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1.
Clin Ter ; 171(6): e490-e500, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33151247

RESUMO

BACKGROUND: Post Mortem Computed Tomography (PMCT) is being increasingly implemented in forensic field and could be an adjuvant to classic autopsies. In this study we evaluated the feasibility of complementation of conventional autopsy in trauma victims with PMCT. MATERIALS AND METHODS: A total of 21 subjects, who had sustained various types of blunt high-energy trauma, were selected from the casuistry of the Section of Legal Medicine at University of Pisa: before autopsy, a PMCT examination (Toshiba Aquilion 16 CT scanner) was performed, and after the acquisition of the raw images, MPR and VR reconstructions were performed with dedicated software. RESULTS: PMCT is more sensitive than conventional autopsy in detecting skeletal injuries, whilst autopsy constitutes the method of choice for the detection of thoracic and abdominal visceral injuries. CONCLUSIONS: PMCT should be considered a useful tool in addition to conventional autopsy in evaluating trauma victims: it detects further bone fractures in body parts difficult to investigate during autopsy (i.e. posterior regions), facilitating the pathologist in the reconstruction of events and in determining the cause of death.


Assuntos
Autopsia/métodos , Tomografia Computadorizada por Raios X/métodos , Ferimentos não Penetrantes/diagnóstico por imagem , Traumatismos Abdominais/diagnóstico por imagem , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Osso e Ossos/diagnóstico por imagem , Feminino , Medicina Legal , Patologia Legal/métodos , Técnicas Histológicas , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
2.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 248-251, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33017975

RESUMO

Accurate and reliable detecting of driving fatigue using Electroencephalography (EEG) signals is a method to reduce traffic accidents. So far, it is natural to cut the part of operating the steering wheel data away for achieving the relatively high accuracy in detecting driving fatigue using EEG data. However, the data segment during operating the steering wheel also contains valuable information. Moreover, operating the steering wheel is a common practice during actual driving. In this study, we utilize the part of data operating the steering wheel to detecting fatigue. The feature used is the spectral band power calculates from the data. For each experiment and each experimental participant, the data and features are divided into sessions and subjects. Using the divided features, this work performs cross-session and cross-subject verification and comparison on the two classification methods of logistic regression and multi-layer perceptron. To compare the effect, the experiment is conducted on the data both operating the steering wheel and not operating the steering wheel. The result shows that the bias between the average accuracy of two types of data is only 2.27%, and the effect of using multi-layer perceptron is 10.37% better than using logistic regression. This proves that the data segment during operating the steering wheel also contains valid information and can be used for driving fatigue detection.


Assuntos
Condução de Veículo , Acidentes de Trânsito/prevenção & controle , Eletroencefalografia , Técnicas Histológicas , Humanos , Equipamentos de Proteção
3.
Rev. esp. patol ; 53(3): 188-192, jul.-sept. 2020. ilus
Artigo em Inglês | IBECS | ID: ibc-192406

RESUMO

The new coronavirus SARS-CoV-2, first identified in Wuhan, China in December, 2019, can cause Severe Acute Respiratory Syndrome (SARS) with massive alveolar damage and progressive respiratory failure. We present the relevant autopsy findings of the first patient known to have died from COVID19 pneumonia in Spain, carried out on the 14th of February, 2020, in our hospital (Hospital Arnau de Vilanova-Lliria, Valencia). Histological examination revealed changes typical of diffuse alveolar damage (DAD) in both the exudative and proliferative phase of acute lung injury. Intra-alveolar multinucleated giant cells, smudge cells and vascular thrombosis were present. The diagnosis was confirmed by reverse real-time PCR assay on a throat swab sample taken during the patient's admission. The positive result was reported fifteen days subsequent to autopsy


El nuevo coronavirus SARS-CoV-2, identificado inicialmente en China en diciembre de 2019 puede cursar con un Síndrome Respiratorio Agudo Severo (SARS) con daño alveolar masivo y fracaso respiratorio progresivo. Presentamos los hallazgos más relevantes encontrados en la autopsia clínica efectuada en nuestro hospital (Hospital Arnau de Vilanova-Lliria de Valencia) a fecha de 14 de febrero de 2020, al primer paciente fallecido conocido en España por neumonía COVID-19. A nivel pulmonar, la autopsia revela cambios típicos de daño alveolar difuso (DAD) en fase exudativa y fase proliferativa. Se observan células multinucleadas gigantes, células tipo smudge intraalveolares y trombosis vasculares. El diagnóstico microbiológico confirmativo mediante PCR se realizó 15 días después de la autopsia sobre la muestra faríngea del enfermo tomada durante su ingreso


Assuntos
Humanos , Masculino , Idoso , Autopsia/métodos , Infecções por Coronavirus/mortalidade , Síndrome Respiratória Aguda Grave/mortalidade , Vírus da SARS/isolamento & purificação , Técnicas Histológicas/métodos , Alvéolos Pulmonares/patologia , Espanha/epidemiologia , Causas de Morte , Pandemias
4.
Rev. esp. patol ; 53(3): 182-187, jul.-sept. 2020. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-192407

RESUMO

We describe the implementation of a COVID-19 Autopsy Programme in our Hospital, report the main findings from the first autopsy of the programme and briefly review the reports of lung pathology of these patients


En este artículo presentamos el proceso de implementación de un Programa de Autopsias COVID-19 en nuestro hospital, presentamos los principales hallagos de la primera autopsia realizada y revisamos brevemente la patología pulmonar publicada previamente en estos pacientes


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Embolia Pulmonar/complicações , Autopsia/estatística & dados numéricos , Causas de Morte , Infecções por Coronavirus/patologia , Alvéolos Pulmonares/patologia , Síndrome Respiratória Aguda Grave/complicações , Vírus da SARS/isolamento & purificação , Apneia Obstrutiva do Sono/complicações , Hipertensão/complicações , Reação em Cadeia da Polimerase/métodos , Esteroides/uso terapêutico , Pandemias , Técnicas Histológicas/métodos , Espanha/epidemiologia
5.
Nat Commun ; 11(1): 4686, 2020 09 17.
Artigo em Inglês | MEDLINE | ID: mdl-32943633

RESUMO

Electrophysiology provides a direct readout of neuronal activity at a temporal precision only limited by the sampling rate. However, interrogating deep brain structures, implanting multiple targets or aiming at unusual angles still poses significant challenges for operators, and errors are only discovered by post-hoc histological reconstruction. Here, we propose a method combining the high-resolution information about bone landmarks provided by micro-CT scanning with the soft tissue contrast of the MRI, which allowed us to precisely localize electrodes and optic fibers in mice in vivo. This enables arbitrating the success of implantation directly after surgery with a precision comparable to gold standard histology. Adjustment of the recording depth with micro-drives or early termination of unsuccessful experiments saves many working hours, and fast 3-dimensional feedback helps surgeons avoid systematic errors. Increased aiming precision enables more precise targeting of small or deep brain nuclei and multiple targeting of specific cortical or hippocampal layers.


Assuntos
Encéfalo/diagnóstico por imagem , Eletrodos Implantados , Processamento de Imagem Assistida por Computador/métodos , Fibras Ópticas , Microtomografia por Raio-X/métodos , Animais , Comportamento Animal , Encéfalo/patologia , Mapeamento Encefálico , Hipocampo/diagnóstico por imagem , Hipocampo/patologia , Hipocampo/cirurgia , Técnicas Histológicas/métodos , Imagem por Ressonância Magnética/métodos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Modelos Animais , Silício , Técnicas Estereotáxicas
6.
PLoS One ; 15(9): e0239562, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32966330

RESUMO

Reproducible and unbiased methods to quantify alveolar structure are important for research on many lung diseases. However, manually estimating alveolar structure through stereology is time consuming and inter-observer variability is high. The objective of this work was to develop and validate a fast, reproducible and accurate (semi-)automatic alternative. A FIJI-macro was designed that automatically segments lung images to binary masks, and counts the number of test points falling on tissue and the number of intersections of the air-tissue interface with a set of test lines. Manual selection remains necessary for the recognition of non-parenchymal tissue and alveolar exudates. Volume density of alveolar septa ([Formula: see text]) and mean linear intercept of the airspaces (Lm) as measured by the macro were compared to theoretical values for 11 artificial test images and to manually counted values for 17 lungs slides using linear regression and Bland-Altman plots. Inter-observer agreement between 3 observers, measuring 8 lungs both manually and automatically, was assessed using intraclass correlation coefficients (ICC). [Formula: see text] and Lm measured by the macro closely approached theoretical values for artificial test images (R2 of 0.9750 and 0.9573 and bias of 0.34% and 8.7%). The macro data in lungs were slightly higher for [Formula: see text] and slightly lower for Lm in comparison to manually counted values (R2 of 0.8262 and 0.8288 and bias of -6.0% and 12.1%). Visually, semi-automatic segmentation was accurate. Most importantly, manually counted [Formula: see text] and Lm had only moderate to good inter-observer agreement (ICC 0.859 and 0.643), but agreements were excellent for semi-automatically counted values (ICC 0.956 and 0.900). This semi-automatic method provides accurate and highly reproducible alveolar morphometry results. Future efforts should focus on refining methods for automatic detection of non-parenchymal tissue or exudates, and for assessment of lung structure on 3D reconstructions of lungs scanned with microCT.


Assuntos
Displasia Broncopulmonar/patologia , Interpretação de Imagem Assistida por Computador/métodos , Alvéolos Pulmonares/patologia , Animais , Displasia Broncopulmonar/diagnóstico por imagem , Modelos Animais de Doenças , Feminino , Técnicas Histológicas/estatística & dados numéricos , Variações Dependentes do Observador , Gravidez , Alvéolos Pulmonares/diagnóstico por imagem , Coelhos , Interpretação de Imagem Radiográfica Assistida por Computador/métodos , Microtomografia por Raio-X/estatística & dados numéricos
7.
Wiad Lek ; 73(4): 789-791, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32731718

RESUMO

OBJECTIVE: The aim of the study to evaluate the peculiarities of the aortic wall structure at the place of coarctation. PATIENTS AND METHODS: Materials and methods: Studying of the aortic sections removed during operative correction at the place of constriction. 10 children at the age between 1 to 6 months were undergone the operation. Intraoperative aortic biopsy specimens were observed in 10% neutral formalin. Histologic sections were prepared in a conventional way followed by staining them with hematoxylin-eosin. RESULTS: Results: Histological examination in the areas of constriction revealed that the endothelium in all the preparations had poor expressiveness. The most significant changes were recorded in the middle layer of the aorta in the form of reduced development of elastic fibers, their fragmentation and chaotic arrangement. Angiomatosis with the formation of thin-layer small vessels by capillary type was found out. In all the preparations, areas of emptying of cells and fibers of the middle cover with the formation of cystic structures were revealed. CONCLUSION: Conclusions: The histological examination has revealed changes in the structure of the aorta wall, which may indicate the systemic nature of the lesion and make it possible to consider coarctation of the aorta to be a manifestation of systemic vasculopathy. The above-mentioned facts determine the need for a more detailed examination of children with the specified pathology at different stages of observation.


Assuntos
Coartação Aórtica , Aorta , Constrição Patológica , Técnicas Histológicas , Humanos , Lactente , Coloração e Rotulagem
8.
Adv Exp Med Biol ; 1262: 19-38, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32613578

RESUMO

Embryology and histology are subjects that are viewed as particularly challenging by students in higher education. This negative perception is the result of many factors such as restricted access to lab facilities, lack of allocated time to these labs, and the complexity of the subject itself. One main factor that influences this viewpoint is the difficulty of grasping 3D orientation of sectioned tissues, especially regarding embryology. Attempts have been made previously to create alternative teaching methods to help alleviate these issues, but few have explored 3D visualisation. We aimed to address these issues by creating 3D embryological reconstructions from serial histology sections of a sheep embryo. These were deployed in a mobile application that allowed the user to explore the original sections in sequence, alongside the counterpart 3D model. The application was tested against a currently available eHistology programme on a cohort of life sciences graduates (n = 14) through qualitative surveys and quantitative testing through labelling and orientation-based tests. The results suggest that using a 3D modality such as the one described here significantly improves student comprehension of orientation of slides compared to current methods (p = 0.042). Furthermore, the developed application was deemed more interesting, useful, and usable than current eHistology tools (p < 0.05). Modalities such as that developed here could therefore provide a more effective approach to learning these challenging subjects potentially increasing student engagement with embryology and histology.


Assuntos
Compreensão , Instrução por Computador , Embrião de Mamíferos , Embriologia , Animais , Instrução por Computador/métodos , Instrução por Computador/normas , Embriologia/educação , Técnicas Histológicas , Humanos , Imageamento Tridimensional , Aprendizagem , Ovinos
9.
Rev. ADM ; 77(3): 162-167, mayo-jun. 2020. ilus
Artigo em Espanhol | LILACS | ID: biblio-1128895

RESUMO

Introducción: El síndrome de Gorlin-Goltz o síndrome de carcinoma de nevo basocelular es un desorden hereditario autosómico dominante que predispone principalmente a la proliferación de múltiples carcinomas basocelulares, queratoquistes odontogénicos y defectos del desarrollo, causados por la mutación del gen Patched localizado en el cromosoma 9. Presentación del caso: Se reporta un paciente con características de este síndrome, en la clínica de COMF de la UNAM. El diagnóstico fue basado en los estudios clínicos, imagenológicos y moleculares. Conclusiones: El conocimiento de esta enfermedad puede orientarnos a la sospecha diagnóstica de lesión quística o premaligna en forma oportuna, lo que permite prevenir complicaciones y brindar un tratamiento integral para así mejorar la calidad de vida de este tipo de pacientes (AU)


Introduction: Gorlin-Goltz syndrome or cell-based nevus carcinoma syndrome is an autosomal dominant inherited disorder that predisposes mainly to the proliferation of multiple basal cell carcinomas, maxillary keratocysts and developmental defects, caused by the mutation of the Patched gene located on chromosome 9. Case presentation: A patient with specific characteristics compatible with this syndrome was reported in the COMF Department of the UNAM. The diagnosis was based on clinical studies, radiology and genetic studies. Conclusions: Knowledge of this problem can guide us to the diagnostic suspicion in a timely manner, thus preventing complications, and to provide an improved integral treatment of the quality of life of this type of patients (AU)


Assuntos
Humanos , Masculino , Criança , Carcinoma Basocelular , Síndrome do Nevo Basocelular , Cistos Odontogênicos/cirurgia , Manifestações Bucais , Biópsia , Técnicas Histológicas , Patologia Molecular , Receptor Patched-1 , México
10.
Recent Results Cancer Res ; 216: 795-812, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32594407

RESUMO

In this chapter, we will introduce and review molecular-sensitive imaging techniques, which close the gap between ex vivo and in vivo analysis. In detail, we will introduce spontaneous Raman spectral imaging, coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS), second-harmonic generation (SHG) and third-harmonic generation (THG), two-photon excited fluorescence (TPEF), and fluorescence lifetime imaging (FLIM). After reviewing these imaging techniques, we shortly introduce chemometric methods and machine learning techniques, which are needed to use these imaging techniques in diagnostic applications.


Assuntos
Técnicas Histológicas , Imagem Molecular , Análise Espectral Raman , Humanos
11.
Sci Data ; 7(1): 185, 2020 06 19.
Artigo em Inglês | MEDLINE | ID: mdl-32561748

RESUMO

The distribution and appearance of nuclei are essential markers for the diagnosis and study of cancer. Despite the importance of nuclear morphology, there is a lack of large scale, accurate, publicly accessible nucleus segmentation data. To address this, we developed an analysis pipeline that segments nuclei in whole slide tissue images from multiple cancer types with a quality control process. We have generated nucleus segmentation results in 5,060 Whole Slide Tissue images from 10 cancer types in The Cancer Genome Atlas. One key component of our work is that we carried out a multi-level quality control process (WSI-level and image patch-level), to evaluate the quality of our segmentation results. The image patch-level quality control used manual segmentation ground truth data from 1,356 sampled image patches. The datasets we publish in this work consist of roughly 5 billion quality controlled nuclei from more than 5,060 TCGA WSIs from 10 different TCGA cancer types and 1,356 manually segmented TCGA image patches from the same 10 cancer types plus additional 4 cancer types.


Assuntos
Núcleo Celular/patologia , Técnicas Histológicas , Processamento de Imagem Assistida por Computador , Neoplasias/diagnóstico por imagem , Neoplasias/patologia , Amarelo de Eosina-(YS) , Hematoxilina , Humanos
12.
PLoS One ; 15(6): e0234736, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32574166

RESUMO

Imaging techniques have proved to be crucial for diagnosis in reptile species. The topography of the internal organs of bearded dragons has been described in recent studies as meeting the small animal practitioners´ demand for knowledge concerning their anatomy. However, the nomenclature in the respective literature is not uniform, which could lead to misunderstandings concerning the respective and/or affected parts of the alimentary canal. Therefore, the aim of this study was to provide clear information on anatomy and histology of the alimentary canal of bearded dragons including supplying blood vessels. For the dissection of the alimentary canal, 11 Inland Bearded Dragons (Pogona vitticeps) were used (five males, six females), which had been euthanised for clinical reasons other than those concerning the digestive tract or had died spontaneously. The supplying arteries were demonstrated by injecting red latex into the aorta, while the intestinal veins were filled with blue latex via the portal vein. Microscopic examination was carried out on specimens of seven additional bearded dragons using routine histologic procedures. Macroscopically, the sections of the alimentary canal from oral to aboral were distinguished into oesophagus, stomach, small intestine, colic ampulla, colic isthmus, rectum and cloaca. Differentiation of the duodenum, jejunum and ileum was only possible when considering the bile duct, the vasculature and the histology of the organ wall. Arteries supplying the oesophagus and the final straight part of the large intestine originated from the aorta in a segmental manner. Between these, three unpaired arteries arose from the aorta. Their branches supplied stomach and intestine excluding its last part. Based on the findings of the present study, a nomenclature for the different parts of the alimentary canal and the supplying blood vessels of bearded dragons is suggested which is well understandable for veterinary practitioners and is based on zoological knowledge of reptiles.


Assuntos
Vasos Sanguíneos/anatomia & histologia , Trato Gastrointestinal/anatomia & histologia , Trato Gastrointestinal/citologia , Lagartos/anatomia & histologia , Animais , Vasos Sanguíneos/fisiologia , Feminino , Técnicas Histológicas , Masculino , Neovascularização Fisiológica
13.
Sci Rep ; 10(1): 10077, 2020 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-32572077

RESUMO

To establish a histopathological scoring system for changes in subchondral bone in murine models of knee osteoarthritis (OA), three key parameters, subchondral bone plate (Subcho.BP) consisting of the combination of Subcho.BP.thickness (Subcho.BP.Th) and angiogenesis, bone volume (BV/TV) and osteophytes, were selected. The new grading system was tested in two mouse OA models, (1) senescence accelerated mouse (SAM)-prone 8 (SAMP8) as spontaneous OA model with SAM-resistant 1 (SAMR1) as control; (2) destabilization of the medial meniscus in C57BL/6 mice as surgical OA model. Results of the spontaneous OA model showed that Subcho.BP.Th was significantly wider, angiogenesis was greater, and BV/TV was higher in SAMP8 than SAMR1. Notably, subchondral bone score was dramatically higher in SAMP8 at 6 weeks than SAMR1, while OARSI cartilage scores became higher only at 14 weeks. In the surgical OA model, the results were similar to the spontaneous OA model, but osteophytes appeared earlier. There were strong correlations both in Subcho.BP.Th and BV/TV between this scoring system and µCT (r = 0.89, 0.84, respectively). Inter-rater reliabilities for each parameter using this system were more than 0.943. We conclude that this new histopathological scoring system is readily applicable for evaluating the early changes in aging and OA-affected murine subchondral bone.


Assuntos
Envelhecimento/patologia , Osso e Ossos/patologia , Osteoartrite/patologia , Animais , Cartilagem Articular/patologia , Modelos Animais de Doenças , Técnicas Histológicas/métodos , Técnicas Histológicas/normas , Masculino , Meniscos Tibiais/patologia , Camundongos , Camundongos Endogâmicos C57BL , Osteoartrite do Joelho/patologia , Osteófito/patologia , Tíbia/patologia
17.
Sci Rep ; 10(1): 9911, 2020 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-32555410

RESUMO

Automatically detecting and grading cancerous regions on radical prostatectomy (RP) sections facilitates graphical and quantitative pathology reporting, potentially benefitting post-surgery prognosis, recurrence prediction, and treatment planning after RP. Promising results for detecting and grading prostate cancer on digital histopathology images have been reported using machine learning techniques. However, the importance and applicability of those methods have not been fully investigated. We computed three-class tissue component maps (TCMs) from the images, where each pixel was labeled as nuclei, lumina, or other. We applied seven different machine learning approaches: three non-deep learning classifiers with features extracted from TCMs, and four deep learning, using transfer learning with the 1) TCMs, 2) nuclei maps, 3) lumina maps, and 4) raw images for cancer detection and grading on whole-mount RP tissue sections. We performed leave-one-patient-out cross-validation against expert annotations using 286 whole-slide images from 68 patients. For both cancer detection and grading, transfer learning using TCMs performed best. Transfer learning using nuclei maps yielded slightly inferior overall performance, but the best performance for classifying higher-grade cancer. This suggests that 3-class TCMs provide the major cues for cancer detection and grading primarily using nucleus features, which are the most important information for identifying higher-grade cancer.


Assuntos
Algoritmos , Diagnóstico por Computador/métodos , Aprendizado de Máquina , Recidiva Local de Neoplasia/diagnóstico , Prostatectomia/métodos , Neoplasias da Próstata/classificação , Neoplasias da Próstata/patologia , Técnicas Histológicas , Humanos , Masculino , Gradação de Tumores , Recidiva Local de Neoplasia/cirurgia , Neoplasias da Próstata/cirurgia
18.
PLoS One ; 15(5): e0231602, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32469877

RESUMO

Reversible Data Hiding (RDH) techniques have gained popularity over the last two decades, where data is embedded in an image in such a way that the original image can be restored. Earlier works on RDH was based on the Image Histogram Modification that uses the peak point to embed data in the image. More recent works focus on the Difference Image Histogram Modification that exploits the fact that the neighbouring pixels of an image are highly correlated and therefore the difference of image makes more space to embed large amount of data. In this paper we propose a framework to increase the embedding capacity of reversible data hiding techniques that use a difference of image to embed data. The main idea is that, instead of taking the difference of the neighboring pixels, we rearrange the columns (or rows) of the image in a way that enhances the smooth regions of an image. Any difference based technique to embed data can then be used in the transformed image. The proposed method is applied on different types of images including textures, patterns and publicly available images. Experimental results demonstrate that the proposed method not only increases the message embedding capacity of a given image by more than 50% but also the visual quality of the marked image containing the message is more than the visual quality obtained by existing state-of-the-art reversible data hiding technique. The proposed technique is also verified by Pixel Difference Histogram (PDH) Stegoanalysis and results demonstrate that marked images generated by proposed method is undetectable by PDH analysis.


Assuntos
Algoritmos , Segurança Computacional/normas , Simulação por Computador , Bases de Dados Factuais , Técnicas Histológicas/métodos , Processamento de Imagem Assistida por Computador/métodos , Humanos , Registros
19.
J Ayub Med Coll Abbottabad ; 32(1): 83-86, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32468762

RESUMO

BACKGROUND: The aim of this study was to compare the diagnostic utility of fine needle aspiration cytology (FNAC) in breast lesion with gold standard of histopathological diagnosis. METHODS: This descriptive cross-sectional study was conducted in Pathology department of Bannu Medical College in collaboration with Surgery Department of Khalifa Gul Nawaz Teaching Hospital Bannu as well as other private surgical hospitals of the territory. A total of 88 FNAC of palpable breast lesions were performed and compared with their corresponding histopathological reports. Patients age, location of lesion in breast, and other necessary findings were recorded on predesigned proforma, FNAC performed by using 10 ml syringes. Slides prepared and processed for cytological staining and reported by cytopathologist. Corresponding biopsies were processed for histopathological examination and diagnosis. All female patients of any age with breast lesions were included, and those breast lesions for which no histopathological report was available were excluded. Statistical Package for Social Sciences (SPSS) version 20 was used for calculation of frequencies with percentages and mean with standard deviation. Also, specificity, sensitivity, diagnostic accuracy, positive predictive value and negative predictive value were calculated by using formulas. RESULTS: In this study the mean age was 34.44±21.57 years and age range was from 16 to 80 years. The most common age group was 26-35 years followed by 36-45 years. In this study sensitivity was (83.33%), specificity was (100 %), positive predictive value was (100 %), negative predictive value was (99.27%) and diagnostic accuracy was 99.30%. CONCLUSIONS: FNAC breast is an easily approachable, less invasive, cheap, rapid and almost accurate diagnostic tool in the diagnosis of palpable as well as non palpable breast lesions.


Assuntos
Biópsia por Agulha Fina/estatística & dados numéricos , Neoplasias da Mama , Mama/patologia , Técnicas Histológicas/estatística & dados numéricos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/patologia , Estudos Transversais , Feminino , Humanos , Pessoa de Meia-Idade , Paquistão , Valor Preditivo dos Testes , Adulto Jovem
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