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1.
Biochem Med (Zagreb) ; 30(1): 010502, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31839720

RESUMO

Extravascular body fluids (EBF) analysis can provide useful information in the differential diagnosis of conditions that caused their accumulation. Their unique nature and particular requirements accompanying EBF analysis need to be recognized in order to minimize possible negative implications on patient safety. This recommendation was prepared by the members of the Working group for extravascular body fluid samples (WG EBFS). It is designed to address the total testing process and clinical significance of tests used in EBF analysis. The recommendation begins with a chapter addressing validation of methods used in EBF analysis, and continues with specific recommendations for serous fluids analysis. It is organized in sections referring to the preanalytical, analytical and postanalytical phase with specific recommendations presented in boxes. Its main goal is to assist in the attainment of national harmonization of serous fluid analysis and ultimately improve patient safety and healthcare outcomes. This recommendation is intended to all laboratory professionals performing EBF analysis and healthcare professionals involved in EBF collection and processing. Cytological and microbiological evaluations of EBF are beyond the scope of this document.


Assuntos
Líquidos Corporais/química , Técnicas de Laboratório Clínico/normas , Líquidos Corporais/metabolismo , Exsudatos e Transudatos/química , Exsudatos e Transudatos/metabolismo , Guias como Assunto , Humanos , Segurança do Paciente , Derrame Pleural/diagnóstico , Garantia da Qualidade dos Cuidados de Saúde , Sociedades Médicas , Manejo de Espécimes/normas
2.
Rev Bras Epidemiol ; 22Suppl 02(Suppl 02): e190002.supl.2, 2019.
Artigo em Português, Inglês | MEDLINE | ID: mdl-31596373

RESUMO

INTRODUCTION: This article aims to estimate reference values for laboratory tests of cholesterol, glycosylated hemoglobin and creatinine for the Brazilian adult population. METHODS: A descriptive study carried out with laboratory data from the National Health Survey (Pesquisa Nacional de Saúde - PNS). Samples of blood and urine were collected in a PNS subsample of 8,952 individuals aged 18 years old or older. To determine the reference values, exclusion criteria were applied: presence of previous diseases and outliers, defined by values outside the range estimated by the mean ± 1.96 × standard deviation. Subsequently, reference values were calculated according to gender, age group and race/skin color. RESULTS: Differences in reference values according to gender were observed. Women had higher values of total cholesterol, LDL-c and HDL-c. Glycosylated hemoglobin showed similar values in relation to gender, and creatinine was higher among men. The mean reference values were higher in the elderly population, aged 60 years old or older. The mean, lower and upper limits of total cholesterol and fractions of non-white people were slightly lower. There was no difference according to race/skin color for glycosylated hemoglobin and creatinine. CONCLUSION: The establishment of national reference parameters for laboratory tests, adapted to the sociodemographic and geographic characteristics, provides relevant information for evaluation of diagnosis and treatment of chronic diseases in Brazil.


Assuntos
Colesterol/sangue , Técnicas de Laboratório Clínico/normas , Creatinina/análise , Hemoglobina A Glicada/análise , Adolescente , Adulto , Grupo com Ancestrais do Continente Africano , Brasil , Grupo com Ancestrais do Continente Europeu , Feminino , Inquéritos Epidemiológicos/métodos , Inquéritos Epidemiológicos/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Fatores Sexuais , Adulto Jovem
3.
Rev Bras Epidemiol ; 22Suppl 02(Suppl 02): E190003.SUPL.2, 2019.
Artigo em Português, Inglês | MEDLINE | ID: mdl-31596374

RESUMO

OBJECTIVE: To describe reference values for blood counts obtained from laboratory tests in the Brazilian adult population according to laboratory results from the National Health Survey (Pesquisa Nacional de Saúde - PNS), by gender, age group and skin color. METHODS: The initial sample consisted of 8,952 adults. To determine the reference values, individuals with prior diseases and outliers were excluded. Mean values, standard deviation and limits were stratified by gender, age group and skin color. RESULTS: For red blood cells, men presented a mean value of 5.0 million per mm3 (limits: 4.3-5.8) and women, 4.5 million per mm3 (limits: 3.9-5.1). Hemoglobin levels were higher among men with a mean of 14.9 g/dL (13.0-16.9), and in women, 13.2 g/dL (11.5-14.9). The mean number of white blood cells among men was 6.142/mm3 (2.843-9.440) and 6.426/mm3 (2.883-9.969) for women. Other parameters showed close values between the genders. Regarding age groups and skin color, mean values, standard deviation and limits of the exams presented small variations. CONCLUSION: Hematological reference values based on the national survey allow for the establishment of specific reference limits for gender, age and skin color. The results presented here may contribute to the establishment of better evidence and criteria for the care, diagnosis and treatment of diseases.


Assuntos
Contagem de Células Sanguíneas/normas , Técnicas de Laboratório Clínico/normas , Inquéritos Epidemiológicos/normas , Adolescente , Adulto , Fatores Etários , Brasil/etnologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Fatores Sexuais , Adulto Jovem
4.
Malar J ; 18(1): 300, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31477111

RESUMO

BACKGROUND: The ability to report vaccine-induced IgG responses in terms of µg/mL, as opposed arbitrary units (AU), enables a more informed interpretation of the magnitude of the immune response, and better comparison between vaccines targeting different antigens. However, these interpretations rely on the accuracy of the methodology, which is used to generate ELISA data in µg/mL. In a previous clinical trial of a vaccine targeting the apical membrane antigen 1 (AMA1) from Plasmodium falciparum, three laboratories (Oxford, NIH and WRAIR) reported ELISA data in µg/mL that were correlated but not concordant. This current study sought to harmonize the methodology used to generate a conversion factor (CF) for ELISA analysis of human anti-AMA1 IgG responses across the three laboratories. METHODS: Purified IgG was distributed to the three laboratories and, following a set protocol provided by NIH, AMA1-specific human IgG was affinity purified. A new "harmonized CF" was generated by each laboratory using their in-house ELISA, and the original clinical trial ELISA data were re-analysed accordingly. RESULTS: Statistical analysis showed that the data remained highly correlated across all three laboratories, although only Oxford and NIH were able to harmonize their CF for ELISA and generate concordant data. CONCLUSIONS: This study enabled two out of the three laboratories to harmonize their µg/mL readouts for the human anti-AMA1 IgG ELISA, but results reported from WRAIR are ~ twofold higher. Given the need to validate such information for each species and antigen of interest, it is important to bear in mind these likely differences when interpreting µg/mL ELISA data in the future.


Assuntos
Anticorpos Antiprotozoários/análise , Técnicas de Laboratório Clínico/normas , Ensaio de Imunoadsorção Enzimática/normas , Imunoglobulina G/análise , Vacinas Antimaláricas/imunologia , Anticorpos Antiprotozoários/imunologia , Antígenos de Protozoários/imunologia , Ensaios Clínicos como Assunto , Humanos , Imunoglobulina G/imunologia , Malária Falciparum/prevenção & controle , Proteínas de Membrana/imunologia , Plasmodium falciparum/imunologia , Proteínas de Protozoários/imunologia
8.
Rev Assoc Med Bras (1992) ; 65(6): 745-754, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31340297

RESUMO

The Guidelines Project, an initiative of the Brazilian Medical Association, aims to combine information from the medical field in order to standardize producers to assist the reasoning and decision-making of doctors. The information provided through this project must be assessed and criticized by the physician responsible for the conduct that will be adopted, depending on the conditions and the clinical status of each patient.


Assuntos
Técnicas de Laboratório Clínico/normas , Doenças Sexualmente Transmissíveis/diagnóstico , Brasil , Tomada de Decisão Clínica , Técnicas de Laboratório Clínico/métodos , Humanos , Fatores de Risco , Sensibilidade e Especificidade
9.
Diagn Microbiol Infect Dis ; 95(2): 191-194, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31296359

RESUMO

This study assessed the capacity of public secondary facility-based laboratories in conducting diagnostic tests for selected epidemic-prone diseases in Oyo State, Nigeria. A descriptive cross-sectional study was conducted in 17 secondary facility-based laboratories in Oyo State. Capacity was assessed on a 100-point scale in which scores were rated low (≤49%), fair (50-79%) and good (≥80%). Diagnostic testing capacity for bacterial meningitis, cholera, and measles was "low" in all the laboratories. The reasons reported for laboratories not conducting diagnostic tests for the selected diseases included inadequate instruments, unavailable reagents, and clinicians' failure to request those diagnostic tests. Laboratory capacity to perform diagnostic tests for the selected diseases was low in Oyo State secondary hospitals. There is a need for the provision of modern instruments and reagents, as well as clinician laboratorian quality assurance programs, to improve diagnostic services relating to the selected diseases.


Assuntos
Epidemias , Laboratórios/estatística & dados numéricos , Adulto , Atitude do Pessoal de Saúde , Técnicas de Laboratório Clínico/classificação , Técnicas de Laboratório Clínico/normas , Técnicas de Laboratório Clínico/estatística & dados numéricos , Estudos Transversais , Feminino , Instalações de Saúde/classificação , Instalações de Saúde/normas , Pessoal de Saúde/estatística & dados numéricos , Humanos , Laboratórios/normas , Masculino , Pessoa de Meia-Idade , Nigéria
10.
Clin Lab ; 65(7)2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31307171

RESUMO

BACKGROUND: Verification of the performance of analytical platforms is indicated prior to adoption of new Technology for patient sample analysis. Acceptance criteria for the performance of coagulation analytical platforms are not always readily available and is complicated by the multiple assays and test principles in this section of the clinical laboratory. Coagulation samples are also prone to pre-analytical, post-sample collection variables potentially interfering with accuracy analysis. METHODS: This verification study assessed the accuracy of the automated STAGO STA-R Max® coagulation analyzers by means of a comparison study of results obtained on the previously validated STAGO STA-R Evolution® analyzer for 22 coagulation parameters on 40 individual patient samples for each parameter. Within- and between- run reproducibility on commercial control material, carry-over from abnormal to normal samples and the interference of bilirubin, hemoglobin and lipids on the chromogenic analytical channel were also assessed. Ongoing evaluation of the analyzer performance was assessed by External Quality Assurance (EQA) scheme participation. RESULTS: The reproducibility (precision) on 2 levels (Normal and Pathological) commercial control material was acceptable with co-efficient of variance (CV) results below the manufacturer target % CVs. The correlation study demonstrated accuracy of results obtained on the analyzers for all parameters except for D-dimers and coagulation Factor VII. Subsequent EQA performance for these two parameters were however satisfactory. Interference from bilirubin, hemoglobin and lipids did occur in the chromogenic channel. No clinically significant carry over from abnormal to normal samples were observed. CONCLUSIONS: The performance of the STAGO STA-R Max® analyzer is acceptable across the full coagulation test repertoire with the exception of the von Willebrand activity assay. Participation in EQA scheme assessments will be an integral part of ongoing monitoring of the performance of this automated analyzer.


Assuntos
Automação Laboratorial/instrumentação , Testes de Coagulação Sanguínea/instrumentação , Coagulação Sanguínea , Técnicas de Laboratório Clínico/instrumentação , Garantia da Qualidade dos Cuidados de Saúde , Automação Laboratorial/métodos , Automação Laboratorial/normas , Testes de Coagulação Sanguínea/métodos , Testes de Coagulação Sanguínea/normas , Serviços de Laboratório Clínico/normas , Técnicas de Laboratório Clínico/métodos , Técnicas de Laboratório Clínico/normas , Humanos , Laboratórios/normas , Reprodutibilidade dos Testes , África do Sul
11.
Clin Lab ; 65(7)2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31307182

RESUMO

BACKGROUND: The Mindray SAL 8000 is an integrated serum analyzer for photometric, electrochemical, and im-munological assays. The technical, analytical, and workflow performance of the system were evaluated in this study. METHODS: The technical evaluation was performed using protocols adopted from the guidelines of the China Food and Drug Administration (CFDA). The precision, linearity, interference, and method comparison were carried out according to the Clinical and Laboratory Standards Institute (CLSI) protocols. The verification of carryover and turnaround time were conducted using specimens containing different analytes. RESULTS: The technical performance was acceptable for all evaluated aspects. The repeatability and within-labora-tory coefficients of variation (CVs) ranged between 0.22% and 4.23% for routine chemistry and between 1.05% and 6.89% for immunochemistry, respectively. All evaluated analytes exhibited linearity over the ranges claimed by the manufacturer. Significant interferences were observed during low concentration TBIL and P measure-ments due to the presence of lipemia. Method comparisons showed good agreement with the comparison systems and with the correlation coefficients ≥ 0.988 except for anti-HBs (r = 0.812). No significant intra-module and inter-module carryovers were detected. For all the 1,220 samples, 25%, 54%, 63%, 79%, 91%, and 100% samples com-pleted analysis in 16.3 minutes, 30 minutes, 60 minutes, 120 minutes, 180 minutes, and 320 minutes, respectively. CONCLUSIONS: The Mindray SAL 8000 integrated system achieved optimal technical performance and met most of the criteria regarding analytical performance. The workflow study of the system met the turnaround time (TAT) requirements of laboratories. Therefore, it is a good candidate to be used in medium and large-sized laboratories.


Assuntos
Química Clínica/métodos , Técnicas de Laboratório Clínico/métodos , Imunoensaio/métodos , Testes Imunológicos/métodos , Química Clínica/normas , Técnicas de Laboratório Clínico/normas , Humanos , Imunoensaio/normas , Testes Imunológicos/normas , Reprodutibilidade dos Testes
12.
BMC Med Inform Decis Mak ; 19(1): 123, 2019 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-31269951

RESUMO

BACKGROUND: The autoverification system for coagulation consists of a series of rules that allow normal data to be released without manual verification. With new advances in medical informatics, the laboratory information system (LIS) has growing potential for the autoverification, allowing rapid and accurate verification of clinical laboratory tests. The purpose of the study is to develop and evaluate a LIS-based autoverification system for validation and efficiency. METHODS: Autoverification decision rules, including quality control, analytical error flag, critical value, limited range check, delta check and logical check, as well as patient's historical information, were integrated into the LIS. Autoverification limited range was constructed based on 5 and 95% percentiles. The four most commonly used coagulation assays, prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen (FBG), were followed by the autoverification protocols. The validation was assessed by the autoverification passing rate, the true-positive cases, the true-negative cases, the false-positive cases, the false-negative cases, the sensitivity and the specificity; the efficiency was evaluated in the turnaround time (TAT). RESULTS: A total of 157,079 historical test results of coagulation profiles from January 2016 to December 2016 were collected to determine the distribution intervals. The autoverification passing rate was 77.11% (29,165/37,821) based on historical patient data. In the initial test of the autoverification version in June 2017, the overall autoverification passing rate for the whole sample was 78.75% (11,257/14,295), with 892 true-positive cases, 11,257 true-negative cases, 2146 false-positive cases, no false-negative cases, sensitivity of 100% and specificity of 83.99%. After formal implementation of the autoverification system for 6 months, 83,699 samples were assessed. The average overall autoverification passing rate for the whole sample was 78.86% and the 95% confidence interval (CI) of the passing rate was [78.25, 79.59%]. TAT was reduced from 126 min to 101 min, which was statistically significant (P < 0.001, Mann-Whitney U test). CONCLUSIONS: The autoverification system for coagulation assays based on LIS can halt the samples with abnormal values for manual verification, guarantee medical safety, minimize the requirements for manual work, shorten TAT and raise working efficiency.


Assuntos
Testes de Coagulação Sanguínea , Sistemas de Informação em Laboratório Clínico , Técnicas de Laboratório Clínico , Aplicações da Informática Médica , Segurança , Testes de Coagulação Sanguínea/normas , Sistemas de Informação em Laboratório Clínico/normas , Técnicas de Laboratório Clínico/normas , Humanos , Segurança/normas , Desenho de Programas de Computador
13.
Comput Math Methods Med ; 2019: 1891569, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31275427

RESUMO

Diagnostic tests are approaches used in clinical practice to identify with high accuracy the disease of a particular patient and thus to provide early and proper treatment. Reporting high-quality results of diagnostic tests, for both basic and advanced methods, is solely the responsibility of the authors. Despite the existence of recommendation and standards regarding the content or format of statistical aspects, the quality of what and how the statistic is reported when a diagnostic test is assessed varied from excellent to very poor. This article briefly reviews the steps in the evaluation of a diagnostic test from the anatomy, to the role in clinical practice, and to the statistical methods used to show their performances. The statistical approaches are linked with the phase, clinical question, and objective and are accompanied by examples. More details are provided for phase I and II studies while the statistical treatment of phase III and IV is just briefly presented. Several free online resources useful in the calculation of some statistics are also given.


Assuntos
Técnicas de Laboratório Clínico/normas , Erros de Diagnóstico/prevenção & controle , Testes Diagnósticos de Rotina/métodos , Testes Diagnósticos de Rotina/normas , Estatística como Assunto , Estudos de Coortes , Análise Custo-Benefício , Estudos Transversais , Reações Falso-Positivas , Humanos , Internet , Curva ROC , Valores de Referência , Reprodutibilidade dos Testes , Projetos de Pesquisa
14.
Rev Chilena Infectol ; 36(2): 145-166, 2019 Apr.
Artigo em Espanhol | MEDLINE | ID: mdl-31344152

RESUMO

The confrontation of the differential and etiological diagnosis of the infectious diseases of cancer patients, including hematopoietic stem cells transplant (HSCT) recipients, must correspond to an informed, timely decision that directly affects medical behavior that determines a better survival and quality of life for patients. The main goal of this work was to contribute to the management of these patients developing a useful tool for the clinician to make these decisions. For that, infections were grouped by compromised systems, differentiating the possible etiological agents in bacteria, viruses, fungi and parasites, highlighting the relevant diagnostic tests, mentioning the recommended techniques together with the optimal sample type for proper processing. In addition, under each group of techniques we added the item "level of requirement" to suggest what, in the opinion of the authors and the existing evidence, must be mandatory to have at local level or can be derivable to another laboratory.


Assuntos
Infecção Hospitalar/diagnóstico , Infecção Hospitalar/microbiologia , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Laboratórios Hospitalares/normas , Neoplasias/complicações , Biópsia/normas , Técnicas de Laboratório Clínico/métodos , Técnicas de Laboratório Clínico/normas , Infecção Hospitalar/terapia , Exposição Ambiental/efeitos adversos , Humanos , Imunocompetência , Neoplasias/terapia
15.
Lakartidningen ; 1162019 Apr 15.
Artigo em Sueco | MEDLINE | ID: mdl-31192382

RESUMO

Many decisions within healthcare are based on results from laboratory tests. These results all have an uncertainty, which can be either neglectable or large enough to affect the clinical assessment. In external quality assessment (EQA) the laboratories make independent observations on the same control sample, with values unknown for the participants. The results can be used to determine if quality improvement is needed. We present EQA data for laboratory tests with three different measurement scales; quantitative, ordinal and nominal. Results from EQA should be used in the discussions of quality in test results and necessary analytical performance specifications should be agreed upon.


Assuntos
Serviços de Laboratório Clínico/normas , Técnicas de Laboratório Clínico/normas , Controle de Qualidade , Neoplasias da Mama/patologia , Hemoglobina A Glicada/análise , Humanos , Melanoma/patologia , Variações Dependentes do Observador , Garantia da Qualidade dos Cuidados de Saúde/métodos , Streptococcus pyogenes/isolamento & purificação , Neoplasias da Bexiga Urinária/patologia
16.
Lakartidningen ; 1162019 Apr 02.
Artigo em Sueco | MEDLINE | ID: mdl-31192386

RESUMO

The aim of automated reflex testing is to achieve more rational and cost-effective use of laboratory investigations in clinical practice. In order to make use of this cost-effectiveness, the new tests should have a short implementation phase and clear objective goals. To accomplish an equal health care, the usage of new tests should have minimal variations between different primary health care units. We started a new model in Ostergotland County regarding clinical usage of new tests by active follow-up in two levels, both primary health care unit level and individual physician level, during the implementation phase. This study shows that active follow-up and feedback resulted in both shorter implementation phase and minimal variation between different primary health care units.


Assuntos
Técnicas de Laboratório Clínico/normas , Testes Diagnósticos de Rotina/normas , Ácido Metilmalônico/análise , Tireotropina/análise , Vitamina B 12/análise , Técnicas de Laboratório Clínico/métodos , Testes Diagnósticos de Rotina/métodos , Humanos , Modelos Teóricos , Atenção Primária à Saúde , Suécia
17.
Med Mal Infect ; 49(5): 335-346, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31155367

RESUMO

The serodiagnosis of Lyme borreliosis is based on a two-tier strategy: a screening test using an immunoenzymatic technique (ELISA), followed if positive by a confirmatory test with a western blot technique for its better specificity. Lyme serology has poor sensitivity (30-40%) for erythema migrans and should not be performed. The seroconversion occurs after approximately 6 weeks, with IgG detection (sensitivity and specificity both>90%). Serological follow-up is not recommended as therapeutic success is defined by clinical criteria only. For neuroborreliosis, it is recommended to simultaneously perform ELISA tests in samples of blood and cerebrospinal fluid to test for intrathecal synthesis of Lyme antibodies. Given the continuum between early localized and disseminated borreliosis, and the efficacy of doxycycline for the treatment of neuroborreliosis, doxycycline is preferred as the first-line regimen of erythema migrans (duration, 14 days; alternative: amoxicillin) and neuroborreliosis (duration, 14 days if early, 21 days if late; alternative: ceftriaxone). Treatment of articular manifestations of Lyme borreliosis is based on doxycycline, ceftriaxone, or amoxicillin for 28 days. Patients with persistent symptoms after appropriate treatment of Lyme borreliosis should not be prescribed repeated or prolonged antibacterial treatment. Some patients present with persistent and pleomorphic symptoms after documented or suspected Lyme borreliosis. Another condition is eventually diagnosed in 80% of them.


Assuntos
Técnicas de Laboratório Clínico , Doença de Lyme , Doenças Transmitidas por Carrapatos , Animais , Técnicas de Laboratório Clínico/métodos , Técnicas de Laboratório Clínico/normas , Diagnóstico Diferencial , Progressão da Doença , França , Humanos , Doença de Lyme/complicações , Doença de Lyme/diagnóstico , Doença de Lyme/patologia , Doença de Lyme/terapia , Guias de Prática Clínica como Assunto , Sociedades Científicas/organização & administração , Sociedades Científicas/normas , Doenças Transmitidas por Carrapatos/complicações , Doenças Transmitidas por Carrapatos/diagnóstico , Doenças Transmitidas por Carrapatos/patologia , Doenças Transmitidas por Carrapatos/terapia
18.
Clin Lab ; 65(6)2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31232021

RESUMO

BACKGROUND: A quality medical laboratory service is an important part of the health care system. In developing countries like Ethiopia, the laboratory quality system remains weak due to several factors. Therefore, assessing the factors affecting quality of medical laboratory service is highly important in order to improve service quality. OBJECTIVE: Assessment of factors affecting the quality of medical laboratory service in Gondar town public health facilities, Amhara regional state, Northwest Ethiopia, 2018. METHODS: An institution based cross-sectional study was conducted at Gondar town governmental health facilities from March to April 2018. A pretested, self-administered, semi-structured questionnaire and checklist was used to collect the socio-demographic information of the study participants and to assess factors affecting the provision of quality medical laboratory services. Data were checked for completeness, entered, and analyzed using SPSS version 20 (IBM Corporation, Armonk, NY, USA). Data were reported in tables and figures. The strength of association between the dependent and independent variables was assessed by the chi-square test. A p-value < 0.05 was considered statistically significant. RESULTS: A total of 103 medical laboratory professionals participated in the study. Of these, 62 (60.2%) were males. The majority of the study participants, 63 (61.2%), were laboratory technologists. Of these participants, 72 (69.9%) did not attend laboratory refresher training and 93 (90.3%) of participants were not satisfied with their salary. Sixty-three (61.2%) of the participants reported that their laboratory did not provide quality laboratory service. Lack of quality and adequate equipment, non-adherence to standard operating procedures, no continuing professional development, unavailability of adequate supplies and reagents, no customer service management, no regular internal and external quality assessment activity, no diagnostic service for all requested tests, no result verification, and laboratory safety were the major factors significantly associated with poor quality laboratory service (p < 0.05). CONCLUSIONS: A high proportion of the study participants reported that their laboratory did not provide quality laboratory services. The shortage of adequate equipment, reagents, and lack of motivation and employees' recognitions are the major factors that affect the quality of laboratory services.


Assuntos
Serviços de Laboratório Clínico/normas , Técnicas de Laboratório Clínico/normas , Instalações de Saúde/normas , Pessoal de Laboratório Médico/normas , Saúde Pública/normas , Adulto , Técnicas de Laboratório Clínico/métodos , Estudos Transversais , Assistência à Saúde/normas , Assistência à Saúde/estatística & dados numéricos , Etiópia , Feminino , Instalações de Saúde/estatística & dados numéricos , Humanos , Masculino , Pessoal de Laboratório Médico/estatística & dados numéricos , Saúde Pública/métodos , Saúde Pública/estatística & dados numéricos , Controle de Qualidade , Inquéritos e Questionários , Adulto Jovem
19.
Adv Physiol Educ ; 43(3): 317-323, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31246509

RESUMO

Prelaboratory tasks are used to facilitate learning and introduce and provide context for laboratory work. The application of first-person perspective (FPP) technology may provide interesting new approaches to providing prelaboratory preparation. However, there is limited knowledge as to whether this perspective is useful or enjoyable for students preparing for laboratory tasks. The purpose of this study was to examine whether prelaboratory preparation, utilizing the FPP technique, was enjoyable and led to improvements in laboratory task-specific self-efficacy in comparison to the traditional text-only (TO) style. We observed that the FPP group found the style to be generally more enjoyable, entertaining, and generally fun compared with the TO group (5.3 ± 0.2 and 2.7 ± 0.3, respectively, P < 0.05). Furthermore, we found that the FPP group had a greater laboratory task-specific self-efficacy than their counterparts in the TO group, following the prelaboratory preparation (93.6 ± 1.6 and 83.5 ± 3.2, respectively, P < 0.05). We did not find any differences in scenario-based self-efficacy between the FPP and the TO group. Taken together, our data support the use of FPP videos as a novel, refreshing approach to prelaboratory preparation that builds self-efficacy in students performing laboratory tasks.


Assuntos
Técnicas de Laboratório Clínico/métodos , Avaliação Educacional/métodos , Autoeficácia , Estudantes/psicologia , Universidades , Técnicas de Laboratório Clínico/normas , Avaliação Educacional/normas , Humanos , Universidades/normas
20.
Ann Lab Med ; 39(6): 552-560, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31240883

RESUMO

BACKGROUND: In Korea, the Korean Laboratory Accreditation Program (KLAP) has set minimum standards for verification of clinical test performance. This verification process is time-consuming and labor-intensive when performed manually. We developed a free, statistical software program for KLAP, using the R language (R Foundation for Statistical Computing, Vienna, Austria). METHODS: We used CLSI guidelines for the algorithm. We built graphic user interfaces, including data input, with Embarcadero Delphi EX4 (Embarcadero Technologies, Inc., Texas, USA). The R Base Package and MCR Package for Method Comparison Regression were used to implement statistical and graphical procedures. RESULTS: Our program LaboStats has six modules: parallel test, linearity, method comparison, precision, reference interval, and cutoff. Data can be entered into the field either manually or by copying and pasting from an MS Excel worksheet. Users can print out precise reports. CONCLUSIONS: LaboStats can be useful for evaluating clinical test performance characteristics and preparing documents requested by KLAP.


Assuntos
Laboratórios/normas , Software , Acreditação , Algoritmos , Técnicas de Laboratório Clínico/normas , Humanos , Valores de Referência , Reprodutibilidade dos Testes , República da Coreia
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