that combines thin-layer chromatography and indirect immunochemical sandwich method and allows the detection of a specific SARS-CoV-2 antigen in nasopharyngeal swabs. Swab specimens should be adequately collected and tested as soon as possible. Users should pay attention to quality control and possible interferences. Antigen tests for SARS-CoV-2 show high sensitivity and specificity in cases with high viral loads, and should be used up to five days after the onset of the first symptoms of COVID-19. False positive results may be obtained when screening large populations with a low prevalence of COVID-19 infection, while false negative results may happen due to improper specimen collection or insufficient amount of antigen in the specimen. So as to achieve reliable results, a diagnostic accuracy study of a specific rapid antigen test should be performed.


Assuntos
COVID-19/diagnóstico , Imunoensaio/métodos , Nasofaringe/virologia , SARS-CoV-2/metabolismo , Antígenos Virais/análise , COVID-19/virologia , Reações Falso-Negativas , Humanos , Imunoensaio/normas , Limite de Detecção , Sistemas Automatizados de Assistência Junto ao Leito , Controle de Qualidade , SARS-CoV-2/imunologia , SARS-CoV-2/isolamento & purificação , Sensibilidade e Especificidade , Carga Viral
14.
ACS Sens ; 6(6): 2108-2124, 2021 06 25.
Artigo em Inglês | MEDLINE | ID: covidwho-1253892

RESUMO

Readily deployable, low-cost point-of-care medical devices such as lateral flow assays (LFAs), microfluidic paper-based analytical devices (µPADs), and microfluidic thread-based analytical devices (µTADs) are urgently needed in resource-poor settings. Governed by the ASSURED criteria (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverability) set by the World Health Organization, these reliable platforms can screen a myriad of chemical and biological analytes including viruses, bacteria, proteins, electrolytes, and narcotics. The Ebola epidemic in 2014 and the ongoing pandemic of SARS-CoV-2 have exemplified the ever-increasing importance of timely diagnostics to limit the spread of diseases. This review provides a comprehensive survey of LFAs, µPADs, and µTADs that can be deployed in resource-limited settings. The subsequent commercialization of these technologies will benefit the public health, especially in areas where access to healthcare is limited.


Assuntos
COVID-19 , Sistemas Automatizados de Assistência Junto ao Leito , Bioensaio , Humanos , Dispositivos Lab-On-A-Chip , SARS-CoV-2
15.
Anal Chem ; 93(24): 8585-8594, 2021 06 22.
Artigo em Inglês | MEDLINE | ID: covidwho-1253862

RESUMO

The global pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has revealed the urgent need for accurate, rapid, and affordable diagnostic tests for epidemic understanding and management by monitoring the population worldwide. Though current diagnostic methods including real-time polymerase chain reaction (RT-PCR) provide sensitive detection of SARS-CoV-2, they require relatively long processing time, equipped laboratory facilities, and highly skilled personnel. Laser-scribed graphene (LSG)-based biosensing platforms have gained enormous attention as miniaturized electrochemical systems, holding an enormous potential as point-of-care (POC) diagnostic tools. We describe here a miniaturized LSG-based electrochemical sensing scheme for coronavirus disease 2019 (COVID-19) diagnosis combined with three-dimensional (3D) gold nanostructures. This electrode was modified with the SARS-CoV-2 spike protein antibody following the proper surface modifications proved by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) characterizations as well as electrochemical techniques. The system was integrated into a handheld POC detection system operated using a custom smartphone application, providing a user-friendly diagnostic platform due to its ease of operation, accessibility, and systematic data management. The analytical features of the electrochemical immunoassay were evaluated using the standard solution of S-protein in the range of 5.0-500 ng/mL with a detection limit of 2.9 ng/mL. A clinical study was carried out on 23 patient blood serum samples with successful COVID-19 diagnosis, compared to the commercial RT-PCR, antibody blood test, and enzyme-linked immunosorbent assay (ELISA) IgG and IgA test results. Our test provides faster results compared to commercial diagnostic tools and offers a promising alternative solution for next-generation POC applications.


Assuntos
Técnicas Biossensoriais , COVID-19 , Grafite , Anticorpos Antivirais , Teste para COVID-19 , Ouro , Humanos , Lasers , Sistemas Automatizados de Assistência Junto ao Leito , SARS-CoV-2 , Sensibilidade e Especificidade , Glicoproteína da Espícula de Coronavírus
16.
J Am Dent Assoc ; 152(7): 514-525.e8, 2021 07.
Artigo em Inglês | MEDLINE | ID: covidwho-1212980

RESUMO

BACKGROUND: The dental office potentially possesses all transmission risk factors for severe acute respiratory syndrome coronavirus 2. Anticipating the future widespread use of COVID-19 testing in dental offices, the authors wrote this article as a proactive effort to provide dental health care providers with current and necessary information surrounding the topic. METHODS: The authors consulted all relevant and current guidelines from the Centers for Disease Control and Prevention and the US Food and Drug Administration, as well as online resources and review articles. RESULTS: Routine COVID-19 screening and triage protocols are unable to detect all infected people. With the advancements in diagnostic tools and techniques, COVID-19 testing at home or in the dental office may provide dentists with the ability to evaluate the disease status of their patients. At-home or point-of-care (POC) tests, providing results within minutes of being administered, would allow for appropriate measures and rapid decisions about dental patients' care process. In this review, the authors provide information about available laboratory and POC COVID-19 screening methods and identify and elaborate on the options available for use by dentists as well as the regulatory requirements of test administration. CONCLUSIONS: Dentists need to be familiar with COVID-19 POC testing options. In addition to contributing to public health, such tests may deliver rapid, accurate, and actionable results to clinical and infection control teams to enhance the safe patient flow in dental practices. PRACTICAL IMPLICATIONS: Oral health care must continue to offer safety in this or any future pandemics. Testing for severe acute respiratory syndrome coronavirus 2 at the POC offers a control mechanism contributing to and enhancing the real and perceived safety of care in the dental office setting.


Assuntos
Teste para COVID-19 , COVID-19 , Consultórios Odontológicos , Humanos , Laboratórios , Sistemas Automatizados de Assistência Junto ao Leito , SARS-CoV-2
18.
J Am Dent Assoc ; 152(7): 514-525.e8, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34176567

RESUMO

BACKGROUND: The dental office potentially possesses all transmission risk factors for severe acute respiratory syndrome coronavirus 2. Anticipating the future widespread use of COVID-19 testing in dental offices, the authors wrote this article as a proactive effort to provide dental health care providers with current and necessary information surrounding the topic. METHODS: The authors consulted all relevant and current guidelines from the Centers for Disease Control and Prevention and the US Food and Drug Administration, as well as online resources and review articles. RESULTS: Routine COVID-19 screening and triage protocols are unable to detect all infected people. With the advancements in diagnostic tools and techniques, COVID-19 testing at home or in the dental office may provide dentists with the ability to evaluate the disease status of their patients. At-home or point-of-care (POC) tests, providing results within minutes of being administered, would allow for appropriate measures and rapid decisions about dental patients' care process. In this review, the authors provide information about available laboratory and POC COVID-19 screening methods and identify and elaborate on the options available for use by dentists as well as the regulatory requirements of test administration. CONCLUSIONS: Dentists need to be familiar with COVID-19 POC testing options. In addition to contributing to public health, such tests may deliver rapid, accurate, and actionable results to clinical and infection control teams to enhance the safe patient flow in dental practices. PRACTICAL IMPLICATIONS: Oral health care must continue to offer safety in this or any future pandemics. Testing for severe acute respiratory syndrome coronavirus 2 at the POC offers a control mechanism contributing to and enhancing the real and perceived safety of care in the dental office setting.


Assuntos
Teste para COVID-19 , COVID-19 , Consultórios Odontológicos , Humanos , Laboratórios , Sistemas Automatizados de Assistência Junto ao Leito , SARS-CoV-2
19.
Anal Chim Acta ; 1170: 338630, 2021 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-34090590

RESUMO

Smartphone-assisted point-of-care (POC) bioassay has brought a giant leap in personal healthcare system and environmental monitoring advancements. In this study, we developed a rapid and reliable colorimetric urea biosensor assisted by a smartphone. We employed hydrolysis of urea into NH3 by urease, which activates the reduction power of tannic acid, to generate silver nanoparticles for a dramatic colorimetric response. The proposed urea biosensor was validated in a solution to provide high selectivity against various interferents in human urine. It had high sensitivity, with a limit of detection as low as 0.0036 mM, and a high reliability of 99% ± 2.9% via the standard addition method. The urea biosensor was successfully implanted on a paper to facilitate smartphone-assisted POC readout with a limit of detection of 0.58 mM and wide detection range of 500 mM, whereby direct diagnosis of human urine without dilution was realized. Our smartphone-assisted POC colorimetric urea biosensor will pave the way for daily monitoring systems of renal and hepatic dysfunction diseases.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Colorimetria , Humanos , Concentração de Íons de Hidrogênio , Sistemas Automatizados de Assistência Junto ao Leito , Reprodutibilidade dos Testes , Prata , Smartphone , Ureia
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