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1.
BMC Oral Health ; 21(1): 169, 2021 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-33789646

RESUMO

BACKGROUND: The influence of scaler tip design on root surface roughness, tooth substance loss and patients' pain perception is investigated. METHODS: This article was divided into the following parts: Part 1 Surface roughness and substance loss: an in vitro study, which involves intact extracted teeth sectioned and treated using a piezoelectric ultrasonic device (PM200 EMS Piezon, Switzerland) with a conventional scaler tip (FS-407) and a Perio Slim (PS) scaler tip (Perio Slim DS-016A). All sectioned samples for tooth surface roughness (n = 20) and tooth substance loss (n = 46) analyses were measured and compared using a 3D surface texture analyser and scanning electron microscope (SEM) respectively, at baseline and following scaling. Part 2 Pain Perception: a clinical study, which was a split mouth study design including 30 participants with gingivitis and/or mild chronic periodontitis; treated with supra-gingival scaling from teeth #13 to #23. Subjects were randomised to group A or group B. Group A was treated first with PS scaler tips, whereas group B was treated first with conventional scaler tips. Pain perception was recorded using the visual analogue scale (VAS). RESULTS: In vitro study: both scaler tips caused significant reduction in root substance roughness after scaling (p < 0.05), but no significant difference between the two scaler tips (p > 0.05) was observed. The PS scaler tip caused statistically significantly less root substance loss (p < 0.05) when the initial thickness of the tooth was < 1000 µm. Clinical study: the participants reported significantly lesser pain score during scaling using the PS scaler tip (median: 3) than when using the conventional scaler tip (median: 5) (p < 0.05). CONCLUSIONS: In the in vitro study, using a slim scaler tip design causes less tooth substance loss compared to a wider scaler tip design. In the clinical study, less pain was observed compared than a wide (conventional) scaler tip design.


Assuntos
Raspagem Dentária , Terapia por Ultrassom , Desenho de Equipamento , Humanos , Microscopia Eletrônica de Varredura , Percepção da Dor , Suíça , Raiz Dentária
2.
Int J Nanomedicine ; 16: 2633-2646, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33854312

RESUMO

Background: The specific microenvironment of solid tumors, which is characterized by hypoxia, overexpression of glutathione (GSH), and high accumulation of anti-inflammatory tumor-associated macrophages (TAMs), limits the efficiency of sonodynamic therapy (SDT). Method and Results: Herein, a multifunctional nanoplatform was engineered to modulate the tumor microenvironment for highly efficient SDT. In this system, sonosensitizers and catalase were encapsulated in disulfide-bridged mesoporous organosilicon nanoparticles with high loading, which protected the activity of catalase and ensure the stability of sonosensitizers and enzyme. Subsequently, hyaluronic acid was grafted onto the nanoplatform to reeducate TAMs and induce the secretion of exogenous hydrogen peroxide. Due to the good protection of enzyme, the catalase within the nanoplatform efficiently produced the mount of O2 through decomposing the hydrogen peroxide in tumor tissues, which remarkably alleviated tumor hypoxia. Furthermore, degradation of the nanoparticles was observed in response to GSH, which effectively decreased the intracellular GSH level, further favoring SDT-triggered anticancer effect. Conclusion: Based on the multiple adjustments to tumor microenvironment, our nanoplatform displayed extraordinary sonodynamic therapeutic effect with low systemic toxicity.


Assuntos
Nanopartículas/química , Microambiente Tumoral , Terapia por Ultrassom , Animais , Antineoplásicos/farmacologia , Catalase/metabolismo , Linhagem Celular Tumoral , Polaridade Celular , Liberação Controlada de Fármacos , Feminino , Ácido Hialurônico/química , Peróxido de Hidrogênio/metabolismo , Macrófagos/patologia , Camundongos , Camundongos Endogâmicos BALB C , Nanopartículas/ultraestrutura , Compostos de Organossilício/química , Fenótipo , Porosidade , Porfirinas/farmacologia , Células RAW 264.7 , Hipóxia Tumoral
3.
Rev. Odontol. Araçatuba (Impr.) ; 42(1): 19-23, jan.-abr. 2021. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1148162

RESUMO

O tratamento periodontal consiste na remoção do biofilme patogênico, através da raspagem e alisamento radicular. O desbridamento ultrassônico de boca toda promove uma instrumentação mais conservadora, porém eficiente da superfície radicular, em sessão única. Evitando a translocação bacteriana de uma região tratada para outra que já foi. O objetivo do presente trabalho foi realizar uma comparação entre a eficácia da raspagem manual e a ultrassônica dentro do protocolo da FMD, através de um relato de caso clínico. Houve uma melhora nos parâmetros clínicos periodontais em todos os quadrantes, porém resultados superiores foram observados com o desbridamento com ultrassom e irrigação com clorexidina. A instrumentação com ultrassom associada a clorexidina no tratamento da periodontite estágio III grau C generalizada, reduz com eficácia o tempo de tratamento, otimizando o tempo do paciente e profissional(AU)


Periodontal treatment consists of removing the pathogenic biofilm, by scaling and root planing. Ultrasonic debridement of the entire mouth promotes more conservative, yet efficient instrumentation of the root surface, in a single session. Avoiding bacterial translocation from one treated region to another that has already been. The objective of the present study was to make a comparison between the effectiveness of manual and ultrasonic scraping within the FMD protocol, through a clinical case report. There was an improvement in periodontal clinical parameters in all quadrants, but superior results were observed with debridement with ultrasound and irrigation with chlorhexidine. Instrumentation with ultrasound associated with chlorhexidine in the treatment of generalized stage III grade C periodontitis, effectively reduces treatment time, optimizing patient and professional time(AU)


Assuntos
Periodontite , Raspagem Dentária , Desbridamento Periodontal , Terapia por Ultrassom , Clorexidina , Placa Dentária
4.
Medicine (Baltimore) ; 100(10): e24764, 2021 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-33725832

RESUMO

BACKGROUND: To our knowledge, only 1 study with limited sample size tried to evaluate the synergistic effects of ultrasound and low-level laser therapy (LLLT) in patients with knee osteoarthritis. Further research is needed to confirm this synergy with larger numbers and better design. Therefore, we will conduct this present randomized double-blind study to evaluate the synergistic effects of simultaneously applying ultrasound plus LLLT on pain and muscle function in patients with knee osteoarthritis. METHODS: The study protocol is a randomized, controlled, double-blind design. The study will be conducted at our academic hospital from February 2021 to January 2022. The study protocol was approved through Institutional Review Board in the Hunan Provincial People's Hospital. Patients will be assigned at random to the ultrasound + LLLT group, LLLT group, or the ultrasound group. After baseline examination, all patients will be given a full explanation of the treatment protocol and will be required to sign a written informed consent for study participation and for publication of the results. All the data collectors, surgeons, statistical analysts, as well as result assessors are not aware of grouping assignment. The primary outcome is weekly change in pain intensity relative to baseline through 6 weeks of therapy. RESULTS: This protocol will provide a reliable theoretical basis for the following research. CONCLUSION: It is assumed that there will be a remarkable difference in postoperative outcomes between the intervention and control groups. TRIAL REGISTRATION: This study protocol was registered in Research Registry (researchregistry6470).


Assuntos
Terapia com Luz de Baixa Intensidade , Músculo Esquelético/fisiopatologia , Osteoartrite do Joelho/fisiopatologia , Osteoartrite do Joelho/terapia , Manejo da Dor , Terapia por Ultrassom , Terapia Combinada , Método Duplo-Cego , Humanos
5.
Int J Nanomedicine ; 16: 1889-1899, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33707944

RESUMO

Introduction: Sonodynamic therapy (SDT) has good targeting and non-invasive advantages in the treatment of solid cancers, and checkpoint blockade immunotherapy is also a promising treatment to cure cancer. However, their antitumor effects are not sufficient due to some inherent factors. Some studies that combined SDT with immunotherapy or nanoparticles have managed to enhance its efficiency to treat cancers. Methods: In this work, an effective therapeutic strategy that can potentiate the antitumor efficacy of anti-PD-L1 antibody (aPD-L1) is developed by the use of cascade immuno-sonodynamic therapy (immuno-SDT). Titanium dioxide (TiO2), a nanostructured agent for SDT, sonosensitizer Chlorin e6 (Ce6), and immunological adjuvant CpG oligonucleotide (CpG ODN), are used to construct a multifunctional nanosonosensitizer (TiO2-Ce6-CpG). Then, we conducted in vitro and in vivo experiments to explore the antitumor effect of TiO2-Ce6-CpG under ultrasound (US) treatment. Results: The characterization tests showed that the nanosonosensitizers are polycrystalline structure with homogeneous sizes, resulting in a good drug loading efficiency. The innovative nanosonosensitizers (TiO2-Ce6-CpG) can not only effectively inhibit tumor growth but also stimulate the immune system to activate the adaptive immune responses, using the TiO2-Ce6 to augment SDT and the immune adjuvant CpG to enhance the immune response. After combined with the aPD-L1, the synergistic effect could not only efficiently inhibit the primary tumor growth but also lead to an inhibition of the non-irradiated pre-existing distant tumors by inducing a strong tumor-specific immune response. Conclusion: In this study, we present an effective strategy for tumor treatment by combining nanosonosensitizer-augmented SDT and aPD-L1 checkpoint blockade. This work provides a promising strategy and offers a new vision for treating malignant tumors.


Assuntos
/uso terapêutico , Imunoterapia , Nanopartículas/química , Neoplasias/imunologia , Neoplasias/terapia , Terapia por Ultrassom , Animais , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Terapia Combinada , Células Dendríticas/efeitos dos fármacos , Endocitose/efeitos dos fármacos , Feminino , Humanos , Imunidade/efeitos dos fármacos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Nanopartículas/ultraestrutura , Neoplasias/patologia , Oligodesoxirribonucleotídeos/química , Porfirinas/química , Espécies Reativas de Oxigênio/metabolismo , Titânio/química
6.
Int J Nanomedicine ; 16: 1913-1926, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33707946

RESUMO

Purpose: Immunologically quiescent of breast cancer cells has been recognized as the key impediment for the breast cancer immunotherapy. In this study, we aimed to investigate the role of nanoparticle-mediated sonodynamic therapy (SDT) in promoting anti-tumor immune of breast cancer cells and its potential immune mechanisms. Materials and Methods: The phase-transformation nanoparticles (LIP-PFH nanoparticles) were in-house prepared and its physiochemical characters were detected. The CCK-8 assay, apoptosis analysis and Balb/c tumor model establishment were used to explore the anti-tumor effect of LIP-PFH nanoparticles triggered by low-intensity focused ultrasound (LIFU) both in vitro and in vivo. Flow cytometry and immunohistochemistry of CD4+T, CD8+T, CD8+PD-1+T in blood, spleen and tumor tissue were performed to represent the change of immune response. Detection of immunogenic cell death (ICD) markers was examined to study the potential mechanisms. Results: LIP-PFH nanoparticles triggered by LIFU could inhibit the proliferation and promote the apoptosis of 4T1 cells both in vitro and in vivo. CD4+T and CD8+T cell subsets were significantly increased in blood, spleen and tumor tissue, meanwhile CD8+PD-1+T cells were reduced, indicating enhancement of anti-tumor immune response of breast cancer cells in the nanoparticle-mediated SDT group. Detection of ICD markers (ATP, high-mobility group box B1, and calreticulin) and flow cytometric analysis of dendritic cell (DC) maturity further showed that the nanoparticle-mediated SDT can promote DC maturation to increase the proportion of cytotoxic T cells by inducing ICD of breast cancer cells. Conclusion: The therapy of nanoparticles-mediated SDT can effectively enhance anti-tumor immune response of breast cancer.


Assuntos
Imunidade , Morte Celular Imunogênica , Neoplasias Mamárias Animais/imunologia , Neoplasias Mamárias Animais/terapia , Nanopartículas/química , Terapia por Ultrassom , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Células Dendríticas/metabolismo , Endocitose , Feminino , Fluorcarbonetos/química , Imunoterapia , Neoplasias Mamárias Animais/patologia , Camundongos , Camundongos Endogâmicos BALB C , Nanopartículas/ultraestrutura , Linfócitos T/imunologia , Linfócitos T Citotóxicos/imunologia
7.
J R Soc Interface ; 18(176): 20200967, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33757291

RESUMO

As a result of the outbreak and diffusion of SARS-CoV-2, there has been a directive to advance medical working conditions. In dentistry, airborne particles are produced through aerosolization facilitated by dental instruments. To develop methods for reducing the risks of infection in a confined environment, understanding the nature and dynamics of these droplets is imperative and timely. This study provides the first evidence of aerosol droplet formation from an ultrasonic scalar under simulated oral conditions. State-of-the-art optical flow tracking velocimetry and shadowgraphy measurements are employed to quantitatively measure the flow velocity, trajectories and size distribution of droplets produced during a dental scaling process. The droplet sizes are found to vary from 5 µm to 300 µm; these correspond to droplet nuclei that could carry viruses. The droplet velocities also vary between 1.3 m s-1 and 2.6 m s-1. These observations confirm the critical role of aerosols in the transmission of disease during dental procedures, and provide invaluable knowledge for developing protocols and procedures to ensure the safety of both dentists and patients.


Assuntos
Aerossóis , Microbiologia do Ar , Instrumentos Odontológicos , Terapia por Ultrassom , /prevenção & controle , Humanos
8.
Biomater Sci ; 9(6): 1945-1960, 2021 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-33522523

RESUMO

As an alternative to photodynamic therapy (PDT), ultrasound-triggered tumor sonodynamic therapy (SDT) has garnered significant attention, owing to its high tissue penetration, few side effects, and reliable patient compliance. A sonosensitizer is the most important component in SDT, and high-quantum-yield safe sonosensitizers are crucial for SDT. Existing sonosensitizers mainly include organic sonosensitizers and inorganic sonosensitizers. Organic sonosensitizers, mainly some small dye molecules, have been widely studied. However, organic sonosensitizers have limited utility owing to their poor stability, rapid blood clearance, and potential phototoxicity. In contrast, inorganic sonosensitizers have stable chemical properties, long circulation time in the blood and can effectively reduce phototoxicity. In addition to their utilization as sonosensitizers, some inorganic nanoparticles can also operate as carriers for delivering organic sonosensitizers, effectively overcoming the inherent shortcomings of organic small-molecule sonosensitizers. This review mainly focuses on inorganic nanomaterial-based SDT, the possible mechanisms of SDT, and newly developed inorganic sonosensitizers, as well as the challenges and possible solutions associated with their clinical translation are introduced.


Assuntos
Antineoplásicos , Nanopartículas , Neoplasias , Terapia por Ultrassom , Antineoplásicos/uso terapêutico , Terapia Combinada , Humanos , Neoplasias/tratamento farmacológico
9.
Nanoscale ; 13(8): 4512-4518, 2021 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-33615325

RESUMO

In combating cancer, ultrasound (US)-triggered sonodynamic therapy (SDT) manifests a wide range of promising applications as a noninvasive treatment modality, thus showing potential to overcome the shortcomings and disadvantages of conventional photodynamic therapy (PDT). Reactive oxygen species (ROS)-based therapy is practically destroyed by the high concentration of glutathione (GSH) inside tumors, and depleting GSH to improve the outcome of SDT is indeed a great challenge. Herein, we designed GSH-depleting nanoplatelets for enhanced sonodynamic cancer therapy. A platelet membrane coated nanosystem (PSCI) has been designed and tested comprising mesoporous silica nanoparticles (MSNs) which have been loaded with cinnamaldehyde (CA) as an oxidative stress amplifier. The inner layer comprises the sonosensitizer IR780 and the oxidative stress amplifier CA, whereas the platelet membranes (PM) were designed and utilized as an outer layer that can target tumors, thereby enhancing the effectiveness of SDT by attenuating the capability of tumor cells for scavenging ROS with GSH. SDT and cinnamaldehyde amplify oxidative stress by acting synergistically, leading to the preferential destruction of cancer cells in vitro and in vivo. It is hoped that next-generation tumor SDT treatments will find their way with the help of this strategy.


Assuntos
Nanopartículas , Neoplasias , Terapia por Ultrassom , Linhagem Celular Tumoral , Glutationa , Neoplasias/tratamento farmacológico , Espécies Reativas de Oxigênio
10.
Cochrane Database Syst Rev ; 1: CD012479, 2021 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-33434949

RESUMO

BACKGROUND: Various rehabilitation treatments may be offered following surgery for flexor tendon injuries of the hand. Rehabilitation often includes a combination of an exercise regimen and an orthosis, plus other rehabilitation treatments, usually delivered together. The effectiveness of these interventions remains unclear. OBJECTIVES: To assess the effects (benefits and harms) of different rehabilitation interventions after surgery for flexor tendon injuries of the hand. SEARCH METHODS: We searched the Cochrane Central Register of Controlled Trials, the Cochrane Bone, Joint and Muscle Trauma Group Specialised Register, MEDLINE, Embase, two additional databases and two international trials registries, unrestricted by language. The last date of searches was 11 August 2020. We checked the reference lists of included studies and relevant systematic reviews. SELECTION CRITERIA: We included randomised controlled trials (RCTs) and quasi-RCTs that compared any postoperative rehabilitation intervention with no intervention, control, placebo, or another postoperative rehabilitation intervention in individuals who have had surgery for flexor tendon injuries of the hand. Trials comparing different mobilisation regimens either with another mobilisation regimen or with a control were the main comparisons of interest. Our main outcomes of interest were patient-reported function, active range of motion of the fingers, and number of participants experiencing an adverse event. DATA COLLECTION AND ANALYSIS: Two review authors independently selected trials for inclusion, extracted data, assessed risk of bias and assessed the quality of the body of evidence for primary outcomes using the GRADE approach, according to standard Cochrane methodology. MAIN RESULTS: We included 16 RCTs and one quasi-RCT, with a total of 1108 participants, mainly adults. Overall, the participants were aged between 7 and 72 years, and 74% were male. Studies mainly focused on flexor tendon injuries in zone II. The 17 studies were heterogeneous with respect to the types of rehabilitation treatments provided, intensity, duration of treatment and the treatment setting. Each trial tested one of 14 comparisons, eight of which were of different exercise regimens. The other trials examined the timing of return to unrestricted functional activities after surgery (one study); the use of external devices applied to the participant to facilitate mobilisation, such as an exoskeleton (one study) or continuous passive motion device (one study); modalities such as laser therapy (two studies) or ultrasound therapy (one study); and a motor imagery treatment (one study). No trials tested different types of orthoses; different orthosis wearing regimens, including duration; different timings for commencing mobilisation; different types of scar management; or different timings for commencing strengthening. Trials were generally at high risk of bias for one or more domains, including lack of blinding, incomplete outcome data and selective outcome reporting. Data pooling was limited to tendon rupture data in a three trial comparison. We rated the evidence available for all reported outcomes of all comparisons as very low-certainty evidence, which means that we have very little confidence in the estimates of effect. We present the findings from three exercise regimen comparisons, as these are commonly used in clinical current practice. Early active flexion plus controlled passive exercise regimen versus early controlled passive exercise regimen (modified Kleinert protocol) was compared in one trial of 53 participants with mainly zone II flexor tendon repairs. There is very low-certainty evidence of no clinically important difference between the two groups in patient-rated function or active finger range of motion at 6 or 12 months follow-up. There is very low-certainty evidence of little between-group difference in adverse events: there were 15 overall. All three tendon ruptures underwent secondary surgery. An active exercise regimen versus an immobilisation regimen for three weeks was compared in one trial reporting data for 84 participants with zone II flexor tendon repairs. The trial did not report on self-rated function, on range of movement during three to six months or numbers of participants experiencing adverse events. The very low-certainty evidence for poor (under one-quarter that of normal) range of finger movement at one to three years follow-up means we are uncertain of the finding of zero cases in the active group versus seven cases in the immobilisation regimen. The same uncertainty applies to the finding of little difference between the two groups in adverse events (5 tendon ruptures in the active group versus 10 probable scar adhesion in the immobilisation group) indicated for surgery. Place and hold exercise regimen performed within an orthosis versus a controlled passive regimen using rubber band traction was compared in three heterogeneous trials, which reported data for a maximum of 194 participants, with mainly zone II flexor tendon repairs. The trials did not report on range of movement during three to six months, or numbers of participants experiencing adverse events. There was very low-certainty evidence of no difference in self-rated function using the Disability of the Arm, Shoulder and Hand (DASH) functional assessment between the two groups at six months (one trial) or at 12 months (one trial). There is very low-certainty evidence from one trial of greater active finger range of motion at 12 months after place and hold. Secondary surgery data were not available; however, all seven recorded tendon ruptures would have required surgery. All the evidence for the other five exercise comparisons as well as those of the other six comparisons made by the included studies was incomplete and, where available, of very low-certainty. AUTHORS' CONCLUSIONS: There is a lack of evidence from RCTs on most of the rehabilitation interventions used following surgery for flexor tendon injuries of the hand. The limited and very low-certainty evidence for all 14 comparisons examined in the 17 included studies means that we have very little confidence in the estimates of effect for all outcomes for which data were available for these comparisons. The dearth of evidence identified in this review points to the urgent need for sufficiently powered RCTs that examine key questions relating to the rehabilitation of these injuries. A consensus approach identifying these and establishing minimum study conduct and reporting criteria will be valuable. Our suggestions for future research are detailed in the review.


Assuntos
Traumatismos da Mão/reabilitação , Traumatismos dos Tendões/reabilitação , Adolescente , Adulto , Idoso , Viés , Criança , Terapia por Exercício/efeitos adversos , Terapia por Exercício/métodos , Exoesqueleto Energizado , Feminino , Traumatismos da Mão/cirurgia , Humanos , Imobilização , Terapia a Laser , Masculino , Pessoa de Meia-Idade , Contração Muscular/fisiologia , Cuidados Pós-Operatórios/métodos , Ensaios Clínicos Controlados Aleatórios como Assunto/estatística & dados numéricos , Amplitude de Movimento Articular , Ruptura/reabilitação , Ruptura/cirurgia , Traumatismos dos Tendões/cirurgia , Terapia por Ultrassom , Adulto Jovem
11.
ACS Appl Mater Interfaces ; 13(5): 6099-6108, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33507729

RESUMO

The blood-brain barrier (BBB) is a physical barrier that selectively prevents certain substances from entering the brain through the blood. The BBB protects the brain from germs and causes difficulty in intracranial treatment. The chemotherapy drug temozolomide (TMZ), embedded in nanobubbles (NBs) and combined with persistent luminescent nanoparticles (PLNs), has been used to treat glioblastoma (GBM) effectively through image tracking. Through ultrasound induction, NBs produce cavitation that temporarily opens the BBB. Additionally, the PLNs release near-infrared emission and afterglow, which can penetrate deep tissues and improve the signal-to-noise ratio of bioimages. In this work, the nanosystem crossed the BBB for drug delivery and image tracking over time, allowing the enhancement of the drug's therapeutic effect on GBM. We hope that this nanosystem can be applied to the treatment of different brain diseases by embedding different drugs in NBs.


Assuntos
Antineoplásicos Alquilantes/farmacologia , Neoplasias Encefálicas/terapia , Glioblastoma/terapia , Nanocompostos/química , Temozolomida/farmacologia , Terapia por Ultrassom , Animais , Antineoplásicos Alquilantes/química , Barreira Hematoencefálica/efeitos dos fármacos , Neoplasias Encefálicas/patologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Glioblastoma/patologia , Humanos , Raios Infravermelhos , Estrutura Molecular , Neoplasias Experimentais/patologia , Neoplasias Experimentais/terapia , Tamanho da Partícula , Propriedades de Superfície , Temozolomida/química , Ondas Ultrassônicas
12.
ACS Appl Mater Interfaces ; 13(4): 4825-4834, 2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33496168

RESUMO

Ultrasound (US)-induced sonodynamic therapy (SDT) is an efficient and precise method against tumor, and the integration of multiple cancer therapies has been proved as a promising strategy for better therapeutic effects. Herein, for the first time, a multifunctional nanoreactor has been fabricated by integrating Fe-MIL-88B-NH2, PFC-1, and glucose oxidase (GOx) to form urchin-like Fe-MIL-88B-NH2@PFC-1-GOx (MPG) nanoparticles as Fenton's reagent, a sonosensitizer, and a tumor microenvironment (TME) modulator. In detail, MPG can generate •OH for chemodynamic therapy (CDT) and deplete glutathione (GSH) to alleviate the antioxidant ability of cancer cells. Moreover, catalase (CAT)-like MPG can react with H2O2 to generate O2 for relieving hypoxia in TME, enhancing GOx-catalyzed glucose oxidation to produce H2O2 and gluconic acid. Then, the regenerated H2O2 can promote the Fenton reaction to achieve GOx catalysis-enhanced CDT. Owing to its large π-electron conjugated system, MPG also serves as an ideal sonosensitizer, realizing a burst generation of 1O2 under US irradiation for efficient SDT. Therefore, the tumor treatment will be notably enhanced by MPG-based synergetic CDT/SDT/starvation therapy via a series of cascade reactions. Overall, this work develops a versatile nanoreactor with improved tumor treatment effectiveness and broadens the application prospects of porous materials in the field of biomedical research.


Assuntos
Glucose Oxidase/uso terapêutico , Neoplasias Mamárias Animais/terapia , Estruturas Metalorgânicas/uso terapêutico , Nanocompostos/uso terapêutico , Animais , Biocatálise , Catálise , Linhagem Celular Tumoral , Feminino , Glucose Oxidase/química , Glutationa/metabolismo , Ligação de Hidrogênio , Peróxido de Hidrogênio/metabolismo , Neoplasias Mamárias Animais/metabolismo , Neoplasias Mamárias Animais/patologia , Estruturas Metalorgânicas/química , Camundongos , Camundongos Endogâmicos BALB C , Modelos Moleculares , Nanocompostos/química , Nanocompostos/ultraestrutura , Nanomedicina , Hipóxia Tumoral/efeitos dos fármacos , Terapia por Ultrassom
13.
Bauru; s.n; 2021. 23 p. tab.
Tese em Português | Sec. Est. Saúde SP, CONASS, HANSEN, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP, SESSP-ESPECIALIZACAOSESPROD, Sec. Est. Saúde SP | ID: biblio-1179317

RESUMO

A Hanseníase é uma doença crônica, de evolução lenta e infectocontagiosa. Causada pelo bacilo intracelular Mycobacterium Leprae, afeta pele e nervos periféricos, possui grande potencial de gerar lesões irreversíveis, e representa um importante problema de saúde pública por ainda permanecer endêmica. Mesmo após a cura, o bacilo pode deixar antígenos persistentes, os quais estimulam processos inflamatórios recorrentes, e esses podem levar a dor neuropática, sendo esta classificada como a que mais deteriora profundamente a qualidade de vida de seus portadores, haja vista a sua tendência a cronicidade e resistência às terapias analgésicas. A fisioterapia contempla inúmeras intervenções que são empregadas para o alívio da dor sendo o ultrassom terapêutico um método com potencial efeito analgésico. Baseado nisso, esta revisão de literatura teve como objetivo avaliar a eficácia e eficiência do ultrassom terapêutico sobre a percepção da dor neuropática em pacientes portadores de hanseníase. Foram realizadas pesquisas até fevereiro de 2021 nas seguintes bases de dados eletrônicas: Pubmed, Pedro, Cochrane, Embase e BVS MS, incluindo todos os modelos de artigos científicos (observacional, descritivo ou experimental), informações de livros, dissertações, monografias, teses e listas de referências resultantes das buscas. Foram encontrados apenas dois artigos e um capitulo de livro que abrangesse o tema proposto. Concluímos que evidências de baixa certeza mostram que o ultrassom terapêutico pode ser eficaz, no entanto, devido ao limitado número de estudos inseridos, necessitam-se novas pesquisas para comprovar sua efetividade e eficácia como método de alivio da dor neuropatia em pacientes com hanseníase.


Assuntos
Terapia por Ultrassom , Doenças do Sistema Nervoso Periférico/reabilitação , Hanseníase/terapia , Analgesia , Hanseníase/reabilitação
14.
Biomaterials ; 264: 120386, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32979656

RESUMO

The precise treatment of drug-resistant deep bacterial infections remains a huge challenge in clinic. Herein, a polymer-peptide-porphyrin conjugate (PPPC), which can be real-time monitored in infectious site, is developed for accurate and deep sonodynamic therapy (SDT) based on "in vivo self-assembly" strategy. The PPPC contains four moieties, i.e., a hyperbranched polymer backbone, a self-assembled peptide linked with an enzyme-cleavable peptide-poly (ethylene glycol) terminal, a bacterial targeting peptide, and a porphyrin sonosensitizer (MnTCPP) segment. Once PPPC nanoparticles reach the infectious area, the protecting PEG layers are removed due to the over-expressed gelatinase, leading to the secondary assembly into large nanoaggregates and resultant enhanced accumulation of sonosensitizer. The nanoaggregates exhibit enhanced interaction with bacterial membrane and decrease the minimum inhibitory concentration (MIC) significantly. Meanwhile, compared with free MnTCPP, the concentration of which can not be accurately quantified, the accumulation amount of MnTCPP in PPPCs at infectious site can be in situ monitored by magnetic resonance imaging (MRI) using T1 combined with T2. When the concentration of PPPC-1 reaches MIC, the drug-resistant bacterial infection area is exposed to ultrasound irradiation, causing the precise and efficient elimination of bacteria. Therefore, the MRI-guided SDT system shows extraordinary tissue penetration depth, drug concentration monitoring, morphology-transformation induced accumulation and improved treatment capacity toward drug-resistant bacteria.


Assuntos
Nanopartículas , Preparações Farmacêuticas , Terapia por Ultrassom , Bactérias , Imagem por Ressonância Magnética
15.
Cancer Lett ; 497: 229-242, 2021 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-33122099

RESUMO

Sonodynamic therapy (SDT) represents a promising modality that provides the possibility of non-invasively eliminating solid tumors in a site-directed manner. In light of the complexity and heterogeneity of tumors, more and more studies are attempting to combine SDT with other therapeutic methods so as to achieve better tumor treatment effect, which sheds new light on the potential of SDT-based synergistic therapeutics. Herein, the representative studies of SDT-instructed multimodal synergistic cancer therapy are comprehensively presented, such as sono-chemotherapy, sono-radiotherapy, sono-immunotherapy, and sono-chemodynamic therapy, etc., and their incorporate mechanisms are discussed in detail. The current challenges and future prospects to promote the advanced development of SDT-based nanomedicines in this burgeoning research field are highlighted. It is believed that such an emerging synergistic therapeutic modality based on SDT will play a more significant role in the field of tumor precision treatment medicine.


Assuntos
Imunoterapia , Nanomedicina , Neoplasias/terapia , Terapia por Ultrassom , Animais , Terapia Combinada , Humanos , Neoplasias/imunologia , Neoplasias/patologia
16.
Ultrasonics ; 110: 106272, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33065465

RESUMO

Sonodynamic therapy (SDT) is a promising modality for cancer treatment. Sinoporphyrin sodium (DVDMS), purified from Photofrin II, shows great potential in SDT evidenced by growing studies. The purpose of the current study was to investigate the antitumor effect of SDT combined with DVDMS on human glioblastoma (U87 MG) cell line in vitro. The cellular uptake of DVDMS was investigated by confocal microscopy and IVIS spectrum imaging system. In addition, DVDMS toxicity and anti-tumor effect of SDT were assessed by flow cytometry. The generation of intracellular reactive oxygen species (ROS) was determined using DCFH-DA staining. Simultaneously, fluorescence microscopy was performed to access the destabilization of mitochondrial membrane potential (MMP). The results showed that DVDMS could easily enter the cells and accumulated in the cytoplasm, especially the mitochondria. And the intracellular DVDMS increased with incubation time or concentrations. The results also showed remarkable cytotoxicity of DVDMS-mediated SDT (center frequency: 0.970 MHz; peak-rarefactional pressure: 0.52-MPa; acoustic power: 0.32 W; pulse repetition frequency: 1 Hz; duty cycle: 1-30%; duration: 3 min) on U87 MG cells, while DVDMS alone was non-toxic to the cells. In comparison with the control group, the SDT-treated group showed significant generation of intracellular ROS and loss of MMP at 1 h post-treatment. These results indicated that DVDMS-mediated SDT could induce great cytotoxicity in U87 MG cells via the production of ROS and showed potentials in the treatment for glioblastoma.


Assuntos
Antineoplásicos/farmacologia , Glioblastoma/terapia , Porfirinas/farmacologia , Terapia por Ultrassom/métodos , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Desenho de Equipamento , Humanos , Técnicas In Vitro , Potencial da Membrana Mitocondrial , Necrose , Espécies Reativas de Oxigênio/metabolismo
17.
Estima (Online) ; 18(1): e3320, jan.-dez. 2020.
Artigo em Português | BDENF - Enfermagem | ID: biblio-1148212

RESUMO

Objetivo: avaliar a eficácia do ultrassom de baixa frequência associado ao gel de óleos de copaíba e melaleuca na cicatrização de lesões de pele. Método: estudo quase-experimental com pré e pós-testes em grupo único, realizado em hospital público no Ceará, Brasil, no período de março a setembro de 2017, com 14 participantes que receberam 10 sessões de tratamento. Resultados: houve redução média de mais de 20 cm2 da 1ª para 5ª aplicação. A redução no tamanho inicial para a 10ª sessão foi superior a 50%. Houve significância estatística (p=0,0043) entre a avaliação inicial e a 10ª avaliação da terapia, com redução média de 37 cm2. Houve redução na intensidade da dor relatada pelos participantes e 62,5% estavam livres da dor após aplicação da terapia. Conclusão: o uso do gel com óleo de copaíba e melaleuca aponta para uma alternativa de cobertura associada a bioestimuladores como o ultrassom. A composição do óleo em gel auxiliou na aplicação e na redução de maceração do bordo das feridas bem como o controle do exsudato da lesão.


Assuntos
Terapia por Ultrassom , Cicatrização , Óleos Voláteis
18.
Transl Vis Sci Technol ; 9(13): 16, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33344060

RESUMO

Purpose: Corneal neovascularization (CNV) is the invasion of new blood vessels into the avascular cornea, leading to reduced corneal transparency and visual acuity, impaired vision, and even blindness. Current treatment options for CNV are limited. We developed a novel treatment method, termed photo-mediated ultrasound therapy (PUT), that combines laser and ultrasound, and we tested its feasibility for treating CNV in a rabbit model. Methods: A suture-induced CNV model was established in New Zealand White rabbits, which were randomly divided into two groups: PUT and control. For the PUT group, the applied light fluence at the corneal surface was estimated to be 27 mJ/cm2 at 1064-nm wavelength with a pulse duration of 5 ns, and the ultrasound pressure applied on the cornea was 0.43 MPa at 0.5 MHz. The control group received no treatment. Red-free photography and fluorescein angiography were utilized to evaluate the efficiency of PUT. Safety was evaluated by histology and immunohistochemistry. For comparison with the PUT safety results, conventional laser photocoagulation (LP) treatment was performed with standard clinical parameters: 532-nm continuous-wave (CW) laser with 0.1-second pulse duration, 450-mW power, and 75-µm spot size. Results: In the PUT group, only 1.8% ± 0.8% of the CNV remained 30 days after treatment. In contrast, 71.4% ± 7.2% of the CNV remained in the control group after 30 days. Safety evaluations showed that PUT did not cause any damage to the surrounding tissue. Conclusions: These results demonstrate that PUT is capable of removing CNV safely and effectively in this rabbit model. Translational Relevance: PUT can remove CNV safely and effectively.


Assuntos
Neovascularização da Córnea , Terapia por Ultrassom , Animais , Neovascularização da Córnea/terapia , Angiofluoresceinografia , Lasers , Coelhos , Acuidade Visual
19.
Endodoncia (Madr.) ; 38(3): 6-15, dic. 2020. ilus, tab, graf
Artigo em Espanhol | IBECS | ID: ibc-200308

RESUMO

OBJETIVO: Comparar, en un estudio experimental in vitro, el porcentaje de áreas no obturadas entre la técnica de compactación lateral en frío (TCL) y la técnica de compactación lateral con ultrasonido (TCLU). MATERIALES Y MÉTODOS: El estudio fue realizado en una muestra de 68 dientes humanos extraídos. Se asignaron de manera aleatoria en dos grupos de 34 dientes cada uno. Todos los dientes se cortaron a 16±1 mm de longitud desde el ápice hacia la corona, se instrumentaron con el sistema rotatorio Protaper Next (Dentsply) y se realizó protocolo de irrigación final con NaOCl al 2.5% y EDTA al 17% neutralizados con suero fisiológico entre ellos. El Grupo I se obturó con TCL y el Grupo II con TCLU. En ambos grupos se utilizó Sealapex como cemento. Los dientes fueron seccionados horizontalmente a 2, 4, 6 y 8 mm desde el ápice; cada corte se observó con un microscopio estereoscópico (56X) y se tomaron fotografías. Se calculó el porcentaje de área obturada con gutapercha, cemento y de áreas no obturadas, utilizando el programa ImageJ. El análisis de los resultados se llevó acabo mediante la suma de rangos de Wilcoxon. RESULTADOS: El grupo TCLU presentó un mayor porcentaje de gutapercha en el conducto radicular en comparación con el grupo TCL (94.4% y 87.4% respectivamente), menor porcentaje de áreas no obturadas (1.3% y 4.2%), y menor porcentaje de áreas ocupadas con cemento (3.8% y 7.2%). Las diferencias fueron estadísticamente significativas (P<0.05).CONCLUSIÓN: Los resultados de este estudio muestran que la modificación de la técnica de compactación lateral con ultrasonido descrita en esta investigación presenta resultados in vitro superiores a TCL


AIM: To compare, in an Experimental in vitro study, the percentage of non-filled areas between cold lateral compaction technique (CLT) and lateral compaction with ultrasonic technique (LUT). METHODS: The study was made on in a sample of 68 extracted human teeth. They were randomized into two groups of 34 teeth each. All teeth were cut 16±1 mm from the apex and instrumented with the Protaper Next rotary system (Dentsply) and a final irrigation protocol was performed with NaOCl 2.5% and EDTA 17% neutralized with normal saline solution between them. Group I was filled with CLT and Group II with LUT. In both groups Sealapex was used as a cement. The teeth were sectioned horizontally at 2, 4, 6 and 8 mm from the apex; slices were the viewed through a stereomicroscope (56X) and pictures were taken. The percentage of gutta-percha filled area, percentage of non-filled area and percentage of sealer filled area were calculated for each section using the ImageJ program. Statistical analysis was performed using the Wilcoxon Rank-Sum test. RESULTS: LUT had a higher percentage of gutta-percha in the root canal compared to CLT (94.4% and 87.4% respectively), lower percentage of non-filled area (1.3% and 4.2% respectively), and lower percentage of area occupied by sealer (3.8 % and 7.2% respectively). The differences were statistically significant(P <0.05).CONCLUSION: The results of this study showed that the modification of lateral compaction technique with ultrasonic described in this investigation presents in vitro results superior to CLT


Assuntos
Humanos , Obturação do Canal Radicular/métodos , Terapia por Ultrassom/métodos , Guta-Percha/uso terapêutico , Cimentos Dentários/uso terapêutico , Teste de Materiais , Reprodutibilidade dos Testes , Hidróxido de Cálcio/uso terapêutico , Salicilatos/uso terapêutico
20.
J Cancer Res Ther ; 16(6): 1250-1257, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33342780

RESUMO

Purpose: We want to compare the synergistic effect of low-intensity, dual-frequency (dual) ultrasound (US), applied in a repetitive sonication mode, and acoustically active lipospheres (AALs) containing doxorubicin (DOX) in a murine model (Balb/C). Subjects and Methods: The tumor-bearing mice were divided into nine groups, namely two untreated groups (control and sham), and seven experimental groups, including treated with dual-frequency US (150 kHzcontinuous + 1MHzpulse), triple exposure (3×30min) dual-frequency US, DOX (2 mg/kg intravenous), DOX in combination with single exposure (30 min) to dual-frequency US (drug + dual), DOX in combination with triple (3 × 30 min) exposure to dual-frequency US (drug + dual [REP]), AALs containing the drug-loaded (AAL), and a group receiving AAL in combination with single exposure (30 min) dual-frequency US (AAL + dual), respectively. Results: The effectiveness of DOX on tumor growth was enhanced by a factor of three when combined with the triple exposures of dual US (drug + dual [REP]). This combination protocol further increased the times needed for each tumor to 2 and 7 times its initial volume, respectively by 94% and 36% compared to the drug group. During the 30 days, following the treatment of tumors, the relative volume of tumors in AAL group was 118% less than that of the drug group. The survival rate of the groups treated with drug and AAL + dual was increased by 78.7% and 167% compared with sham, respectively. Conclusion: Although as a short treatment, a major improvement in treatment was observed by (drug + dual [REP]) compared with other treatments, the AAL + dual treatment compared with (drug + dual [REP]) showed an increase in the survival rates, hence more preferable over long periods.


Assuntos
Adenocarcinoma/terapia , Neoplasias da Mama/terapia , Doxorrubicina/administração & dosagem , Portadores de Fármacos/química , Terapia por Ultrassom/métodos , Adenocarcinoma/tratamento farmacológico , Animais , Neoplasias da Mama/tratamento farmacológico , Linhagem Celular Tumoral , Modelos Animais de Doenças , Doxorrubicina/química , Sistemas de Liberação de Medicamentos , Feminino , Humanos , Lipossomos/administração & dosagem , Lipossomos/química , Camundongos , Camundongos Endogâmicos BALB C , Microesferas , Taxa de Sobrevida , Inibidores da Topoisomerase II/administração & dosagem , Inibidores da Topoisomerase II/química , Ensaios Antitumorais Modelo de Xenoenxerto
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