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1.
Chem Soc Rev ; 49(4): 1144-1172, 2020 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-31971181

RESUMO

The emergence of aggregation-induced emission luminogens (AIEgens) has significantly stimulated the development of luminescent supramolecular materials because their strong emissions in the aggregated state have resolved the notorious obstacle of the aggregation-caused quenching (ACQ) effect, thereby enabling AIEgen-based supramolecular materials to have a promising prospect in the fields of luminescent materials, sensors, bioimaging, drug delivery, and theranostics. Moreover, in contrast to conventional fluorescent molecules, the configuration of AIEgens is highly twisted in space. Investigating AIEgens and the corresponding supramolecular materials provides fundamental insights into the self-assembly of nonplanar molecules, drastically expands the building blocks of supramolecular materials, and pushes forward the frontiers of supramolecular chemistry. In this review, we will summarize the basic concepts, seminal studies, recent trends, and perspectives in the construction and applications of AIEgen-based supramolecular materials with the hope to inspire more interest and additional ideas from researchers and further advance the development of supramolecular chemistry.


Assuntos
Substâncias Luminescentes/química , Imagem Óptica , Nanomedicina Teranóstica , DNA/química , Portadores de Fármacos/química , Humanos , Compostos Macrocíclicos/química , Microscopia Confocal , Neoplasias/diagnóstico por imagem , Peptídeos/química
2.
Talanta ; 207: 120344, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594624

RESUMO

Glyphosate (Gly) is the most widely used herbicide at the moment. It presents a broad spectrum of action, hence its use for many different crops. Regulatory agencies have constantly mentioned the low hazard potential of Gly to mammals. However, the International Agency for Research on Cancer concluded in 2015 that glyphosate is "probably carcinogenic to humans". For this reason, it is important to develop reliable analytical methods to quantify Gly in food samples. Here, we propose an analytical method that makes use of graphene quantum dots (GQDs) and cysteine-capped silver nanoparticles (AgNPs) for the screening of glyphosate, using QuEChERS as sample treatment. Gly quenched the luminescence of GQDs-AgNPs system, achieving an excellent sensitivity (detection limit of 9 ng mL-1) and selectivity. The method developed was applied to different types of pulses (peas and lupins), obtaining recoveries close to 100% and relative standard deviations lower than 4% in all cases. Its simplicity and rapidity make this method an interesting alternative to other existing methodologies for the analysis of this pesticide in food samples.


Assuntos
Análise de Alimentos/métodos , Glicina/análogos & derivados , Grafite/química , Substâncias Luminescentes/química , Nanopartículas Metálicas/química , Pontos Quânticos/química , Prata/química , Glicina/análise , Glicina/química , Limite de Detecção
3.
Talanta ; 206: 120206, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514889

RESUMO

Hemoglobin (Hb) plays an important role in oxygen carriage for mammals, which is also a typical biomarker for certain diseases. Although numerous methods had been developed for the detection of Hb in red blood cells, analytical technology for the monitoring of low-abundance Hb in serum or plasma is still a challenge. Herein, persistent luminescence nanoparticles (PLNPs) with strong near-infrared (NIR) emission character behaving as a label-free probe for the highly sensitive and selective detection of Hb were developed. Further studies revealed that the sensing mechanism should be attributed to the Hb-induced dynamic quenching process. Moreover, the nanoprobe showed high selectivity to Hb against the common existing substances in human serum and a linear response to Hb ranging from 1 to 50 nM with an extremely high limit of detection (LOD) of 0.13 nM. Finally, applicability of the proposed probe for the detection of Hb in human serum samples was validated.


Assuntos
Hemoglobinas/análise , Substâncias Luminescentes/química , Nanopartículas Metálicas/química , Cromo/química , Gálio/química , Humanos , Limite de Detecção , Luminescência , Medições Luminescentes/métodos , Tamanho da Partícula , Zinco/química
4.
Inorg Chem ; 58(21): 14522-14531, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31550141

RESUMO

Herein we report the synthesis, characterization, and cellular internalization properties of two visible-light active luminescent Mn-based photoCORMs. The enhanced membrane permeability of the photoactive Mn carbonyl complex (photoCORM) derived from a designed lipophilic ligand namely, [Mn(CO)3(Imdansyl)(L1)](CF3SO3) (1) (where L1 = a diazabutadiene-based ligand containing two highly lipophilic adamantyl motifs, Imdansyl = dansylimidazole) promoted rapid internalization within human colorectal adenocarcinoma (HT-29) cells compared to [Mn(CO)3(Imdansyl)(L2)](CF3SO3) (2) (where L2 = a diazabutadiene ligand bearing two hydrophilic 1,3,5-triazaadamantyl group). Colocalization experiments using membrane stain indicate different extents of localization of the two CO complexes within the cellular matrix. Visible-light triggered CO release from the lipophilic photoCORM induced caspase-3/7 activation on HT-29 cells, which was detected using confocal microscopy. The rapid accumulation of the lipophilic photoCORM 1 in the cellular membrane resulted in more efficient CO-induced cell death compared to the hydrophilic analogue 2.


Assuntos
Complexos de Coordenação/farmacologia , Luz , Substâncias Luminescentes/farmacologia , Morte Celular/efeitos dos fármacos , Complexos de Coordenação/síntese química , Complexos de Coordenação/química , Cristalografia por Raios X , Células HT29 , Humanos , Interações Hidrofóbicas e Hidrofílicas , Ligantes , Substâncias Luminescentes/síntese química , Substâncias Luminescentes/química , Modelos Moleculares , Neoplasias/tratamento farmacológico , Solubilidade
5.
Inorg Chem ; 58(19): 12700-12706, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31556291

RESUMO

The highly porous luminescent metal-organic frameworks (MOFs) can act as fluorescent probes for the detection of nitro compounds and can also serve as containers and energy transfer platforms to construct the host-guest systems. Herein, two new three-dimensional MOFs with high porosity were prepared successfully by the electron-rich tetrakis(4-pyridylphenyl)ethylene (tppe) as ligands. Compound 1 shows the high sensitivity and selectivity toward nitro-antibiotics in an aqueous media, particularly showing the best detection efficiency for furazolidone (FZD) among the reported luminescent sensors. The highly efficient fluorescence quenching toward FZD may be attributed to the electron and energy transfer. Compound 2 has naphthalene-2,7-dicarboxylic acid (2,7-npd) and tppe as dual linkers, and the energy transfer between 2,7-npd and tppe leads to the emission band in a large scale. It is worth noting that the single-phased white-light materials can be obtained by the in situ encapsulation of different concentration of sulforhodamine 101 (SR101) into compound 2 matrix.


Assuntos
Antibacterianos/análise , Substâncias Luminescentes/química , Estruturas Metalorgânicas/química , Nitrocompostos/análise , Estilbenos/química , Cristalografia por Raios X , Luminescência , Medições Luminescentes , Modelos Moleculares , Rodaminas/química
6.
Nat Commun ; 10(1): 3996, 2019 09 05.
Artigo em Inglês | MEDLINE | ID: mdl-31488822

RESUMO

On-command changes in the emission color of functional materials is a sought-after property in many contexts. Of particular interest are systems using light as the external trigger to induce the color changes. Here we report on a tri-component cocktail consisting of a fluorescent donor molecule and two photochromic acceptor molecules encapsulated in polymer micelles and we show that the color of the emitted fluorescence can be continuously changed from blue-to-green and from blue-to-red upon selective light-induced isomerization of the photochromic acceptors to the fluorescent forms. Interestingly, isomerization of both acceptors to different degrees allows for the generation of all emission colors within the red-green-blue (RGB) color system. The function relies on orthogonally controlled FRET reactions between the blue emitting donor and the green and red emitting acceptors, respectively.


Assuntos
Cor , Transferência Ressonante de Energia de Fluorescência/métodos , Substâncias Luminescentes/química , Fótons , Corantes , Técnicas Eletroquímicas , Fluorescência , Espectrometria de Fluorescência/métodos
7.
Biosens Bioelectron ; 142: 111551, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31382095

RESUMO

This work reported a ternary quenching strategy based on electrochemiluminescence resonance energy transfer (ECL-RET) between luminol/H2O2 system and MnO2 capped carbon nanospheres (MnO2@C) and Mn2+ released from the reduction of MnO2 and ascorbic acid (AA) consumed superoxide radical (O2•-) for detection of insulin. To improve the ECL behavior of luminol, AuPdPt hybrid nanoparticles decorated MoS2 nanosheet-coated TiO2 nanobelt (AuPdPt-MoS2@TiO2) were applied to combine with luminol and primary antibodies via covalent linkage which exhibited perfectly catalysis for the electroreduction of H2O2. In order to sensitively detect insulin, labels MnO2@C were used to anchor secondary antibodies via electrostatic interaction and inhibit the ECL intensity of luminol. The perfect overlap of the ECL spectrum of luminol with the absorption spectrum of MnO2 allowed the ECL-RET to decrease ECL signals of luminol. Specifically, AA was introduced not only to inhibit the ECL of luminol, but also to reduce MnO2 producing Mn2+ which can be reacted with O2•- to further quench the ECL reaction between luminol and H2O2. On the basis of the dual quenching effect of luminol, the sandwich immunosensor was prepared to determine insulin with wide linear range from 0.5 pg mL-1 to 60 ng mL-1 and a low detection limit of 0.13 pg mL-1 (S/N = 3).


Assuntos
Insulina/sangue , Medições Luminescentes/métodos , Luminol/química , Compostos de Manganês/química , Nanosferas/química , Óxidos/química , Ácido Ascórbico/química , Técnicas Biossensoriais/métodos , Carbono/química , Humanos , Imunoensaio/métodos , Limite de Detecção , Substâncias Luminescentes/química
8.
Adv Mater ; 31(39): e1902469, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31402525

RESUMO

Cells transport mass dynamically, crossing cell membranes to maintain metabolism and systemic homeostasis, through which biomolecules are also delivered to cells for gene editing, cell reprograming, therapy, and other purposes. Quantifying the translocation kinetics is fundamentally and clinically essential, but remains limited by fluorescence-based technologies, which are semi-quantitative and only provide kinetics information at cellular level or in discrete time. Herein, a real-time method of quantifying cell internalization kinetics is reported using functionalized firefly-luciferase nanocapsules as the probe. This quantitative assay will facilitate the rational design of delivery vectors and enable high-throughput screening of peptides and other functional molecules, constituting an effective tool for broad applications, including drug development and cancer therapy.


Assuntos
Luciferases de Vaga-Lume/química , Luciferases de Vaga-Lume/metabolismo , Substâncias Luminescentes/química , Substâncias Luminescentes/metabolismo , Nanocápsulas/química , Animais , Linhagem Celular Tumoral , Cinética , Camundongos , Transporte Proteico
9.
Nat Commun ; 10(1): 3735, 2019 08 19.
Artigo em Inglês | MEDLINE | ID: mdl-31427582

RESUMO

Triplet energy transfer occurs frequently in natural photosynthetic organisms to protect against photo-oxidative stress. For artificial light-harvesting systems, several challenges need to be addressed to realize triplet energy transfer especially in aqueous medium. Specifically, the phosphors should be shielded from water and molecular oxygen, which facilitate to maintain intense emission intensity. Moreover, the donor‒acceptor phosphors should be organized in close proximity, yet simultaneously avoiding direct homo- and hetero-interactions to minimize the potential energy losses. Herein an effective strategy has been developed to meet these requirements, by employing a rod-coil amphiphile as the compartmentalized agent. It renders synergistic rigidifying and hydrophobic shielding effects, giving rise to enhanced phosphorescent emission of the platinum(II) complexes in aqueous environment. More importantly, the donor‒acceptor platinum(II) phosphors feature ordered spatial organization in the ternary co-assembled system, resulting in high light-harvesting efficiency. Therefore, the compartmentalization strategy represents an efficient approach toward color-tunable phosphorescent nanomaterials.


Assuntos
Transferência de Energia/fisiologia , Substâncias Luminescentes/química , Nanoestruturas/química , Luz , Complexos de Proteínas Captadores de Luz/química , Medições Luminescentes , Modelos Moleculares , Oxigênio/química , Fotossíntese , Platina/química , Água/química
10.
Dalton Trans ; 48(36): 13536-13540, 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31455966

RESUMO

Selective detection of DNA defects is an attractive approach for early detection of various tumors and diseases. Ru(ii), Rh(iii) and hybrid complexes have been reported in this regard. Here, we present three heteroleptic cyclometalated Ir(iii) complexes [Ir(phpy)2(imiphen)]+(Ir-1), [Ir(phpy)2(furphen)]+(Ir-2) and [Ir(phpy)2(faqphen)]+(Ir-3) where imiphen: 2-(1H-imidazol-2-yl)-1H-imidazo[4,5-f][1,10]phenanthroline; furphen: 2-(furan-2-yl)-1H-imidazo[4,5-f][1,10]phenanthroline and faqphen: 2-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)anthracene-9,10-dione have been synthesized and characterized by analytical techniques. All three complexes recognize DNA defects with high selectivity as Ir-1 recognizes the abasic site opposite T and CA mismatch, Ir-2 recognizes Ab : G and Ir-3 recognizes the TT mismatch preferably. The results from this study further emphasize that the development of new luminescent probes requires fine-tuning of the ligand structure and the choice of the metal center to improve selectivity in recognition of DNA defects.


Assuntos
Dano ao DNA , DNA/química , DNA/genética , Substâncias Luminescentes/química , Compostos Organometálicos/química , Rutênio/química
11.
Analyst ; 144(16): 4813-4819, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31281909

RESUMO

In the clinical diagnosis of tumor, the immunological detection of single tumor markers may lead to errors and missed inspection. Therefore, it is necessary to establish an accurate and effective method for the simultaneous detection of multiple tumor markers. Thus, we developed a time-resolved chemiluminescence immunoassay (TRCLIA) to simultaneously detect carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE) in human serum. Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) were used as the detection probes to label the monoclonal antibodies of CEA and NSE by strain-promoted azide-alkyne cycloaddition (SPAAC), respectively. Based on a sandwich immunoassay, the targets in the samples were captured by antibodies immobilized on the surface of carboxylate-modified polystyrene microspheres (CPSMS) and sandwiched by other antibodies labeled with HRP and ALP. Since HRP and ALP had different dynamic characteristics, the CEA and NSE signals were recorded at 0.5 s and 20 min, respectively, and cross-interference could be avoided effectively. The whole signal detection processes could be completed in 20 min. The linear ranges of CEA and NSE were 0.1-64 ng mL-1 and 0.05-64 ng mL-1 and the limits of detection were 0.085 ng mL-1 and 0.044 ng mL-1 (S/N = 2), respectively. Also, 45 human serum samples obtained from patients having lung disease were tested by TRCLIA and commercial chemiluminescence enzyme-linked immunoassay (CLEIA) kits with good correlation. The correlation coefficients of CEA and NSE were 0.985 and 0.970, respectively. The results demonstrated a novel, effective, reliable and convenient TRCLIA method for the clinical diagnosis of CEA and NSE. The TRCLIA method has the potential to be an effective clinical tool for the early screening of lung cancer and can be applied in clinical diagnosis.


Assuntos
Antígeno Carcinoembrionário/sangue , Técnicas Imunoenzimáticas/métodos , Fosfopiruvato Hidratase/sangue , Fosfatase Alcalina/química , Anticorpos Imobilizados/imunologia , Anticorpos Monoclonais/imunologia , Armoracia/enzimologia , Biomarcadores Tumorais/sangue , Biomarcadores Tumorais/imunologia , Antígeno Carcinoembrionário/imunologia , Peroxidase do Rábano Silvestre/química , Humanos , Limite de Detecção , Luminescência , Substâncias Luminescentes/química , Medições Luminescentes/métodos , Luminol/química , Pneumopatias/sangue , Fosfopiruvato Hidratase/imunologia
12.
J Colloid Interface Sci ; 554: 520-530, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31330425

RESUMO

A one-pot simple procedure for the synthesis of uniform, ellipsoidal Eu3+-doped sodium lanthanum tungstate and molybdate (NaLa(XO4)2, X  = W, Mo) nanophosphors, functionalized with carboxylate groups, is described. The method is based on a homogeneous precipitation process at 120 °C from appropriate Na+, Ln3+ and tungstate or molybdate precursors dissolved in ethylene glycol/water mixtures containing polyacrylic acid. A comparative study of the luminescent properties of both luminescent materials as a function of the Eu3+ doping level has been performed to find the optimum nanophosphor, whose efficiency as X-ray computed tomography contrast agent is also evaluated and compared with that of a commercial probe. Finally, the cell viability and colloidal stability in physiological pH medium of the optimum samples have also been studied to assess their suitability for biomedical applications.


Assuntos
Meios de Contraste/química , Európio/química , Lantânio/química , Substâncias Luminescentes/química , Molibdênio/química , Compostos de Tungstênio/química , Animais , Meios de Contraste/síntese química , Substâncias Luminescentes/síntese química , Tomografia Computadorizada por Raios X , Compostos de Tungstênio/síntese química , Células Vero
13.
Dalton Trans ; 48(32): 12257-12271, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31339136

RESUMO

Of late, cancer has become a terrible disease affecting people throughout the world. Keeping this in mind, we tried to design drugs that are more lipophilic, target-specific, water-soluble, cytoselective and fluorescent. In this regard, we reported novel ruthenium(ii)-p-cymene imidazophenanthroline scaffolds as effective DNA targeting agents. The planarity of imidazophenanthroline ligands caused the Ru(ii) complex to be a good intercalator. An extended π-electronic conjugation was introduced in the imidazophenanthroline moieties through the Suzuki and Sonogashira coupling reactions. Here, we synthesized nine Ru(ii) complexes (16a-b, 17a-d, and 19a-c). Among these, [(η6-p-cymene)RuCl(K2-N,N-2-(4'-methyl-[1,1'-BIphenyl]-4-yl)-1H-imidazo[4,5-f][1,10]phenanthroline)]·PF6 (16b) exhibited the best potency and selectivity with excellent cellular uptake; [(η6-p-cymene)RuCl(K2-N,N-2-(4-(phenylethynyl)phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline)]·PF6 (17a) acted as a cytoselective probe for live cell imaging.


Assuntos
Antineoplásicos/análise , Complexos de Coordenação/análise , Substâncias Luminescentes/análise , Imagem Óptica , Fenantrolinas/análise , Rutênio/análise , Antineoplásicos/síntese química , Antineoplásicos/química , Sobrevivência Celular , Complexos de Coordenação/síntese química , Complexos de Coordenação/química , Relação Dose-Resposta a Droga , Células HeLa , Humanos , Ligantes , Substâncias Luminescentes/síntese química , Substâncias Luminescentes/química , Estrutura Molecular , Fenantrolinas/química , Rutênio/química , Relação Estrutura-Atividade
14.
Int J Mol Sci ; 20(14)2019 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-31336869

RESUMO

In this work, LaPO4:Ce, Tb phosphors were prepared by firing a LaPO4:Ce, Tb precipitate using an ionic-liquid-driven supported liquid membrane system. The entire system consisted of three parts: a mixed rare earth ion supply phase, a phosphate supply phase, and an ionic-liquid-driven supporting liquid membrane phase. This method showed the advantages of a high flux, high efficiency, and more controllable reaction process. The release rate of PO43- from the liquid film under different types of ionic liquid, the ratio of the rare earth ions in the precursor mixture, and the structure, morphology, and photoluminescence properties of LaPO4:Ce, Tb were investigated by inductively coupled plasma-atomic emission spectroscopy, X-ray diffraction, Raman spectra, scanning electron microscopy, and photoluminescence emission spectra methods. The results showed that a pure phase of lanthanum orthophosphate with a monoclinic structure can be formed. Due to differences in the anions in the rare earth supply phase, the prepared phosphors showed micro-spherical (when using rare earth sulfate as the raw material) and nanoscale stone-shape (when using rare earth nitrate as the raw material) morphologies. Moreover, the phosphors prepared by this method had good luminescent properties, reaching a maximum emission intensity under 277 nm excitation with a predominant green emission at 543 nm which corresponded to the 5D4-7F5 transition of Tb3+.


Assuntos
Cério/química , Líquidos Iônicos , Lantânio/química , Metais Terras Raras/química , Fosfatos/química , Fósforo/química , Luminescência , Substâncias Luminescentes/química , Estrutura Molecular , Difração de Raios X
15.
Talanta ; 204: 379-385, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31357309

RESUMO

Fabricating simple, accurate and user-friendly diagnostic device for "point of care testing" (POCT) applications is one of the most challenging objectives in the analytical field. Hemin detection is important for drugs monitoring, diagnosis, and forensic latent bloodstain imaging. Herein is developed, luminol chemiluminescence biosensor for hemin detection using artesunate as coreactant. A possible mechanism to account for the chemiluminescence reaction is discussed. Hemin was detected using both photomultiplier tube (PMT) and smartphone as detector. The detection limit for hemin using smartphone as detector is 20 nM, enabling the visual detection of hemin in blood sample with a dilution factor of blood up to 120,000. While PMT detector is used, the system is able to detect hemin down to 0.22 nM. In addition to high sensitivity, this sensing system exhibit high selectivity. It can successfully distinguish bloodstain from other stains while applying the system for point of care testing using smart phone as detector. Moreover, the system can detect artesunate with a linear range from 0.1 nM to 1.0 µM with a limit of detection of 0.078 nM.


Assuntos
Artesunato/química , Hemina/análise , Substâncias Luminescentes/química , Luminol/química , Artesunato/análise , Análise Química do Sangue/instrumentação , Análise Química do Sangue/métodos , Manchas de Sangue , Calibragem , Humanos , Limite de Detecção , Medições Luminescentes/instrumentação , Medições Luminescentes/métodos , Smartphone
16.
Anal Bioanal Chem ; 411(23): 6049-6056, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31280477

RESUMO

As an important glycoprotein of the lectin family, soybean agglutinin (SBA) is an anti-nutritional factor with considerable toxic and side effects and plays a significant role in tumor analysis. In order to achieve the sensitive detection of SBA, a sandwich-structured electrochemiluminescence (ECL) biosensor was constructed using carboxylated carbon nitride (C-g-C3N4) as luminophore and D-galactosamine (galM) as a recognition element. A glassy carbon electrode (GCE) was modified with Au nanoparticles (Au NPs) for capturing the galM via Au-N bond, and further capturing the target SBA by specific recognition between galM and SBA. In the presence of SBA, the composite C-g-C3N4-galM was immobilized onto the electrode. With the increase in the concentration of SBA, the ECL signal from C-g-C3N4 increased, thus achieving a signal-on detection of SBA. The linear range of the biosensor was 1.0 ng/mL~10 µg/mL and detection limit for SBA was as low as 0.33 ng/mL. In this construction strategy, C-g-C3N4 not only acted as an excellent signal probe, but also as an immobilization matrix to easily achieve a high loading of the small molecule recognition element galM. This strategy provides a simple alternative SBA detection platform. Graphical abstract.


Assuntos
Galactosamina/química , Grafite/química , Substâncias Luminescentes/química , Nitrilos/química , Lectinas de Plantas/análise , Proteínas de Soja/análise , Técnicas Biossensoriais/métodos , Ácidos Carboxílicos/química , Técnicas Eletroquímicas/métodos , Ouro/química , Humanos , Limite de Detecção , Medições Luminescentes/métodos , Nanopartículas Metálicas/química , Lectinas de Plantas/sangue , Proteínas de Soja/sangue
17.
Molecules ; 24(12)2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31213026

RESUMO

Three new organosilicon compounds based on dithienosilole (DTSi) were synthesized in good yields. We report the optical and electrochemical properties of the resulting derivatives. We find that these compounds absorb the light in the ultraviolet and blue light range, and they exhibit luminescence in almost the entire range of visible light. After electropolymerization were significantly lowered, the values of the energy gap (even 1.51 eV for P2) and the ionization potential of the polymers were compared to monomers. Optoelectronic properties of the obtained compounds suggest that these derivatives of DTSi may be good candidates as the emissive layers in white organic light-emitting diodes (WOLEDs), which would reduce the amount of layers.


Assuntos
Substâncias Luminescentes/química , Compostos de Organossilício/química , Técnicas de Química Sintética , Eletroquímica , Substâncias Luminescentes/síntese química , Estrutura Molecular , Compostos de Organossilício/síntese química , Análise Espectral
18.
Biosens Bioelectron ; 141: 111436, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31226604

RESUMO

The low-potential electrochemiluminescence (ECL) sensors based on cathodic light emission of luminol have caused more and more concerns due to their good stability and reproducibility. In this work, highly porous platinum (Pt) nanostructures on ionic liquid functionalized graphene film (GR-IL/pPt) were prepared as platform to construct a label-free ECL sensor for the detection of carcinoembryonic antigen (CEA). Due to their good biocompatibility, excellent electrocatalytic activity and highly porous structure, the as-prepared GR-IL/pPt composites benefited amplified cathodic ECL signal of luminol and high loading density of the CEA antibody. After CEA was incubated with the CEA antibody, the cathodic ECL signal of luminol decreased thanks to the less conductive immunocomplex. The proposed ECL immunosensor realized high sensitivity for CEA detection with a wide linear range from 0.001 fg mL-1 to 1 ng mL-1 and an extremely low detection limit of 0.0003 fg mL-1 (S/N = 3). Moreover, the sensor showed good specificity, stability and reproducibility, indicating that the provided strategy had a promising potential in clinical detection.


Assuntos
Técnicas Biossensoriais/métodos , Antígeno Carcinoembrionário/sangue , Substâncias Luminescentes/química , Medições Luminescentes/métodos , Luminol/química , Técnicas Eletroquímicas/métodos , Grafite/química , Humanos , Líquidos Iônicos/química , Limite de Detecção , Platina/química , Porosidade
19.
Biosens Bioelectron ; 141: 111438, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31254862

RESUMO

Development of a simple, cost-effective and sensitive biosensing strategy is highly desirable to advance the applications in Alzheimer's disease diagnosis. In this paper, we present a simple, label-free and signal-on electrochemiluminescence (ECL) aptasensor for the detection of amyloid-beta (Aß) peptide using luminol as ECL emitter and in-situ generated reactive oxygen species (ROS) as coreactant via catalytic reaction between Cu2+-Aß and the dissolved O2 in the presence of ascorbic acid (AA). Aß16, the binding site of Cu2+ in the monomeric full-length Aß, was used as a model in present study. As a result, this signal-on ECL aptasensor has exhibited favorable analytical performance for Aß16 monomer with a linear range of 1.0 × 10-13 mol/L-1.0 × 10-8 mol/L and a limit of detection of 3.5 × 10-14 mol/L (S/N=3). Furthermore, the proposed biosensor was also able to detect the full length Aß40 not only in the phosphate buffer saline (PBS) solution but also in human serum. The presented biosensor represents a promising, simple, turn-on and label-free diagnostic tool for blood analysis.


Assuntos
Peptídeos beta-Amiloides/sangue , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Fragmentos de Peptídeos/sangue , Peptídeos beta-Amiloides/análise , Cobre/química , Técnicas Eletroquímicas/métodos , Humanos , Limite de Detecção , Substâncias Luminescentes/química , Medições Luminescentes/métodos , Luminol/química , Fragmentos de Peptídeos/análise
20.
Biosens Bioelectron ; 141: 111450, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31247454

RESUMO

Nanozymes have fascinated increasing attention in the field of artificial enzyme. Designing an ideal nanozyme usually requires a synergic advantage of reasonable nanostructures and large specific surface area for ensuring excellent mimicking-enzyme catalytic activity. Here we report a CuS nanozyme with hollow nanocube structure (h-CuS NCs), which has a large surface area of 57.84 m2 g-1, and thus realizes excellent mimicking-enzyme catalytic activity. Expectedly, our directed design of h-CuS NCs nanozymes has an affinity for H2O2 of 0.94 mM, which is outstanding among the state-of-the-art Cu-based nanozymes. Furthermore, this nanozyme acts as a multifunctional catalyst to induce luminol chemiluminescence and oxide 3, 3', 5, 5'-tetramethylbenzidine (TMB) in the presence of H2O2, and displays distinguished electrocatalytic activity to glucose oxidation. More intriguingly, the nanozyme can produce a promising photothermal effect under the illumination of near-infrared light. This work will provide a prototype for rational design of distinct nanostructures as multifunctional nanozymes in the area of electrochemical sensing, mimicking-enzyme catalytic biosening and cancer therapy.


Assuntos
Colorimetria/métodos , Cobre/química , Dopamina/análise , Glucose/análise , Nanoestruturas/química , Sulfetos/química , Benzidinas/química , Técnicas Biossensoriais/métodos , Catálise , Técnicas Eletroquímicas/métodos , Peróxido de Hidrogênio/química , Substâncias Luminescentes/química , Luminol/química , Nanoestruturas/ultraestrutura , Oxirredução
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