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1.
J Int Med Res ; 49(2): 300060520982687, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33527860

RESUMO

OBJECTIVE: To evaluate the efficacy of rapid on-site cytological evaluation (ROSE) in determining specimen adequacy and diagnostic accuracy in the interventional diagnosis of lung lesions. METHODS: This retrospective study included 127 consecutive cases of lung lesions, which were sampled by bronchoscopy or transthoracic fine needle aspiration, and diagnosed on ROSE followed by histopathology. ROSE was performed by a trained pulmonologist and the diagnosis of ROSE was compared with the final diagnosis. RESULTS: The sensitivity of ROSE in determining adequacy of specimens was 97.5% and specificity in determining inadequacy was 85.7%. The diagnostic efficacy of ROSE for assessing malignancy (sensitivity of 94.5% and specificity of 100%) and non-malignancy (sensitivity of 97.8% and specificity of 100%) was excellent. The sensitivity of ROSE for diagnosing small cell carcinoma (100%) was highest, followed by adenocarcinoma (89.2%) and squamous cell carcinoma (75.0%). Performance of ROSE by a trained pulmonologist also determined tuberculosis with a high diagnostic sensitivity (83.3%) and specificity (100%). CONCLUSIONS: A trained pulmonologist can reliably carry out ROSE to ensure the adequacy of the sample, distinguish between malignancy and non-malignancy, and make a preliminary diagnosis in a large number of cases.


Assuntos
Adenocarcinoma/diagnóstico , Carcinoma de Células Escamosas/diagnóstico , Técnicas Histológicas/métodos , Neoplasias Pulmonares/diagnóstico , Carcinoma de Pequenas Células do Pulmão/diagnóstico , Adenocarcinoma/patologia , Adenocarcinoma/cirurgia , Adulto , Idoso , Biópsia por Agulha Fina , Broncoscopia , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/cirurgia , Diagnóstico Diferencial , Feminino , Humanos , Pulmão/diagnóstico por imagem , Pulmão/patologia , Pulmão/cirurgia , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/cirurgia , Masculino , Pessoa de Meia-Idade , Pneumonectomia , Pneumologistas , Estudos Retrospectivos , Sensibilidade e Especificidade , Carcinoma de Pequenas Células do Pulmão/patologia , Carcinoma de Pequenas Células do Pulmão/cirurgia
2.
Nat Commun ; 11(1): 4686, 2020 09 17.
Artigo em Inglês | MEDLINE | ID: mdl-32943633

RESUMO

Electrophysiology provides a direct readout of neuronal activity at a temporal precision only limited by the sampling rate. However, interrogating deep brain structures, implanting multiple targets or aiming at unusual angles still poses significant challenges for operators, and errors are only discovered by post-hoc histological reconstruction. Here, we propose a method combining the high-resolution information about bone landmarks provided by micro-CT scanning with the soft tissue contrast of the MRI, which allowed us to precisely localize electrodes and optic fibers in mice in vivo. This enables arbitrating the success of implantation directly after surgery with a precision comparable to gold standard histology. Adjustment of the recording depth with micro-drives or early termination of unsuccessful experiments saves many working hours, and fast 3-dimensional feedback helps surgeons avoid systematic errors. Increased aiming precision enables more precise targeting of small or deep brain nuclei and multiple targeting of specific cortical or hippocampal layers.


Assuntos
Encéfalo/diagnóstico por imagem , Eletrodos Implantados , Processamento de Imagem Assistida por Computador/métodos , Fibras Ópticas , Microtomografia por Raio-X/métodos , Animais , Comportamento Animal , Encéfalo/patologia , Mapeamento Encefálico , Hipocampo/diagnóstico por imagem , Hipocampo/patologia , Hipocampo/cirurgia , Técnicas Histológicas/métodos , Imagem por Ressonância Magnética/métodos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Modelos Animais , Silício , Técnicas Estereotáxicas
3.
Rev. esp. patol ; 53(3): 188-192, jul.-sept. 2020. ilus
Artigo em Inglês | IBECS | ID: ibc-192406

RESUMO

The new coronavirus SARS-CoV-2, first identified in Wuhan, China in December, 2019, can cause Severe Acute Respiratory Syndrome (SARS) with massive alveolar damage and progressive respiratory failure. We present the relevant autopsy findings of the first patient known to have died from COVID19 pneumonia in Spain, carried out on the 14th of February, 2020, in our hospital (Hospital Arnau de Vilanova-Lliria, Valencia). Histological examination revealed changes typical of diffuse alveolar damage (DAD) in both the exudative and proliferative phase of acute lung injury. Intra-alveolar multinucleated giant cells, smudge cells and vascular thrombosis were present. The diagnosis was confirmed by reverse real-time PCR assay on a throat swab sample taken during the patient's admission. The positive result was reported fifteen days subsequent to autopsy


El nuevo coronavirus SARS-CoV-2, identificado inicialmente en China en diciembre de 2019 puede cursar con un Síndrome Respiratorio Agudo Severo (SARS) con daño alveolar masivo y fracaso respiratorio progresivo. Presentamos los hallazgos más relevantes encontrados en la autopsia clínica efectuada en nuestro hospital (Hospital Arnau de Vilanova-Lliria de Valencia) a fecha de 14 de febrero de 2020, al primer paciente fallecido conocido en España por neumonía COVID-19. A nivel pulmonar, la autopsia revela cambios típicos de daño alveolar difuso (DAD) en fase exudativa y fase proliferativa. Se observan células multinucleadas gigantes, células tipo smudge intraalveolares y trombosis vasculares. El diagnóstico microbiológico confirmativo mediante PCR se realizó 15 días después de la autopsia sobre la muestra faríngea del enfermo tomada durante su ingreso


Assuntos
Humanos , Masculino , Idoso , Autopsia/métodos , Infecções por Coronavirus/mortalidade , Síndrome Respiratória Aguda Grave/mortalidade , Vírus da SARS/isolamento & purificação , Técnicas Histológicas/métodos , Alvéolos Pulmonares/patologia , Espanha/epidemiologia , Causas de Morte , Pandemias
4.
Rev. esp. patol ; 53(3): 182-187, jul.-sept. 2020. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-192407

RESUMO

We describe the implementation of a COVID-19 Autopsy Programme in our Hospital, report the main findings from the first autopsy of the programme and briefly review the reports of lung pathology of these patients


En este artículo presentamos el proceso de implementación de un Programa de Autopsias COVID-19 en nuestro hospital, presentamos los principales hallagos de la primera autopsia realizada y revisamos brevemente la patología pulmonar publicada previamente en estos pacientes


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Embolia Pulmonar/complicações , Autopsia/estatística & dados numéricos , Causas de Morte , Infecções por Coronavirus/patologia , Alvéolos Pulmonares/patologia , Síndrome Respiratória Aguda Grave/complicações , Vírus da SARS/isolamento & purificação , Apneia Obstrutiva do Sono/complicações , Hipertensão/complicações , Reação em Cadeia da Polimerase/métodos , Esteroides/uso terapêutico , Pandemias , Técnicas Histológicas/métodos , Espanha/epidemiologia
5.
PLoS One ; 15(5): e0231602, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32469877

RESUMO

Reversible Data Hiding (RDH) techniques have gained popularity over the last two decades, where data is embedded in an image in such a way that the original image can be restored. Earlier works on RDH was based on the Image Histogram Modification that uses the peak point to embed data in the image. More recent works focus on the Difference Image Histogram Modification that exploits the fact that the neighbouring pixels of an image are highly correlated and therefore the difference of image makes more space to embed large amount of data. In this paper we propose a framework to increase the embedding capacity of reversible data hiding techniques that use a difference of image to embed data. The main idea is that, instead of taking the difference of the neighboring pixels, we rearrange the columns (or rows) of the image in a way that enhances the smooth regions of an image. Any difference based technique to embed data can then be used in the transformed image. The proposed method is applied on different types of images including textures, patterns and publicly available images. Experimental results demonstrate that the proposed method not only increases the message embedding capacity of a given image by more than 50% but also the visual quality of the marked image containing the message is more than the visual quality obtained by existing state-of-the-art reversible data hiding technique. The proposed technique is also verified by Pixel Difference Histogram (PDH) Stegoanalysis and results demonstrate that marked images generated by proposed method is undetectable by PDH analysis.


Assuntos
Algoritmos , Segurança Computacional/normas , Simulação por Computador , Bases de Dados Factuais , Técnicas Histológicas/métodos , Processamento de Imagem Assistida por Computador/métodos , Humanos , Registros
6.
PLoS One ; 15(3): e0229041, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32130242

RESUMO

METHODS: Muscle sections were stained for cell boundary (laminin) and myofiber type (myosin heavy chain isoforms). Myosoft, running in the open access software platform FIJI (ImageJ), was used to analyze myofiber size and type in transverse sections of entire gastrocnemius/soleus muscles. RESULTS: Myosoft provides an accurate analysis of hundreds to thousands of muscle fibers within 25 minutes, which is >10-times faster than manual analysis. We demonstrate that Myosoft is capable of handling high-content images even when image or staining quality is suboptimal, which is a marked improvement over currently available and comparable programs. CONCLUSIONS: Myosoft is a reliable, accurate, high-throughput, and convenient tool to analyze high-content muscle histology. Myosoft is freely available to download from Github at https://github.com/Hyojung-Choo/Myosoft/tree/Myosoft-hub.


Assuntos
Algoritmos , Ensaios de Triagem em Larga Escala/métodos , Técnicas Histológicas/métodos , Processamento de Imagem Assistida por Computador/métodos , Músculo Esquelético/patologia , Software , Anatomia Transversal/métodos , Animais , Tamanho Celular , Aprendizado de Máquina , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Fibras Musculares Esqueléticas/citologia , Fibras Musculares Esqueléticas/patologia , Músculo Esquelético/citologia , Reprodutibilidade dos Testes
7.
Virchows Arch ; 477(1): 103-110, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32144540

RESUMO

INTRODUCTION: Two types of testicular teratomas are distinguished by the current WHO classification. Prepubertal-type teratomas are benign, while postpubertal-type teratomas are considered malignant with metastatic potential, and are associated with germ cell neoplasia in situ. Prepubertal-type cases have been reported in the adult testis potentially causing confusion and overtreatment. Demonstration of the absence of 12p abnormalities with fluorescence in situ hybridization may facilitate diagnosis. Recently, IMP3 has emerged as a potential marker of malignancy in this context. AIMS: The aim of this study was to assess histological characteristics, IMP3 expression and the presence of 12p abnormalities of pure testicular teratomas. RESULTS: Thirty-seven cases were studied, 7 patients were children and 30 were adults. Six out of 7 pediatric cases showed no 12p abnormality and were IMP3 positive. Seventy-four percent and 79% of adult cases showed 12p abnormalities and IMP3 expression, respectively. Negative cases were not associated with in situ neoplasia or metastasis, they were smaller (mean, 14 vs 39 mm), showed less histological diversity (2.4 vs 4.0 types of tissues on average) compared to positive cases. CONCLUSION: Our study provides further evidence that prepubertal-type (type I) teratomas may appear in adult testes, thus teratomas in adults may be either benign (type I) or malignant (type II). IMP3 expression may aid the distinction between type I and type II teratomas of the postpubertal testis even when GCNIS and 12p status cannot be assessed.


Assuntos
Neoplasias Embrionárias de Células Germinativas/patologia , Teratoma/patologia , Neoplasias Testiculares/patologia , Adolescente , Adulto , Biomarcadores Tumorais/metabolismo , Criança , Cromossomos Humanos Par 12 , Feminino , Técnicas Histológicas/métodos , Humanos , Hibridização in Situ Fluorescente/métodos , Masculino , Pessoa de Meia-Idade , Proteínas de Ligação a RNA/metabolismo
9.
Micron ; 132: 102841, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32062296

RESUMO

The histological study of hard pieces such as tendons and calcified lesions and tissues is a field that has been gaining increased attention owing to the rapid development of implantable prostheses, among other factors. In these studies, serial sectioning is utilized to detect areas of interest throughout the entire piece, as it enables the application of the appropriate light and electron microscopy techniques in these areas. We propose the "three-sectioning method" that subjects the pieces to three consecutive cycles of embedding and sectioning to localize and study the areas of interest, as an efficient technique for these histological studies. The pieces were first embedded in epoxy resin and then cut into thick sections (approximately 300 µm) for the first cycle. Next, areas of interest selected on these thick sections were re-embedded in epoxy resin to be sectioned again (second sectioning) to obtain a series of semithin sections (1-3 µm). These semithin sections are usually studied using the most relevant techniques for light microscopy. Smaller areas of interest are selected to be cut into ultrathin sections (60-90 nm) for transmission electron microscopy. If necessary, the selected areas of the semithin sections can be embedded again, and then cut into new ultrathin sections. The different kinds of sections we have described here may also be studied using scanning electron microscopy. This systematic method facilitates correlative microscopy from lower to higher magnifications along with the usage of a broad variety of histological techniques including electron microscopy.


Assuntos
Técnicas Histológicas/métodos , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Microtomia/métodos , Manejo de Espécimes/métodos , Animais , Osso e Ossos/ultraestrutura , Resinas Epóxi , Feminino , Masculino , Ratos Wistar , Tendões/ultraestrutura
10.
Sci Rep ; 10(1): 1504, 2020 01 30.
Artigo em Inglês | MEDLINE | ID: mdl-32001752

RESUMO

Histopathological classification of gastric and colonic epithelial tumours is one of the routine pathological diagnosis tasks for pathologists. Computational pathology techniques based on Artificial intelligence (AI) would be of high benefit in easing the ever increasing workloads on pathologists, especially in regions that have shortages in access to pathological diagnosis services. In this study, we trained convolutional neural networks (CNNs) and recurrent neural networks (RNNs) on biopsy histopathology whole-slide images (WSIs) of stomach and colon. The models were trained to classify WSI into adenocarcinoma, adenoma, and non-neoplastic. We evaluated our models on three independent test sets each, achieving area under the curves (AUCs) up to 0.97 and 0.99 for gastric adenocarcinoma and adenoma, respectively, and 0.96 and 0.99 for colonic adenocarcinoma and adenoma respectively. The results demonstrate the generalisation ability of our models and the high promising potential of deployment in a practical histopathological diagnostic workflow system.


Assuntos
Neoplasias do Colo/classificação , Interpretação de Imagem Assistida por Computador/métodos , Neoplasias Gástricas/classificação , Área Sob a Curva , Inteligência Artificial , Biópsia , Colo/patologia , Neoplasias do Colo/patologia , Aprendizado Profundo , Diagnóstico por Computador/métodos , Técnicas Histológicas/métodos , Humanos , Aprendizado de Máquina , Redes Neurais de Computação , Estômago/patologia , Neoplasias Gástricas/patologia
11.
Nat Commun ; 11(1): 822, 2020 02 13.
Artigo em Inglês | MEDLINE | ID: mdl-32054838

RESUMO

High-grade serous ovarian carcinoma is characterised by TP53 mutation and extensive chromosome instability (CIN). Because our understanding of CIN mechanisms is based largely on analysing established cell lines, we developed a workflow for generating ex vivo cultures from patient biopsies to provide models that support interrogation of CIN mechanisms in cells not extensively cultured in vitro. Here, we describe a "living biobank" of ovarian cancer models with extensive replicative capacity, derived from both ascites and solid biopsies. Fifteen models are characterised by p53 profiling, exome sequencing and transcriptomics, and karyotyped using single-cell whole-genome sequencing. Time-lapse microscopy reveals catastrophic and highly heterogeneous mitoses, suggesting that analysis of established cell lines probably underestimates mitotic dysfunction in advanced human cancers. Drug profiling reveals cisplatin sensitivities consistent with patient responses, demonstrating that this workflow has potential to generate personalized avatars with advantages over current pre-clinical models and the potential to guide clinical decision making.


Assuntos
Bancos de Espécimes Biológicos , Mitose/genética , Neoplasias Ovarianas/genética , Neoplasias Ovarianas/patologia , Instabilidade Cromossômica , Resistencia a Medicamentos Antineoplásicos , Feminino , Expressão Gênica , Perfilação da Expressão Gênica , Técnicas Histológicas/métodos , Humanos , Imageamento Tridimensional , Hibridização in Situ Fluorescente , Técnicas In Vitro , Cariotipagem , Modelos Biológicos , Mutação , Neoplasias Ovarianas/tratamento farmacológico , Paclitaxel/farmacologia , Análise de Célula Única , Imagem com Lapso de Tempo , Proteína Supressora de Tumor p53/genética , Sequenciamento Completo do Exoma
12.
Nat Rev Neurosci ; 21(2): 61-79, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31896771

RESUMO

State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact tissues from individual organs and even to some entire mammals. When combined with light-sheet microscopy and automated approaches to image analysis, existing tissue-clearing methods can speed up and may reduce the cost of conventional histology by several orders of magnitude. In addition, tissue-clearing chemistry allows whole-organ antibody labelling, which can be applied even to thick human tissues. By combining the most powerful labelling, clearing, imaging and data-analysis tools, scientists are extracting structural and functional cellular and subcellular information on complex mammalian bodies and large human specimens at an accelerated pace. The rapid generation of terabyte-scale imaging data furthermore creates a high demand for efficient computational approaches that tackle challenges in large-scale data analysis and management. In this Review, we discuss how tissue-clearing methods could provide an unbiased, system-level view of mammalian bodies and human specimens and discuss future opportunities for the use of these methods in human neuroscience.


Assuntos
Técnicas Histológicas/métodos , Microscopia/métodos , Sistema Nervoso/citologia , Animais , Técnicas Histológicas/instrumentação , Humanos , Imageamento Tridimensional/métodos , Mamíferos , Microscopia/instrumentação , Neurociências
13.
J Vet Diagn Invest ; 32(1): 142-146, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31735129

RESUMO

Desmozoon lepeophtherii is a microsporidian associated with gill disease in farmed Atlantic salmon (Salmo salar). Detection of the parasite in histologic tissue sections is challenging using common histochemical stains given that the small, widely distributed parasite spores typically occur individually or in small clusters. We compared the ability of 4 histologic methods to detect D. lepeophtherii spores in serial sections of Atlantic salmon gill tissue: hematoxylin and eosin (H&E), Gram-Twort (GT), calcofluor white (CW), and immunohistochemistry (IHC). Using CW as a benchmark to calculate a relative ratio, IHC consistently detected more spores than CW (median: 1.3), followed by GT (median: 0.2) and H&E (median: 0.1). IHC detected significantly more spores than GT (p < 0.05) and H&E (p < 0.05), and GT more than H&E (p < 0.05). We found significant underestimation of numbers of microsporidia spores in gill disease in Atlantic salmon using conventional histochemical stains and recommend the use of CW or IHC to detect the parasite in tissue sections.


Assuntos
Doenças dos Peixes/microbiologia , Brânquias/microbiologia , Técnicas Histológicas/veterinária , Microsporídios/isolamento & purificação , Microsporidiose/veterinária , Salmo salar/microbiologia , Animais , Doenças dos Peixes/diagnóstico , Doenças dos Peixes/patologia , Técnicas Histológicas/métodos , Técnicas Histológicas/normas , Microsporidiose/diagnóstico , Microsporidiose/microbiologia , Esporos Fúngicos/isolamento & purificação
14.
Clin Anat ; 33(1): 108-112, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31576597

RESUMO

Deplastination is the process of reversing plastination such that a plastinated specimen can be reverted to its raw nature. This would enable its use in the field of histopathology. The present study aims to ascertain if deplastinates can be used for histopathological studies after a time period. Tissue samples were taken from patients undergoing maxillofacial surgeries for oral carcinomas after obtaining written informed consent. The 12 specimens obtained were divided into two groups. One set of tissues was processed for paraffin embedding after 10% formalin fixation. The other set was plastinated by S10 silicon plastination. After 3 months, the plastinates were deplastinated using sodium methoxide and processed for routine hematoxylin and eosin staining, similar to the formalin fixed specimens. The slides were quantitatively assessed on parameters like tissue architecture, staining property, and intracellular structure. In addition, the slides were qualitatively evaluated by a pathologist who was blinded to the mode of preservation to see if identification of pathological features was possible on a deplastinated slide. The formalin preserved specimens and deplastinated tissue slides compared closely in all three parameters tested with the need to identify the endpoint of deplastination. Qualitatively, deplastination did not hamper identification of tissue pathology. Deplastination increases the scope of a stored plastinate by allowing histological studies in the future without the need for any preservatives or special storage equipment. It preserves structure and maintains tissue pathology. An improved method of ensuring the endpoint of deplastination needs to be identified. Clin. Anat. 32:108-112, 2019. © 2019 Wiley Periodicals, Inc.


Assuntos
Técnicas Histológicas/métodos , Inclusão em Parafina , Inclusão em Plástico , Plastinação , Fixação de Tecidos/métodos , Formaldeído , Humanos
15.
IEEE Trans Image Process ; 29(1): 2026-2036, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31634128

RESUMO

Most whole-slide histological images are stained with two or more chemical dyes. Slide stain separation or color deconvolution is a crucial step within the digital pathology workflow. In this paper, the blind color deconvolution problem is formulated within the Bayesian framework. Starting from a multi-stained histological image, our model takes into account both spatial relations among the concentration image pixels and similarity between a given reference color-vector matrix and the estimated one. Using Variational Bayes inference, three efficient new blind color deconvolution methods are proposed which provide automated procedures to estimate all the model parameters in the problem. A comparison with classical and current state-of-the-art color deconvolution algorithms using real images has been carried out demonstrating the superiority of the proposed approach.


Assuntos
Técnicas Histológicas/métodos , Processamento de Imagem Assistida por Computador/métodos , Algoritmos , Teorema de Bayes , Cor , Bases de Dados Factuais , Humanos , Pulmão/patologia
16.
Anat Histol Embryol ; 49(1): 90-96, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31489697

RESUMO

The aim of this research was to determine brain, cerebral hemispheres and cerebellum volume and volume ratios by using stereological methods and investigate morphological differences between female and male New Zealand rabbits. The study was applied on 14-month old (10 male and 10 female) New Zealand rabbits. The materials removed from the cavum cranii using dorsal approach. After following routine histological procedure, paraffin blocks were prepared and cut every seventieth section at 10 µm thickness. Slides were stained with Crossmon's triple stain and photographed. The sectional images obtained were transferred to ImageJ program to estimate grey and white matter volume on cerebral hemispheres and cerebellum with principle of Cavalieri. According to results, there was no asymmetry on the left and right cerebral hemispheres of New Zealand rabbits. In the total hemisphere volume calculated by Cavalieri principle, grey and white matter ratio was 81.57% and 18.43% in female, 82.80% and 17.20% in male. It was found that the white matter was significantly higher in females than males in cerebral hemispheres (p < .05). Also, it was found that grey and white matter ratio in total cerebellum volume was 67.82% and 32.18% in female, 67.94% and 32.06% in male respectively. It was determined that the females' white matter was larger than male rabbits in cerebellum (p < .05). It is thought that morphometric data obtained from this study will contribute to the existing anatomical knowledge and also considered as reference values in the clinical sciences.


Assuntos
Encéfalo/anatomia & histologia , Substância Cinzenta/anatomia & histologia , Coelhos/anatomia & histologia , Substância Branca/anatomia & histologia , Animais , Cerebelo/anatomia & histologia , Cérebro/anatomia & histologia , Feminino , Técnicas Histológicas/métodos , Masculino , Tamanho do Órgão , Valores de Referência
18.
Med Sci (Paris) ; 35(11): 871-879, 2019 Nov.
Artigo em Francês | MEDLINE | ID: mdl-31845879

RESUMO

One of the most fascinating aspects of the use of a laser beam in the field of biology has emerged with the development of devices able to perform fine dissections of biological tissues. Laser microdissection can collect phenotypically identical cells from tissue regions laid on a microscope slide in order to make differential molecular analyses on these microdissected cells. Laser microdissection can be used many areas including oncology to specify molecular mechanisms that enable to adapt a treatment related to diagnosis and research in biology, but also forensic science for tissue selection, neurology for post-mortem studies on patients with Alzheimer's disease, for clonality studies from cell cultures and cytogenetics to decipher chromosomal rearrangements. This technology represents the missing link between clinical observations and the intrinsic physiological mechanisms of biological tissues and its major applications will be addressed here.


Assuntos
Técnicas Histológicas/métodos , Microdissecção e Captura a Laser , Técnicas de Diagnóstico Molecular/métodos , Humanos
19.
J Vis Exp ; (152)2019 10 24.
Artigo em Inglês | MEDLINE | ID: mdl-31710040

RESUMO

We demonstrate a laboratory-based method combining X-ray microCT and nanoCT with a specific X-ray stain, which targets the cell cytoplasm. The described protocol is easy to apply, fast and suitable for larger soft-tissue samples. The presented methodology enables the characterization of crucial tissue structures in three dimensions and is demonstrated on a whole mouse kidney. The multiscale approach allows to image the entire mouse kidney and supports the selection of further volumes of interest, which are acquired with higher resolutions ranging into the nanometer range. Thereby, soft-tissue morphology with a similar detail level as the corresponding histological light microscopy images is reproduced. Deeper insights into the 3D configuration of tissue structures are achieved without impeding further investigations through histological methods.


Assuntos
Técnicas Histológicas/métodos , Imageamento Tridimensional/métodos , Animais , Camundongos , Amostragem , Coloração e Rotulagem
20.
Clin Neurol Neurosurg ; 186: 105544, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31605894

RESUMO

OBJECTIVE: The negative biopsy rate approaches 5% in the literature. In our institution, this rate was 2.6% (42/1638) over a ten-year period (2007-2016). We aimed to assess the diagnostic yield of intraoperative smear during stereotactic biopsies to reduce this negative biopsy rate. PATIENTS AND METHODS: We retrospectively analyzed all consecutive MRI-guided frame-based stereotactic biopsies for which an intraoperative histological smear was carried out, performed over 29 months from January 2017 to May 2019 at the Pitié-Salpêtrière University Hospital (Paris, France). RESULTS: 145 stereotactic biopsies for which an intraoperative histological smear was carried out were performed in 145 adult patients. Mean age at biopsy was 52.4 ± 12.2 years. Histological diagnoses encountered in this series were: primary or secondary cerebral neoplasm (90.3%), inflammatory diseases (4.8%) and infectious diseases (4.8%). All biopsies were contributory to diagnosis. The negative biopsy rate was therefore significantly lower in the patient group for which an intraoperative histological smear was carried out than in our historical control group (0% versus 2.6%, p = 0.04). CONCLUSION: Considering the diagnostic yield benefit contributed by the intraoperative histological smear, we advocate for its routine use during brain stereotactic biopsies.


Assuntos
Neoplasias Encefálicas/patologia , Encéfalo/patologia , Monitorização Neurofisiológica Intraoperatória/métodos , Técnicas Estereotáxicas , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Encéfalo/cirurgia , Neoplasias Encefálicas/cirurgia , Feminino , Técnicas Histológicas/métodos , Humanos , Biópsia Guiada por Imagem/métodos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
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