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1.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(6): 2009-2013, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-31839075

RESUMO

OBJECTIVE: To investigate the infection rate of Toxoplasma gondii in patients with hematological diseases. METHODS: The Toxoplasma gondii IgM antibody in 200 patients with hematological diseases were tested, at the same time, IgM antibody in the persons received physical examination and other patients with common clinical diseases also were test, and their detection results were compared. RESULTS: The positive rate of Toxoplasma gondii IgM antibody in patients with hematological diseases was 7.50%, the positive rate in persons received physical examination was 0.67%, and the positive rate in patients with other common clinical diseases was 1.20%. The positive rate of IgM antibody in patients with hematological diseases was statistically significantly higher than that in the latter two kinds of persons(P<0.05). Among the patients with hematological diseases, the positive rate of Toxoplasma gondii IgM antibody in patients with bone marrow neoplastic diseases was 10.32%, which was statistically significantly higher than that in patients with bone marrow non-neoplastic diseases (2.70%). CONCLUSION: Patients with hematological diseases are susceptible to Toxoplasma gondii, and to whom enough attention should be paid.


Assuntos
Doenças Hematológicas , Toxoplasmose , Anticorpos Antiprotozoários , Doenças Hematológicas/complicações , Humanos , Imunoglobulina G , Imunoglobulina M , Toxoplasmose/complicações
2.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(4): 427-430, 2019 Sep 26.
Artigo em Chinês | MEDLINE | ID: mdl-31612681

RESUMO

OBJECTIVE: To investigate the prevalence of the serum anti-Toxoplasma gondii antibody among patients with malignant tumors of the digestive tract in Hainan Province. METHODS: A total of 1 932 patients with malignant tumors of the digestive tract were enrolled in Hainan Province from 2016 to 2019, including 376 esophageal cancer patients, 475 gastric cancer patients, 401 colorectal cancer patients, 427 hepatic cancer patients and 253 pancreatic cancer patients, and 400 healthy people served as controls. The serum IgG and IgM antibodies specific to T. gondii were detected using enzyme-linked immunosorbent assay, and the seroprevalence was compared. RESULTS: The overall seroprevalence of anti-T. gondii IgG antibody was significantly greater in patients with malignant tumors of the digestive tract than in healthy controls (19.82% vs. 3.75%; χ2 = 60.49, P < 0.01), and no significant difference was seen in the overall seroprevalence of anti-T. gondii IgM antibody between patients with malignant tumors of the digestive tract and healthy controls (1.09% vs. 0.50%; χ2 = 1.17, P > 0.05). The seroprevalence of anti-T. gondii IgG antibody was 15.16%, 19.58%, 21.70%, 23.65% and 17.79% in patients with esophageal cancer, gastric cancer, colorectal cancer, hepatic cancer and pancreatic cancer, which was all significantly greater than in healthy controls ( χ2 = 29.97, 50.29, 58.03, 67.85 and 36.59; all P < 0.01); however, the seroprevalence of anti-T. gondii IgG antibody in patients with esophageal cancer (1.06%), gastric cancer (1.47%), colorectal cancer (0.75%), hepatic cancer (1.17%) and pancreatic cancer (0.79%) did not differ from that in healthy controls ( χ2 = 0.80, 2.02, 0.20, 1.11 and 0.21; all P > 0.05). There was a significant difference in the seroprevalence of anti-T. gondii IgG antibody among various types of malignant tumors of the digestive tract ( χ2 = 10.65, P < 0.05); however, no significant difference was detected in the seroprevalence of anti-T. gondii IgM antibody ( χ2 = 1.33, P > 0.05). CONCLUSIONS: There is a high seroprevalence of anti-T. gondii IgG antibody among patients with malignant tumors of the digestive tract in Hainan Province, and there is a significant difference in the seroprevalence in terms of the cancer type. It is suggested that the screening for T. gondii infections should be intensified in patients with malignant tumors of the digestive tract to effective prevent and control the damages to patients with malignant tumors of the digestive tract caused by T. gondii infections.


Assuntos
Gastroenteropatias , Neoplasias , Toxoplasmose , Anticorpos Antiprotozoários/sangue , Gastroenteropatias/complicações , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Neoplasias/complicações , Estudos Soroepidemiológicos , Toxoplasma , Toxoplasmose/sangue , Toxoplasmose/complicações , Toxoplasmose/prevenção & controle
3.
Afr Health Sci ; 19(2): 1858-1865, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31656468

RESUMO

Background: A significant proportion of newborns in the developing countries are born with congenital anomalies. Objective: This study investigated congenital infections due to Rubella virus, Toxoplasma gondii, Treponema pallidum among presumed normal neonates from full term pregnant women in Mwanza, Tanzania. Methods: Sera from mothers were tested for Treponema pallidum and Toxoplasma gondii infection while newborns from mothers with acute infections were tested for T. pallidum and T. gondii, and all newborns were tested for Rubella IgM antibodies. Results: A total of 13/300 (4.3 %) mothers had T. pallidum antibodies with 3 of them having acute infection. Two (0.7 %) of the newborns from mothers with acute infection were confirmed to have congenital syphilis. Regarding toxoplasmosis, 92/300 (30.7 %) mothers were IgG seropositive and 7 had borderline positivity, with only 1/99 (1%) being IgM seropositive who delivered IgM seronegative neonate. Only 1/300 (0.3 %) newborn had rubella IgM antibodies indicating congenital rubella infection. Conclusion: Based on these results, it is estimated that in Mwanza city in every 100,000 live births about 300 and 600 newborns have congenital rubella and syphilis infections, respectively. Rubella virus and T. pallidum are likely to be among common causes of congenital infections in developing countries.


Assuntos
Complicações Infecciosas na Gravidez/epidemiologia , Vírus da Rubéola/imunologia , Rubéola (Sarampo Alemão)/congênito , Rubéola (Sarampo Alemão)/epidemiologia , Sífilis Congênita/epidemiologia , Toxoplasma/imunologia , Toxoplasmose Congênita/epidemiologia , Treponema pallidum/imunologia , Adolescente , Adulto , Anticorpos Antivirais/sangue , Estudos Transversais , Feminino , Sangue Fetal/imunologia , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Recém-Nascido , Gravidez , Complicações Infecciosas na Gravidez/imunologia , Cuidado Pré-Natal , Prevalência , Rubéola (Sarampo Alemão)/diagnóstico , Vírus da Rubéola/isolamento & purificação , Estudos Soroepidemiológicos , Sífilis/complicações , Sífilis/epidemiologia , Sífilis Congênita/diagnóstico , Tanzânia/epidemiologia , Toxoplasma/isolamento & purificação , Toxoplasmose/complicações , Toxoplasmose/epidemiologia , Toxoplasmose Congênita/diagnóstico , Treponema pallidum/isolamento & purificação , População Urbana
4.
Turkiye Parazitol Derg ; 43(Suppl 1): 1-7, 2019 10 07.
Artigo em Inglês | MEDLINE | ID: mdl-31587535

RESUMO

Objective: Since symptomatic toxoplasmosis in in human immunodeficiency virus (HIV)/Acquired immunodeficiency syndrome (AIDS) almost always occurs as a result of reactivation of chronic infection, screening Toxoplasma serology has an important role in the follow up of the main disease in these populations. In this meta-analysis, we aimed to reveal the difference in the seroprevalence rates of Toxoplasma gondii infection between groups in relation to CD4 counts (CD4-counts ≥200 and <200 cells/mm3) HIV-infected population. Methods: The meta-analysis was performed by searching for the studies in English that were published in the last 20-year period in databases including PubMed, Google Scholar, Embase, Science Direct and Web of Science. The process of searching was carried out using the keywords: "Acquired immunodeficiency syndrome", "AIDS", "Human immunodeficiency virus", "HIV", "Toxoplasma", "Toxoplasmosis", "Toxoplasma gondii", "seroprevalence", "prevalence" and "immunoglobulin G". Results: A total of 16 studies including 3982 seropositive samples of T. gondii, 2792 of which were in first group (HIV positive patients with CD4-counts ≥200 cells/mm3) and 1190 were in second group (HIV positive patients with CD4-counts <200 cells/mm3), were included in the meta-analysis. The seroprevalence of T. gondii was 40.03% in HIV-positive patients with CD4 counts ≥200 cells/mm3, and 43.5% in the group with CD4 counts <200 cells/mm3. Seroprevalence rates in the studies included in the meta-analysis showed variability (heterogeneity) in both groups and heterogeneity between studies was higher in group 1 [Group 1; Cochran Q=994.16, DF=15, I²=98.49%, p<0.0001 and group 2; Cochran Q=368.50, DF=15, I²=95.93%, p<0.0001]. Conclusion: We concluded that HIV/AIDS patients with low CD4 counts have higher epidemiological risk as well as immunological risk of toxoplasmosis. To the best of our knowledge, this is the first meta-analysis evaluating the seroprevalence of T. gondii in AIDS/HIV population by comparing the seroprevalance of T. gondii in subgroups formed according to CD4 counts.


Assuntos
Síndrome de Imunodeficiência Adquirida/complicações , Toxoplasma/imunologia , Toxoplasmose/epidemiologia , Síndrome de Imunodeficiência Adquirida/imunologia , Anticorpos Antiprotozoários/sangue , Contagem de Linfócito CD4 , Feminino , Infecções por HIV/complicações , Infecções por HIV/imunologia , Humanos , Imunoglobulina G/sangue , Masculino , Fatores de Risco , Estudos Soroepidemiológicos , Toxoplasmose/complicações , Toxoplasmose/imunologia
5.
BMC Infect Dis ; 19(1): 826, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31533667

RESUMO

BACKGROUND: The association between Toxoplasma gondii infection and thyroid disease has been poorly studied. Therefore, we sought to determine the association between T. gondii seropositivity and thyroid dysfunction. METHODS: We performed an age- and gender-matched case-control study of 176 patients suffering from hypothyroidism (n = 161) or hyperthyroidism (n = 15) and 528 control subjects without these diseases in a public hospital in Durango City, Mexico. Anti-Toxoplasma IgG antibodies were determined in sera from cases and controls using a commercially available enzyme-linked immunoassay. RESULTS: Anti-T. gondii IgG antibodies were found in 11 (6.3%) of 176 patients suffering from thyroid dysfunction and in 48 (9.1%) of 528 control subjects (OR = 0.66; 95% CI: 0.33-1.31; P = 0.23). Stratification by two groups of age (50 years and younger, and 51 year and older) showed that the youngest group of patients with thyroid dysfunction had a significantly lower seroprevalence of T. gondii infection than its age- and gender-matched control group (1/83: 1.2% vs 23/257: 8.6%; OR = 0.12; 95% CI: 0.01-0.93; P = 0.01). This stratification also showed that the youngest group of patients with hypothyroidism had a significantly lower seroprevalence of T. gondii infection than its age- and gender matched control group (0/75: 0% vs 21/233: 9.0%; P = 0.003). CONCLUSIONS: Our results suggest that thyroid dysfunction is not associated with seropositivity to T. gondii in general; however, in young (50 years or less) patients, a negative association between infection and thyroid dysfunction and hypothyroidism was found. Further research to confirm this negative association is needed.


Assuntos
Hipotireoidismo/diagnóstico , Toxoplasmose/diagnóstico , Adulto , Anticorpos Antiprotozoários/sangue , Estudos de Casos e Controles , Ensaio de Imunoadsorção Enzimática , Feminino , Hospitais Públicos , Humanos , Hipotireoidismo/complicações , Hipotireoidismo/epidemiologia , Imunoglobulina G/sangue , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Estudos Soroepidemiológicos , Toxoplasma/imunologia , Toxoplasma/isolamento & purificação , Toxoplasmose/complicações , Toxoplasmose/epidemiologia
6.
Infect Immun ; 87(8)2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31182619

RESUMO

Toxoplasma gondii, a common neurotropic parasite, is increasingly being linked to neuropsychiatric disorders, including schizophrenia, Alzheimer's disease, and Parkinson's disease. However, the pathogenic mechanisms underlying these associations are not clear. Toxoplasma can reside in the brain for extensive periods in the form of tissue cysts, and this process requires a continuous immune response to prevent the parasite's reactivation. Because neuroinflammation may promote the onset and progression of neurodegenerative diseases, we investigated neurodegeneration-associated pathological changes in a mouse model of chronic Toxoplasma infection. Under conditions of high-grade chronic infection, we documented the presence of neurodegeneration in specific regions of the prefrontal cortex, namely, the anterior cingulate cortex (ACC) and somatomotor cortex (SC). Neurodegeneration occurred in both glutamatergic and GABAergic neurons. Neurons that showed signs of degeneration expressed high levels of CX3CL1, were marked by profoundly upregulated complement proteins (e.g., C1q and C3), and were surrounded by activated microglia. Our findings suggest that chronic Toxoplasma infection leads to cortical neurodegeneration and results in CX3CL1, complement, and microglial interactions, which are known to mediate the phagocytic clearance of degenerating neurons. Our study provides a mechanistic explanation for the link between Toxoplasma infection and psychiatric disorders.


Assuntos
Encéfalo/parasitologia , Ativação do Complemento/fisiologia , Microglia/fisiologia , Doenças Neurodegenerativas/etiologia , Toxoplasmose/complicações , Animais , Quimiocina CX3CL1/fisiologia , Doença Crônica , Modelos Animais de Doenças , Feminino , Camundongos , Ácido gama-Aminobutírico/fisiologia
7.
Acta Trop ; 196: 165-171, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31102579

RESUMO

In the past decade, Toxoplasma gondii infection has been recognized as a potential risk for many psychiatric and neurological disorders. We performed this systematic review and meta-analysis to evaluate the association between Toxoplasma infection and Parkinson's and Alzheimer's diseases. PubMed, Web of science, Scopus and Embase databases were searched up to September 30, 2018 for studies that reported risk of Parkinson's and Alzheimer's diseases associated with Toxoplasma infection. We used a random effects meta-analysis model to generate the pooled odds ratio (OR) with 95% confidence intervals (CIs). Eleven studies, including seven studies for Parkinson's disease (428 patients and 540 controls) and four studies for Alzheimer's disease (301 patients and 313 controls), were included in the meta-analysis. We found that there was no statistically significant association between Toxoplasma infection, as determined by IgG serology, IgM serology, and PCR with increased risk of Parkinson's disease (OR, 1.14; 95% CI, 0.78-1.68), (OR, 1.61; 95% CI, 0.33-7.76) and (OR, 1.87; 95% CI, 0.43-8.05), respectively. The OR for association of Toxoplasma infection, based on IgG serology with Alzheimer's patients, compared to control group, was (OR, 1.38; 95% CI, 0.99-1.92), demonstrating a marginally significant association between Toxoplasma infection and Alzheimer's disease. Our findings do not support a general hypotheses regarding an associative relationship between Toxoplasma infection and Parkinson's disease, but do support a marginally significant association between Toxoplasma infection and Alzheimer's disease; this association should be investigated further through longitudinal and experimental studies.


Assuntos
Doença de Alzheimer/etiologia , Anticorpos Antiprotozoários/sangue , Doença de Parkinson/etiologia , Toxoplasmose/complicações , Humanos , Fatores de Risco
9.
PLoS Negl Trop Dis ; 13(4): e0007306, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30969961

RESUMO

BACKGROUND: A few studies investigated the relationship between toxoplasmosis and mental disorders, such as obsessive compulsive disorder (OCD). However, the specific nature of the association between Toxoplasma gondii (T. gondii) infection and OCD is not yet clear. The aim of this study was to collect information on the relationship between OCD and toxoplasmosis and assess whether patients with toxoplasmosis are prone to OCD. METHODS: For the purpose of this study, 6 major electronic databases and the Internet search engine Google Scholar were searched for the published articles up to July 30th, 2018 with no restriction of language. The inverse variance method and the random effect model were used to combine the data. The values of odds ratio (OR) were estimated at 95% confidence interval (CI). RESULTS: A total of 9 case-control and 3 cross-sectional studies were included in our systematic review. However, 11 of these 12 articles were entered into the meta-analysis containing 9873 participants, out of whom 389 were with OCD (25.96% positive for toxoplasmosis) and 9484 were without OCD (17.12% positive for toxoplasmosis). The estimation of the random effect model indicated a significant common OR of 1.96 [95% CI: 1.32-2.90]. CONCLUSION: This systematic review and meta-analysis revealed that toxoplasmosis could be as an associated factor for OCD (OR = 1.96). However, further prospective investigations are highly recommended to illuminate the underlying pathophysiological mechanisms of T. gondii infection in OCD and to better investigate the relationship between OCD and T. gondii infection.


Assuntos
Suscetibilidade a Doenças , Transtorno Obsessivo-Compulsivo/etiologia , Toxoplasmose/complicações , Anticorpos Antiprotozoários/sangue , Estudos de Casos e Controles , Estudos Transversais , Humanos , Transtorno Obsessivo-Compulsivo/parasitologia , Razão de Chances , Toxoplasma/imunologia
10.
Biomed Res Int ; 2019: 8186017, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30931331

RESUMO

Background: Several studies have investigated the association between Toxoplasma gondii (T. gondii) infection and risk of Parkinson's disease (PD) with inconsistent results. Clarifying this relation might be useful for better understanding of the risk factors and the relevant mechanisms of PD, thus a meta-analysis was conducted to explore whether exposure to T. gondii is associated with an increased risk of PD. Methods: We conducted this meta-analysis according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. A rigorous literature selection was performed by using the databases of PubMed, Embase, Web of Science, Cochrane Library, and ScienceDirect. Odds ratio (OR) and corresponding 95% confidential interval (CI) were pooled by using fixed-effects models. Sensitivity analysis, publication bias test, and methodological quality assessment of studies were also performed. Results: Seven studies involving 1086 subjects were included in this meta-analysis. Pooled data by using fixed-effects models suggested both latent infection (OR, 1.17; 95% CI, 0.86 to 1.58; P=0.314) and acute infection (OR, 1.13; 95% CI, 0.30 to 4.35; P=0.855) were not associated with PD risk. Stable and robust estimates were confirmed by sensitivity analysis. No publication bias was found by visual inspection of the funnel plot, Begg's, and Egger's test. Conclusions: This meta-analysis does not support any possible association between T. gondii infection and risk of PD. Researches are still warranted to further explore the underlying mechanisms of T. gondii in the pathogenesis of PD and their causal relationship.


Assuntos
Doença de Parkinson/epidemiologia , Toxoplasmose/epidemiologia , Humanos , Doença de Parkinson/complicações , Doença de Parkinson/microbiologia , Doença de Parkinson/patologia , Fatores de Risco , Toxoplasma/patogenicidade , Toxoplasmose/complicações , Toxoplasmose/microbiologia , Toxoplasmose/patologia
11.
Acta Trop ; 193: 211-216, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30871988

RESUMO

Parasitic diseases are among the major health problems of various societies, especially people in developing countries, causing high economic and mortality burdens. Many researchers have reported that awareness and knowledge of a disease are effective in preventing and controlling the disease. The aim of the present study was to assess the knowledge and attitudes on parasitic diseases of the population in Ahvaz County, southwestern Iran. This community-based cross-sectional study was carried out in 2017 on 3500 subjects above 15 years old of Ahvaz County. First, participants were provided the necessary information regarding the study, and then data were collected using a constructed questionnaire. Of the 3500 participants, 1732 (49.5%) were female and 1768 (50.5%) were male, 348 (9.9%) said that they had been infected with a parasitic disease at least once in their lifetime. Almost half of the participants (42.8%) knew malaria was a parasitic disease and 50.2%, 48.9%, and 41.5% were aware of the possible transmission of parasitic diseases through uncooked meat, dirty hands, and close contact with animals. Regarding clinical symptoms, 73.2% and 32.6% of the respondents knew diarrhea and abdominal pain respectively were clinical symptoms of intestinal parasitic diseases. However, only 31.3% were aware of the close contact with cats and the risk of human toxoplasmosis and possible abortion in pregnant women. The findings reveal that the attitude of most participants toward treatment was positive (93.9%), but only 47.5% of believed that disinfecting fruits and vegetables could prevent intestinal parasitic infections.


Assuntos
Países em Desenvolvimento , Conhecimentos, Atitudes e Prática em Saúde , Doenças Parasitárias/complicações , Doenças Parasitárias/transmissão , Dor Abdominal/parasitologia , Aborto Espontâneo/parasitologia , Adolescente , Adulto , Idoso , Animais , Gatos , Estudos Transversais , Diarreia/parasitologia , Feminino , Humanos , Enteropatias Parasitárias/complicações , Enteropatias Parasitárias/prevenção & controle , Enteropatias Parasitárias/transmissão , Irã (Geográfico) , Malária/parasitologia , Masculino , Pessoa de Meia-Idade , Doenças Parasitárias/tratamento farmacológico , Doenças Parasitárias/prevenção & controle , Gravidez , Inquéritos e Questionários , Toxoplasmose/complicações , Toxoplasmose/transmissão , Adulto Jovem
12.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 30(6): 682-684, 2019 Feb 19.
Artigo em Chinês | MEDLINE | ID: mdl-30891985

RESUMO

OBJECTIVE: To investigate the seroprevalence of Toxoplasma gondii infection in patients with gynecological malignant tumors, so as to provide the evidence for the control of T. gondii infections in the patients with gynecological malignant tumors. METHODS: A total of 327 patients with gynecological malignant tumors were recruited, and 200 women with normal healthy examinations were served as controls. The serum anti-T. gondii IgG and IgM antibodies were detected by using ELISA, and the seroprevalence was compared between the patients and controls, and among various types of cancers. RESULTS: The overall seroprevalence of T. gondii was 26.91% (88/327) in the patients with gynecological malignant tumors, which was significantly higher than that (5.00%) in the healthy volunteers (χ2 = 39.36, P<0.01) . The prevalence of anti-T. gondii IgG antibody in the cancer patients was significantly higher than that in the controls (26.30% vs. 5.00%; χ2 = 37.79, P<0.01), while the prevalence of anti-T. gondii IgM antibody in the cancer patients was not significantly different from that in the controls (0.92% vs. 0; corrected χ2 = 0.58, P > 0.01) . The positive rates of anti-T. gondii IgG antibody were 27.68%, 25.47% and 25.69% in ovarian cancer, cervical cancer, and breast cancer patients respectively, which were all significantly higher than those in the controls (χ2 = 32.35, 27.32 and 28.00; all P values<0.01); however, there was no significant difference among the various types of cancer patients in the positive rates of anti-T. gondii IgG antibody (χ2 = 0.17, P > 0.05) . In addition, the positive rates of anti-T. gondii IgM antibody were 1.79%, 0 and 0.92% in ovarian cancer, cervical cancer and breast cancer patients respectively, which was not significantly different from those in the controls (all P values > 0.05). CONCLUSIONS: The seroprevalence of T. gondii infection is high in the patients with gynecological malignant tumors. It is suggested that the prevention and control of T. gondii infection should be strengthened in these patients.


Assuntos
Neoplasias dos Genitais Femininos , Toxoplasma , Toxoplasmose , Anticorpos Antiprotozoários/sangue , Feminino , Neoplasias dos Genitais Femininos/complicações , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Estudos Soroepidemiológicos , Inquéritos e Questionários , Toxoplasmose/sangue , Toxoplasmose/complicações
13.
J Craniofac Surg ; 30(4): e353-e355, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30865129

RESUMO

Submental mass secondary to toxoplasmosis is a rare condition and physicians rarely consider its diagnosis. The presented case reports a 43-year-old woman referred with a constant, painless, edema located in the submental area for 2 weeks. Diagnosis of toxoplasmosis was established by the positive serological finding after an accurate medical history of the infectious disease specialist (the patient had eaten raw meat). The patient underwent antimicrobial therapy with resolution of lymphadenopathy. According to review of literature, the article aims to remind clinicians, maxillofacial surgeons, and otolaryngologists that a neck mass can be related to the infectious cause with involving of lymph node like as toxoplasmosis. Therefore, a serological diagnosis should be considered before moving to needle biopsy or excisional biopsy of neck mass.


Assuntos
Linfadenopatia , Toxoplasmose , Adulto , Edema/etiologia , Edema/fisiopatologia , Feminino , Humanos , Linfadenopatia/diagnóstico , Linfadenopatia/etiologia , Pescoço/fisiopatologia , Toxoplasmose/complicações , Toxoplasmose/diagnóstico
14.
BMC Infect Dis ; 19(1): 91, 2019 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-30683065

RESUMO

BACKGROUND: Making a definite diagnosis of infectious uveitis is a challenging task because many other infectious, and non-infectious uveitis, may have similar non-specific symptoms and overlapping clinical appearances. Co-infections in immunocompetent patients are not frequently proved with traditional serologic-diagnostic tools. METHODS: Descriptive transversal study, in a Uveitis Service of an Ophthalmology Reference Center, in Bogotá, Colombia, from July 2014 to February 2016. Aqueous humor (AH) and/or vitreous fluid, blood and serum samples were collected from consecutive patients suspected of having infectious uveitis. The diagnosis of ocular toxoplasmosis (OT) was confirmed by the Goldmann-Witmer coefficient (GWC) and by polymerase chain reaction (PCR). Differential diagnosis by PCR in AH was done for viral origin such as Cytomegalovirus (CMV), Herpes simplex virus type 1 (HSV1), Herpes simplex virus type 2 (HSV2), Varicella zoster virus (VZV), Epstein-Barr virus (EBV) and Mycobacterium tuberculosis. RESULTS: In 66 Colombian patients with uveitis of presumed infectious origin: 22 (33.3%) were confirmed as OT, 16 (24.2%) as undetermined OT, five (7.5%) as co-infections and 23 (34.8%) as other uveitis. Toxoplasma coinfection with M. tuberculosis was identified in one case by PCR and in four cases with HSV by GWC. The initial clinical diagnosis changed, after laboratory examination, in 21 cases (31.8%). CONCLUSIONS: Clinical diagnosis can be changed by laboratory examination in a significant proportion of cases of uveitis. Diagnosis of OT should combine the use of PCR and GWC to reach the maximum of confirmation of cases. The use of multiple laboratory methods is necessary to identify co-infections and viral infections that can mimic OT in immunocompetent patients.


Assuntos
Coinfecção/diagnóstico , Infecções Oculares Parasitárias/diagnóstico , Infecções Oculares Virais/diagnóstico , Infecções por Herpesviridae/diagnóstico , Imunocompetência , Toxoplasmose/diagnóstico , Adolescente , Adulto , Idoso , Coinfecção/epidemiologia , Coinfecção/imunologia , Colômbia/epidemiologia , Citomegalovirus/genética , DNA Viral/análise , Diagnóstico Diferencial , Infecções Oculares Parasitárias/complicações , Infecções Oculares Virais/complicações , Infecções Oculares Virais/imunologia , Infecções Oculares Virais/virologia , Feminino , Infecções por Herpesviridae/complicações , Infecções por Herpesviridae/imunologia , Infecções por Herpesviridae/virologia , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Toxoplasmose/complicações , Toxoplasmose/imunologia , Adulto Jovem
15.
Eur J Clin Microbiol Infect Dis ; 38(2): 305-317, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30470966

RESUMO

Toxoplasmosis was linked to impairment in brain function, encompassing a wide range of behavioral and neuropsychiatric changes. Currently, the precise localization of Toxoplasma gondii in the human brain is limited and the parasite DNA was not found in population-based screening of autopsy cases. The aim of proposed study was to identify the presence of parasite DNA within the brain and its association with risky behavior and alcohol consumption in postmortem examination. Preliminarily, 102 cases with certain circumstances of death at time of forensic autopsy was included. Due to high risk of bias, the females were excluded from the analysis and final study group consists 97 cases divided into three groups: risky behavior, inconclusively risky behavior, and control group. The obtained tissue samples for Nested PCR covered four regions of the brain: symmetric left/right and anterior/posterior horns of lateral ventricles comprising lining ependyma and hippocampus. The second type of material comprised blood evaluated for antibodies prevalence using ELISA and alcohol concentration using HS-GC-FID. Analysis demonstrated 16.5% prevalence concerning the parasite DNA presence in examined brain tissue samples without specific distribution and association with age at death or days after death until an autopsy was performed. Results have shown correlation between occurrence of risky behavior leading to death and higher proportions of positive parasite DNA presence within the brain. Correlation was not observed between parasite DNA presence and excessive alcohol consumption. Conducted screening demonstrated correlation between parasite DNA presence in the brain with risky behavior and provided new information on possible effects of latent toxoplasmosis.


Assuntos
Encéfalo/parasitologia , Comportamento Perigoso , Toxoplasmose/complicações , Toxoplasmose/diagnóstico , Anticorpos Antiprotozoários/sangue , Autopsia , Concentração Alcoólica no Sangue , Encéfalo/patologia , DNA de Protozoário/genética , Ensaio de Imunoadsorção Enzimática , Humanos , Masculino , Polônia , Reação em Cadeia da Polimerase , Prevalência , Toxoplasma/genética
16.
Behav Brain Res ; 359: 737-748, 2019 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-30253194

RESUMO

Toxoplasma gondii is a protozoan parasite that can cause a latent infection in the central nervous system, leading to neurobehavioral abnormalities in the host. However, the mechanism underlying these changes remains relatively unexplored. In this study, we detected behavioral changes, pathological injury, secretion of neurotransmitters and related signal pathway in mice infected by T. gondii using behavioral test, histopathology, immunofluorescence staining, western blotting, HPLC and real time PCR. Mice showed neurobehavioral disturbances two months after infection with T. gondii. Histopathology revealed the activation of astrocytes and microglia, apoptosis of neurons and decreases in synapses in the brain of infected mice. Excessive secretion of cytokines and chemokines was detected in the brains of mice infected by T. gondii compared to uninfected mice. Furthermore, T. gondii infection led to abnormalities in neurotransmitters and the activation of NF-κB and dopamine (DA) signaling pathways in the infected mice. In conclusion, excessive activation of the inflammation in the brain could induce neuronal apoptosis in mice chronically infected with T. gondii. Dysregulation of the dopaminergic neurotransmitter could provide an explanation of neurobehavioral disorders in infected hosts.


Assuntos
Inflamação/etiologia , Inflamação/metabolismo , Transtornos Mentais/etiologia , Transtornos do Humor/etiologia , Neurotransmissores/metabolismo , Toxoplasmose/complicações , Animais , Proteínas de Ligação ao Cálcio/metabolismo , Doença Crônica , Modelos Animais de Doenças , Comportamento Exploratório/fisiologia , Feminino , Regulação da Expressão Gênica , Proteína Glial Fibrilar Ácida/metabolismo , Elevação dos Membros Posteriores/fisiologia , Marcação In Situ das Extremidades Cortadas , Inflamação/parasitologia , /parasitologia , Aprendizagem em Labirinto/fisiologia , Transtornos Mentais/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Proteínas dos Microfilamentos/metabolismo , Transtornos do Humor/parasitologia , Fosfopiruvato Hidratase/metabolismo , Sinaptotagmina I/metabolismo
17.
Int J Infect Dis ; 79: 131-133, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30529368

RESUMO

Myopericarditis is a rare but well-documented clinical presentation of primary Toxoplasma gondii infection in immunocompetent patients. Here, early detection of Toxoplasma DNA in the peripheral blood by PCR allowed the diagnosis of acute toxoplasmosis while serological tests were negative. Additional serological evaluations 2 weeks later confirmed the diagnosis and showed that cardiac manifestations occurred before seroconversion. This highlights the importance of a second serological control in the case of a suspected active infection. Overall, we show here that PCR testing for Toxoplasma is a sensitive and straightforward alternative to serological examinations.


Assuntos
DNA de Protozoário/isolamento & purificação , Miocardite/etiologia , Toxoplasmose/diagnóstico , Anticorpos Antiprotozoários/sangue , Diagnóstico Precoce , Humanos , Masculino , Miocardite/diagnóstico , Patologia Molecular , Reação em Cadeia da Polimerase , Sensibilidade e Especificidade , Soroconversão , Testes Sorológicos , Toxoplasma/isolamento & purificação , Toxoplasmose/sangue , Toxoplasmose/complicações , Adulto Jovem
18.
Glia ; 67(5): 844-856, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30588668

RESUMO

Alzheimer's disease (AD) is the leading cause of age-related neurodegeneration and is characterized neuropathologically by the accumulation of insoluble beta-amyloid (Aß) peptides. In AD brains, plaque-associated myeloid (PAM) cells cluster around Aß plaques but fail to effectively clear Aß by phagocytosis. PAM cells were originally thought to be brain-resident microglia. However, several studies have also suggested that Aß-induced inflammation causes peripheral monocytes to enter the otherwise immune-privileged brain. The relationship between AD progression and inflammation in the brain remains ambiguous because microglia and monocyte-derived macrophages are extremely difficult to distinguish from one another in an inflamed brain. Whether PAM cells are microglia, peripheral macrophages, or a mixture of both remains unclear. CD11a is a component of the ß2 integrin LFA1. We have determined that CD11a is highly expressed on peripheral immune cells, including macrophages, but is not expressed by mouse microglia. These expression patterns remain consistent in LPS-treated inflamed mice, as well as in two mouse models of AD. Thus, CD11a can be used as a marker to distinguish murine microglia from infiltrating peripheral immune cells. Using CD11a, we show that PAM cells in AD transgenic brains are comprised entirely of microglia. We also demonstrate a novel fluorescence-assisted quantification technique (FAQT), which reveals a significant increase in T lymphocytes, especially in the brains of female AD mice. Our findings support the notion that microglia are the lead myeloid players in AD and that rejuvenating their phagocytic potential may be an important therapeutic strategy.


Assuntos
Doença de Alzheimer/patologia , Antígeno CD11a/metabolismo , Microglia/metabolismo , Microglia/patologia , Células Mieloides/metabolismo , Algoritmos , Doença de Alzheimer/genética , Doença de Alzheimer/cirurgia , Animais , Animais Recém-Nascidos , Transplante de Medula Óssea , Encéfalo/metabolismo , Encéfalo/patologia , Receptor 1 de Quimiocina CX3C/genética , Receptor 1 de Quimiocina CX3C/metabolismo , Células Cultivadas , Regulação da Expressão Gênica no Desenvolvimento/genética , Humanos , Inflamação/etiologia , Lipopolissacarídeos/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Microglia/efeitos dos fármacos , Células Mieloides/efeitos dos fármacos , Oxirredutases/genética , Oxirredutases/metabolismo , Receptores CCR2/genética , Receptores CCR2/metabolismo , Toxoplasmose/complicações
19.
BMC Infect Dis ; 18(1): 629, 2018 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-30522446

RESUMO

BACKGROUND: Previous studies have suggested that Toxoplasma gondii (T. gondii) infection might be associated with fatty liver disease. However, the relationship between non-alcoholic fatty liver disease (NAFLD) and T. gondii infection has not been investigated in a large population. We aimed to study the relationship between those two diseases using a population-based dataset from the United States. METHODS: The data were collected from the third National Health and Nutrition Examination Survey (NHANES III) between 1988 and 1994. Statistical analysis was applied to compare the prevalence of NAFLD in anti-T. gondii antibody-positive participants with antibody-negative ones. RESULTS: A total of 9465 persons with a mean age of 44.33 ± 16.21 years, 46.9% of which were males, were included in the final analysis. Their mean BMI was 27.60 ± 5.96 kg/m2. A total of 2520 participants (26.62%) were positive for the T. gondii antibody. There was an increasing trend of seroprevalence of T. gondii with age (P for trend < 0.001). The incidence of NAFLD in the seropositive group was higher than that in the seronegative group (27.10% vs 23.40%, p < 0.001). In addition to this, metabolic biomarkers, including serum lipid, fasting blood-glucose, and uric acid were also significantly higher in the seropositive group. However, multivariate analysis revealed that T. gondii infection was not an independent risk factor for NAFLD. Age was independently correlated with both the prevalence of T. gondii and NAFLD. CONCLUSIONS: Patients with T. gondii infection may have a higher prevalence of NAFLD. Age may have an effect on the increase of NAFLD in the T. gondii seropositive population.


Assuntos
Hepatopatia Gordurosa não Alcoólica , Toxoplasmose , Adulto , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/complicações , Hepatopatia Gordurosa não Alcoólica/epidemiologia , Toxoplasmose/complicações , Toxoplasmose/epidemiologia , Estados Unidos/epidemiologia
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