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1.
J Agric Food Chem ; 68(7): 2091-2098, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-31927882

RESUMO

In this study, we used reversed-phase high performance liquid chromatography (LC) to isolate three novel peptides with calcium-chelating capacity from tilapia bone collagen hydrolysate. Using LC-tandem mass spectrometry, we determined the amino acid sequences to be GPAGPHGPVG, FDHIVY, and YQEPVIAPKL. We then synthesized the three peptides and verified their calcium-chelating activity. Results showed that the calcium-chelating activity of GPAGPHGPVG, FDHIVY, and YQEPVIAPKL reached 18.80 ± 0.49, 35.73 ± 0.74, and 28.4 ± 0.94 mg/g, respectively. We next investigated how each peptide enhanced intestinal calcium absorption using Caco-2 cell monolayers. Compared with the control group, GPAGPHGPVG, FDHIVY, and YQEPVIAPKL potently enhanced calcium transport within 30 min by 89 ± 9, 202 ± 12, and 130 ± 7%, respectively. Results suggest that these peptides isolated from tilapia bone hydrolysate can be used as dietary supplements to increase calcium absorption.


Assuntos
Osso e Ossos/química , Cálcio/química , Cálcio/metabolismo , Quelantes/química , Colágeno/química , Proteínas de Peixes/química , Mucosa Intestinal/metabolismo , Peptídeos/química , Sequência de Aminoácidos , Animais , Transporte Biológico/efeitos dos fármacos , Células CACO-2 , Humanos , Mapeamento de Peptídeos , Hidrolisados de Proteína/química , Tilápia
2.
Chemosphere ; 238: 124586, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31442775

RESUMO

Cyantraniliprole can effectively control lepidopteran pests and has been used all over the world. In general, the risk of cyantraniliprole seems low for fish, but the toxicity selectivity among different fish species was not clear. Here the acute toxicity and chronic effects of cyantraniliprole to juvenile tilapia (Oreochromis mossambicus) were assessed. The results showed that 96 h LC50 of cyantraniliprole to tilapia was 38.0 mg/L. After exposed for 28 days, specific growth rates of the blank control, solution control, and the treatments of 0.037, 0.37 and 3.7 mg/L of cyantraniliprole were 1.14, 0.95, 0.93, 0.82 and 0.70% per day, respectively. The results of micronucleus experiment and single cell gel electrophoresis showed that cyantraniliprole damaged DNA in liver cells of tilapia larvae. Quantitative PCR results showed that cyantraniliprole could induce the up-regulation of Rpa 3 that is responsible for the DNA repair. The significantly down-regulation of Chk 2 gene was related to p53 pathway. It is therefore proposed that cyantraniliprole causes DNA damage in liver cells of tilapia and activates DNA damage and repair pathways.


Assuntos
Dano ao DNA/efeitos dos fármacos , Inseticidas/toxicidade , Fígado/patologia , Pirazóis/toxicidade , Tilápia/embriologia , Tilápia/crescimento & desenvolvimento , ortoaminobenzoatos/toxicidade , Animais , Quinase do Ponto de Checagem 2/biossíntese , Dano ao DNA/genética , Reparo do DNA/efeitos dos fármacos , Reparo do DNA/genética , Proteínas de Ligação a DNA/biossíntese , Brânquias/metabolismo , Hepatócitos/efeitos dos fármacos , Larva , Dose Letal Mediana , Alimentos Marinhos
3.
Artigo em Inglês | MEDLINE | ID: mdl-31868083

RESUMO

The Loskop Dam in South Africa is the most contaminated waterbody in the Olifants River which is a transboundary river that flows into Mozambique. The present study measured selected metal concentrations in the muscle of Labeo rosae and Oreochromis mossambicus from Loskop Dam, and assessed the human health risks associated with consumption of these fish species. Trace metals were below detection level in the surface water whereas bottom sediment exhibited relatively higher concentrations. A significant seasonal variations (p < 0.05) as well as inter-species difference (p < 0.05) of metal concentrations were observed in the fish muscle. Selenium concentration showed to have increase over the recent few years. Concentrations exceeding permissible level for human consumption was recorded for As, Se and Sb in both species. Other metals which THQ > 1 was Cr for L. rosae and Co for O. mossambicus. The current study shows that there could be some serious health and environmental implications for rural communities making use of Loskop Dam fish as food source. These findings add to knowledge in Africa, particularly South Africa, where other fish species have been identified as being potentially dangerous for human consumption in terms of Se, Sb, As, Co and Cr levels.


Assuntos
Minas de Carvão , Monitoramento Ambiental/métodos , Metalurgia , Metais/análise , Tilápia/metabolismo , Poluentes Químicos da Água/análise , Ácidos/análise , Animais , Humanos , Músculos/química , Rios/química , Alimentos Marinhos , África do Sul , Oligoelementos/análise
4.
Biosci. j. (Online) ; 35(6): 1915-1922, nov./dec. 2019. tab
Artigo em Inglês | LILACS | ID: biblio-1049163

RESUMO

The objective of this study was to evaluate the influence of the photoperiod on the physiological, hematological and reproductive parameters of tilapia males of the GIFT lineage. The treatments differed in the simulations of emeral photoperiods T1 = 0L: 24E T2 = 24L: 0E, T3 = 12L: 12E. Each aquarium was considered an experimental unit. In the present study, tilapia males under different photoperiods did not present significant differences in gonad weight and gonadosomatic index. At the end of the experiment, there were also no significant differences in hepatosomatic indexes (IHS) of Tilapia males under different treatments. In the present experiment, it was observed that the 0L: 24E treatment obtained a shorter testicular length in relation to the other treatments. In addition, the Tilapia submitted to 24L: 0E presented significant difference for the glucose levels, and did not present significant difference for cortisol levels and survival rate compared withthe other treatments. There were no statistical differences for hematological values. Exposure to different light regimes has shown that for GIFT tilapia, the photoperiod that promotes better reproductive index and well-being conditions is the photoperiod (12L: 12E).


O objetivo deste estudo foi avaliar a influência do fotoperíodo nos parâmetros fisiológicos, hematológicos e reprodutivos de machos de tilápia da linhagem GIFT. Os tratamentos diferiram nas simulações dos fotoperíodos emeral T1 = 0L: 24E T2 = 24L: 0E, T3 = 12L: 12E. Cada aquário foi considerado uma unidade experimental. No presente estudo, os machos de tilápia sob diferentes fotoperíodos não apresentaram diferenças significativas no peso gonadal e no índice gonadossomático. Ao final do experimento, também não houve diferenças significativas nos índices hepatossomáticos (IHS) dos machos de tilápias sob diferentes tratamentos. No presente experimento, observou-se que o tratamento 0L: 24E obteve menor comprimento testicular em relação aos demais tratamentos. Além disso, a tilápia submetida a 24L: 0E apresentou diferença significativa para os níveis de glicose, e não apresentou diferença significativa para os níveis de cortisol e sobrevivência em comparação com os demais tratamentos. Não houve diferenças estatísticas para valores hematológicos. A exposição a diferentes regimes de luz mostrou que, para a tilápia GIFT, o fotoperíodo que promove melhor índice reprodutivo e condições de bem-estar é o fotoperíodo (12L: 12E).


Assuntos
Tilápia , Fotoperíodo , Hidrocortisona
5.
Aquat Toxicol ; 217: 105336, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31733503

RESUMO

It is widely recognized that endocrine disrupting chemicals (EDCs) released into the environment through anthropogenic activities can have short-term impacts on physiological and behavioral processes and/or sustained or delayed long-term developmental effects on aquatic organisms. While numerous studies have characterized the effects of EDCs on temperate fishes, less is known on the effects of EDCs on the growth and reproductive physiology of tropical species. To determine the long-term effects of early-life exposure to common estrogenic chemicals, we exposed Mozambique tilapia (Oreochromis mossambicus) yolk-sac fry to 17ß-estradiol (E2) and nonylphenol (NP) and subsequently characterized the expression of genes involved in growth and reproduction in adults. Fry were exposed to waterborne E2 (0.1 and 1 µg/L) and NP (10 and 100 µg/L) for 21 days. After the exposure period, juveniles were reared for an additional 112 days until males were sampled. Gonadosomatic index was elevated in fish exposed to E2 (0.1 µg/L) while hepatosomatic index was decreased by exposure to NP (100 µg/L). Exposure to E2 (0.1 µg/L) induced hepatic growth hormone receptor (ghr) mRNA expression. The high concentration of E2 (1 µg/L), and both concentrations of NP, increased hepatic insulin-like growth-factor 1 (igf1) expression; E2 and NP did not affect hepatic igf2 and pituitary growth hormone (gh) levels. Both E2 (1 µg/L) and NP (10 µg/L) induced hepatic igf binding protein 1b (igfbp1b) levels while only NP (100 µg/L) induced hepatic igfbp2b levels. By contrast, hepatic igfbp6b was reduced in fish exposed to E2 (1 µg/L). There were no effects of E2 or NP on hepatic igfbp4 and igfbp5a expression. Although the expression of three vitellogenin transcripts was not affected, E2 and NP stimulated hepatic estrogen receptor (erα and erß) mRNA expression. We conclude that tilapia exposed to E2 and NP as yolk-sac fry exhibit subsequent changes in the endocrine systems that control growth and reproduction during later life stages.


Assuntos
Estradiol/toxicidade , Hormônio do Crescimento/metabolismo , Fenóis/toxicidade , Receptores Estrogênicos/metabolismo , Somatomedinas/metabolismo , Tilápia/crescimento & desenvolvimento , Poluentes Químicos da Água/toxicidade , Animais , Embrião não Mamífero/efeitos dos fármacos , Embrião não Mamífero/metabolismo , Fígado/efeitos dos fármacos , Masculino , Reprodução/efeitos dos fármacos , Tilápia/metabolismo , Vitelogeninas/metabolismo
6.
Ecotoxicol Environ Saf ; 186: 109752, 2019 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-31605954

RESUMO

Tilapias are cultured globally and are rising in acceptance as the most important freshwater aquaculture species. Monitoring of serum biomarkers is a promising tool in aquaculture to screen the health status as they are virtuous indicators of extreme stress and organ dysfunction in fish. The present study examined the serum biomarkers of oxytetracycline (OTC)-dosed Nile tilapia Oreochromis niloticus at 0, 80 and 800 mg/kg biomass/day, i.e., 0X, 1X, and 10X the approved dose (X = 80 mg OTC/kg biomass/day) for 10 consecutive days. The fish biomass and levels of serum glucose, aspartate aminotransferase, alanine aminotransferase, creatinine and C-reactive protein (CRP) were determined at scheduled intervals. A significant dose-dependent reduction in fish biomass during the OTC-dosing (5.84%) and post-OTC dosing (8.16%) periods was observed. All the serum biomarkers of Nile tilapia increased significantly on day 10 OTC-dosing. Though their levels reduced significantly, normalcy was not achieved even after 42 days of cessation of OTC-dosing, except CRP. The CRP reached the normal level on day 25 post-OTC dosing in the 1X group. The results, thus, demonstrated that the oral OTC-dosing influences the physiological state of apparently healthy Nile tilapia in a dose-dependent manner. These changes were, however, reversible upon discontinuation of OTC-dosing. The set of data observed on growth reduction and elevated serum biomarker levels even after 42 days of cessation of OTC-dosing, thus, raises questions on the utility of oral OTC-dosing.


Assuntos
Ração Animal , Antibacterianos/efeitos adversos , Aquicultura/métodos , Ciclídeos/fisiologia , Dieta , Oxitetraciclina/efeitos adversos , Estresse Fisiológico/efeitos dos fármacos , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Biomarcadores/sangue , Biomassa , Glicemia/metabolismo , Proteína C-Reativa/metabolismo , Ciclídeos/sangue , Ciclídeos/crescimento & desenvolvimento , Creatina/sangue , Humanos , Índia , Alimentos Marinhos , Tilápia
7.
Arq. bras. med. vet. zootec. (Online) ; 71(5): 1609-1615, set.-out. 2019. tab
Artigo em Português | LILACS, VETINDEX | ID: biblio-1038678

RESUMO

Objetivou-se avaliar a ocorrência de Aeromonas spp. em peixes e amostras de água na região semiárida de Pernambuco e avaliar a frequência de aerolissina (aerA), enterotoxina citotóxica (act), enterotoxina citotônica (alt) e serina protease (ahp) nesses isolados. Foram analisados 70 peixes vivos e oito mortos com sinais clínicos de aeromoniose e 16 amostras de água. Aeromonas spp. foram identificadas por análises microbiológicas (provas bioquímicas) e molecular, usando-se primers específicos para a região 16S rRNA, e a distribuição dos quatro fatores de virulência (aerA, alt, act e ahp) foi investigada por ensaio de PCR. Cento e cinquenta e cinco (84,7%) isolados foram confirmados como Aeromonas spp. na análise molecular. Os genes de virulência mais frequentes foram act (53,55%) e aerA (51,61%). De acordo com o tipo de amostra, observou-se maior frequência do gene aerA (87,5% P=0,0474) em isolados de peixes mortos e a menor frequência do gene act (47,73% P=0,0002) em peixes vivos. Este estudo demonstrou a presença de aeromoniose no cultivo de tilápias em tanques-rede, nos municípios de Jatobá e Petrolândia, na região semiárida de Pernambuco. A detecção de aerA, act e alt pode ser utilizada na tipagem de virulência de Aeromonas spp.(AU)


The purpose of this study was to evaluate the occurrence of Aeromonas spp. from fishes and tilapia net-cage farm water in semi-arid regions of Pernambuco and to evaluate the frequency of the aerolysin (aerA), cytotoxic enterotoxin (act), cytotonic enterotoxin (alt) and serine protease (ahp) genes in Aeromonas isolates. 70 live and eight dead fish with aeromoniosis clinical signs and 16 water samples were analyzed. Aeromonas spp. isolated were identified by microbiological (biochemical evidence) and molecular analysis using specific primers for 16SrRNA region, while the distribution of four virulence factors, including aerA, alt, act and ahp, was investigated by PCR assay. One hundred fifty-five (84.7%) isolates were confirmed as Aeromonas spp. by molecular analysis. The most frequent virulence genes in isolates were act (53.55%) and aerA (51,61%). According to the kind of sample, the higher frequency of aerA gene (87.5% P= 0.0474) was observed in isolates from dead fish and the lowest frequency of act gene (47.73% P= 0.0002) from live fish. This study found the presence of aeromoniosis on tilapia farming in net-cages on Jatobá and Petrolândia counties in the semiarid Pernambuco region. The detection of aerA, act and alt can be used for virulence typing of Aeromonas spp. isolates.(AU)


Assuntos
Animais , Tilápia/microbiologia , Aeromonas/patogenicidade , Ciclídeos/microbiologia , Pesqueiros , Virulência
8.
Fish Shellfish Immunol ; 94: 566-579, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31550509

RESUMO

The changes in the ovarian stroma of the fish during their spawning season become it an excellent biological model for studies on cellular and vascular elements due to the intense tissue remodeling in fish occur naturally throughout this critical period. The present study aims to investigate the cellular and vascular components of the ovarian stroma of Redbelly tilapia during the spawning season by conventional, immunohistochemical stains as well as to detect the ultrastructural characteristics for each stromal component. The histological examinations revealed a series of blood vessels with special structures, include throttle artery, glomus, spirally oriented arterioles, modified arteries, and veins as well as arteriovenous anastomosis. Various types of cells were detected in the stroma include; telocytes, rodlet cells, mast cells, eosinophils, neutrophils, lymphocytes, fibroblasts, macrophages, melanocytes, adipocytes, dendritic cells, and endocrine (steroidogenic, interstitial) cells. Moreover, these stromal cells showed a broad range of staining affinity against c-kit, desmin, and s100-protein. Bundles of nerve fibers were detected between the follicles. This study exposed various cellular and vascular components with distinct functions in the ovary of Redbelly tilapia during the spawning season.


Assuntos
Ovário/fisiologia , Células Estromais/fisiologia , Tilápia/fisiologia , Animais , Feminino , Histocitoquímica/veterinária , Imuno-Histoquímica/veterinária , Microscopia Eletrônica de Transmissão/veterinária , Ovário/citologia , Ovário/ultraestrutura , Reprodução , Estações do Ano , Células Estromais/citologia , Células Estromais/ultraestrutura
9.
Fish Shellfish Immunol ; 94: 697-704, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31561027

RESUMO

The employment of traditional bait and medicament in freshwater aquaculture causes the frequent occurrence of environmental pollution and disease. Effluent collected after Rhodopseudomonas capsulatus-mediated wastewater treatment could be re-utilized as microbial feeds, medicament, and aquaculture water to culture Tilapia mossambica. Therefore, a novel integrated system of wastewater treatment using effluent containing R. capsulatus that improves yield, increases disease resistance, and enhances the quality of aquaculture water for Tilapia mossambica culture was proposed and investigated. Tilapia mossambica can grow well in effluent containing R. capsulatus (ER). The survival rate, yield, and whole body composition of the ER group were all increased compared to the control group (CK). The biochemical (B vitamin) and other substances in the effluent of R. capsulatus enhanced the activity of AKP, ACP, phagocytic, SOD and CAT by upregulating the expression of AKP, ACP, SOD and CAT genes. Theoretical analysis showed that biochemical molecules regulate the expression of these gene and enzyme activities by acting as a signal that stimulates the active center. Moreover, biochemical molecules present in R. capsulatus enhanced the activity of the mTOR and NF-kB signaling pathways. Furthermore, R. capsulatus inhibited Aeromonas hydrophila that increases resistance against fish disease. Meanwhile, R. capsulatus in the effluent also improved the aquaculture water quality. This technology would save the aquaculture water, reduce water pollution and wastewater discharge, and increase the output and disease resistance of Tilapia mossambica, simultaneously.


Assuntos
Aquicultura/métodos , Doenças dos Peixes/imunologia , Rhodobacter capsulatus/química , Tilápia/imunologia , Eliminação de Resíduos Líquidos/métodos , Aeromonas hydrophila/fisiologia , Animais , Resistência à Doença/genética , Regulação da Expressão Gênica/imunologia , Infecções por Bactérias Gram-Negativas/imunologia , Infecções por Bactérias Gram-Negativas/veterinária , NF-kappa B/genética , Distribuição Aleatória , Transdução de Sinais/imunologia , Serina-Treonina Quinases TOR/genética , Águas Residuárias/microbiologia
10.
Artigo em Inglês | MEDLINE | ID: mdl-31473330

RESUMO

Estradiol (E2) is a sex steroid hormone that modulates multiple physiological processes in teleosts. The aim of this study was to explore the role of E2 in the hepatic lipid metabolism of hybrid tilapia. The hybrid tilapias were injected with different concentrations of E2 (0 mg/kg, 10 mg/kg, 25 mg/kg and 50 mg/kg) and ICI 182,780 (ICI) (35 mg/kg) (an E2 receptor antagonist). Subsequently, the liver lipid depositions were analyzed by tissue sections with oil red O staining. Serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and nonesterified fatty acids (NEFAs) were assayed from the fish in different groups. Genes related to very low-density lipoprotein (VLDL) assembly, lipoprotein lipase and lipoprotein receptors, fatty acid uptake and triacylglycerol metabolism were determined by quantitative RT-PCR. The results showed that 50 mg/kg E2 injections enlarged the lipid droplets significantly. Simultaneously, the E2 injections tended to upregulate TC, TG, LDL, and HDL in the serum. The 50 mg/kg E2 group showed a significantly higher expression of the VLDL assembly genes but depressed levels of LDLR and LRP1. In addition, FABP3, FABP11a and DGAT2 were significantly elevated, while CD36 and ACO1 decreased in the 50 mg/kg E2 injection. The ICI injection inhibited the expression of MTP, LPL, LRP1, CD36, FABP11a, ACO1 and FAS in tilapia livers. These results demonstrated that by stimulating the expression of genes associated with the VLDL assembly, inhibiting lipoprotein lipase and lipoprotein receptor-related genes and promoting the rate-limiting enzyme in the synthesis of the TG, E2 induced deposition of lipids in the livers of hybrid tilapia. Overall, the results suggest a role for E2 in fish lipid metabolisms that provide new clues to illustrate the sex steroid function in energy metabolism in livers.


Assuntos
Estradiol/farmacologia , Proteínas de Peixes/biossíntese , Regulação da Expressão Gênica/efeitos dos fármacos , Fígado/metabolismo , Tilápia/metabolismo , Triglicerídeos/biossíntese , Animais , Cruzamentos Genéticos , Relação Dose-Resposta a Droga , Feminino , Proteínas de Peixes/genética , Masculino , Tilápia/genética , Triglicerídeos/genética
11.
Syst Parasitol ; 96(8): 715-722, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31515681

RESUMO

A new species of Dermoergasilus Ho & Do, 1982 (Copepoda: Ergasilidae) parasitic on the gills of the redbelly tilapia Coptodon zillii (Gervais) from Basrah, southern Iraq, is described. Dermoergasilus cichlidus n. sp. is morphologically similar to D. longiabdominalis El-Rashidy & Boxshall, 2001 in the body length to width ratio, the swimming legs armature, the shape of the urosome and the small slightly spiniform seta on the exopodal segment of leg 5. However, the main differences are the presence of long setae on the endopod of leg 1 in D. cichlidus n. sp. (vs relatively short setae); the inner apical spine on the tip of the endopod of leg 1 being only slightly longer than the outer apical spine (vs inner apical spine about twice as long as the outer); the first interpodal sternite ornamented with spinules (vs interpodal sternites not ornamented); and the caudal rami and anal somite of equal length (vs caudal rami length c.2/3 of the length of the anal somite). Dermoergasilus occidentalis can be distinguished from D. cichlidus n. sp. by the relative length of the antennal segments, the absence of a minute terminal spine on the digital process of the caudal ramus and by differences in the mouth parts.


Assuntos
Copépodes/classificação , Copépodes/fisiologia , Tilápia/parasitologia , Animais , Copépodes/anatomia & histologia , Brânquias/parasitologia , Iraque , Especificidade da Espécie
12.
Protein Pept Lett ; 26(12): 887-892, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31544688

RESUMO

BACKGROUND: Lectins have been studied in recent years due to their immunomodulatory activities. OBJECTIVE: We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes. METHODS: Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine incorporation, cytokines were investigated using ELISA assay and cell viability assay was performed by investigation of damage through signals of apoptosis and necrosis. RESULTS: OniL did not promote significant cell death, induced high mitogenic activity in relation to control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines. CONCLUSION: These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent in immunostimulatory assays.


Assuntos
Proliferação de Células/efeitos dos fármacos , Lectinas de Ligação a Manose/farmacologia , Mitógenos/farmacologia , Baço/citologia , Animais , Morte Celular , Linhagem Celular , Sobrevivência Celular , Concanavalina A/farmacologia , Citocinas/biossíntese , Masculino , Camundongos Endogâmicos BALB C , Tilápia
13.
Environ Pollut ; 255(Pt 1): 113039, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31521994

RESUMO

Although the poultry production sector plays a key role in sustaining the majority of animal protein demand in Egypt, the deleterious effects of widespread antibiotic resistance on health and environment are currently not well recognized. Litter and dropping samples from broiler and layer poultry farms as well as, tilapia samples from the Nile River and aquaculture farms were collected from Upper Egypt. Samples were extracted and examined for tetracycline residues [tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and doxycycline (DC)] using HPLC. In addition, tetracycline resistance genes [tet (M), tet (W), tet (Q) and tet (G)] were screened from pooled intestinal contents collected from twelve broiler farms in Upper Egypt. The antibiotic resistance genes results revealed that tet (W) was confirmed to be expressed in all intestinal samples. In contrast, tet (Q) and tet (M) were detected only in 42% and 17% of the samples, respectively. CTC and OTC were the antimicrobial compounds with the highest concentrations in poultry litter and droppings, with concentrations of 6.05 and 2.47 µg g-1 (CTC) and 5.9 and 1.33 µg g-1 (OTC), respectively. However, the concentrations of DC were significantly higher than those of the other compounds in both aquaculture and Nile River tilapia. The tetracycline residue levels in aquaculture tilapia were significantly higher than those in Nile River tilapia. The hazard quotients (HQs) exceeded 1 for OTC, CTC and DC, which highlights the great risk of using broiler litter to fertilize agricultural land. Moreover, the presence of DC and CTC indicates that consumption of aquaculture tilapia poses a considerable health risk. Therefore, poultry litter or droppings containing tetracycline residues and tet resistance determinants used for aquaculture or as farmland fertilizers could be major sources of antibiotic resistance in fish, humans and environment.


Assuntos
Antibacterianos/análise , Galinhas/crescimento & desenvolvimento , Resistência a Tetraciclina/genética , Tetraciclina/análise , Tilápia/crescimento & desenvolvimento , Drogas Veterinárias/análise , Animais , Aquicultura , Ecologia , Egito , Fazendas , Fertilizantes , Conteúdo Gastrointestinal/química , Conteúdo Gastrointestinal/microbiologia , Esterco/análise , Esterco/microbiologia , Músculos/química , Aves Domésticas , Medição de Risco , Alimentos Marinhos/análise
14.
J Fish Dis ; 42(11): 1543-1552, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31515819

RESUMO

Tilapia lake virus (TiLV)-suspected samples of tilapia were collected from grow-out ponds located with clinical signs and mortality ranged from 5% to 50%. The reverse transcription-polymerase chain reaction (RT-PCR) assay revealed the presence of TiLV in the disease outbreak ponds. Cell lines were developed from heart, gill and eye of Mozambique tilapia and characterized. Morphologically, these cell lines are composed of epithelioid cells. The optimum growth of these cells was observed at 28°C and 20% concentration of FBS. After cryopreservation, 70%-90% of cells were found to be viable. The cells of all three cell lines were found to be positive to fibronectin and pancytokeratin. PCR amplification of 16S rRNA and COI of O. mossambicus confirmed the origin of these cell lines from O. mossambicus. Heart and gill cell lines were found to be highly susceptible to TiLV and found to be useful for its isolation from infected fish samples. The experimental infection was carried out in O. niloticus and O. mossambicus using the TiLV propagated in susceptible cell lines. The RT-PCR results revealed the presence of TiLV in brain, gill, liver, kidney, spleen, eye, muscle, intestine and heart of experimentally infected O. niloticus and O. mossambicus.


Assuntos
Suscetibilidade a Doenças/veterinária , Doenças dos Peixes/virologia , Infecções por Vírus de RNA/veterinária , Vírus de RNA/fisiologia , Tilápia , Animais , Linhagem Celular , Infecções por Vírus de RNA/virologia
15.
J Appl Genet ; 60(3-4): 393-400, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31473935

RESUMO

Red tilapia is a phenotype with potential value to the tilapia industry due to its attractive skin coloration. However, the presence of black blotches scattered on the body makes these fish less valuable than blemish-free red fish. The correlation between the degree of blotching and other economic traits is important for selective breeding programs including high-performance red fish without black blotches. Therefore, this study aimed to assess the magnitude and direct and indirect effects of blotching in crossbred red tilapia on the growth rate and body measure traits using path analysis methodology. Six genetic groups were evaluated in terms of body measurements, body weight rate, and proportion of black blotching over 250 days of growth: Chitralada and Red-Stirling; crossbred F1-♀ Red-Stirling × â™‚ Chitralada and ♀ Chitralada × â™‚ Red-Stirling, and the introgressive crossbreeding-♀ CR × â™‚ Chitralada and ♀ Chitralada × â™‚ CR (C × CR). All variables showed high and positive linear correlations. Individuals with black blotches had lower coefficients of correlation with other variables (body weight and measurements); the direct and indirect effects are near zero. The path analysis indicated a lack of genetic correlation between the degree of black blotches and the economic traits of red tilapia. This facilitates the development of high growth performance red fish with blotched-free reddish skin.


Assuntos
Peso Corporal/genética , Hibridização Genética/genética , Tilápia/genética , Animais , Aquicultura , Cruzamento , Fenótipo , Pigmentação/genética , Tilápia/crescimento & desenvolvimento
16.
Rev. bras. cir. plást ; 34(3): 349-354, jul.-sep. 2019. ilus
Artigo em Inglês, Português | LILACS | ID: biblio-1047152

RESUMO

Introdução: A produção e a distribuição de pele humana pelos Bancos de Pele do Brasil é insuficiente para atender a demanda do país, suprindo menos de 1% da necessidade para tratar as vítimas de queimaduras. O objetivo do trabalho foi apresentar a elaboração e o desenvolvimento da implantação do primeiro Banco de Pele Animal do Brasil para tratamento da queimadura. Métodos: Trata-se de um estudo metodológico. O Banco de Pele Animal Aquático foi elaborado, desenvolvido e instalado a partir do desenvolvimento do processamento de pele da tilápia, da revisão sistemática de estudos referentes a banco de pele animal, de visitas à pscicultura em Jaguaribara-CE, da visita técnica aos bancos de pele humana no Brasil, de consulta técnica e de treinamento no Banco de Pele de Recife, da observação de todas as fases de processamento da pele de tilápia e da identificação da estrutura física da área onde ocorrem todos os processos. Resultados: Além da produção e da distribuição da pele da tilápia para os estudos em vítima de queimaduras, o banco está processando mais de 5000 peles de tilápia e encontra-se em fase de distribuição desta pele para estudos multicêntricos em outros estados e outras especialidades, que desenvolvem pesquisas nas áreas de ginecologia, ortopedia, endoscopia, estomaterapia, cirurgia vascular, odontologia e veterinária. Conclusão: este trabalho possibilitou a elaboração, o desenvolvimento e a implementação do primeiro Banco de Pele Animal do país e o primeiro Banco de Pele Aquática do mundo.


Introduction: The production and distribution of human skin by Brazilian skin banks is insufficient to meet the country's demand, supplying less than 1% of the need for treating burn victims. The objective of this work was to present the elaboration and development of the first animal skin bank of Brazil for the treatment of burns. Methods: This methodological study elaborated, developed, and installed The Aquatic Animal Skin Bank in terms of the development of tilapia skin processing after a systematic review of studies referring to animal skin banks based on visits to the pisciculture center in Jaguaribara-CE, technical visits to human skin banks in Brazil, technical consultation and training in the Recife Skin Bank, the observation of all phases of tilapia skin processing, and the identification of the physical structure of the area where the processes occur. Results: In addition to the production and distribution of tilapia skin for studies on burn victims, the bank is processing more than 5000 tilapia skin samples and is in the distribution phase of this skin for multicentric studies in other states and specialties including gynecology, orthopedics, endoscopy, stomatherapy, vascular surgery, dentistry, and veterinary medicine. Conclusion: This work enabled the elaboration, development, and implementation of Brazil's first animal skin bank and the world's first aquatic skin bank.


Assuntos
Animais , História do Século XXI , Materiais Biocompatíveis , Materiais Biocompatíveis/uso terapêutico , Curativos Biológicos , Queimaduras , Tilápia , Pele Artificial , Ciclídeos , Pesqueiros , Materiais Biocompatíveis/análise , Curativos Biológicos/normas , Curativos Biológicos/provisão & distribução , Queimaduras/reabilitação , Tilápia/cirurgia , Pele Artificial/normas , Ciclídeos/cirurgia , Pesqueiros/normas
17.
Biomed Pharmacother ; 117: 109204, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31387177

RESUMO

We wished to investigate the role of a tilapia skin collagen polypeptide (TSCP; molecular weight <3 kDa) in alleviating liver and kidney injuries in aging mice induced by d-galactose (d-gal) and its underlying mechanism of action. First, we characterized TSCP. TSCP was passed through a 3-kDa ultrafiltration membrane, desalted in water by a solid-phase extraction column, purified further by reverse phase-high performance liquid chromatography, and analyzed by electrospray ionization mass spectrometry and tandem mass spectrometry. TSCP contained 17 types of amino acids (AAs) and 41 peptide chains of length 7 AAs to 22 AAs. The content of free AAs and total AAs of TSCP was 13.5% and 93.79%, respectively. Next, we undertook animal experiments. Mice were injected once-daily with D-gal (300 mg/kg body weight, s.c.) for 8 weeks, and TSCP was administered simultaneously once-daily by intragastric gavage. TSCP could visibly improve the decreased body weight, depressed appetite, and mental deterioration of mice triggered by d-gal. TSCP could also alleviate d-gal-induced damage to the liver and kidneys according to histopathology (especially high-dose TSCP). Consistent with these macroscopic and pathologic changes, TSCP could also prevent d-gal-induced increases in serum levels of alanine aminotransferase, aspartate transaminase, alkaline phosphatase, lipid peroxidation, creatinine and uric acid, as well as decreases in serum levels of immunoglobulin (Ig)G and IgM. Moreover, TSCP improved the activities of superoxide dismutase, catalase, and glutathione peroxidase, but also inhibited the increases in the levels of malondialdehyde and inducible nitric oxide synthase expression in the liver and kidneys of d-gal-treated mice. These results suggest that TSCP can alleviate the injuries to the liver and kidneys in aging mice induced by d-gal, and that its mechanism of action might be, at least partially, associated with attenuation of oxidative stress and enhancement of immune function.


Assuntos
Colágeno/farmacologia , Galactose/efeitos adversos , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Peptídeos/farmacologia , Substâncias Protetoras/farmacologia , Tilápia/metabolismo , Alanina Transaminase/metabolismo , Animais , Antioxidantes/metabolismo , Aspartato Aminotransferases/metabolismo , Catalase/metabolismo , Glutationa Peroxidase/metabolismo , Rim/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/metabolismo , Masculino , Malondialdeído/metabolismo , Camundongos , Estresse Oxidativo/efeitos dos fármacos , Superóxido Dismutase/metabolismo
18.
Environ Toxicol Pharmacol ; 71: 103222, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31426013

RESUMO

The Roodeplaat Dam and its three inflowing rivers are highly impacted by surrounding anthropogenic activities. The system is hyper-eutrophic and characterized by seasonal algal blooms and previous studies have reported levels of the hepatotoxin microcystin in the water of the impoundment. Limited information is available on the microcystin concentrations in the inflowing rivers and no information is available on the bioaccumulated levels and potential health effects in fish inhabiting these rivers. The aim of this study was to do a histopathological assessment and to determine the concentrations of bioaccumulated microcystins in the livers of two indicator fish species Clarias gariepinus and Oreochromis mossambicus. The results showed that the two species bioaccumulate microcystins at different concentrations and that their hepatic health response varied. The liver index was significantly higher for C. gariepinus compared to O. mossambicus. No significant positive correlation was found between the bioaccumulated microcystin levels and the liver histology index. It is recommended that this pilot study be followed by a controlled exposure study to confirm a possible cause and effect relationship between microcystin exposure and the specific liver alterations identified.


Assuntos
Peixes-Gato/metabolismo , Fígado/efeitos dos fármacos , Fígado/patologia , Microcistinas/toxicidade , Rios/química , Tilápia/metabolismo , Poluentes Químicos da Água/toxicidade , Animais , 32418 , Monitoramento Ambiental , Eutrofização , Fígado/metabolismo , Microcistinas/metabolismo , Projetos Piloto , África do Sul , Especificidade da Espécie , Poluentes Químicos da Água/metabolismo
19.
J Contemp Dent Pract ; 20(4): 403-404, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31308267

RESUMO

Tilapia hepcidin (TH), an antimicrobial peptide (AMP) derived from tilapia (Oreochromis mossambicus) forms an important component of the fish innate immune defense.1 However, the functionality of TH is not just limited to its antimicrobial actions, significant applications in cancer research remain an area to be explored. Antimicrobial peptides derived from fish and shrimp demonstrated anticancer activities in addition to their previously discovered antimicrobial action. Three hepcidin-like AMPs can be isolated from tilapia, namely TH1-5, TH2-2, and TH2-3. Of these, TH1-5 and TH2-3 have shown potent antitumor activity.2.


Assuntos
Neoplasias Bucais , Tilápia , Animais , Antibacterianos , Hepcidinas
20.
J Fish Dis ; 42(10): 1383-1389, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31322740

RESUMO

Tilapia lake virus (TiLV) is an emerging virus associated with high fish mortality and economic losses. This study investigates the virucidal effects of the following disinfectants (active ingredients) on TiLV: 2.5 ppm iodine, 10 ppm sodium hypochlorite (NaOCl), 300 ppm hydrogen peroxide (H2 O2 ), 80 ppm formalin and 5,000 ppm (0.5%) Virkon® . Factors that affect the disinfectants' efficacy, including temperature, contact time and soiling (organic matter) interference, were examined under conditions mimicking natural aquaculture practices. TiLV inactivation of higher than 5 log10 TCID50  ml-1 was achieved after 10 min and at 28°C for all disinfectants except formalin; similar inactivation levels were reached by NaOCl and Virkon® at 10 min and 4°C. Extended exposure to formalin from 10 to 60 min at 28°C rendered more than 5 log10 inactivation. Increasing synthetic organic matter in the water to mimic soiling interference reduced the efficacy of NaOCl, iodine and H2 O2 when tested at 10 min and 28°C; however, Virkon® still achieved more than 5 log10 inactivation. This study demonstrates that most common disinfectants effectively reduced viral loads to minimum levels. To limit the spread of TiLV in aquaculture farms and related facilities, the appropriate use of such disinfectants should therefore be promoted and implemented.


Assuntos
Desinfetantes/farmacologia , Doenças dos Peixes/tratamento farmacológico , Tilápia , Viroses/veterinária , Inativação de Vírus , Animais , Doenças dos Peixes/virologia , Especificidade da Espécie , Viroses/tratamento farmacológico , Viroses/virologia , Vírus/efeitos dos fármacos
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