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1.
Artigo em Chinês | MEDLINE | ID: mdl-33535342

RESUMO

Objective: To establish a method for the determination of bisphenol S in urine using dispersive liquid-liquid microextraction (DLLME) coupled with high performance liquid chromatography (HPLC) . Methods: The acetonitrile, octanol were used as extraction solvent, dispersive solvent respectively, for the preconcentration of bisphenol S. The optimal extraction conditions were optimized by single factor rotations, and methodological performance index were tested. Results: The linear correlation coefficient of bisphenol S in the range of 0.0-160 µg/L is greater than 0.999. The detection limit of this method was 0.76 µg/L, and the recovery rates were 88.06%-103.81%. The intra-and inter-day precisions were 1.78%-2.85% and 2.65%-4.25%, respectively. Conclusion: The method is reliable and sensitive. It is suitable for the determination of bisphenol S in urine samples for occupational exposure populations and non-professional.


Assuntos
Microextração em Fase Líquida , Cromatografia Líquida de Alta Pressão , Limite de Detecção , Fenóis , Solventes , Sulfonas
3.
Water Sci Technol ; 83(1): 198-211, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33460418

RESUMO

Blended organic copolymer (or homopolymer) and inorganic nanoparticles have been widely used (separately or simultaneously) for optimizing membrane pore structure and surface functionality. However, the prepared membranes suffer from degraded stability and insufficient integrity due to the high solubility or incompatibility of the blending additives. In this work, an organic-inorganic nanocomposite (i.e., PLA-TiO2) was designed, and employed for PSF membrane preparation. The Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analysis confirmed that bidentate chelating dominated the bonding mechanism between PLA and TiO2. The resultant PSF/PLA-TiO2 membranes possessed a highly porous surface with narrowed pore size distribution, demonstrating the strong pore forming ability of PLA-TiO2 for membrane preparations. Moreover, owing to the distinct inorganic-organic molecular conformation, the PLA-TiO2 exhibited enhanced stability and dispersibility within the PSF substance, which endowed the membrane with long-acting hydrophilicity and UV responsiveness. Given the UV responsiveness that is introduced by PLA-TiO2, UV-assisted strategies (UV-F and UV-C) were designed to further mitigate membrane fouling. The fouling analysis indicated that both reversible fouling and irreversible fouling were reduced in the UV-C process, signifying the synergistic effect between photocatalysis and hydraulics in membrane fouling mitigation. The enhanced membrane performance and the efficient preparation process highlight the potential of PLA-TiO2 in membrane modifications.


Assuntos
Nanocompostos , Polímeros , Poliésteres , Sulfonas , Titânio
4.
Chemosphere ; 267: 128871, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33308836

RESUMO

Despite the huge contribution of membrane-based brine and wastewater purification systems in today's life, biofouling still affects sustainability of membrane engineering. Aimed at reducing membrane modules wastage, the need to study biofouling monitoring as one of contributory factors stemmed from the short time between initial attachment and irreversible biofoulant adhesion. Hence, a membrane for monitoring is introduced to determine the right cleaning time by using fluorescent sensing as a non-destructive and scalable approach. The classical solid-state emissive fluorophore, tetraphenylethylene (TPE), was introduced as a sustainable, safe and sensitive fluorescent indicator in order to show the potential of the method, and polyethersulfone (PES) and nonsolvent-induced phase separation method, the most popular material and method, are used to fabricate membrane in industry and academia. Since the employed filler has an aggregation-induced emission (AIE) characteristic, it can track the biofouling throughout the operation. The fabricated membranes have certain characterizations (i.e. morphology assessment, flux, antibiogram, flow cytometry, surface free energy, and protein adsorption) which indicate that hybrid membrane with 5 wt % of TPE has identical biofouling activity compared to neat PES membrane and its optimal luminescence properties make it an appropriate candidate for non-destructive and online biofouling monitoring.


Assuntos
Incrustação Biológica , Purificação da Água , Luminescência , Membranas Artificiais , Polímeros , Estilbenos , Sulfonas , Ultrafiltração
5.
Environ Pollut ; 270: 116243, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33326921

RESUMO

BACKGROUND: Bisphenol S is an endocrine disruptor exhibiting metabolic disturbances, especially following perinatal exposures. To date, no data are available on the obesogen effects of BPS in a mutligenerational issue. OBJECTIVES: We investigated obesogen effects of BPS in a multigenerational study by focusing on body weight, adipose tissue and plasma parameters in male and female mice. METHODS: Pregnant C57BL6/J mice were exposed to BPS (1.5 µg/kg bw/day ie a human equivalent dose of 0.12 µg/kg bw/day) by drinking water from gestational day 0 to post natal day 21. All offsprings were fed with a high fat diet during 15 weeks. Body weight was monitored weekly and fat mass was measured before euthanasia. At euthanasia, blood glucose, insuline, triglyceride, cholesterol and no esterified fatty acid plasma levels were determined and gene expressions in visceral adipose tissue were assessed. F1 males and females were mated to obtain the F2 generation. Likewise, the F2 mice were cross-bred to obtain F3. The same analyses were performed. RESULTS: In F1 BPS induced an overweight in male mice associated to lipolysis gene expressions upregulation. In F1 females, dyslipidemia was observed. In F2, BPS exposure was associated to an increase in body weight, fat and VAT masses in males and females. Several plasma parameters were increased but with a sex related pattern (blood glucose, triglycerides and cholesterol in males and NEFA in females). We observed a down-regulation in mRNA expression of gene involved in lipogenesis and in lipolysis for females but only in the lipogenesis for males. In F3, a decrease in VAT mass and an upregulation of lipogenesis gene expression occurred only in females. CONCLUSIONS: BPS perinatal exposure induced sex-dependent obesogen multigenerational effects, the F2 generation being the most impacted. Transgenerational disturbances persisted only in females.


Assuntos
Dieta Hiperlipídica , Efeitos Tardios da Exposição Pré-Natal , Animais , Dieta Hiperlipídica/efeitos adversos , Feminino , Camundongos , Fenóis/toxicidade , Gravidez , Sulfonas
6.
Sci Total Environ ; 750: 141633, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32882496

RESUMO

Bisphenol S (BPS) is increasingly used in a wide range of industrial and consumer products, resulting in its ubiquitous distribution across the environment, including aquatic ecosystems. Although it is commonly known as a weak/moderate estrogenic compound, there has been a growing acknowledgment of the potential of BPS to cause toxicity by inducing oxidative stress. Oxidative stress is a major participant in the development of anxiety-like behaviors in humans and animals. Therefore, the present study was designed to examine the impact of BPS on anxiety-like behavior and fear responses in adult zebrafish and also to elucidate the possible linkage between the BPS neurotoxicity and oxidative status of the brain. To this end, adult male and female zebrafish were exposed to 0 (control), 1, 10, and 30 µg/L of BPS and 1 µg/L of 17-ß-estradiol (E2) for 75 days. Following exposure, changes in anxiety and fear-related responses were evaluated by applying a novel tank test and by exposing focal fish to chemical alarm cues. Additionally, we evaluated the expression of multiple antioxidant genes in the zebrafish brain. Our results indicate that BPS, irrespective of exposure concentration, and E2 significantly decreased bottom-dwelling behavior and the latency to enter the upper water column. Furthermore, exposure to the highest concentration of BPS and E2 induced a significant decrease in fear-related responses. The impaired anxiety and reduced fear-related responses were associated with a down-regulation in the transcription of genes involved in enzymatic antioxidant defense. Taken together, our results suggest that chronic exposure to BPS impairs anxiety and fear responses in adult zebrafish, possibly by inducing oxidative stress in the brain.


Assuntos
Poluentes Químicos da Água , Peixe-Zebra , Animais , Ansiedade/induzido quimicamente , Ecossistema , Medo , Feminino , Humanos , Masculino , Estresse Oxidativo , Fenóis , Sulfonas , Poluentes Químicos da Água/toxicidade
7.
Sci Total Environ ; 753: 141949, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-32891999

RESUMO

Bisphenol S (BPS) is the major substitute for the production of bisphenol A (BPA)-free products and detected in both food and environment. Although the relationship between BPA exposure and increased risk of obesity and diabetes has been noted, the potential influence of BPS is not fully understood. Herein, a non-targeted lipidomic study was performed to explore BPA/BPS exposure actions using the 3T3-L1 preadipocyte differentiation model, and revealed the comprehensive lipidome disturbance induced by either BPA or BPS exposure at different doses of 0.01, 1 and 100 µM. BPA was more potent than BPS in disturbance of lipid metabolism. A considerable similarity of BPS exposure to BPA was discovered. The key lipid remodeling in response to exposure was found to involve the cardiolipins, phosphatidylglycerols and fatty acids metabolic pathways, providing novel clues of potential mechanism in which both BPA and BPS exposure could be associated with increased risk of insulin resistance. Our study supplies the perspective into the lipidome response to environmental stress induced by BPA/BPS, and shows that BPA-free products are not necessarily safer. Substitution of BPA by its structural analog BPS should be therefore performed with caution.


Assuntos
Compostos Benzidrílicos , Lipidômica , Animais , Compostos Benzidrílicos/toxicidade , Camundongos , Fenóis/toxicidade , Sulfonas
8.
Chemosphere ; 262: 128045, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33182117

RESUMO

The vulnerability to environmental insults is heightened at early stages of development. However, the neurotoxic potential of bisphenol A (BPA) and bisphenol S (BPS) at developmental windows remains unclear. To investigate the mechanisms mediating the developmental neurotoxicity, zebrafish embryos were treated with 0.01, 0.03, 0.01, 0.3, 1 µM BPA/BPS. Also, we used Tg(HuC:GFP) zebrafish to investigate whether BPA/BPS could induce neuron development. The reduction in body length, and increased heart rate were significant in 0.3 and 1 µM BPA/BPS groups. The green fluorescence protein (GFP) intensity increased at 72 hpf and 120 hpf in Tg(HuC:GFP) larvae which was consistent with the increased mRNA expression of elval3 following BPS treatments, an indication of the plausible effect of BPS on embryonic neuron development. Additionally, BPA/BPS treatments elicited hyperactivity and reduced static time in zebrafish larvae, suggesting behavioral alterations. Moreover, qRT-PCR results showed that BPA and BPS could interfere with the normal expression of development-related genes vegfa, wnt8a, and mstn1 at the developmental stages. The expression of neurodevelopment-related genes (ngn1, elavl3, gfap, α1-tubulin, mbp, and gap43) were significantly upregulated in BPA and BPS treatments, except for the remarkable downregulation of mbp and gfap elicited by BPA at 48 (0.03 µM) and 120 hpf (0.3 µM) respectively; ngn1 at 48 hpf for 0.1 µM BPS. Overall, our results highlighted that embryonic exposure to low concentrations of BPA/BPS could be deleterious to the central nervous system development and elicit behavioral abnormalities in zebrafish at developmental stages.


Assuntos
Compostos Benzidrílicos/toxicidade , Embrião não Mamífero/efeitos dos fármacos , Desenvolvimento Embrionário/efeitos dos fármacos , Neurogênese/efeitos dos fármacos , Fenóis/toxicidade , Sulfonas/toxicidade , Poluentes Químicos da Água/toxicidade , Peixe-Zebra , Animais , Embrião não Mamífero/metabolismo , Larva/efeitos dos fármacos , Larva/metabolismo , Peixe-Zebra/metabolismo , Proteínas de Peixe-Zebra/metabolismo
9.
Chemosphere ; 262: 128009, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33182144

RESUMO

Increasing evidence has highlighted the critical role of early life environment in shaping the future health outcomes of individuals in subsequent generations. Bisphenol S (BPS) has been widely used as a substitute for various plastic materials due to the limited application of Bisphenol A (BPA) which is an endocrine disruptor. However, the lack of efficient evaluation of BPS leaves doubts about the relevant substitute of BPA. Few studies of transgenerational inheritance have examined the effects of environmental exposures to endocrine disruptors on the immune system. In this study, we analyzed the transgenerational effects of BPS on intestinal inflammation and its consequence in metabolism. In this study, only F0 pregnant mice were exposed to BPS (1.5 µg/kg bw/day) from gestational day 0 until weaning of offspring. In this work, both F1 and F2 male offspring developed an inflammatory response in the ileum and colon at adulthood after F0 mothers were exposed to BPS; this phenomenon disappeared in F3. This inflammatory response in F1 male offspring is associated with a significant decrease of blood cholesterol without modification of metabolic status. Further, in F3 offspring male, the decrease of gut inflammatory response is associated with a decrease of fat weight and with an increase of blood glucose and cholesterol level. A sex-specific profile is observed in female offspring. We also observed that early life exposure to BPS was associated with strong abnormal intestinal immune status. The study presented here demonstrates that the immune system, like other organ systems, is vulnerable to transgenerational effects caused by environmental exposures.


Assuntos
Citocinas/análise , Disruptores Endócrinos/toxicidade , Intestinos/imunologia , Fenóis/toxicidade , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Sulfonas/toxicidade , Animais , Glicemia/análise , Peso Corporal/efeitos dos fármacos , Fezes/química , Feminino , Inflamação , Intestinos/crescimento & desenvolvimento , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Gravidez , Efeitos Tardios da Exposição Pré-Natal/imunologia
10.
Chemosphere ; 263: 127892, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32822943

RESUMO

Nitrogen-doped porous graphene oxide (N-PGO) was synthesized, characterized, and applied as a hydrophilic nanomaterial in fabrication of polyethersulfone (PES) membrane for Reactive Red 195 dye and bovine serum albumin (BSA) protein separation. The N-PGO nanosheets not merely showed a good adhesion towards polymers, but simultaneously promoted hydrogen bonding action. Therefore, high-efficiency permeation passageway in the separation layer of membranes was attained. X-ray photoelectron spectroscopy (XPS), energy dispersive spectroscopy (EDX) and Fourier transform infra-red spectroscopy (FTIR) analyses approved nitrogen doping, which increased hydrophilicity and hydrogen bonding ability of PGO in water filtration. The pure water permeation of nanocomposite membranes could reach as high as 190 L m-2 h-1 at 3 bar. A dye rejection efficiency higher than 92% and BSA rejection higher than 95% were accordingly obtained. Atomic force microscopy (AFM) images approved formation of a rough surface that was decreased by addition of low amounts of the PGO. SEM images provided from the surface also confirmed enlarged pore size and increased porosity. Antifouling properties were investigated by BSA filtration, and results showed that the flux recovery ratio of the N-PGO membrane was improved. Overall, the N-PGO hybrid membranes exhibited potential for application in separation of typical proteins and dyes with good antifouling properties.


Assuntos
Corantes/química , Nanocompostos/química , Poluentes Químicos da Água/química , Purificação da Água/métodos , Animais , Bovinos , Corantes/análise , Filtração , Grafite/química , Ligação de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Membranas Artificiais , Nitrogênio , Polímeros/química , Porosidade , Soroalbumina Bovina/química , Sulfonas , Água/química , Poluentes Químicos da Água/análise
11.
Food Chem ; 339: 127813, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32916401

RESUMO

Bisphenol S (BPS), a structural analog of Bisphenol A (BPA), has been widely used as a substitute for epoxy resin, food packaging materials, and other products due to the limited application of BPA. Studies in vivo and in vitro have indicated that BPA could induce fat accumulation like an obesogen. The main goal of this study was to investigate the role and mechanism of BPS in lipid metabolism using Caenorhabditis elegans (C. elegans) as a model. Results showed that both the overall fat deposition and the triglyceride level were significantly increased in a non-monotonically increasing trend, and the low dose of BPS (0.01 µM) exhibited a stronger influence. Additionally, BPS enhanced fat synthesis depending on daf-16, fat-5, fat-6 and fat-7, and inhibited fatty acid oxidation via nhr-49 and acs-2. This study further indicate that fat accumulation induced by BPS requires nhr-49, which also mediated the nuclear hormone signaling pathway.


Assuntos
Caenorhabditis elegans/efeitos dos fármacos , Glucose/efeitos adversos , Metabolismo dos Lipídeos/efeitos dos fármacos , Fenóis/toxicidade , Sulfonas/toxicidade , Animais , Animais Geneticamente Modificados , Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/fisiologia , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Dieta/efeitos adversos , Gorduras/metabolismo , Ácidos Graxos/metabolismo , Glucose/administração & dosagem , Receptores Citoplasmáticos e Nucleares/genética , Receptores Citoplasmáticos e Nucleares/metabolismo , Triglicerídeos/metabolismo
12.
Ecotoxicol Environ Saf ; 207: 111299, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32927158

RESUMO

The analogues of biphenol A (BPA), including bisphenol S (BPS) and bisphenol B (BPB), are commonly used to replace the application of BPA in containers and wrappers of daily life. However, their safeties are questioned due to their similar chemical structure and possible physiological effects as BPA. To investigate the neurotoxic effects of BPA, BPS, and BPB as well as their underlying mechanism, IMR-32 cell line from male and SK-N-SH cell line from female were exposed respectively to BPA, BPS and BPB with concentrations of 1 nM, 10 nM, 100 nM, 1 µM, 10 µM, and 100 µM for 24 h. Additionally, 24 h exposure of BPA combining epigallocatechin gallate (EGCG) (4 µM and 8 µM for IMR-32 and SK-N-SH respectively) were conducted. Results demonstrated that BPs exposure could promote reactive oxygen species production and increase level of malondialdehyde (MDA) while decrease levels of superoxide dismutase (SOD). Intensive study revealed that after exposure to BPA mitochondrial membrane potential (MMP) dropped down and the protein expression levels of Bak-1, Bax, cytochrome c and Caspase-3 were up-regulated but Bcl-2 were down-regulated significantly. Moreover, apoptosis rate was raised and cell activity declined remarkably in the neuroblastoma cells. All the effects induced by BPA could be alleviated by the adding of EGCG, which similar alleviations could be inferred in IMR-32 and SK-N-SH cells induced by BPS and BPB. Furthermore, BPS showed lower neurotoxic effects compared to BPA and BPB. Interestingly, the neurotoxic effects of BPA on IMR-32 cells were significantly higher than those on SK-N-SH cells. In conclusion, the results suggested that BPA, BPS and BPB could induce oxidative stress and apoptosis via mitochondrial pathway in the neuroblastoma cells and male is more susceptible to BPs than female.


Assuntos
Apoptose/efeitos dos fármacos , Compostos Benzidrílicos/toxicidade , Disruptores Endócrinos/toxicidade , Mitocôndrias/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Fenóis/toxicidade , Sulfonas/toxicidade , Caspase 3/metabolismo , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Feminino , Humanos , Masculino , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Caracteres Sexuais
13.
Water Res ; 188: 116530, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33125997

RESUMO

Membranes have a lot of potential for harvesting microalgae, but membrane fouling is hampering their breakthrough. In this study, the effects of charge and corrugated surface on membrane filtration performance were investigated. The clean water permeance (CWP), the microalgae harvesting efficiency and the membrane flux for a microalgal broth were determined using patterned polysulfone (PSf) membranes with different shapes of the surface patterns and containing different charge densities by blending sulfonated polysulfone (sPSf). The flow behavior near the patterned membrane surface, as well as the interaction energy between membrane and microalgae were investigated using computational fluid dynamics (CFD) simulation and the improved extended "Derjaguin, Landau, Verwey, Overbeek" (XDLVO) theory, respectively. Membrane charge and pattern shape significantly improve the membrane performance. The critical pressures of all sPSf blend patterned membranes were higher than 2.5 bar. A 4.5w% sPSf blend patterned membranes with wave patterns showed the highest CWP (2300 L/m2 h bar) and membrane flux in the microalgal broth (1000 L/m2 h bar) with 100% harvesting efficiency. XDLVO analysis showed that sPSf blend patterned membranes prepared obtained the lowest interaction energy and highest energy barrier for microalgal attachment. CFD simulation showed a higher velocity and wall shear on the pattern apexes.


Assuntos
Microalgas , Biomassa , Filtração , Membranas , Membranas Artificiais , Polímeros , Sulfonas
14.
Sci Total Environ ; 762: 144059, 2021 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-33360459

RESUMO

Bisphenol S (BPS) is now used as an alternative of bisphenol A (BPA), but has been implicated in male reproductive dysfunction-including diminished sperm number and quality and altered hormonal concentrations. However, the mechanisms of action subserving these effects remains unclear. In the present study, BPS at doses of 50 mg/kg bw and 100 mg/kg bw caused defects in the integrity of the blood-testis barrier (BTB) and apical ectoplasmic specialization (ES), and we also delineated an underlying molecular mechanism of action. BPS induced F-actin and α-tubulin disorganization in seminiferous tubules, which in turn led to the truncation of actin filaments and microtubules. Additionally, BPS was found to perturb the expression of the actin-binding proteins Arp3 and Eps8, which are critical for the organization of the actin filaments. mTORC1 and mTORC2 manifest opposing roles in Sertoli cell junctional function, and we demonstrated that mTORC1/rpS6/Akt/MMP9 signaling was increased and that mTORC2/rictor activity was also attenuated. In summary, we showed that BPS-induced disruption of the BTB and apical ES perturbed normal spermatogenic function that was mediated by mTORC1 and mTORC2. The imbalance in mTORC1 and mTORC2, in turn, altered the expression of actin-binding proteins, resulting in the impairment of F-actin and MT organization, and inhibited the expression of junctional proteins at the BTB and apical ES.


Assuntos
Barreira Hematotesticular , Espermatogênese , Proteínas Adaptadoras de Transdução de Sinal , Animais , Junções Intercelulares , Masculino , Alvo Mecanístico do Complexo 1 de Rapamicina , Alvo Mecanístico do Complexo 2 de Rapamicina , Fenóis , Ratos , Sulfonas
15.
Chemosphere ; 263: 128304, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33155548

RESUMO

Gap junction intercellular communication (GJIC) is necessary for ovarian function, and it is temporospatially regulated during follicular development and ovulation. At outermost layer of the antral follicle, theca cells provide structural, steroidogenic, and vascular support. Inter- and extra-thecal GJIC is required for intrafollicular trafficking of signaling molecules. Because GJIC can be altered by hormones and endocrine disrupting chemicals (EDCs), we tested if any of five common EDCs (bisphenol A (BPA), bisphenol S (BPS), bisphenol F (BPF), perfluorooctanesulfonic acid (PFOS), and triphenyltin chloride (TPT)) can interfere with theca cell GJIC. Since most chemicals are reported to repress GJIC, we hypothesized that all chemicals tested, within environmentally relevant human exposure concentrations, will inhibit theca cell GJICs. To evaluate this hypothesis, we used a scrape loading/dye transfer assay. BPS, but no other chemical tested, enhanced GJIC in a dose- and time-dependent manner in ovine primary theca cells. A signal-protein inhibitor approach was used to explore the GJIC-modulatory pathways involved. Phospholipase C and mitogen-activated protein kinase (MAPK) inhibitors significantly attenuated BPS-induced enhanced GJIC. Human theca cells were used to evaluate translational relevance of these findings. Human primary theca cells had a ∼40% increase in GJIC in response to BPS, which was attenuated with a MAPK inhibitor, suggestive of a conserved mechanism. Upregulation of GJIC could result in hyperplasia of the theca cell layer or prevent ovulation by holding the oocyte in meiotic arrest. Further studies are necessary to understand in vitro to in vivo translatability of these findings on follicle development and fertility outcomes.


Assuntos
Substâncias Perigosas/toxicidade , Fenóis/toxicidade , Sulfonas/toxicidade , Células Tecais/fisiologia , Animais , Compostos Benzidrílicos , Comunicação Celular , Comunicação , Conexina 43/metabolismo , Feminino , Junções Comunicantes/metabolismo , Humanos , Oócitos/metabolismo , Ovinos , Transdução de Sinais , Células Tecais/efeitos dos fármacos , Células Tecais/metabolismo
16.
Chemosphere ; 263: 127902, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33297008

RESUMO

Bis(4-chlorophenyl) sulfone (BCPS, CAS No. 80-07-9) is used as monomer for the production of several groups of polymers like polysulphones and polyethersulphones. Residual amounts of monomer remain in the polymer matrix and might migrate out from the polymer matrix. In the present study, freshwater fish and fish-eating birds were examined. Following fish species (top predators) were collected at two Austrian locations: Sander lucioperca, Silurus glanis, and Lota lota. Whole fish samples were analysed for BCPS. Levels in freshwater fish ranged between 1.3 and 9.3 ng/g fat. In addition, breast muscle and liver samples from six cormorants (Phalacrocorax carbo sinensis) were investigated. BCPS levels in cormorants breast muscle were in the range of 4.3-40 ng/g fat (mean: 16.3 ng/g fat, n = 6) and 28-86 ng/g fat (mean: 53.5 ng/g fat, n = 6) in the liver samples. BCPS concentration in liver was 3.3-fold higher than in muscle tissue. One of the cormorants had ingested fish with a BCPS level of 5.5 ng/g fat; BCPS levels in the cormorant were 23 ng/g fat (breast muscle) and 28 ng/g fat (liver), suggesting biomagnification values (BMF) of 4.2 (fish/breast muscle) and 5.1 (fish/liver), respectively. A BMF value higher than 1 can be considered as an indication for very high biomagnification. Comparing the BCPS concentrations of cormorants' breast muscle from 2019 (mean: 16 ng/g fat) to the concentrations from 2001 to 2005 (mean: 8.9 ng/g fat), indicates that BCPS levels might be increasing in Europe.


Assuntos
Bioacumulação , Aves , Animais , Áustria , Europa (Continente) , Água Doce , Projetos Piloto , Sulfonas
17.
Chemosphere ; 263: 128019, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33297043

RESUMO

Bisphenol S (BPS) is a main substitute for bisphenol A, which are ubiquitous in human daily products. Newborn mitochondrial DNA copy number (mtDNAcn) is considered as a marker for biological aging and human health, and has been related to diseases in later life. We recruited 762 mother-newborn pairs in a birth cohort study between 2013 and 2015 in Wuhan, China. Urinary BPS concentrations were detected using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). MtDNAcn from cord blood was measured by quantitative real-time polymerase chain reaction (qPCR). We applied multiple informant models based on generalized estimating equations to assess the associations between prenatal BPS exposure and mtDNAcn. The median urine concentrations of BPS were 0.32 µg/L, 0.34 µg/L, and 0.36 µg/L in the first, second, and third trimesters, respectively. In the multiple informant models, we observed significant associations between BPS and mtDNAcn among male newborns. Compared with the lowest quarters, the second, third, and the highest quarter of BPS level were associated with 58.00% (95% CI: 76.58%, -24.66%), 64.65% (95% CI: 79.40%, -39.33%) and 59.07% (95% CI: 75.16%, -32.58%) reductions of mtDNAcn in the first trimester, respectively. No significant associations were found in the second and third trimesters. The associations between BPS and mtDNAcn were not found among female newborns. Findings from this study suggested that BPS exposure was related to decreased mtDNAcn in male newborns. The first trimester was identified as the critical windows for BPS exposure during pregnancy.


Assuntos
DNA Mitocondrial , Efeitos Tardios da Exposição Pré-Natal , China , Cromatografia Líquida , Estudos de Coortes , Variações do Número de Cópias de DNA , DNA Mitocondrial/genética , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Exposição Materna , Fenóis , Gravidez , Sulfonas , Espectrometria de Massas em Tandem
18.
Chemosphere ; 263: 128088, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33297086

RESUMO

Highly ordered periodic mesoporous organosilica (PMO) with large-pores (9.3 nm) synthesized using novel and cost-effective route. The synthesized PMO was then functionalized by p-phenylenediamine (PPD) and incorporated in polyethersulfone (PES) nanofiltration membrane| with various dosage using phase inversion method. The prepared membranes were characterized with FT-IR/ATR, FE-SEM and EDX techniques. The overall porosity, the mean of pore radius, water contact angle, fouling, permeation, and rejection were computed. As a result, the hydrophilicity and the pure water flux of membranes were significantly enhanced after modification with PMO-PPD due to its high hydrophilic nature as nanofiller. Ultimately, the modified membrane with 0.25 wt% of PPD was found the ideal membrane with water contact angle of 54.2%, pure water flux of 33.7 L/m2 h, porosity of 74.1%, and mean pore radius of 4.96 nm. This membrane showed the maximum increase in water flux and superlative anti-fouling growth when compared to the other studied membranes. Moreover, among modified membranes with various quantity of PPD, the performance of the PES-PMO-PPD 0.25 wt% was the best for rejection of MO dye (96.7%), Pb(II) (93.1%), Na2SO4 (73.0%), MgSO4 (41.6%), and NaCl (33.7%).


Assuntos
Metais Pesados , Águas Residuárias , Membranas Artificiais , Fenilenodiaminas , Polímeros , Sais , Espectroscopia de Infravermelho com Transformada de Fourier , Sulfonas
19.
Biomed Pharmacother ; 133: 111073, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33378972

RESUMO

Sepsis is a life-threatening condition often leading to multiple organ failure for which currently no pharmacological treatment is available. Endothelial cells (EC) are among the first cells to respond to pathogens and inflammatory mediators in sepsis and might be a sentinel target to prevent the occurrence of multiple organ failure. Lipopolysaccharide (LPS) is a Gram-negative bacterial component that induces endothelial expression of inflammatory adhesion molecules, cytokines, and chemokines. This expression is regulated by a network of kinases, the result of which in vivo enables leukocytes to transmigrate from the blood into the underlying tissue, causing organ damage. We hypothesised that besides the known kinase pathways, other kinases are involved in the regulation of EC in response to LPS, and that these can be pharmacologically targeted to inhibit cell activation. Using kinome profiling, we identified 58 tyrosine kinases (TKs) that were active in human umbilical vein endothelial cells (HUVEC) at various timepoints after stimulation with LPS. These included AXL tyrosine kinase (Axl), focal adhesion kinase 1 (FAK1), and anaplastic lymphoma kinase (ALK). Using siRNA-based gene knock down, we confirmed that these three TKs mediate LPS-induced endothelial inflammatory activation. Pharmacological inhibition with FAK1 inhibitor FAK14 attenuated LPS-induced endothelial inflammatory activation and leukocyte adhesion partly via blockade of NF-κB activity. Administration of FAK14 after EC exposure to LPS also resulted in inhibition of inflammatory molecule expression. In contrast, inhibition of ALK with FDA-approved inhibitor Ceritinib attenuated LPS-induced endothelial inflammatory activation via a pathway that was independent of NF-κB signalling while it did not affect leukocyte adhesion. Furthermore, Ceritinib administration after start of EC exposure to LPS did not inhibit inflammatory activation. Combined FAK1 and ALK inhibition attenuated LPS-induced endothelial activation in an additive manner, without affecting leukocyte adhesion. Summarising, our findings suggest the involvement of FAK1 and ALK in mediating LPS-induced inflammatory activation of EC. Since pharmacological inhibition of FAK1 attenuated endothelial inflammatory activation after the cells were exposed to LPS, FAK1 represents a promising target for follow up studies.


Assuntos
Quinase do Linfoma Anaplásico/antagonistas & inibidores , Anti-Inflamatórios/farmacologia , Quinase 1 de Adesão Focal/antagonistas & inibidores , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Inflamação/prevenção & controle , Lipopolissacarídeos/toxicidade , Inibidores de Proteínas Quinases/farmacologia , Aminopiridinas/farmacologia , Quinase do Linfoma Anaplásico/genética , Quinase do Linfoma Anaplásico/metabolismo , Quinase 1 de Adesão Focal/genética , Quinase 1 de Adesão Focal/metabolismo , Perfilação da Expressão Gênica , Células HL-60 , Células Endoteliais da Veia Umbilical Humana/enzimologia , Humanos , Inflamação/enzimologia , Inflamação/genética , Análise Serial de Proteínas , Proteínas Proto-Oncogênicas/antagonistas & inibidores , Proteínas Proto-Oncogênicas/genética , Proteínas Proto-Oncogênicas/metabolismo , Piridonas/farmacologia , Pirimidinas/farmacologia , Receptores Proteína Tirosina Quinases/antagonistas & inibidores , Receptores Proteína Tirosina Quinases/genética , Receptores Proteína Tirosina Quinases/metabolismo , Transdução de Sinais , Sulfonas/farmacologia , Fatores de Tempo , Transcriptoma
20.
Chemosphere ; 268: 129306, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33360002

RESUMO

To extend the use of polyethersulfone (PES) ultrafiltration membranes in water process engineering, the membrane's wettability and anti-fouling properties should be further improved. In this context, hydroxyapatite/boron nitride (HAp/BN) nanocomposites have been prepared and intercalated into PES membranes using a non-solvent-induced phase separation process. High-quality 2D transparent boron nitride nanosheets (BN NSs) were prepared using an environmentally friendly and green-template assisted synthesis method in which 1D hexagonal hydroxyapatite nanosheets (HAp NRs) were uniformly distributed and hydrothermally immobilized at 180 °C. SEM, XRD, and Raman spectroscopy techniques were used to characterize the HAp/BN nanocomposites. PES membranes intercalated with various nanocomposite amounts (0-4 wt %) were also characterized by permeability, porosity, and contact angle measurements. Additional pathways for water molecule transport were promoted by the high surface area of the BN NSs, resulting in high permeability. Membrane wettability and antifouling properties were also improved by the inclusion of negative charge groups (OH- and PO43-) on HAp. Hybrid membranes containing 4 wt% HAp/BN showed the best overall performance with ∼97% increase in water flux, 90% rejection of bovine serum albumin (BSA), high water flux recovery ratio, low irreversible fouling, and high reversible fouling pattern. The intercalation of HAp/BN with the PES matrix therefore opens up a new direction to enhance the PES UF membranes' hydrophilicity, water flux, and antifouling capacity.


Assuntos
Nanocompostos , Ultrafiltração , Compostos de Boro , Durapatita , Membranas Artificiais , Permeabilidade , Polímeros , Sulfonas
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