Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 694
Filtrar
1.
PLoS One ; 17(5): e0267409, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35500007

RESUMO

The objective of this study was to investigate the nutritional quality of bovine colostrum and whey mixtures. Five whey with bovine colostrum formulations were prepared (90:10; 80:20; 70:30; 60:40 and 50:50 whey:colostrum v:v) to be subjected to low-temperature pasteurization (63°C to 65°C for 30 minutes) and freeze-drying. The samples underwent chemical composition characterization, fatty acid profile analysis, determination of contamination by Enterobacteriaceae, pH, and Dornic acidity measurements before and after vat pasteurization. The amount of protein, fat, total solids, defatted dry extract, Brix and density increased as the bovine colostrum concentration increased. The level of saturated fatty acids and the thrombogenicity and atherogenicity indices reduced, while unsaturated fatty acids increased as the level of added bovine colostrum increased. The low-temperature pasteurization of the formulations was possible and effective, eliminating contamination by Enterobacteriaceae in the samples. Mixing bovine colostrum and whey reduced the colostrum viscosity, allowing a successful pasteurization procedure. Due to colostrum composition, the formulations yielded a higher nutritional value when compared to whey alone. The parameters applied in the formulation of mixtures of bovine colostrum and whey resulted in valuable ingredients for preparing novel dairy products.


Assuntos
Colostro , Soro do Leite , Animais , Bovinos , Colostro/química , Ácidos Graxos/metabolismo , Feminino , Gravidez , Viscosidade , Soro do Leite/química , Proteínas do Soro do Leite/metabolismo
2.
Nutrients ; 14(8)2022 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-35458155

RESUMO

Fish is considered an important part of a healthy diet, in part due to the content of long chain omega-3 fatty acids. However, both lean and fatty fish have beneficial health effects, suggesting that micronutrients and proteins may play a role. In a randomised, controlled, cross-over trial, five healthy male participants consumed 5.2 g of protein from either salmon fishmeal or whey. Blood samples were taken before and 30 and 60 min after intake. The concentration of glucose, lipids, hormones and metabolites, including 28 different amino acids and derivatives, were measured in serum or plasma. Cultured HepG2 cells were incubated with or without serum from the participants, and transcriptomic profiling was performed using RNA sequencing. The ingestion of both salmon fishmeal and whey reduced the glucose and triglyceride levels in serum. Protein intake, independent of the source, increased the concentration of 22 amino acids and derivatives in serum. Fishmeal increased the concentration of arginine, methionine, serine, glycine, cystathionine and 2-aminobutyric acid more than whey did. Incubation with postprandial serum resulted in large transcriptomic alterations in serum-fasted HepG2 cells, with the differential expression of >4500 protein coding genes. However, when comparing cells cultivated in fasting serum to postprandial serum after the ingestion of fishmeal and whey, we did not detect any differentially regulated genes, neither with respect to the protein source nor with respect to the time after the meal. The comparable nutrigenomic effects of fishmeal and whey do not change the relevance of fish by-products as an alternative food source.


Assuntos
Salmão , Soro do Leite , Aminoácidos , Animais , Glicemia/metabolismo , Estudos Cross-Over , Expressão Gênica , Glucose , Humanos , Fígado/metabolismo , Masculino , Período Pós-Prandial , Salmão/metabolismo , Soro do Leite/metabolismo , Proteínas do Soro do Leite
3.
Viruses ; 14(4)2022 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-35458540

RESUMO

Fifteen samples of whey protein concentrate (WPC) were tested against 37 commercial Streptococcus thermophilus strains to detect infective bacteriophages. Seventy-three diverse phages were isolated from 12 samples, characterized by using DNA restriction patterns and host range analyses. Sixty-two of them were classified as cos, two as pac, and nine as 5093, according to PCR multiplex assays. Phage concentration was greater than 104 PFU/g for 25.3% of isolated phages. Seven phages showed an unusual wide host range, being able to infect a high number of the tested strains. Regarding thermal resistance, pac phages were the most sensitive, followed by cos phages, those classified as 5093 being the most resistant. Treatments at 85 °C for 5 min in TMG buffer were necessary to completely inactivate all phages. Results demonstrated that the use, without control, of these whey derivatives as additives in dairy fermentations could be a threat because of the potential phage infection of starter strains. In this sense, these phages constitute a pool of new isolates used to improve the phage resistance of starter cultures applied today in the fermentative industry.


Assuntos
Bacteriófagos , Fagos de Streptococcus , Bacteriófagos/genética , Indústria de Laticínios , Streptococcus thermophilus/metabolismo , Soro do Leite , Proteínas do Soro do Leite/metabolismo
4.
Nutrients ; 14(7)2022 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-35405988

RESUMO

Whey protein and its hydrolysate are ubiquitously consumed as nutritional supplements. This study aimed to evaluate the potential effect of whey protein hydrolysate (WPH) on the infant gut microbiome, which is more variable than that of adults. Colonic fermentation was simulated through a static digestion model and fecal culture fermentation, using feces from normal infants aged from 1-3 years old. During in vitro gut fermentation, measurements of short-chain fatty acids (SCFA) concentrations and 16S rRNA amplicon sequencing were performed. Additionally, the growth curves of cultivated probiotics were analyzed to evaluate the prebiotic potential of WPH. Besides the decline of pH in fermentation, the addition of WPH induced a significant increase in the SCFA production and also the relative abundance of Proteobacteria, Bacteroides, and Streptococcus (p < 0.05). The lower ratio of Firmicutes/Bacteroidetes in WPH-supplemented samples indicated the positive modulation of WPH on the gut microbiota, which could benefit the energy balance and metabolism of infants. The stimulation effect of WPH on the probiotics (particularly Lactobacillus acidophilus NCFM) during cultivation implied the prebiotic potential as well. Our findings shed light on WPH as a valuable dietary supplement with not only enriched resources of essential amino acids but also the potential to restore the infant gut microbiome.


Assuntos
Microbioma Gastrointestinal , Hidrolisados de Proteína , Soro do Leite , Bactérias , Pré-Escolar , Ácidos Graxos Voláteis/metabolismo , Fezes/microbiologia , Fermentação , Microbioma Gastrointestinal/fisiologia , Humanos , Lactente , Prebióticos/análise , Hidrolisados de Proteína/metabolismo , RNA Ribossômico 16S/metabolismo , Soro do Leite/metabolismo
5.
Food Funct ; 13(7): 3957-3967, 2022 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-35293905

RESUMO

A phenylalanine (Phe)-restricted diet is indispensable to control the blood Phe for individuals with phenylketonuria (PKU), who are also confronted with progressive bone impairment. Thus, the development of a low-Phe protein substitute that could positively regulate bone metabolism is desired for their bone health. Our previous study reported the preparation of a low-Phe containing whey hydrolysate (LPH) from a selected whey protein hydrolysate (TAH). However, the effect of LPH on the bone status is unknown. In this study, we used an ovariectomized (OVX) mice model to evaluate the anti-osteoporotic potential of oral administration of whey protein concentrate (WPC, protein control), TAH, and LPH on bone physiology and bone metabolism. The results showed that after 12 weeks of treatment, the decreased bone mineral density, the deteriorated trabecular microarchitecture, and the reduced ultimate load due to ovariectomy were significantly attenuated by two whey protein hydrolysates (TAH and LPH); meanwhile, the body weight, uterine weight, bone composition, and the femoral elastic load of OVX mice had not been significantly affected by whey samples. In addition, LPH and TAH dual-regulated bone remodeling in OVX mice through triggering osteogenesis (promoted the expression of runt-related protein 2 (Runx2) and osteoformation markers) and inhibiting osteoresorption as well as inflammation. The modulated mitogen-activated protein kinase signaling and the inhibited nuclear factor κB signaling by LPH and TAH might relate to the dual-regulatory activities on bone. Overall, in the OVX mice model, LPH exerted higher osteoprotective potential than TAH of the same dose by activating the bone formation markers and inhibiting the inflammatory status. The current study demonstrated for the first time the potential use of a low-Phe whey hydrolysate, a protein substitute for PKU individuals, in the prevention of osteoporosis.


Assuntos
Osteoporose , Hidrolisados de Proteína , Animais , Densidade Óssea , Feminino , Humanos , Camundongos , Osteogênese , Osteoporose/tratamento farmacológico , Ovariectomia , Fenilalanina/farmacologia , Hidrolisados de Proteína/farmacologia , Hidrolisados de Proteína/uso terapêutico , Soro do Leite
6.
J Nutr Health Aging ; 26(2): 127-132, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35166303

RESUMO

CONTEXT: Epidemiological studies have shown that consumption of dairy products reduces the risk of dementia and cognitive decline in older individuals. Tryptophan-tyrosine-related ß-lactopeptides and their representative ß-lactolin of glycine-threonine-tryptophan-tyrosine tetra-peptide have been identified as agents in dairy products, which improve cognitive function as well as memory function via the activation of the dopaminergic system in a mouse model of amnesia. Previous clinical trials have shown that supplementation with ß-lactolin improves memory retrieval in healthy older adults. Specifically, ß-lactolin improved the scores in some neuropsychological tests. However, the effects of ß-lactolin on memory function have not been clarified. OBJECTIVES: The aim of this study was to evaluate the effect of ß-lactolin on memory function using statistical methods. DATA SOURCES: We searched the Web of Science, Cochrane Library, and JDream III until November 2021 to identify relevant randomized controlled trials for integrated analysis. DATA SYNTHESIS: Three randomized controlled trials evaluating the effect of ß-lactolin on memory in healthy adults were selected for the integrated analysis. The results showed that the score of cued recall among the neuropsychological tests in the ß-lactolin group was significantly higher than that in the placebo group (g=0.33; 95% CI: 0.10, 0.55). In addition, the total memory score was higher but this difference was not significant (g=0.17; 95% CI: -0.09, 0.43). CONCLUSIONS: Taken together, these results suggest that supplementation with ß-lactolin improves cued recall in healthy older adults.


Assuntos
Oligopeptídeos , Soro do Leite , Animais , Cognição , Camundongos , Oligopeptídeos/farmacologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Proteínas do Soro do Leite/farmacologia
7.
J Environ Manage ; 308: 114581, 2022 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-35124319

RESUMO

Cheese whey is an industrial waste generated from the cheese processing unit of the dairy industry and requires treatment before its disposal. The present study investigated the possibilities of improving the digestibility from anaerobic digestion of lipid rich dairy by-product, cheese whey using septage as the co-substrate with different inoculum. Biochemical methane potential assays were conducted under mesophilic temperature conditions and results were validated using Modified Gompertz Model. Two sets of BMP tests were done; to assess the individual and combined digestion abilities of septage in anaerobic co-digestion of whey and to assess the ability of 3 inoculum sources (cattle manure, sewage sludge, and acclimatized anaerobic sludge) in the co-digestion process. The results indicated that septage is an excellent co-substrate that has better adaptability with cheese whey and the optimum mix ratio was found as 40:60 (SCW: SSP). BMP tests were also conducted with inoculum at S/I ratio of 1 and statistical analysis was performed to study the synergistic effect of both co-digestion and inoculum. The tests revealed that the cattle manure resulted in the highest biogas production (342.22mL/gVS) at 60% whey fraction. Modified Gompertz model fitted the experimental data well and identified an increase in lag phase times when whey fraction is increased. Comparatively higher lag phase times ranging from 1.98 to 4.35 days were obtained for sewage sludge inoculated samples. The maximum methane production (Pmax) was obtained at 60% whey fraction (369.63 ± 4.05mL/gVS) at a very short lag time of 0.76 ± 0.17days for cattle manure inoculated mixture.


Assuntos
Biocombustíveis , Queijo , Anaerobiose , Animais , Biocombustíveis/análise , Reatores Biológicos , Bovinos , Queijo/análise , Digestão , Esterco/análise , Metano/análise , Esgotos , Soro do Leite/química
8.
Nutrients ; 14(4)2022 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-35215448

RESUMO

Sarcopenia is prevalent as the aging population grows. Therefore, the need for supplements for the elderly is increasing. This study aimed to investigate the efficacy and mechanism of a Panax ginseng berry extract (GBE) and soluble whey protein hydrolysate (WPH) mixture on a sarcopenia-related muscular deterioration in aged mice. Ten-month-old male C57BL/6J mice were administered three different doses of the GBE + WPH mixture for 8 weeks; 700 mg/kg, 900 mg/kg, and 1100 mg/kg. Grip strength, serum inflammatory cytokines level, and mass of muscle tissues were estimated. The deteriorating function of aging muscle was investigated via protein or gene expression. Grip strength and mass of three muscle tissues were increased significantly in a dose-dependent manner, and increased anti-inflammatory cytokine alleviated systemic inflammatory state. The mixture resolved the imbalance of muscle protein turnover through activation of the PI3K/Akt pathway and increased gene expression of the muscle regeneration-related factors, while decreasing myostatin, which interferes with muscle protein synthesis and regeneration. Furthermore, we confirmed that increased mitochondria number in muscle with the improvement of mitochondrial biogenesis. These physiological changes were similar to the effects of exercise.


Assuntos
Panax , Sarcopenia , Animais , Frutas/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Músculo Esquelético/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Extratos Vegetais/metabolismo , Extratos Vegetais/farmacologia , Hidrolisados de Proteína/metabolismo , Hidrolisados de Proteína/farmacologia , Hidrolisados de Proteína/uso terapêutico , Soro do Leite/metabolismo , Proteínas do Soro do Leite/metabolismo , Proteínas do Soro do Leite/farmacologia
9.
Anal Methods ; 14(8): 813-819, 2022 02 24.
Artigo em Inglês | MEDLINE | ID: mdl-35138313

RESUMO

Herein, we present a facile and sensitive fluorescence resonance energy transfer (FRET) aptasensor for the detection of pathogenic bacteria, where antibiotic-functionalized cerium oxide nanoparticles were served as an energy donor and aptamer-modified gold nanoparticles (aptamer-AuNPs) were employed as an energy acceptor. To illustrate the feasibility of this strategy, Escherichia coli (E. coli) was examined. The strategy for the detection of E. coli bacteria as a target molecule is described using the FRET pair of azithromycin-functionalized CeO2 nanoparticles (Azm-CeO2NPs) and aptamer-AuNPs. The spectral overlap between these two nanoparticles and Azm and the aptamer binding on the surface of E. coli specifically provides the condition, which leads to the occurrence of the FRET phenomenon. In this way, a good linear correlation between the fluorescence intensity of Azm-CeO2NPs and E. coli concentration was obtained in the range of 10-1.5 × 105 cfu mL-1. The detection limit of the proposed method at a signal to noise ratio of 3 (3σ) was estimated to be 1.04 cfu mL-1. Further, the proposed method was applied to detect E. coli in real samples within 30 min, which indicates the applicability of the proposed method. This method could be used for other pathogenic bacterium recognition or synchronous detection by employing molecules that are particular to the desired bacteria.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Nanopartículas Metálicas , Animais , Aptâmeros de Nucleotídeos/análise , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Cério , Escherichia coli , Transferência Ressonante de Energia de Fluorescência/métodos , Ouro/química , Nanopartículas Metálicas/química , Leite/química , Soro do Leite
10.
PLoS One ; 17(1): e0262940, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35085345

RESUMO

Inoculation dose is a key operational parameter for the solid-state anaerobic digestion (SS-AD) of lignocellulosic biomass, maximum methane recovery, and stable digester performance. The novelty of this study was the co-digestion of unamended full-strength grape marc and cheese whey for peak methane extraction at variable inoculation levels. An acclimatised digestate from a preceding anaerobic treatment was used as a downstream inoculum. The impact of inoculum size (wet weight) was evaluated at 0/10, 5/5, 7/3 and 9/1 substrate-to-inoculum (S/I) ratios, corresponding to an initial concentration of 20-30% total solids (TS) in digesters over 58 days at 45°C. The optimal 7/3 S/I produced the highest cumulative methane yield, 6.45 L CH4 kg-1 VS, coinciding with the lowest initial salinity at 11%; the highest volumetric methane productivity rate of 0.289±0.044 L CH4 LWork-1 d-1; the highest average COD/N ratio of 9.88; the highest final pH of 9.13, and a maximum 15.07% elemental carbon removal; for a lag time of 9.4 days. This study identified an optimal inoculation dose and opens up an avenue for the direct co-digestion of grape marc and cheese whey without requirements for substrate pretreatment, thus improving the overall bioenergy profile of the winery and dairy joint resource recovery operations.


Assuntos
Biocombustíveis , Reatores Biológicos , Queijo , Vitis , Soro do Leite , Anaerobiose
11.
J Dairy Sci ; 105(3): 1900-1912, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35086699

RESUMO

The aim of the present study was to investigate the antiinflammatory and antibiofilm effects of whey fermented by Enterococcus faecalis M157 (M157-W) against oral pathogenic bacteria. The M157-W significantly inhibited IL-1ß, IL-6, and nitric oxide induced by the lipopolysaccharide of Porphyromonas gingivalis in RAW 264.7 cells. The M157-W also inhibited the production of IL-1ß and IL-8 in human periodontal ligament cells. Treatment with M157-W suppressed the phosphorylation of mitogen-activated protein kinases as well as the activation of nuclear factor-κB in RAW 264.7 cells stimulated by P. gingivalis lipopolysaccharide. Furthermore, M157-W dose-dependently inhibited Streptococcus mutans biofilm, whereas unfermented whey did not inhibit the biofilm. Treatment with M157-W significantly suppressed gtfB, gtfC, and gtfD gene expression in S. mutans compared with the control (0 µg/mL), indicating that M157-W inhibits S. mutans biofilm formation by reducing the synthesis of extracellular polymeric substances. Collectively, these results suggest that M157-W has antiinflammatory and antibiofilm activities against oral pathogenic bacteria.


Assuntos
Enterococcus faecalis , Soro do Leite , Animais , Biofilmes , Lipopolissacarídeos/farmacologia , Streptococcus mutans/genética
12.
Diabetes Metab Syndr ; 16(1): 102372, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34998259

RESUMO

BACKGROUND AND AIMS: Due to inconsistent data about WP supplementation on inflammatory markers, present systematic review and meta-analysis was done to summarize its effect on TNF-α and IL-6. METHODS: Our search was done in Pubmed, Scopus, Embase, and Cochrane up to June 2021. Weighted mean difference (WMD) and 95% confidence intervals (CI) was used to indicate the effect sizes. Conceivable sources of heterogeneity were detected by subgroup analysis. RESULTS: Overall, 11 eligible RCTs were included. The pooled results showed that WP supplementation had no significant effect on TNF-α and IL-6 status compare to those receiving carbohydrate and other types of proteins as placebo. Results from subgroup analysis based on health status, study duration, WP dosage and sex, expressed no favorable effect of WP on TNF-α and IL-6 levels. CONCLUSION: It can be concluded that whey supplementation had no favorable effects on inflammatory biomarkers including TNF- α and IL-6.


Assuntos
Interleucina-6 , Fator de Necrose Tumoral alfa , Biomarcadores/metabolismo , Suplementos Nutricionais , Humanos , Inflamação/metabolismo , Ensaios Clínicos Controlados Aleatórios como Assunto , Soro do Leite/metabolismo , Proteínas do Soro do Leite
13.
Food Chem ; 380: 132193, 2022 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-35093650

RESUMO

New technologies for food processing have been used to enhance the beneficial effects of foods. This study aimed to evaluate the effects of a prebiotic soursop whey beverage processed by high-intensity ultrasound (HIUS) on healthy rats. Whey beverages were processed by HIUS (20KHz, 520 W of nominal power, <53 °C, 20.3 W of acoustic power, energy density of 2.9 kJ.cm-3 and 9.5 min to process 100 mL) and high-temperature short-time (HTST, 75 °C for 15 s) before being supplemented to Wistar rats by gavage for 15 days. Antioxidant, antidiabetic, anti-hypertensive, and anticancer activities, lipid peroxidation, bioactive peptides, and microstructure of the beverages were analyzed. In addition, the body mass, food, and water intake, systolic blood pressure, biochemical and oxidative stress parameters were measured. The sonicated beverage induced satiety, decreased glutathione peroxidase activity, total triglycerides, very-low-density lipoprotein cholesterol, and alanine aminotransferase. These findings suggest that ultrasound technology can provide in vivo health and functional benefits.


Assuntos
Annona , Soro do Leite , Animais , Antioxidantes/análise , Bebidas/análise , Prebióticos , Ratos , Ratos Wistar , Soro do Leite/química
14.
Chemosphere ; 293: 133567, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35026199

RESUMO

In order to set up a cost-efficient biorefinery in a Tunisian dairy industry, the production unit effluents are recovered. The main objective is to develop an optimum method for the production of bioethanol from whey. An energy analysis as well as environmental and economic analyses are performed for a bioethanol production plant. Four production scenarios are examined in order to determine the most provident as well as the less polluting ones. The process and cost models were developed using SuperPro Designer software which a simulation program that is able to estimate both process and economic parameters. This software uses energy and mass balances. The model can be used to assess the efficiency, the resources consumption, the profitability and the environmental impact of each scenario. The results demonstrate that the third scenario, in which a reverse osmosis procedure is added to concentrate the whey, a continuous stoichiometric reaction procedure is integrated to model the biotransformation in the fermenter and where streams are added in order to recycle the biomass, produces the highest amount of bioethanol with 1.65 MT/year but the second one (where no streams were added) is the most profitable one with revenues as high as 570 000 $/year. The corresponding cost of ethanol production is 0.271 US $ ethanol per liter. The net present value (NPV) and the return on investment (ROI) of each scenario are positive. Such result indicates that all these investments could be undertaken in order to find an eco-friendly issue for the dairy industry effluents. Cheese whey could serve as an alternative raw material for producing ethanol.


Assuntos
Queijo , Soro do Leite , Indústria de Laticínios , Etanol/metabolismo , Soro do Leite/metabolismo , Proteínas do Soro do Leite/metabolismo
15.
J Environ Manage ; 304: 114253, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-35021584

RESUMO

BioH2 production from cheese whey (CW) was evaluated in two acidogenic reactors, UASB and structured fixed-bed (FB), without pH adjustment, under mesophilic conditions, and OLR of 25-90 g COD/L.d. Stage 1 was conducted as a control experiment using sucrose. BioH2 production occurred under pH < 3.0 with maximum yields of 5.8 and 3.0 mol H2/mol sucroseconsumed for UASB and FB reactors, respectively. In Stage 2, CW was the only substrate and a negligible bioH2 production was observed. Nevertheless, a maximum lactic acid concentration of 9.6 g/L was obtained, indicating that pH adjustment can be non-essential for lactic acid production from CW. In Stage 3, a strategy to enrich hydrogenogenic biomass was conducted by initially feeding the reactors with sucrose and gradually replacing it by CW. This strategy brought better bioH2 results compared to Stage 2, but it could not bear over the long-term, as non-hydrogen producing bacteria became predominant.


Assuntos
Queijo , Soro do Leite , Anaerobiose , Reatores Biológicos , Fermentação , Hidrogênio , Metano
16.
J Microbiol Biotechnol ; 32(1): 64-71, 2022 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-34675139

RESUMO

The discarding of wastes into the environment is a significant problem for many communities. Still, food waste can be used for lactic acid bacteria (LAB) growth. Here, we evaluated three growth media equivalent to de Mann Rogosa Sharpe (MRS), using apple bagasse, yeast waste, fish flour, forage oats, and cheese whey. Cell-free supernatants of eight LAB strains were tested for antimicrobial activity against nine indicator microorganisms. The supernatants were also evaluated for protein content, reducing sugars, pH, and lactic acid concentration. Cell-free supernatants from fish flour broth (FFB) LAB growth were the most effective. The strain Leuconostoc mesenteroides PIM5 presented the best activity in all media. L. mesenteroides CAL14 completely inhibited L. monocytogenes and strongly inhibited Bacillus cereus (91.1%). The strain L. mesenteroides PIM5 consumed more proteins (77.42%) and reducing sugars (56.08%) in FFB than in MRS broth (51.78% and 30.58%, respectively). Culture media formulated with agroindustrial wastes positively improved the antimicrobial activity of selected LAB, probably due to the production of antimicrobial peptides or bacteriocins.


Assuntos
Anti-Infecciosos/farmacologia , Meios de Cultura/química , Lactobacillales/fisiologia , Águas Residuárias/química , Animais , Bacillus cereus , Queijo , Fermentação , Microbiologia de Alimentos , Lactobacillus , Leuconostoc mesenteroides/efeitos dos fármacos , Listeria monocytogenes/efeitos dos fármacos , Soro do Leite
17.
J Dairy Sci ; 105(1): 83-96, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34635352

RESUMO

This research aimed to advance the understanding of acceptable sensory qualities of potable whey-based spirit from nonsupplemented, mid-supplemented, and high-supplemented whey samples by analyzing major volatile compounds during different stages of distillation (head, heart, and tail). The results demonstrated that commercial Saccharomyces cerevisiae strain in lactase-hydrolyzed whey showed rapid and complete sugar hydrolysis and efficient ethanol production in 24, 30, and 36 h on average, producing up to 29.5, 42.1, and 56.4 g/L of ethanol, respectively. The variations in titratable acidity, specific gravity, pH value, residual protein, sugar content, and alcohol yield were investigated during the fermentation. The total amount of volatile compound concentrations significantly decreased from the head (2,087-2,549 mg/L) to the tail whey spirits (890-1,407 mg/L). In the whey spirit, 2-methyl-1-butanol, 3-methyl-1-butanol, 2-methyl-1-propanol, 1-propanol, acetaldehyde, and ethyl acetate were the most prevalent dominant compounds, accounting for the largest proportion of total volatile compounds. The volatile compounds detected were far below the acceptable legal limit. The results suggest that high sensory qualities of potable whey-based spirits can be produced by fermentation of lactose-supplemented whey with S. cerevisiae cells.


Assuntos
Lactose , Soro do Leite , Animais , Destilação , Fermentação , Saccharomyces cerevisiae , Proteínas do Soro do Leite
18.
Artigo em Inglês | MEDLINE | ID: mdl-34506720

RESUMO

The aim of this study was to investigate the transfer of cephalexin, penicillin-G, and ampicillin & cloxacillin from cow's milk to cheese and whey. For this purpose, raw milk was artificially contaminated to different antibiotic levels and then heat-treated to prepare fresh cheese from it. Antibiotic levels of the milk, whey and cheese were measured with LC-MS/MS. The extent of heat degradation was not sufficient to remove the antibiotic residues from milk. Antibiotic concentrations in whey and fresh cheese were in good accordance with the concentration of the same compound in milk suggesting that contamination of the milk will result in contamination of the product. The investigated antibiotics were transferred less into the cheese curd (1.6-12.5% of the original amount), than into the whey (33.2-74.1%). For penicillin-G even 100% (complete removal) was experienced.


Assuntos
Antibacterianos/análise , Queijo/análise , Contaminação de Alimentos/análise , Leite/química , Soro do Leite/química , beta-Lactamas/análise , Ampicilina/química , Animais , Bovinos , Cefalexina/química , Cromatografia Líquida de Alta Pressão , Cloxacilina/química , Feminino , Humanos , Penicilinas/química , Espectrometria de Massas em Tandem
19.
J Environ Manage ; 303: 114138, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34838385

RESUMO

Vinasse and whey are wastewaters that are produced in large quantities in the sugar-to-ethanol and dairy industries, respectively. They pose a considerable threat to the environment due to the high concentration of nutrients and COD. In this study, the potential of producing protein-rich fungal biomass and biomethane from vinasse and whey through a two-stage biorefinery was examined. In the first stage, an edible and safe for human filamentous fungus, Neurospora intermedia, was cultivated on these wastewaters. To maximize the fungal biomass yield, the cultivation parameters, i.e., pH, vinasse to whey ratio, incubation time, and nutrients supplementation, were optimized. The highest yield of 12.0 g biomass per L of wastewaters was obtained by cultivation at pH 6.5 and vinasse to whey ratio of 25:75 (v/v) for 96 h with nitrogen source supplementation. The N. intermedia biomass contained about 45% protein and noticeable essential amino acid contents, comparable to commercial sources of protein for aquatic feed such as soybean meal and fishmeal. In the second stage, the effluent of fungal cultivation was anaerobically digested to produce 425 mL/g VS biomethane. Overall, 1 m3 of wastewater yielded 5.4 kg crude protein and 10.3 m3 methane, accompanied by 93.3% COD removal.


Assuntos
Biocombustíveis , Soro do Leite , Biomassa , Reatores Biológicos , Humanos , Metano
20.
J Environ Manage ; 304: 114194, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34864414

RESUMO

Composting residues from wine and dairy chains would contribute to increase the environmental sustainability of the production. The aim of this study was to evaluate the effects of deproteinized whey combined with bioactivators on the composting process. Bacillus velezensis and Kocuria rhizophila, bacteria with cellulolytic activity, were isolated from raw materials and inoculated in the organic mass to be composted. Piles moistened with deproteinized whey showed the highest reduction of total and dissolved organic carbon due to the stimulation of bacterial activity by nitrogen compounds held within deproteinized whey. Such findings were also confirmed by the speed up of the microbial carbon mineralization. Bioactivators and deproteinized whey speeded up the composting process and returned compost characterized by high stability and quality. The addition of available N is crucial to improve the composting process and can act even better if combined with cellulolytic bacteria.


Assuntos
Compostagem , Vinho , Bactérias , Carbono , Nitrogênio , Soro do Leite
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...