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1.
PLoS One ; 17(8): e0272720, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35939499

RESUMO

Maintaining body temperature in pediatric patients is critical, but it is often difficult to use currently accepted core temperature measurement methods. Several studies have validated the use of the SpotOn sensor for measuring core temperature in adults, but studies on pediatric patients are still lacking. The aim of this study was to investigate the accuracy of the SpotOn sensor compared with that of esophageal temperature measurement in pediatric patients intraoperatively. Children aged 1-8 years with American Society of Anesthesiology Physical Condition Classification I or II scheduled to undergo elective ear surgery for at least 30 min under general anesthesia were enrolled. Body core temperature was measured every 15 min after induction till the end of anesthesia with an esophageal probe, axillary probe, and SpotOn sensor. We included 49 patients, providing a total 466 paired measurements. Analysis of Pearson rank correlation between SpotOn and esophageal pairs showed a correlation coefficient (r) of 0.93 (95% confidence interval [CI] 0.92-0.94). Analysis of Pearson rank correlation between esophageal and axillary pairs gave a correlation coefficient (r) of 0.89 (95% CI 0.87-0.91). Between the SpotOn and esophageal groups, Bland-Altman analysis revealed a bias (SD, 95% limits of agreement) of -0.07 (0.17 [-0.41-0.28]). Between the esophageal and axillary groups, Bland-Altman analysis showed a bias (SD, 95% limits of agreement) of 0.45 (0.22 [0-0.89]). In pediatric patients during surgery, the SpotOn sensor showed high correlation and agreement with the esophageal probe, which is a representative core temperature measurement method.


Assuntos
Temperatura Corporal , Temperatura Alta , Adulto , Criança , Humanos , Monitorização Intraoperatória/métodos , Estudos Prospectivos , Tecnologia , Temperatura
2.
CBE Life Sci Educ ; 21(3): ar54, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35939528

RESUMO

Writing is an important skill for communicating knowledge in science, technology, engineering, and mathematics (STEM) and an aid to developing students' communication skills, content knowledge, and disciplinary thinking. Despite the importance of writing, its incorporation into the undergraduate STEM curriculum is uneven. Research indicates that understanding faculty beliefs is important when trying to propagate evidence-based instructional practices, yet faculty beliefs about writing pedagogies are not yet broadly characterized for STEM teaching at the undergraduate level. Based on a nationwide cross-disciplinary survey at research-intensive institutions, this work aims to understand the extent to which writing is assigned in undergraduate STEM courses and the factors that influence faculty members' beliefs about, and reported use of, writing-based pedagogies. Faculty attitudes about the effectiveness of writing practices did not differ between faculty who assign and do not assign writing; rather, beliefs about the influence of social factors and contextually imposed instructional constraints informed their decisions to use or not use writing. Our findings indicate that strategies to increase the use of writing need to specifically target the factors that influence faculty decisions to assign or not assign writing. It is not faculty beliefs about effectiveness, but rather faculty beliefs about behavioral control and constraints at the departmental level that need to be targeted.


Assuntos
Ciência , Estudantes , Humanos , Matemática , Ciência/educação , Ensino , Tecnologia/educação , Redação
3.
J Environ Public Health ; 2022: 8351868, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35942141

RESUMO

Vocal music teaching resource databases can support teachers' instruction and students' learning while also enhancing the school's ability to run a school because they are the main components of digital construction for vocal music teaching. Currently, system application is the main area of research for DM (data mining), which was previously focused on method discovery. A vocal music teaching resource base based on DM technology is created by using the theme as the central organising principle, processing, and organising the resources. Incorporate lesson planning, teaching, self-study, and output resource construction into a digital network resource application environment by using thematic resource development. Students' achievement data, personal basic information, and evaluation data are used for DM based on the enhanced Apriori algorithm in order to uncover hidden rules and find correlations between various factors. This information is then used to support decision-making. When the system load is 1, the results demonstrate that the Apriori algorithm's CPU waste rate and task success rate is 0.144 and 0.896, respectively. The study's conclusion demonstrates that integrating DM technology into the field of education is theoretically and practically possible and that there is significant room for future study and application in this area.


Assuntos
Música , Mineração de Dados , Humanos , Instituições Acadêmicas , Estudantes , Tecnologia
4.
Biomed Res Int ; 2022: 7117627, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35937386

RESUMO

To explore the clinical efficacy of microneedle injection combined with blood transfusion in the treatment of severe anemia complicated with vitiligo based on regenerative medical technology and provide the theoretical basis for the adoption of microneedle technology, 60 patients with severe anemia complicated with vitiligo were selected as research objects. With 15 patients in each group, they were randomly assigned to the control group (calcipotriol ointment external application), observation group A (external application of moist exposed burn ointment (MEBO), observation group B (external application of MEBO combined with blood transfusion), and observation group C (microneedle injection of MEBO combined with blood transfusion). Blood indexes and plaque recovery of patients in different periods were detected. The total protein (TP) content in group C (62.3 ± 3.3 g/L and 64.3 ± 2.88 g/L) was remarkably higher than that in the control group (51.3 ± 3.17 g/L and 52.4 ± 3.17 g/L) and group A (52.6 ± 2.91 g/L and 51.8 ± 2.98 g/L)) at the 5th and 7th weeks after the treatment (P < 0.05). The albumin (ALB) content in group C (42.9 ± 3.28 g/L and 45.3 ± 3.1 g/L) was signally higher than that in the control group (41.8 ± 3.44 g/L and 41.9 ± 3.23 g/L) and group A (41.3 ± 2.91 g/L and 42.1 ± 3.02 g/L) at the 5th and 7th weeks after the treatment, and the content was markedly higher than that in group B at 5th week (P < 0.05). The wound healing rates of group C at the 3rd, 5th, and 7th weeks after the treatment (38.44%, 56.6%, and 90.23%) were greatly higher than those of the control group, group A, and group B (P < 0.05). Besides, the wound healing rate of group B was higher than that of the control group and group A (40.3% and 75.8%) at the 5th and 7th weeks after the treatment (P < 0.05). To sum up, based on regenerative medical technology, microneedle injection (microneedling is a derma roller process that pricks the skin with minuscule needles. The goal of the treatment is to develop new collagen and skin tissue, resulting in skin that is smoother, firmer, and more toned) combined with blood transfusion had a good therapeutic effect on patients with severe anemia complicated with vitiligo, which could manifestly improve the blood indexes and skin plaques of patients, with a good clinical adoption effect.


Assuntos
Anemia , Vitiligo , Transfusão de Sangue , Humanos , Agulhas , Tecnologia , Resultado do Tratamento , Vitiligo/complicações , Vitiligo/terapia
5.
Biomater Adv ; 138: 212916, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35913255

RESUMO

Current drug screening approaches are incapable of fully detecting and characterizing drug effectiveness and toxicity of human cardiomyocytes. The pharmaceutical industry uses mathematical models, cell lines, and in vivo models. Many promising drugs are abandoned early in development, and some cardiotoxic drugs reach humans leading to drug recalls. Therefore, there is an unmet need to have more reliable and predictive tools for drug discovery and screening applications. Biofabrication of functional cardiac tissues holds great promise for developing a faithful 3D in vitro disease model, optimizing drug screening efficiencies enabling precision medicine. Different fabrication techniques including molding, pull spinning and 3D bioprinting were used to develop tissue-engineered heart chambers. The big challenge is to effectively organize cells into tissue with structural and physiological features resembling native tissues. Some advancements have been made in engineering miniaturized heart chambers that resemble a living pump for drug screening and disease modeling applications. Here, we review the currently developed tissue-engineered heart chambers and discuss challenges and prospects.


Assuntos
Impressão Tridimensional , Engenharia Tecidual , Descoberta de Drogas , Humanos , Miócitos Cardíacos , Tecnologia , Engenharia Tecidual/métodos
6.
Comput Intell Neurosci ; 2022: 3715730, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35909822

RESUMO

With the globalization of the world economy and the integration and heterogeneity of cultures, the collision between traditional settlements and local traditional culture, traditional culture and modern culture is gradually reduced. Traditional cultures, traditional settlements, and traditional architectural forms have gradually declined. Therefore, in the context of globalization, people are more concerned about how to recognize, understand, and inherit these traditions and traditional ways of life in the context of today's society and how to combine with the needs of contemporary communities to create an outdoor space suitable for human survival. However, due to the lack of research on traditional wind-heat conditions, there is no feasible evaluation method. Taking a typical village in Lianjiang County, Fuzhou as an example, various factors affecting wind-heat conditions in traditional villages are discussed in this paper. The CFD simulation technology is used to simulate and compare various types of settlements, and the wind and thermal environment around are compared and evaluated in detail and carried out in-depth research on it. By summarizing the general rules of natural ventilation of traditional residential buildings in Beigan Township, Lianjiang County, and Fuzhou, it is expected to be helpful to today's ecological construction. In order to construct a new type of energy-saving and land-saving community, some feasible methods and ideas are put forward to make it more realistic.


Assuntos
Temperatura Alta , Vento , Simulação por Computador , Computadores , Humanos , Tecnologia
7.
Comput Intell Neurosci ; 2022: 1310462, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35909871

RESUMO

The restoration design of Chinese traditional ancient buildings by VR technology can help to form a new media of ancient building culture and promote the process of digital protection in buildings. Studying the restoration design of Chinese traditional ancient buildings under VR technology can effectively protect the ancient building culture. This paper analyzes the unique design style of Chu architecture and integrates it into VR architecture restoration design. By taking the Zhanghua tower, a representative building in Chu, as an example, this paper expounds on the realization process of restoration design of ancient buildings in Chu. In addition, through 3 ds max and Unity3D platform, the plane shape description, virtual model construction, virtual model optimization, and virtual scene construction are completed, and the virtual space experience of Chu architecture is realized by using Virtools software.


Assuntos
Realidade Virtual , Software , Tecnologia
8.
Comput Intell Neurosci ; 2022: 4807953, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35909875

RESUMO

With the continuous development of sports software, college students, as the most advanced social new ideological group, running APP gradually enter the study and life of college students. Based on students' DM (data mining), this paper analyzes the influence of running apps on the improvement of college students' physique, chooses DT (decision tree) algorithm to construct the structure according to the characteristics of the data used, obtains students' DM model, and prunes it by using substitution error rate and PEP (pessimistic error pruning). The results show that the results of intra-group comparison among boys show that the scores of 1000 m in the intervention group have no obvious change compared with those before the intervention, and the difference is not statistically significant (P=0.516). The 800 m scores of girls in the intervention group were better than those in the control group, and the difference was statistically significant (P=0.03). The results of intra-group comparison showed that there was no significant difference in the scores of the intervention group before and after the intervention, and the difference was not statistically significant (P=0.32). After the experiment, the vital capacity scores of boys and girls in APP intervention group and control group were improved, with statistical significance (P < 0.05). The conclusion shows that running APP can improve students' speed level, cultivate students' endurance level, and improve students' physical health.


Assuntos
Corrida , Estudantes , Mineração de Dados , Feminino , Humanos , Masculino , Tecnologia
10.
Proc Natl Acad Sci U S A ; 119(32): e2123105119, 2022 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-35914160

RESUMO

As the workforce shifts to being predominantly hybrid and remote, how can companies help employees-particularly early-career women in science, technology, engineering, and mathematics (STEM) fields-develop greater confidence in their soft skills, shown to improve organizational retention? We evaluate the effects of an online longitudinal intervention to develop soft skills among early-career women employees at a North American biotechnology company during the height of the COVID-19 pandemic. Controlling for baseline levels collected immediately prior to nationwide lockdowns, we find that a 6-month online intervention increased early-career women's assessments of their soft skills at work by an average of 9% (P < 0.001), compared with a decrease of about 3.5% for a matched control group (P < 0.05), resulting in an average treatment effect of nearly 13% on the treated group. Furthermore, we find evidence that the intervention led to an increase in manager-assessed performance for early-career women relative to employees not in the intervention, and that overall, increased self-assessments of soft skill competencies were associated with greater odds of retention. Results show how employee soft skill development was affected by the pandemic and provide insights for a feasible and cost-effective method to train and engage a hybrid or fully remote workforce.


Assuntos
COVID-19 , Competência Profissional , Mulheres Trabalhadoras , Engenharia , Feminino , Humanos , Matemática , Ocupações , Pandemias , Ciência , Tecnologia
11.
Trials ; 23(1): 621, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35915473

RESUMO

BACKGROUND: Latinas are at increased risk for many lifestyle-related chronic diseases and are one of the least physically active populations in the US Innovative strategies are needed to help Latinas achieve the health benefits associated with physical activity (PA). This manuscript describes the study protocol of the Pasos Hacia La Salud II Study, which builds upon our previous research to test an enhanced individually-tailored, text-message and website-delivered, Spanish-language intervention (enhanced intervention), in comparison to the original web-based Pasos Hacia La Salud Intervention (original intervention). METHODS: Sedentary Latinas between the ages of 18-65 will be recruited and will complete an orientation and baseline assessments. Participants will be subsequently randomized to the original intervention, or the Enhanced Intervention, which has greater targeting of theoretical constructs such as self-efficacy, enjoyment, and social support, and which uses text messages and more dynamic and refined website features to encourage increased website use. Using a linear mixed effects regression model, we will simultaneously estimate the intervention effects on mean accelerometer-measured hours/week of moderate-to-vigorous PA (MVPA) at 6, 12, 18, and 24 months, with a subject-specific intercept (intent-to-treat sample). Change in self-reported MVPA, measured via the 7-day Physical Activity Recall, will be assessed as a secondary outcome using a similar model. We will investigate potential mediators of the intervention effect using a multiple mediation approach, and potential moderators by evaluating potential interactions. As an exploratory outcome, we will study the differences (among both study arms) in cost, in US dollars, per minute increases in weekly mean MVPA. DISCUSSION: The original Pasos PA intervention showed efficacy in helping Latinas increase PA; we expect the Enhanced Intervention to help a larger proportion of participants to increase and maintain their PA long term. This web- and text-based enhanced intervention could have great reach and dissemination potential, which could be capitalized on in the future to help to advance health equity. Adaptations made in response to the COVID-19 pandemic are also described in this manuscript. TRIAL REGISTRATION: Clinical Trial Number: NCT03491592 . First posted April 9, 2018.


Assuntos
COVID-19 , Promoção da Saúde , Adolescente , Adulto , Idoso , Exercício Físico/fisiologia , Feminino , Promoção da Saúde/métodos , Hispânico ou Latino , Humanos , Pessoa de Meia-Idade , Pandemias , Ensaios Clínicos Controlados Aleatórios como Assunto , Tecnologia , Adulto Jovem
12.
Neurosurg Focus ; 52(6): E2, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35921183

RESUMO

OBJECTIVE: "Join," an imaging technology-based telemedicine system, allows simultaneous radiological information sharing between physically remote institutions, virtually connecting advanced medical institutions and rural hospitals. This study aimed to elucidate the health economics effect of Join for neurological telemedicine in rural areas in Hokkaido, Japan. METHODS: Information concerning 189 requests for patient transfer from Furano Kyokai Hospital, a regional rural hospital, to Asahikawa Medical University Hospital (AMUH), an advanced academic medical institution, was retrospectively collected. The Join system was established between Furano Kyokai Hospital and AMUH in February 2019. Data collected from patients between April 2017 and December 2018 were included in the non-Join group, and those collected between February 2019 and October 2020 were included in the Join group. Clinical variables, reasons for patient transfer requests, duration of hospital stay, and medical costs per patient were analyzed between these two groups. Furthermore, clinical characteristics were compared between patients who were transferred and not transferred based on Join. RESULTS: More patients were discharged < 7 days after transfer to AMUH in the non-Join group compared with the Join group (p = 0.02). When focusing on the Join group, more patients who were not transferred were discharged < 1 week (p < 0.01). On the other hand, more patients required surgery (p = 0.01) when transferred. The ratio of patients whose medical cost was < USD5000 substantially decreased, from 33% for the non-Join group to 13% for the Join group. CONCLUSIONS: An imaging technology-based telemedicine system, Join, contributed to reducing unnecessary neuro-emergency patient transfer in a remote rural area, and telemedicine with an integrated smartphone system allowed medical personnel to effectively triage at a distance neuro-emergency patients requiring advanced tertiary care.


Assuntos
Serviços Médicos de Emergência , Telemedicina , Humanos , Transferência de Pacientes , Estudos Retrospectivos , Tecnologia
13.
Front Public Health ; 10: 941083, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35923977

RESUMO

With the advent of the era of artificial intelligence, speech recognition engine technology has a profound impact on social production, life, education, and other fields. Voice interaction is the most basic and practical type of human-computer interaction. To build an intelligent and automatic physical education teaching mode, this paper combines human-computer interaction based on speech recognition technology with physical education teaching. Students input through voice signals, and the system receives signals, analyzes signals, recognizes signals, and feeds back information to students in multiple forms. For the system to process the external speech signal, this paper uses the Mel cepstral coefficient algorithm to extract the speech information. By comparing the speech recognition rate and antinoise rate of Hidden Markov Model, Probabilistic Statistics Neural Network, and Hybrid Model (Hidden Markov and Rate Statistical Neural Network combination), the speech recognition engine uses the hybrid model, and its speech recognition rate is 98.3%, and the average antinoise rate can reach 85%. By comparing the human-computer interaction physical education teaching method with the traditional teaching method, the human-computer interaction method is superior to the traditional teaching method in the acquisition of physical knowledge, the acquisition of physical skills, the satisfaction of physical education courses and the ability of active learning. It effectively solves the drawbacks of traditional physical education and rationally uses human-computer interaction technology. On the basis of not violating physical education, realize the diversification of physical education, improve the quality of teaching, improve students' individual development and students' autonomous learning ability. Therefore, the combination of human-computer interaction and physical education based on recognition engine technology is the trend of today's physical education development.


Assuntos
Percepção da Fala , Fala , Inteligência Artificial , Computadores , Humanos , Educação Física e Treinamento , Tecnologia
14.
J Extra Corpor Technol ; 54(2): 105-106, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35928333

Assuntos
Tecnologia , Humanos
15.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 39(8): 824-828, 2022 Aug 10.
Artigo em Chinês | MEDLINE | ID: mdl-35929930

RESUMO

OBJECTIVE: To compare the performance of high-throughput sequencing technology in prenatal thalassemia screening in Zhuhai area through comparison with traditional methods. METHODS: A total of 1463 pregnant women were randomly selected. Following DNA extraction, high-throughput sequencing and conventional three-step thalassemia screening were carried out for each sample. Inconsistent results samples were validated by quantitative fluorescence PCR (QF-PCR) or Sanger sequencing. The results by the two methods were compared. RESULTS: Among the 1463 cases, 318 (21.74%) were detected by conventional method, which included 210 (14.35%) with α-thalassemia, 97 (6.63%) with ß-thalassemia, 11 (0.75%) with composite α- and ß-thalassemia. Meanwhile, 379 cases (25.91%) of thalassemia were detected by high-throughput sequencing, which included 260 (17.77%) with α-thalassemia, 107 (7.31%) with ß-thalassemia, 12 (0.82%) with composite α- and ß-thalassemia. Six one cases were missed by the conventional method, which yielded a missed diagnosis rate of 16.09%, including 50 cases of α- thalassemia,10 cases of ß-thalassemia, and 1 case of α-compound ß-thalassemia. No cases of thalassemia were missed by high-throughput sequencing, and 10 rare thalassemia genotypes were detected. CONCLUSION: High-throughput sequencing technology can improve the detection rate of thalassemia and reduce the missed diagnosis rate. It has a high application value in prenatal thalassemia screening in Zhuhai area and can more effectively prevent the birth of patients with severe thalassemia.


Assuntos
Talassemia alfa , Talassemia beta , China , Feminino , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Mutação , Gravidez , Diagnóstico Pré-Natal/métodos , Tecnologia , Talassemia alfa/diagnóstico , Talassemia alfa/genética , Talassemia beta/diagnóstico , Talassemia beta/genética
16.
PLoS One ; 17(8): e0271694, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35930587

RESUMO

Technological convergence is an important organizational innovation capability, essential for improving the core competitiveness of green and sustainable industries. However, studies have mainly focused on measuring technological convergence and have ignored the factors that affect the capabilities of such convergence capabilities. Thus, this study attempts to bridge this gap by providing an in-depth analysis of the impact of structural holes in inter-organizational technical cooperation networks. This technological convergence is studied from the perspective of a patent. It also considers the moderating effects of the degree of patent cooperation and the cooperation distance. It employs the social network theory to construct inter-organizational patent cooperation, and technological convergence networks, to facilitate the analysis of the effect of structural holes on such a convergence. It empirically examines 52 non-ferrous metal recycling organizations, with close patent cooperation. Accordingly, the structural hole constraint index by the inter-organizational patent cooperation network, shows a positive U-shaped curve relationship with technology convergence. Moreover, the degree of inter-organizational patent cooperation strengthens the positive U-shaped relationship, and the distance of cooperation weakens the influence of the structural hole constraint index on technology convergence. Therefore, managers and policymakers should encourage more industry-academia-research or patent alliances and designate policies to promote such cooperation.


Assuntos
Indústrias , Tecnologia , China , Metais
17.
BMC Complement Med Ther ; 22(1): 210, 2022 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-35932042

RESUMO

Diabetic nephropathy (DN) is one of the most serious complications of diabetes and the main cause of end-stage renal failure. Rhubarb is a widely used traditional Chinese herb, and it has exhibited efficacy in reducing proteinuria, lowering blood sugar levels and improving kidney function in patients with DN. However, the exact pharmacological mechanism by rhubarb improves DN remain unclear due to the complexity of its ingredients. Hence, we systematically explored the underlying mechanisms of rhubarb in the treatment of DN. We adopted a network pharmacology approach, focusing on the identification of active ingredients, drug target prediction, gene collection, Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes enrichment. Molecular docking technology was used to verify the binding ability between the main active compounds and central therapeutic targets, and screen out the core active ingredients in rhubarb for the treatment of DN. Finally, molecular dynamics simulation was performed for the optimal core protein-ligand obtained by molecular docking using GROMACS software. The network analysis identified 16 active compounds in rhubarb that were linked to 37 possible therapeutic targets related to DN. Through protein-protein interaction analysis, TP53, CASP8, CASP3, MYC, JUN and PTGS2 were identified as the key therapeutic targets. By validation of molecular docking, finding that the central therapeutic targets have good affinities with the main active compounds of rhubarb, and rhein, beta-sitosterol and aloe-emodin were identified as the core active ingredients in rhubarb for the treatment of DN. Results from molecular dynamics simulations showed that TP53 and aloe-emodin bound very stably with a binding free energy of - 26.98 kcal/mol between the two. The results of the gene enrichment analysis revealed that the PI3K-Akt signalling pathway, p53 signalling pathway, AGE-RAGE signalling pathway and MAPK signalling pathway might be the key pathways for the treatment of DN, and these pathways were involved in podocyte apoptosis, glomerular mesangial cell proliferation, inflammation and renal fibrosis. Based on the network pharmacology approach and molecular docking technology, we successfully predicted the active compounds and their respective targets. In addition, we illustrated the molecular mechanisms that mediate the therapeutic effects of rhubarb against DN. These findings provided an important scientific basis for further research of the mechanism of rhubarb in the treatment of DN.


Assuntos
Diabetes Mellitus , Nefropatias Diabéticas , Emodina , Rheum , Nefropatias Diabéticas/tratamento farmacológico , Humanos , Simulação de Acoplamento Molecular , Farmacologia em Rede , Fosfatidilinositol 3-Quinases/metabolismo , Rheum/química , Rheum/metabolismo , Tecnologia
18.
Anal Chim Acta ; 1221: 340112, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35934354

RESUMO

Oral squamous cell carcinoma (OSCC) is the well-known malignancy and poses a serious threat to human health with high morbidity and mortality. Early detection and treatment can improve the recovery rate and reduce complications of OSCC. Therefore, we designed a lateral flow strip biosensor platform (HRCA-strip) based on the cascade nucleic acid amplification technology (HRCA) for colorimetric analysis of OSCC-associated has-microRNA 31-5p (miRNA 31). In this work, the target miRNA 31 mediated the formation of the sandwich complex structure on the surface of magnetic beads (MBs). Then, the sandwich complex structure could activate cascade amplification reaction between hybridization chain reaction (HCR) and rolling-circle amplification (RCA) to generate numerous G-quadruplex structures. The G-quadruplex structures combined with hemin to form hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme (H/G-HRP mimic enzyme) which were enriched on the T-line and catalyzed the oxidation of chromogenic substrates to generate colorimetric signal on the strip. The HRCA-strip platform could achieve highly sensitive and specific miRNA 31 detection with the limit of detection (LOD) as low as 3.21 fM. Moreover, the designed HRCA-strip platform also enabled portable detection of miRNA 31 in clinical sample which might show good potential for early clinical diagnosis of OSCC.


Assuntos
Técnicas Biossensoriais , Carcinoma de Células Escamosas , DNA Catalítico , Quadruplex G , Neoplasias de Cabeça e Pescoço , MicroRNAs , Neoplasias Bucais , DNA Catalítico/química , Hemina/química , Peroxidase do Rábano Silvestre/química , Humanos , Limite de Detecção , MicroRNAs/análise , Neoplasias Bucais/diagnóstico , Neoplasias Bucais/genética , Técnicas de Amplificação de Ácido Nucleico , Carcinoma de Células Escamosas de Cabeça e Pescoço , Tecnologia
19.
Contrast Media Mol Imaging ; 2022: 2995464, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35935306

RESUMO

Our aim of this study was to observe and analyze the performance of the real-time fluorescence quantitative nucleic acid amplification detection of Mycobacterium tuberculosis/rifampicin resistance (GeneXpert MTB/RIF), gene chip technology, and modified Roche culture method in detecting MTB in sputum submitted for inspection and drug susceptibility. Patients with smear-negative suspected pulmonary TB (n = 120) in our hospital were enrolled in this study using a random number table, and sputum samples submitted for inspection were tested by the GeneXpert MTB/RIF, gene chip technology, and modified Roche culture method. With clinical diagnosis as the gold standard, the performance (mainly sensitivity and specificity) of the above three detection methods in the diagnosis of MTB was compared. Next, the drug susceptibility test (DST) was carried out on sputum samples, tested positive by the three methods. With the solid culture results as the evaluation criteria, the performance of the three detection methods in the diagnosis MTB and DST was compared. When compared with the modified Roche culture method, the GeneXpert MTB/RIF had the highest positive rate and a shorter overall test duration (P < 0.05). In contrast with the gene chip technology, the GeneXpert MTB/RIF exhibited higher sensitivity and negative predictive value (NPV) and lower specificity, accuracy, positive predictive value (PPV), and Kappa value (P < 0.05). According to analysis of the diagnostic performance of the three detection methods, GeneXpert MTB/RIF displayed the highest diagnostic sensitivity, ideal predictive values, and the highest similarity with clinical diagnosis in results (P < 0.05). The detection of susceptibility to isoniazid (INH) and RIF showed that the GeneXpert MTB/RIF and gene chip technology performed ideally in DST of MTB. In comparison with the modified Roche culture method, the GeneXpert MTB/RIF and gene chip technology have more prominent performance in detecting MTB and drug susceptibility. Besides, to further improve the accuracy of clinical diagnosis, various molecular biology detection methods can be combined to avoid delaying of the best time for the diagnosis and treatment of the disease.


Assuntos
Mycobacterium tuberculosis , Humanos , Mycobacterium tuberculosis/genética , Análise de Sequência com Séries de Oligonucleotídeos , Rifampina/farmacologia , Rifampina/uso terapêutico , Sensibilidade e Especificidade , Escarro/microbiologia , Tecnologia
20.
Contrast Media Mol Imaging ; 2022: 2634080, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35935324

RESUMO

To reveal the relationship between the expression and disease progression, the expression of long noncoding RNA ATB (lncRNA ATB) and related protein kinase 9 (Nek 9) in serum of breast cancer patients by real-time fluorescence quantitative PCR (Q-PCR) is analyzed. The patients treated in our hospital from April 2021 to February 2022 are selected and grouped into several groups. Among them, 73 patients diagnosed with breast cancer and 44 patients with benign breast disease are divided into groups A and B, respectively. In addition, 50 healthy subjects are chosen for group C. The expressions of lncRNA ATB and Nek9 in serum of patients are detected by Q-PCR, and the relationship between different pathological parameters and lncRNA ATB and Nek9 in group A is analyzed. Spearman correlation coefficient is used to evaluate the correlation between lncRNA ATB and Nek9 and disease. The experimental results show that lncRNA ATB and Nek9 in 3 groups are significantly different, and group A> group B> group C (all P < 0.05). The expression of lncRNA ATB and Nek9 is closely associated with lymph node metastasis and TNM stage in breast cancer patients (P < 0.05). Besides, the Spearman correlation coefficient shows that lncRNA ATB and Nek9 are positively correlated with breast cancer incidence (P < 0.05). It is evident that lncRNA ATB and Nek9 are expressed at high levels in breast cancer patients and are positively correlated with the occurrence and development of the disease.


Assuntos
Neoplasias da Mama , RNA Longo não Codificante , Neoplasias da Mama/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Quinases Relacionadas a NIMA/genética , Quinases Relacionadas a NIMA/metabolismo , Reação em Cadeia da Polimerase , Prognóstico , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Tecnologia
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