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1.
Sci Total Environ ; 754: 142180, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32920410

RESUMO

Residues from hydrogen sulfide (H2S) removal in biogas filters contain sulfur (S) in various forms (sulfate, sulfide, elemental S) that, if properly stored, is potentially valuable as crop fertilizer. We investigated 1) the turnover of the S compounds from filter materials during storage in untreated and digested cattle manure (CM), and 2) the S fertilizer replacement value (SFRV) of the filter materials applied in pure form or mixed manure with and without storage. The S filter materials from four H2S removal processes (biological and physical-chemical) containing mostly sulfate and/or elemental S were added to untreated CM or digested CM and stored at 10 °C for six months. Afterwards, a pot experiment was established to assess the S availability in an oil-seed rape (Brassica napus) crop. Microbial reduction of sulfate into sulfide took place rapidly after 69 days storage of untreated CM. A lower reduction rate was observed in digested CM mixtures. After six months, 68% and 32% of the initial sulfate content were still present in mixtures containing the S filter materials from biological desulfurization with digested CM and untreated CM, respectively. Sulfate reduction was inhibited for 120 days when digested CM was mixed with S saturated solution from an ash filter, probably due to high pH (≥8.2) and redox potential (>-100 mV) levels. Oppositely, elemental S was immediately and simultaneously both reduced and oxidized. Relatively low losses of total S were observed during the present storage conditions. Despite S turnover, the SFRV of CM and digested CM significantly increased from 15-19% (of total S applied) to 56-90% when S filter materials were added. The storage of S filter materials in digested manure reduced the risk of sulfide production and potential S volatilization. The S filter materials were a valuable source of plant-available S.


Assuntos
Sulfeto de Hidrogênio , Esterco , Animais , Biocombustíveis , Reatores Biológicos , Bovinos , Sulfeto de Hidrogênio/análise , Sulfatos , Enxofre
2.
Water Sci Technol ; 82(11): 2271-2281, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33339783

RESUMO

Field work was performed to investigate the release of hydrogen sulphide (H2S) and its transport in the sewer trunk with drops in the Bonnie Doon area in Edmonton, Alberta, Canada, in order to develop a proper odor control strategy. The liquid sulfide concentration in the upstream trunk was low (less than 1.0 mg/L), and no H2S gas was detected in the head space under this low concentration. However, high H2S gas concentration was detected in the middle reach of the trunk due to the stripping effect of the three drops (2.7 m, 5.2 m and 2.0 m) along the trunk. The released H2S at drops was then transported in the sewer system and emitted at various locations and caused odor concerns. These drops played an important role in H2S release, and the overall H2S mass transfer coefficient at drops was much higher than that in normal gravity sewers. The overall oxygen and H2S mass transfer coefficient (KLa) was estimated to be around 200 h-1 and 300 h-1 at the first two drops, respectively. Field sampling of biofilm indicates that Desulfomicrobium was identified as the sulfate-reducing bacteria (SRB) responsible for sulfide generation in sewer wall biofilm and Thiobacillus was the only predominant member in manhole wall biofilm contributing to sewer manhole corrosion.


Assuntos
Sulfeto de Hidrogênio , Esgotos , Alberta , Corrosão , Sulfeto de Hidrogênio/análise , Sulfetos
3.
J Chromatogr A ; 1625: 461243, 2020 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-32709315

RESUMO

The long identified toxic gas, hydrogen sulfide (H2S), which has also been confirmed as the third gaseous signaling molecule following NO and CO, plays important roles in various physiological and pathological process. The current most established quantification method for H2S is HPLC method coupled with fluorescence detection after derivatization with a costly fluorescent reagent, Monobromobimane (MBB). However, The MBB method is characterized by strict reaction condition, long reaction time, tedious operation, and inconsistent reported results. In this study, based on the thiolysis reaction of 7-nitro-2, 1, 3-benzoxadiazole (NBD) ether, the commonly used chromatographic modifier 4-chloro-7-nitro-2,1,3- benzoxadiazole (NBDCl) and four probes (NBDOMe, NBDOEt, NBDOTFE and NBDOCMR) synthesized from NBDCl were tested as alternatives for fast quantification of H2S by LC-MS/MS. The reaction product between NBD ethers/NBDCl and H2S showed special pink color visible to the naked eye and was easy to synthesize and separate in lab; it also showed good retention on common chromatographic columns and high instrument response; therefore it is a good determinand. After establishment of LC-MS/MS methods for all the related compounds, the reaction conditions were optimized for all the probes with H2S. Then the stability, selectivity, reaction rate, sensitivity and quantitative linear relationship between the reaction product and H2S concentration were studied for each probe. Finally, NBDOEt was selected for LC-MS/MS detection of H2S. In comparision with the MBB method, the established NBDOEt method showed matched sensitivity and linearity, better selectivity, and higher repeatability; and had the advantages of easy operation, simple reaction condition, and cheap raw materials. The method was successfully validated and applied to determination of Na2S content in Na2S∙9H2O bulk drug and injection. In conclusion, NBDOEt is a promising option for quantification of H2S in abiotic matrix.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Éter/química , Sulfeto de Hidrogênio/análise , Espectrometria de Massas em Tandem/métodos , Compostos Bicíclicos com Pontes/química , Sulfeto de Hidrogênio/química , Concentração de Íons de Hidrogênio , Oxidiazóis/química , Reprodutibilidade dos Testes
4.
Environ Sci Pollut Res Int ; 27(30): 37684-37698, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32608005

RESUMO

Airborne emissions from concentrated animal feeding operations (CAFOs) have the potential to pose a risk to human health and the environment. Here, we present an assessment of the emission, dispersion, and health-related impact of ammonia and hydrogen sulfide emitted from a 300-head, full-scale dairy farm with an exercise yard in Beijing, China. By monitoring the referred gas emissions with a dynamic flux chamber for seven consecutive days, we examined their emission rates. An annual hourly emission time series was constructed on the basis of the measured emission rates and a release modification model. The health risk of ammonia and hydrogen sulfide emissions around the dairy farm was then determined using atmospheric dispersion modeling and exposure risk assessment. The body mass-related mean emission factors of ammonia and hydrogen sulfide were 2.13 kg a-1 AU-1 and 24.9 g a-1 AU-1, respectively (one animal unit (AU) is equivalent to 500 kg body mass). A log-normal distribution fitted well to ammonia emission rates. Contour lines of predicted hourly mean concentrations of ammonia and hydrogen sulfide were mainly driven by the meteorological conditions. The concentrations of ammonia and hydrogen sulfide at the fence line were below 10 µg m-3 and 0.04 µg m-3, respectively, and were 2-3 orders of magnitude lower than the current Chinese air quality standards for such pollutants. Moreover, the cumulative non-carcinogenic risks (HI) of ammonia and hydrogen sulfide were 4 orders of magnitudes lower than the acceptable risk levels (HI = 1). Considering a health risk criterion of 1E-4, the maximum distance from the farm fence line to meet this criterion was nearly 1000 m towards north-northeast. The encompassed area of the contour lines of the ambient concentration of ammonia is much larger than that of hydrogen sulfide. However, the contour lines of the ammonia health risk are analogous to those of hydrogen sulfide. In general, the ammonia and hydrogen sulfide emissions from the dairy farm are unlikely to cause any health risks for the population living in the neighborhood.


Assuntos
Poluentes Atmosféricos/análise , Sulfeto de Hidrogênio/análise , Amônia/análise , Animais , Pequim , China , Monitoramento Ambiental , Fazendas , Humanos
5.
PLoS One ; 15(5): e0232742, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32374788

RESUMO

Caves formed by sulfuric acid dissolution have been identified worldwide. These caves can host diverse microbial communities that are responsible for speleogenesis and speleothem formation. It is not well understood how microbial communities change in response to surface water entering caves. Illumina 16S rRNA sequencing and bioinformatic tools were used to determine the impact of surface water on the microbial community diversity and function within a spring pool found deep in the Monte Conca Cave system in Sicily, Italy. Sulfur oxidizers comprised more than 90% of the microbial community during the dry season and were replaced by potential anthropogenic contaminants such as Escherichia and Lysinibacillus species after heavy rains. One sampling date appeared to show a transition between the wet and dry seasons when potential anthropogenic contaminants (67.3%), sulfur-oxidizing bacteria (13.6%), and nitrogen-fixing bacteria (6.5%) were all present within the spring pool.


Assuntos
Cavernas/microbiologia , Microbiota/genética , Microbiologia da Água , Molhabilidade , Bacillaceae/genética , Sequência de Bases , DNA Bacteriano/genética , Secas , Escherichia/genética , Sulfeto de Hidrogênio/análise , RNA Ribossômico 16S/genética , Chuva , Estações do Ano , Sicília , Microbiologia do Solo , Sulfatos/análise
6.
Chemosphere ; 256: 127078, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32473468

RESUMO

This study investigated the feasibility of co-treating H2S and CO2 in a biological trickling filter (BTF) inoculated with hydrogenotrophic methanogens (HMs) and nitrate-reducing, sulfur-oxidizing bacteria. This was accomplished by introducing a pure culture of Thiobacillus denitrificans in a BTF that was successfully upgrading a biogas mimic (60:40 CH4:CO2) to >97% methane using an enriched HM consortium. Nitrate was fed as the electron acceptor to oxidize H2S. The results revealed that a severe competition for hydrogen's electrons occurred between carbon dioxide and nitrate. Due to this competition, N:S loading rates of 16:1 were required to achieve >98% H2S removal, a ratio which is four times greater than the theoretical N:S ratio for complete sulfur oxidation. However, such high nitrate loading rates (>50 g N-NO3- m-3 h-1) had a negative impact on the BTF's biogas upgrading performance. An electron balance illustrated the increasing diversion of H2 electrons towards nitrate reduction as nitrate loading increased. Overall, this study showed that simultaneous biogas upgrading and H2S removal in a single bioreactor is possible, but that achieving high yields for both reactions requires further research in process and culture optimization.


Assuntos
Biocombustíveis , Dióxido de Carbono/análise , Sulfeto de Hidrogênio/análise , Bactérias , Reatores Biológicos/microbiologia , Hidrogênio , Metano , Nitratos
7.
J Breath Res ; 14(3): 036005, 2020 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-32428892

RESUMO

To investigate whether academic stress changes the salivary microbiota and its relationship with salivary parameters, evaluating the effects on the production of volatile sulfur compounds (VSCs) in healthy women. Fifty-five women who were enrolled in a four-year Dentistry course were assessed for academic stress by the questionnaire Maslach Burnout Inventory-Student Survey and were then classified into 'Not Stressed' and 'Stressed' groups. Cortisol and alpha-amylase (AA) were measured as physiological stress biomarkers. Oral Chroma™ gas chromatograph was used to measure concentrations of hydrogen sulfide (H2S), methyl mercaptan and dimethyl sulfide. Salivary proteins were evaluated by western blot. Quantitative polymerase chain reaction was used to identify the salivary amounts of halitosis-associated bacteria. The 'Stressed' volunteers showed higher levels of H2S, AA, Fusobacterium nucleatum and total bacteria, compared to the 'Not Stressed' individuals (p < 0.05; Mann-Whitney test). Salivary proteins showed no differences between groups (p > 0.05; Mann-Whitney test). Academic stress was positively correlated with H2S, total bacteria and F. nucleatum counts, while F. nucleatum was positively correlated with AA. H2S showed positive correlations with AA and Solobacterium moorei (p < 0.05; Spearman correlation). Beta-defensin (BD) presented negative correlations with H2S and S. moorei (p < 0.05; Spearman correlation). Academic stress increased salivary F. nucleatum and total bacteria, as well as AA activity. The protein BD showed important correlations with bacteria and VSC. These changes appeared to be accountable for increased H2S production in the stressed women.


Assuntos
Biomarcadores/análise , Halitose/microbiologia , Boca/microbiologia , Estresse Psicológico/microbiologia , Estudantes , Compostos de Enxofre/análise , Adolescente , Bactérias/metabolismo , Testes Respiratórios , Feminino , Humanos , Sulfeto de Hidrogênio/análise , Proteínas e Peptídeos Salivares/metabolismo , Compostos Orgânicos Voláteis/análise , Adulto Jovem , alfa-Amilases/análise , beta-Defensinas/análise
8.
Food Chem ; 324: 126893, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32344336

RESUMO

An Au patch electrode Ag-SnO2/SiO2/Si MIS capacitive sensor equipped with a microcontroller was designed and developed to sense low concentration (ppb to ppm regime) of volatiles (NH3, TMA, ethanol, and H2S) generated from chicken meat spoilage at room temperature. The quality threshold or the acceptance limit for consumption of chicken meat samples stored at 4 °C, 15 °C and 25 °C using our proposed technique was found to be 105 h, 48 h, and 17 h respectively, highly correlated with TVB-N, TVC, pH and sensory evaluation analysis. When these well established standard methods (TVB-N, TVC and pH analysis) take many hours to complete the analysis involving many complicated steps, our fabricated sensor takes 55 sec to deliver sensing response reflecting the meat spoilage status. The sensor calibrated with our compact technique promises portable and inexpensive onsite rapid and accurate quality assessment of meat spoilage at room temperature.


Assuntos
Gases/análise , Ouro/química , Carne/análise , Semicondutores , Amônia/análise , Animais , Galinhas , Eletrodos , Qualidade dos Alimentos , Sulfeto de Hidrogênio/análise , Concentração de Íons de Hidrogênio , Silício/química , Dióxido de Silício/química , Prata/química , Temperatura , Compostos de Estanho/química
9.
Sci Total Environ ; 724: 137977, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32247972

RESUMO

The biogas produced in food waste anaerobic digestion (FWAD) contains H2S which can lead to corrosion, bad smell and poisoning accident. To control H2S pollution, the characteristics and mechanisms of H2S production in FWAD should be known. In this study, a lab-scale FWAD batch test was applied for 20 days under 35 °C. The production potential and average concentration of H2S were 765 ± 163 g/t (TS) and 1065 ± 267 ppm, respectively. 76% of total H2S was produced within 6 h on the first day of fermentation, acidification and gas production were key reasons for high H2S production at this time. Compared to H2S peak production time, that of methane was long (4 days) and after that of H2S. Sulfides were found to be the dominant form of sulfur (accounting for 20-70% of total sulfur) in the mixed fermentation liquor in fermentation batch. These sulfides were from protein, which could be decomposed slowly to sulfide by protein-using bacteria and methanogen at the time of methane production peak, and sulfate, which could be converted to sulfide by Sulfate reducing bacteria (SRB) during the first two days of fermentation. Protein would be the main contributor to sulfide/H2S for the continuous feeding FWAD system in long term operation, due to its presence as the main form of sulfur in food waste.


Assuntos
Sulfeto de Hidrogênio/análise , Eliminação de Resíduos , Anaerobiose , Biocombustíveis , Reatores Biológicos , Alimentos , Metano
10.
Respir Investig ; 58(3): 212-215, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32171700

RESUMO

Endobronchial volatile sulfur compounds in patients with lung abscess or lung cancer were measured using the Oral Chroma™ gas chromatograph. High levels of hydrogen sulfide and methyl mercaptan were observed in some patients with lung abscess but not in patients with lung cancer. Measuring endobronchial volatile sulfur compounds could be useful for the rapid diagnosis of lung abscess caused by obligate anaerobes.


Assuntos
Bactérias Anaeróbias , Infecções Bacterianas/diagnóstico por imagem , Infecções Bacterianas/microbiologia , Brônquios/metabolismo , Cromatografia Gasosa/métodos , Abscesso Pulmonar/diagnóstico , Abscesso Pulmonar/microbiologia , Compostos de Enxofre/análise , Biomarcadores/análise , Humanos , Sulfeto de Hidrogênio/análise , Projetos Piloto , Volatilização
11.
Chemosphere ; 248: 126010, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32028160

RESUMO

This paper firstly reported a systematic study of using alum sludge (waterworks residue) for H2S adsorption. Various trials were performed at ambient temperature in a fixed bed column to study the effects of H2S flow rate, sorbent bed depth on the alum sludge adsorption efficiency of H2S. The Breakthrough Curves were simulated by the Thomas model, Bed Depth Service Time model and Yoon-Nelson models. The mechanisms of H2S adsorption onto alum sludge was examined by different physiochemical characterizations of exhausted and raw alum sludge. Moreover, the mass transfer coefficients were determined from mathematical descriptions of breakthrough curves. The alum sludge adsorption capacity was determined to be 374.2 mg of H2S/g, slightly decreasing with the increasing flow rate and increasing with the increasing bed depth. All the three models successfully predict breakthrough curves which could be used for scaling-up purposes. The microporous structure, alkaline pH and the inherent metal species of the alum sludge promoted the formation of metal sulphate species. This study demonstrated that alum sludge could be used as cost-effective, largely available, and efficient sorbent for H2S removal.


Assuntos
Sulfeto de Hidrogênio/química , Modelos Químicos , Eliminação de Resíduos Líquidos/métodos , Adsorção , Compostos de Alúmen/química , Sulfeto de Hidrogênio/análise , Modelos Teóricos , Esgotos
12.
Chem Commun (Camb) ; 56(19): 2849-2852, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32031557

RESUMO

HClO and H2S are crucial for maintaining the homeostasis in cells and play vital roles in many physiological and pathological processes. Herein, we present a fluorescent probe that can respectively and simultaneously detect H2S and HClO in a dual-ratiometric manner with good linearity. Utilizing this probe, the imaging of intracellular H2S or/and HClO in living cells in a ratiometric manner was achieved.


Assuntos
Corantes Fluorescentes/química , Sulfeto de Hidrogênio/análise , Ácido Hipocloroso/análise , Fluorescência , Homeostase , Humanos , Células MCF-7
13.
Spectrochim Acta A Mol Biomol Spectrosc ; 229: 117974, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31927478

RESUMO

Colorimetric and fluorescent detection of cyanide and hydrogen sulfide ions has been effected using a simple organic probe in H2O:DMSO (20:80, v/v) medium. The probe exhibits a colour change from pale-yellow to red upon addition of these analytes under normal light and fluorescent change from green to red under UV lamp. Other competitive ions show no observable colour or fluorescence change. The binding constants of cyanide and hydrogen sulfide ions with the probe determined using fluorescence titration data are found to be 2.1 × 104 and 1.6 × 104 M-1, respectively. The probe fluorimetrically detects the analytes in a wide pH range (4-10). 1H and 13C NMR studies suggest that the probe senses cyanide ion through deprotonation and nucleophilic addition mechanism and hydrogen sulfide ion via deprotonation mechanism. Detection limits of cyanide and hydrogen sulfide are determined to be 0.15 and 1 µM, respectively. The practical utility of the probe has been demonstrated by same dual mode detection of cyanide in food materials like bitter almond, cassava flour and sprouting potato.


Assuntos
Colorimetria/métodos , Cianetos/análise , Sulfeto de Hidrogênio/análise , Iminas/química , Espectrometria de Fluorescência/métodos , Poluentes Químicos da Água/análise , Corantes Fluorescentes/química , Manihot/metabolismo , Prunus/metabolismo , Solanum tuberosum/metabolismo
14.
J Sci Food Agric ; 100(5): 2305-2312, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31960971

RESUMO

BACKGROUND: Aquaculture is the fastest growing food-production sector, and common carp (Cyprinus carpio) is one of the most cultivated fish species in the world. Due to its intrinsic characteristics, fish meat is highly susceptible to microbiological spoilage. Pseudomonas and Shewanella are the primary and secondary occurring microbiota during storage of fish meat, with significant contribution to spoilage with the formation of hydrolytic enzymes (lipases and proteases). RESULTS: With in vitro testing, we show that rosemary extract (Inolens4), buffered vinegar and their combination (SyneROX) exhibit antimicrobial effects against P. fragi, P. psychrophila, S. putrefaciens and S. xiaemensis at concentrations of 3.13 and 1.56 mg mL-1 . The combination was the most effective in inhibiting growth of selected bacteria in food model, and production of lipases and proteases during 9 days at 5 °C. In situ testing of antimicrobial dip treatment of carp meat determined that aerobic mesophilic, total psychrotrophic, Pseudomonas and hydrogen sulfide producer counts were reduced in all treatments, with the most prominent influence being shown by the combination and buffered vinegar. CONCLUSIONS: Our study highlights the importance of a multilevel assessment of the antimicrobial potential of biopreservatives under conditions comparable to those of the selected food. Investigation with bacteria and food model provided coherent and consistent data for the evaluation of the antimicrobial potential for carp meat. Combination of buffered vinegar (as active antimicrobial) and rosemary extract, with well-known and researched antioxidant properties but low in situ antimicrobial activity, represents a good potential for combined effect in preservation of fish meat. © 2020 Society of Chemical Industry.


Assuntos
Ácido Acético/antagonistas & inibidores , Carpas/microbiologia , Extratos Vegetais/química , Pseudomonas/efeitos dos fármacos , Rosmarinus/química , Shewanella/efeitos dos fármacos , Animais , Antibacterianos/farmacologia , Aquicultura , Microbiologia de Alimentos , Conservação de Alimentos , Sulfeto de Hidrogênio/análise , Testes de Sensibilidade Microbiana , Pseudomonas/crescimento & desenvolvimento , Alimentos Marinhos/microbiologia , Shewanella/crescimento & desenvolvimento , Paladar
15.
Molecules ; 25(3)2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31973081

RESUMO

Near-infrared (NIR) fluorescent probes are attractive tools for bioimaging applications because of their low auto-fluorescence interference, minimal damage to living samples, and deep tissue penetration. H2S is a gaseous signaling molecule that is involved in redox homeostasis and numerous biological processes in vivo. To this end, we have developed a new red shifted fluorescent probe 1 to detect physiological H2S in live cells. The probe 1 is based on a rhodamine derivative as the red shifted fluorophore and the thiolysis of 7-nitro 1,2,3-benzoxadiazole (NBD) amine as the H2S receptor. The probe 1 displays fast fluorescent enhancement at 660 nm (about 10-fold turn-ons, k2 = 29.8 M-1s-1) after reacting with H2S in buffer (pH 7.4), and the fluorescence quantum yield of the activated red shifted product can reach 0.29. The probe 1 also exhibits high selectivity and sensitivity towards H2S. Moreover, 1 is cell-membrane-permeable and mitochondria-targeting, and can be used for imaging of endogenous H2S in living cells. We believe that this red shifted fluorescent probe can be a useful tool for studies of H2S biology.


Assuntos
Corantes Fluorescentes/metabolismo , Células Endoteliais da Veia Umbilical Humana/metabolismo , Sulfeto de Hidrogênio/análise , Sobrevivência Celular , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/química , Células HeLa , Humanos , Cinética , Mitocôndrias/metabolismo , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Fatores de Tempo
16.
Nat Commun ; 11(1): 446, 2020 01 23.
Artigo em Inglês | MEDLINE | ID: mdl-31974383

RESUMO

Afterglow luminescent probes with high signal-to-background ratio show promise for in vivo imaging; however, such probes that can be selectively delivered into target sites and switch on afterglow luminescence remain limited. We optimize an organic electrochromic material and integrate it into near-infrared (NIR) photosensitizer (silicon 2,3-naphthalocyanine bis(trihexylsilyloxide) and (poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene]) containing nanoparticles, developing an H2S-activatable NIR afterglow probe (F12+-ANP). F12+-ANP displays a fast reaction rate (1563 ± 141 M-1 s-1) and large afterglow turn-on ratio (~122-fold) toward H2S, enabling high-sensitivity and -specificity measurement of H2S concentration in bloods from healthy persons, hepatic or colorectal cancer patients. We further construct a hepatic-tumor-targeting and H2S-activatable afterglow probe (F12+-ANP-Gal) for noninvasive, real-time imaging of tiny subcutaneous HepG2 tumors (<3 mm in diameter) and orthotopic liver tumors in mice. Strikingly, F12+-ANP-Gal accurately delineates tumor margins in excised hepatic cancer specimens, which may facilitate intraoperative guidance of hepatic cancer surgery.


Assuntos
Carcinoma Hepatocelular/diagnóstico por imagem , Sulfeto de Hidrogênio/análise , Neoplasias Hepáticas/diagnóstico por imagem , Substâncias Luminescentes/química , Imagem Molecular/métodos , Animais , Neoplasias Colorretais/sangue , Cistationina beta-Sintase/análise , Cistationina beta-Sintase/metabolismo , Cistationina gama-Liase/análise , Cistationina gama-Liase/metabolismo , Células Hep G2 , Humanos , Sulfeto de Hidrogênio/sangue , Sulfeto de Hidrogênio/química , Neoplasias Hepáticas/sangue , Neoplasias Hepáticas Experimentais/diagnóstico por imagem , Substâncias Luminescentes/síntese química , Camundongos Endogâmicos BALB C , Nanopartículas/química , Fármacos Fotossensibilizantes/química , Polímeros/química , Compostos de Vinila/química , Ensaios Antitumorais Modelo de Xenoenxerto
17.
Anal Chim Acta ; 1097: 238-244, 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-31910965

RESUMO

Hydrogen sulfide (H2S), cysteine (Cys) and homocysteine (Hcy) play critical roles in human pathologies and there are close interconnections between them in their generation and metabolism in living systems. To elucidate their complex interplay networks, single-molecule fluorescent probes enabling simultaneous detection of H2S and Cys/Hcy from distinct emission channels have been becoming indispensable tools. In this report, we have rationally developed a novel fluorescent probe, NC, for H2S and Cys/Hcy by integrating an amino 7-nitro-1, 2, 3-benzoxadiazole (NBD) moiety and an azide group into the coumarin platform. NC exhibited good selectivity and sensitivity for the discriminatory detection of H2S and Cys/Hcy, and its capability for imaging of intracellular H2S and Cys/Hcy was proved.


Assuntos
Cisteína/análise , Corantes Fluorescentes/química , Homocisteína/análise , Sulfeto de Hidrogênio/análise , Corantes Fluorescentes/síntese química , Células HeLa , Humanos , Estrutura Molecular , Imagem Óptica , Células Tumorais Cultivadas
18.
Spectrochim Acta A Mol Biomol Spectrosc ; 229: 118003, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31923786

RESUMO

Biological thiols including homocysteine (Hcy), cysteine (Cys), hydrogen sulfide (H2S) and glutathione (GSH) play crucial roles in various pathological and physiological processes. The development of optical probes for biothiols has been an active research area in recent years. Herein, a new turn-on fluorescence probe (HD-NBD) was designed and synthesized by fusing tetrahydro[5]helicene and 7-nitro-2,1,3-benzoxadiazole (NBD) for simultaneous discrimination of Hcy/Cys, H2S and GSH in aqueous solution. This probe is able to show unique absorbance enhancement at 548 nm for H2S and additional fluorescence enhancement at 536 nm only for Cys/Hcy, which can be used to discriminate H2S, Cys/Hcy and GSH simultaneously. In addition, HD-NBD also shows low background without any self-fluorescence, as well as high selectivity toward common biothiols. The low detection limits of this probe are about 0.15 µM for Hcy with a wide linear range (1-80 µM), 0.36 µM for Cys (linear range: 1-45 µM), 0.79 µM for H2S (linear range: 1-80 µM) and 4.44 µM for GSH (linear range: 1-60 µM). Moreover, HD-NBD can identify Hcy/Cys, H2S from GSH and other amino acids with high sensitivity and selectivity, therefore it could be used for detecting endogenous and exogenous Hcy/Cys under biological condition.


Assuntos
Neoplasias da Mama/metabolismo , Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Sulfeto de Hidrogênio/análise , Neoplasias da Mama/patologia , Dinitrobenzenos/química , Feminino , Humanos , Oxidiazóis/química , Compostos Policíclicos/química , Células Tumorais Cultivadas
19.
Spectrochim Acta A Mol Biomol Spectrosc ; 224: 117410, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31352139

RESUMO

Hydrogen polysulfides (H2Sn, n>1) plays crucial roles in many biological processes, while it remains a challenge for rapid and selective detection of H2Sn. We designed and synthesized a turn-on fluorescent probe (JCCF) for detecting H2Sn based on a new julolidine-coumarinocoumarin scaffold. H2Sn could trigger a dramatic fluorescence enhancement (52-fold) with a fast response time and a low detection limit of 98.3 nM (S/N = 3). Moreover, JCCF was successfully applied to image H2Sn in living cells and zebra fish with low cytotoxicity.


Assuntos
Corantes Fluorescentes/química , Sulfeto de Hidrogênio/análise , Sulfetos/análise , Animais , Corantes Fluorescentes/análise , Humanos , Limite de Detecção , Células MCF-7 , Microscopia Confocal/métodos , Espectrometria de Fluorescência/métodos , Peixe-Zebra
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 226: 117635, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31605973

RESUMO

Hydrogen sulfide (H2S) has been found to be an important biological regulator that plays important roles in many physiological and pathological processes. Near-infrared (NIR) fluorescent probes capable of selectively detecting H2S in vivo will be useful tools to understand its mechanisms in biological processes. Herein, we reported an easily synthesized and stimuli-responsive NIR fluorescent probe (QCy7-HS) for selective evaluation of endogenous H2S in the living cells and mice. In response to cellular H2S stimulus, QCy7-HS is converted to QCy7 and shows a unique off-on near-infrared fluorescence signal change. The results of selectivity and kinetic studies indicated that our probe has high H2S binding capacity. Therefore, this probe was used for the fluorescence detection of H2S in cells. Moreover, the probe was applied for study exogenous and endogenous H2S in live mice, indicating that the new probe can be used as an efficient tool on H2S related events in living animals.


Assuntos
Corantes Fluorescentes/química , Corantes Fluorescentes/síntese química , Sulfeto de Hidrogênio/análise , Imagem Molecular/métodos , Animais , Feminino , Células HeLa , Células Hep G2 , Humanos , Teste de Materiais , Camundongos , Camundongos Endogâmicos BALB C , Microscopia de Fluorescência/métodos , Análise de Célula Única/métodos , Espectroscopia de Luz Próxima ao Infravermelho/métodos
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