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1.
Colloids Surf B Biointerfaces ; 207: 112017, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34391169

RESUMO

In the past two decades, the increase in microbial resistance to conventional antimicrobials has spurred scientists around the world to search tirelessly for new treatments. Synthetic amino acid-based surfactants constitute a promising alternative to conventional antimicrobial compounds. In this work, two new cationic amino acid-based surfactants were synthesized and their physicochemical, antifungal and antibiofilm properties evaluated. The surfactants were based on phenylalanine-arginine (LPAM) and tryptophan-arginine (LTAM) and prepared from renewable raw materials using a simple chemical procedure. The critical micelle concentrations of the new surfactants were determined by conductivity and fluorescence. Micellization of LPAM and LTAM took place at 1.05 and 0.54 mM, respectively. Both exhibited good antifungal activity against fluconazole-resistant Candida spp. strains, with a low minimum inhibitory concentration (8.2 µg/mL). Their mechanism of action involves alterations in cell membrane permeability and mitochondrial damage, leading to death by apoptosis. Furthermore, when LPAM and LTAM were applied with Amphotericin B, a significant synergistic effect was observed against all the studied Candida strains. These new cationic surfactants are also able to disperse biofilms of Candida spp. at low concentrations. The results indicate that LPAM and LTAM have potential application to combat the advance of fungal resistance as well as microbial biofilms.


Assuntos
Antifúngicos , Fluconazol , Anfotericina B/farmacologia , Antifúngicos/farmacologia , Arginina , Biofilmes , Candida , Farmacorresistência Fúngica , Fluconazol/farmacologia , Testes de Sensibilidade Microbiana , Fenilalanina , Tensoativos/farmacologia , Triptofano
2.
J Biol Chem ; 297(2): 100940, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34237302

RESUMO

The severe acute respiratory syndrome coronavirus 2 envelope protein (S2-E) is a conserved membrane protein that is important for coronavirus (CoV) assembly and budding. Here, we describe the recombinant expression and purification of S2-E in amphipol-class amphipathic polymer solutions, which solubilize and stabilize membrane proteins, but do not disrupt membranes. We found that amphipol delivery of S2-E to preformed planar bilayers results in spontaneous membrane integration and formation of viroporin cation channels. Amphipol delivery of the S2-E protein to human cells results in plasma membrane integration, followed by retrograde trafficking to the trans-Golgi network and accumulation in swollen perinuclear lysosomal-associated membrane protein 1-positive vesicles, likely lysosomes. CoV envelope proteins have previously been proposed to manipulate the luminal pH of the trans-Golgi network, which serves as an accumulation station for progeny CoV particles prior to cellular egress via lysosomes. Delivery of S2-E to cells will enable chemical biological approaches for future studies of severe acute respiratory syndrome coronavirus 2 pathogenesis and possibly even development of "Trojan horse" antiviral therapies. Finally, this work also establishes a paradigm for amphipol-mediated delivery of membrane proteins to cells.


Assuntos
Membrana Celular/efeitos dos fármacos , Proteínas do Envelope de Coronavírus/metabolismo , Polímeros/farmacologia , Propilaminas/farmacologia , Tensoativos/farmacologia , Rede trans-Golgi/metabolismo , Membrana Celular/metabolismo , Proteínas do Envelope de Coronavírus/genética , Células HeLa , Humanos , Bicamadas Lipídicas/química , Bicamadas Lipídicas/metabolismo , Lisossomos/metabolismo , Polímeros/química , Propilaminas/química , Transporte Proteico , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Tensoativos/química
3.
Molecules ; 26(14)2021 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-34299397

RESUMO

We followed a comparative approach to investigate how heavy vacuum gas oil (HVGO) affects the expression of genes involved in biosurfactants biosynthesis and the composition of the rhamnolipid congeners in Pseudomonas sp. AK6U. HVGO stimulated biosurfactants production as indicated by the lower surface tension (26 mN/m) and higher yield (7.8 g/L) compared to a glucose culture (49.7 mN/m, 0.305 g/L). Quantitative real-time PCR showed that the biosurfactants production genes rhlA and rhlB were strongly upregulated in the HVGO culture during the early and late exponential growth phases. To the contrary, the rhamnose biosynthesis genes algC, rmlA and rmlC were downregulated in the HVGO culture. Genes of the quorum sensing systems which regulate biosurfactants biosynthesis exhibited a hierarchical expression profile. The lasI gene was strongly upregulated (20-fold) in the HVGO culture during the early log phase, whereas both rhlI and pqsE were upregulated during the late log phase. Rhamnolipid congener analysis using high-performance liquid chromatography-mass spectrometry revealed a much higher proportion (up to 69%) of the high-molecularweight homologue Rha-Rha-C10-C10 in the HVGO culture. The results shed light on the temporal and carbon source-mediated shifts in rhamonlipids' composition and regulation of biosynthesis which can be potentially exploited to produce different rhamnolipid formulations tailored for specific applications.


Assuntos
Proteínas de Bactérias/metabolismo , Gases/farmacologia , Glicolipídeos/biossíntese , Glicosiltransferases/metabolismo , Óleos Voláteis/farmacologia , Pseudomonas/metabolismo , Percepção de Quorum , Pseudomonas/efeitos dos fármacos , Pseudomonas/crescimento & desenvolvimento , Ramnose/metabolismo , Tensoativos/farmacologia , Volatilização
4.
Photochem Photobiol Sci ; 20(7): 939-953, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34255302

RESUMO

A novel tricationic Zn(II)phthalocyanine derivative, (NCH3)3ZnPc3+, was synthesized by ring expansion reaction of boron(III) [2,9(10),16(17)-trinitrosubphthalocyaninato]chloride. First, the reaction of this subphthalocyanine with 2,3-naphthalenedicarbonitrile and Zn(CH3COO)2 catalyzed by 8-diazabicyclo[5.4.0]undec-7-ene was used to obtain the A3B-type nitrophthalocyanine. After reduction of nitro groups with Na2S and exhaustive methylation of amino groups, (NCH3)3ZnPc3+ was formed in good yields. In addition, the tetracationic analog (NCH3)4ZnPc4+ was synthesized to compare their properties. The absorption and fluorescence spectra showed the Q-bands and the red emission, respectively, which are characteristic of the Zn(II)phthalocyanine derivatives in N,N-dimethylformamide. Furthermore, photodynamic activity sensitized by these compounds was studied in the presence of different molecular probes to sense the formation of reactive oxygen species. (NCH3)3ZnPc3+ efficiently produced singlet molecular oxygen and also it sensitized the formation of superoxide anion radical in the presence of NADH, while the photodynamic activity of (NCH3)4ZnPc4+ was very poor, possibly due to the partial formation of aggregates. Furthermore, the decomposition of L-tryptophan induced by (NCH3)3ZnPc3+ was mainly mediated by a type II mechanism. Antimicrobial photodynamic inactivation sensitized by these phthalocyanines was evaluated in Staphylococcus aureus, Escherichia coli, and Candida albicans, as representative microbial cells. In cell suspensions, (NCH3)3ZnPc3+ was rapidly bound to microbial cells, showing bioimages with red fluorescence emission. After 5 min of irradiation with visible light, (NCH3)3ZnPc3+ was able to completely eliminate S. aureus, E. coli and C. albicans, using 1.0, 2.5 and 5.0 µM phthalocyanine, respectively. In contrast, a low photoinactivation activity was found with (NCH3)4ZnPc4+ as a photosensitizer. Therefore, the amphiphilic tricationic phthalocyanine (NCH3)3ZnPc3+ is a promising photosensitizing structure for application as a broad-spectrum antimicrobial phototherapeutic agent.


Assuntos
Anti-Infecciosos/farmacologia , Indóis/farmacologia , Compostos Organometálicos/farmacologia , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Tensoativos/farmacologia , Anti-Infecciosos/química , Candida albicans/efeitos dos fármacos , Cátions/química , Cátions/farmacologia , Escherichia coli/efeitos dos fármacos , Indóis/química , Testes de Sensibilidade Microbiana , Compostos Organometálicos/química , Fármacos Fotossensibilizantes/química , Staphylococcus aureus/efeitos dos fármacos , Tensoativos/química
5.
J Nanosci Nanotechnol ; 21(12): 5945-5959, 2021 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-34229790

RESUMO

Zinc oxide nanoparticles were synthesized using different surfactants such as SDS, CTAB, Triton X-100, PVP K-30 and ethylene glycol. ZnO NPs were tested for antibacterial activity before and after calcination against different micro-organisms like E. coli and P. aeruginosa (Gram negative) as well as S. aureus and B. subtilis (Gram positive). Antibacterial activity was observed in SDScapped ZnO NPs only against B. subtilis. Antibacterial activity of ZnO-capped SDS was tested in a concentration range 0.625-10 mg/mL. Increased antibacterial activity was observed before calcination as compared to after calcination. Minimum concentration at which uncalcinated as well as calcinated SDS-capped ZnO NPs show antibacterial activity is 2.5 mg/mL and 5 mg/mL respectively. Non-antibacterial nature of ZnO NPs highlights its further use in drug delivery due to its inert nature, enhanced efficacy in association with therapeutic drugs as well as easy disposal.


Assuntos
Nanopartículas , Óxido de Zinco , Antibacterianos/farmacologia , Escherichia coli , Testes de Sensibilidade Microbiana , Staphylococcus aureus , Tensoativos/farmacologia , Óxido de Zinco/farmacologia
6.
Science ; 373(6554): 541-547, 2021 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-34326236

RESUMO

Repurposing drugs as treatments for COVID-19, the disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has drawn much attention. Beginning with sigma receptor ligands and expanding to other drugs from screening in the field, we became concerned that phospholipidosis was a shared mechanism underlying the antiviral activity of many repurposed drugs. For all of the 23 cationic amphiphilic drugs we tested, including hydroxychloroquine, azithromycin, amiodarone, and four others already in clinical trials, phospholipidosis was monotonically correlated with antiviral efficacy. Conversely, drugs active against the same targets that did not induce phospholipidosis were not antiviral. Phospholipidosis depends on the physicochemical properties of drugs and does not reflect specific target-based activities-rather, it may be considered a toxic confound in early drug discovery. Early detection of phospholipidosis could eliminate these artifacts, enabling a focus on molecules with therapeutic potential.


Assuntos
Antivirais/farmacologia , COVID-19/tratamento farmacológico , Reposicionamento de Medicamentos , Lipidoses/induzido quimicamente , Fosfolipídeos/metabolismo , SARS-CoV-2/efeitos dos fármacos , Células A549 , Animais , Antivirais/química , Antivirais/uso terapêutico , Antivirais/toxicidade , COVID-19/virologia , Cátions , Chlorocebus aethiops , Relação Dose-Resposta a Droga , Feminino , Humanos , Camundongos , Testes de Sensibilidade Microbiana , SARS-CoV-2/fisiologia , Tensoativos/química , Tensoativos/farmacologia , Tensoativos/toxicidade , Células Vero , Replicação Viral/efeitos dos fármacos
7.
Int J Nanomedicine ; 16: 3937-3999, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34140768

RESUMO

Surfactants, whose existence has been recognized as early as 2800 BC, have had a long history with the development of human civilization. With the rapid development of nanotechnology in the latter half of the 20th century, breakthroughs in nanomedicine and food nanotechnology using nanoparticles have been remarkable, and new applications have been developed. The technology of surfactant-coated nanoparticles, which provides new functions to nanoparticles for use in the fields of nanomedicine and food nanotechnology, is attracting a lot of attention in the fields of basic research and industry. This review systematically describes these "surfactant-coated nanoparticles" through various sections in order: 1) surfactants, 2) surfactant-coated nanoparticles, application of surfactant-coated nanoparticles to 3) nanomedicine, and 4) food nanotechnology. Furthermore, current progress and problems of the technology using surfactant-coated nanoparticles through recent research reports have been discussed.


Assuntos
Nanomedicina/métodos , Nanopartículas/química , Nanopartículas/uso terapêutico , Tensoativos/química , Animais , Encéfalo/efeitos dos fármacos , Sistemas de Liberação de Medicamentos , Alimentos , Humanos , Nanopartículas/administração & dosagem , Nanotecnologia/métodos , Tensoativos/farmacologia
8.
Biotechniques ; 71(1): 370-375, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34139879

RESUMO

Inactivation of SARS-CoV-2 virus is necessary to mitigate risk but may interfere with diagnostic assay performance. We examined the effect of heat inactivation on a prototype SARS-CoV-2 antigen immunoassay run on the ARCHITECT automated analyzer. Recombinant full-length SARS-CoV-2 nucleocapsid protein and virus lysate detection was reduced by 66 and 31%, respectively. Several nonionic detergents were assessed as inactivation alternatives based on infectivity in cultured Vero CCL81 cells. Incubation of SARS-CoV-2 in 0.1% Tergitol 15-S-9 for 10 min significantly reduced infectivity and increased the immunoassay signal for cultured lysate and patient specimens. Tergitol 15-S-9 can inactivate SARS-CoV-2 while preserving epitopes on the nucleocapsid protein for enhanced detection by immunoassay antibodies.


Assuntos
Teste para COVID-19/métodos , Poloxaleno/farmacologia , SARS-CoV-2/efeitos dos fármacos , SARS-CoV-2/imunologia , Inativação de Vírus/efeitos dos fármacos , Animais , Anticorpos Antivirais/efeitos dos fármacos , Anticorpos Antivirais/imunologia , COVID-19/diagnóstico , COVID-19/imunologia , Teste para COVID-19/normas , Células Cultivadas , Chlorocebus aethiops , Humanos , Imunoensaio/métodos , Imunoensaio/normas , Nucleocapsídeo/imunologia , Tensoativos/farmacologia , Células Vero
9.
ACS Appl Mater Interfaces ; 13(20): 23352-23368, 2021 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-33998809

RESUMO

Medical implant-associated infections resulting from biofilm formation triggered by unspecific protein adsorption are the prevailing cause of implant failure. However, implant surfaces rendered with multifunctional bioactive nanocoatings offer a promising alternative to prevent the initial attachment of bacteria and effectively interrupt biofilm formation. The need to research and develop novel and stable bioactive nanocoatings for medical implants and a comprehensive understanding of their properties in contact with the complex biological environment are crucial. In this study, we developed an aqueous stable and crosslinker-free polyelectrolyte-surfactant complex (PESC) composed of a renewable cationic polysaccharide, chitosan, a lysine-based anionic surfactant (77KS), and an amphoteric antibiotic, amoxicillin, which is widely used to treat a number of infections caused by bacteria. We successfully introduced the PESC as bioactive functional nanolayers on the "model" and "real" polydimethylsiloxane (PDMS) surfaces under dynamic and ambient conditions. Besides their high stability and improved wettability, these uniformly deposited nanolayers (thickness: 44-61 nm) with mixed charges exhibited strong repulsion toward three model blood proteins (serum albumin, fibrinogen, and γ-globulin) and their competitive interactions in the mixture in real-time, as demonstrated using a quartz crystal microbalance with dissipation (QCM-D). The functional nanolayers with a maximum negative zeta potential (ζ: -19 to -30 mV at pH 7.4), water content (1628-1810 ng cm-2), and hydration (low viscosity and elastic shear modulus) correlated with the mass, conformation, and interaction nature of proteins. In vitro antimicrobial activity testing under dynamic conditions showed that the charged nanolayers actively inhibited the growth of both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria compared to unmodified PDMS. Given the ease of fabrication of multifunctional and charged biobased coatings with simultaneous protein-repellent and antimicrobial activities, the limitations of individual approaches could be overcome leading to a better and advanced design of various medical devices (e.g., catheters, prosthetics, and stents).


Assuntos
Antibacterianos , Biofilmes/efeitos dos fármacos , Materiais Revestidos Biocompatíveis , Próteses e Implantes/microbiologia , Tensoativos , Antibacterianos/química , Antibacterianos/farmacologia , Quitosana/química , Quitosana/farmacologia , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Escherichia coli/efeitos dos fármacos , Interações Hidrofóbicas e Hidrofílicas , Lisina/química , Lisina/farmacologia , Nanomedicina , Nanoestruturas/química , Proteínas/química , Silício , Staphylococcus aureus/efeitos dos fármacos , Propriedades de Superfície , Tensoativos/química , Tensoativos/farmacologia
10.
Mar Drugs ; 19(5)2021 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-33946845

RESUMO

Catheter-associated urinary tract infections (CAUTIs) are among the leading nosocomial infections in the world and have led to the extensive study of various strategies to prevent infection. However, despite an abundance of anti-infection materials having been studied over the last forty-five years, only a few types have come into clinical use, providing an insignificant reduction in CAUTIs. In recent decades, marine resources have emerged as an unexplored area of opportunity offering huge potential in discovering novel bioactive materials to combat human diseases. Some of these materials, such as antimicrobial compounds and biosurfactants synthesized by marine microorganisms, exhibit potent antimicrobial, antiadhesive and antibiofilm activity against a broad spectrum of uropathogens (including multidrug-resistant pathogens) that could be potentially used in urinary catheters to eradicate CAUTIs. This paper summarizes information on the most relevant materials that have been obtained from marine-derived microorganisms over the last decade and discusses their potential as new agents against CAUTIs, providing a prospective proposal for researchers.


Assuntos
Antibacterianos/farmacologia , Organismos Aquáticos/metabolismo , Bactérias/efeitos dos fármacos , Infecções Relacionadas a Cateter/tratamento farmacológico , Cateteres de Demora/microbiologia , Infecção Hospitalar/tratamento farmacológico , Tensoativos/farmacologia , Cateterismo Urinário/instrumentação , Cateteres Urinários/microbiologia , Animais , Antibacterianos/isolamento & purificação , Bactérias/crescimento & desenvolvimento , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Infecções Relacionadas a Cateter/diagnóstico , Infecções Relacionadas a Cateter/microbiologia , Cateteres de Demora/efeitos adversos , Infecção Hospitalar/diagnóstico , Infecção Hospitalar/microbiologia , Desenho de Equipamento , Humanos , Metabolismo Secundário , Propriedades de Superfície , Tensoativos/isolamento & purificação , Cateterismo Urinário/efeitos adversos , Cateteres Urinários/efeitos adversos
11.
Food Chem ; 358: 129827, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33933977

RESUMO

Surface active agents derived from the non-toxic sweetener sucralose and fatty acids of different chain length were synthesized. Obtained compounds were characterized chemically and with regard to their properties as emulsifying agents, antimicrobial preservatives and fat-soluble sweeteners. Results show that sucralose-fatty acid esters are possible multi-purpose additives, compatible with both cosmetic and edible emulsions, as well as purely oil-based, waterless formulations. Their relative effectiveness in those applications varies, and is highly dependent on the fatty acid chain length, with hydrophobic/hydrophilic character strongly impacting both emulsifying and antimicrobial properties. While the structural differences between sucrose and sucralose proved to be enough to push all of the newly synthesized compounds out of the detergent/solubilizer category of surfactants, the retention of the substrate's high sweetness is an indication that non-bitter compounds with washing capabilities are possible to obtain.


Assuntos
Sacarose/análogos & derivados , Tensoativos/química , Tensoativos/farmacologia , Edulcorantes/química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Caprilatos/química , Emulsificantes/química , Emulsões , Ésteres/química , Ácidos Graxos/química , Interações Hidrofóbicas e Hidrofílicas , Testes de Sensibilidade Microbiana , Solubilidade , Relação Estrutura-Atividade , Sacarose/química , Tensoativos/síntese química , Edulcorantes/síntese química , Paladar
12.
Bioorg Med Chem ; 38: 116132, 2021 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-33872958

RESUMO

Since 2007, Metalloproteases (MMPs) have been considered potential targets for treating osteoarthritis (OA), for which the primary pathogenic event is the extensive degeneration of articular cartilage. MMP3 is an enzyme critical for these degenerative changes. However, problems of selectivity, low bioavailability and poor metabolic profile during clinical trials of MMPs inhibitors (MMPIs) led to limited beneficial effect and thus did not justify further pursuit of the clinical studies. In a previous work, a new alkyl derivative of hyaluronic acid (HA), HYADD4®, previously approved as intra-articular treatment for knee OA, was studied in vitro and in vivo as MMP3I. Molecular simulation studies confirmed the interaction between the alkyl side chain of this HA derivative and the additional S1' pocket of MMP3. However, the high MW and the polar HA backbone of HYADD4® imply a high desolvation energy cost, which can potentially decrease its inhibitory potency. In this study, a new class of MMP3Is based on a small peptide backbone (CGV) chemically derivatized with an alkyl chain was developed through interactive cycles of design, synthesis and screening, accompanied by computational evaluation and optimization. Two MMP3Is, e(I) and l(II), were selected because of their effective inhibitory activity (3.2 and 10.2 µM, respectively) and water solubility. Both MMPIs showed a broad range of inhibitory effects against almost all the MMPs tested. In an in vitro model of inflammatory OA, e(I) was the most effective MMPI: at the concentration of 93 µM, it reversed inflammatory outcomes. Moreover, because of its amphiphilic structure, the e(I) MMPI promoted stable micellar formulation at concentrations higher than 0.2 mg/mL in water. The findings were confirmed by TEM and Nile red staining analysis. Based on these results, the e(I) MMPI can be considered a good candidate for the intra-articular treatment of OA, and the micellar formulation of this peptide in an aqueous buffer can potentially increase the bioavailability and, thus, the efficacy of the MMPIs.


Assuntos
Inibidores Enzimáticos/farmacologia , Metaloproteinase 3 da Matriz/metabolismo , Osteoartrite do Joelho/tratamento farmacológico , Peptídeos/farmacologia , Tensoativos/farmacologia , Animais , Bovinos , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Humanos , Modelos Moleculares , Estrutura Molecular , Osteoartrite do Joelho/metabolismo , Peptídeos/síntese química , Peptídeos/química , Relação Estrutura-Atividade , Tensoativos/síntese química , Tensoativos/química
13.
Drug Discov Today ; 26(8): 1929-1935, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-33831583

RESUMO

The concept of going 'green' and 'cold' has led to utilizing renewable resources for the synthesis of microbial biosurfactants that are both patient and eco-friendly. In this review, we shed light on the potential and regulatory aspects of biosurfactants in pharmaceutical applications and how they can significantly contribute to novel concepts for the Coronavirus 2019 (COVID-19) vaccine and future treatment. We emphasize that more specific guidelines should be formulated to regulate the approval of biosurfactants for human use. It is also crucial to implement a risk-based approach from the early research and development (R&D) phase in addition to establishing more robust standardized techniques and assays to evaluate the characteristics of biosurfactants.


Assuntos
Antivirais/farmacologia , Vacinas contra COVID-19/farmacologia , COVID-19 , Descoberta de Drogas , SARS-CoV-2 , Tensoativos/farmacologia , COVID-19/tratamento farmacológico , COVID-19/prevenção & controle , Descoberta de Drogas/métodos , Descoberta de Drogas/tendências , Controle de Medicamentos e Entorpecentes/organização & administração , Fenômenos Ecológicos e Ambientais , Humanos , Nanoestruturas , Preparações Farmacêuticas/classificação , SARS-CoV-2/efeitos dos fármacos , SARS-CoV-2/fisiologia
14.
Arch Microbiol ; 203(6): 3389-3397, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33884457

RESUMO

Cleaning products containing live bacteria that form spores of Bacillus spp. as active substances are becoming increasingly common in probiotic cleaner formulation. The quality of cleaning performance for the production of probiotic cleaners does not only depend on the potential of the bacterial strains used, but also on the chemical components of the formulations. In this study, the surfactants and other additives were investigated as biocidal or bacteriostatic against B. subtilis, and the viability of B. subtilis was examined at different pH ranges for microbial cleaner formulation. As a result, it was discovered that the B. subtilis, which can be used in the microbial cleaner formulation, shows higher growth and viability at the neutral pH, and it passes into the death phase at pH 3. According to antagonistic activity results, the Gram-positive S. aureus and K. pneumoniae were the most sensitive bacteria while B. cereus was the most resistant bacteria. The anionic surfactants such as linear alkylbenzene sulfonic acid and sodium lauryl ether sulfate act as bacteriostatic on Bacillus spp. and do not cause cell death. In the view of these results, the usage of appropriate bacterial cultures and the correct stabilization of the formulations are also critical elements in the development of microbial cleaner formulations.


Assuntos
Bacillus subtilis , Probióticos , Tensoativos , Antibacterianos/farmacologia , Bacillus subtilis/efeitos dos fármacos , Viabilidade Microbiana/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Tensoativos/farmacologia
15.
Bioelectrochemistry ; 140: 107809, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33862547

RESUMO

SRB is one of the main bacteria causing marine microbial corrosion. In order to reduce the loss of microbial corrosion, a Gemini surfactant (12-B-12) containing semi-rigid spacer was used to investigate the anti-bacterial and anti-corrosion performances of carbon steel in simulated seawater by weight-loss test, electrochemical method and surface morphology analysis. The results showed that the inhibition efficiency of 0.01 mM 12-B-12 was as high as 98.3% after 30 days of incubation in simulated seawater with SRB, and the planktonic and sessile SRB on the carbon steel surface can be reduced to undetectable level. Quantum chemical calculation and molecular dynamics simulation were used to study the structure-activity relationship.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Calcitriol/análogos & derivados , Carbono/química , Água do Mar/química , Água do Mar/microbiologia , Aço/química , Antibacterianos/química , Calcitriol/química , Corrosão , Eletroquímica , Fenômenos Mecânicos , Conformação Molecular , Simulação de Dinâmica Molecular , Relação Estrutura-Atividade , Tensoativos/química , Tensoativos/farmacologia
16.
J Laryngol Otol ; 135(6): 529-532, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33843502

RESUMO

OBJECTIVE: To investigate the effects of nasal irrigation with sodium hyaluronate and surfactant solutions on mucociliary clearance time in patients with mild persistent allergic rhinitis. METHODS: A total of 120 patients diagnosed with mild persistent allergic rhinitis were enrolled in this prospective study. The patients were allocated randomly to the surfactant, sodium hyaluronate or isotonic saline (as a control) nasal irrigation group. The mucociliary clearance times and improvements in mucociliary clearance times were compared. RESULTS: Improvements in mean mucociliary clearance time were significantly greater in the surfactant and sodium hyaluronate groups than in the control group (p < 0.01). The mean post-treatment mucociliary clearance time of the surfactant group was significantly lower than that of the control (p < 0.001) and sodium hyaluronate groups (p = 0.03). CONCLUSION: Surfactant and sodium hyaluronate nasal irrigation solutions may both be used as adjunctive treatments for allergic rhinitis. Surfactant nasal irrigation resulted in better mucociliary clearance times.


Assuntos
Ácido Hialurônico/farmacologia , Ácido Hialurônico/uso terapêutico , Depuração Mucociliar/efeitos dos fármacos , Lavagem Nasal , Rinite Alérgica/terapia , Tensoativos/farmacologia , Tensoativos/uso terapêutico , Adolescente , Adulto , Idoso , Terapia Combinada , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Rinite Alérgica/fisiopatologia , Índice de Gravidade de Doença , Método Simples-Cego , Adulto Jovem
17.
Int J Mol Sci ; 22(7)2021 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-33804887

RESUMO

Ultrashort cationic lipopeptides (USCLs) and gemini cationic surfactants are classes of potent antimicrobials. Our recent study has shown that the branching and shortening of the fatty acids chains with the simultaneous addition of a hydrophobic N-terminal amino acid in USCLs result in compounds with enhanced selectivity. Here, this approach was introduced into arginine-rich gemini cationic surfactants. l-cystine diamide and l-lysine amide linkers were used as spacers. Antimicrobial activity against planktonic and biofilm cultures of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) strains and Candida sp. as well as hemolytic and cytotoxic activities were examined. Moreover, antimicrobial activity in the presence of human serum and the ability to form micelles were evaluated. Membrane permeabilization study, serum stability assay, and molecular dynamics were performed. Generally, critical aggregation concentration was linearly correlated with hydrophobicity. Gemini surfactants were more active than the parent USCLs, and they turned out to be selective antimicrobial agents with relatively low hemolytic and cytotoxic activities. Geminis with the l-cystine diamide spacer seem to be less cytotoxic than their l-lysine amide counterparts, but they exhibited lower antibiofilm and antimicrobial activities in serum. In some cases, geminis with branched fatty acid chains and N-terminal hydrophobic amino acid resides exhibited enhanced selectivity to pathogens over human cells.


Assuntos
Peptídeos Catiônicos Antimicrobianos/síntese química , Biofilmes/efeitos dos fármacos , Lipoproteínas/síntese química , Tensoativos/síntese química , Motivos de Aminoácidos , Peptídeos Catiônicos Antimicrobianos/farmacologia , Arginina/química , Candida/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , Cistina/química , Enterobacteriaceae/efeitos dos fármacos , Ácidos Graxos/química , Hemólise , Interações Hidrofóbicas e Hidrofílicas , Lipoproteínas/farmacologia , Lisina/química , Micelas , Tensoativos/farmacologia
18.
Can J Vet Res ; 85(2): 151-155, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33883824

RESUMO

Disinfection is key for controlling microbial contamination and ensuring the safe production of milk and dairy products. In this study, we developed a new disinfection method using quaternary ammonium surfactant N-dodecyl-2-(pyridin-1-yl) acetamide chloride as the main component to form a bactericidal complex with either chlorhexidine acetate or glutaraldehyde, and we evaluated the bactericidal effects, safety, and clinical application value of the compound disinfectants. An in vivo acute oral toxicity assay in mice showed an LD50 > 5000 mg/kg body weight without abnormality in pathological tissue sections. Comparison with commercially available products also showed that they have outstanding bactericidal effects. Clinical trials proved that the compound disinfectants have excellent bactericidal effects on the air and ground of the dairy farm and on the skin of cattle, especially in a dairy farm environment. Our findings confirm that the new compound disinfectants have excellent bactericidal performance and are safe to use as disinfectants to prevent mastitis and contamination of the cattle farm environment.


Assuntos
Bovinos , Laticínios/microbiologia , Desinfetantes/farmacologia , Microbiologia de Alimentos , Compostos de Amônio Quaternário/farmacologia , Animais , Bactérias/efeitos dos fármacos , Indústria de Laticínios , Microbiologia Ambiental , Fazendas , Feminino , Camundongos , Compostos de Amônio Quaternário/toxicidade , Tensoativos/farmacologia , Tensoativos/toxicidade
19.
PLoS One ; 16(4): e0249796, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33848316

RESUMO

The Japanese Ministry of Health requires large-scale cooking facilities to use sodium hypochlorite aqueous solution (HYP) on food preparation tools, equipment, and facilities to prevent secondary contamination. This study aimed to compare the disinfecting effect of HYP and surfactant using adenosine triphosphate (ATP) swab testing on large-scale equipment and facilities that could not be disassembled and disinfected in hospital kitchen. From May 2018 to July 2018, ATP swab tests were performed on the following six locations in the Shizuoka Cancer Centre Dietary Department Kitchen: cooking counter, mobile cooking counter, refrigerator handle, conveyor belt, tap handle, and sink. Six relative light unit (RLU) measurements were taken from each location. The log10 values of the RLU measurements were evaluated by dividing the samples into two groups: the control group (surfactant followed by HYP swabbing) and the HYP group (HYP swabbing only). The results showed that the RLUs (log10 values) in both the groups improved after disinfection (p<0.05), except for the RLUs (log10 values) of the mobile cooking counter, tap handle, and sink in the control group after the HYP swab. The changes in the RLU (log10 value) did not differ between the two groups for all locations of the kitchen. Hence, HYP swabbing of large-scale equipment and facilities provides the same level of disinfection as surfactants and can be as beneficial.


Assuntos
Trifosfato de Adenosina/análise , Desinfecção/métodos , Indústria Alimentícia/normas , Medições Luminescentes/métodos , Hipoclorito de Sódio/farmacologia , Tensoativos/farmacologia , Desinfetantes/farmacologia , Indústria Alimentícia/métodos , Hospitais , Humanos
20.
J Oleo Sci ; 70(4): 509-519, 2021 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-33692240

RESUMO

Softening agents, when applied in appropriate amounts, can impart softness to fabrics, particularly cotton towels, such that improved comfort and feel can be achieved when using the fabrics. On the other hand, water absorbency, which is commonly regarded as the mark of high-quality cotton products, significantly decreases when any of the currently existing softeners is used. To date, when a softener is used on cotton fabrics, there is a trade-off between excellent softness and high-water absorbency. In our research, we introduced a new sensory evaluation indicator called the "water wiping-off feeling ratio" which looks primitive but shows high correlation with our actual feel over any other existing indicators. Furthermore, we developed a new method and model to overcome the above-mentioned trade-off, involving the use of small particles with a hydrophilic surface together with the softener. Inspired by the theory of fractal geometry and the combination of models/equations by Cassie, Baxter, and Wenzel, the idea of adding new convex hydrophilic domains onto the surface of cotton fibers along with the softening agent was conceived. Finally, we successfully improved the wiping-off feel without decreasing the softness, i.e., we developed a strategy to overcome the above-mentioned trade-off in softener-treated fabrics that has proven challenging thus far.


Assuntos
Absorção Fisico-Química/efeitos dos fármacos , Fibra de Algodão , Interações Hidrofóbicas e Hidrofílicas , Tensoativos/química , Tensoativos/farmacologia , Água , Compostos Orgânicos/química , Compostos Orgânicos/farmacologia
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