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1.
Bioresour Technol ; 296: 122290, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31677404

RESUMO

Three-dimensional biofilm electrode reactors (3D-BERs) with high treatment efficiency were constructed to treat wastewater containing sulfadiazine (SDZ) and ciprofloxacin (CIP) coexposure with Zinc (Zn). The results showed that coexposure to target antibiotics and Zn increased the absolute and relative abundances of target antibiotic resistance genes (ARGs). Additionally, the target ARG abundances were higher on cathode of 3D-BER compared with ordinary anaerobic reactor while the abundances of total ARGs were decreased in the effluent. Meanwhile, redundancy analysis results revealed that the composition of bacteria carrying ARGs was greatly influenced in the cathode by the accumulation of Zn and antibiotic, which dominated the changes of ARG abundances. Additionally, ARGs with their host bacteria revealed by network analysis were partially deposited on electrode substrates when being removed from wastewater. Thus, 3D-BER exhibits capability of simultaneously eliminating antibiotic and Zn, and greatly reduces the risks of ARGs spread.


Assuntos
Antibacterianos , Ciprofloxacino , Bactérias , Biofilmes , Resistência Microbiana a Medicamentos , Eletrodos , Genes Bacterianos , Características de Residência , Sulfadiazina , Águas Residuárias , Zinco
2.
Bull Environ Contam Toxicol ; 103(6): 808-813, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31602500

RESUMO

Certain microbes can biotransform antibiotics. Little is known about these microbes or the biotransformation processes. The objective of this study was to determine the effects of background nutrient conditions on a sulfonamide degrading culture and on its biotransformation of sulfadiazine (SDZ) with respect to transformation kinetics and transformation products. The mixed culture capable of degrading SDZ consisted primarily of three genera, Brevibacterium, Castellaniella and Leucobacter. The maximum biotransformation rate was 4.55 mg L-1 d-1 in the absence of background nutrients. Among the three background nutrient conditions tested, diluted R2A medium lead to the highest maximum SDZ biotransformation rates, followed by humic acid and glucose. 2-aminopyrimidine was the major SDZ biotransformation product under the background nutrient conditions tested, while another previously reported biotransformation product, sulfanilic acid, was further degraded by the mixed culture. The findings from this study can help improve our estimation of the fate of antibiotics in the environment.


Assuntos
Antibacterianos/metabolismo , Meios de Cultura/química , Microbiologia do Solo , Poluentes do Solo/metabolismo , Sulfadiazina/metabolismo , Actinobacteria/metabolismo , Alcaligenaceae/metabolismo , Biodegradação Ambiental , Biotransformação , Brevibacterium/metabolismo , Glucose/química , Substâncias Húmicas/análise , Cinética , Pirimidinas/química
3.
Ecotoxicol Environ Saf ; 184: 109656, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31526920

RESUMO

The existing form of ionizable organic contaminants (IOCs) could affect their adsorption characteristics to soil and biochar. In this study, 2 IOCs, namely, sulfadiazine and imazalil, were selected to study their adsorption by rice straw-derived biochar-amended soils, as well as the effect of pH and gallic acid on their adsorption. The results showed that the soil adsorption isotherms of the two ionizable organic contaminants could be fitted well by a linear equation and the Freundlich equation, and r2 was more than 0.80. The adsorption coefficient (Kd) in the three kinds of soil ranged from 0.262 to 4.07 L kg-1 for sulfadiazine and from 3.11 to 96.5 L kg-1 for imazalil. After the addition of biochar, the adsorption of sulfadiazine and imazalil in the soil increased. The adsorption of sulfadiazine by biochar gradually decreased with the increase in pH; the adsorption of imazalil increased when the pH increased from 2 to 5 and then gradually decreased with increasing pH. Gallic acid enhanced the adsorption of the two IOCs to pure soil and biochar-amended soil.


Assuntos
Carvão Vegetal/química , Ácido Gálico/química , Oryza/química , Poluentes do Solo/análise , Solo/química , Adsorção , Concentração de Íons de Hidrogênio , Imidazóis/análise , Sulfadiazina/análise
4.
Bioresour Technol ; 294: 122178, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31563116

RESUMO

In this study, the feasibility of Fe0 addition for driving sulfadiazine (SDZ) removal during anaerobic digestion of swine manure (SM) was tested. Compared with the Fe0-free digesters spiked with 200 mg/L SDZ (RSDZ), treatments with 5.0 g/L Fe0 (RSDZ/Fe0) significantly accelerated and optimized the acidification process by enriching Clostridia and Bacteroidia (key members responsible for VFAs/H2 production), providing more readily available substrates for methanogenesis. Furthermore, Fe0 increased the overall abundance of hydrogenotrophic methanogens, specifically toxicant resistant Methanoculleus and Methanosphaera spp. were selectively enriched, helping achieve a 36.9% higher CH4 yield and a 26.4% greater total solids removal efficiency. A positive correlation between the solid content and the SDZ concentration adsorbed in SM was observed. The addition of Fe0 increased the distribution of SDZ in liquid and facilitated its removal through the enhanced biodegradation and physicochemical processes. Overall, the total SDZ removal efficiency was improved by 86.8% with Fe0.


Assuntos
Esterco , Sulfadiazina , Anaerobiose , Animais , Ácidos Graxos Voláteis , Ferro , Metano , Suínos
5.
Environ Pollut ; 255(Pt 1): 113160, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31521996

RESUMO

The intensive use of antibiotics results in the continuous release of antibiotics into wastewater treatment systems, leading to the spread of antibiotic resistance. Nitrifying system is reported to be capable of degrading antibiotics, yet few studies have systematically investigated the inherent correlation among ammonium oxidation rate, antibiotic degradation and genetic expression of nitrifying bacteria along the process. This study selected a widely used sulfonamide antibiotic, sulfadiazine (SDZ), to investigate its biodegradation potential by an enriched nitrifying culture and the response of nitrifying bacteria against antibiotic exposure. Our results demonstrated that SDZ degradation was mainly contributed by cometabolism of ammonia-oxidizing bacteria (AOB), rather than biomass adsorption. The quantitative reverse transcription PCR (RT-qPCR) analysis revealed that the expression level of amoA gene was down-regulated due to the SDZ exposure. In addition, the degradation products of SDZ did not exhibit inhibitory effect on Escherichia coli K12, indicating the biotoxicity of SDZ could be mitigated after biodegradation. The findings offer insights regarding the biodegradation process of sulfonamide antibiotics via cometabolism by AOB.


Assuntos
Antibacterianos/metabolismo , Biodegradação Ambiental , Escherichia coli K12/metabolismo , Nitrificação/fisiologia , Sulfadiazina/metabolismo , Purificação da Água/métodos , Amônia/análise , Compostos de Amônio/análise , Oxirredução , Esgotos/microbiologia , Águas Residuárias/química
6.
Environ Sci Pollut Res Int ; 26(32): 33363-33372, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31522397

RESUMO

Most sulfonamides, widely used around the world, are excreted via feces and urine along with their metabolites in humans and animals. Therefore, understanding the potential removal pathway of sulfonamides and their metabolites in wastewater treatment systems is of importance. The occurrence and fate of four sulfonamides and their acetyl metabolites in wastewater and sludge in a biological aerated filter in Xiamen city were evaluated. Six of the target compounds were detected in wastewater, but only parent compounds were detected in sludge. The highest concentration in wastewater was acetyl-sulfamethoxazole (Ac-SMZ) with a concentration of 75.2 ng/L. Removal efficiency and mass load in wastewater treatment systems were calculated. In terms of the overall removal efficiency, they ranged from 24.4 to 100%. The removal efficiencies of sulfamerazine (SM1), sulfamethazine (SM2), and sulfadiazine (SD) were up to 100% while N-acetyl sulfamerazine (Ac-SM1) showed the lowest removal efficiency. Biodegradation was the dominant remove pathway according to the mass balance analysis while SD and SM2 were sludge adsorption. The results can provide an insight into the fate of target sulfonamides in BAF systems and provide data to assess their potential ecological risks.


Assuntos
Sulfonamidas/análise , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/análise , Adsorção , Animais , Biodegradação Ambiental , China , Humanos , Esgotos/análise , Sulfadiazina , Sulfametazina , Águas Residuárias/análise
7.
Chemosphere ; 234: 978-986, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31519107

RESUMO

Batch-type experiments were used to study adsorption-desorption of three sulfonamides: sulfadiazine (SDZ) sulfachloropyridazine (SCP), and sulfamethazine (SMT), in five crop soils, whereas laboratory soil column experiments were employed to obtain data on transport processes. Adsorption results were satisfactorily adjusted to Linear and Feundlich equations, with R2 values above 0.95. Adsorption followed the sequence SDZ < SMT < SCP, showing higher values for soils with higher levels of organic carbon (OC) content. Conversely, desorption was higher in soils with less OC, and lower in soils with higher OC contents. The temporal moment analysis method gave values for the transport parameters τ and R which were significantly correlated with soil parameters related to organic matter, specifically OC and N concentrations. The higher retention of the three sulfonamides in soils with high organic matter content is a relevant fact, with value when programming management practices in agricultural soils, and specifically in relation to the spreading of animal manures, slurries, or waste containing these emerging pollutants.


Assuntos
Agricultura , Poluentes do Solo/química , Sulfacloropiridazina/química , Sulfadiazina/química , Sulfametazina/química , Adsorção , Animais , Monitoramento Ambiental , Esterco , Solo , Poluentes do Solo/análise , Sulfacloropiridazina/análise , Sulfadiazina/análise , Sulfametazina/análise , Sulfanilamida , Sulfonamidas
8.
Chemosphere ; 237: 124418, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31369901

RESUMO

Antibiotic contaminants have become a severe environmental problem in recent years and finding effective ways to deal with this issue is of great importance. In this study, Phanerochaete chrysosporium was used to degrade sulfadiazine (SDZ), which is frequently detected in the culture medium of isolates from soil and surface water systems. The results demonstrate that 10 mg L-1 SDZ can be completely degraded by P. chrysosporium under conditions of pH 5.7 and 30 °C within 6 days. The Q-Exactive-MS/MS analysis identified and confirmed several different SDZ degradation intermediates, and four proposed degradation pathways of SDZ were deduced. Moreover, enzyme activity tests revealed that manganese peroxidase and ligninolytic peroxidase played important roles in SDZ degradation. Moreover, a transcriptome analysis method was performed to explore the mechanism and pathways of SDZ degradation by P. chrysosporium in greater detail. The results of GO and KEGG analysis strongly suggest that the metabolism pathway is significantly activated and plays an important role in antibiotic degradation. Further, this is the first study to identify SDZ degradation intermediates and two main intermediates were found to be involved in possible SDZ degradation pathways. This study is also the first report results from RNA sequencing to evaluate genome-wide changes of P. chrysosporium to further explore SDZ degradation mechanism.


Assuntos
Phanerochaete/genética , Phanerochaete/metabolismo , Sulfadiazina/metabolismo , Antibacterianos/metabolismo , Biodegradação Ambiental , Poluentes Ambientais/metabolismo , Recuperação e Remediação Ambiental/métodos , Proteínas Fúngicas/metabolismo , Perfilação da Expressão Gênica , Concentração de Íons de Hidrogênio , Peroxidases/metabolismo , Espectrometria de Massas em Tandem , Fatores de Tempo
9.
Mar Pollut Bull ; 146: 591-597, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31426197

RESUMO

A molecularly imprinted polymer (MIP) anchored on the surface of CdTe quantum dots (QDs) was fabricated and used as a fluorescent probe for sulfadiazine (SDZ) detection in seawater. CdTe QDs was used as photoluminescent material, SDZ as the template, 3-aminopropyltriethoxysilane (APTES) as the functional monomer and tetraethyl orthosilicate (TEOS) as the cross-linking agent. Characterizations of MIP-QDs were analyzed by Fourier transform infrared (FT-IR), Transmission electron microscopy (TEM) and Scanning electron microscope (SEM). The conditions were optimized for the detection of MIP-QDs to SDZ. The mechanism of fluorescence quenching was studied by UV-Vis absorption spectroscopy and fluorescence spectroscopy. Under optimal conditions, the fluorescence intensity of MIP-QDs decreased linearly between 4- and 20 µM SDZ with a good correlation coefficient of 0.995. The limit of detection is 0.67 µM and the recovery is between 91.8 and 109.4% with RSD lower than 3.9%. These results indicated that MIP-QDs for SDZ detection in seawater was developed successfully.


Assuntos
Compostos de Cádmio/química , Pontos Quânticos/química , Água do Mar/análise , Sulfadiazina/análise , Telúrio/química , Poluentes Químicos da Água/análise , Antibacterianos/análise , Corantes Fluorescentes/química , Limite de Detecção , Microscopia Eletrônica de Transmissão , Impressão Molecular/métodos , Polímeros/síntese química , Polímeros/química , Propilaminas/química , Silanos/química , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier
10.
Sci Total Environ ; 691: 1072-1081, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31466189

RESUMO

Sulfonamides and their corresponding antibiotic resistance genes (ARGs) are widespread in the environment, which leads to a major threat to global health crisis. Biodegradation plays a major role in sulfonamides removal in soil ecosystem, but the degradation dynamics and the associated functional bacteria in situ remain unclear. In this study, aerobic degradation of sulfadiazine (SDZ) at two dosages (1 and 10 mg/kg) was explored for up to 70 days in two different agricultural soils. The removal of SDZ in all treatments followed first-order multi-compartment model with half-life times of 0.96-2.57 days, and DT50 prolonged with the increase of initial dosage. A total of seven bacterial genera, namely Gaiella, Clostrium_sensu_stricto_1, Tumebacillus, Roseiflexus, Variocorax, Nocardioide and Bacillus, were proposed as the potential SDZ-degraders. sadA gene was for the first time detected in soil samples, but other functional genes might also participate in SDZ degradation. The enrichment of sulfonamide resistance genes was found after 70 days' incubation, which might result in the spread of ARGs in soil. This study can add some new insights towards SDZ degradation in soil ecosystem and provide a potential resource for the bioremediation of SDZ-contaminated soil.


Assuntos
Biodegradação Ambiental , Resistência Microbiana a Medicamentos/genética , Microbiota , Microbiologia do Solo , Poluentes do Solo/análise , Sulfadiazina/análise , Solo , Poluentes do Solo/metabolismo , Sulfadiazina/metabolismo
11.
Molecules ; 24(17)2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31438527

RESUMO

Recent findings on the biological activity of thiazolidin-4-ones and taking into account the lack of effective drugs used in the treatment of toxoplasmosis, their numerous side effects, as well as the problem of drug resistance of parasites prompted us to look for new agents. We designed and synthesized a series of new thiazolidin-4-one derivatives through a two-step reaction between 4-substituted thiosemicarbazides with hydroxybenzaldehydes followed by the treatment with ethyl bromoacetate; maleic anhydride and dimethyl acetylenedicarboxylate afforded target compounds. The thiazolidin-4-one derivatives were used to assess the inhibition of Toxoplasma gondii growth in vitro. All active thiazolidine-4-one derivatives (12 compounds) inhibited T. gondii proliferation in vitro much better than used references drugs both sulfadiazine as well as the synergistic effect of sulfadiazine + trimethoprim (weight ratio 5:1). Most active among them derivatives 94 and 95 showed inhibition of proliferation at about 392-fold better than sulfadiazine and 18-fold better than sulfadiazine with trimethoprim. All active compounds (82-88 and 91-95) against T. gondii represent values from 1.75 to 15.86 (CC30/IC50) lower than no cytotoxic value (CC30).


Assuntos
Antiprotozoários/uso terapêutico , Sulfadiazina/uso terapêutico , Toxoplasma/efeitos dos fármacos , Trimetoprima/uso terapêutico , Animais , Antiprotozoários/química , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Combinação de Medicamentos , Humanos , Espectroscopia de Ressonância Magnética , Camundongos , Relação Estrutura-Atividade , Sulfadiazina/química , Tiossemicarbazonas/uso terapêutico , Toxoplasmose
12.
Int J Radiat Biol ; 95(11): 1547-1551, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31290704

RESUMO

Purpose: This study evaluated if Toxoplasma gondii infection and the drug-associated infection modifies the brain radiopharmaceutical Ethylene Cystine Diethylester Dihydrochloride (99mTc-ECD) biodistribution in mice.Materials and methods: A total of 18 mice were divided into 3 groups. Control group (C) received distilled water and 99mTc-ECD; Infected group (I) received T. gondii strain and 99mTc-ECD; Infected and Treated group (IT), in addition to infection, received association of Pyrimethamine and Sulfadiazine and 99mTc-ECD. The T. gondii strain used in this study was TgCkRrRN3. Forty minutes after administration of the 99mTc-ECD, all animals were euthanized, and blood and brain samples were isolated. The counting of the radioactivity percentage per gram of tissue (%ATI/g) was calculated, and statistical analysis was performed by t-test, with a level of significance of p < .05.Results: There was a significant increase in %ATI/g between groups C and I on brain (0.35 ± 0.02 and 0.45 ± 0.04; p = .041) and on blood (0.80 ± 0.09 and 1.14 ± 0.31; p = .049). A significant decrease in %ATI/g occurred between groups C and IT on blood (0.80 ± 0.09 and 0.54 ± 0.08; p = .001) and on brain (0.35 ± 0.02 and 0.22 ± 0.04; p = .049).Conclusions: Combined therapy of anti-Toxoplasma drugs in infected mice reduced the uptake of 99mTc-ECD, probably due to its binding to plasma proteins.


Assuntos
Encéfalo/parasitologia , Encéfalo/efeitos da radiação , Cisteína/análogos & derivados , Compostos de Organotecnécio/farmacocinética , Compostos Radiofarmacêuticos/farmacocinética , Toxoplasma , Toxoplasmose/tratamento farmacológico , Toxoplasmose/parasitologia , Animais , Antiprotozoários/uso terapêutico , Encéfalo/metabolismo , Cisteína/farmacocinética , Masculino , Camundongos , Pirimetamina/uso terapêutico , Sulfadiazina/uso terapêutico , Distribuição Tecidual
13.
Water Res ; 160: 209-216, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31152946

RESUMO

Although photochemical transformation is a major degradation pathway for antibiotics in surface freshwaters, the photodegradation of antibiotics from freshwaters downstream into seawater is largely unknown. Herein, sulfadiazine was adopted as a representative antibiotic to probe the alteration of photolytic kinetics along freshwater to seawater sampled from Qinzhou Bay, China. The results showed that the photodegradation rate constants of sulfadiazine significantly increased in estuarine waters along freshwaters to seawaters. Experiments in synthetic water samples with isolated local dissolved organic matter (IL-DOM) indicated that the increased photodegradation of sulfadiazine is attributed to the integrative effect of both IL-DOM and halide ions. Radical quenching experiments with tert-butanol (quenching of ·OH) and isopropanol (quenching of both ·OH and reactive halogen species, RHS) demonstrated that RHS are largely responsible for the halide-specific enhancement in the photodegradation of sulfadiazine, rather than other reactive species, such as triplet-excited IL-DOM and ·OH. However, triplet-excited IL-DOM was involved in the production of RHS by the oxidation of halide ions by the triplet-excited states. Experiments conducted with DOM analogues verified DOM-sensitized RHS formation, and the degradation induced by RHS is positively correlated with the triplet-excited reduction potentials of DOM analogues. These findings are helpful in deeply understanding the transformation of antibiotics, and demonstrate the importance of RHS-induced degradation in antibiotics fate models in estuarine water systems.


Assuntos
Sulfadiazina , Poluentes Químicos da Água , China , Halogênios , Fotólise
14.
Environ Sci Pollut Res Int ; 26(22): 23162-23172, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31187381

RESUMO

In aquatic environment, the existence of antibiotics including sulfadiazine (SDZ) has gain a huge attention. It is suggested that hydrous metal oxides have large potential to remove contaminants in water. The SDZ removal capability by ferric and manganese binary oxides (FMBO) was investigated, and the SDZ removal performance was compared with the ferric hydroxide (HFO) and manganese dioxide (HMO). Our results showed that SDZ removal was highly pH-dependent, but pH has less effect on uptake of SDZ on FMBO than that of the other two adsorbents. The surface acidity constant of FMBO was first calculated to be 6.31 and 8.48, respectively. The uptake process was successfully fitted for according to surface complex formation models (SCFM) and the results of modern surface analytical methods, such as FTIR and XPS, were also consistent with the surface complex uptake mechanism. The uptake of SDZ by FMBO ascribed to specific chemical interaction between the aniline group of SDZ and the hydroxyl groups from FMBO.


Assuntos
Compostos Férricos/química , Compostos de Manganês/química , Óxidos/química , Purificação da Água/métodos , Adsorção , Sulfadiazina , Água
15.
J Vet Emerg Crit Care (San Antonio) ; 29(4): 444-449, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31240801

RESUMO

OBJECTIVE: To describe the clinical presentation, diagnostic investigation, and medical management of a dog on immunosuppressive therapy that developed a severe soft tissue infection attributed to Aeromonas hydrophila/caviae. CASE SUMMARY: A 5-year-old female neutered Border Collie dog was presented for investigation of a rapidly growing skin lesion. The dog had been diagnosed with immune-mediated thrombocytopenia and was receiving immunosuppressive therapy for 5 weeks. Physical examination at initial presentation revealed no abnormalities except a 6 cm raised, erythematous, firm, and painful swelling on the ventral abdomen. Within 12 hours of admission, the lesion had expanded to cover much of the ventrum and some areas had begun to slough. The patient had also become obtunded and exhibited pyrexia, tachypnea, tachycardia as well as extreme pain around the lesion. The dog's clinical signs and hematology results were consistent with sepsis. Histopathology showed severe acute suppurative cellulitis and panniculitis and a heavy growth of A. hydrophila/caviae was obtained on tissue culture. The infection was treated with trimethoprim sulphadiazine, based on culture and susceptibility results. UNIQUE INFORMATION PROVIDED: This is the first reported case of severe panniculitis and cellulitis caused by Aeromonas spp. in a dog. Aeromonas spp. should be considered a differential diagnosis for cases of severe soft tissue infection, especially in immune-compromised animals or those with a history of aquatic exposure.


Assuntos
Aeromonas/isolamento & purificação , Celulite (Flegmão)/veterinária , Doenças do Cão/microbiologia , Infecções por Bactérias Gram-Negativas/veterinária , Sepse/veterinária , Trombocitopenia/tratamento farmacológico , Animais , Antibacterianos/uso terapêutico , Celulite (Flegmão)/microbiologia , Cães , Combinação de Medicamentos , Feminino , Infecções por Bactérias Gram-Negativas/microbiologia , Hospedeiro Imunocomprometido , Imunossupressores/efeitos adversos , Imunossupressores/uso terapêutico , Sepse/microbiologia , Sulfadiazina/uso terapêutico , Trimetoprima/uso terapêutico
16.
Bull Environ Contam Toxicol ; 103(2): 342-347, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31222425

RESUMO

The antibiotic sulfadiazine (SDZ) is a challenging threat to the health of aquatic organisms, as it frequently occurs in aquatic ecosystems. Tolerance mechanisms and accumulation of SDZ in a floating macrophyte (Eichhornia crassipes) under hydroponic conditions were investigated in this study to provide more insight into the SDZ removal process. Results show that the presence of 1 mg L-1 SDZ decreased the quickest and ranged from 669.45 to 165.34 µg L-1 from days 5 to 25. Exposing E. crassipes to SDZ ( < 1 mg L-1) maintained stable leaf photosynthetic efficiency. The overall increase in superoxide dismutase and peroxidase activities with SDZ treatments indicated that leaves were resistant. SDZ was absorbed by E. crassipes, following the sequence of root > aerial parts under all treatments. These findings suggest that E. crassipes has the ability to phytoremediation SDZ contaminated water.


Assuntos
Antibacterianos/análise , Organismos Aquáticos/crescimento & desenvolvimento , Eichhornia/crescimento & desenvolvimento , Sulfadiazina/análise , Poluentes Químicos da Água/análise , Antibacterianos/metabolismo , Organismos Aquáticos/metabolismo , Biodegradação Ambiental , Eichhornia/metabolismo , Hidroponia , Modelos Teóricos , Sulfadiazina/metabolismo , Poluentes Químicos da Água/metabolismo
17.
Pharm Res ; 36(8): 122, 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31218556

RESUMO

PURPOSE: A non-propellant based foam (NPF) system was developed incorporating the antibiotics, pectin capped green nano-silver and sulfadiazine (SD) for the topical treatment of burn wounds as a convenient alternative to the existing therapies. METHODS: NPF were prepared using various surfactants and oils forming a nanoemulsion. Anti-microbial studies by resazurin microtitre assay, ex vivo diffusion, in vivo skin permeation and deposition studies, and acute irritation studies were carried out. NPF was applied onto secondary thermal wounds manifested on mice models followed by macroscopic and histological examinations. RESULTS: NPF had an average globule size of <75 nm. The viscosity was ~10 cP indicating the feasibility of expulsion from the container upon actuation. With no skin irritation, the foams showed a higher skin deposition of SD. A high contraction and an evident regeneration of the skin tissue upon treatment with NPF indicated a good recovery from the thermal injury was apparent from the histology studies. CONCLUSION: NPF represents an alternative topical formulation that can be employed as a safe and effective treatment modality for superficial second degree (partial thickness) burn wounds. With a minimal requirement of mechanical force, the no-touch application of NPF makes it suitable for sensitive and irritant skin surfaces.


Assuntos
Antibacterianos/farmacologia , Queimaduras/tratamento farmacológico , Nanopartículas Metálicas/química , Prata/química , Sulfadiazina/farmacologia , Cicatrização/efeitos dos fármacos , Administração Tópica , Animais , Antibacterianos/administração & dosagem , Queimaduras/patologia , Queimaduras/fisiopatologia , Composição de Medicamentos/métodos , Quimioterapia Combinada , Emulsões , Escherichia coli/efeitos dos fármacos , Química Verde , Humanos , Masculino , Camundongos , Óleos/química , Tamanho da Partícula , Permeabilidade , Pele/efeitos dos fármacos , Pele/patologia , Pele/fisiopatologia , Staphylococcus aureus/efeitos dos fármacos , Sulfadiazina/administração & dosagem , Tensoativos/química
18.
Exp Parasitol ; 202: 7-14, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31077733

RESUMO

Toxoplasmosis in South America presents great health impacts and is a topic of research interest not only because of the severity of native cases but also due to the predominant atypical genotypes of the parasite circulating in this continent. Typically, symptomatic toxoplasmosis is treated with a combination of sulfadiazine (SDZ) and pyrimethamine (PYR). However, some clinical cases present treatment failures due to an inability of the drugs to control the infection or their significant adverse effects, which can lead to treatment interruption. Although resistance/susceptibility to the aforementioned drugs has been well described for clonal strains of Toxoplasma gondii spread to the Northern Hemisphere, less is known about the South American atypical strains. In this study, the effectiveness of SDZ and PYR for the treatment of mice during acute infection with different atypical T. gondii strains was evaluated. Swiss mice were infected with seven T. gondii strains obtained from newborn patients with congenital toxoplasmosis in Brazil. The infected mice were treated with 10-640 mg/kg per day of SDZ, 3-200 mg/kg per day of PYR, or a combination of both drugs with a lower dosage. The mice were evaluated for parameters including mortality, anti-T. gondii IgG production by ELISA and the presence of brain cysts. In addition, the presence of polymorphisms in the dhps gene was verified by gene sequencing. A descriptive analysis was used to assess the association between susceptibility to SDZ and/or PYR and the genotype. The TgCTBr4 and TgCTBr17 strains (genotype 108) presented lower susceptibility to SDZ or PYR treatment. The TgCTBr1 and TgCTBr25 strains (genotype 206) presented similar susceptibility to PYR but not SDZ treatment. The TgCTBr9 strain (genotype 11) was the only strain with high susceptibility to treatment with both drugs. The TgCTBr13 strain (genotype 208) was not susceptible to treatment with the lower PYR or SDZ doses. The TgCTBR23 strain (genotype 41) was more susceptible to PYR than to SDZ treatment. However, the association of low SDZ and PYR doses showed good efficacy for the treatment of experimental toxoplasmosis with T. gondii atypical strains obtained from newborns in Brazil. A new mutation in the T. gondii dhps gene (I347M) was identified that might be associated with the SDZ low sensitivity profile observed for the TgCTBr4 and TgCTBr17 isolates.


Assuntos
Antiprotozoários/uso terapêutico , Pirimetamina/uso terapêutico , Sulfadiazina/uso terapêutico , Toxoplasmose Animal/tratamento farmacológico , Toxoplasmose Congênita/parasitologia , Álcool Desidrogenase/genética , Animais , Antiprotozoários/farmacologia , Feminino , Genótipo , Humanos , Recém-Nascido , Camundongos , Pirimetamina/farmacologia , Sulfadiazina/farmacologia , Toxoplasma/classificação , Toxoplasma/efeitos dos fármacos , Toxoplasma/genética , Toxoplasma/patogenicidade , Toxoplasmose Animal/parasitologia , Toxoplasmose Congênita/tratamento farmacológico , Virulência
19.
Environ Pollut ; 248: 1079-1087, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31091640

RESUMO

This work present aims to evaluate the effect of a conventional wastewater treatment process on the number of nanoparticles, and the role of nanoparticles as a carrier of antibiotics. A set of methods based on asymmetrical flow field flow fractionation coupled with multi-angle light scattering to separate and quantify nanoparticles in real wastewater was established. The characterization of nanoparticles was conducted by transmission electron microscopy, energy dispersive spectrometer, UV-visible spectrophotometer and three-dimensional excitation-emission matrix fluorescence spectroscopy. The adsorption of different sizes of nanoparticles separated from the real wastewater for four targeted antibiotics (sulfadiazine, ofloxacin, tylosin and tetracycline) was studied. The results show that the number of nanoparticles were increased in the wastewater treatment process and the size range between 60 and 80 nm was predominant in wastewater samples. The nanoparticles were mainly composed of O, Si, Al and Ca elements and organic components were in the size range of 0-10 nm. Targeted antibiotics were dominantly adsorbed onto nanoparticles with 60-80 nm size range at each stage. The concentrations of tetracycline adsorbed on nanoparticles were surprisingly increased in the end of the treatment process, while ofloxacin and tylosin had the completely opposite phenomenon to tetracycline. The pH and ionic strength definitely affected the aggregation of nanoparticles and interaction with the antibiotics. It is of great significance to give insights into nanoparticle-antibiotic assemblages for the effective treatment and avoiding the water risks due to nanoparticles' ubiquitous and their risks of carrying antibiotics.


Assuntos
Antibacterianos/análise , Antibacterianos/química , Nanopartículas/química , Ofloxacino/análise , Sulfadiazina/análise , Tetraciclina/análise , Tilosina/análise , Águas Residuárias/química , Purificação da Água/métodos , Adsorção , Fracionamento por Campo e Fluxo , Microscopia Eletrônica de Transmissão , Ofloxacino/química , Concentração Osmolar , Espectrometria por Raios X , Análise Espectral , Sulfadiazina/química , Tetraciclina/química , Tilosina/química , Água/química
20.
Ecotoxicol Environ Saf ; 178: 146-158, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31002969

RESUMO

Animal manure or bio-solids used as fertilizers are the main routes of antibiotic exposure in the agricultural land, which can have immense detrimental effects on plants. Sulfadiazine (SDZ), belonging to the class of sulfonamides, is one of the most detected antibiotics in the agricultural soil. In this study, the effect of SDZ on the growth, changes in antioxidant metabolite content and enzyme activities related to oxidative stress were analysed. Moreover, the proteome alterations in Arabidopsis thaliana roots in response to SDZ was examined by means of a combined iTRAQ-LC-MS/MS quantitative proteomics approach. A dose-dependent decrease in leaf biomass and root length was evidenced in response to SDZ. Increased malondialdehyde content at higher concentration (2 µM) of SDZ indicated increased lipid peroxidation and suggest the induction of oxidative stress. Glutathione levels were significantly higher compared to control, whereas there was no increase in ascorbate content or the enzyme activities of glutathione metabolism, even at higher concentrations. In total, 48 differentially abundant proteins related to stress/stimuli response followed by transcription and translation, metabolism, transport and other functions were identified. Several proteins related to oxidative, dehydration, salinity and heavy metal stresses were represented. Upregulation of peroxidases was validated with total peroxidase activity. Pathway analysis provided an indication of increased phenylpropanoid biosynthesis. Probable molecular mechanisms altered in response to SDZ are highlighted.


Assuntos
Antibacterianos/toxicidade , Arabidopsis/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Proteoma/metabolismo , Poluentes do Solo/toxicidade , Sulfadiazina/toxicidade , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Fertilizantes/análise , Esterco/análise , Proteômica/métodos , Solo/química
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