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Adv Exp Med Biol ; 1232: 375-381, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31893434


The value of optical redox imaging (ORI) of cells/tissues based on the intrinsic fluorescences of NADH (nicotinamide adenine dinucleotide) and oxidized flavoproteins (containing flavin adenine dinucleotide, i.e., FAD) has been demonstrated for potential biomedical applications including diagnosis, prognosis, and determining treatment response. However, the Chance redox scanner (a 3D cryogenic tissue imager) is limited by spatial resolution (~50 µm), and tissue ORI using fluorescence microscopy (single or multi-photon) is limited by the light penetration depth. Furthermore, viable or snap-frozen tissues are usually required. In this project, we aimed to study whether ORI may be achieved for unstained fixed tissue using a state-of-the-art modern Serial Two-Photon (STP) Tomography scanner that can rapidly acquire multi-plane images at micron resolution. Tissue specimens of mouse muscle, liver, and tumor xenografts were harvested and fixed in 4% paraformaldehyde (PFA) for 24 h. Tissue blocks were scanned by STP Tomography under room temperature to acquire the autofluorescence signals (NADH channel: excitation 750 nm, blue emission filter; FAD channel: excitation 860 nm, green emission filter). We observed remarkable signals with significant intra-tissue heterogeneity in images of NADH, FAD and redox ratio (FAD/(NADH+FAD)), which are worthy of further investigation for extracting biological information.

Tecnologia Biomédica , NAD , Imagem Óptica , Animais , Tecnologia Biomédica/instrumentação , Tecnologia Biomédica/métodos , Estudos de Viabilidade , Flavina-Adenina Dinucleotídeo , Xenoenxertos/diagnóstico por imagem , Camundongos , Oxirredução , Fótons
Ann R Coll Surg Engl ; 102(1): 3-8, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31858833


INTRODUCTION: Achieving a standard of clinical research at the pinnacle of the evidence pyramid is historically expensive and logistically challenging. Research collaboratives have delivered high-impact prospective multicentre audits and clinical trials by using trainee networks with a range of enabling technology. This review outlines such use of technology in the UK and provides a framework of recommended technologies for future studies. METHODS: A review of the literature identified technology used in collaborative projects. Additional technologies were identified through web searches. Technologies were grouped into themes including access (networking and engagement), collaboration and event organisation. The technologies available to support each theme were studied further to outline relative benefits and limitations. FINDINGS: Thirty-three articles from trainee research collaboratives were identified. The most frequently documented technologies were social media applications, website platforms and research databases. The Supportive Technologies in Collaborative Research framework is proposed, providing a structure for using the technologies available to support multicentre collaboration. Such technologies are often overlooked in the literature by established and start-up collaborative project groups. If used correctly, they might help to overcome the physical, logistical and financial barriers of multicentre clinical trials.

Pesquisa Biomédica/métodos , Tecnologia Biomédica/métodos , Comportamento Cooperativo , Relações Interprofissionais , Ensaios Clínicos como Assunto , Comunicação , Cirurgia Geral/educação , Humanos , Internet , Redes Sociais Online , Estudantes de Medicina
RECIIS (Online) ; 13(4): 911-921, out.-dez. 2019. ilus
Artigo em Português | LILACS | ID: biblio-1047676


Uma vez que a internet assumiu o papel de fonte preferencial e espontânea de acesso à informação, o objetivo deste estudo é sintetizar o que as mães brasileiras buscam e encontram na internet sobre aleitamento materno, por meio de revisão crítica da literatura científica dos últimos 10 anos. Os estudos selecionados permitiram a categorização de três tópicos de discussão: qualidade das informações disponíveis; desenvolvimento de facilitadores para acesso a informações sobre aleitamento materno; e grupos virtuais de apoio à amamentação como espaço de educação em saúde. A internet é uma área com grande potencial para o desenvolvimento de ações de educação em saúde e promoção do aleitamento materno. Apesar de pertinente e atual, a literatura científica carece de pesquisas que explorem o tema do aleitamento materno sob o ponto de vista da interação on-line das mães, sendo este um campo muito rico para estudos futuros.

Since the internet has assumed the role of a preferential and spontaneous source of access to information, this work intends to synthesize what Brazilian mothers search and find on the internet about breastfeeding through a Critical Review of Literature in the last 10 years. The selected studies have allowed for the categorization of three discussion topics: quality of information available, development of facilitators for access to information on breastfeeding and virtual groups supporting breastfeeding as a space for health education. The internet is an area with great potential for the development of actions of health education and promotion of breastfeeding. Although pertinent and current, the scientific literature lacks research that explores the topic of breastfeeding from the perspective of the online interaction of mothers, which is a very rich field for future studies.

El objetivo de este estudio es sintetizar lo que las madres brasileñas buscan y encuentran en internet sobre lactancia materna, por medio de una revisión crítica de la literatura científica de los últimos 10 años. Los estudios seleccionados permitieron la categorización de tres temas de discusión: calidad de la información disponible; desarrollo de facilitadores para acceso a informaciones sobre lactancia materna y grupos virtuales de apoyo a la lactancia como espacio de educación en salud. La internet es un área con gran potencial para el desarrollo de acciones de educación en salud y promoción de la lactancia materna. A pesar de pertinente y actual, la literatura científica carece de investigaciones que exploren el tema de la lactancia materna desde el punto de vista de la interacción online de las madres, siendo este un campo muy rico para estudios futuros.

Humanos , Aleitamento Materno , Saúde Materno-Infantil , Internet , Tecnologia Biomédica , Promoção da Saúde , Socialização , Mulheres , Revisão , Poder Familiar , Nutrição do Lactente , Mídias Sociais , Mães
Adv Exp Med Biol ; 1174: 223-263, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31713201


Nanofibrillar forms of amyloidogenic proteins were initially discovered in the context of protein misfolding and disease but have more recently been found at the origin of key biological functionality in many naturally occurring functional materials, such as adhesives and biofilm coatings. Their physiological roles in nature reflect their great strength and stability, which has led to the exploration of their use as the basis of artificial protein-based functional materials. Particularly for biomedical applications, they represent attractive building blocks for the development of, for instance, drug carrier agents due to their inherent biocompatibility and biodegradability. Furthermore, the propensity of proteins to self-assemble into amyloid fibrils can be exploited under microconfinement, afforded by droplet microfluidic techniques. This approach allows the generation of multi-scale functional microgels that can host biological additives and can be designed to incorporate additional functionality, such as to aid targeted drug delivery.

Proteínas Amiloidogênicas , Géis , Proteínas Amiloidogênicas/química , Proteínas Amiloidogênicas/metabolismo , Tecnologia Biomédica/tendências , Géis/química , Microfluídica
Adv Exp Med Biol ; 1174: 291-312, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31713203


Fe3O4, also called magnetite, is a naturally occurring mineral and has been widely used in biomedical applications. However, in the past, all the applications were based on its excellent magnetic properties and neglected its catalytic properties. In 2007, we found that Fe3O4 nanoparticles are able to perform intrinsic enzyme-like activities. A specific term, "nanozyme", is used to describe the new property of intrinsic enzymatic activity of nanomaterials. Since then, Fe3O4 nanoparticles have been used as enzyme mimics, which broadens their applications beyond simply their magnetic properties, with applications in biomedical diagnosis and therapy, environmental monitoring and treatment, the food industry and chemical synthesis. In this chapter, we will summarize the basic features of Fe3O4 as an enzyme mimetic and its applications in biomedicine.

Biomimética , Nanopartículas de Magnetita , Tecnologia Biomédica/tendências , Catálise , Enzimas/metabolismo , Nanopartículas de Magnetita/química
Biomed Instrum Technol ; 53(6): 409, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31765590
Biomed Instrum Technol ; 53(6): 438-442, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31765591
Int J Nanomedicine ; 14: 7643-7663, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31571869


Angiogenesis is the formation of new blood vessels from pre-existing vessels. It is a highly regulated process as determined by the interplay between pro-angiogenic and anti-angiogenic factors. Under certain conditions the balance between angiogenesis stimulators and inhibitors is altered, which results in a shift from physiological to pathological angiogenesis. Therefore, the goal of therapeutic targeting of angiogenic process is to normalize vasculature in target tissues by enhancing angiogenesis in disease conditions of reduced vascularity and blood flow, such as tissue ischemia, or alternatively to inhibit excessive and abnormal angiogenesis in disorders like cancer. Gold nanoparticles (AuNPs) are special particles that are generated by nanotechnology and composed of an inorganic core containing gold which is encircled by an organic monolayer. The ability of AuNPs to alter vasculature has captured recent attention in medical literature as potential therapeutic agents for the management of pathologic angiogenesis. This review provides an overview of the effects of AuNPs on angiogenesis and the molecular mechanisms and biomedical applications associated with their effects. In addition, the main synthesis methods, physical properties, uptake mechanisms, and toxicity of AuNPs are briefly summarized.

Tecnologia Biomédica/métodos , Ouro/uso terapêutico , Nanopartículas Metálicas/uso terapêutico , Neovascularização Patológica/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Endocitose , Ouro/toxicidade , Humanos , Nanopartículas Metálicas/toxicidade
Rev Infirm ; 68(254): 25-26, 2019 Oct.
Artigo em Francês | MEDLINE | ID: mdl-31587846


The aim of the East Hérault (34) and Sud Aveyron (12) hospital group was to pave the way for patients and organise their management. A reflection process demonstrated interest in digital technology. Its common use will ensure maintenance of the relationship between all actors involved with the same patient.

Tecnologia Biomédica , Relações Interprofissionais , Corpo Clínico Hospitalar/psicologia , França , Hospitais , Humanos
Pflege ; 32(6): 324-333, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31576771


The potential of technological assistance to support distance caregiving - literature review and empirical results Abstract. Background: Due to demographic change, increasing labour mobility and changing family patterns, social relationships often exist over long distances. Supporting relatives over a distance is therefore a highly topical issue but still little discussed, also in Germany and Switzerland. Aim: The project "DiCa" (2016 - 2019) with an interdisciplinary research team from Germany (EH Ludwigsburg) and Switzerland (Careum School of Health, Zurich) aims to investigate different dimensions of "Distance Caregiving". This paper deals with the possible use of new technologies to support these care arrangements. Methods: Based on a literature review, qualitative interviews were conducted with "Distance Carers" and partner companies in Germany to investigate the use of new technologies in the context of "Distance Caregiving". Results: There are initial approaches concerning technical solutions in home care and in companies. So far communication options via telephone and smartphone and flexible working time and workplace regulations have played an important role. However, the potential of new technologies does not seem to be fully explored. Conclusions: In order to make the most of the various possibilities of innovative technologies in the context of "Distance Caregiving" for those affected but also for companies, well-researched information and independent advice and counseling are required for all parties involved in the care process.

Tecnologia Biomédica , Cuidadores , Invenções , Telemedicina/instrumentação , Humanos
Pflege ; 32(6): 334-342, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31640473


Technology commitment in outpatient care Abstract. Background: New technologies are becoming increasingly important in outpatient care. The willingness of professional carers to use new technologies is often considered to be low, especially where older and female carers are concerned who play a large role in outpatient care. However, reliable data on technology commitment in outpatient care are not yet available for German-speaking countries. AIM: This paper aims to provide insights into the state of technology commitment in outpatient care. METHODS: For data collection, the standardised assessment of technology commitment was used, which determines technology readiness via the facets "technology acceptance", "technology competence conviction" and "technology control conviction" (26-2Neyer et al., 2012). A first data collection (2013) concentrated on nursing services in the federal state of Lower Saxony (n = 263), a second data collection (2017) was carried out with a nationwide care service provider (n = 593). RESULTS: For the first time, the results of the present investigations provide differentiated insights into questions of technology commitment in outpatient care in Germany. In particular, there are indications of differences in the willingness to use technology in outpatient care depending on the age group of the interviewees. CONCLUSION: The introduction of new technologies into the everyday life of caregivers requires demographically sensitive concepts for preparing and supporting the users.

Assistência Ambulatorial , Atitude do Pessoal de Saúde , Tecnologia Biomédica , Cuidadores/psicologia , Alemanha , Humanos
Rev. Pesqui. (Univ. Fed. Estado Rio J., Online) ; 11(5): 1219-1225, out.-dez. 2019. tab
Artigo em Inglês, Português | LILACS, BDENF - Enfermagem | ID: biblio-1022336


Objetivo: Avaliar os efeitos da irradiação ultrassônica de baixa frequência no tratamento de úlcera venosa. Método: Estudo de aplicação de tecnologia em saúde, realizado com indivíduos com lesões venosas, no ano de 2017 entre os meses de abril a agosto, utilizado formulário estruturado para coleta, analisados no programa Excel e representados em tabela e estatística descritiva. Resultados: Participaram cinco pacientes, três mulheres entre 65 e 88 anos de idade. Os participantes apresentaram cinco úlceras venosas, maioria localizada na porção inferior da perna. Houve redução mínima de 2,5% e máxima de 35,8% sobre a área das lesões. Um participante apresentou 100,0% de epitelização e os demais apresentaram tecido de granulação maior ou igual a 70,0%. Encontrou-se redução máxima de 10 pontos na PUSH, redução mínima de 2 pontos. Conclusão: A terapia ultrassônica traz aspectos positivos para o processo de reparação tecidual

Objective: The study's purpose has been to assess the effects of low-frequency ultrasound irradiation in the treatment of venous ulcer. Methods: It is a health technology application study, which was performed with individuals bearing venous lesions. The research was carried out over 2017 from April to August, using a structured form for data collection, where such data was analyzed in the Excel program and represented through tables and descriptive statistics. Results: Five patients have participated, being three women within the age group from 65 to 88 years old. The participants had five venous ulcers, predominantly located in the leg's lower portion. There was a reduction in the lesion area ranging from 2.5% (minimum) to 35.8% (maximum). One participant achieved 100% epithelization, and the others showed granulation tissue greater or equal to 70.0%. There was a maximum reduction of 10 points in the Pressure Ulcer Scale for Healing (PUSH), and a minimum reduction of 2 points. Conclusion: The low-frequency ultrasound therapy produces positive aspects to the tissue healing process

Objetivo: Evaluar los efectos de la irradiación ultrasónica de baja frecuencia en el tratamiento de úlcera venosa. Método: Estudio de aplicación de tecnología en salud, realizado con individuos con lesiones venosas, en el año 2017 entre los meses abril a agosto, utilizado formulario estructurado para recolección, analizados en el programa Excel y representados en tabla y estadística descriptiva. Resultados: Participaron cinco pacientes, tres mujeres entre 65 y 88 años de edad. Presentaron cinco úlceras venosas, mayoría localizada en la porción inferior de la pierna. Se observó una reducción mínima del 2,5% y una máxima del 35,8% sobre el área de las lesiones. Un participante presentó 100,0% de epitelización y los demás presentaron tejido de granulación mayor o igual al 70,0%. Se encontró reducción máxima de 10 puntos en la PUSH, reducción mínima de 2 puntos. Conclusión: La terapia ultrasónica trae aspectos positivos para el proceso de reparación del tejido

Humanos , Masculino , Feminino , Idoso , Idoso de 80 Anos ou mais , Terapia por Ultrassom/enfermagem , Úlcera Varicosa/terapia , Cicatrização , Ferimentos e Lesões/enfermagem , Ferimentos e Lesões/terapia , Tecnologia Biomédica/métodos