Diterpenes of Pinus pinaster aiton with anti-inflammatory, analgesic, and antibacterial activities.

ETHNO-PHARMACOLOGICAL RELEVANCE: The P. pinaster species, known as 'Pino nigral or rodeno', is used in the treatment of colds, asthma, flu, and tuberculosis. AIM OF THE STUDY: This study determined the anti-inflammatory, analgesic, and antibacterial activities of the P. pinaster resin, identifying the compounds with higher biological activity. MATERIALS AND METHODS: A bio-guided isolation of the compounds of P. pinaster was carried out by selecting the most active extracts with anti-inflammatory and analgesic effects in the HBEC3-KT, MRC-5, and THP-1 cell lines. The antibacterial activity was determined against the S. aureus, S. pneumoniae, K. pneumoniae and P. aeruginosa strains. RESULTS: The following compounds were identified by NMR: dehydroabietic acid (1), ( + )-cis-abienol (2), pimaric acid (3), isopimaric acid (4), 7α-hydroxy-dehydroabietic acid (5), 7-oxo-dehydroabietic acid (6), 15-hydroxy-abietic acid (7), 7-oxo-15-hydroxy-dehydroabietic acid (8), 13-oxo-8 (14)-podocarpen-18-oic acid (9), and pinyunin A (10). Regarding their anti-inflammatory activity, all compounds inhibited NF-κB. Compound 9 was the most active (IC50 = 3.90-12.06 µM). Concerning the analgesic activity, all the compounds inhibited NK-1, yet compound 9 was the most active (IC50 = 0.28-0.33 µM). Finally, compounds 6 (MIC = 12.80-25.55 µM) and 9 (MIC = 9.80-24.31 µM) were the most promising antibacterial compounds in all strains. CONCLUSION: This study managed to identify, for the first time, six diterpenes from the resin of P. pinaster, with anti-inflammatory, analgesic, and antibacterial activity. Among the identified compounds, compound 9 was the most active, being considered a promising candidate as an antagonist of the tachykinin NK-1 receptor and as an analgesic agent against inflammation and neuropathic pain. It also had an antibacterial effect against Gram negative bacteria.

Different crosslinking as a strategy to improve films produced from external mesocarp of pequi (Caryocar brasiliense).

The pequi (Caryocar brasiliense) external mesocarp is rich in phenolic compounds and pectin and demonstrates the potential to produce active and biodegradable films. Thus, the present study aimed to produce films with pequi mesocarp as a polymer matrix and evaluate the influence of crosslinking agents (calcium chloride and citric acid) on the film's properties. The films obtained from pequi mesocarp (MF), showed in general, complete biodegradation in 33 days, good antioxidant capacity, and inhibition against S. aureus (24.7 mm) and E. coli (23.0 mm). The crosslinking agents reduced solubility by up to 35% and increased the elongation of the films by up to 3.5-fold. Calcium chloride promoted a higher reduction in solubility, and both agents increase the antioxidant and antimicrobial activities, compared to MF. Citric acid proved to be the best agent to modify the properties of pequi mesocarp films. In addition to the crosslinking action, it presented plasticizing effect.

Nano-biosensor based on manganese dioxide nanosheets and carbon dots for dual-mode determination of Staphylococcus aureus.

A ratiometric fluorescence and colorimetry dual-mode nano-biosensor has been established for Staphylococcus aureus (S. aureus) determination. The prepared approaches of Manganese dioxide nanosheets (MnO2 NSs) and carbon dots (BCDs) were facile, efficient and labor-saving and MnO2 NSs-mediated fluorescence quenching and oxidation could amplify detection signals. The dual-mode determination had a broad linear range of 37 âˆ¼ 3.7 × 106 CFU/mL and low detection limits of 9 CFU/mL (ratiometric fluorescence) and 22 CFU/mL (colorimetry). Meanwhile, the method was applied in real samples with recovery ranging of 90 âˆ¼ 102% and RSD < 4.44%, which was an insignificant difference with standard plate counting. The new dual-mode approach of S. aureus possesses the advantages of superior sensitivity, precision, accuracy and specificity. Moreover, the dual-mode nano-biosensor can be adopted in other foodborne pathogens determination by changing corresponding aptamers and provide an enlightenment in monitoring food safety.

Biopolymer films incorporated with chlorogenic acid nanoparticles for active food packaging application.

Food packaging is innovating towards more environmental-friendly polymers and broader applications of bioactive compounds. In this study, active packaging materials were successfully prepared by incorporating chlorogenic acid (CGA) nanoparticles into pullulan/gelatin polymer matrixes. The rhamnolipid (RL) and/or CGA were combined with chitosan (CS) to synthesize active nanoparticles by the ionic crosslinking method. The film containing CS/RL/CGA nanoparticles (F/CRC) exhibited both ultrahigh visible light (400-760 nm) transmittance (approximately 90%) and UVA (320-400 nm)-blocking efficiency (89.06%). Its fluorescent properties can be used for anti-counterfeiting. Significantly, the bacterial inhibition rates of F/CRC against E. coli and S. aureus were 92.14% and 98.72%. F/CRC also showed good antioxidant capability and biosafety. Finally, the packaging test further indicated that F/CRC could delay the browning of bananas and the bacteria growth of chicken samples. This work presents a green and feasible route to produce functional materials with UV-shielding properties for packaging applications.

Development of pH-responsive intelligent and active films based on pectin incorporating Schiff base (Phenylalanine/syringaldehyde) for monitoring and preservation of fruits.

This study aimed to develop pectin-based films by incorporating Schiff base compounds (SPS) synthesized by phenylalanine and syringaldehyde. The SEM images showed good compatibility between SPS and pectin matrix. The interaction of SPS and pectin matrix was analyzed by FTIR and XRD. Results indicated that the cross-linking effects between SPS and pectin matrix improved the thermal stability, water resistance and light shielding ability of the films. The incorporation of SPS in the films scavenged more than 80% of DPPH and ABTS free radicals, exhibited sustained antimicrobial ability against S. aureus, E. coli and B. cinerea, and showed significant color changes as pH-responsive films. Especially, the intelligent active coating/films inhibited the quality deterioration of cherry tomatoes and fresh-cut mangoes, and monitored the freshness of fresh-cut mangoes during storage. Therefore, the SPS/PE films have a potential application in maintaining fruit quality and monitoring the freshness of fresh-cut fruit.

Enhanced antibacterial efficacy against antibiotic-resistant bacteria via nitric oxide-releasing ampicillin polymer substrates.

The emergence of antibiotic-resistant bacteria poses a pressing threat to global health and is a leading cause of healthcare-related morbidity and mortality. Herein, we report the fabrication of medical-grade polymers incorporated with a dual-action S-nitroso-N-acetylpenicillamine-functionalized ampicillin (SNAPicillin) conjugated molecule through a solvent evaporation process. The resulting SNAPicillin-incorporated polymer materials act as broad-spectrum antibacterial surfaces that improve the administration efficacy of conventional antibiotics through the targeted release of both nitric oxide and ampicillin. The polymer surfaces were characterized by scanning electron microscopy and static contact angle measurements. The nitric oxide (NO) release profile and diffusion of SNAPicillin from polymers were quantified using a chemiluminescence-based nitric oxide analyzer (NOA) and ultraviolet-visible (UV-vis) spectroscopy. As a result, the films had up to 2.96 × 10-7 mol cm-2 of total NO released within 24 hr. In addition, >79 % of the SNAPicillin reservoir was preserved in the polymers after 24 hr of incubation in the physiological environment, indicating their longer-term NO release ability and therapeutic window for antibacterial effects. The SNAPicillin-incorporated polymers reduced the viability of adhered bacteria in culture, with >95 % reduction found against clinically relevant strains of Staphylococcus aureus (S. aureus). Furthermore, SNAPicillin-modified surfaces did not elicit a cytotoxic effect toward 3T3 mouse fibroblast cells, supporting the material's biocompatibility in vitro. These results indicate that the complementary effects of NO-release and ampicillin in SNAPicillin-eluting polymers can enhance the properties of commonly infected medical device surfaces for antibacterial purposes.

Design and synthesis of etrasimod derivatives as potent antibacterial agents against Gram-positive bacteria.

The emergence of multidrug-resistant bacteria along with a declining pipeline of clinically useful antibiotics has led to the urgent need for the development of more effective antibacterial agents. Inspired by our recent report on the antibacterial activity of etrasimod, an immunomodulating drug candidate, we prepared a series of etrasimod derivatives by varying substituents on the phenyl ring, altering the central tricyclic aromatic ring, and modifying the carboxyl group. From this series of compounds, indole derivative 24f was identified as the most potent antibacterial compound, with the minimum inhibitory concentration (MIC) values between 2.5 and 10 µM against various Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), S. epidermidis and enterococci. Moreover, 24f exhibited rapid bactericidal activity against S. aureus, low toxicity and hemolytic activity, and a synergistic effect with gentamicin against S. aureus, MRSA, and Enterococcus faecalis. Furthermore, it was shown that neither etrasimod nor 24f affects S. aureus cell membranes. Importantly, 24f did not induce resistance in S. aureus, representing a significant improvement compared to etrasimod. Finally, the antibacterial activity of etrasimod and 24f against S. aureus and MRSA was confirmed in vivo in a Caenorhabditis elegans infection model. Taken together, our study highlights the value of etrasimod and its derivatives as potential antibacterial candidates for combating infections caused by Gram-positive bacteria.

Efficient self-cleaning and antibacterial ceramics with ultra-low doping and high exposure of silver.

The secondary bacterial infection of COVID-19 is known to contribute significantly to mortality rates. Silver (Ag)-based antibacterial ceramics have emerged as a prominent solution for daily antibacterial applications, aiming to minimize the reliance on disinfectants while safeguarding human health. However, the fabrication of Ag-based antibacterial ceramics with low Ag content, high dispersion, and high exposure still faces challenges. In this work, an innovative method was proposed to doping Ag nanoparticles (Ag NPs) into glass ceramics (GC) via a "melt-freeze" method, then efficient and stable Ag-doped antibacterial ceramics (GC-xAg@BiOCl) were fabricated through facile in-situ HCl etching GC. Results indicate that the low Ag content (0.03 mol%) and high dispersion of Ag NPs are fully exposed and anchored on the surface, and constructed Schottky junction Ag/BiOCl contributed to antibacterial and photocatalytic activity. The degradation rates of norfloxacin and methylene blue by GC-0.25Ag@BiOCl can reach 71.0% and 55.3% under visible light irradiation, respectively. Moreover, The GC-0.25Ag@BiOCl exhibited significant antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S. aureus), with E.coli at 7.3 log10 cfu/mL and S. aureus at 7.0 log10 cfu/mL completely inactivated under visible light irradiation. Additionally, the antibacterial mechanism and charge transfer mechanism were explored.

A novel DM9-containing protein 7 involved in regulating the expression of CgMyD88 and CgIL-17 in oyster Crassostrea gigas.

The DM9-containing proteins have been identified as pattern recognition receptors (PRRs) to recognize invading pathogens and subsequently mediate downstream signal pathways, playing essential roles in innate immune responses of molluscs. In the present study, a novel DM9-containing protein (named as CgDM9CP-7) was identified from Pacific oyster Crassostrea gigas, which contained two tandem DM9 repeats similar to the previously identified CgDM9CPs. The mRNA transcripts of CgDM9CP-7 were found to be constitutively expressed in all the tested tissues including haemolymph, gill, hepatopancreas, mantle, adductor muscle and labial palp. The expression level of CgDM9CP-7 mRNA in haemocytes significantly up-regulated at 3 and 6 h after Vibrio splendidus stimulation, which was 5.67-fold (p < 0.01) and 4.71-fold (p < 0.05) of that in the control group, respectively, and it also increased significantly at 6 h (3.08-fold, p < 0.01) post lipopolysaccharide (LPS) stimulation. The protein of CgDM9CP-7 was mainly detected in membrane and cytoplasm of oyster haemocytes after V. splendidus stimulation. The recombinant CgDM9CP-7 protein (rCgDM9CP-7) displayed binding activities to MAN, LPS, PGN, Poly (I:C) as well as gram-negative bacteria (V. splendidus and Escherichia coli), gram-positive bacteria (Staphylococcus aureus and Micrococcus luteus) and fungi (Pichia pastoris and Yarrowia lipolytica). rCgDM9CP-7 was able to agglutinate Bacillus subtilis, V. splendidus, E. coli and S. aureus, inhibit their growth, and bind the recombinant protein CgMyd88-2 (KD = 5.98 × 10-6 M) and CgMyd88s (KD = 8.5 × 10-7 M) in vitro as well. The transcripts of CgIL17-1 (0.45-fold of the control group, p < 0.01), CgIL17-2 (0.19-fold, p < 0.05), CgIL17-3 (0.54-fold, p < 0.05), CgIL17-5 (0.36-fold, p < 0.05) and CgIL17-6 (0.24-fold, p < 0.01) in CgDM9CP-7-siRNA oysters decreased significantly at 6 h after V. splendidus stimulation. These results collectively indicated that CgDM9CP-7 was involved in the regulation of CgMyD88 and CgIL-17 expression in the immune response of oyster.

Carboxylated nanofibrillated cellulose empowers moxifloxacin to overcome Staphylococcus aureus biofilm in bacterial keratitis.

Bacterial keratitis is one of the vision-threatening ocular diseases that is increasing at an alarming rate due to antimicrobial resistance. One of the primary causes of antimicrobial resistance could be biofilm formation, which alters the mechanism and physiology of the microorganisms. Even a potent drug fails to inhibit biofilm due to the extracellular polysaccharide matrix surrounding the bacteria, inhibiting the permeation of drugs. Therefore, we aimed to develop carboxylated nanocellulose fibers loaded with moxifloxacin (Mox-cNFC) as a novel drug delivery system to treat bacterial corneal infection. Nanocellulose fibers were fabricated using a two-step method involving citric acid hydrolysis followed by TEMPO oxidation to introduce carboxylated groups (1.12 mmol/g). The Mox-cNFC particles showed controlled drug release till 40 h through diffusion. In vitro biofilm inhibition studies showed the particle's ability to disrupt the biofilm matrix and enhance the drug penetration to achieve optimal concentrations that inhibit the persister cells (without increasing minimum inhibitory concentration), thereby reducing the bacterial drug-resistant property. In vivo studies revealed the therapeutic potential of Mox-cNFC to treat Staphylococcus aureus-induced bacterial keratitis with once-a-day treatment, unlike neat moxifloxacin. Mox-cNFC could improve patient compliance by reducing the frequency of instillation and a controlled drug release to prevent toxicity.

Sonocatalytic induced dye degradation and antibacterial performance of SrTiO3 nanoparticles embedded cotton fabric.

In the present work, a pigment paste was prepared by adding strontium titanate (SrTiO3) nanoparticles (NPs) particles to a water and adhesive binder paste. Screen printing was utilized to embed the cotton fabric with the prepared pigment paste. Sonocatalytic induced antibacterial and dye mineralization abilities were evaluated for the printed fabric. The produced samples were examined for efficacy against the pathogens E. coli and S. aureus. The prepared SrTiO3 embedded cotton fabric inhibited (after 2 h) E. coli and S. aureus by 99.3% and 96.09%, respectively. The coated fabric was able to reduce pathogens by more than 92% even after 15 washing cycles. The Rhodamine B (RhB) dye was mineralized by 53% in 210 min by STO printed fabric as opposed to about 8% by pristine cotton. The results revealed that the intrinsic properties of cotton including tensile, abrasion, and air permeability remained unaffected by the printing of STO-NPs onto fabric.

Fabrication of next-generation multifunctional LBG-s-AgNPs@ g-C3N4 NS hybrid nanostructures for environmental applications.

Toxic industrial wastes and microbial pathogens in water pose a continuous threat to aquatic life as well as alarming situations for humans. Developing advanced materials with an environmentally friendly approach is always preferable for heterogeneous visible light photocatalysis. As a green reducing tool, LBG-s-AgNPs@ g-C3N4 NS hybrid nanostructures were anchored onto graphitic carbon nitride (g-C3N4) using an environmentally friendly approach of anchoring/decorating AgNPs onto g-C3N4. With the help of advanced techniques, the fabricated hybrid nanostructures were characterized. Using a sheet like matrix of g-C3N4, nanosized and well-defined uniform AgNPs displayed good antibacterial activity as well as superior photodegradation of hazardous dyes, including methylene blue (MB) and Rhodamine B (RhB). Based on the disc diffusion method, three pathogenic microorganisms of clinical significance can be identified by showing the magnitude of their susceptibility. As a result, the following antimicrobial potency was obtained: E. coli ≥ M. luteus ≥ S. aureus. In this study, green synthesized (biogenic) AgNPs decorated with g-C3N4 were found to be more potent antimicrobials than traditional AgNPs. Under visible light irradiation, LBG-s-AgNPs@g-C3N4 NS (0.01 M) demonstrated superior photocatalytic performance: ∼100% RhB degradation and ∼99% of MB degradation in 160 min. LBG-s-AgNPs@g-C3N4 NS showed the highest kinetic rate, 3.44 × 10-2 min-1, which is 27.74 times for the control activity in case of MB dye. While in case of RhB dye LBG-s-AgNPs@g-C3N4 NS showed the highest kinetic rate, 2.26 × 10-2 min-1, which is 17.51 times for the control activity. Due to the surface plasmon resonance (SPR) and reduction in recombination of the electrons and holes generated during photocatalysis, anchoring AgNPs to g-C3N4 further enhanced the photocatalytic degradation of dyes. Using this photocatalyst, hazardous dyes can be efficiently and rapidly degraded, allowing it to be applied for wastewater treatment contaminated with dyes. It also showed remarkable antimicrobial activity towards Gram-ve/Gram + ve pathogens.

Porphyrin-based covalent organic polymers with customizable photoresponses for photodynamic inactivation of bacteria.

Porphyrin-linked covalent organic polymers (COPs) provide a reliable photocatalytic platform, while photodynamic inactivation (PDI) induced by reliable porphyrin-based COPs is considered to be an effective method to resist microbial contamination. Herein, three tunable porphyrin-based covalent organic polymers (H2-Por-COPs, OH-Por-COPs, and Zn-Por-COPs) are designed and employed for the PDI of Staphylococcus aureus and Escherichia coli under visible light illumination. Interestingly, singlet oxygen (1O2) generation by the Por-COPs can be manipulated via intramolecular regulation with the order Zn-Por-COP > OH-Por-COP > H2-Por-COP. With rationally tune, the Zn-Por-COP demonstrated remarkable antibacterial activity against Staphylococcus aureus (kill percentage 99.65 % ± 0.24 %) and Escherichia coli (kill percentage 97.25 % ± 1.78 %) in only 15 min under visible-light irradiation. Density functional theory (DFT) calculations and photophysical tests showed that the presence of electron-donating -OH groups on the aromatic linkers and Zn2+ ions in porphyrin units narrowed the HOMO-LUMO gap, enhancing both light absorption, intersystem crossing (ISC) and 1O2 generation for more efficient bacteria inactivation. This work can be applied to efficiently screen suitable photosensitizers and provides a rational regulatory strategy for PDI of pathogenic bacteria.

Adaptive evolution of the Clf-Sdr subfamily contributes to Staphylococcus aureus musculoskeletal infection: Evidence from comparative genomics.

Persistent Staphylococcus aureus infections of the musculoskeletal system are a challenge in clinical practice. Although extensive studies on the genotypic changes in S. aureus in soft tissue and blood system infections have been conducted, little is known about how S. aureus adapts to the microenvironment of the musculoskeletal system. Here, we used comparative genomics to analyze the isolates from patients with an S. aureus infection of the musculoskeletal system. We observed that mutations in the Clf-Sdr subfamily proteins frequently occurred during persistent infections. Furthermore, these mutations were primarily located in the non-active site (R region), rather than in the active site (A region). Mechanistically, the clfA/B mutation enhanced the S. aureus biofilm formation ability through the binding to fibrinogen and intercellular adhesion. Complementation studies using the USA300-ΔMSCRAMMs strains clfA and clfB revealed that mutations in both the A and R regions could enhance their corresponding function. The results of protein structure prediction and ligand-binding simulations suggest that these mutations influence the protein structure and ligand binding. In conclusion, our study suggests that the Clf-Sdr subfamily mutations may be one of the mechanisms contributing to persistent S. aureus infections of the musculoskeletal system.

Estimation of the contribution to intraoperative pathogen transmission from bacterial contamination of patient nose, patient groin and axilla, anesthesia practitioners' hands, anesthesia machine, and intravenous lumen.

BACKGROUND: Earlier studies showed net cost saving from anesthesia practitioners' use of a bundle of infection prevention products, with feedback on monitored Staphylococcus aureus intraoperative transmission. ESKAPE pathogens also include Enterococcus and gram-negative pathogens: Klebsiella, Acinetobacter, Pseudomonas, and Enterobacter. We evaluated whether bacterial contamination of patient nose, patient groin and axilla, anesthesia practitioners' hands, anesthesia machine, and intravenous lumen all contribute meaningfully to ESKAPE pathogen transmission within anesthesia work areas. METHODS: The retrospective cohort study used bacterial count data from nine hospitals, 43 months, and 448 ESKAPE pathogen transmission events within anesthesia areas of 86 operating rooms. Transmission was measured within and between pairs of successive surgical cases performed in the same operating room on the same day. RESULTS: There were 203 transmission events with S. aureus, 72 with Enterococcus, and 173 with gram negatives. ESKAPE pathogens in the nose contributed to transmission for 50% (99% confidence limit ≥45%) of case pairs, on the groin or axilla for 54% (≥49%), on the hands for 53% (≥47%), on the anesthesia machine for 21% (≥17%), and in the intravenous lumen for 24% (≥20%). ESKAPE pathogens in the nose started a transmission pathway for 27% (≥22%) of case pairs, on the groin or axilla for 24% (≥19%), on the hands for 38% (≥33%), on the anesthesia machine for 11% (≥7.6%), and in the intravenous lumen for 8.0% (≥5.3%). All P ≤ 0.0022 compared with 5%. CONCLUSIONS: To prevent intraoperative ESKAPE pathogen transmission, anesthesia practitioners would need to address all five categories of infection control approaches: nasal antisepsis (e.g., povidone-iodine applied the morning of surgery), skin antisepsis (e.g., chlorhexidine wipes), hand antisepsis with dispensers next to the patient, decontamination of the anesthesia machine before and during anesthetics, and disinfecting caps for needleless connectors, disinfecting port protectors, and disinfecting caps for open female Luer type connectors.

Time evolution of electrical impedance spectra of Staphylococcus aureus and Escherichia coli bacteria.

This research work reports the time evolution of the electrical properties of gram-positive and gram-negative bacteria in aqueous suspensions with methyl violet and Lugol; measurements of galvanostatic electrical impedance spectra were made in a frequency range of 10Hz to 100kHz. The magnitude of the impedance as a function of frequency for methicillin-resistant strains, Staphylococcus aureus (gram-positive), and Escherichia coli O157: H7 (gram-negative) in the presence of methyl violet and Lugol, showed that both strains exhibited a progressive decrease in the magnitude of the electrical impedance with an increasing bacterial population; however, the variation in the magnitude rate of the impedance over time is completely different between the gram-positive and gram-negative strains. The results suggest that the time evolution of the electrical impedance spectra can be used to differentiate Staphylococcus aureus from Escherichia coli bacteria.

Natural antibacterial membranes prepared using Schisandra chinensis extracts and polyvinyl alcohol in an environment-friendly manner.

Foodborne pathogens can cause food spoilage and lead to food safety issues. In recent years, food packaging has received a lot of attention. Traditional packaging membranes are non-biodegradable and remain in the environment for a long time. In this study, natural antimicrobial substances were extracted from Schisandra chinensis by a green extraction process using distilled water as the solvent, and the effects of different treatment on the antimicrobial activity of the extract were compared. At the same time, four types of Schisandra chinensis antimicrobial membranes were prepared using polyvinyl alcohol (PVA) as the substrate. The whole extraction and membrane preparation process did not involve organic solvents, making the process green and environment friendly. Material characterization included inverted biological microscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), tensile strength test, pore size measurement, water uptake test, etc. Among them, no extract particles were observed with the naked eye on the surfaces of MⅡ and MⅣ. MⅡ has a uniformly transparent, nearly colorless morphology and is the most tensile. MⅣ surface is flat and smooth, the microstructure is dense and uniform. At the same time, the four types of membranes were tested against common pathogenic bacteria for 12 h, and the OD600 trend revealed the excellent antimicrobial activity of the membranes against S. aureus, MRSA, E. coli, and L. monocytogenes. The membranes could also be reused at least once. This study provides a new idea for preparing natural plant-based antimicrobial membranes.

Constructing a novel type-â ¡ ZnO/BiOCOOH heterojunction microspheres for the degradation of tetracycline and bacterial inactivation.

A novel ZnO/BiOCOOH microsphere photocatalyst with a type-Ⅱ mechanism was developed for the first time. This strategy was accomplished by immobilizing ZnO onto 3D BiOCOOH microspheres via a single-step hydrothermal synthesis method. The ability to degrade tetracycline (TC) in water under visible light and inactivate bacteria of as-catalyst were analyzed. Among the prepared samples, the ZnO/BiOCOOH composite, with a mass ratio of 40%(Zn/Bi), exhibited the highest photocatalytic activity, which was able to degrade 98.22% of TC in just 90 min and completely eradicated Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in 48 h, and had potential application in solving water resource environmental pollution. The photoelectric characteristics of the photocatalysts were examined by means of electrochemical impedance spectroscopy (EIS) and photoluminescence (PL) spectroscopy. The findings indicated that the superior photocatalytic performance could be credited to the dissociation of electrons (e-) and holes (h+) in heterojunction composites. Finally, electron paramagnetic resonance (EPR) and capture experiments were conducted to confirm the photocatalytic mechanism of the type-Ⅱ heterojunction. This work provides a new Bi-base photocatalyst for aqueous environmental control.

Investigating synergism and mechanism during sequential inactivation of Staphylococcus aureus with ultrasound followed by UV/peracetic acid.

This study explored the inactivation of Staphylococcus aureus (S. aureus) by ultrasound (US) and peracetic acid (PAA) coupling with UV simultaneously (US/PAA/UV) or sequentially (US→PAA/UV) for the strengthened disinfection. The result showed that US→PAA/UV system had excellent inactivation performance with 5.05-log in a short time. Besides US, UV, PAA and free radicals, the contribution of the synergy of all components to the entire disinfection were obvious under US→PAA/UV system. The inactivation performance of S. aureus significantly decreased with the increase of humic acid (HA) concentration and pH; however, the rising temperature contributes to the enhancement of the inactivation efficiency under the US→PAA/UV system. The disinfection mechanism includes a decrease of cell agglomeration, a loss of intracellular substance, and changes of cell structure and membrane permeability, as evidenced through a nanoparticle size analyzer, scanning electron microscope (SEM), transmission electron microscope (TEM) and laser confocal microscopy (LSCM). Furthermore, the inactivation efficiency of the US→PAA/UV system for the total bacteria from actual sewage (the untreated inflow) was high, which reached 3.86-log. In general, the pretreatment of US combined with UV/PAA showed a promising application in the rapid disinfection of sewage.

The Application of Bacteriophage and Photoacoustic Flow Cytometry in Bacterial Identification.

Infection with resistant bacteria has become an ever-increasing problem in modern medical practice. Bacteremia is a serious and potentially lethal condition that can lead to sepsis without early intervention. Currently, broad-spectrum antibiotics are prescribed until bacteria can be identified through blood cultures, a process that can take 2-3 days and is unable to provide quantitative information. Staphylococcus aureus (S. aureus) is a leading cause of bacteremia, and methicillin-resistant S. aureus (MRSA) accounts for more than a third of the cases. Other bacteria such as Clostridium difficile, Acinetobacter baumannii, and Carbapenem-resistant Enterobacteriaceae are becoming more prevalent and antibiotic-resistant. Rapid diagnostics for each of these superbugs has been a priority for health organizations around the world. Bacteriophages have evolved for millions of years to develop exquisite specificity in target binding using their host attachment proteins. Bacteriophages are viruses that infect bacteria. Bacteriophages use tail spikes, specialized attachment proteins, to bind specifically to their target bacterial cell surface proteins. We use bacteriophages and parts of bacteriophages as specific tags coupled with photoacoustic flow cytometry for the detection and quantification of bacteria. In photoacoustic flow cytometry, laser light is absorbed by particles under flow, and the ultrasound waves generated on the release of the energy are detected. Photoacoustics involves the detection of ultrasound waves resulting from laser irradiation. In photoacoustic flow cytometry, pulsed laser light is delivered to a sample flowing past a focused transducer, and particles that absorb laser light create a photoacoustic response. Bacteria can be tagged with dyed bacteriophage and processed through a photoacoustic flow cytometer where they are detected by the acoustic response. In this chapter, we describe the procedure and methods used to accomplish this. Often the limiting factor for the treatment of patients is the time spent waiting for results. It is our hope that the work presented in this chapter can be a foundation for future work and provide an ability to detect bacterial pathogens in blood cultures. Bacterial plate cultures and Gram staining are nineteenth-century technologies that have been the gold standards for decades, but current trends in resistant bacteria have necessitated a move toward more rapid and quantifiable diagnostic tools.