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2.
Adv Exp Med Biol ; 1232: 375-381, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31893434

RESUMO

The value of optical redox imaging (ORI) of cells/tissues based on the intrinsic fluorescences of NADH (nicotinamide adenine dinucleotide) and oxidized flavoproteins (containing flavin adenine dinucleotide, i.e., FAD) has been demonstrated for potential biomedical applications including diagnosis, prognosis, and determining treatment response. However, the Chance redox scanner (a 3D cryogenic tissue imager) is limited by spatial resolution (~50 µm), and tissue ORI using fluorescence microscopy (single or multi-photon) is limited by the light penetration depth. Furthermore, viable or snap-frozen tissues are usually required. In this project, we aimed to study whether ORI may be achieved for unstained fixed tissue using a state-of-the-art modern Serial Two-Photon (STP) Tomography scanner that can rapidly acquire multi-plane images at micron resolution. Tissue specimens of mouse muscle, liver, and tumor xenografts were harvested and fixed in 4% paraformaldehyde (PFA) for 24 h. Tissue blocks were scanned by STP Tomography under room temperature to acquire the autofluorescence signals (NADH channel: excitation 750 nm, blue emission filter; FAD channel: excitation 860 nm, green emission filter). We observed remarkable signals with significant intra-tissue heterogeneity in images of NADH, FAD and redox ratio (FAD/(NADH+FAD)), which are worthy of further investigation for extracting biological information.


Assuntos
Tecnologia Biomédica , NAD , Imagem Óptica , Animais , Tecnologia Biomédica/instrumentação , Tecnologia Biomédica/métodos , Estudos de Viabilidade , Flavina-Adenina Dinucleotídeo , Xenoenxertos/diagnóstico por imagem , Camundongos , Oxirredução , Fótons
3.
Ann R Coll Surg Engl ; 102(1): 3-8, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31858833

RESUMO

INTRODUCTION: Achieving a standard of clinical research at the pinnacle of the evidence pyramid is historically expensive and logistically challenging. Research collaboratives have delivered high-impact prospective multicentre audits and clinical trials by using trainee networks with a range of enabling technology. This review outlines such use of technology in the UK and provides a framework of recommended technologies for future studies. METHODS: A review of the literature identified technology used in collaborative projects. Additional technologies were identified through web searches. Technologies were grouped into themes including access (networking and engagement), collaboration and event organisation. The technologies available to support each theme were studied further to outline relative benefits and limitations. FINDINGS: Thirty-three articles from trainee research collaboratives were identified. The most frequently documented technologies were social media applications, website platforms and research databases. The Supportive Technologies in Collaborative Research framework is proposed, providing a structure for using the technologies available to support multicentre collaboration. Such technologies are often overlooked in the literature by established and start-up collaborative project groups. If used correctly, they might help to overcome the physical, logistical and financial barriers of multicentre clinical trials.


Assuntos
Pesquisa Biomédica/métodos , Tecnologia Biomédica/métodos , Comportamento Cooperativo , Relações Interprofissionais , Ensaios Clínicos como Assunto , Comunicação , Cirurgia Geral/educação , Humanos , Internet , Redes Sociais Online , Estudantes de Medicina
5.
Int J Nanomedicine ; 14: 7643-7663, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31571869

RESUMO

Angiogenesis is the formation of new blood vessels from pre-existing vessels. It is a highly regulated process as determined by the interplay between pro-angiogenic and anti-angiogenic factors. Under certain conditions the balance between angiogenesis stimulators and inhibitors is altered, which results in a shift from physiological to pathological angiogenesis. Therefore, the goal of therapeutic targeting of angiogenic process is to normalize vasculature in target tissues by enhancing angiogenesis in disease conditions of reduced vascularity and blood flow, such as tissue ischemia, or alternatively to inhibit excessive and abnormal angiogenesis in disorders like cancer. Gold nanoparticles (AuNPs) are special particles that are generated by nanotechnology and composed of an inorganic core containing gold which is encircled by an organic monolayer. The ability of AuNPs to alter vasculature has captured recent attention in medical literature as potential therapeutic agents for the management of pathologic angiogenesis. This review provides an overview of the effects of AuNPs on angiogenesis and the molecular mechanisms and biomedical applications associated with their effects. In addition, the main synthesis methods, physical properties, uptake mechanisms, and toxicity of AuNPs are briefly summarized.


Assuntos
Tecnologia Biomédica/métodos , Ouro/uso terapêutico , Nanopartículas Metálicas/uso terapêutico , Neovascularização Patológica/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Endocitose , Ouro/toxicidade , Humanos , Nanopartículas Metálicas/toxicidade
6.
Rev. Pesqui. (Univ. Fed. Estado Rio J., Online) ; 11(5): 1219-1225, out.-dez. 2019. tab
Artigo em Inglês, Português | LILACS, BDENF - Enfermagem | ID: biblio-1022336

RESUMO

Objetivo: Avaliar os efeitos da irradiação ultrassônica de baixa frequência no tratamento de úlcera venosa. Método: Estudo de aplicação de tecnologia em saúde, realizado com indivíduos com lesões venosas, no ano de 2017 entre os meses de abril a agosto, utilizado formulário estruturado para coleta, analisados no programa Excel e representados em tabela e estatística descritiva. Resultados: Participaram cinco pacientes, três mulheres entre 65 e 88 anos de idade. Os participantes apresentaram cinco úlceras venosas, maioria localizada na porção inferior da perna. Houve redução mínima de 2,5% e máxima de 35,8% sobre a área das lesões. Um participante apresentou 100,0% de epitelização e os demais apresentaram tecido de granulação maior ou igual a 70,0%. Encontrou-se redução máxima de 10 pontos na PUSH, redução mínima de 2 pontos. Conclusão: A terapia ultrassônica traz aspectos positivos para o processo de reparação tecidual


Objective: The study's purpose has been to assess the effects of low-frequency ultrasound irradiation in the treatment of venous ulcer. Methods: It is a health technology application study, which was performed with individuals bearing venous lesions. The research was carried out over 2017 from April to August, using a structured form for data collection, where such data was analyzed in the Excel program and represented through tables and descriptive statistics. Results: Five patients have participated, being three women within the age group from 65 to 88 years old. The participants had five venous ulcers, predominantly located in the leg's lower portion. There was a reduction in the lesion area ranging from 2.5% (minimum) to 35.8% (maximum). One participant achieved 100% epithelization, and the others showed granulation tissue greater or equal to 70.0%. There was a maximum reduction of 10 points in the Pressure Ulcer Scale for Healing (PUSH), and a minimum reduction of 2 points. Conclusion: The low-frequency ultrasound therapy produces positive aspects to the tissue healing process


Objetivo: Evaluar los efectos de la irradiación ultrasónica de baja frecuencia en el tratamiento de úlcera venosa. Método: Estudio de aplicación de tecnología en salud, realizado con individuos con lesiones venosas, en el año 2017 entre los meses abril a agosto, utilizado formulario estructurado para recolección, analizados en el programa Excel y representados en tabla y estadística descriptiva. Resultados: Participaron cinco pacientes, tres mujeres entre 65 y 88 años de edad. Presentaron cinco úlceras venosas, mayoría localizada en la porción inferior de la pierna. Se observó una reducción mínima del 2,5% y una máxima del 35,8% sobre el área de las lesiones. Un participante presentó 100,0% de epitelización y los demás presentaron tejido de granulación mayor o igual al 70,0%. Se encontró reducción máxima de 10 puntos en la PUSH, reducción mínima de 2 puntos. Conclusión: La terapia ultrasónica trae aspectos positivos para el proceso de reparación del tejido


Assuntos
Humanos , Masculino , Feminino , Idoso , Idoso de 80 Anos ou mais , Terapia por Ultrassom/enfermagem , Úlcera Varicosa/terapia , Cicatrização , Ferimentos e Lesões/enfermagem , Ferimentos e Lesões/terapia , Tecnologia Biomédica/métodos
7.
J Drugs Dermatol ; 18(9): 904-908, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31524346

RESUMO

Dermatologists are among the most inventive physicians, trained in the multiple disciplines of medical dermatology, surgical dermatology, and dermatopathology. Many of the advances in dermatology practice have been derived from inventive colleagues who identify opportunities for improvement in practice, develop viable prototypes to address these practice opportunities, and persevere through the hard work of developing new technologies to advance the practice of dermatology. In this article, we will review the basic elements of invention, patents, and the range of outcomes associated with the pursuit of invention. Examples of innovative dermatologic technologies and approaches will be reviewed. Opportunities abound for dermatologists to contribute to the advancement of medical care through invention in our specialty. J Drugs Dermatol. 2019;18(9):904-908.


Assuntos
Tecnologia Biomédica/métodos , Dermatologia/métodos , Invenções/legislação & jurisprudência , Dermatopatias/terapia , Tecnologia Biomédica/legislação & jurisprudência , Fármacos Dermatológicos/uso terapêutico , Dermatologistas , Dermatologia/instrumentação , Dermatologia/legislação & jurisprudência , Desenho de Equipamento , Humanos , Patentes como Assunto , Dermatopatias/diagnóstico
8.
Int J Nanomedicine ; 14: 4781-4800, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31308658

RESUMO

Advancements in nanotechnology and molecular biology have promoted the development of a diverse range of models to intervene in various disorders (from diagnosis to treatment and even theranostics). Manganese dioxide nanosheets (MnO2 NSs), a typical two-dimensional (2D) transition metal oxide of nanomaterial that possesses unique structure and distinct properties have been employed in multiple disciplines in recent decades, especially in the field of biomedicine, including biocatalysis, fluorescence sensing, magnetic resonance imaging and cargo-loading functionality. A brief overview of the different synthetic methodologies for MnO2 NSs and their state-of-the-art biomedical applications is presented below, as well as the challenges and future perspectives of MnO2 NSs.


Assuntos
Tecnologia Biomédica/métodos , Compostos de Manganês/química , Nanoestruturas/química , Nanotecnologia/métodos , Óxidos/química , Técnicas Biossensoriais , Nanoestruturas/toxicidade , Óxidos/toxicidade , Tamanho da Partícula
9.
Sensors (Basel) ; 19(9)2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31075985

RESUMO

Over the past few decades, we have witnessed a dramatic rise in life expectancy owing to significant advances in medical science and technology, medicine as well as increased awareness about nutrition, education, and environmental and personal hygiene. Consequently, the elderly population in many countries are expected to rise rapidly in the coming years. A rapidly rising elderly demographics is expected to adversely affect the socioeconomic systems of many nations in terms of costs associated with their healthcare and wellbeing. In addition, diseases related to the cardiovascular system, eye, respiratory system, skin and mental health are widespread globally. However, most of these diseases can be avoided and/or properly managed through continuous monitoring. In order to enable continuous health monitoring as well as to serve growing healthcare needs; affordable, non-invasive and easy-to-use healthcare solutions are critical. The ever-increasing penetration of smartphones, coupled with embedded sensors and modern communication technologies, make it an attractive technology for enabling continuous and remote monitoring of an individual's health and wellbeing with negligible additional costs. In this paper, we present a comprehensive review of the state-of-the-art research and developments in smartphone-sensor based healthcare technologies. A discussion on regulatory policies for medical devices and their implications in smartphone-based healthcare systems is presented. Finally, some future research perspectives and concerns regarding smartphone-based healthcare systems are described.


Assuntos
Smartphone , Tecnologia Biomédica/métodos , Assistência à Saúde/métodos , Humanos , Monitorização Ambulatorial , Telemedicina/métodos
10.
Int J Nanomedicine ; 14: 2349-2369, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31040661

RESUMO

Background: In view of the growing importance of nanotechnologies, the detection/identification of nanoparticles type has been considered of utmost importance. Although the characterization of synthetic/organic nanoparticles is currently considered a priority (eg, drug delivery devices, nanotextiles, theranostic nanoparticles), there are many examples of "naturally" generated nanostructures - for example, extracellular vesicles (EVs), lipoproteins, and virus - that provide useful information about human physiology or clinical conditions. For example, the detection of tumor-related exosomes, a specific type of EVs, in circulating fluids has been contributing to the diagnosis of cancer in an early stage. However, scientists have struggled to find a simple, fast, and low-cost method to accurately detect/identify these nanoparticles, since the majority of them have diameters between 100 and 150 nm, thus being far below the diffraction limit. Methods: This study investigated if, by projecting the information provided from short-term portions of the back-scattered laser light signal collected by a polymeric lensed optical fiber tip dipped into a solution of synthetic nanoparticles into a lower features dimensional space, a discriminant function is able to correctly detect the presence of 100 nm synthetic nanoparticles in distilled water, in different concentration values. Results and discussion: This technique ensured an optimal performance (100% accuracy) in detecting nanoparticles for a concentration above or equal to 3.89 µg/mL (8.74E+10 particles/mL), and a performance of 90% for concentrations below this value and higher than 1.22E-03 µg/mL (2.74E+07 particles/mL), values that are compatible with human plasmatic levels of tumor-derived and other types of EVs, as well as lipoproteins currently used as potential biomarkers of cardiovascular diseases. Conclusion: The proposed technique is able to detect synthetic nanoparticles whose dimensions are similar to EVs and other "clinically" relevant nanostructures, and in concentrations equivalent to the majority of cell-derived, platelet-derived EVs and lipoproteins physiological levels. This study can, therefore, provide valuable insights towards the future development of a device for EVs and other biological nanoparticles detection with innovative characteristics.


Assuntos
Tecnologia Biomédica/métodos , Técnicas Biossensoriais/métodos , Nanopartículas/química , Fibras Ópticas , Análise Discriminante , Exossomos/química , Vesículas Extracelulares , Análise de Fourier , Humanos , Polímeros/química , Soluções
11.
Maturitas ; 125: 17-19, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31133211

RESUMO

Our objective was to bridge the gap between gerontechnology developers and hospitalized frail elderly people, in order to promote open gerontechnology innovation in hospitals. We designed a hospital-based living lab that provides reflexive "idea incubator workshops" that gather both the users and the developers of technology, supplemented with an "experimental hospital room" for the testing of devices by older inpatients. The ALLEGRO living lab was delivered in 2018 at the Geriatric Department of Angers University Hospital, France. The workshops and experimental hospital room should help frail older inpatients to participate in the co-design and co-development of new technologies to improve hospital care and promote successful aging.


Assuntos
Atividades Cotidianas , Envelhecimento , Tecnologia Biomédica/métodos , Idoso Fragilizado , Geriatria/métodos , Idoso , Idoso de 80 Anos ou mais , Tecnologia Biomédica/instrumentação , Cuidadores , Difusão de Inovações , Arquitetura de Instituições de Saúde , França , Avaliação Geriátrica , Geriatria/instrumentação , Metas , Serviços de Saúde para Idosos , Hospitais , Humanos , Pacientes Internados , Tecnologia
12.
Mater Sci Eng C Mater Biol Appl ; 100: 535-543, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30948090

RESUMO

In this study, zinc-sulfate-calcium-phosphate (ZSCAP) ceramics was prepared by calcination in the presence of microwave irradiation using precursors ZnSO4:ZnO:CaO:P2O5 in a ratio of 15:30:30:25 (by weight). The calcined ZSCAP ceramics was mixed with microcrystalline cellulose and it was further heated by microwave radiation for the preparation of cellulose/ZSCAP nanocomposites. It was found that microwave heating time played an important role in the crystalline phase of synthesized nanocomposites of cellulose/ZSCAP. Well-crystalline phases of calcium phosphate, zinc oxide and zinc sulfate were observed in the nanocomposites at 20 min of microwave heating time. Fourier transformed infrared spectroscopy (FTIR) and Raman spectroscopy confirmed that the obtained products were of cellulose/ZSCAP nanocomposites. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) images indicated that reinforced ZSCAP nanostructures were embedded into cellulose matrix. Energy dispersive X-ray spectroscopy (EDS) further supported the presence of Zn, S, Ca and P in cellulose/ZSCAP nanocomposites. The thermal behavior of the products was studied using thermogravimetric analysis (TGA) and differential thermal analysis (DTA). The prepared nanocomposites showed antibacterial activity against Staphylococcus aureus and Escherichia coli. In vitro cytotoxicity assay revealed that the prepared nanocomposites had no influence on proliferation of MG-63 cells. This rapid microwave-assisted method is simple, fast and suitable for the production of cellulose/ZSCAP nanocomposites, which finds its biomedical applications in tissue engineering and bone repair.


Assuntos
Tecnologia Biomédica/métodos , Fosfatos de Cálcio/síntese química , Celulose/síntese química , Micro-Ondas , Nanocompostos/química , Sulfato de Zinco/síntese química , Antibacterianos/farmacologia , Fosfatos de Cálcio/química , Fosfatos de Cálcio/farmacologia , Varredura Diferencial de Calorimetria , Linhagem Celular Tumoral , Celulose/química , Celulose/farmacologia , Análise Diferencial Térmica , Escherichia coli/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Nanocompostos/ultraestrutura , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral Raman , Staphylococcus aureus/efeitos dos fármacos , Termogravimetria , Difração de Raios X , Sulfato de Zinco/química , Sulfato de Zinco/farmacologia
13.
Mater Sci Eng C Mater Biol Appl ; 100: 608-615, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30948097

RESUMO

A series of Zn-doped hybrid materials based on silica from tetraethoxysilane (TEOS) and hydroxypropyl cellulose (HPC) were prepared by a sol-gel route. The structure, morphology and thermal behavior of synthesized hybrids were characterized by infrared (IR) spectroscopy, ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM) and differential thermal analysis with thermogravimetric analysis (DTA/TG). The obtained materials were investigated for a potential biomedical application. The antibacterial properties of hybrids were investigated by measuring the inhibition zones formed around the materials containing different zinc content in presence of reference strains of Gram-positive and Gram-negative bacteria. The biocompatibility tests showed no cytotoxicity and genotoxicity, as well as no changes in actin cytoskeleton organization for hybrids with Zn content below 5 wt%.


Assuntos
Tecnologia Biomédica/métodos , Celulose/análogos & derivados , Nanopartículas/química , Dióxido de Silício/química , Zinco/química , Citoesqueleto de Actina/efeitos dos fármacos , Citoesqueleto de Actina/metabolismo , Animais , Antibacterianos/farmacologia , Bacillus subtilis/efeitos dos fármacos , Bacillus subtilis/crescimento & desenvolvimento , Morte Celular/efeitos dos fármacos , Divisão Celular , Linhagem Celular , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Celulose/química , Análise Diferencial Térmica , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Fibroblastos/citologia , Camundongos , Testes de Sensibilidade Microbiana , Espectrofotometria Infravermelho , Espectrofotometria Ultravioleta
14.
Mater Sci Eng C Mater Biol Appl ; 100: 616-630, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30948098

RESUMO

With advances in nanotechnology, the applications of nanomaterial are developing widely and greatly. The characteristic properties of carbon nanotubes (CNTs) make them the most selective candidate for various multi-functional applications. The greater surface area of the CNTs in addition to the capability to manipulate the surfaces and dimensions has provided greater potential for this nanomaterial. The CNTs possess greater potential for applications in biomedicine due to their vital electrical, chemical, thermal, and mechanical properties. The unique properties of CNT are exploited for numerous applications in the biomedical field. They are useful in both therapeutic and diagnostic applications. They form novel carrier systems which are also capable of site-specific delivery of therapeutic agents. In addition, CNTs are of potential application in biosensing. Many recently reported advanced systems of CNT could be exploited for their immense potential in biomedicine in the future.


Assuntos
Tecnologia Biomédica/métodos , Nanotubos de Carbono/química , Sistemas de Liberação de Medicamentos , Técnicas de Transferência de Genes , Nanotubos de Carbono/toxicidade , Nanotubos de Carbono/ultraestrutura , Medicina Regenerativa , Engenharia Tecidual
15.
Mater Sci Eng C Mater Biol Appl ; 101: 76-87, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31029366

RESUMO

The new green-synthesised ZnO nanoparticles (NPs) using apple (var. Starking) phytochemicals present a great potential for bioimaging applications. These NPs, when compared with ZnO microparticles synthesised with pure phytochemicals (quercetin or sucrose), and water, revealed that sizes and shapes were widely dependent on the organic precursors used. Based on these findings, new insights into the synthesis of ZnO NPs using apple phytochemicals were presented. The photoluminescent properties, characterized by steady-state and time resolve photoluminescence measurements, revealed that besides the intense sharp near-UV band edge emission observed for all particles, with sub-nanosecond lifetime, a strong broad emission band peak at 2.20 eV was detected for microparticles, with longer decay times being associate to crystal defects. Additionally, the photoluminescent properties of ZnO particles, further explored by fluorescence lifetime imaging microscopy (FLIM), suggested adequacy for imaging applications. The cytotoxicity, evaluated in human dermal fibroblasts, proving the biosafety of ZnO NPs by an unaffected cellular viability, total mitochondrial activity and F-actin cytoskeleton organization, contrasted with some degree of cytotoxicity depicted for microparticles. The influence of the phytochemicals in ZnO cytotoxicity was discussed. To the authors' best knowledge this is the first report of ZnO NPs synthesised with apple extracts. The novelty of choosing a fruit widely used in the food industry will render affordable NPs through the concept of circular economy. The proved biosafety of these ZnO NPs together with their intrinsic photoluminescent properties, open perspectives for the development of cost-effective bioimaging materials with potential to be further directed into biomedical applications.


Assuntos
Materiais Biocompatíveis/farmacologia , Tecnologia Biomédica/métodos , Luminescência , Malus/química , Nanopartículas Metálicas/química , Compostos Fitoquímicos/farmacologia , Óxido de Zinco/química , Morte Celular/efeitos dos fármacos , Derme/citologia , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Fluorescência , Humanos , Nanopartículas Metálicas/ultraestrutura , Compostos Fitoquímicos/química , Soluções , Espectrofotometria Ultravioleta
16.
Mater Sci Eng C Mater Biol Appl ; 101: 111-119, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31029304

RESUMO

The aim of this study was to tailor the deposition parameters of magnetron sputtering to synthetize tantalum oxide (TaxOy) films onto commercially pure titanium (cpTi) surface. The structural and optical properties, morphology, roughness, elemental chemical composition and surface energy were assessed. The impact of TaxOy films on initial Streptococcus sanguinis adhesion was investigated. The morphology and spreading of pre-osteoblastic (MC3T3-E1) cells on a crystalline tantalum oxide film were evaluated. TaxOy films with estimated thickness of 600 nm and different structures (amorphous or crystalline) were produced depending on the various oxygen flow rates and parameters used. X-ray diffraction analysis revealed that the 8 O2 sccm (600 °C/400 W) group showed crystallization corresponding to the ß-Ta2O5 phase. Optical analysis showed that the 4 O2 sccm (200 °C 300 W) to 8 O2 sccm (600 °C 300 W) groups and 10 O2 sccm (200 °C 300 W) group presented regular and large-amplitude interference oscillations, suggesting high optical homogeneity of the films. The crystalline ß-Ta2O5 coating showed higher roughness and surface energy values than the other groups (P < .05) and was biocompatible. Compared with cpTi, the amorphous and crystalline tantalum oxide films did not increase bacterial adhesion (P > .05). By tailoring the deposition parameters, we synthetized a crystalline ß-Ta2O5 coating that improved titanium surface properties and positively affected cell spreading and morphology, making it a promising surface treatment for titanium-based implants.


Assuntos
Materiais Biocompatíveis/química , Tecnologia Biomédica/métodos , Tantálio/química , Animais , Linhagem Celular , Materiais Revestidos Biocompatíveis/química , Camundongos , Osteoblastos/citologia , Osteoblastos/ultraestrutura , Refratometria , Termodinâmica , Difração de Raios X
17.
Int J Nanomedicine ; 14: 1359-1383, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30863066

RESUMO

Slp forms a crystalline array of proteins on the outermost envelope of bacteria and archaea with a molecular weight of 40-200 kDa. Slp can self-assemble on the surface of liposomes in a proper environment via electrostatic interactions, which could be employed to functionalize liposomes by forming Slp-coated liposomes for various applications. Among the molecular characteristics, the stability, adhesion, and immobilization of biomacromolecules are regarded as the most meaningful. Compared to plain liposomes, Slp-coated liposomes show excellent physicochemical and biological stabilities. Recently, Slp-coated liposomes were shown to specifically adhere to the gastrointestinal tract, which was attributed to the "ligand-receptor interaction" effect. Furthermore, Slp as a "bridge" can immobilize functional biomacromol-ecules on the surface of liposomes via protein fusion technology or intermolecular forces, endowing liposomes with beneficial functions. In view of these favorable features, Slp-coated liposomes are highly likely to be an ideal platform for drug delivery and biomedical uses. This review aims to provide a general framework for the structure and characteristics of Slp and the interactions between Slp and liposomes, to highlight the unique properties and drug delivery as well as the biomedical applications of the Slp-coated liposomes, and to discuss the ongoing challenges and perspectives.


Assuntos
Tecnologia Biomédica/métodos , Sistemas de Liberação de Medicamentos , Lipossomos/química , Glicoproteínas de Membrana/química , Lipossomos/ultraestrutura , Eletricidade Estática
18.
Int J Nanomedicine ; 14: 1725-1736, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30880976

RESUMO

Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above.


Assuntos
Tecnologia Biomédica/métodos , Tamanho da Partícula , Titânio/farmacologia , Líquidos Corporais/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Humanos , Teste de Materiais , Nanoestruturas/química , Nanoestruturas/ultraestrutura , Molhabilidade
19.
Curr Med Sci ; 39(1): 1-6, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30868484

RESUMO

Mixed reality (MR) technology is a new digital holographic image technology, which appears in the field of graphics after virtual reality (VR) and augmented reality (AR) technology, a new interdisciplinary frontier. As a new generation of technology, MR has attracted great attention of clinicians in recent years. The emergence of MR will bring about revolutionary changes in medical education training, medical research, medical communication, and clinical treatment. At present, MR technology has become the popular frontline information technology for medical applications. With the popularization of digital technology in the medical field, the development prospects of MR are inestimable. The purpose of this review article is to introduce the application of MR technology in the medical field and prospect its trend in the future.


Assuntos
Tecnologia Biomédica/métodos , Assistência à Saúde/métodos , Educação Médica/métodos , Simulação por Computador , Holografia , Humanos , Imagem Tridimensional , Realidade Virtual
20.
BMC Med ; 17(1): 68, 2019 03 27.
Artigo em Inglês | MEDLINE | ID: mdl-30914045

RESUMO

Blockchain is a shared distributed digital ledger technology that can better facilitate data management, provenance and security, and has the potential to transform healthcare. Importantly, blockchain represents a data architecture, whose application goes far beyond Bitcoin - the cryptocurrency that relies on blockchain and has popularized the technology. In the health sector, blockchain is being aggressively explored by various stakeholders to optimize business processes, lower costs, improve patient outcomes, enhance compliance, and enable better use of healthcare-related data. However, critical in assessing whether blockchain can fulfill the hype of a technology characterized as 'revolutionary' and 'disruptive', is the need to ensure that blockchain design elements consider actual healthcare needs from the diverse perspectives of consumers, patients, providers, and regulators. In addition, answering the real needs of healthcare stakeholders, blockchain approaches must also be responsive to the unique challenges faced in healthcare compared to other sectors of the economy. In this sense, ensuring that a health blockchain is 'fit-for-purpose' is pivotal. This concept forms the basis for this article, where we share views from a multidisciplinary group of practitioners at the forefront of blockchain conceptualization, development, and deployment.


Assuntos
Tecnologia Biomédica , Redes de Comunicação de Computadores , Assistência à Saúde/tendências , Sistemas de Informação Administrativa , Informática Médica , Tecnologia Biomédica/métodos , Tecnologia Biomédica/organização & administração , Tecnologia Biomédica/tendências , Redes de Comunicação de Computadores/organização & administração , Redes de Comunicação de Computadores/normas , Redes de Comunicação de Computadores/provisão & distribução , Redes de Comunicação de Computadores/tendências , Data Warehousing/métodos , Data Warehousing/tendências , Assistência à Saúde/métodos , Assistência à Saúde/organização & administração , Processamento Eletrônico de Dados/métodos , Processamento Eletrônico de Dados/organização & administração , Processamento Eletrônico de Dados/tendências , Utilização de Equipamentos e Suprimentos/organização & administração , Utilização de Equipamentos e Suprimentos/tendências , Ensaios de Triagem em Larga Escala/normas , Humanos , Sistemas de Informação Administrativa/normas , Sistemas de Informação Administrativa/tendências , Informática Médica/métodos , Informática Médica/organização & administração , Informática Médica/tendências , Registros Médicos/normas
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