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1.
Folia Microbiol (Praha) ; 64(5): 601-602, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31385158

RESUMO

The following series of articles form a special issue organized by the Algatech Center of the Institute of Microbiology CAS dedicated to the memory of Dr. Ivan Setlík.


Assuntos
Cianobactérias/metabolismo , Microbiologia/história , Biocombustíveis/história , Cianobactérias/genética , Cianobactérias/efeitos da radiação , Tchecoslováquia , História do Século XX , História do Século XXI , Fotossíntese
3.
Acta Med Acad ; 48(1): 121-126, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31264440

RESUMO

This biographical note details Anna Bayerová's (1853-1924) activities as the first female Austro-Hungarian health officer in 1878 to1918 occupied Bosnia and Herzegovina (BH). Anna Bayerová is known as a heroine of Czech feminism and the 'first Czech female physician', though she only practised in the Czech lands from 1913 to 1916. In 1891, Bayerová was enrolled as the first Austro-Hungarian female health officer and assigned to treat Muslim women in the district of Tuzla, Bosnia. She pursued this mission for the first three months of 1892, had herself transferred to Sarajevo in the summer, and soon thereafter quitted the service. Her biographers point to a series of political and personal motivations to abandon her mission in Bosnia, which, from the viewpoint of Czech feminists, included fulfilling her professional duties in an exemplary way. She spent most of her professional life as a physician in Switzerland and did not request Austrian recognition of her medical degree until 1913. Bayerová died in Prague in 1924. Conclusion. Bayerová, partly for political reasons and partly due to her panic-fuelled fear of catching tuberculosis, quitted her role as the first Austro-Hungarian female health officer in BH soon after her arrival in 1892.


Assuntos
Serviços de Saúde Comunitária/história , Grupos Étnicos/história , Médicas/história , Áustria-Hungria , Bósnia e Herzegóvina , Tchecoslováquia , Feminino , Feminismo/história , História do Século XIX , História do Século XX , Humanos , Islamismo , Suíça
4.
Mycotoxin Res ; 35(4): 391-403, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31254204

RESUMO

The Czech Republic occupies the first place in the world in the frequency of renal and other urinary tract tumours, but their aetiology is unknown. To explore whether carcinogenic and nephrotoxic mycotoxins may contribute to kidney diseases in the Czech population, biomarkers of ochratoxin A (OTA) and citrinin (CIT) exposure were determined in biological specimens from a cohort of 50 patients with malignant renal tumours. Biomarker analyses in blood and urine samples used validated targeted methods for measuring OTA and CIT plus dihydrocitrinone (DH-CIT) after enrichment of analytes by specific immunoaffinity clean-up. OTA and CIT plus its metabolite DH-CIT were frequently detected in patient urine samples (OTA 62%; CIT 91%; DH-CIT 100%). The concentration ranges in urine were 1-27.8 ng/L for OTA, 2-87 ng/L for CIT and 2-160 ng/L for DH-CIT. The analyses of blood samples revealed also a frequent co-occurrence of OTA and CIT, in the ranges of 40-870 ng/L serum for OTA and 21-182 ng/L plasma for CIT. This first analysis of biomarkers in blood and urine samples of Czech patients revealed no major differences in comparison with published data for the general healthy Czech and European populations. Nonetheless, a frequent co-occurrence of CIT and OTA biomarkers in patient samples may be of interest with regard to potential interactions with other risk factors for renal disease.


Assuntos
Neoplasias Renais/química , Neoplasias Renais/urina , Micotoxinas/urina , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Biomarcadores/urina , Cromatografia Líquida , Citrinina/sangue , Citrinina/urina , Estudos de Coortes , Tchecoslováquia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Micotoxinas/sangue , Ocratoxinas/sangue , Ocratoxinas/urina , Espectrometria de Massas em Tandem
5.
J Tissue Viability ; 28(3): 139-143, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31000336

RESUMO

OBJECTIVE: The study aimed to determine the clinical effectiveness of honey dressings in the management of non-healing wounds in elderly persons receiving home care. MATERIAL AND METHODS: Design: a prospective interventional study. The sample comprised 40 Czech home care clients (aged over 65 years) with non-healing wounds who were randomly assigned to two groups. Wounds were treated with honey (intervention group) or conventional (controls) dressings. Each wound was studied for three months. A detailed description of a wound (location, size, wound bed, edges, amount of exudate, odor, adjacent skin) were recorded. Wounds were assessed with the Wound Healing Continuum and pain intensity with the Visual Analog Scale. RESULTS: Over the 3-month period, 16 (80%) individuals in the intervention group had their wounds completely healed, as compared with only six (30%) controls. There was no statistically significant difference in wound size between the groups on Day 1 (p = 0.1801). Ninety days later, the difference in wound size between the groups was statistically significant (p = 0.0041). There was a statistically significant difference in pain intensity between the two groups (p = 0.0007), with higher pain scores being indicated by controls. CONCLUSION: The study results showed that the application of honey dressings to non-healing wounds resulted in faster healing, wound size reduction and lower pain intensity.


Assuntos
Mel , Cicatrização/efeitos dos fármacos , Idoso , Idoso de 80 Anos ou mais , Curativos Biológicos/normas , Curativos Biológicos/estatística & dados numéricos , Tchecoslováquia , Feminino , Geriatria/instrumentação , Geriatria/métodos , Serviços de Assistência Domiciliar/normas , Serviços de Assistência Domiciliar/estatística & dados numéricos , Humanos , Masculino , Estudos Prospectivos , Fatores de Tempo , Cicatrização/fisiologia
6.
J Lesbian Stud ; 23(1): 52-67, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30714496

RESUMO

A Czech Holocaust survivor rescued by a Kindertransport in 1939; a long-lost Torah scroll, rediscovered in 1964, from a Jewish community wiped out in World War II; a German American lesbian who converted to Judaism in 2001. Three disparate stories, unfolding decades apart, converge in one memorable encounter, a Kristallnacht commemoration in Los Angeles organized by Beth Chayim Chadashim (BCC), the world's first LGBTQ synagogue, which leads to an enduring friendship and fresh insight into contemporary queer Jewish life. In this personal essay, longtime BCC member Sylvia Sukop interweaves history and autobiography to explore the beauty and power of ritual, the resonance of the "Choose life" passage in Deuteronomy that her congregation reads from its rescued Czech scroll every Yom Kippur, and the many forms that good deeds and survival can take. Progressive faith communities, the author suggests, and the traditions in which they are rooted make space to witness and affirm the fullness of one another's humanity, bridging differences and fostering unexpected kinship in a brutally divisive world.


Assuntos
Holocausto/história , Homossexualidade Feminina/história , Judeus/história , Minorias Sexuais e de Gênero/história , Sobreviventes/história , Tchecoslováquia , Feminino , História do Século XX , Homossexualidade Feminina/psicologia , Humanos , Judeus/psicologia , Minorias Sexuais e de Gênero/psicologia , Sobreviventes/psicologia
7.
Bratisl Lek Listy ; 120(1): 3-8, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30685985

RESUMO

The first human-to-human heart transplantation in Czechoslovakia, and the 25th transplantation in the world, was performed in Bratislava, the second largest city in Czechoslovakia on July 9, 1968. The operation was carried out by a team led by Professors Karol Siska and Ladislav Kuzela at the second Surgical Clinic at the Comenius University of the Medical Faculty in Bratislava, Partizanska Street-only seven months after the first heart transplantation performed by Dr. Christiaan Barnard in Cape Town. Other members of the team in Bratislava included surgery recipients Siska, Kuzela, Pivkova, Holoman; surgery donors Schnorrer, Kuzela, Holoman; an extracorporeal circulation team of Treger, Carsky, Podolay; anesthesiologists Sobesky and Neumanova; operating room nurses Machkova, Homerova, Kralova, and operating room laboratory technician Malinova. The donor was P.V., a 46-year-old man, who suffered from a deadly brain trauma. The recipient was S.H., a 54-year-old woman with a failing heart, heavily affected diseased lungs, kidneys and liver. Her heart began to work, but lasted only for five hours. (Additional members of the team, Prof. Simkovic and Drs. Silvay and Sujansky were in the USA at the moment of transplantation, in Houston and New York, subsequently) (Tab. 1, Fig. 2, Ref. 62). Keywords: first heart transplantation in Bratislava, 2nd Surgical Clinic at the Comenius University.


Assuntos
Transplante de Coração , Tchecoslováquia , Feminino , Transplante de Coração/história , História do Século XX , Humanos , Fígado , Masculino , Pessoa de Meia-Idade
9.
Acta Chir Belg ; 119(3): 166-175, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30010511

RESUMO

BACKGROUND: Colorectal cancer in pregnancy is rare, with an incidence of 0.8 per 100,000 pregnancies. Advanced disease (stage III or IV) is diagnosed more frequently in pregnant patients. We aimed to review all cases of colorectal cancer in pregnancy from the International Network on Cancer, Infertility and Pregnancy database in order to learn more about this rare disease and improve its management. METHODS: Data on the demographic features, symptoms, histopathology, diagnostic and therapeutic interventions and outcomes (obstetric, neonatal and maternal) were analysed. RESULTS: Twenty-seven colon and 14 rectal cancer cases were identified. Advanced disease was present in 30 patients (73.2%). During pregnancy, 21 patients (51.2%) received surgery and 12 patients (29.3%) received chemotherapy. Thirty-three patients (80.5%) delivered live babies: 21 by caesarean section and 12 vaginally. Prematurity rate was high (78.8%). Eight babies were small for gestational age (27.6%). Three patients (10.7%) developed recurrence of disease. Overall 2-year survival was 64.4%. CONCLUSION: Despite a more frequent presentation with advanced disease, colorectal cancer has a similar prognosis in pregnancy when compared with the general population. Diagnostic interventions and treatment should not be delayed due to the pregnancy but a balance between maternal and foetal wellbeing must always be kept in mind.


Assuntos
Neoplasias Colorretais/cirurgia , Complicações Neoplásicas na Gravidez/cirurgia , Adulto , Peso ao Nascer , Quimioterapia Adjuvante , Estudos de Coortes , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/patologia , Terapia Combinada , Tchecoslováquia , Feminino , Humanos , Recém-Nascido , Recidiva Local de Neoplasia/mortalidade , Estadiamento de Neoplasias , Gravidez , Complicações Neoplásicas na Gravidez/mortalidade , Complicações Neoplásicas na Gravidez/patologia , Resultado da Gravidez , Sistema de Registros , Taxa de Sobrevida
10.
Transplant Proc ; 50(10): 3082-3087, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30577170

RESUMO

BACKGROUND: The selection of optimal donor is crucial for successful hematopoietic stem cell transplantation (HSCT). Thereby, it is appropriate to know, in addition to basic human leukocyte antigen (HLA) gene matches, other immunogenic or nonimmunogenic parameters predicting the outcome of transplant. OBJECTIVE: A unified approach is necessary to provide a comprehensive view of the patient-donor compatibility characterization outside of standard HLA genes. The approach should be applicable as a tool for optimizing procedures for extended donor typing and/or verification typing of a donor. METHODS: The study used the summary, unification, and innovation of existing practical knowledge and experience of the Czech National Marrow Donor Registry of various factors beyond HLA matching with impact on transplant outcome. RESULTS: An information technology system-implemented procedure (a verification algorithm) is presented as the decision support approach for prematurely discarding less suitable donors from the transplantation process. It is intended primarily for the transplant specialist to help establish optimal procedures for verifying and determining donor critical factors. CONCLUSIONS: A process defining HLAs, killer cell immunoglobulin-like receptors, and cytokine typing strategies was proposed to provide support to a transplant specialist in refining the choice of a suitable donor.


Assuntos
Algoritmos , Sistemas de Apoio a Decisões Clínicas , Seleção do Doador/métodos , Transplante de Células-Tronco Hematopoéticas/métodos , Tchecoslováquia , Antígenos HLA/imunologia , Teste de Histocompatibilidade/métodos , Humanos , Receptores KIR/imunologia , Sistema de Registros
12.
Ann Intern Med ; 169(9): 636-642, 2018 11 06.
Artigo em Inglês | MEDLINE | ID: mdl-30398637

RESUMO

Jeremy Swan and William Ganz developed their eponymous pulmonary artery (PA) catheter in the 1970s and, in the process, revolutionized measurement of cardiac output, pressures within the left side of the heart, and resistance in systemic and pulmonary circulations. Their invention enabled diagnostic measurements at the bedside and contributed to the birth of critical care medicine; technologic advances preceding the PA catheter generally could not be used at the bedside and required patients to be stable enough to be taken to the catheterization laboratory. Swan and Ganz worked in the same department but had quite dissimilar backgrounds and personalities. This article describes their lives and careers, the state of intensive care before and after their catheter was introduced, and the natural life cycle the PA catheter faced as new, less invasive technology arrived to replace it.


Assuntos
Cateterismo de Swan-Ganz/história , Procedimentos Cirúrgicos Cardíacos/história , Cateterismo de Swan-Ganz/efeitos adversos , Cuidados Críticos/história , Tchecoslováquia , Inglaterra , História do Século XIX , História do Século XX , História do Século XXI , Humanos , Los Angeles
13.
Pathog Dis ; 76(7)2018 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-30184175

RESUMO

Bordetella pertussis is a strictly human pathogen causing the respiratory infectious disease called whooping cough or pertussis. B. pertussis adaptation to acellular pertussis vaccine pressure has been repeatedly highlighted, but recent data indicate that adaptation of circulating strains started already in the era of the whole cell pertussis vaccine (wP) use. We sequenced the genomes of five B. pertussis wP vaccine strains isolated in the former Czechoslovakia in the pre-wP (1954-1957) and early wP (1958-1965) eras, when only limited population travel into and out of the country was possible. Four isolates exhibit a similar genome organization and form a distinct phylogenetic cluster with a geographic signature. The fifth strain is rather distinct, both in genome organization and SNP-based phylogeny. Surprisingly, despite isolation of this strain before 1966, its closest sequenced relative appears to be a recent isolate from the US. On the genome content level, the five vaccine strains contained both new and already described regions of difference. One of the new regions contains duplicated genes potentially associated with transport across the membrane. The prevalence of this region in recent isolates indicates that its spread might be associated with selective advantage leading to increased strain fitness.


Assuntos
Bordetella pertussis/genética , Genômica , Vacina contra Coqueluche/genética , Bordetella pertussis/isolamento & purificação , República Tcheca , Tchecoslováquia , Ordem dos Genes , Variação Genética , Humanos , Sequenciamento Completo do Genoma
14.
Parasit Vectors ; 11(1): 447, 2018 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-30071897

RESUMO

BACKGROUND: Amphibian trypanosomes were the first ever described trypanosomatids. Nevertheless, their taxonomy remains entangled because of pleomorphism and high prevalence of mixed infections. Despite the fact that the first species in this group were described in Europe, virtually none of the trypanosomes from European anurans was analyzed using modern molecular methods. METHODS: In this study, we explored the diversity and phylogeny of trypanosomes in true frogs from Europe using light microscopy and molecular methods. RESULTS: A comparison of observed morphotypes with previous descriptions allowed us to reliably identify three Trypanosoma spp., whereas the remaining two strains were considered to represent novel taxa. In all cases, more than one morphotype per blood sample was observed, indicating mixed infections. One hundred and thirty obtained 18S rRNA gene sequences were unambiguously subdivided into five groups, correspondent to the previously recognized or novel taxa of anuran trypanosomes. CONCLUSIONS: In this work we studied European frog trypanosomes. Even with a relatively moderate number of isolates, we were able to find not only three well-known species, but also two apparently new ones. We revealed that previous assignments of multiple isolates from distant geographical localities to one species based on superficial resemblance were unjustified. Our work also demonstrated a high prevalence of mixed trypanosome infections in frogs and proposed a plausible scenario of evolution of the genus Trypanosoma.


Assuntos
Anuros/parasitologia , Filogenia , Trypanosoma/genética , Animais , Anuros/sangue , Clonagem Molecular , Tchecoslováquia , Variação Genética , Reação em Cadeia da Polimerase , RNA de Protozoário/genética , RNA Ribossômico 18S/genética , Especificidade da Espécie , Trypanosoma/fisiologia , Ucrânia
15.
BMC Genomics ; 19(1): 533, 2018 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-30005602

RESUMO

BACKGROUND: Genomic methods can provide extraordinary tools to explore the genetic background of wild species and domestic breeds, optimize breeding practices, monitor and limit the spread of recessive diseases, and discourage illegal crossings. In this study we analysed a panel of 170k Single Nucleotide Polymorphisms with a combination of multivariate, Bayesian and outlier gene approaches to examine the genome-wide diversity and inbreeding levels in a recent wolf x dog cross-breed, the Czechoslovakian Wolfdog, which is becoming increasingly popular across Europe. RESULTS: Pairwise FST values, multivariate and assignment procedures indicated that the Czechoslovakian Wolfdog was significantly differentiated from all the other analysed breeds and also well-distinguished from both parental populations (Carpathian wolves and German Shepherds). Coherently with the low number of founders involved in the breed selection, the individual inbreeding levels calculated from homozygosity regions were relatively high and comparable with those derived from the pedigree data. In contrast, the coefficient of relatedness between individuals estimated from the pedigrees often underestimated the identity-by-descent scores determined using genetic profiles. The timing of the admixture and the effective population size trends estimated from the LD patterns reflected the documented history of the breed. Ancestry reconstruction methods identified more than 300 genes with excess of wolf ancestry compared to random expectations, mainly related to key morphological features, and more than 2000 genes with excess of dog ancestry, playing important roles in lipid metabolism, in the regulation of circadian rhythms, in learning and memory processes, and in sociability, such as the COMT gene, which has been described as a candidate gene for the latter trait in dogs. CONCLUSIONS: In this study we successfully applied genome-wide procedures to reconstruct the history of the Czechoslovakian Wolfdog, assess individual wolf ancestry proportions and, thanks to the availability of a well-annotated reference genome, identify possible candidate genes for wolf-like and dog-like phenotypic traits typical of this breed, including commonly inherited disorders. Moreover, through the identification of ancestry-informative markers, these genomic approaches could provide tools for forensic applications to unmask illegal crossings with wolves and uncontrolled trades of recent and undeclared wolfdog hybrids.


Assuntos
Cães/genética , Genoma , Lobos/genética , Animais , Teorema de Bayes , Catecol O-Metiltransferase/genética , Ritmo Circadiano/genética , Tchecoslováquia , DNA/isolamento & purificação , DNA/metabolismo , Ontologia Genética , Genética Populacional , Hibridização Genética , Desequilíbrio de Ligação , Metabolismo dos Lipídeos/genética , Polimorfismo de Nucleotídeo Único , Análise de Componente Principal
16.
Photosynth Res ; 137(3): 361-375, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29802563

RESUMO

We provide a tribute to George Feher, one of the founding scientists in the use of biophysical techniques to probe photosynthetic complexes, especially the bacterial reaction center. His early life is briefly reviewed followed by a description of the impact of his 30 years of photosynthesis research. We describe his pioneering work in bacterial photosynthesis that helped to provide a detailed picture of the molecular events responsible for light energy capture and the subsequent electron and proton transfer events in photosynthetic organisms. These studies had a profound and lasting impact on our understanding of the molecular mechanisms of photosynthesis. We also include some personal comments from his former students and colleagues.


Assuntos
Fotossíntese , Complexo de Proteína do Fotossistema II/história , Oxigênio Singlete/história , California , Tchecoslováquia , Transporte de Elétrons , História do Século XX , História do Século XXI , Israel
18.
Blood Press ; 27(5): 256-261, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29566565

RESUMO

AIMS: Several papers reported that unattended automated office blood pressure (uAutoOBP) is closely related to daytime ambulatory blood pressure monitoring (ABPM). In the present study, we aim to study uAutoOBP and its relation to 24-hour ABPM and ABPM variability. MATERIAL AND METHODS: Stable treated hypertensive subjects were examined in two Czech academic hypertension centres. uAutoOBP was measured with the BP Tru device; attended BP three times with auscultatory method (AuscOBP) by the physician. ABPM was performed within one week from the clinical visit. RESULTS: Data on 98 subjects aged 67.7 ± 9.3 years with 24-hour ABPM 120.3 ± 10.6/72.7 ± 7.9 mm Hg are reported. uAutoOBP was lower than 24-hour (by -5.2 ± 11.3/-0.5 ± 6.9 mm Hg) and daytime (by -6.7 ± 12.82.4 ± 8.0 mm Hg) ABPM and the individual variability of the difference was very large (up to 30 mm Hg). The correlation coefficients between ABPM and uAutoOBP were similar compared to AuscOBP (p ≥ .17). Variability of uAutoOBP, but not AuscOBP, readings during one clinical visit was related to short-term blood pressure variability of ABPM. The difference between AuscOBP and uAutoOBP was larger in patients with white-coat effect compared to other blood pressure control groups (25.1 ± 7.0 vs. 2.2 ± 10.3 mm Hg; p = .0036). CONCLUSIONS: Our study shows that uAutoOBP is not good predictor of ambulatory blood pressure monitoring, not even of the daytime values. It might, however, indicate short-term blood pressure variability and, when compared with AuscOBP, also detect patients with white-coat effect.


Assuntos
Determinação da Pressão Arterial/métodos , Monitorização Ambulatorial da Pressão Arterial/métodos , Hipertensão do Jaleco Branco/diagnóstico , Adulto , Idoso , Tchecoslováquia , Feminino , Humanos , Hipertensão , Masculino , Hipertensão do Jaleco Branco/fisiopatologia
19.
J Eukaryot Microbiol ; 65(5): 733-741, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29377485

RESUMO

Two events have helped to shape protozoology/protistology as a specific scientific discipline. The first such event was the creation of the Society of Protozoologists in the U.S. in 1947 (and of its Journal of Protozoology, first published in 1954), the second event was the First International Conference on Protozoology, held in 1961 in Prague. The history of the Society of Protozoologists was comprehensively treated by Corliss (1998); the history of the Prague Conference is presented here as reminiscences and personal interpretation of events of the author, who was one of the conference organizers and a member of the organization committee. Special attention is given to the personality and scientific accomplishments of Otto Jírovec, the 1961 conference spiritual father and president. It is concluded that the Prague Conference, while establishing the tradition of protistology meetings, helped protistology to attain its present status as a fundamental science discipline, which discovers and interprets the web of life at one of its, basic, "microbial" levels. Protists literally permeate the earth biosphere and in a way represent the "dark matter" of the living world, still awaiting many discoveries.


Assuntos
Congressos como Assunto/história , Eucariotos , Congressos como Assunto/organização & administração , Tchecoslováquia , Eucariotos/classificação , Eucariotos/genética , Eucariotos/isolamento & purificação , História do Século XX , História do Século XXI , Humanos , Pneumocystis , Toxoplasma/genética , Toxoplasma/fisiologia , Toxoplasmose/história , Toxoplasmose/parasitologia
20.
Eur J Nutr ; 57(2): 513-521, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27757593

RESUMO

PURPOSE: Gestational diabetes mellitus (GDM) represents the most frequent metabolic disorder in pregnancy. Since dietary intake plays an important role in obesity and type 2 diabetes development, it is likely to be for the susceptibility to GDM too. Food preferences, driving partly the diet composition, are changing during pregnancy. Taste and genetic variability in taste receptors is an important factor in determining food preferences. Aims of our study were (1) to characterize dietary habits of pregnant women and to find possible differences in food preferences between healthy pregnant women and those with GDM and (2) to ascertain possible association of several single nucleotide polymorphisms (SNPs) in taste receptor (TR) genes with GDM. METHODS: A total of 363 pregnant women (293 with GDM and 70 with physiologic pregnancy) were included in the study. Dietary pattern spanning the period of approx. 6 months preceding the time of GDM screening was assessed using a semi-quantitative food frequency questionnaire. A total of five SNPs in TR genes were selected for genotyping based on their functionality or previous associations. RESULTS: Women with GDM exhibited significantly more frequent meat consumption (esp. poultry, pork and smoked meat), dairy products and sweet beverages consumption. The legumes consumption was found to be inversely correlated with fasting glycaemia (P = 0.007, Spearman). CC genotype in TAS2R9 gene (SNP rs3741845) was significantly associated with GDM (P = 0.0087, Chi-square test). CONCLUSIONS: Our study showed differences in dietary intake of selected food items between healthy pregnant women and those with GDM and genetic association of bitter taste receptor allele with GDM.


Assuntos
Diabetes Gestacional/genética , Preferências Alimentares , Cooperação do Paciente , Polimorfismo de Nucleotídeo Único , Fenômenos Fisiológicos da Nutrição Pré-Natal , Receptores Acoplados a Proteínas-G/genética , Adulto , Estudos de Casos e Controles , Tchecoslováquia , Diabetes Gestacional/diagnóstico , Diabetes Gestacional/etiologia , Diabetes Gestacional/prevenção & controle , Dieta Ocidental/efeitos adversos , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Gravidez , Diagnóstico Pré-Natal , Autorrelato
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