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1.
Bull Exp Biol Med ; 167(1): 47-49, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31177448

RESUMO

We studied the influence of synthetic indolicidin analogues on the development of acute periodontitis. The corrective effect was found in indolicidin analogues Nos. 7 and 8; it manifested in a decrease in the edema of gingival epithelium and lamina propria, a decrease in the relative area of inflammatory infiltrates, and a significant increase in the relative area of normal connective tissue. These changes were revealed as soon as on day 14 and were most pronounced in 21 days after the removal of the ligature. Indolicidin analogues Nos. 7 and 8 demonstrated similar effectiveness on the model of acute periodontitis.


Assuntos
Peptídeos Catiônicos Antimicrobianos/uso terapêutico , Periodontite/tratamento farmacológico , Animais , Peptídeos Catiônicos Antimicrobianos/química , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/metabolismo , Gengiva/citologia , Gengiva/metabolismo , Membrana Mucosa/efeitos dos fármacos , Membrana Mucosa/metabolismo , Ratos Wistar
2.
Aquat Toxicol ; 213: 105218, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31203168

RESUMO

The toxic effects of different atrazine concentrations on tadpoles and adult male African clawed frogs (Xenopus laevis) were assessed in a controlled laboratory environment following 90 days' exposure. The aim was to elucidate the danger of atrazine exposure on the cardiac tissue relative to its critical function of rhythmic contractility, fundamental for optimal blood circulation and homeostasis. Tadpoles and adult frogs were exposed to 0 µg/L (control), 0.01 µg L-1, 200 µg L-1 and 500 µg L-1 concentrations of atrazine for 90 days. Mortality was concenration-dependent and significantly increased in juvenile group (77%, 43%, 23% and 0 respectively for 500 µg L-1, 200 µg L-1, 0.01 µg L-1, and control group). While the mean juvenile heart area decreased concentration-dependently, adult frog mean heart area significantly increased in the 200 µg L-1 group only and mean heart weight change was variable across all exposure levels. Light microscopy of hematoxylin and eosin (H&E) and Mallory-Heidenhain rapid one-step staining techniques on cardiac tissue sections of the juvenile and adult frogs revealed shrinkage of cardiac muscle cells into thin wavy myocytes. Additionally, disorganized branching of muscle fibres with reduced striations were observed in 0.01 µg L-1 and 200 µg L-1 but hypertrophied myocytes, thickened intensely staining myofibrils in the 500 µg L-1 group in juvenile and adult frogs. Significant increase in the mean percentage area of connective tissue in all the treated groups (p < 0.036) were also recorded. Immunohistochemistry analysis showed decreased eNOS localization in cardiac tissue in 200 µg L-1 and 500 µg L-1 of both juvenile and adult group, suggestive of decreased cardiac contractility due to atrazine exposure. The results indicate that environmentally relevant atrazine concentrations cause significant mortality in tadpoles while concentrations ≥200 µg L-1 adversely affect cardiac muscle morphology and may induce functional perturbations in cardiac tissue contractility and consequent dysfunction which generally may have an adverse impact on their survival and longevity.


Assuntos
Atrazina/toxicidade , Cardiotoxicidade/patologia , Poluentes Químicos da Água/toxicidade , Xenopus laevis/fisiologia , Animais , Tecido Conjuntivo/efeitos dos fármacos , Coração/efeitos dos fármacos , Larva/efeitos dos fármacos , Masculino , Óxido Nítrico Sintase Tipo III/metabolismo , Tamanho do Órgão/efeitos dos fármacos , Análise de Sobrevida
3.
J Mech Behav Biomed Mater ; 84: 208-216, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29793158

RESUMO

Botulinum toxin type A (BTX-A) effects on the mechanics of non-injected antagonistic muscles are unknown. The aim was to test the following hypotheses in a rat model: BTX-A injected into gastrocnemius medialis (GM) and lateralis (GL) (1) decreases forces of the antagonistic tibialis anterior (TA) and extensor digitorum longus (EDL), (2) reduces length range of force exertion and (3) increases passive forces of the TA, and (4) changes inter-antagonistic and inter-synergistic epimuscular myofascial force transmission (EMFT). Two groups of Wistar rats were tested: BTX (0.1 units of BTX-A were injected to the GM and GL, each) and Control (saline injected). Five-days post, TA, EDL, GM-GL, and soleus distal and EDL proximal isometric forces were measured after TA lengthening. BTX-A exposure caused forces of all muscles to decrease significantly. TA and EDL active force drops (maximally by 37.3%) show inter-compartmental spread. Length range of force exertion of the TA did not change, but its passive force increased significantly (by 25%). The percentages of intramuscular connective tissue content of the TA and EDL was higher (BTX: 20.0 ±â€¯4.9% and 19.3 ±â€¯4.1% vs. control: 13.1 ±â€¯5.4% and 14.5 ±â€¯4.0%, respectively). Calf muscles' forces were not affected by TA length changes for both groups indicating lacking inter-antagonistic EMFT. However, BTX-A altered EDL proximo-distal force differences hence, inter-synergistic EMFT. A major novel finding is that BTX-A affects mechanics of non-injected antagonistic muscles in test conditions involving only limited EMFT. The effects indicating a stiffer muscle with no length range increase contradict some treatment aims, which require clinical testing.


Assuntos
Toxinas Botulínicas Tipo A/farmacologia , Fenômenos Mecânicos/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Animais , Fenômenos Biomecânicos/efeitos dos fármacos , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/metabolismo , Masculino , Músculo Esquelético/fisiologia , Ratos , Ratos Wistar
4.
PLoS One ; 13(4): e0193456, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29630675

RESUMO

To determine the effect of mechanical stretching load and the efficacy of postmenopausal estrogen therapy (ET) on pelvic organ prolapse (POP), vaginal fibroblasts isolated from postmenopausal women with or without POP were subjected to 0.1-Hz uniaxial cyclic mechanical stretching (CS) with 10% elongation and 10-8 M 17-ß-estradiol (E2) treatment. We investigated the morphological characteristics of extracellular polymers using scanning electron microscopy (SEM) and monitored the mRNA expression of type I collagen (COL I) and type III collagen (COL III) as well as the small leucine-rich proteoglycan (SLRP) family members decorin (DCN), biglycan (BGN), fibromodulin (FMO), and lumican (LUM), using real-time quantitative polymerase chain reaction (RT-PCR). Using SEM, certain viscoelastic polymers were found to be randomly distributed among fibroblasts, which for normal fibroblasts formed clusters of plum flower-like patterns under static-culture conditions and resembled stretched strips when stretched in culture, whereas polymers among POP fibroblasts resembled stretched strips under static-cultured conditions and presented broken networks when stretched in culture. RT-PCR revealed that COL I, DCN, BGN, FMO, and LUM mRNA expression was significantly higher in POP than in normal fibroblasts under static-culture condition. Following CS, COL I and BGN mRNA expression was significantly up-regulated in normal fibroblasts, and DCN and FMO mRNA expression was down-regulated in POP fibroblasts. Following concomitant CS and E2 treatment, significantly elevated COL I and DCN mRNA expression was observed in normal fibroblasts, and significantly elevated COL I and BGN mRNA expression was observed in POP fibroblasts. COL III mRNA expression was not significantly different between the POP and normal group, and CS did not significantly affect expression in either group, though COL III was down-regulated in normal fibroblasts concomitantly treated with E2 and CS. We conclude that the morphological distribution of extracellular polymers in POP fibroblasts exhibited higher sensitivity and lower tolerance to stretching loads than do normal fibroblasts. These mechanical properties were further reflected in the transcription of COL I. Defects in the compensatory function of BGN for DCN and LUM for FMO exist in POP fibroblasts, which further affect the structure and function of COL I in response to stretching load, ultimately resulting in abnormal reconstruction of pelvic supportive connective tissues and the occurrence of POP. ET can maintain stretching-induced elevations in COL I and DCN transcription in healthy women and improve stretching-induced COL I, DCN, BGN, and FMO transcriptional changes in POP women to prevent and improve POP. Only down-regulated COL III transcription was observed upon concomitant CS and E2 treatment in normal fibroblasts, which suggests that the tensile strength, not the elasticity, of the supportive connective tissues is damaged in POP and that the higher tensile strength induced by ET in healthy fibroblasts prevents POP. These findings confirm the role of higher sensitivity and lower tolerance to mechanical stretching in the pathogenesis of POP and further provide evidence supporting the use of ET to prevent and inhibit POP in postmenopausal women.


Assuntos
Colágeno/metabolismo , Fibroblastos/metabolismo , Prolapso de Órgão Pélvico/metabolismo , Proteoglicanos Pequenos Ricos em Leucina/metabolismo , Estresse Mecânico , Vagina/metabolismo , Idoso , Colágeno/genética , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/metabolismo , Estradiol/farmacologia , Feminino , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Humanos , Pessoa de Meia-Idade , Prolapso de Órgão Pélvico/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Proteoglicanos Pequenos Ricos em Leucina/genética , Vagina/citologia , Vagina/efeitos dos fármacos
5.
Clin Implant Dent Relat Res ; 20(3): 294-299, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29508553

RESUMO

BACKGROUND: Cells, scaffolds, and growth factors are the key components in bone tissue engineering. Scaffold composition, topography, and architecture influence the amount of regenerated bone in the implantation site. The aims of the study were to compare viability and proliferation of mesenchymal stem cells (MSCs) seeded onto two commercial xenografts: Bio-Oss (BO) and bioactive bone bovine (BB). Next, these materials were compared for histomorphometric bone formation in a socket preservation model in rats. MATERIALS AND METHODS: MSCs were seeded onto monolayers of BO or BB granules. Cell viability and proliferation were evaluated after incubation of 0, 2, 20, and 48 h. A total of 24 Sprague Dawley rats underwent unilateral extraction of maxillary molars. Rats were randomly divided into three groups: natural healing (nongrafted socket) or socket preservation with either BO or BB. Rats were sacrificed after 8 weeks, and histomorphometric analysis was done to evaluate bone formation and residual scaffold at the extraction site. RESULTS: Differences in the metabolic activity of MSCs that were seeded onto BO or BB was observed at 2 h after seeding: the metabolic activity was elevated compared to baseline in the BB (P = .046) and not changed in the BO wells (P = .84). After 20 h, the metabolic activity of MSCs seeded onto BO was decreasing (P = .005), while cell viability was not changed in the BB group (P = .356). Intergroup comparison revealed higher metabolic activity of MSCs seeded on BB after 48 h compared with BO (P = .016). The in vivo results demonstrated differences in socket healing between the groups: percentage of new bone was higher in the BB compared to BO group (39.1 ± 14.3 vs. 23.7 ± 10.8%, respectively, P = .096). Connective tissue portion was higher in the BO group compared with BB (73.7 ± 11.1 vs. 49.6 ± 13.7%, respectively, P = .018). Residual grafting martial was higher in the BB (11.34 ± 4.18 vs. 2.62 ± 1.23%, P = .011). CONCLUSIONS: The results of this study demonstrating higher vitality and proliferation of MSCs seeded onto BB. Furthermore, following ridge preservation, higher percentage of new bone and lower residual scaffold were found in the BB compared with BO. This enhanced regenerative response might be the result of an enhancement of metabolic activity in cells attached to it. Further research will be needed to understand the precise mechanism.


Assuntos
Aumento do Rebordo Alveolar/métodos , Materiais Biocompatíveis/farmacologia , Regeneração Óssea , Transplante Ósseo , Xenoenxertos/patologia , Xenoenxertos/fisiologia , Animais , Regeneração Óssea/efeitos dos fármacos , Transplante Ósseo/métodos , Bovinos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/patologia , Tecido Conjuntivo/fisiologia , Teste de Materiais , Maxila/diagnóstico por imagem , Maxila/patologia , Maxila/cirurgia , Minerais/farmacologia , Modelos Animais , Dente Molar/diagnóstico por imagem , Dente Molar/cirurgia , Osteogênese/efeitos dos fármacos , Osteogênese/fisiologia , Ratos , Ratos Sprague-Dawley , Engenharia Tecidual/métodos , Tecidos Suporte , Extração Dentária , Alvéolo Dental/diagnóstico por imagem , Alvéolo Dental/patologia , Alvéolo Dental/transplante , Cicatrização/fisiologia
6.
Metallomics ; 10(3): 463-473, 2018 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-29485154

RESUMO

Coal mining is one of the economic activities with the greatest impact on environmental quality. At all stages contaminants are released as particulates such as coal dust. The first aim of this study was to obtain an aqueous coal dust extract and characterize its composition in terms of trace elements by ICP-MS. In addition, the developmental toxicity of the aqueous coal extract was evaluated using zebrafish (Danio rerio) after exposure to different concentrations (0-1000 ppm; µg mL-1) to establish acute toxicity, morphology and transcriptome changes. Trace elements within the aqueous coal dust extract present at the highest concentrations (>10 ppb) included Sr, Zn, Ba, As, Cu and Se. In addition, Cd and Pb were found in lower concentrations. No significant difference in mortality was observed (p > 0.05), but a delay in hatching was found at 0.1 and 1000 ppm (p < 0.05). No significant differences in morphological characteristics were observed in any of the treatment groups (p > 0.05). Transcriptomic results of zebrafish larvae revealed alterations in 77, 61 and 1376 genes in the 1, 10, and 100 ppm groups, respectively. Gene ontology analysis identified gene alterations associated with the development and function of connective tissue and the hematological system, as well as pathways associated with apoptosis, the cell cycle, transcription, and oxidative stress including the MAPK signaling pathway. In addition, altered genes were associated with cancer; connective tissue, muscular, and skeletal disorders; and immunological and inflammatory diseases. Overall, this is the first study to characterize gene expression alterations in response to developmental exposure to aqueous coal dust residue from coal mining with transcriptome results signifying functions and systems to target in future studies.


Assuntos
Carvão Mineral/toxicidade , Tecido Conjuntivo/patologia , Poeira/análise , Regulação da Expressão Gênica no Desenvolvimento , Doenças Hematológicas/patologia , Doenças do Sistema Imunitário/patologia , Inflamação/patologia , Peixe-Zebra/crescimento & desenvolvimento , Animais , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/metabolismo , Poluentes Ambientais/toxicidade , Doenças Hematológicas/induzido quimicamente , Doenças Hematológicas/genética , Doenças do Sistema Imunitário/induzido quimicamente , Doenças do Sistema Imunitário/genética , Inflamação/induzido quimicamente , Inflamação/genética , Transcriptoma , Peixe-Zebra/genética
7.
Toxicol In Vitro ; 48: 276-285, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29409908

RESUMO

The aim of the present study was to evaluate the cytotoxicity of pristine graphene monolayer and its utility as a scaffold for murine fibroblast L929 cell line. Cell viability, morphology, cytoskeleton architecture (microfilaments and microtubules), cell adhesion and migration into the scratch-wound area were determined using pristine graphene-coated microscopic slides. We found that fibroblasts cultured on pristine graphene monolayer exhibited changes in cell attachment, motility and cytoskeleton organization. Graphene was found to have no cytotoxicity on L929 fibroblasts and increased cell adhesion and proliferation within 24 h of culture. The area of cells growing on graphene was comparable to the area of fibroblasts cultured on glass. Migration of cells on the surface of graphene substrate appeared to be more regular in comparison to uncoated glass surface, however in both control (glass) and experimental (graphene) groups the scratch wound was closed after 48 h of culture. Taken together, our results indicate that pristine graphene monolayer is non-toxic for murine subcutaneous connective tissue fibroblasts and could be beneficial for recovery of damaged tissues after injury. These studies could be helpful in evaluating biocompatibility of graphene, which still remains ambiguous.


Assuntos
Materiais Biocompatíveis/toxicidade , Fibroblastos/efeitos dos fármacos , Grafite/toxicidade , Teste de Materiais , Citoesqueleto de Actina/metabolismo , Animais , Adesão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Tecido Conjuntivo/efeitos dos fármacos , Citoesqueleto/efeitos dos fármacos , Citoesqueleto/ultraestrutura , Camundongos , Camundongos Endogâmicos C3H , Tecidos Suporte , Cicatrização/efeitos dos fármacos
8.
ACS Nano ; 12(2): 1482-1490, 2018 02 27.
Artigo em Inglês | MEDLINE | ID: mdl-29365250

RESUMO

Surgical blades are common medical tools. However, blades cannot distinguish between healthy and diseased tissue, thereby creating unnecessary damage, lengthening recovery, and increasing pain. We propose that surgical procedures can rely on natural tissue remodeling tools-enzymes, which are the same tools our body uses to repair itself. Through a combination of nanotechnology and a controllably activated proteolytic enzyme, we performed a targeted surgical task in the oral cavity. More specifically, we engineered nanoparticles that contain collagenase in a deactivated form. Once placed at the surgical site, collagenase was released at a therapeutic concentration and activated by calcium, its biological cofactor that is naturally present in the tissue. Enhanced periodontal remodeling was recorded due to enzymatic cleavage of the supracrestal collagen fibers that connect the teeth to the underlying bone. When positioned in their new orientation, natural tissue repair mechanisms supported soft and hard tissue recovery and reduced tooth relapse. Through the combination of nanotechnology and proteolytic enzymes, localized surgical procedures can now be less invasive.


Assuntos
Colágeno/metabolismo , Colagenases/administração & dosagem , Colagenases/farmacologia , Tecido Conjuntivo/efeitos dos fármacos , Lipossomos/química , Nanopartículas/química , Animais , Colagenases/farmacocinética , Tecido Conjuntivo/metabolismo , Sistemas de Liberação de Medicamentos/métodos , Enzimas Imobilizadas/administração & dosagem , Enzimas Imobilizadas/farmacocinética , Enzimas Imobilizadas/farmacologia , Masculino , Boca/efeitos dos fármacos , Boca/metabolismo , Boca/cirurgia , Nanotecnologia/métodos , Proteólise/efeitos dos fármacos , Ratos Wistar
9.
J Biochem Mol Toxicol ; 32(2)2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29315975

RESUMO

Rutin, naturally occurring flavonoid, has reported to cover interesting multiple pharmacological properties. This study evaluated rutin or/and meloxicam effects in paw inflammation induced by formalin in mice. Mice were divided into four groups: I-Formalin group, II-Rutin 60 mg/kg (p.o.), III-Meloxicam 10 mg/kg (p.o.), plus IV-Combined rutin and meloxicam. Therapies were administered once a day for 7 days. The curative effects were assessed on inflammatory, oxidative stress, and apoptosis. Both rutin and/or meloxicam induced marked improvement in paw licking time on the 1st day and by combined treatment only on the 3rd day as well reduction in paw edema% on the 3rd day. Moreover, noticeable progress in liver malondialdehyde content, superoxide dismutase, and sorbitol dehydrogenase activities as well decline in paw interleukin-1ß level and extent of apoptosis. The results spot light on the good influence of combined rutin and meloxicam in formalin-induced mice paw inflammation to a better extent than either rutin or meloxicam lonely.


Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Celulite (Flegmão)/tratamento farmacológico , Tecido Conjuntivo/efeitos dos fármacos , Modelos Animais de Doenças , Fígado/efeitos dos fármacos , Rutina/uso terapêutico , Tiazinas/uso terapêutico , Tiazóis/uso terapêutico , Dor Aguda/etiologia , Dor Aguda/imunologia , Dor Aguda/prevenção & controle , Analgésicos não Entorpecentes/uso terapêutico , Animais , Anti-Inflamatórios não Esteroides/efeitos adversos , Antioxidantes/efeitos adversos , Antioxidantes/uso terapêutico , Apoptose/efeitos dos fármacos , Comportamento Animal/efeitos dos fármacos , Celulite (Flegmão)/metabolismo , Celulite (Flegmão)/patologia , Celulite (Flegmão)/fisiopatologia , Tecido Conjuntivo/imunologia , Tecido Conjuntivo/metabolismo , Tecido Conjuntivo/patologia , Quimioterapia Combinada , Edema/etiologia , Edema/imunologia , Edema/prevenção & controle , L-Iditol 2-Desidrogenase/metabolismo , Fígado/imunologia , Fígado/metabolismo , Fígado/patologia , Masculino , Meloxicam , Camundongos , Tamanho do Órgão/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Distribuição Aleatória , Rutina/efeitos adversos , Superóxido Dismutase/metabolismo , Tiazinas/efeitos adversos , Tiazóis/efeitos adversos
10.
Br J Dermatol ; 178(5): 1011-1019, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29086923

RESUMO

There is growing evidence that botulinum neurotoxins (BoNTs) exhibit biological effects on various human cell types with a host of associated clinical implications. This review aims to provide an update on the non-neuronal and nonmuscular effects of botulinum toxin. We critically analysed recent reports on the structure and function of cellular signalling systems subserving biological effects of BoNTs. The BoNT receptors and intracellular targets are not unique for neurotransmission. They have been found in both neuronal and non-neuronal cells, but there are differences in how BoNT binds to, and acts on, neuronal vs. non-neuronal cells. The non-neuronal cells that express one or more BoNT/A-binding proteins, and/or cleavage target synaptosomal-associated protein 25, include: epidermal keratinocytes; mesenchymal stem cells from subcutaneous adipose; nasal mucosal cells; urothelial cells; intestinal, prostate and alveolar epithelial cells; breast cell lines; neutrophils; and macrophages. Serotype BoNT/A can also elicit specific biological effects in dermal fibroblasts, sebocytes and vascular endothelial cells. Nontraditional applications of BoNT have been reported for the treatment of the following dermatological conditions: hyperhidrosis, Hailey-Hailey disease, Darier disease, inversed psoriasis, aquagenic palmoplantar keratoderma, pachyonychia congenita, multiple eccrine hydrocystomas, eccrine angiomatous hamartoma, eccrine sweat gland naevi, congenital eccrine naevus, Raynaud phenomenon and cutaneous leiomyomas. Experimental studies have demonstrated the ability of BoNT/A to protect skin flaps, facilitate wound healing, decrease thickness of hypertrophic scars, produce an anti-ageing effect, improve a mouse model of psoriasiform dermatitis, and have also revealed extracutaneous effects of BoNT arising from its anti-inflammatory and anticancer properties. BoNTs have a much wider range of applications than originally understood, and the individual cellular responses to the cholinergic impacts of BoNTs could provide fertile ground for future studies.


Assuntos
Toxinas Botulínicas Tipo A/farmacologia , Fármacos Neuromusculares/farmacologia , Neurotoxinas/farmacologia , Dermatopatias/tratamento farmacológico , Animais , Anti-Inflamatórios/farmacologia , Antineoplásicos/farmacologia , Células Cultivadas , Tecido Conjuntivo/efeitos dos fármacos , Cosméticos/farmacologia , Modelos Animais de Doenças , Humanos , Coelhos , Ratos , Pele/irrigação sanguínea , Retalhos Cirúrgicos/irrigação sanguínea
11.
J Periodontal Res ; 53(2): 222-231, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29063626

RESUMO

BACKGROUND AND OBJECTIVE: Nowadays, most designs for the transmucosal surface of implants are machined-smooth. However, connective tissue adhered to the smooth surface of an implant has poor mechanical resistance, which can render separation of tissue from the implant interface and induce epithelial downgrowth. Modification of the transmucosal surface of implants, which can help form a good seal of connective tissue, is therefore desired. We hypothesized that anodic oxidation (AO) and polydopamine (PD) deposition could be used to enhance the attachment between an implant and peri-implant connective tissue. We tested this hypothesis in the mandibles of Beagle dogs. MATERIAL AND METHODS: AO and PD were used to modify the transmucosal region of transmucosal implants (implant neck). The surface microstructure, surface roughness and elemental composition were investigated in vitro. L929 mouse fibroblasts were cultured to test the effect of PD on cell adhesion. Six Beagle dogs were used for the in vivo experiment (n = 6 dogs per group). Three months after building the edentulous animal model, four groups of implants (control, AO, PD and AO + PD) were inserted. After 4 months of healing, samples were harvested for histometric analyses. RESULTS: The surfaces of anodized implant necks were overlaid with densely distributed pores, 2-7 µm in size. On the PD-modified surfaces, N1s, the chemical bond of nitrogen in PD, was detected using X-ray photoelectron spectroscopy. L929 developed pseudopods more quickly on the PD-modified surfaces than on the surfaces of the control group. The in vivo experiment showed a longer connective tissue seal and a more coronally located peri-implant soft-tissue attachment in the AO + PD group than in the control group (P < .05). CONCLUSION: The modification of AO + PD on the implant neck yielded better attachment between the implant and peri-implant connective tissue.


Assuntos
Tecido Conjuntivo/efeitos dos fármacos , Implantes Dentários , Planejamento de Prótese Dentária , Inserção Epitelial/efeitos dos fármacos , Indóis/farmacologia , Polímeros/farmacologia , Animais , Adesão Celular/efeitos dos fármacos , Materiais Revestidos Biocompatíveis , Tecido Conjuntivo/patologia , Implantação Dentária Endo-Óssea , Cães , Inserção Epitelial/patologia , Fibroblastos/efeitos dos fármacos , Mandíbula , Camundongos , Modelos Animais , Osseointegração/efeitos dos fármacos , Oxirredução , Propriedades de Superfície , Fatores de Tempo , Titânio
12.
Int Endod J ; 51(6): 641-648, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29143348

RESUMO

AIM: To investigate whether hypertension affects mineralization associated with white and grey mineral trioxide aggregate (MTA Angelus® ) implanted subcutaneously into rats by assaying osteoblastic biomarkers. METHODOLOGY: Polyethylene tubes containing grey MTA Angelus® , white MTA Angelus® , intermediate restorative material (IRM; positive control) or an empty tube (negative control) were implanted into the dorsal connective tissue of spontaneous hypertensive (n = 12) and Wistar (normotensive; n = 10) rats. Half of the rats in each group were killed after 7 days, and the remaining after 30 days. Tubes with surrounding tissue were removed, and immunostaining was performed to detect RUNX-2, OPN and OCN proteins. The normality of data was analysed using the Shapiro-Wilk test. Comparison of two independent groups was performed using the Mann-Whitney U-test, to detect a significant difference. A post hoc test accounting for multiple comparisons was performed following Tukey's test (P < 0.05). RESULTS: Under hypertensive conditions after 30 days, both MTA materials were associated with immunolabelling for RUNX-2 from low to moderate, which was less than that observed at normal blood pressure and the 7-day groups (P < 0.05). The expression of OPN and OCN proteins under both MTA conditions was considered low after both 7 and 30 days for the hypertensive condition, and was less than that in animals with normal blood pressure after 30 days (P < 0.05). No immunostaining for any biomarkers in the control and IRM groups was observed (P < 0.05). CONCLUSION: Hypertension decreased the immunostaining of RUNX-2, OPN and OCN biomarkers in response to MTA. Thus, hypertension can jeopardize the mineralization ability of MTA and may have a negative impact on endodontic treatment outcomes.


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Proteínas do Citoesqueleto/metabolismo , Proteínas Ativadoras de GTPase/metabolismo , Hipertensão/metabolismo , Proteínas Nucleares/metabolismo , Osteocalcina/metabolismo , Óxidos/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Silicatos/farmacologia , Animais , Materiais Biocompatíveis/farmacologia , Biomarcadores/metabolismo , Tecido Conjuntivo/efeitos dos fármacos , Combinação de Medicamentos , Ratos , Ratos Wistar
13.
Einstein (Sao Paulo) ; 15(2): 186-191, 2017.
Artigo em Inglês, Português | MEDLINE | ID: mdl-28767917

RESUMO

Objective: To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods: Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury. At the end of the experiment, the tibialis anterior and soleus muscles were dissected and processed for light microscopy and submitted to morphological analysis. Results: The nerve compression promoted morphological changes, typical of denervation, and the treatment with vanillin was responsible for different responses in the studied muscles. For the tibialis anterior, there was an increase in the number of satellite cells, central nuclei and fiber atrophy, as well as fascicular disorganization. In the soleus, only increased vascularization was observed, with no exacerbation of the morphological alterations in the fibers. Conclusion: The treatment with vanillin promoted increase in intramuscular vascularization for the muscles studied, with pro-inflammatory potential for tibialis anterior, but not for soleus muscle. Objetivo: Avaliar a ação da vanilina (Vanilla planifolia) sobre a morfologia dos músculos tibial anterior e sóleo após lesão nervosa periférica. Métodos: Ratos Wistar foram divididos em quatro grupos, com sete animais cada, sendo Grupo Controle, Grupo Vanilina, Grupo Lesão e Grupo Lesão + Vanilina. Os animais dos Grupos Lesão e Grupo Lesão + Vanilina foram submetidos à lesão nervosa por meio da compressão do nervo isquiático, e os Grupos Vanilina e Grupo Lesão + Vanilina foram tratados diariamente com doses orais de vanilina (150mg/kg) do 3o ao 21o dia após a indução da lesão nervosa. Ao término do experimento, os músculos tibial anterior e sóleo foram dissecados e seguiram o processamento de rotina em microscopia de luz, para posterior análise morfológica. Resultados: A compressão nervosa promoveu alterações morfológicas características de denervação, sendo que o tratamento com vanilina foi responsável por respostas distintas nos músculos estudados. Para o tibial anterior, houve aumento do número de células satélites, núcleos centrais e atrofia das fibras, bem como desorganização fascicular. Já no sóleo, houve apenas aumento da vascularização, sem exacerbação das alterações morfológicas nas fibras. Conclusão: O tratamento com vanilina promoveu o aumento da vascularização intramuscular para os músculos estudados, com potencial pró-inflamatório para o tibial anterior, o que não ocorreu no músculo sóleo.


Assuntos
Anti-Inflamatórios/farmacologia , Benzaldeídos/farmacologia , Tecido Conjuntivo/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Neuropatia Ciática/patologia , Animais , Tecido Conjuntivo/patologia , Humanos , Masculino , Modelos Animais , Fibras Musculares Esqueléticas/efeitos dos fármacos , Músculo Esquelético/patologia , Distribuição Aleatória , Ratos Wistar , Neuropatia Ciática/reabilitação
14.
Int J Mol Sci ; 18(8)2017 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-28829377

RESUMO

Cadmium (Cd) is one of the most harmful metals, being toxic to most animal species, including marine invertebrates. Among marine gastropods, the periwinkle (Littorina littorea) in particular can accumulate high amounts of Cd in its midgut gland. In this organ, the metal can elicit extensive cytological and tissue-specific alterations that may reach, depending on the intensity of Cd exposure, from reversible lesions to pathological cellular disruptions. At the same time, Littorina littorea expresses a Cd-specific metallothionein (MT) that, due to its molecular features, expectedly exerts a protective function against the adverse intracellular effects of this metal. The aim of the present study was, therefore, to assess the time course of MT induction in the periwinkle's midgut gland on the one hand, and cellular and tissue-specific alterations in the digestive organ complex (midgut gland and digestive tract) on the other, upon exposure to sub-lethal Cd concentrations (0.25 and 1 mg Cd/L) over 21 days. Depending on the Cd concentrations applied, the beginning of alterations of the assessed parameters followed distinct concentration-dependent and time-dependent patterns, where the timeframe for the onset of the different response reactions became narrower at higher Cd concentrations compared to lower exposure concentrations.


Assuntos
Cádmio/administração & dosagem , Cádmio/toxicidade , Fenômenos Fisiológicos do Sistema Digestório/efeitos dos fármacos , Gastrópodes/efeitos dos fármacos , Gastrópodes/fisiologia , Sequência de Aminoácidos , Animais , Cálcio/metabolismo , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/metabolismo , Tecido Conjuntivo/patologia , Exposição Ambiental , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Metalotioneína/química , Metalotioneína/genética , Metalotioneína/metabolismo , Polimorfismo de Nucleotídeo Único
15.
Einstein (Säo Paulo) ; 15(2): 186-191, Apr.-June 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-891383

RESUMO

ABSTRACT Objective To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury. At the end of the experiment, the tibialis anterior and soleus muscles were dissected and processed for light microscopy and submitted to morphological analysis. Results The nerve compression promoted morphological changes, typical of denervation, and the treatment with vanillin was responsible for different responses in the studied muscles. For the tibialis anterior, there was an increase in the number of satellite cells, central nuclei and fiber atrophy, as well as fascicular disorganization. In the soleus, only increased vascularization was observed, with no exacerbation of the morphological alterations in the fibers. Conclusion The treatment with vanillin promoted increase in intramuscular vascularization for the muscles studied, with pro-inflammatory potential for tibialis anterior, but not for soleus muscle.


RESUMO Objetivo Avaliar a ação da vanilina (Vanilla planifolia) sobre a morfologia dos músculos tibial anterior e sóleo após lesão nervosa periférica. Métodos Ratos Wistar foram divididos em quatro grupos, com sete animais cada, sendo Grupo Controle, Grupo Vanilina, Grupo Lesão e Grupo Lesão + Vanilina. Os animais dos Grupos Lesão e Grupo Lesão + Vanilina foram submetidos à lesão nervosa por meio da compressão do nervo isquiático, e os Grupos Vanilina e Grupo Lesão + Vanilina foram tratados diariamente com doses orais de vanilina (150mg/kg) do 3o ao 21o dia após a indução da lesão nervosa. Ao término do experimento, os músculos tibial anterior e sóleo foram dissecados e seguiram o processamento de rotina em microscopia de luz, para posterior análise morfológica. Resultados A compressão nervosa promoveu alterações morfológicas características de denervação, sendo que o tratamento com vanilina foi responsável por respostas distintas nos músculos estudados. Para o tibial anterior, houve aumento do número de células satélites, núcleos centrais e atrofia das fibras, bem como desorganização fascicular. Já no sóleo, houve apenas aumento da vascularização, sem exacerbação das alterações morfológicas nas fibras. Conclusão O tratamento com vanilina promoveu o aumento da vascularização intramuscular para os músculos estudados, com potencial pró-inflamatório para o tibial anterior, o que não ocorreu no músculo sóleo.


Assuntos
Humanos , Animais , Masculino , Benzaldeídos/farmacologia , Músculo Esquelético/efeitos dos fármacos , Tecido Conjuntivo/efeitos dos fármacos , Neuropatia Ciática/patologia , Anti-Inflamatórios/farmacologia , Distribuição Aleatória , Ratos Wistar , Músculo Esquelético/patologia , Fibras Musculares Esqueléticas/efeitos dos fármacos , Tecido Conjuntivo/patologia , Neuropatia Ciática/reabilitação , Modelos Animais
16.
Menopause ; 24(10): 1185-1189, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28538602

RESUMO

OBJECTIVE: To determine the effect of hormone therapy (HT) use on pelvic organ support. METHODS: A retrospective observational study involving postmenopausal women with pelvic floor dysfunction attending a tertiary urogynecology center between January 2012 and March 2015. All underwent a clinical examination including International Continence Society Pelvic Organ Prolapse Quantification and 4D translabial ultrasound imaging. Information on current or former use of systemic HT and current local estrogen use was collected. Main outcome measure was pelvic organ support. RESULTS: One thousand four hundred forty-three women were seen during the study period. On univariate analysis, current HT was significantly associated with sonographically determined descent of the rectal ampulla (ß [95% confidence interval] 3.4 mm [0.4-6.5], P = 0.03) and Gh + Pb (-0.45 mm [-0.8 to -0.1], P = 0.005). Past HT use, duration of HT use, or current vaginal estrogen use was not associated with pelvic organ support. On multivariate analysis controlling for age, parity, body mass index, history of forceps delivery, and avulsion, the association between current HT on the one hand and Gh + Pb as well as increased descent of the rectal ampulla on ultrasound, remained significant (P = 0.008 and P = 0.012, respectively). CONCLUSION: HT may have a minor negative effect on pelvic organ support; however, the effect is likely too small to be clinically relevant.


Assuntos
Estrogênios/efeitos adversos , Terapia de Reposição Hormonal/efeitos adversos , Prolapso de Órgão Pélvico/fisiopatologia , Pós-Menopausa , Adulto , Idoso , Idoso de 80 Anos ou mais , Tecido Conjuntivo/efeitos dos fármacos , Feminino , Humanos , Pessoa de Meia-Idade , Diafragma da Pelve/diagnóstico por imagem , Diafragma da Pelve/fisiopatologia , Prolapso de Órgão Pélvico/induzido quimicamente , Prolapso de Órgão Pélvico/epidemiologia , Estudos Retrospectivos , Estatísticas não Paramétricas , Ultrassonografia
17.
In Vivo ; 31(3): 335-341, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28438860

RESUMO

This study was undertaken to assess bone regeneration using hydroxyapatite (HA). The primary focus was comparison of bone regeneration between granular HA (gHA) forms and porous HA (pHA) scaffold. The extracted canine alveolar sockets were divided with three groups: control, gHA and pHA. Osteogenic effect in the gHA and pHA groups showed bone-specific surface and bone mineral density to be significantly higher than that of the control group (p<0.01). Bone volume fraction, bone mineral density, and amount of connective tissue related to disturbing osseointegration of the gHA group was higher than in the pHA group. Quantity of new bone formation of the pHA group was higher than that of the gHA group. This study demonstrated that gHA and pHA are potentially good bone substitutes for alveolar socket healing. For new bone formation during 8 weeks' post-implantation, HA with porous scaffold was superior to the granular form of HA.


Assuntos
Regeneração Óssea/efeitos dos fármacos , Regeneração Óssea/fisiologia , Substitutos Ósseos/farmacologia , Durapatita/farmacologia , Osteogênese/efeitos dos fármacos , Alvéolo Dental/efeitos dos fármacos , Animais , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/fisiologia , Cães , Masculino , Osseointegração/efeitos dos fármacos , Osseointegração/fisiologia , Osteogênese/fisiologia , Alvéolo Dental/fisiologia , Cicatrização/efeitos dos fármacos , Cicatrização/fisiologia
18.
Khirurgiia (Mosk) ; (3): 50-54, 2017.
Artigo em Russo | MEDLINE | ID: mdl-28374713

RESUMO

AIM: To study neocollagenogenesis after implantation of polypropylene endoprosthesis and polypropylene combined with polylactic acid endoprosthesis on background of «potassium orotate¼ administration. MATERIAL AND METHODS: We used two different types of endoprosthesis in the experiment. The first type was made of just polypropylene, the second type was made of polypropylene combined with polylactic acid. Histological examination was performed using polarizing microscopy. Collagen types I and III ratio in connective tissue around the prosthesis was analyzed according to the color that was individual for each type. RESULTS: The results were significantly better in case of collagenogenesis stimulation with Potassium orotate within 30 days and later for one type of endoprosthesis. Also we revealed that collagenogenesis and paraprosthesis capsule formation were more active in case of combined endoprosthesis. We revealed stimulating action of «Potassium Orotate¼ for collegenogenesis process, this fact was proved by increased collagen I/III ratio. CONCLUSION: Optimization of collagenogenesis was based on persistent 1,37-fold increase of collagen I/III ratio in case of combined endoprosthesis after 90 days. It was manifested by accelerated formation of connective tissue capsule and facilitated early isolation of the implant from surrounding tissues.


Assuntos
Colágeno/metabolismo , Tecido Conjuntivo , Implantes Experimentais , Ácido Orótico , Poliésteres/farmacologia , Polipropilenos/farmacologia , Implantação de Prótese/instrumentação , Regeneração/efeitos dos fármacos , Experimentação Animal , Animais , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/farmacologia , Disponibilidade Biológica , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/metabolismo , Tecido Conjuntivo/patologia , Camundongos , Ácido Orótico/metabolismo , Ácido Orótico/farmacologia , Compostos de Potássio/metabolismo , Compostos de Potássio/farmacologia , Implantação de Prótese/métodos , Regeneração/fisiologia
19.
Arch Dermatol Res ; 309(5): 389-396, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28324170

RESUMO

This study uses light and electron microscopes to gain a better knowledge of the interactions of calcium hydroxylapatite filler with the connective tissue of the skin and the modifications of the human deep dermis, after 2 months of treatment. Some morphological evidences of this observational study of filler treated tissue support-specific mechanism involved in the structural modifications of both filler microspherules and cells of the connective tissue. They demonstrate the absence of any immunological reaction and show that the used filler is modified very slowly over time by the action of cells of the connective tissue closely related to the filler without any activity of phagocytosis. Furthermore, associated with the modifications of the filler, evidences of stimulatory effects on dermal fibroblasts are reported.


Assuntos
Materiais Biocompatíveis/farmacologia , Tecido Conjuntivo/efeitos dos fármacos , Durapatita/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Pele/ultraestrutura , Feminino , Fibroblastos/fisiologia , Humanos , Microscopia Eletrônica
20.
Beijing Da Xue Xue Bao Yi Xue Ban ; 49(1): 169-75, 2017 02 18.
Artigo em Chinês | MEDLINE | ID: mdl-28203026

RESUMO

OBJECTIVE: To evaluate bone formation in human extraction sockets with absorbed surrounding walls augmented with Bio-Oss and Bio-Gide after a 6-month healing period by histologic and histomorphometric analyses. METHODS: Six fresh molar tooth extraction sockets in 6 patients who required periodontally compromised moral tooth extraction were included in this study. The six fresh extraction sockets were grafted with Bio-Oss particle covered with Bio-Gide. The 2.8 mm×6.0 mm cylindric bone specimens were taken from the graft sites with aid of stent 6 months after the surgery. Histologic and histomorphometric analyses were performed. RESULTS: The histological results showed Bio-Oss particles were easily distinguished from the newly formed bone, small amounts of new bone were formed among the Bio-Oss particles, large amounts of connective tissue were found. Intimate contact between the newly formed bone and the small part of Bio-Oss particles was present. All the biopsy cylinders measurement demonstrated a high inter-individual variability in the percentage of the bone, connective tissues and Bio-Oss particles. The new bone occupied 11.54% (0-28.40%) of the total area; the connective tissues were 53.42% (34.08%-74.59%) and the Bio-Oss particles were 35.04% (13.92%-50.87%). The percentage of the particles, which were in contact with bone tissues, amounted to 20.13% (0-48.50%). CONCLUSION: Sites grafted with Bio-Oss particles covered with Bio-Gide were comprised of connective tissues and small amounts of newly formed bone surrounding the graft particles.


Assuntos
Matriz Óssea/anatomia & histologia , Matriz Óssea/crescimento & desenvolvimento , Colágeno/farmacologia , Colágeno/uso terapêutico , Tecido Conjuntivo/anatomia & histologia , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/crescimento & desenvolvimento , Minerais/farmacologia , Minerais/uso terapêutico , Alvéolo Dental/anatomia & histologia , Alvéolo Dental/efeitos dos fármacos , Alvéolo Dental/crescimento & desenvolvimento , Matriz Óssea/efeitos dos fármacos , Substitutos Ósseos/uso terapêutico , Humanos , Dente Molar , Osteogênese/efeitos dos fármacos , Osteogênese/fisiologia , Extração Dentária , Alvéolo Dental/lesões , Cicatrização/efeitos dos fármacos , Cicatrização/fisiologia
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