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1.
Chemosphere ; 262: 127793, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32799142

RESUMO

Currently, few studies have investigated the joint toxicity mechanism of azole fungicides at different exposure times and mixed at the relevant environmental concentrations. In this study, three common azole fungicides, namely, myclobutanil (MYC), propiconazole (PRO), and tebuconazole (TCZ), were used in studying the toxic mechanisms of a single substance and its ternary mixture exposed to ambient concentrations of Chlorella pyrenoidosa. Superoxide dismutase (SOD), catalase (CAT), chlorophyll a (Chla), and total protein (TP), were used as physiological indexes. Results showed that three azole fungicides and ternary mixture presented obvious time-dependent toxicities at high concentrations. MYC induced a hormetic effect on algal growth, whereas PRO and TCZ inhibit algal growth in the entire range of the tested concentrations. The toxicities of the three azole fungicides at 7 days followed the order PRO > TCZ > MYC. Three azole fungicides and their ternary mixture induced different levels of SOD and CAT activities in algae at high concentrations. The ternary mixture showed additive effects after 4 and 7 days exposure, but no effect was observed at actual environmental concentrations. The toxic mechanisms may be related to the continuous accumulation of reactive oxygen species, which not only affected protein structures and compositions but also damaged thylakoid membranes, hindered the synthesis of proteins and chlorophyll a, and eventually inhibited algal growth. These findings increase the understanding of the ecotoxicity of azole fungicides and use of azole fungicides in agricultural production.


Assuntos
Antioxidantes/metabolismo , Azóis/toxicidade , Chlorella/efeitos dos fármacos , Fungicidas Industriais/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Catalase/metabolismo , Chlorella/enzimologia , Chlorella/crescimento & desenvolvimento , Clorofila A/metabolismo , Relação Dose-Resposta a Droga , Nitrilos/toxicidade , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo , Triazóis/toxicidade
2.
Chemosphere ; 262: 128384, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33182105

RESUMO

Arsenic (As) polluted food chain has become a serious issue for the growth and development of humans, animals and plants. Nitric oxide (NO) or silicon (Si) may mitigate As toxicity. However, the combined application of NO and Si in mitigating As uptake and phytotoxicity in Brassica juncea is unknown. Hence, the collegial effect of sodium nitroprusside (SNP), a NO donor and Si application on B. juncea growth, gas exchange parameters, antioxidant system and As uptake was examined in a greenhouse experiment. Arsenic toxicity injured cell membrane as signposted by the elevated level of malondialdehyde (MDA) and hydrogen peroxide (H2O2), thus decreasing the growth of stressed plants. Moreover, As stress negatively affected gas exchange parameters and antioxidative system of plants. However, NO or/and Si alleviated As induced oxidative stress through increasing the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione S-transferase (GST), glutathione (GSH), along with thiol and proline synthesis. Furthermore, plants treated with co-application of NO and Si showed improved growth, gas attributes and decreased As uptake under As regimes. The current study highlights that NO and Si synergistically interact to mitigate detrimental effects of As stress through reducing As uptake. Our findings recommend combined NO and Si application in As spiked soils for improvement of plant growth and stress alleviation.


Assuntos
Arsênico/metabolismo , Mostardeira/fisiologia , Óxido Nítrico/química , Silício/química , Poluentes do Solo/metabolismo , Antioxidantes/metabolismo , Arsênico/toxicidade , Ascorbato Peroxidases/metabolismo , Glutationa/metabolismo , Glutationa Redutase/metabolismo , Peróxido de Hidrogênio/metabolismo , Malondialdeído/metabolismo , Mostardeira/metabolismo , Doadores de Óxido Nítrico/metabolismo , Doadores de Óxido Nítrico/farmacologia , Nitroprussiato/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Plântula/efeitos dos fármacos , Poluentes do Solo/toxicidade , Superóxido Dismutase/metabolismo
3.
Chemosphere ; 262: 127826, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33182120

RESUMO

The present manuscript investigates the roles of silicon nanoparticles (SiNPs) in ameliorating fluoride toxicity in the susceptible rice cultivar, IR-64. Fluoride toxicity reduced overall growth and yield by suppressing grain development. Fluoride stress alarmingly increased the accumulation of cobalt, which together with fluoride triggered electrolyte leakage, malondialdehyde, methylglyoxal and hydrogen peroxide accumulation and NADPH oxidase activity. The overall photosynthesis was compromised due to chlorosis and inhibited Hill activity. Nano-Si-priming efficiently ameliorated molecular injuries and restored yield by reducing fluoride bioaccumulation particularly in the grains. The level of non-enzymatic antioxidants like anthocyanins, flavonoids, phenolics and glutathione was stimulated upon SiNP-priming. Nano-Si-pulsing removed fluoride-mediated inhibition of glutathione synthesis by activating glutathione reductase. Glutathione was utilized to activate glyoxalases and associated enzymes like glutathione-S-transferase and glutathione peroxidase. Uptake of nutrients like silicon, potassium, zinc, copper, iron, nickel, manganese, selenium and vanadium improved seedling health even during prolonged fluoride stress. Nano-Si-pulsing produced a nanozymatic effect, since high level of crucial co-factors like copper, zinc and iron stimulated the activity of superoxide dismutase, catalase, ascorbate peroxidase and guaiacol peroxidase, which synergistically with other enzymatic and non-enzymatic antioxidants scavenged reactive oxygen species and promoted fluoride tolerance. Overall, the study supported by statistical modelling using principal component analysis, t-distributed stochastic neighbour embedding and multidimensional scaling, established the potential of SiNP to promote safe rice cultivation and precision farming even in fluoride-infested environments.


Assuntos
Fluoretos/toxicidade , Oryza/fisiologia , Silício/química , Poluentes Químicos da Água/toxicidade , Antioxidantes/metabolismo , Ascorbato Peroxidases , Catalase , Glutationa/metabolismo , Glutationa Peroxidase , Peróxido de Hidrogênio/metabolismo , Malondialdeído , Oryza/efeitos dos fármacos , Peroxidase , Espécies Reativas de Oxigênio/metabolismo , Plântula/efeitos dos fármacos , Superóxido Dismutase
4.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 32(10): 1199-1202, 2020 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-33198863

RESUMO

OBJECTIVE: To analyze the expression and clinical significance of serum retinol binding protein 4 (RBP4), superoxide dismutase (SOD) and hypersensitive C-reactive protein (hs-CRP) in patients with acute ST-segment elevated myocardial infarction (STEMI). METHODS: Seventy-eight patients with STEMI who underwent percutaneous coronary intervention (PCI) admitted to Nanxishan Hospital of Guangxi Zhuang Autonomous Region from January 2017 to March 2019 were enrolled. On the basis of the routine treatment, atorvastatin calcium or rosuvastatin calcium treatment was given. According to the results of coronary angiography, the patients were divided into three groups: single-vessel, double-vessel and three-vessel lesions, and the severity of coronary artery disease score (SYNTAX score) was performed. The serum RBP4, SOD and hs-CRP were measured and compared among different groups before and after treatment. The correlation between the three serum parameters of STEMI patients and the SYNTAX score, risk prediction (STEMI high-risk score) and prognosis were recorded and compared among different groups. RESULTS: There was no significant difference in serum RBP4, SOD and hs-CRP among different groups before treatment. In multivessel (double-vessel and three-vessel) vascular disease group, SYNTAX score was significantly correlated with RBP4 and hs-CRP (double-vessel lesions: r values were 0.616 and 0.489 respectively, three-vessel lesions: r values were 0.423 and 0.357 respectively, all P < 0.05), but had no correlation with SOD (r values were 0.108 and 0.055 respectively, both P > 0.05), and high-risk score was closely correlated with RBP4 and hs-CRP levels (r values were 0.581 and 0.623 respectively, both P < 0.01). There was no significant difference in the prognosis of patients treated with different statins. There were 8 and 7 high-risk patients in the atorvastatin calcium group and rosuvastatin calcium group, respectively, and 2 and 3 dead patients respectively (both P > 0.05). CONCLUSIONS: Combined detection of serum RBP4, SOD and hs-CRP levels can be used as better indicators to predict the risk of STEMI, which is worthy of clinical application.


Assuntos
Infarto do Miocárdio , Intervenção Coronária Percutânea , Proteína C-Reativa/análise , China , Humanos , Infarto do Miocárdio/tratamento farmacológico , Proteínas Plasmáticas de Ligação ao Retinol , Superóxido Dismutase
5.
Bull Environ Contam Toxicol ; 105(5): 711-714, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33001235

RESUMO

The dusky grouper Epinephelus marginatus is predator fish subjected to be impacted due to the contamination of their habitats. A viable source of metal contamination, i.e., copper (Cu), in this species is the ingestion of contaminated food. The objective of this work was to verify the toxic effects of Cu contaminated feed in dusky grouper. A 15 days trial was conducted with three treatments: control, 1 g and 2 g Cu/kg of fish feed. After the trial, the gut was analyzed for Cu concentration and the liver for SOD and GST activity. The Cu concentration in the intestinal tract was significantly greater in fish from contaminated treatments when compared with control. The SOD was significantly lower in contaminated fish, and the GST did not show differences among treatments. Copper showed to be toxic for the species, as evidenced by gut accumulation and suggested by SOD response.


Assuntos
Ração Animal , Cobre/toxicidade , Conteúdo Gastrointestinal/química , Perciformes/metabolismo , Poluentes Químicos da Água/toxicidade , Animais , Cobre/análise , Ecossistema , Fígado/efeitos dos fármacos , Fígado/enzimologia , Modelos Teóricos , Alimentos Marinhos , Superóxido Dismutase/metabolismo , Poluentes Químicos da Água/análise
6.
Chem Biol Interact ; 331: 109276, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33002459

RESUMO

Ulcerative colitis (UC) is a chronic disease driven primarily by uncontrolled pervasive inflammatory responses affecting the colon and rectum. Currently available medications carry multiple detrimental adverse effects, which have emphasized the mandatory need for safer and more efficient novel therapeutic alternatives. Melittin is the main constituent of bee venom and exhibits potent anti-inflammatory properties. The antiulcerogenic effect of oral melittin (40 µg/kg) was explored in the current study using the acetic acid-induced colitis model. Increase in body weight and decrease in colon mass index were observed in the melittin group. Microscopically, melittin ameliorated acetic acid-induced histological damage. Melittin administration has efficiently amended the elevated levels of the cytokines, tumor necrosis factor (TNF-α) and interleukin 6 (IL-6) seen in the colitis group. This was accompanied by inhibition of the upstream signaling molecules, Toll-like receptor 4 (TLR4), tumor necrosis factor receptor (TNF-R)-associated factor (TRAF6), mitogen-activated protein kinase 38 (p38 MAPK), and nuclear factor kappaB (NF-κB) in the melittin group. Moreover, treatment with melittin resulted in marked decrease in colonic level of prostaglandin E2 (PGE2) together with the enzymes involved in its synthesis, secretory phospholipase A2 (sPLA2) and cyclooxygenase 2 (COX-2). Additionally, melittin has attenuated acetic acid-induced oxidative stress as manifested by the significant diminishment in malondialdehyde (MDA) as well as the increase in superoxide dismutase (SOD) and reduced glutathione (GSH) levels. Therefore, melittin mitigated UC pathogenesis and could be considered as a potent and promising therapeutic alternative for UC treatment.


Assuntos
Antiulcerosos/farmacologia , Meliteno/farmacologia , Transdução de Sinais/efeitos dos fármacos , Fator 6 Associado a Receptor de TNF/metabolismo , Receptor 4 Toll-Like/metabolismo , Ácido Acético/toxicidade , Administração Oral , Animais , Antiulcerosos/uso terapêutico , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Colite Ulcerativa/patologia , Colo/metabolismo , Colo/patologia , Ciclo-Oxigenase 2/metabolismo , Interleucina-6/metabolismo , Malondialdeído/metabolismo , Meliteno/uso terapêutico , Camundongos , NF-kappa B/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
7.
PLoS One ; 15(9): e0239496, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32946513

RESUMO

Carnosine is a naturally occurring histidine-containing dipeptide present at high concentration in mammalian skeletal muscles. Carnosine was shown to affect muscle contraction, prevent the accumulation of oxidative metabolism by-products and act as an intracellular proton buffer maintaining the muscle acid-base balance. The present study was undertaken to gain additional knowledge about the intracellular mechanisms activated by carnosine in porcine myoblast cells under basal and oxidative stress conditions. Satellite cells were isolated from the skeletal muscles of 3 to 4 day-old piglets to study the effect of 0, 10, 25 and 50 mM carnosine pre-treatments in cells that were exposed (0.3 mM H2O2) or not to an H2O2-induced oxidative stress. Study results demonstrated that carnosine acts differently in myoblasts under oxidative stress and in basal conditions, the only exception being with the reduction of reactive oxygen species and protein carbonyls observed in both experimental conditions with carnosine pre-treatment. In oxidative stress conditions, carnosine pre-treatment increased the mRNA abundance of the nuclear factor, erythroid 2 like 2 (NEF2L2) transcription factor and several of its downstream genes known to reduce H2O2. Carnosine prevented the H2O2-mediated activation of p38 MAPK in oxidative stress conditions, whereas it triggered the activation of mTOR under basal conditions. Current results support the protective effect of carnosine against oxidative damage in porcine myoblast cells, an effect that would be mediated through the p38 MAPK intracellular signaling pathway. The activation of the mTOR signaling pathway under basal condition also suggest a role for carnosine in myoblasts proliferation, growth and survival.


Assuntos
Carnosina/metabolismo , Carnosina/farmacologia , Células Satélites de Músculo Esquelético/efeitos dos fármacos , Células Satélites de Músculo Esquelético/metabolismo , Animais , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Perfilação da Expressão Gênica , Glutationa Peroxidase/metabolismo , Peróxido de Hidrogênio/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Fator 2 Relacionado a NF-E2/genética , Estresse Oxidativo/efeitos dos fármacos , Consumo de Oxigênio/efeitos dos fármacos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Sus scrofa , Serina-Treonina Quinases TOR/metabolismo
8.
PLoS One ; 15(9): e0239358, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32970715

RESUMO

There is an extensive literature establishing, validating, and quantifying a wide range of responses of fishes to fasting. Our study complements this work by comparing fed and unfed treatments of hatchery-raised Delta Smelt (Hypomesus transpacificus)-an imperiled fish that is endemic to the San Francisco Estuary and its tributaries in California, USA-across a diverse suite of endpoints over a two-month time series. The experiment was conducted at 15.9°C, and individuals were sampled at 12 time points as starvation became increasingly severe. We found that hepatosomatic index and condition factor were relatively sensitive to starvation, becoming significantly depressed at Day 4 and 7, respectively. Histological analysis of liver showed elevated cytoplasmic inclusion bodies at Day 7, followed by increased glycogen depletion, single cell necrosis, and hydropic vacuolar degeneration at Day 14, 21, and 28, respectively. Of four antioxidants measured, glutathione decreased at Day 4, superoxide dismutase increased at Day 14, catalase increased at Day 56, and glutathione peroxidase was not affected by starvation. The net result was a ~2-fold increase in lipid peroxidation (malondialdehyde) in fasted fish that was highly inconsistent through time. RNA to DNA ratio and triglycerides in muscle were relatively insensitive to starvation, only consistently decreasing with fasting after mortality began increasing in the 'No Feeding' treatment, at Day 21. Together, these results suggest that Delta Smelt mobilize hepatic energy stores far more rapidly than lipids in muscle when subjected to fasting, leading to rapid atrophy of liver and the development of cytoplasmic inclusion bodies-possibly autophagosomes-in hepatocytes.


Assuntos
Osmeriformes/metabolismo , Inanição , Animais , Catalase/metabolismo , Glutationa/metabolismo , Glicogênio/metabolismo , Corpos de Inclusão/metabolismo , Fígado/metabolismo , Fígado/patologia , Malondialdeído/metabolismo , Músculos/metabolismo , Músculos/patologia , Necrose , Osmeriformes/crescimento & desenvolvimento , Superóxido Dismutase/metabolismo , Fatores de Tempo
9.
Ecotoxicol Environ Saf ; 205: 111293, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32949840

RESUMO

Wastewater from printing and dyeing processes often contains aniline and high salinity, which are hazardous to aquatic species. Glycophytic plants cannot survive under high-salinity conditions, whereas halophytes grow well in such an environment. In this study, we investigated the influence of NaCl on the antioxidant level in Suaeda salsa affected by aniline stress. The seedlings showed various growth toxicity effects under different concentrations of aniline. The results showed that the effect of the aniline was more severe for the root growth compared to that for the shoot growth. Aniline exposure significantly increased the total free radicals and ·OH radicals in the plants. Suaeda salsa exposure to aniline caused oxidative stress by altering the superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activity, which resulted in the overproduction of H2O2 and the inducement of lipid peroxidation. Analysis revealed that the malondialdehyde (MDA) content was enhanced after aniline exposure and that the chlorophyll content was significantly decreased. The results showed that aniline induced the production of free radicals and reactive oxygen species (ROS), and changed the antioxidant defense system. This ultimately resulted in oxidative damage in S. salsa; however, it was found that moderate salinity could mitigate the effects. In conclusion, salinity may alleviate the growth inhibition caused by aniline by regulating the antioxidant capacity of S. salsa.


Assuntos
Compostos de Anilina/toxicidade , Antioxidantes/metabolismo , Chenopodiaceae/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Cloreto de Sódio/farmacologia , Poluentes Químicos da Água/toxicidade , Catalase/metabolismo , Chenopodiaceae/enzimologia , Chenopodiaceae/crescimento & desenvolvimento , Clorofila/metabolismo , Espectroscopia de Ressonância de Spin Eletrônica , Peróxido de Hidrogênio/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Malondialdeído/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Salinidade , Plantas Tolerantes a Sal/efeitos dos fármacos , Plantas Tolerantes a Sal/enzimologia , Plantas Tolerantes a Sal/crescimento & desenvolvimento , Plântula/efeitos dos fármacos , Plântula/enzimologia , Plântula/crescimento & desenvolvimento , Superóxido Dismutase/metabolismo
10.
Anticancer Res ; 40(10): 5399-5404, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32988859

RESUMO

BACKGROUND/AIM: The aim of the present study was to investigate whether idarubicin (IDR) induces oxidative DNA damage in the presence of copper (II). MATERIALS AND METHODS: DNA damage was evaluated by pBR322 plasmid DNA cleavage. The formation of oxidative stress markers [O2 •- and 8-hydroxy-2'-deoxyguanosine (8-OHdG)] was analysed. RESULTS: IDR induced DNA damage and O2 •- and 8-OHdG generation in the presence of copper (II). CONCLUSION: IDR induced oxidative DNA damage in the presence of copper (II). Since it has been reported that the concentration of copper in the serum of cancer patients is higher than that in healthy groups, IDR-induced oxidative DNA damage in the presence of copper (II) may play an important role in anticancer therapeutic strategies.


Assuntos
Antraciclinas/farmacologia , Idarubicina/farmacologia , Neoplasias/tratamento farmacológico , Estresse Oxidativo/efeitos dos fármacos , Antraciclinas/química , Cobre/química , Dano ao DNA/efeitos dos fármacos , Humanos , Idarubicina/química , Neoplasias/genética , Neoplasias/patologia , Espécies Reativas de Oxigênio/química , Superóxido Dismutase/genética
11.
Pestic Biochem Physiol ; 170: 104671, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32980069

RESUMO

TEB belongs to the family of triazole fungicides and it is used to protect agricultural crop plants from fungal pathogens. The information regarding its cardiotoxic effects through different pathways particularly by perturbing the oxidative balance and causing damage to the myocardium is still limited. In the present study, oxidative and histopathologic damages caused by TEB in the cardiac tissue of male adult rats, were evaluated. Rats were exposed orally to TEB at 0.9, 9, 27 and 45 mg/kg b.w. for 28 days. Results showed that following TEB treatment malondialdehyde (MDA), protein carbonyl (PC), advanced oxidation protein product (AOPP), antioxidant enzyme activities (GPx and GR) and GSSG levels increased, while GSH levels and thus the GSH/GSSG ratio decreased. Superoxide dismutase (SOD) and catalase (CAT) initially increased at the doses of 0.9, 9 and 27 mg/kg b.w. and then decreased at the dose of 45 mg/kg b.w. Moreover, western blot analysis showed that TEB increased SOD1, CAT and HSP70 protein levels after 24 h. Furthermore, TEB induced various histological changes in the myocardium, including leucocytic infiltration, hemorrhage congestion of cardiac blood vessels and cytoplasmic vacuolization. Therefore, our investigation revealed, that TEB exhibits cardiotoxic effects by changing oxidative balance and damaging the cardiac tissue.


Assuntos
Glutationa , Estresse Oxidativo , Animais , Antioxidantes , Catalase , Glutationa Peroxidase , Masculino , Malondialdeído , Ratos , Ratos Wistar , Superóxido Dismutase , Triazóis/toxicidade
12.
Ecotoxicol Environ Saf ; 203: 110980, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32888623

RESUMO

Pesticides can enter aquatic environments potentially affecting non-target organisms. Unfortunately, the effects of such substances are still poorly understood. This study investigated the effects of the active neonicotinoid substance thiacloprid (TH) and the commercial product Calypso 480 SC (CA) (active compound 40.4% TH) on Mytilus galloprovincialis after short-term exposure to sublethal concentrations. Mussels were tested for seven days to 0, 1, 5 and 10 mg L-1 TH and 0, 10, 50 and 100 mg L-1 CA. For this purpose, several parameters, such as cell viability of haemocytes and digestive cells, biochemical haemolymph features, superoxide dismutase (SOD) and catalase (CAT) enzymatic activity of gills and digestive gland, as well as histology of such tissues were analysed. The sublethal concentrations of both substances lead to abatement or completely stopping the byssal fibres creation. Biochemical analysis of haemolymph showed significant changes (P < 0.01) in electrolytes ions (Cl-, K+, Na+, Ca2+, S-phosphor), lactate dehydrogenase (LDH) enzyme activity and glucose concentration following exposure to both substances. The TH-exposed mussels showed significant imbalance (P < 0.05) in CAT activity in digestive gland and gills. CA caused significant decrease (P < 0.05) in SOD activity in gills and in CAT activity in both tissues. Results of histological analyses showed severe damage in both digestive gland and gills in a time- and concentration-dependent manner. This study provides useful information about the acute toxicity of a neonicotinoid compound and a commercial insecticide on mussels. Nevertheless, considering that neonicotinoids are still widely used and that mussels are very important species for marine environment and human consumption, further researches are needed to better comprehend the potential risk posed by such compounds to aquatic non-target species.


Assuntos
Brânquias/efeitos dos fármacos , Hemolinfa/efeitos dos fármacos , Inseticidas/toxicidade , Mytilus/efeitos dos fármacos , Neonicotinoides/toxicidade , Tiazinas/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Catalase/metabolismo , Sobrevivência Celular , Relação Dose-Resposta a Droga , Brânquias/enzimologia , Hemócitos/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Testes de Toxicidade Aguda
13.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(8): 1103-1111, 2020 Aug 30.
Artigo em Chinês | MEDLINE | ID: mdl-32895185

RESUMO

OBJECTIVE: To evaluate the expression and prognostic value of superoxide dismutase 2 (SOD2) in breast cancer and explore its possible role in the occurrence and progression of breast cancer. METHODS: We performed bioinformatics analysis of the TCGA data for the expression and clinical relevance of SOD2 in patients with breast cancer. Gene enrichment analysis (GSEA) was performed using the KEGG gene set, the protein interaction network was constructed using the STRING database, and the key genes were screened using Cytoscape software. We also collected 60 pairs of primary breast cancer tissue samples and adjacent samples for detecting SOD2 expressions using immunohistochemistry and RT-qPCR and analyzed the correlation of SOD2 expression with the clinicopathological parameters of the patients. RESULTS: The expression of SOD2 was significantly lower in breast cancer tissue than in adjacent tissues with significant correlation with TNM stage and axillary lymph node metastasis (P < 0.05). Kaplan-Meier survival analysis showed that the recurrence-free survival, distant metastasis-free survival (RFS) and post-progressive survival were significantly shorted in patients with high SOD2 expression than in those with low SOD2 expression (P < 0.05). GSEA enrichment analysis indicated that SOD2 played an important role in the JAK-STAT signaling pathway. IL10 and STAT4 were identified as the key genes in the PPI network, and they were both positively correlated with SOD2. In the 60 pairs of clinical samples, SOD2 was highly expressed in breast cancer tissues with close correlation with axillary lymph node metastasis and the expressions of estrogen receptor and androgen receptor (P < 0.05). CONCLUSIONS: The expression of SOD2 in breast cancer is significantly correlated with TNM stage and axillary lymph node metastasis. SOD2 may affect the proliferation, invasion and metastasis of breast cancer cells possibly by regulating IL10 and/or STAT4 to affect the JAK/STAT signaling pathway.


Assuntos
Neoplasias da Mama , Humanos , Metástase Linfática , Prognóstico , Superóxido Dismutase
14.
Anticancer Res ; 40(10): 5701-5706, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32988895

RESUMO

BACKGROUND/AIM: The simultaneous increase of antioxidant CAT (catalase) enzyme and plasma MDA (malonidialdehyde) concentrations versus the numeric rating scale (NRS) pain score following surgery is unknown. Patients and Methods: The study included 114 patients with gallstone disease and 29 patients in the cancer group. RESULTS: Following surgery, the plasma CAT concentrations increased and plasma MDA concentrations decreased in all patients and especially in cancer patients. The linear mixed model time-effect was statistically significant in CAT and MDA (p<0.001 and p=0.02, respectively). In addition, a significant correlation between NRS pain score values and plasma MDA median concentrations in cancer patients was identified (r=0.430, p<0.001). CONCLUSION: The plasma MDA concentrations decreased and CAT concentrations increased significantly in all patients and especially in cancer patients following surgery. The simultaneous increase of antioxidant CAT enzyme with the decrease of plasma MDA may be an important ROS inhibiting mechanism to help patients return to normal antioxidant-oxidant status.


Assuntos
Catalase/sangue , Cálculos Biliares/sangue , Malondialdeído/sangue , Neoplasias/sangue , Dor/sangue , Antioxidantes/metabolismo , Feminino , Cálculos Biliares/patologia , Cálculos Biliares/cirurgia , Glutationa Peroxidase/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/patologia , Neoplasias/cirurgia , Estresse Oxidativo/genética , Dor/patologia , Dor/cirurgia , Medição da Dor , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/sangue
15.
Medicine (Baltimore) ; 99(33): e21242, 2020 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-32871984

RESUMO

Currently there is no effective treatment for vascular dementia (VaD). Pharmacological treatment often lead to severe complications and require drug dosage adjustment. This study investigated the effect of scalp electroacupuncture combined with Memantine in VaD. The safety and antioxidative effect of scalp electroacupuncture were also explored.A retrospective study was conducted and data of inpatients of Linyi Central Hospital with VaD between June 2017 and May 2018 were collected and sorted. The patients were divided into scalp electroacupuncture-medication (A), scalp electroacupuncture (B) and medication (control) (C) groups, in which Memantine was prescribed as medication. Cognitive function, activities of daily living and quality of life assessed by Montreal Cognitive Assessment (MoCA), Barthel index and dementia quality of life questionnaire; the contents of superoxide dismutase, lipid peroxide and nitric oxide in blood samples; and adverse reaction were compared.Data from a total of 150 patients were collected (Group A, n = 55; Group B, n = 50; Group C, n = 45). The post-treatment/follow-up Montreal Cognitive Assessment, Barthel index and dementia quality of life questionnaire scores were significantly improved in all groups compared to pre-treatment (groups A and B, P<.01; group C, P<.05). The improvements were significant for groups A vs C, B vs C (P<0.01, both), and group A vs B (P<.05). The post-treatment/follow-up levels of lipid peroxide and nitric oxide decreased significantly while superoxide dismutase increased significantly in groups A and B compared to pre-treatment (P<.01, both). The differences were significant for groups A vs C, and B vs C (P < .01, both), but not significant between groups A and B (P > .05). There were no significant adverse events occurred during the study and follow-up.In combined treatment, scalp electroacupuncture works in parallel with Memantine and significantly increase the therapeutic effect in VaD with no significant adverse events. Scalp electroacupuncture may have the potential to serve as an option or alternative treatment for VaD. Scalp electroacupuncture may alleviate VaD symptoms through its antioxidative mechanism.


Assuntos
Demência Vascular/terapia , Eletroacupuntura , Memantina/uso terapêutico , Nootrópicos/uso terapêutico , Atividades Cotidianas , Idoso , Biomarcadores/sangue , Cognição/efeitos dos fármacos , Terapia Combinada , Demência Vascular/sangue , Eletroacupuntura/efeitos adversos , Eletroacupuntura/métodos , Feminino , Seguimentos , Humanos , Peróxidos Lipídicos/sangue , Masculino , Memantina/efeitos adversos , Óxido Nítrico/sangue , Nootrópicos/efeitos adversos , Qualidade de Vida , Estudos Retrospectivos , Couro Cabeludo , Superóxido Dismutase/sangue , Resultado do Tratamento
16.
An Acad Bras Cienc ; 92(suppl 2): e20181147, 2020 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-32901676

RESUMO

Organic selenium, tellurium and sulfur compounds have been studied due to their pharmacological properties. For instance, the ß-aryl-chalcogenium azide compounds have demonstrated antitumoral action in vitro. However, yet no pharmacological actions of this class of compounds were determined in vivo. Caenorhabditis elegans is a nematode that presents innumerable advantages in relation to mammalian models, such as having a small and transparent body, which allows the visualization of its internal anatomy, besides short life and low cost. Based on that, the aim of this work was to investigate the pharmacological and toxicological properties of ß-aryl-chalcogenium azide compounds in C. elegans. As well, to evaluate the capacity of organochalcogenium compounds to repair oxidative damage induced by hydrogen peroxide and the possible mechanism of action of these compounds using CF1553 transgenic strain with superoxide dismutase (SOD-3) tagged with GFP. Our results showed that ß-aryl-chalcogenium azide have low toxicity in wild-type worms and the pre-treatment protected against the damage induced by hydrogen peroxide at higher tested concentration. Associated with this, we observed that this protection is due in part to the increased expression of the antioxidant enzyme SOD-3. In conclusion, ß-aryl-chalcogenium azide compounds caused low toxicity and induced stress-resistance by modulating SOD-3 expression in C. elegans.


Assuntos
Caenorhabditis elegans , Animais , Antioxidantes , Azidas , Superóxido Dismutase
17.
Chem Biol Interact ; 330: 109245, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32866465

RESUMO

The calcineurin inhibitor, cyclosporin A (CsA) is one of the most common immunosuppressive agents used in organ transplantation. However, its clinical use is often limited by several unwanted effects including nephrotoxicity and hepatotoxicity. By using immunohistochemical and ELISA techniques, it was found that CsA administration causes a rapid activation of a disintegrin and metalloproteases-17 (ADAM-17), epidermal growth factor receptor (EGFR) and subsequent ERK1/2 phosphorylation in the liver and kidney of albino mice. Furthermore, this study presents mechanistic relevance of this signaling cascade involving reactive oxygen species (ROS)-mediated ADAM-17/EGFR/ERK1/2 activation as indicated by a clear reduction in ADAM-17 and EGFR activities as well as ERK1/2 phosphorylation when the animals pretreated with Polyethylene glycol-superoxide dismutase (PEG-SOD) before CsA administration. Collectively, our findings demonstrate that CsA has the ability to activate ADAM-17-mediated EGFR/ERK1/2 phosphorylation in the liver and kidney of albino mice in ROS-dependent manner. Finally, these data may support the concept of using antioxidant therapy as a valuable approach for the prevention of CsA-induced nephrotoxicity and hepatotoxicity.


Assuntos
Ciclosporina/toxicidade , Rim/metabolismo , Fígado/metabolismo , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Polietilenoglicóis/farmacologia , Superóxido Dismutase/farmacologia , Proteína ADAM17/metabolismo , Animais , Ciclosporina/farmacologia , Interações Medicamentosas , Receptores ErbB/metabolismo , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Camundongos , Fosforilação/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo
18.
Stroke ; 51(10): 3142-3146, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32819193

RESUMO

BACKGROUND AND PURPOSE: There is an urgent need to develop adjunct therapies that can be added onto reperfusion for acute ischemic stroke. Recently, mitochondrial transplantation has emerged as a promising therapeutic approach for boosting brain tissue protection. In this proof-of-concept study, we investigate the feasibility of using placenta as a source for mitochondrial transplantation in a mouse model of transient focal cerebral ischemia-reperfusion. METHODS: Mitochondria-enriched fractions were isolated from cryopreserved mouse placenta. Mitochondrial purity and JC1 membrane potentials were assessed by flow cytometry. Adenosine triphosphate and mitochondrial proteins were measured by luminescence intensity and western blot, respectively. Therapeutic efficacy of mitochondrial fractions was assessed in a mouse model of transient focal cerebral ischemia-reperfusion. RESULTS: Flow cytometry analysis demonstrated that about 87% of placental mitochondria were viable and maintained JC1 membrane potentials after isolation. Placental mitochondrial fractions contained adenosine triphosphate equivalent to mitochondrial fractions isolated from skeletal muscle and brown fat tissue. Normalized mitochondrial antioxidant enzymes (glutathione reductase, MnSOD [manganese superoxide dismutase]) and HSP70 (heat shock protein 70) were highly preserved in placental mitochondrial fractions. Treatment with placental mitochondrial fractions immediately after reperfusion significantly decreased infarction after focal cerebral ischemia in mice. CONCLUSIONS: Cryopreserved placenta can be a feasible source for viable mitochondrial isolation. Transplantation with placental mitochondria may amplify beneficial effects of reperfusion in stroke.


Assuntos
Mitocôndrias/transplante , Placenta/transplante , Traumatismo por Reperfusão/terapia , Animais , Feminino , Citometria de Fluxo , Glutationa Redutase/metabolismo , Proteínas de Choque Térmico HSP70/metabolismo , Potencial da Membrana Mitocondrial/fisiologia , Camundongos , Placenta/metabolismo , Gravidez , Traumatismo por Reperfusão/metabolismo , Superóxido Dismutase/metabolismo
19.
Sci Total Environ ; 748: 141342, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32818888

RESUMO

S-metolachlor (S-ME) is a widely used chiral herbicide that can cause potential ecological risks via long-term usage. In this work, we chose a model plant, wheat, as the test material to determine the effects of applying 10 mg/kg S-ME to soil on its fresh weight, chlorophyll and malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity and the diversity and structural composition of the phyllosphere microorganisms after 7 and 14 days of exposure. Our work showed that this concentration of residual S-ME in soil only slightly decreased plant biomass and had little effect on lipid peroxidation, the antioxidant enzyme system and chlorophyll content. Interestingly, although the test concentration of S-ME did not exert strong inhibitory effects on the physiological activities of wheat, it decreased the diversity of phyllosphere microbial communities and changed their structure, indicating that microorganisms were more sensitive stress indicators. S-ME reduced the colonization by some beneficial bacteria related to plant nitrogen fixation among the phyllosphere microorganisms, which influenced the growth and yield of wheat because these bacteria contribute to plant fitness. In addition, S-ME affected the association between the host and the composition of the phyllosphere microbial communities under different growth conditions. Our work provides insights into the ecological implications of the effects of herbicides on the phyllosphere microbiome.


Assuntos
Microbiota , Triticum , Acetamidas , Clorofila , Solo , Superóxido Dismutase
20.
Chemosphere ; 261: 127585, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32739687

RESUMO

The utilization of tungsten in traffic, smelting, mining, and other industrial applications allows its' accumulation in the environmental ecosystems. The present study included using a soluble form of tungsten (tungstate) at different levels (0, 1, 5, 10, 50, and 100 mg L-1) as a water contaminant. The germinating seeds experienced tungstate at 1-50 mg L-1 exhibited stimulation of seedling dry and fresh matter stress tolerance indices, whereas retardation of these traits at the level of 100 mg L-1 was manifested. The stimulation of seedling growth at the levels of 1-50 mg L-1 was associated with the regulation of reactive oxygen status, higher stability of cell membrane, and elevated level of antioxidative responses. Regarding the oxidative stress of the seedlings exposed to tungstate contaminated water, only the concentration of 100 mg L-1 induced accumulation of hydrogen peroxide, superoxide anion, and hydroxyl radical with apparent membrane deteriorations in terms of lipid peroxidation. Furthermore, reductions of phytochelatins, reduced glutathione, ascorbate, ascorbate peroxidase, glutathione peroxidase, as well as glutathione-S-transferase were the main symptoms of tungstate phytotoxicity at the same level. The accumulation of lignin, ionic peroxidase, soluble peroxidase, and lignin-related enzymes (phenylalanine ammonia-lyase and polyphenol oxidase) were the striking reasons for restricting seedlings growth at noxious tungstate level. The results could suggest that the elevated levels of defense systems, at least in part, were accountable for raising broccoli resistance against tungstate stress at low doses. Furthermore, these plants can grow in tungsten-polluted areas by modifying their physiological processes. However, this study shed the light to the eco-toxicity of tungstate and imparts evidence for the need to establishing environmental risk management of tungstate accumulation.


Assuntos
Antioxidantes/metabolismo , Brassica/fisiologia , Compostos de Tungstênio/toxicidade , Poluentes Químicos da Água/toxicidade , Ascorbato Peroxidases/metabolismo , Ácido Ascórbico/metabolismo , Brassica/metabolismo , Catalase/metabolismo , Ecossistema , Germinação , Glutationa/metabolismo , Oxirredução , Estresse Oxidativo , Plântula/metabolismo , Sementes/metabolismo , Superóxido Dismutase/metabolismo
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