Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 12.534
Filtrar
1.
J Agric Food Chem ; 67(37): 10361-10372, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31487173

RESUMO

Alkyl di-/tri-/tetraoxyethyl ß-d-xylopyranosides as derivatives of alkyl xylosides are a class of non-ionic sugar-based surfactants. They were stereoselectively synthesized by the Helferich method. Their properties including hydrophilic-lipophilic balance number, water solubility, surface property, foam property, emulsifying property, and thermotropic liquid crystal property were mainly investigated. The results showed that their water solubility decreased with increasing the alkyl chain length and increasing the number of the oligooxyethyl fragment. The critical micelle concentration had a monotonous decreasing trend with increasing the alkyl chain length. Nonyl di-/tri-/tetraoxyethyl ß-d-xylopyranosides [-(OCH2CH2)m-, where m = 2, 3, and 4] exhibited the most excellent foaming ability and foam stability. In the n-octane/water system, dodecyl tetraoxyethyl ß-d-xylopyranosides and tetradecyl tetraoxyethyl ß-d-xylopyranosides had the strongest emulsion ability. In addition, some alkyl di-/tri-/tetraoxyethyl ß-d-xylopyranosides had thermotropic liquid crystal properties. Such sugar-based surfactants, alkyl di-/tri-/tetraoxyethyl ß-d-xylopyranosides, will be expected to develop for a variety of practical application.


Assuntos
Glicosídeos/química , Tensoativos/química , Glicosídeos/síntese química , Interações Hidrofóbicas e Hidrofílicas , Solubilidade , Propriedades de Superfície , Tensoativos/síntese química
2.
Chem Commun (Camb) ; 55(72): 10654-10664, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31418758

RESUMO

Some host-guest complexes of cucurbit[n]uril (CB[n]) host molecules act as supramolecular amphiphiles (SAs), which hierarchically self-assemble into various nanomaterials such as vesicles, micelles, nanorods, and nanosheets in water. The structures and functions of the nanomaterials can be controlled by supramolecular engineering of the host-guest complexes. In addition, functionalization at the periphery of CB[6] and CB[7] generates CB[n]-based molecular amphiphiles (MAs) that can also self-assemble into vesicles or micelle-like nanoparticles in water. Taking advantage of the molecular cavities of CBs and their strong guest recognition properties, the surface of the self-assembled nanomaterials can be easily decorated with various functional tags in a non-covalent manner. In this feature article, the two types (SAs and MAs) of CB-based amphiphiles, their self-assemblies and their applications for nanotherapeutics and theranostics are presented with future perspectives.


Assuntos
Antibióticos Antineoplásicos/farmacologia , Hidrocarbonetos Aromáticos com Pontes/farmacologia , Doxorrubicina/farmacologia , Nanoestruturas/química , Tensoativos/farmacologia , Antibióticos Antineoplásicos/química , Hidrocarbonetos Aromáticos com Pontes/química , Proliferação de Células/efeitos dos fármacos , Doxorrubicina/química , Células HeLa , Humanos , Células KB , Tensoativos/química
3.
Int J Nanomedicine ; 14: 5381-5396, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31409994

RESUMO

Background: Tacrolimus (TCR), also known as FK-506, is a biopharmaceutics classification system (BCS) class II drug that is insoluble in water because of its high log P values. After dermal application, TCR remains in the stratum corneum and passes through the skin layers with difficulty. Purpose: The objectives of this study were to develop and evaluate solid lipid nanoparticles (SLNs) with thermosensitive properties to improve penetration and retention. Methods: We prepared TCR-loaded thermosensitive solid lipid nanoparticles (TCR-SLNs) with different types of surfactants on the shell of the particle, which conferred the advantages of enhancing skin permeation and distribution. We also characterized them from a physic point of view and performed in vitro and in vivo evaluations. Results: The TCR contained in the prepared TCR-SLN was in an amorphous state and entrapped in the particles with a high loading efficiency. The assessment of ex vivo skin penetration using excised rat dorsal skin showed that the TCR-SLNs penetrated to a deeper layer than the reference product (0.1% Protopic®). In addition, the in vivo skin penetration test demonstrated that TCR-SLNs delivered more drug into deeper skin layers than the reference product. FT-IR images also confirmed drug distribution of TCR-SLNs into deeper layers of the skin. Conclusion: These results revealed the potential application of thermosensitive SLNs for the delivery of difficult-to-permeate, poorly water-soluble drugs into deep skin layers.


Assuntos
Derme/metabolismo , Lipídeos/química , Nanopartículas/química , Tacrolimo/farmacologia , Temperatura Ambiente , Administração Cutânea , Animais , Varredura Diferencial de Calorimetria , Derme/efeitos dos fármacos , Liberação Controlada de Fármacos , Irritantes/toxicidade , Nanopartículas/ultraestrutura , Tamanho da Partícula , Coelhos , Ratos Sprague-Dawley , Absorção Cutânea/efeitos dos fármacos , Testes Cutâneos , Espectroscopia de Infravermelho com Transformada de Fourier , Tensoativos/química , Difração de Raios X
4.
J Cosmet Sci ; 70(3): 127-136, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31398101

RESUMO

The impact that rhamnolipid (RL) and sophorolipid (SL) biosurfactants has on solution surface activity when used in conjunction with the commercially important zwitterionic surfactant cocamidopropyl betaine (CAPB) is highlighted for the first time through surface tension and surface rheology measurements on binary and ternary mixtures of these surfactants. It was observed that in both the binary (CAPB/RL) and the ternary (CAPB/RL/SL) mixtures, RL tends to dominate at the air-water interface and primarily control both surface tension and surface elasticity behavior. Significant reduction of surface tension and enhancement of surface elasticity is observed as a result of the competitive adsorbtion/dominance of the RL at the air-water interface and this leads to performance enhancements in terms of foam stability.


Assuntos
Micelas , Tensoativos/química , Reologia , Tensão Superficial , Água
5.
Phys Chem Chem Phys ; 21(30): 16706-16717, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31321392

RESUMO

Herein, for the first time the complexation ability of a homological series of triphenylphosphonium surfactants (TPPB-n) toward DNA decamers has been explored. Formation of lipoplexes was confirmed by alternative techniques, including dynamic light scattering, indicating the occurrence of nanosized complexes (ca. 100-150 nm), and monitoring the charge neutralization of nucleotide phosphate groups and the fluorescence quenching of dye-intercalator ethidium bromide. The complexation efficacy of TPPB-surfactants toward an oligonucleotide (ONu) is compared with that of reference cationic surfactants. Strong effects of the alkyl chain length and the structure of the head group on the surfactant/ONu interaction are revealed, which probably occur via different mechanisms, with electrostatic and hydrophobic forces or intercalation imbedding involved. Phosphonium surfactants are shown to be capable of disordering lipid bilayers, which is supported by a decrease in the temperature of the main phase transition, Tm. This effect enhances with an increase in the alkyl chain length, indicating the integration of TPPB-n with lipid membranes. This markedly differs from the behavior of typical cationic surfactant cetyltrimethylammonium bromide, which induces an increase in the Tm value. It was demonstrated that the cytotoxicity of TPPB-n in terms of the MTT-test on a human cell line 293T nonmonotonically changes within the homological series, with the highest cytotoxicity exhibited by the dodecyl and tetradecyl homologs.


Assuntos
DNA/química , Bicamadas Lipídicas/química , Ácidos Nucleicos/química , Tensoativos/química , Membrana Celular/efeitos dos fármacos , Células HEK293 , Humanos , Tensoativos/toxicidade
6.
Chem Commun (Camb) ; 55(59): 8556-8559, 2019 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-31271173

RESUMO

Non-hydrated organic solutions of a diphenylalanine amphiphile blocked at the C-terminus with a fluorenylmethyl ester and stabilized at the N-terminus with a trifluoroacetate have been used to prepare amyloid fibrils. The solvent used to prepare the stock solution together with the co-solvent added enables regulation of the characteristics of the fibrils, which is important for their use in technological applications.


Assuntos
Amiloide/síntese química , Proteínas Amiloidogênicas/química , Dipeptídeos/química , Tensoativos/química , Teoria da Densidade Funcional , Dimetilformamida/química , Metanol/química , Propanóis/química , Conformação Proteica em Folha beta , Multimerização Proteica , Teoria Quântica
7.
Chem Commun (Camb) ; 55(57): 8227-8230, 2019 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-31268107

RESUMO

An original family of multivalent vectors encompassing gemini and facial amphiphilicity, namely cationic Siamese twin surfactants, has been prepared from the disaccharide trehalose; molecular engineering lets us modulate the self-assembling properties and the topology of the nanocomplexes with plasmid DNA for efficient gene delivery in vitro and in vivo.


Assuntos
Nanoestruturas/química , Plasmídeos/química , Tensoativos/química , Transfecção/métodos , Trealose/química , Animais , Linhagem Celular , Cercopithecus aethiops , Humanos , Camundongos , Plasmídeos/metabolismo
8.
J Agric Food Chem ; 67(29): 8197-8204, 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31287317

RESUMO

Potato pectin contains some proteinaceous components and exhibits emulsifying and emulsion stabilizing abilities. The objective of this study was to elucidate the effect of the pectin moiety of the pectin-protein conjugate present in pectic extracts from potato tubers on their interfacial properties. Potato pectin was extracted from highly purified cell wall materials to avoid the contamination of unconjugated proteins. The abilities of the potato pectin to adsorb to graphite surfaces and to resist surfactant-induced competitive displacement from the air-water interface were investigated using atomic force microscopy. The pectin moiety of the potato pectin was capable of adsorbing to graphite surfaces even after alkali treatment. Furthermore, the potato pectin exhibited enhanced resistance to surfactant-induced competitive displacement from the interface as a result of the formation of network structures of self-assembled pectin moieties at the interface. The present results suggest the importance of the pectin moiety with regard to the interfacial properties of potato pectin.


Assuntos
Pectinas/química , Extratos Vegetais/química , Proteínas de Plantas/química , Solanum tuberosum/química , Tensoativos/química , Ar/análise , Tubérculos/química , Água/química
9.
Carbohydr Polym ; 219: 162-171, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31151513

RESUMO

Rheological behaviors of microbial polysaccharides with different substituents in aqueous solutions have been systematically investigated. Both the saccharide side chains and acetyl substituents improve the gelation of welan gum (WG), diutan gum (DG) and gellan gum (GG) in pure water at 25 °C. For the polysaccharides with saccharide side chains (WG and DG), the relationship between the apparent viscosity and concentration conforms to the linear equation, while that of the polysaccharide with acetyl (GG) is exponential. More importantly, the roles of substituents on the stability of the molecular conformation of polysaccharides are significantly depended on the surrounding environment. Disaccharide side chains promote the stability of helical conformation and gel aggregates of GG at high temperature (85 °C) or in the presence of inorganic salts with the ionic strength of 2.0 mol L-1. The stability of gel structure containing acetyl (GG) shows higher temperature/salt sensitivity. Additionally, deacylated gellan gum (GG(d)) solutions transform into hydrogels in the presence of dodecyltrimethylammonium bromide (DTAB). This study will help to obtain a better understanding on the rheological properties of polysaccharides with respect to the conformation and applications.


Assuntos
Bactérias/metabolismo , Hidrogéis/química , Polissacarídeos Bacterianos/química , Reologia , Tensoativos/química , Ácido Acético/química , Concentração Osmolar , Sais/química , Soluções/química , Temperatura Ambiente , Viscosidade
10.
Pharm Res ; 36(8): 122, 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31218556

RESUMO

PURPOSE: A non-propellant based foam (NPF) system was developed incorporating the antibiotics, pectin capped green nano-silver and sulfadiazine (SD) for the topical treatment of burn wounds as a convenient alternative to the existing therapies. METHODS: NPF were prepared using various surfactants and oils forming a nanoemulsion. Anti-microbial studies by resazurin microtitre assay, ex vivo diffusion, in vivo skin permeation and deposition studies, and acute irritation studies were carried out. NPF was applied onto secondary thermal wounds manifested on mice models followed by macroscopic and histological examinations. RESULTS: NPF had an average globule size of <75 nm. The viscosity was ~10 cP indicating the feasibility of expulsion from the container upon actuation. With no skin irritation, the foams showed a higher skin deposition of SD. A high contraction and an evident regeneration of the skin tissue upon treatment with NPF indicated a good recovery from the thermal injury was apparent from the histology studies. CONCLUSION: NPF represents an alternative topical formulation that can be employed as a safe and effective treatment modality for superficial second degree (partial thickness) burn wounds. With a minimal requirement of mechanical force, the no-touch application of NPF makes it suitable for sensitive and irritant skin surfaces.


Assuntos
Antibacterianos/farmacologia , Queimaduras/tratamento farmacológico , Nanopartículas Metálicas/química , Prata/química , Sulfadiazina/farmacologia , Cicatrização/efeitos dos fármacos , Administração Tópica , Animais , Antibacterianos/administração & dosagem , Queimaduras/patologia , Queimaduras/fisiopatologia , Composição de Medicamentos/métodos , Quimioterapia Combinada , Emulsões , Escherichia coli/efeitos dos fármacos , Química Verde , Humanos , Masculino , Camundongos , Óleos/química , Tamanho da Partícula , Permeabilidade , Pele/efeitos dos fármacos , Pele/patologia , Pele/fisiopatologia , Staphylococcus aureus/efeitos dos fármacos , Sulfadiazina/administração & dosagem , Tensoativos/química
11.
Int J Nanomedicine ; 14: 3601-3613, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31190814

RESUMO

Introduction: Kolliphor® EL (K-EL) is among the most useful surfactants in the preparation of emulsions. However, it is associated with low hydrophobic drug loading in the resulting emulsified formulation. Methods: In this study, a formulation for intranasal administration of butylidenephthalide (Bdph), a candidate drug against glioblastoma (GBM), was prepared. Physical characteristics of the formulation such as particle size, zeta potential, conductivity, and viscosity were assessed, as well as its cytotoxicity and permeability, in order to optimize the formulation and improve its drug loading capacity. Results: The optimized formulation involved the integration of polyethylene glycol 400 (PEG 400) in K-EL to encapsulate Bdph dissolved in dimethyl sulfoxide (DMSO), and it exhibited higher drug loading capacity and drug solubility in water than the old formulation, which did not contain PEG 400. Incorporation of PEG 400 as a co-surfactant increased Bdph loading capacity to up to 50% (v/v), even in formulations using Kolliphor® HS 15 (K-HS15) as a surfactant, which is less compatible with Bdph than K-EL. The optimized Bdph formulation presented 5- and 2.5-fold higher permeability and cytotoxicity, respectively, in human GBM than stock Bdph. This could be attributed to the high drug loading capacity and the high polarity index due to DMSO, which increases the compatibility between the drug and the cell. Rats bearing a brain glioma treated with 160 mg/kg intranasal emulsified Bdph had a mean survival of 37 days, which is the same survival time achieved by treatment with 320 mg/kg stock Bdph. This implies that the optimized emulsified formulation required only half the Bdph dose to achieve an efficacy similar to that of stock Bdph in the treatment of animals with malignant brain tumor.


Assuntos
Neoplasias Encefálicas/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Emulsões/química , Nanopartículas/química , Mucosa Nasal/fisiologia , Polietilenoglicóis/química , Animais , Neoplasias Encefálicas/patologia , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Glioma/tratamento farmacológico , Glioma/patologia , Humanos , Concentração Inibidora 50 , Masculino , Nanopartículas/ultraestrutura , Tamanho da Partícula , Permeabilidade , Anidridos Ftálicos/química , Ratos Endogâmicos F344 , Solubilidade , Tensoativos/química , Análise de Sobrevida , Carga Tumoral , Viscosidade
12.
Food Chem ; 293: 134-143, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31151593

RESUMO

Thymol oil-in-water nanoemulsions as a potential natural alternative for synthetic antioxidant agents were developed. The nanoemulsions were formulated using Quillaja Saponin bio-surfactant and green solvents including high oleic sunflower oil (HOSO), tricaprylin (TC), and cinnamaldehyde (CA). The 4% thymol nanoemulsions containing TC and HOSO remained stable during long-term storage (at least 30 d). The antioxidant activity (AA) of free thymol and thymol nanoemulsions was compared with butylated hydroxytoluene (BHT) and ascorbic acid. The results obtained from DPPH, FRAP, and CUPRAC antioxidant assays showed a substantial improvement (p < 0.05) of the AA of free thymol through emulsification. The outcomes from the AA of the nanoemulsions in raw chicken breast meat measured by the TBARS assay revealed a significant improvement (p < 0.05) of the AA when thymol was encapsulated. These nanoemulsions may be applicable in the food industry as well as in cosmetic and health care products.


Assuntos
Antioxidantes/química , Emulsões/química , Nanoestruturas/química , Saponinas de Quilaia/química , Tensoativos/química , Timol/química , Animais , Varredura Diferencial de Calorimetria , Galinhas , Carne/análise , Tamanho da Partícula , Quillaja/química , Quillaja/metabolismo , Sonicação
13.
Int J Nanomedicine ; 14: 3955-3966, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31239665

RESUMO

Background: Sulconazole (SCZ) is a broad-spectrum transdermally administered anti-fungicidal agent. However, the therapeutic effect of SCZ is generally limited by its poor water solubility. This present study aimed to develop and evaluate sulconazole-loaded nanoemulsions (SCZ-NEs) for enhancement of the transdermal permeation and antifungal activity. Methods: A spontaneous titration method was applied to prepare the SCZ-NEs. And the optimized formulation of SCZ-NEs was screened by central composite design (CCD). In addition, the characteristics of the SCZ-NEs were evaluated, including particle size, zeta potential, drug loading (DL%) and encapsulation efficiency (EE%). The morphology of SCZ-NEs was observed by transmission electron microscopy (TEM). Franz diffusion cells were used to evaluate the transdermal permeability of the SCZ-NEs. The antifungal activity of the SCZ-NEs was measured by a zone of inhibition (ZOI) test. Results: The optimized SCZ-NEs possessed a moderate particle size of 52.3±3.8 nm, zeta potential of 23.3±1.2 mV, DL% of 0.47±0.05% and EE% of 87.1±3.2%. The ex vivo skin permeation study verified that the cumulative permeability (Qn) and penetration rate (Js) of the optimized SCZ-NEs were about 1.7-fold higher than that of a commercial reference, miconazole (MCZ) cream and 3-fold higher than that of SCZ-DMSO solution. The optimized SCZ-NEs exhibited zone of inhibition (ZOI) values of 23.5±2.4 and 20.4±2.5 mm against C. albicans and T. rubrum, which were larger compared with these of the MCZ cream and SCZ-DMSO solution. Conclusion: SCZ-NEs were effectively developed to overcome the poor solubility of SCZ, promote SCZ permeation through the skin and improve its antifungal activity. Thus, the SCZ-NEs are a promising percutaneous administration for skin fungal infections induced by C. albicans and T. rubrum.


Assuntos
Antifúngicos/farmacologia , Emulsões/química , Imidazóis/farmacologia , Nanopartículas/química , Absorção Cutânea/efeitos dos fármacos , Administração Cutânea , Análise de Variância , Animais , Candida albicans/efeitos dos fármacos , Fungos/efeitos dos fármacos , Masculino , Testes de Sensibilidade Microbiana , Nanopartículas/ultraestrutura , Óleos/química , Tamanho da Partícula , Permeabilidade/efeitos dos fármacos , Transição de Fase , Ratos Sprague-Dawley , Solubilidade , Tensoativos/química
14.
Food Chem ; 295: 180-188, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31174748

RESUMO

Coffee oil is known to be food and therapeutic supplement, both. However, the surface active nature of the oil has not yet been investigated. The present research explored the surface active components in coffee oil, and those responsible for stability of microbubbles at the air-water interface, facilitating its surfactant behavior. The oil's performance was examined through surface tension analysis, foam formation, coalescence rate and foam characteristics (using coffee as a model beverage for foam characterization). These observations confirmed the suitability of coffee oil as a natural substitute for Tween series of surfactants. The 1H NMR and LC-MS/MS assessments revealed trigonelline, caffeic acid, caffeine, feruloyl quinic acid, di-caffeoyl quinic acid, quinic acid, coumaroyl quinic acid as polar constitutes in coffee oil. These constituents help in formation of the self-assembly (chlorogenic acid - hydrophilic head, hydrocarbon - hydrophobic tail), ultimately forming micelle in coffee. Coffee oil also aided maintenance of a well sustained foam in coffee, similar to other synthetic surfactants.


Assuntos
Coffea/química , Café/química , Óleos Vegetais/química , Tensoativos/química , Ácidos Cafeicos/análise , Ácidos Cafeicos/química , Ácido Clorogênico/análise , Cromatografia Líquida , Ácidos Cumáricos/análise , Ácidos Cumáricos/química , Emulsões/química , Interações Hidrofóbicas e Hidrofílicas , Espectroscopia de Ressonância Magnética , Ácido Quínico/análogos & derivados , Ácido Quínico/análise , Ácido Quínico/química , Tensão Superficial , Espectrometria de Massas em Tandem , Viscosidade
15.
AAPS PharmSciTech ; 20(6): 234, 2019 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-31236817

RESUMO

The objective of this study is to explore the surface wettability modulated by a surfactant and its effects on the drug release and absorption of fenofibrate solid dispersions (FF SDs). Both the polyvinylpyrrolidone/sodium lauryl sulfate (PVP/SLS) coprecipitate and FF SDs were prepared by solvent evaporation method. The contact angle of PVP/SLS coprecipitate with various PVP/SLS weight ratios was determined to screen out the suitable content of SLS incorporated in FF SDs. Scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) was used to analyze the surface composition of the PVP/SLS coprecipitate, suggesting that SLS molecules were prone to concentrate on the carrier surface. The physicochemical characteristics of FF, PVP, SLS, FF SDs, and FF physical mixtures (PMs) were evaluated by thermal analysis, XRD, FTIR, and SEM, which revealed that FF was molecularly dispersed in SDs. The interaction between SLS and PVP or FF confirmed by FTIR would affect the surface morphology of SDs. Finally, the contact angle of FF SDs was measured to explore the effects of surface wettability on the dissolution behavior and drug absorption of FF SDs. The interesting thing is that the wettability of the PVP/SLS coprecipitate was positively related to that of FF SDs. The improved wettability of FF SDs or the PVP/SLS coprecipitate by adding SLS contributed to the slight enhancement of initial drug release and absorption, which implied that wettability would be a promising tool in the formulation studies.


Assuntos
Fenofibrato/administração & dosagem , Hipolipemiantes/administração & dosagem , Tensoativos/química , Molhabilidade , Animais , Liberação Controlada de Fármacos , Masculino , Microscopia Eletrônica de Varredura , Povidona/química , Ratos , Ratos Sprague-Dawley , Dodecilsulfato de Sódio/química , Espectrometria por Raios X , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de Superfície
16.
Food Chem ; 293: 368-377, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31151624

RESUMO

In this study, the efficiency and practical utilization feasibility of niosomal and liposomal nanovesicles loading Isoleucine-Proline-Proline (IPP) as suitable ingredients of functional beverages were evaluated. Vesicles were tailored by different preparation methods using phospholipid and non-ionic surfactants. The optimization process was performed by central composite design approach. The results of Fourier transform infrared spectroscopy demonstrated the compatibility of IPP with the vesicles. The phospholipidic nanovesicles, produced by modified ethanol injection-microchannel technique, were smaller with lower polydispersity index than non-ionic surfactant vesicles developed by the method of thin film hydration and probe sonication. However, niosomal model functional beverage exhibited more proper palatability, biological activity and physicochemical properties during long-term storage than liposomal one. Moreover, niosomes exhibited more sustained release behaviour in simulated blood fluid than liposomes. These findings are of great importance for design and development of the functional foods containing IPP.


Assuntos
Bebidas , Alimentos Fortificados , Nanoestruturas/química , Oligopeptídeos/química , Difusão Dinâmica da Luz , Manipulação de Alimentos/métodos , Armazenamento de Alimentos , Suco Gástrico/metabolismo , Humanos , Lipossomos/química , Microscopia Eletrônica de Transmissão , Oligopeptídeos/farmacocinética , Fosfolipídeos/química , Espectroscopia de Infravermelho com Transformada de Fourier , Tensoativos/química
17.
Int J Nanomedicine ; 14: 2973-2983, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31118616

RESUMO

Objective: Intraocular pressure has always been a great challenge for topical ophthalmic drugs. The study aimed to develop ocular surfactant based nanovesicles (NVs) carried in mucoadhesive nanogel providing efficient topical delivery of acetazolamide (ACZ). Methods: For the sake of optimizing formulation parameters, the effect of the type of edge activator and its ratio to sorbitan monostearate (Span 60) on the mean particle size, entrapment efficiency (%EE), and zeta potential (ZP) of produced NVs was investigated. Results: The selected formulation composed of Span 60:sodium deoxycholate with ratio 80:20 showed an average diameter of 202.90 nm, %EE of 90.2%, and ZP of -38.1 mV with a spherical and smooth surface. The ACZ loaded nanovesicles (ACZ-NVs) were embedded in different concentrations of Chitosan-sodium tripolyphosphate (CS-TPP) nanogels. The nanogel prepared using 1.5% CS showed the most promising viscosity, adhesion time, and rheological behavior (118,246 cP, 290 min, and thixotropic behavior, respectively). The in vitro release of ACZ showed a controlled release profile after incorporation in nanogels. The in vivo irritation test showed minimal irritation for the nanogel formulation compared to ACZ topical suspension. The effect of intraocular pressure lowering was significantly prolonged using ACZ-NV nanogels compared to ACZ oral tablets. Histopathological examination emphasized the healing power of CS on retinal atrophy. Conclusion: The research work indicated a promising potential for successful topical delivery of ACZ.


Assuntos
Acetazolamida/administração & dosagem , Acetazolamida/farmacologia , Sistemas de Liberação de Medicamentos , Olho/efeitos dos fármacos , Nanopartículas/química , Polietilenoglicóis/química , Polietilenoimina/química , Tensoativos/química , Animais , Liberação Controlada de Fármacos , Hexoses/química , Pressão Intraocular/efeitos dos fármacos , Masculino , Nanopartículas/administração & dosagem , Nanopartículas/ultraestrutura , Coelhos
18.
Int J Nanomedicine ; 14: 3265-3272, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31118634

RESUMO

Background: In vitro and in vivo studies have shown that metallic implants coated with nano hydroxyapatite (HA) reduce the time needed for complete osseointegration compared to metallic implants coated with conventional or micron-sized HA. Moreover, due to their biologically inspired nanometer dimensions, amphiphilic peptide nanoparticles (APNPs) can also promote osteoblast attachment and enhance other cell functions if used as a coating material. Coatings made of HA and APNPs could improve osteoblast functions, but have never been tested. Purpose: The objective of this study was to prepare coatings of nanocrystalline HA and APNPs on poly(2-hydroxyethyl methacrylate) (pHEMA) coatings in order to improve osteoblast (bone-forming cells) adhesion and cell density. Methods: HA was synthesized by a wet chemical process. Coatings were synthesized with different conditions and components. Results: X-ray diffraction infrared spectroscopy, transmission electron microscopy, and electron diffraction showed that nanocrystalline HA was synthesized with an expected nano size and shape distribution but with low impurities. pHEMA hydrogels with HA and APNPs increased osteoblast densities after 3 days compared to controls. Conclusion: Since cell proliferation is a prerequisite function for bone formation, these results imply that the current materials should be tested for a wide range of orthopedic applications.


Assuntos
Materiais Revestidos Biocompatíveis/química , Durapatita/química , Nanopartículas/química , Osteoblastos/citologia , Peptídeos/química , Tensoativos/química , Contagem de Células , Humanos , Nanopartículas/ultraestrutura , Difração de Raios X
19.
Pharm Res ; 36(7): 107, 2019 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-31111248

RESUMO

PURPOSE: To provide new insights into how protein-surfactant competitive adsorbtion and corresponding surface tension reduction properties at the air-water and oil-water interface are impacted by the type of protein and the associated protein surface rheology. METHOD: Interfacial Rheology was utilized to obtain surface G' and G" as a function of frequency. Force tensiometry was utilized to obtain changes in surface tension as a function of surfactant concentration. The impact on surface properties of two different proteins i.e. BSA and Lysozyme was investigated as a function of surfactant concentration i.e. polysorbates PS 20, PS 80 and Poloxomer (Kolliphor P188). RESULTS: Surface tension and interfacial tension measurements for BSA showed that in mixed BSA/polysorbate surfactant systems, BSA dominates the interfacial behavior at both the air-water and oil-water interfaces, until a high polysorbate concentration of 0.1 mg/ml. At these high polysorbate concentrations a mixed BSA-Polysorbate interfacial layer is formed as corroborated by the surface elasticity values being lower than that of pure BSA but higher than that of pure Polysorbate. For Kolliphor, it was observed that Kolliphor was unable to displace BSA at any concentration. This is corroborated by the high surface elasticity of the BSA which is maintained in the presence of Kolliphor. Surface and interfacial tension measurements for lysozyme show that for mixed lysozyme/polysorbate surfactant systems, the surface tension values are lower than that exhibited by either the lysozyme or the polysorbate surfactants. This potentially indicates the formation of a mixed layer of lysozyme and polysorbate. At the high polysorbate concentrations probed, the surface elasticity values are however closer to that of pure polysorbates, indicating that the mixed layer may be more heavily polysorbate dominated, especially at high polysorbate concentrations. For Kolliphor, the response was similar to that seen in the Kolliphor-BSA system in which the Kolliphor was not able to displace the protein i.e. Lysozyme. CONCLUSIONS: In conclusion, it was seen that competitive adsorption between proteins and common excipient surfactants is dictated by the type of protein and its effective structuring/rigidity at the surface as reflected through surface elasticity and surface tan delta values. BSA was seen to exhibit a higher surface elasticity than lysozyme, and therefore has a more rigid structure and is more competitive at the interface.


Assuntos
Muramidase/química , Poloxâmero/química , Polissorbatos/química , Soroalbumina Bovina/química , Tensoativos/química , Adsorção , Elasticidade , Excipientes , Polietilenoglicóis , Reologia/métodos , Estearatos , Propriedades de Superfície , Tensão Superficial
20.
Chem Commun (Camb) ; 55(49): 6997-7000, 2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31112157

RESUMO

We introduced complementary interactions between peptide amphiphiles and a small fluorescence dye to develop a programmable multi-component supramolecular assembly, and intracellular delivery of the dye was controlled by the dimensions of the co-assembly, which was manipulated by the peptide design.


Assuntos
Sistemas de Liberação de Medicamentos , Corantes Fluorescentes/química , Peptídeos/química , Bibliotecas de Moléculas Pequenas/química , Tensoativos/química , Difusão Dinâmica da Luz , Células HeLa , Humanos , Peptídeos/síntese química , Tensoativos/síntese química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA