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1.
Exp Parasitol ; 191: 88-96, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30009810

RESUMO

Schistosomiasis remains a global health problem. In the Mekong river basin, approximately 80,000 people are at risk of infection by Schistosoma mekongi. The parasite's eggs become entrapped in the host's organs and induce massive inflammation, contributing to the pathogenesis of schistosomiasis. In addition, egg antigens are important in circumoval precipitin tests (COPTs) and other diagnostic techniques. Little is known regarding the egg proteins of S. mekongi, and so we applied immunoblotting and mass spectrometry-based proteomic approaches to study these proteins and their antigenicity. A total of 360 unique proteins were identified in S. mekongi eggs using proteomic analyses. The major protein components of S. mekongi eggs were classified into several groups by functions, including proteins of unknown function, structural proteins, and regulators of transcription and translation. The most abundant proteins in S. mekongi eggs were antioxidant proteins, potentially reflecting the need to neutralize reactive oxidative species released from host immune cells. Immunomic analyses revealed that only DNA replication factor Cdt1 and heat shock protein 70 overlap between the proteins recognized by sera of infected mice and humans, illustrating the challenges of knowledge transfer from animal models to human patients. Forty-one immunoreactive protein bands were recognized by either mouse or patient sera. Phosphoglycerate kinase, fructose-1,6-bisphosphate aldolase and elongation factor 1 appeared to be interesting immunogens of S. mekongi eggs as these proteins were recognized by polyclonal IgMs and IgGs in patient sera. Our findings provide new information on the protein composition of S. mekongi eggs as well as the beginnings of a S. mekongi immunogen dataset. These data may help us better understand the pathology of schistosomiasis as well as natural antibody responses against S. mekongi egg proteins, both of which may be useful in including S. mekongi to other schistosoma diagnostic, vaccine and immunotherapy development.


Assuntos
Proteínas de Helminto/química , Proteoma/análise , Proteômica , Schistosoma/química , Schistosoma/imunologia , Animais , Antígenos de Helmintos/análise , Antígenos de Helmintos/imunologia , Antioxidantes/análise , Estudos de Casos e Controles , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Gastrópodes , Proteínas de Helminto/análise , Proteínas de Helminto/imunologia , Humanos , Soros Imunes/imunologia , Immunoblotting , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Vale do Mecom/epidemiologia , Camundongos , Camundongos Endogâmicos ICR , Óvulo/química , Óvulo/imunologia , Testes de Precipitina , Proteoma/química , Proteoma/imunologia , Esquistossomose/epidemiologia , Esquistossomose/imunologia , Esquistossomose/parasitologia , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem
2.
Rev Soc Bras Med Trop ; 50(4): 543-546, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28954078

RESUMO

INTRODUCTION:: Studying the feeding preferences of triatomines is an important entomological surveillance tool, since continuous surveillance of the disease is necessary. METHODS:: The precipitin reaction was used to describe the feeding preferences of triatomines along with their natural infection by flagellates similar to Tyrpanosoma cruzi. Six hundred eighty-seven insects were examined, including Triatoma brasiliensis, Triatoma pseudomaculata, and Panstrongylus lutzi. RESULTS:: Sixty-nine (10%) of 687 triatomines examined tested positive for flagellates similar to T. cruzi, and 8 (1.2%) of these fed on human blood. CONCLUSIONS:: This study found potential transmitters of Chagas disease both inside and outside the domiciliar environment.


Assuntos
Comportamento Alimentar/fisiologia , Conteúdo Gastrointestinal , Insetos Vetores/fisiologia , Triatominae/fisiologia , Animais , Brasil , Doença de Chagas/transmissão , Feminino , Insetos Vetores/classificação , Masculino , Testes de Precipitina , Triatominae/classificação
3.
Respir Med ; 129: 173-178, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28732828

RESUMO

BACKGROUND: The diagnostic evaluation of patients with interstitial lung disease (ILD) often involves serologic assessment for identifiable causes such as hypersensitivity pneumonitis (HP). While not on its own defining of HP, precipitin serologies are often obtained to support clinical suspicion if other findings are inconclusive. We studied the clinical relevance of positive avian serology in patients undergoing ILD evaluation. MATERIAL AND METHODS: We identified individuals with positive avian serology (>53.3 mg/L) and undifferentiated ILD seen at our institution over a three-year period. Clinical, laboratory, pathologic, and radiologic findings were evaluated for consensus HP diagnosis by two expert pulmonologists, blinded to presenting serology levels. RESULTS: Ninety-one ILD subjects with positive avian serology were identified; mean age was 62.7 ± 15.3 years with a slight male predominance (56%). Forty-nine (54%) received a consensus HP diagnosis. Those with HP had higher mean avian serology titer (95.0 ± 38.7 mg/L vs. 68.3 ± 16.7, (P < 0.0001). Never-smokers also had higher titers compared to prior or active smokers (P = 0.0008). Positive avian protein exposure (P < 0.0001, OR 21.3 (6.4-87)), DLCO% (P = 0.04, unit OR 0.96 (0.92-0.99)), and increasing serology titer (P < 0.015, unit OR 1.03 [1.01-1.06]) were independent predictors of HP diagnosis. CONCLUSION: Among patients with positive avian serology, those with higher titers were more likely to have HP diagnosis. Nonsmokers also manifested higher titers compared to those with smoking history. These results may guide the usage and interpretation of avian serology screening in the initial assessment of suspected HP.


Assuntos
Alveolite Alérgica Extrínseca/imunologia , Pulmão dos Criadores de Aves/imunologia , Exposição por Inalação/efeitos adversos , Doenças Pulmonares Intersticiais/diagnóstico , Doenças Pulmonares Intersticiais/imunologia , Idoso , Idoso de 80 Anos ou mais , Alveolite Alérgica Extrínseca/complicações , Alveolite Alérgica Extrínseca/diagnóstico por imagem , Alveolite Alérgica Extrínseca/fisiopatologia , Animais , Proteínas Aviárias/análise , Aves/imunologia , Lavagem Broncoalveolar , Feminino , Humanos , Doenças Pulmonares Intersticiais/etiologia , Masculino , Pessoa de Meia-Idade , Testes de Precipitina/métodos , Testes de Função Respiratória , Tomógrafos Computadorizados
4.
PLoS One ; 12(6): e0178263, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28614400

RESUMO

BACKGROUND: Farmer's lung (FL) is a common type of hypersensitivity pneumonitis. It is often considered that fibrosis is the most frequent finding in chronic FL. Nevertheless, three cohort studies have suggested that some patients with chronic FL may develop emphysema. We aimed to evaluate the current prevalence of emphysema in active FL, to describe the radiological and functional features of emphysema in active FL, and to identify risk factors associated with emphysema in this population. METHODS: Patients aged over 18 years with active FL were prospectively recruited through the SOPHIA study (CPP Est; P-2009-521), between 2007 and 2015. Each patient had complete medical history screening, clinical examination, high resolution computed tomodensitometry, bronchoalveolar lavage, pulmonary function tests and serum precipitins. RESULTS: Among 33 patients with active FL, the prevalence of emphysema in this series of incident active FL cases was higher (48.5%) than that of fibrosis (12%) and was not dependent on smoking habits. Most patients with emphysema did not have lung hyperinflation. The possible risk factors for emphysema in active FL were a longer duration of exposure to organic dusts, and at a higher level. CONCLUSION: Emphysema is found in half of patients with active FL and may be influenced by exposure patterns.


Assuntos
Pulmão de Fazendeiro/complicações , Enfisema Pulmonar/diagnóstico , Enfisema Pulmonar/epidemiologia , Idoso , Animais , Lavagem Broncoalveolar , Poeira/análise , Pulmão de Fazendeiro/fisiopatologia , Feminino , Fibrose , Abrigo para Animais , Humanos , Masculino , Pessoa de Meia-Idade , Testes de Precipitina , Estudos Prospectivos , Testes de Função Respiratória , Fatores de Risco , Tomografia Computadorizada por Raios X
5.
Exp Parasitol ; 179: 36-42, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28655585

RESUMO

Schistosomiasis is a chronic parasitic disease caused by trematodes of the genus Schistosoma, endemic in tropical and subtropical regions. The hepatic pathology of this parasitic disease could develop complications, such as fibrosis and cirrhosis, which can be fatal. The Venezuelan endemic area is considered as one of low transmission, which complicates the detection of infected individuals and signals the importance of improving the sensitivity of immunodiagnostic methods. Using ELISA, an evaluation was conducted of IgM and IgG responses to soluble antigens of eggs and female worms (SEA and SFWA) and excretion-secretion products of eggs and female worms (ESPE and ESPAW) in infected Balb/c mice with different parasitic burden and infection times. A high positivity rate by IgM detection was observed for all antigen preparations in 7-week infections (100% by SEA, SFWA, ESPE, and ESPWA in high parasitic burden) as well as a reduction of this immunoglobulin in chronic infection. Positivity rate for IgG detection was higher in 20-week infections (100% by ESPE in low burden, 100% by SEA and ESPE in medium burden, and 100% by ESPE and ESPAW in high burden conditions). The potential use of combined or unique antigenic preparations associated with IgM or IgG for detection of active infection, regardless the parasitic burden, was demonstrated. Differences between immunoglobulin responses show its application for phase-specific diagnosis.


Assuntos
Anticorpos Anti-Helmínticos/biossíntese , Imunoglobulina G/biossíntese , Imunoglobulina M/biossíntese , Schistosoma mansoni/imunologia , Esquistossomose mansoni/imunologia , Animais , Anticorpos Anti-Helmínticos/sangue , Cricetinae , Ensaio de Imunoadsorção Enzimática , Fezes/parasitologia , Feminino , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Mesocricetus , Camundongos , Camundongos Endogâmicos BALB C , Testes de Precipitina , Esquistossomose mansoni/diagnóstico , Esquistossomose mansoni/parasitologia
6.
Biomed Res Int ; 2015: 135689, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26504777

RESUMO

Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82-95.5%), differed significantly from COPT in positivity (P < 0.05), and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs.


Assuntos
Técnicas de Laboratório Clínico/métodos , Técnicas de Laboratório Clínico/estatística & dados numéricos , Doenças Endêmicas/estatística & dados numéricos , Imunoensaio/métodos , Esquistossomose mansoni/diagnóstico , Esquistossomose mansoni/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Brasil/epidemiologia , Criança , Pré-Escolar , Feminino , Humanos , Imunoensaio/estatística & dados numéricos , Lactente , Masculino , Pessoa de Meia-Idade , Vigilância da População/métodos , Testes de Precipitina/métodos , Testes de Precipitina/estatística & dados numéricos , Prevalência , Reprodutibilidade dos Testes , Medição de Risco/métodos , Esquistossomose mansoni/parasitologia , Sensibilidade e Especificidade , Adulto Jovem
7.
Biomed Res Int ; 2015: 1-16, 2015. ilus
Artigo em Inglês | LILACS, Sec. Est. Saúde SP, SESSP-IALPROD, Sec. Est. Saúde SP, SESSP-IALACERVO | ID: biblio-1022429

RESUMO

Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82­95.5%), differed significantly from COPT in positivity , and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs.


Assuntos
Esquistossomose mansoni/diagnóstico , Esquistossomose mansoni/parasitologia , Esquistossomose mansoni/epidemiologia , Idoso de 80 Anos ou mais , Brasil/epidemiologia , Idoso , Humanos , Imunoensaio/métodos , Imunoensaio/estatística & dados numéricos , Testes de Precipitina/métodos , Criança , Pré-Escolar , Vigilância da População/métodos , Prevalência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Medição de Risco/métodos , Adulto , Técnicas de Laboratório Clínico/métodos , Técnicas de Laboratório Clínico/estatística & dados numéricos , Doenças Endêmicas/estatística & dados numéricos , Adulto Jovem , Lactente , Pessoa de Meia-Idade
8.
J. bras. nefrol ; 36(4): 496-501, Oct-Dec/2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-731149

RESUMO

Introduction: Polyphenols contained in natural sources such as grapes, have been considered pharmacological agents to combat oxidative stress and inflammation, common features in Chronic Kidney Disease patients. Objective: To evaluate the effects of grape powder supplementation on inflammatory and antioxidant biomarkers in hemodialysis (HD) patients. Methods: The double-blind placebo-controlled randomized clinical trial evaluated non-diabetic HD patients that received grape powder (500 mg of polyphenols/day) (n = 16, 9 men, 53.0 ± 9.8 years of age, 111.6 ± 58.2 HD months) or placebo (n = 16, 9 men, 52.7 ± 13.7 years of age, 110.4 ± 93.1 HD months) for five weeks. The glutathione peroxidase (GSH-Px) activity and C-reactive protein (CRP) levels were evaluated by ELISA method. Results: After the intervention period, the patients receiving grape powder showed an increase in the GSH-Px activity (16.5 (41.0) to 42.0 (43.3) nmol/min/ml) (p < 0.05) and they did not have the CRP levels increased as seen in placebo group (2.6 (0.28) to 2.8 (0.23 mg/L) (p < 0.05). Conclusion: The use of grape powder as phenolic source could play an important role as an antioxidant and anti-inflammatory agent in non-diabetic HD patients. .


Introdução: Polifenóis contidos em fontes naturais, como as uvas, têm sido considerados agentes farmacológicos no combate ao estresse oxidativo e inflamação, condições comuns na Doença Renal Crônica. Objetivo: Avaliar os efeitos da suplementação de farinha de uva sobre marcadores inflamatórios e antioxidantes em pacientes submetidos à hemodiálise (HD). Métodos: Estudo randomizado, duplo-cego, placebocontrolado, no qual foram avaliados pacientes não diabéticos em HD que receberam farinha de uva (500 mg de polifenóis/dia) (n = 16, 9 homens, 53,0 ± 9,8 anos, 111,6 ± 58,2 meses em HD) ou placebo (n = 16, 9 homens, 52,7 ± 13,7 anos, 110,4 ± 93,1 meses em HD) por cinco semanas. A atividade da glutationa peroxidase (GSH-Px) e os níveis plasmáticos de proteína C-reativa (PCR) foram mensurados por meio do método ELISA. Resultados: Após o período de intervenção, os pacientes que receberam farinha de uva apresentaram elevação na atividade da GSH-Px (16,5 (41,0) para 42,0 (43,3) nmol/min/ml) (p < 0,05) e não foi observada elevação nos níveis de PCR, como visto no grupo placebo (2,6 (0,28) para 2,8 (0,23) mg/L) (p < 0,05). Conclusão: O uso da farinha de uva como fonte de polifenóis pode desempenhar um importante papel anti-inflamatório e antioxidante em pacientes não diabéticos submetidos à HD. .


Assuntos
Humanos , Proteínas de Ligação a DNA , Regulação da Expressão Gênica , Mutação , Proteínas Nucleares , Transativadores/metabolismo , Fatores de Transcrição/metabolismo , Sítios de Ligação , Carcinoma Hepatocelular , DNA Viral/metabolismo , Fator 1 Nuclear de Hepatócito , Fator 1-alfa Nuclear de Hepatócito , Fator 1-beta Nuclear de Hepatócito , Vírus da Hepatite B/genética , Vírus da Hepatite B/metabolismo , Testes de Precipitina , Plasmídeos/genética , Precursores de Proteínas/genética , Precursores de Proteínas/metabolismo , Transfecção , Células Tumorais Cultivadas , Transativadores/genética , Fatores de Transcrição/genética , Proteínas do Core Viral/genética , Proteínas do Core Viral/metabolismo
9.
Exp Parasitol ; 143: 83-9, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24858959

RESUMO

An experimental study in hamsters was performed to evaluate the capability for detecting Schistosoma mansoni DNA in serum and fecal samples during the pre and post-egg-laying periods of infection using TaqMan® Real-Time PCR system (qPCR), was compared with the circumoval precipitin test (COPT) and the Kato-Katz technique, especially among individuals with low parasitic burden. Twenty-four hamsters were infected with cercariae. Three hamsters were sacrificed per week under anesthesia, from 7 days post infection (DPI) up to 56 DPI. A serum sample and a pool of feces were collected from each hamster. The presence of S. mansoni eggs in fecal samples was evaluated by Kato-Katz method and in the hamsters gutby histopathology. Detection of S. mansoni DNA was performed using qPCR and S. mansoni antibody using COPT. The first detection of eggs in feces by Kato-Katz method and S. mansoni DNA in feces by qPCR occurred 49 DPI. Nevertheless, S. mansoni DNA was detected in serum samples from 14 up to 56 DPI. COPT was positive at 35 DPI. The results not only confirm the reliability of S. mansoni DNA detection by qPCR, but also demonstrate that serum is a trustworthy source of DNA in the pre patent infection period.


Assuntos
DNA de Helmintos/análise , Reação em Cadeia da Polimerase em Tempo Real , Schistosoma mansoni/isolamento & purificação , Esquistossomose mansoni/diagnóstico , Animais , Biomphalaria , Cricetinae , DNA de Helmintos/sangue , DNA de Helmintos/isolamento & purificação , Modelos Animais de Doenças , Fezes/parasitologia , Intestinos/parasitologia , Intestinos/patologia , Rim/parasitologia , Rim/patologia , Fígado/parasitologia , Fígado/patologia , Pulmão/parasitologia , Pulmão/patologia , Masculino , Testes de Precipitina , Schistosoma mansoni/genética , Esquistossomose mansoni/patologia , Sensibilidade e Especificidade , Baço/parasitologia , Baço/patologia
10.
Am J Trop Med Hyg ; 90(6): 1146-52, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24639303

RESUMO

Parasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective of this study was to determine the prevalence of Schistosoma mansoni infections by antibody detection using the indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples of 572 individuals were randomly selected. The IFA-IgM and COPT were used to detect anti-S. mansoni antibodies. Of the patients studied, 15.9% (N = 91) were IFA-IgM positive and 5.1% (N = 29) had COPT reactions (P < 0.001 by McNemar's test). Immunodiagnostic techniques showed higher infection prevalence than had been previously estimated. This study suggests that combined use of these diagnostic tools could be useful for the diagnosis of schistosomiasis in epidemiological studies in areas of low endemicity.


Assuntos
Anticorpos Anti-Helmínticos/sangue , Técnica Indireta de Fluorescência para Anticorpo/métodos , Testes de Precipitina/métodos , Schistosoma mansoni/imunologia , Esquistossomose mansoni/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Brasil , Criança , Pré-Escolar , Estudos Transversais , Fezes/parasitologia , Feminino , Humanos , Imunoglobulina M/sangue , Lactente , Masculino , Pessoa de Meia-Idade , Schistosoma mansoni/isolamento & purificação , Esquistossomose mansoni/parasitologia , Estudos Soroepidemiológicos , Adulto Jovem
11.
Indian J Exp Biol ; 52(12): 1182-5, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25651611

RESUMO

Two antigens ('cattle' type and 'Indian Bison' type) of Mycobacterium avium subspecies paratuberculosis were evaluated for diagnosis of Johne's disease (JD) in a gaushala (cattle herd). Of the 160 cows of Sahiwal and Hariana breeds screened, 81 (50.6%) tested positive in ELISA and 66 (41.8%) in AGPT test. Using the two antigens, 33.5% tested positive in both the tests while 41.1% tested negative. Exclusively, only 8.2% tested positive in ELISA while 17.1% tested positive in AGPT. Two antigens together detected 58.9% prevalence of MAP in the gaushala. Individually, indigenous ELISA using antigen from native source of MAP proved superior to AGPT in the diagnosis of JD in cows.


Assuntos
Antígenos de Bactérias/imunologia , Doenças dos Bovinos/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Mycobacterium avium subsp. paratuberculosis/imunologia , Paratuberculose/imunologia , Testes de Precipitina/métodos , Animais , Bison , Bovinos , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/microbiologia , Genótipo , Interações Hospedeiro-Patógeno/imunologia , Mycobacterium avium subsp. paratuberculosis/genética , Mycobacterium avium subsp. paratuberculosis/fisiologia , Paratuberculose/diagnóstico , Paratuberculose/microbiologia , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
12.
Rev Soc Bras Med Trop ; 46(5): 560-5, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24270247

RESUMO

INTRODUCTION: The blood meal source of sandflies provides valuable information about the vector/host interaction and allows for an understanding of American cutaneous leishmaniasis (ACL) transmission mechanisms. The aim of this study was to identify the blood meal sources of Lutzomyia (Nyssomyia) intermedia in an endemic area of leishmaniasis in Brazil's State of Paraná using a precipitin test. METHODS: Sandflies were collected in the rural locality of Epitácio Pessoa within the City of Adrianópolis, State of Paraná, in southern Brazil. A total of 864 female sandflies were captured, and 862 (99.8%) were identified as L. intermedia species. However, two unidentified specimens were considered to be part of the genus Lutzomyia. RESULTS: Among the females examined, 396 specimens presented reactions to a certain type of tested antiserum, and most (67.9%) reacted to the simple type. These sandflies fed mainly on the blood of birds, opossums, and rodents, but specimens that fed on the blood of humans, dogs, horses, cattle, and cats were also found. Among the cross-reactions found (32.1%), bird/rodent, bird/opossum, bird/dog, bird/human, and horse/dog cross-reactions were the most common. CONCLUSIONS: These results demonstrate a tendency in the eclectic feeding behavior of L. intermedia and support its potential role as a vector for ACL in the study area.


Assuntos
Insetos Vetores/fisiologia , Psychodidae/fisiologia , Animais , Aves , Brasil , Gatos , Bovinos , Cães , Comportamento Alimentar/fisiologia , Feminino , Cavalos , Humanos , Insetos Vetores/classificação , Leishmaniose Cutânea/transmissão , Gambás , Testes de Precipitina , Psychodidae/classificação , Roedores
13.
Rev. Soc. Bras. Med. Trop ; 46(5): 560-565, Sept-Oct/2013. tab, graf
Artigo em Inglês | LILACS | ID: lil-691416

RESUMO

Introduction The blood meal source of sandflies provides valuable information about the vector/host interaction and allows for an understanding of American cutaneous leishmaniasis (ACL) transmission mechanisms. The aim of this study was to identify the blood meal sources of Lutzomyia (Nyssomyia) intermedia in an endemic area of leishmaniasis in Brazil's State of Paraná using a precipitin test. Methods Sandflies were collected in the rural locality of Epitácio Pessoa within the City of Adrianópolis, State of Paraná, in southern Brazil. A total of 864 female sandflies were captured, and 862 (99.8%) were identified as L. intermedia species. However, two unidentified specimens were considered to be part of the genus Lutzomyia. Results Among the females examined, 396 specimens presented reactions to a certain type of tested antiserum, and most (67.9%) reacted to the simple type. These sandflies fed mainly on the blood of birds, opossums, and rodents, but specimens that fed on the blood of humans, dogs, horses, cattle, and cats were also found. Among the cross-reactions found (32.1%), bird/rodent, bird/opossum, bird/dog, bird/human, and horse/dog cross-reactions were the most common. Conclusions These results demonstrate a tendency in the eclectic feeding behavior of L. intermedia and support its potential role as a vector for ACL in the study area. .


Assuntos
Animais , Gatos , Bovinos , Cães , Feminino , Humanos , Insetos Vetores/fisiologia , Psychodidae/fisiologia , Aves , Brasil , Comportamento Alimentar/fisiologia , Cavalos , Insetos Vetores/classificação , Leishmaniose Cutânea/transmissão , Gambás , Testes de Precipitina , Psychodidae/classificação , Roedores
15.
Clin Microbiol Infect ; 19(4): E197-204, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23331929

RESUMO

Detection of Aspergillus IgG antibodies is important in the diagnosis of chronic pulmonary aspergillosis and allergic bronchopulmonary aspergillosis. Immunoprecipitation techniques to detect these antibodies appear to lack sensitivity and accurate quantitation compared with enzyme immunoassays (EIA). This study assessed the performance of two commercial EIAs compared with counterimmunoelectrophoresis (CIE). This was a prospective cohort study of 175 adult patients with chronic or allergic pulmonary aspergillosis. Aspergillus IgG antibodies were detected using CIE, Phadia ImmunoCap Aspergillus IgG and Bio-Rad Platelia Aspergillus IgG. Inter-assay reproducibility was determined for each method and 25 patients had two serum samples analysed within a 6-month interval. When compared with CIE, both ImmunoCap and Platelia Aspergillus IgG had good sensitivity (97 and 93%, respectively) for detection of Aspergillus IgG antibodies. The level of agreement between the two EIAs for positive results was good, but the concentration of antibodies was not correlated between the tests or with CIE titre. ImmunoCap IgG inter-assay coefficient of variation was 5%, whereas Platelia IgG was 33%. Median ImmunoCap IgG values for CPA and allergic aspergillosis were 95 and 32 mg/L, respectively, whereas Platelia IgG values were >80 and 6 AU/mL. The direction of CIE titre change over 6 months was mirrored by ImmunoCap IgG levels in 92% of patients, and by Platelia IgG in 72% of patients. Both ImmunoCap and Platelia Aspergillus IgG EIAs are sensitive measures of Aspergillus IgG antibodies compared with CIE. However, ImmunoCap appears to have better reproducibility and may be more suitable for monitoring patient disease.


Assuntos
Anticorpos Antifúngicos/sangue , Aspergilose Broncopulmonar Alérgica/diagnóstico , Aspergillus/imunologia , Técnicas Imunoenzimáticas/métodos , Testes de Precipitina/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Doença Crônica , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Adulto Jovem
16.
J Mycol Med ; 22(1): 72-82, 2012 Mar.
Artigo em Francês | MEDLINE | ID: mdl-23177817

RESUMO

Anti-Aspergillus antibody detection has been performed for over 50 years for the diagnosis of different chronic Aspergillus infections, starting with aspergilloma and later with chronic necrotizing pulmonary aspergillosis. It also enters into definition criteria for allergic broncho-pulmonary aspergillosis and contributes to the initial diagnosis of the aspergillosis, to the follow-up under treatment or to the detection of exacerbations. For the acute invasive aspergillosis, antibody detection has low interest compared to galactomannan antigen detection. Serology results have to be interpreted together with other clinical, radiological and biological, mycological criteria. This review describes the origins, the technical evolutions and the current place of Aspergillus serology in France. Finally, future improvements are discussed.


Assuntos
Anticorpos Antifúngicos/sangue , Antígenos de Fungos/imunologia , Aspergilose/sangue , Aspergilose/diagnóstico , Aspergillus/imunologia , França , Humanos , Pneumopatias Fúngicas/diagnóstico , Pneumopatias Fúngicas/imunologia , Testes de Precipitina/métodos , Testes de Precipitina/tendências , Testes Sorológicos/métodos , Testes Sorológicos/tendências
17.
Neth J Med ; 70(3): 136-8, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22516578

RESUMO

In this report a case of macro-aspartate aminotransferase in a 34-year-old pregnant woman is presented. Awareness of the existence of a macroenzyme is important because of their ability to cause diagnostic confusion, which leads to unnecessary investigations. Confirmation with a polyethylene glycol precipitation test is simple to perform and not expensive.


Assuntos
Aspartato Aminotransferases/sangue , Adulto , Feminino , Humanos , Peso Molecular , Polietilenoglicóis , Testes de Precipitina , Gravidez
18.
Med Mycol ; 50(6): 654-7, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22329454

RESUMO

Hypersensitivity pneumonitis (HP) is a pulmonary granulomatosis involving an immunoallergic mechanism caused by chronic inhalation of antigens, most frequently organic substances, as well as chemicals. We report the first European case of hypersensitivity pneumonitis due to the inhalation of Shiitake mushroom spores. A 37-year-old French Caucasian man with a one-month history of persistent dry cough, shortness of breath and loss of weight was admitted to our hospital on December 2010. Anamnesis showed he was involved in mushroom production beginning in the summer of 2010. His temperature on admission was 36.6°C and he had a normal blood pressure (135/90 mmHg). Bilateral fine crackles were audible in the base of both lungs. Pulmonary function tests showed a mild restrictive pattern with decreased DLco and a PaO(2) of 65 mmHg, Chest CT scan revealed reticulo-nodular shadows, slight ground glass opacities, liner atelectasis, and subpleural opacities in both lung fields. Bronchoscopy was normal but cytological examination of BAL revealed a predominant lymphocytosis (55%). Serum precipitins to the Shiitake mushroom spores were positive (3 precipitins arcs with high intensity) and as a result we advised the patient to cease his mushroom production activities. The diagnosis of hypersensitivity pneumonitis due to inhalation of Shiitake mushroom spores was established as a result of the improvement of all of his clinical symptoms, i.e., cough, weight loss, bilateral fine crackles, mild restrictive pattern of pulmonary function, and reticulo-nodular shadows on chest CT, once exposure was eliminated. Recent interest in exotic mushrooms varieties, e.g., Shiitake, in developed countries because of their possible medicinal properties might increase the potential risk of HP among mushrooms workers. Therefore, healthcare professionals have to take this new potential respiratory disease into account.


Assuntos
Alveolite Alérgica Extrínseca/microbiologia , Exposição por Inalação/efeitos adversos , Cogumelos Shiitake/imunologia , Esporos Fúngicos/imunologia , Adulto , Alveolite Alérgica Extrínseca/imunologia , Antígenos de Fungos/efeitos adversos , Antígenos de Fungos/imunologia , Líquido da Lavagem Broncoalveolar/imunologia , Broncoscopia , Humanos , Pulmão/microbiologia , Pulmão/patologia , Linfocitose/imunologia , Linfocitose/microbiologia , Masculino , Doenças Profissionais/imunologia , Doenças Profissionais/microbiologia , Testes de Precipitina , Precipitinas/sangue , Testes de Função Respiratória , Tórax/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Ultrassonografia
19.
Respir Med ; 105(8): 1238-47, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21514811

RESUMO

OBJECTIVES: Antisynthetase syndrome (ASS) is characterized by autoantibodies to aminoacyl-tRNA synthetases (anti-synthetase) and it is frequently associated with interstitial lung disease. The purpose of this study was to elucidate the prevalence and characteristics of the anti-synthetase positive subpopulation among idiopathic interstitial pneumonias (IIPs) and to clarify the importance of screening for these antibodies. METHODS: A retrospective study was performed in 198 consecutive cases with IIPs. Screening for six anti-synthetase antibodies was performed in all cases. Clinical profiles of all cases were compared with reference to the presence of anti-synthetase. High-resolution computed tomography (HRCT) findings of anti-synthetase positive cases were also analyzed. RESULTS: 13 cases (6.6%) were positive for anti-synthetase. Anti-EJ was most prevalent, followed by anti-PL-12. Onset ages of anti-synthetase positive cases were younger than those of anti-synthetase negative cases. Extrapulmonary features of ASS were absent in 6 anti-synthetase positive cases (46.2%). Histologically, among 5 UIP with lymphoid follicles and 11 NSIP cases, the prevalence of anti-synthetase positive cases was 8/16 (50%). On HRCT, ground glass opacity and traction bronchiectasis were the major findings in anti-synthetase positive cases, while honeycombing was absent. CONCLUSIONS: Anti-synthetase positive cases were not rare among IIPs. Anti-synthetase should be screened for in IIPs, especially in pathological NSIP or UIP with lymphoid follicles. These patients should be screened for anti-synthetase even if no suggestive extrapulmonary manifestation exists.


Assuntos
Aminoacil-tRNA Sintetases/imunologia , Autoanticorpos/análise , Doenças Autoimunes/diagnóstico , Doenças Pulmonares Intersticiais/imunologia , Miosite/diagnóstico , Adulto , Doenças Autoimunes/imunologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Miosite/epidemiologia , Miosite/imunologia , Testes de Precipitina/métodos , RNA de Transferência/metabolismo , Estudos Retrospectivos
20.
Rev Sci Tech ; 30(3): 821-30, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22435194

RESUMO

An outbreak of lumpy skin disease (LSD) occurred among cattle and water buffaloes in Egypt in 2006. Polymerase chain reaction (PCR) and the agar gel precipitation test (AGPT) were compared. Eight of ten (80%) tissue specimens from diseased cattle were positive with AGPT while 100% were positive with PCR. Of ten tissue specimens from diseased water buffaloes, 70% were positive with AGPT while 100% were positive with PCR. Ten milk samples were obtained from diseased water buffaloes; PCR detected nucleic acid of LSD virus (LSDV) in 50% while AGPT failed to detect LSDV antigen. Water buffaloes are susceptible to LSDV infection. The clinical signs of LSD were less severe in water buffaloes, but the virus was excreted in their milk. Diagnosis of LSD outbreaks by PCR will facilitate rapid application of control measures. Mass vaccination should be applied in both cattle and water buffaloes in Egypt using an effective specific vaccine against LSD, such as the attenuated Neethling strain vaccine or a recombinant vaccine.


Assuntos
Búfalos , DNA Viral/isolamento & purificação , Surtos de Doenças/veterinária , Doença Nodular Cutânea/diagnóstico , Vírus da Doença Nodular Cutânea/genética , Reação em Cadeia da Polimerase/veterinária , Animais , Bovinos , Embrião de Galinha , Egito/epidemiologia , Feminino , Doença Nodular Cutânea/epidemiologia , Doença Nodular Cutânea/virologia , Vírus da Doença Nodular Cutânea/isolamento & purificação , Leite/virologia , Reação em Cadeia da Polimerase/normas , Testes de Precipitina/métodos , Testes de Precipitina/veterinária
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