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1.
J Mater Chem B ; 10(24): 4595-4604, 2022 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-35642510

RESUMO

Nanoparticle-based tumor therapy strategies have been widely developed, while the therapeutic efficacy is often limited due to poor accumulation of nanoparticles in tumor tissues and low antitumor effect of sole therapeutic modality. In this study, we report the construction of tumor-targeting biomimetic sonosensitizer-conjugated iron oxide (Fe3O4) nanocatalysts to mediate combinational action of chemodynamic therapy (CDT) and sonodynamic therapy (SDT) for the treatment of colorectal cancer. Bovine serum albumin (BSA)-modified Fe3O4 nanoparticles were synthesized using a basic co-precipitation method, and were conjugated with chlorin e6 (Ce6) as the sonosensitizers, followed by surface camouflage of a CT26 cancer cell membrane to construct the tumor-targeting biomimetic nanocatalysts (MBFC). The obtained MBFC nanocatalysts could present a strong catalysis ability and efficient sonodynamic property to generate an abundant amount of reactive oxygen species (ROS) under ultrasound (US) treatment in the tumor microenvironment. Cellular internalization experiments verified the high cellular uptake efficacy of MBFC due to the cell membrane-mediated homologous targeting mechanism. The MBFC nanocatalysts enabled the combinational action of CDT and SDT, and could markedly induce the apoptosis of CT26 cells in vitro and greatly inhibit the growth of CT26 tumors in living mice. This study thus provides a tumor-targeting biomimetic nanoplatform for the effective therapy of tumors.


Assuntos
Neoplasias Colorretais , Terapia por Ultrassom , Animais , Biomimética , Linhagem Celular Tumoral , Neoplasias Colorretais/tratamento farmacológico , Compostos Férricos , Camundongos , Microambiente Tumoral , Terapia por Ultrassom/métodos
2.
Mil Med Res ; 9(1): 26, 2022 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-35676737

RESUMO

Glioblastoma multiforme (GBM) is the most common primary malignant brain tumor, and it is associated with poor prognosis. Its characteristics of being highly invasive and undergoing heterogeneous genetic mutation, as well as the presence of the blood-brain barrier (BBB), have reduced the efficacy of GBM treatment. The emergence of a novel therapeutic method, namely, sonodynamic therapy (SDT), provides a promising strategy for eradicating tumors via activated sonosensitizers coupled with low-intensity ultrasound. SDT can provide tumor killing effects for deep-seated tumors, such as brain tumors. However, conventional sonosensitizers cannot effectively reach the tumor region and kill additional tumor cells, especially brain tumor cells. Efforts should be made to develop a method to help therapeutic agents pass through the BBB and accumulate in brain tumors. With the development of novel multifunctional nanosensitizers and newly emerging combination strategies, the killing ability and selectivity of SDT have greatly improved and are accompanied with fewer side effects. In this review, we systematically summarize the findings of previous studies on SDT for GBM, with a focus on recent developments and promising directions for future research.


Assuntos
Neoplasias Encefálicas , Glioblastoma , Terapia por Ultrassom , Neoplasias Encefálicas/tratamento farmacológico , Neoplasias Encefálicas/patologia , Glioblastoma/terapia , Humanos , Terapia por Ultrassom/métodos , Ultrassonografia
3.
J Acoust Soc Am ; 151(5): 3043, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35649924

RESUMO

A polyurethane-based tissue mimicking material (TMM) and blood mimicking material (BMM) for the acoustic and thermal characterization of high intensity therapeutic ultrasound (HITU) devices has been developed. Urethane powder and other chemicals were dispersed into either a high temperature hydrogel matrix (gellan gum) or degassed water to form the TMM and BMM, respectively. The ultrasonic properties of both TMM and BMM, including attenuation coefficient, speed of sound, acoustical impedance, and backscatter coefficient, were characterized at room temperature. The thermal conductivity and diffusivity, BMM viscosity, and TMM Young's modulus were also measured. Importantly, the attenuation coefficient has a nearly linear frequency dependence, as is the case for most soft tissues and blood at 37 °C. Their mean values are 0.61f1.2 dB cm-1 (TMM) and 0.2f1.1 dB cm-1 (BMM) based on measurements from 1 to 8 MHz using a time delay spectrometry (TDS) system. Most of the other relevant physical parameters are also close to the reported values of soft tissues and blood. These polyurethane-based TMM and BMM are appropriate for developing standardized dosimetry techniques, validating numerical models, and determining the safety and efficacy of HITU devices.


Assuntos
Poliuretanos , Terapia por Ultrassom , Acústica , Imagens de Fantasmas , Viscosidade
4.
J Nanobiotechnology ; 20(1): 283, 2022 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-35710424

RESUMO

BACKGROUND: Sonodynamic therapy (SDT) induces immunogenic cell death (ICD) in tumors and promises to play an assistive role in immunotherapy in pancreatic cancer. However, the short half-life and limited diffusion distance of reactive oxygen species (ROS) impair ICD induction, especially in tumors with relatively poor blood perfusion and dense stroma. RESULTS: To address this problem, we fabricated cavitation-assisted endoplasmic reticulum (ER) targeted sonodynamic nanodroplets (PMPS NDs, 329 nm). The good sonodynamic effect and precise endoplasmic reticulum target effect was verified. After intravenous injection, the cRGD peptide modified nanodroplets initially aggregated around the tumor vascular endothelial cells. Stimulated by ultrasound, the liquid-to-gas bubbles began to oscillate and cavitate. This acoustic droplet evaporation strategy facilitated transport of the nanoparticle across the vessel, with deep penetration. This loosened the tumor stroma and facilitated accumulation and penetration of loaded sonosensitizer after 6 h. The modified sonosensitizer can selectively accumulate in the ER to generate a large amount of ROS in situ, inducing potent ER stress, amplified ICD and dendritic cell maturation in vitro and in vivo. Furthermore, the elevated antitumor effect of SDT plus anti-PD-L1 immunotherapy was verified using an orthotopic tumor model. CONCLUSIONS: This study reports a cavitation assisted ER targeted sonodynamic therapy that can enhance the effect of anti-PD-L1 immunotherapy effectively in orthotopic and distant pancreatic cancer.


Assuntos
Neoplasias Pancreáticas , Terapia por Ultrassom , Linhagem Celular Tumoral , Retículo Endoplasmático , Células Endoteliais/metabolismo , Humanos , Imunoterapia , Neoplasias Pancreáticas/tratamento farmacológico , Espécies Reativas de Oxigênio/metabolismo
5.
Adv Exp Med Biol ; 1364: 397-409, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35508885

RESUMO

Focused ultrasound holds great promise in therapy for its ability to target non-invasively deep seated tissues with non-ionizing therapeutic beams. Nevertheless, brain applications have been hampered for decades by the presence of the skull. The skull indeed strongly reflects, refracts and absorbs ultrasound, which defocuses the therapeutic ultrasound beams. In this chapter, we will first show how the structure of the skull impacts the ultrasound beams and how it narrows the frequency range that can be envisioned for transcranial therapy. We will then introduce different methods that have been developed and optimized to compensate the defocusing effect of the bone. Finally, we will provide an overview of past, current and future treatments of brain disorders.


Assuntos
Encefalopatias , Terapia por Ultrassom , Encéfalo/diagnóstico por imagem , Encefalopatias/diagnóstico por imagem , Encefalopatias/terapia , Humanos , Crânio/diagnóstico por imagem , Ultrassonografia
6.
Brain Stimul ; 15(3): 769-779, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35561960

RESUMO

Ultrasonic neurostimulation is a potentially potent noninvasive therapy, whose mechanism has yet to be elucidated. We designed a system capable of applying ultrasound with minimal reflections to neuronal cultures. Synaptic transmission was pharmacologically controlled, eliminating network effects, enabling examination of single-cell processes. Short single pulses of low-intensity ultrasound were applied, and time-locked responses were examined using calcium imaging. Low-pressure (0.35 MPa) ultrasound directly stimulated ∼20% of pharmacologically disconnected neurons, regardless of membrane poration. Stimulation was resistant to the blockade of several purinergic receptor and mechanosensitive ion channel types. Stimulation was blocked, however, by suppression of action potentials. Surprisingly, even extremely short (4 µs) pulses were effective, stimulating ∼8% of the neurons. Lower-pressure pulses (0.35 MPa) were less effective than higher-pressure ones (0.65 MPa). Attrition effects dominated, with no indication of compromised viability. Our results detract from theories implicating cavitation, heating, non-transient membrane pores >1.5 nm, pre-synaptic release, or gradual effects. They implicate a post-synaptic mechanism upstream of the action potential, and narrow down the list of possible targets involved.


Assuntos
Terapia por Ultrassom , Ultrassom , Potenciais de Ação/fisiologia , Neurônios/fisiologia , Terapia por Ultrassom/métodos , Ondas Ultrassônicas , Ultrassonografia
7.
Wiad Lek ; 75(3): 732-734, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35522886

RESUMO

Aortic stenosis (AS) - a constantly progressing disease characterized by thickening and calcification of leaflets of the valve, which leads to obstruction of the blood outflow from the left ventricular (LV), inadequate cardiac output, heart failure, and even sudden death. Prevalence of stenosis consistently increases with age, 0.2% incidence observes in the 50-59 year cohort of patients and almost 10% in patients from the 80-89 year cohort. We report the case of a 78-year-old man who presented with severe aortic valve stenosis. Since conventional valve replacement was problematic because of a small annular diameter we performed ultrasonic aortic valve decalcification. As a result, the systolic pressure gradient across the aortic valve decreased from 106 mm Hg to 22 mm Hg. Our case demonstrated that even in modern era ultrasonic aortic valve decalcification can be an effective option for restoration of mobility of the aortic valve cusps. We advocate the use of it for elderly patients with small aortic annulus in which surgical or transcatheter valve replacement, valve reconstruction could be extremely difficult or unavailable and consider ultrasonic valve decalcification as an additional tool in cardiac surgeon's hands.


Assuntos
Estenose da Valva Aórtica , Terapia por Ultrassom , Idoso , Valva Aórtica/diagnóstico por imagem , Valva Aórtica/cirurgia , Estenose da Valva Aórtica/diagnóstico por imagem , Estenose da Valva Aórtica/cirurgia , Constrição Patológica , Humanos , Recém-Nascido , Masculino , Resultado do Tratamento , Ultrassom
8.
Ultrasonics ; 124: 106751, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35512579

RESUMO

Given their advantage of suppressing grating lobes, randomly arranged linear arrays have potential for use in ultrasonic treatment. The current work proposes a method based on genetic algorithm to optimize the random arrangement of array elements, so that the suppression effect of grating lobes can be significantly improved with reduced calculating time. The maximum and average kerfs of array elements are used as genes, and the ratio of the maximum to the secondary maximum sound pressure at the focal plane is used as the optimized target. Typically, the calculation requirements of the current method can be reduced to ∼ 25% of the traversing method. We further discuss how the kerf width, the effective ratio of element areas and the ratio of focal distance to array aperture affect the suppression of grating lobes. For a typical linear array with 32 elements (1-MHz operating frequency, 1.5-mm element width and 150-mm focal distance), the results suggest that the grating lobes are suppressed well when (1) the ratio of maximum width to average width of the element is between 5 and 8, (2) the ratio of the effective element area to the area of the whole array is between 0.5 and 0.9, and (3) the ratio of the effective emission aperture to the actual emission aperture of the array is as large as possible. Based on optimized parameters, an experimental array was fabricated and the measured results of corresponding sound field were entirely consistent with the simulated results (Given her role as an Associate Editor of this journal, Juan Tu had no involvement in the peer-review of articles for which she was an author and had no access to information regarding the peer-review. Full responsibility for the peer-review process for this article was delegated to another Editor of this journal.).


Assuntos
Terapia por Ultrassom , Ultrassom , Algoritmos , Som , Transdutores , Terapia por Ultrassom/métodos
9.
Adv Exp Med Biol ; 1364: 411-422, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35508886

RESUMO

Skull bone is the main obstacle for transcranial ultrasound therapy and imaging applications. Most efforts in characterizing ultrasonic properties of the skull have been limited to a narrow frequency range and normal incidence. On the other hand, acoustic guided waves in plates have been used in non-destructive evaluation of materials and also to assess the strength of long bones. Recent work has likewise revealed the existence of skull-guided waves (SGWs) in mice and humans when performing measurements over a broad range of frequencies and incidence angles. Here we provide an overview on the recent progress in our understanding on the propagation of SGWs, describe the measurement techniques used to detect SGWs, the experimental observations, and the accompanying modeling efforts. Finally, the outstanding challenges to harness SGWs in applications such as transcranial therapy, imaging, and cranial bone assessment are discussed.


Assuntos
Crânio , Terapia por Ultrassom , Animais , Cabeça , Camundongos , Crânio/diagnóstico por imagem , Som
10.
Comput Math Methods Med ; 2022: 3921021, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35586674

RESUMO

Objective: To explore the effect of moxibustion instrument combined with ultrashort wave on pain and oxidative stress in elderly patients with knee osteoarthritis (KOA). Method: 84 elderly patients with knee osteoarthritis treated in our hospital from May 2020 to June 2021 were randomly divided into observation group (n = 42) and control group (n = 42). The observation group was treated with moxibustion instrument combined with ultrashort wave, while the control group was treated with moxibustion instrument. The clinical efficacy of the two groups was compared, and the pain of the two groups was evaluated by visual analogue scale (VAS). Lysholm knee joint score scale and osteoarthritis index (WOMAC) scale of Western Ontario and McMaster University were used to evaluate the knee joint function of the two groups, and the levels of interleukin-1ß (IL-1ß), tumor necrosis factor α (TNF-α), serum superoxide dismutase (SOD), serum malondialdehyde (MDA), serum miR-155, and NLRP3 were detected in the two groups, and the comprehensive quality of life assessment questionnaire-74 was used, and the adverse reactions were compared between the two groups. Results: The total effective rate of observation group (90.48%) was higher than that of control group (69.05%) (P < 0.05). After treatment, VAS, Lysholm knee joint, WOMAC, quality of life scores, IL-1ß, TNF-α, SOD, MDA, miR-155, and NLRP3 in the observation group were better than those in the control group, and the differences were statistically significant (P < 0.05). There were no obvious adverse reactions in both groups. Conclusion. Moxibustion instrument combined with ultrashort wave can effectively improve knee joint pain, knee joint function, inflammatory reaction, oxidative stress reaction, and quality of life in elderly KOA patients, and the therapeutic effect is good.


Assuntos
Moxibustão , Osteoartrite do Joelho , Terapia por Ultrassom , Fatores Etários , Idoso , Humanos , Articulação do Joelho , MicroRNAs , Proteína 3 que Contém Domínio de Pirina da Família NLR , Osteoartrite do Joelho/metabolismo , Osteoartrite do Joelho/terapia , Estresse Oxidativo , Dor/etiologia , Manejo da Dor , Qualidade de Vida , Superóxido Dismutase/metabolismo , Resultado do Tratamento , Fator de Necrose Tumoral alfa/metabolismo
11.
Ultrasound Med Biol ; 48(7): 1299-1308, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35461726

RESUMO

These recommendations are intended to provide guidance and to encourage best practice in reporting therapeutic ultrasound treatment parameters. Detailed uniform reporting will allow testing of therapy ultrasound systems and protocols, cross-comparison of studies between different teams using different systems and validation of therapeutic bio-effects. These recommendations have been divided into two sets, one for clinical and one for preclinical studies, each with stratified reporting categories, to account for the disparities in expertise and access to equipment between sites. The recommendations are intended to be useful for clinicians and researchers, for ethical and funding review boards and for the editors and reviewers of scientific journals.


Assuntos
Ablação por Ultrassom Focalizado de Alta Intensidade , Terapia por Ultrassom , Ablação por Ultrassom Focalizado de Alta Intensidade/métodos , Ultrassonografia
12.
Diabetes Metab Syndr ; 16(5): 102483, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35483209

RESUMO

BACKGROUND AND AIMS: Airborne Low Intensity Multi frequency Ultrasound (ALIMFUS) uses thermal and non thermal principal of ultrasound to facilitate transportation of drugs into the cells and it's metabolism. This is randomized, multi-center, Double Blind, Interventional, Placebo Controlled Study to evaluate efficacy and safety of ALIMFUS as an Add-on therapy to Oral Hypoglycemic Agent (OHA) in Type 2 DM. METHODS: Total 103/186 subjects completed the study and received 10 min either ALIMFUS therapy on alternate day for 90 days or placebo. Baseline and end of the study Lab parameters like HbA1c, blood sugars, Lipid Profile, Serum Hs-CRP, Serum Interleukin-6, Serum TNF-α, Serum homocysteine, Serum Vitamin D, Serum Leptin, Serum Adiponectin and Quality of Life score were assessed. RESULTS: At the end of study ALIMFUS group achieved greater (0.77 ± 1.13 vs 0.48 ± 0.79) but non-significant reduction in HbA1c. More subjects in ALIMFUS group (30.76% vs 27.45%) achieved HbA1c < 7%. Significant reduction in fasting and postprandial glucose noted in both groups whose baseline HbA1c was ≥8%. Significant reduction in lipid profile noted in ALIMFUS group compared to placebo. Insulin, adiponectin, CRP and homocysteine and quality of life were significantly better in ALMFUS group compared to baseline; but non-significant compared to placebo. No adverse events were associated with ALIMFUS. CONCLUSIONS: Thus, ALIMFUS could be novel technology in diabetes management for patient unable to achieve glycemic targets on combination therapy. However further exploratory long term studies are required to demonstrate its effective role as add-on therapy in diabetes management.


Assuntos
Diabetes Mellitus Tipo 2 , Hipoglicemiantes , Terapia por Ultrassom , Adiponectina/sangue , Glicemia/metabolismo , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/terapia , Método Duplo-Cego , Hemoglobina A Glicada/metabolismo , Controle Glicêmico , Humanos , Hipoglicemiantes/uso terapêutico , Metabolismo dos Lipídeos , Qualidade de Vida
13.
Int J Mol Sci ; 23(8)2022 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-35457210

RESUMO

At present, cancer is one of the leading causes of death worldwide. Treatment failure remains one of the prime hurdles in cancer treatment due to the metastatic nature of cancer. Techniques have been developed to hinder the growth of tumours or at least to stop the metastasis process. In recent years, ultrasound therapy combined with microbubbles has gained immense success in cancer treatment. Ultrasound-stimulated microbubbles (USMB) combined with other cancer treatments including radiation therapy, chemotherapy or immunotherapy has demonstrated potential improved outcomes in various in vitro and in vivo studies. Studies have shown that low dose radiation administered with USMB can have similar effects as high dose radiation therapy. In addition, the use of USMB in conjunction with radiotherapy or chemotherapy can minimize the toxicity of high dose radiation or chemotherapeutic drugs, respectively. In this review, we discuss the biophysical properties of USMB treatment and its applicability in cancer therapy. In particular, we highlight important preclinical and early clinical findings that demonstrate the antitumour effect combining USMB and other cancer treatment modalities (radiotherapy and chemotherapy). Our review mainly focuses on the tumour vascular effects mediated by USMB and these cancer therapies. We also discuss several current limitations, in addition to ongoing and future efforts for applying USMB in cancer treatment.


Assuntos
Neoplasias , Terapia por Ultrassom , Linhagem Celular Tumoral , Terapia Combinada , Microbolhas , Neoplasias/terapia , Terapia por Ultrassom/métodos , Ultrassonografia
14.
J Nanobiotechnology ; 20(1): 186, 2022 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-35413839

RESUMO

BACKGROUND: Sonodynamic therapy (SDT) is a burgeoning modality for cancer therapy owing to its high tissue-penetrating capability, controllability and safety. Whereas, the undesirable reactive oxygen species (ROS) yield of sonosensitizers and tumor hypoxia are two vulnerable spots of SDT. Therefore, it is an advisable strategy to augment ROS level and simultaneously relieve hypoxia for SDT to arrive its full potential in cancer treatment. RESULTS: In this work, the defected two-dimensional (2D) Pd/H-TiO2 nanosheets (NSs) with triple antineoplastic properties were dexterously elaborated and engineered using a facile one-pot Pd-catalyzed hydrogenation tactic by loading a tiny amount of Pd and then inletting hydrogen flow at atmospheric pressure and temperature. The 2D black Pd/H-TiO2 NSs with oxygen defects exerted eximious SDT effect based on the decreased bandgap that made it easier for the separation of electrons and holes when triggered by ultrasound as theoretically guided by density functional theory calculations. Additionally, Pd/H-TiO2 NSs could serve as Fenton-like agents because of the presence of oxygen defects, facilitating the conversion of hydrogen peroxide into hydroxyl radicals for exerting the chemodynamic therapy (CDT). Simultaneously, the introduced tiny Pd component possessed catalase-like activity responsible for oxygen production to ameliorate hypoxic condition and thus contributed to improving SDT and CDT efficacies. Both in vitro and in vivo results provided compelling evidences of high ROS yield and aggrandized sono-chemodynamic effect of Pd/H-TiO2 nanosonosensitizers with the detailed underlying mechanism investigation by RNA sequencing. CONCLUSION: This work delves the profound potential of Pd-catalyzed hydrogenated TiO2 on oncotherapy, and the effective antineoplastic performance and ignorable therapeutic toxicity make it a powerful competitor among a cornucopia of nanosonosensitizers.


Assuntos
Antineoplásicos , Neoplasias , Terapia por Ultrassom , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Linhagem Celular Tumoral , Humanos , Hipóxia/tratamento farmacológico , Neoplasias/tratamento farmacológico , Oxigênio , Paládio/farmacologia , Paládio/uso terapêutico , Espécies Reativas de Oxigênio , Titânio , Terapia por Ultrassom/métodos
15.
Biosensors (Basel) ; 12(4)2022 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-35448315

RESUMO

Upon harnessing low-intensity ultrasound to activate sonosensitizers, sonodynamic therapy (SDT) induces cancer cell death through the reactive oxygen species (ROS) mediated pathway. Compared with photodynamic therapy (PDT), SDT possesses numerous advantages, including deeper tissue penetration, higher accuracy, fewer side effects, and better patient compliance. Sinoporphyrin sodium (DVDMS), a sonosensitizer approved by the FDA, has drawn abundant attention in clinical research, but there are some deficiencies. In order to further improve the efficiency of DVDMS, many studies have applied self-assembly nanotechnology to modify it. Furthermore, the combined applications of SDT/chemodynamic therapy (CDT) have become a research hotspot in tumor therapy. Therefore, we explored the self-assembly of nanoparticles based on DVDMS and copper to combine SDT and CDT. A cost-effective sonosensitizer was synthesized by dropping CuCl2 into the DVDMS solution with the assistance of PVP. The results revealed that the nanostructures could exert excellent treatment effects on tumor therapy and perform well for PET imaging, indicating the potential for cancer theranostics. In vitro and in vivo experiments showed that the nanoparticles have outstanding biocompatibility, higher ROS production efficiency, and antitumor efficacy. We believe this design can represent a simple approach to combining SDT and CDT with potential applications in clinical treatment and PET imaging.


Assuntos
Nanopartículas , Fotoquimioterapia , Porfirinas , Terapia por Ultrassom , Linhagem Celular Tumoral , Humanos , Porfirinas/química , Espécies Reativas de Oxigênio , Terapia por Ultrassom/métodos
16.
J Neurooncol ; 157(2): 237-247, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35267132

RESUMO

PURPOSE: Six years ago, in 2015, the Focused Ultrasound Foundation sponsored a workshop to discuss, and subsequently transition the landscape, of focused ultrasound as a new therapy for treating glioblastoma. METHODS: This year, in 2021, a second workshop was held to review progress made in the field. Discussion topics included blood-brain barrier opening, thermal and nonthermal tumor ablation, immunotherapy, sonodynamic therapy, and desired focused ultrasound device improvements. RESULTS: The outcome of the 2021 workshop was the creation of a new roadmap to address knowledge gaps and reduce the time it takes for focused ultrasound to become part of the treatment armamentarium and reach clinical adoption for the treatment of patients with glioblastoma. Priority projects identified in the roadmap include determining a well-defined algorithm to confirm and quantify drug delivery following blood-brain barrier opening, identifying a focused ultrasound-specific microbubble, exploring the role of focused ultrasound for liquid biopsy in glioblastoma, and making device modifications that better support clinical needs. CONCLUSION: This article reviews the key preclinical and clinical updates from the workshop, outlines next steps to research, and provides relevant references for focused ultrasound in the treatment of glioblastoma.


Assuntos
Glioblastoma , Terapia por Ultrassom , Barreira Hematoencefálica , Sistemas de Liberação de Medicamentos , Glioblastoma/tratamento farmacológico , Glioblastoma/terapia , Humanos , Microbolhas
17.
Ultrasound Med Biol ; 48(6): 1078-1094, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35304006

RESUMO

To the best of our knowledge, therapeutic ultrasound (TUS) is thus far an unexplored means of delivering mechanical stimulation to cardiomyocyte cultures, which is necessary to engineer a more mature cardiomyocyte phenotype in vitro. Spectral ultrasound (SUS) may provide a way to non-invasively, non-disruptively and inexpensively monitor growth and change in cell cultures over long periods. Compared with other measurement methods, SUS as an acoustic measurement tool will not be affected by an acoustic therapy, unlike electrical measurement methods, in which motion caused by acoustic therapy can affect measurements. Further SUS has the potential to provide functional as well as morphological information in cell cultures. Human induced pluripotent stem cell cardiomyocytes (iPS-CMs) were imaged with calcium fluorescence microscopy while TUS was being applied. TUS was applied at 600 kHz and 1, 3.4 and 6 W/cm2 for a continuous 1 s pulse. Measures of the instantaneous beat frequency, repolarization rate and calcium spike amplitude were calculated from the fluorescence data. At 600 kHz, TUS at 1 and 6 W/cm2 had significant effects on the shortening of both the repolarization rate and instantaneous beat rate of the iPS-CMs (p < 0.05), while TUS at 3.4 and 6 W/cm2 had significant effects on the shortening of the calcium spike amplitude (p < 0.05). Three SUS measures and one gray-level measure were captured from the iPS-CM monolayers while they were simultaneously being imaged with calcium-labeled confocal microscopy. The gray-level measure performed the best of all SUS measures; however, it was not reliable enough to produce a consistent determination of the beat rate of the cell. Finally, SUS measures were captured using three different transducers while simultaneously applying TUS. A center-of-mass (COM) measure calculated from the wavelet transform scalogram of the time-averaged radiofrequency data revealed that SUS was able to detect a change in the frequency content of the reflected ultrasound at 1 and 6 W/cm2 before and after ultrasound application (p < 0.05), showing promise for the ability of SUS to measure changes in the beating behavior of iPS-CMs. Overall, SUS is promising as a method for constant monitoring of dynamic cell and tissue culture and growth.


Assuntos
Células-Tronco Pluripotentes Induzidas , Terapia por Ultrassom , Cálcio , Humanos , Miócitos Cardíacos , Ultrassonografia
18.
Int J Med Robot ; 18(3): e2389, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35257476

RESUMO

BACKGROUND: High-quality methods for Magnetic Resonance guided Focussed Ultrasound (MRgFUS) therapy planning are needed for safe and efficient clinical practices. Herein, an algorithm for full coverage path planning based on preoperative MR images is presented. METHODS: The software functionalities of an MRgFUS robotic system were enhanced by implementing the developed algorithm. The algorithm's performance in accurate path planning following a Zig-Zag pathway was assessed on MR images. The planned sonication paths were performed on acrylic films using the robotic system carrying a 2.75 MHz single element transducer. RESULTS: Ablation patterns were successfully planned on MR images and produced on acrylic films by overlapping lesions with excellent match between the planned and experimental lesion shapes. CONCLUSIONS: The advanced software was proven efficient in planning and executing full ablation of any segmented target. The reliability of the algorithm could be enhanced through the development of a fully automated segmentation procedure.


Assuntos
Imageamento por Ressonância Magnética , Terapia por Ultrassom , Algoritmos , Humanos , Imageamento por Ressonância Magnética/métodos , Espectroscopia de Ressonância Magnética , Reprodutibilidade dos Testes , Terapia por Ultrassom/métodos
19.
J Pak Med Assoc ; 72(3): 522-525, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35320236

RESUMO

OBJECTIVE: To systematically review the role of low-intensity pulsed ultrasound on lumbar spondylolysis. METHODS: Literature search was conducted on PubMed, Embase, CINAHL, Web of Science, PEDro and Scopus databases to identify relevant studies published between 2010 and 2020 by using medical subject headings and applying Booleans, such as low-intensity pulsed ultrasound OR interventional ultrasound AND lumbar spine OR lumbar region AND spondylolysis OR stress fracture. Unpublished studies were hand-searched in the journals, abstracts of conferences were reviewed, and citation index was used for searching experts in the field and then contacting them for information. Studies included were the ones that had at least one of the following outcomes: bone union rate, treatment period to bone union and time to return to previous activities. RESULTS: Of the 243 studies identified, 228(94%) were full text articles and only 2(0.8%) studies were critically appraised for qualitative synthesis based on bone union rate, treatment period to bone union, and time to return to previous activities. CONCLUSIONS: Low-intensity pulsed ultrasound was found to be effective for bone union and a useful therapy for quick return to playing sports in patients with lumbar spondylolysis.


Assuntos
Espondilólise , Terapia por Ultrassom , Mãos , Humanos , Região Lombossacral , Espondilólise/diagnóstico por imagem , Espondilólise/terapia , Ondas Ultrassônicas
20.
Ultrason Sonochem ; 84: 105972, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35255361

RESUMO

Among various novel antimicrobial therapies, sonodynamic therapy (SDT) exhibits its advantages for the treatment of bacterial infections due to its high penetration depth and low side effects. In this study, a new nanosonosensitizer (HFH@ZIF-8) that loads sonosensitizer hematoporphyrin monomethyl ether (HMME) into zeolitic imidazolate framework-8 (ZIF-8), was constructed for killing multidrug-resistant (MDR) bacteria and treatment of in vivo infection diseases by SDT. In particular, the developed HFH@ZIF-8 exhibited enhanced water-solubility, good biocompatibility, and improved disease-targeting capability for delivering and releasing HMME and ablating the infected lesion. More importantly, the presence of oxygen-carrying hemoglobin for HFH@ZIF-8 can offer sufficient oxygen consumption by SDT, augmenting the efficacy of SDT by improving ROS generating efficiency against deep tissue multidrug-resistant bacterial infection. Therefore, this study paves a new avenue for treating infection disease, particularly for antibiotic resistant bacterial infection.


Assuntos
Terapia por Ultrassom , Bactérias , Biomimética , Linhagem Celular Tumoral , Oxigênio
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