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1.
Chemosphere ; 250: 126293, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32234621

RESUMO

Pesticides introduced inadvertently or deliberately into environment by global agricultural practices have caused growing public concern, therefore the search of approaches for elimination of such xenobiotics should be motivated. The degradation of hydrophobic pesticides including chlorpyrifos, dicofol and cypermethrin were assayed with the white-rot fungus Trametes versicolor. Experiments were set at realistic concentration as 5 µg L-1, and both culture medium and biologic matrix were analyzed for pollutants residues. Results showed that the first step was due to a fast adsorption, which also played an important role, accounting for more than 90% removal in average. Then mass balances proposal evidenced the biodegradation of the adsorbed pollutants, demonstrating efficient depletion as 94.7%, 87.9% and 93.1%, respectively. Additionally, the related degradation metabolites were identified using ultra performance liquid chromatography coupled to high resolution mass spectrometry. Two compounds, namely O,O-diethyl thiophosphate and diethyl phosphate were detected as transformation products of chlorpyrifos, whereas dicofol was degraded into benzaldehyde that is first time to be reported. It also confirms the degradation capability of T. versicolor. Our results suggest that T. versicolor is a potential microorganism for bioremediation of hydrophobic pesticide contaminated environments.


Assuntos
Biodegradação Ambiental , Praguicidas/metabolismo , Trametes/metabolismo , Adsorção , Agaricales/metabolismo , Agricultura , Clorpirifos/metabolismo , Espectrometria de Massas
2.
Prep Biochem Biotechnol ; 50(1): 10-17, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31430215

RESUMO

Due to wide application of laccase, many researchers have shown great interest in over production of white-rot fungi laccase by co-culture. In this study, a white-rot fungus Trametes hirsuta SSM-3, and a yeast Sporidiobolus pararoseus SSM-8 were isolated and identified from Mulberry fruit. The capacity of S. pararoseus to enhance laccase production was remarkable in T. hirsuta, yielding 31777 ± 742 U/L, about 9.9 times higher than the result from the monoculture. The stimulatory factor in the S. pararoseus cells might be temperature-sensitive. The laccase production was enhanced by oil-extract of S. pararoseus and ß-carotene induction. The amylase activity was decreased rapidly when strain S. pararoseus SSM-8 was inoculated. The glucose deprivation was occurred both in the mono-culture and co-culture process, and S. pararoseus propagated slowly in co-culture all the time. Native-PAGE revealed an increase of laccase-1(lac-1) level and a laccase-3 (lac-3) in the co-culture. Therefore, it was concluded that competition for resources between the co-cultured microbes leaded to amylase decreasing and the enhanced production of laccase. This conclusion was helpful for the development of laccase fermentation industry because it provided an effective, simple and economic method to improve the yield of laccase.


Assuntos
Proteínas Fúngicas/metabolismo , Lacase/metabolismo , Trametes/metabolismo , Leveduras/metabolismo , Técnicas de Cocultura/métodos , Fermentação , Microbiologia Industrial/métodos , Morus/microbiologia , beta Caroteno/metabolismo
3.
Chemosphere ; 238: 124572, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31422312

RESUMO

The wastewaters from distilleries of winemaking by-products, a scarcely studied type of vinasse, were treated by white-rot fungal strains from species Irpex lacteus, Ganoderma resinaceum, Trametes versicolor, Phlebia rufa and Bjerkandera adusta. The main objectives of this study were to evaluate fungal performance during vinasse biodegradation, their enzyme patterns and ecotoxicity evolution throughout treatment. Despite all strains were able to promote strong (>80%) dephenolization and reduction of total organic carbon (TOC), P. rufa was less affected by vinasse toxicity and exhibit better decolorization. In batch cultures at 28 °C and pH 4.0, the first phase of P. rufa biodegradation kinetics was characterized by strong metabolic activity with simultaneous depletion of TOC, phenolics and sugars. The main events of second phase are the increase of peroxidases production after the peak of laccase activity, and strong color removal. At the end of treatment, it was observed highly significant (p < 0.001) abatement of pollution parameters (83-100% removal). Since water reclamation and reuse for e.g. crop irrigation is a priority issue, vinasse ecotoxicity was assessed with bioindicators representing three different phylogenetic and trophic levels: a marine bacterium (Aliivibrio fischeri), a freshwater microcrustacean (Daphnia magna) and a dicotyledonous macrophyte (Lepidium sativum). It was observed significant (p < 0.05) reduction of initial vinasse toxicity, as evaluated by these bioindicators, deserving special mention an almost complete phytotoxicity elimination.


Assuntos
Aliivibrio fischeri/crescimento & desenvolvimento , Coriolaceae/metabolismo , Daphnia/crescimento & desenvolvimento , Lepidium sativum/crescimento & desenvolvimento , Polyporales/metabolismo , Trametes/metabolismo , Águas Residuárias/química , Águas Residuárias/toxicidade , Aliivibrio fischeri/metabolismo , Animais , Biodegradação Ambiental , Daphnia/metabolismo , Destilação , Biomarcadores Ambientais/efeitos dos fármacos , Lacase/metabolismo , Lepidium sativum/metabolismo , Peroxidases/metabolismo , Fenóis/metabolismo , Filogenia
4.
J Biotechnol ; 307: 55-62, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31545972

RESUMO

Carboxylic acid reductases (CARs) are attracting burgeoning attention as biocatalysts for organic synthesis of aldehydes and their follow-up products from economic carboxylic acid precursors. The CAR enzyme class as a whole, however, is still poorly understood. To date, relatively few CAR sequences have been reported, especially from fungal sources. Here, we sought to increase the diversity of the CAR enzyme class. Six new CAR sequences from the white-rot fungus Pycnoporus cinnabarinus were identified from genome-wide mining. Genome and gene clustering analysis suggests that these PcCAR enzymes play different natural roles in Basidiomycete systems, compared to their type II Ascomycete counterparts. The cDNA sequences of all six Pccar genes were deduced and analysis of their corresponding amino acid sequence showed that they encode for proteins of similar properties that possess a conserved modular functional tri-domain arrangement. Phylogenetic analyses showed that all PcCAR enzymes cluster together with the other type IV CARs. One candidate, PcCAR4, was cloned and over-expressed recombinantly in Escherichia coli. Subsequent biotransformation-based screening with a panel of structurally-diverse carboxylic acid substrates suggest that PcCAR4 possessed a more pronounced substrate specificity compared to previously reported CARs, preferring to reduce sterically-rigid carboxylic acids such as benzoic acid. These findings thus present a new functionally-distinct member of the CAR enzyme class.


Assuntos
Oxirredutases/metabolismo , Pycnoporus/enzimologia , Aldeídos/metabolismo , Ácidos Carboxílicos/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas Fúngicas/classificação , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Oxirredutases/classificação , Oxirredutases/genética , Filogenia , Pycnoporus/genética , Especificidade por Substrato , Trametes/metabolismo
5.
Int J Med Mushrooms ; 21(8): 735-753, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31679282

RESUMO

Basidiomycetes of various species and their wide range of pharmaceuticaly interesting products in the past decades represents one of the most attractive groups of natural products in Asia and North America. Production of mushroom fruit bodies using farming technology is hardly covering the market. Development of comprehensive submerged technologies in stirred tank and air lift bioreactors are the most promising technologies for fast and large-amount cultivation of medicinal mushroom biomass and its pharmaceutically active products. Research in physiology, basic and applied studies in mushroom metabolism, process engineering aspects, and clinical studies in the past two decades represent a large cotribution to the development of this potential, which initiates the development of new drugs and some very attractive over-the-counter human and veterinary remedies. The current article is an overview of the most relevant engineering achievements in submerged cultivation of some medicinal mushrooms-Grifola frondosa, Trametes versicolor, Hericium erinaceus, and Cordyceps militaris-and some other species biomass production in bioreactors.


Assuntos
Basidiomycota/crescimento & desenvolvimento , Produtos Biológicos/metabolismo , Reatores Biológicos , Agaricales , Agricultura , Ásia , Basidiomycota/metabolismo , Biomassa , Cordyceps/crescimento & desenvolvimento , Cordyceps/metabolismo , Engenharia , Grifola/crescimento & desenvolvimento , Grifola/metabolismo , Trametes/crescimento & desenvolvimento , Trametes/metabolismo
6.
Int J Mol Sci ; 20(17)2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31454912

RESUMO

Fusarium langsethiae is amongst the most recently discovered pathogens of small grains cereals. F. langsethiae is the main producer, in Europe, of T2 and HT-toxins in small grain cereals, albeit often asymptomatic; this makes its control challenging. The European Union (EU) is pushing hard on the use of biocontrol agents to minimize the use of fungicides and pesticides, which are detrimental to the environment and responsible for serious pollution of the soil and superficial water. In line with EU directives (e.g., 128/2009), here we report the use of protein fractions, purified from the culture filtrate of the basidiomycete Trametes versicolor, for controlling F. langsethiae. T. versicolor, a so-called medicinal mushroom which is applied as a co-adjuvant in oncology and other pathologies as a producer of biological response modifiers. In this study, the exo-proteome of T. versicolor proved highly efficient in inhibiting the growth of F. langsethiae and the biosynthesis of the T2 toxin. Results are promising for its future use as a sustainable product to control F. langsethiae infection in cereals under field conditions.


Assuntos
Agaricales/metabolismo , Antibiose , Grão Comestível/microbiologia , Fusarium/fisiologia , Proteoma , Trametes/metabolismo , Bioensaio , Micotoxinas/biossíntese
7.
Sci Total Environ ; 672: 732-742, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30974363

RESUMO

As a result of anthropization and industrialization, northern remote communities face issues of soil contamination by mixtures of organic and inorganic contaminants. Soil bioremediation in cold environments is particularly challenging because of slower degradation rates, slower production of biomass for phytoextraction of trace elements (TEs), and remoteness, which can complicate logistics and inflate costs. This study evaluated a decontamination approach integrating indigenous willows, fungi and compost in a northern community. The site was a waste oil pit and its soil was initially contaminated with petroleum hydrocarbons (PHC) exceeding 200 g kg-1 and TEs including As, Cd, Co, Cr, Cu, Pb and Zn. In under five years, 65 and 75% of PHC (C6-C50 and >C50) were degraded, compared to 27 and 13% for the untreated control soil. We found contrasting TE translocation patterns to the aboveground biomass for the willow species used (Salix planifolia and Salix alaxensis), as well as distinctive rooting strategies. Hazard quotients were calculated to assess the risk plant material could pose to local wildlife. The highest TE concentration measured was Zn in S. planifolia, which exceeded Canadian soil guidelines. Results indicate toxicity risks to animals linked to TEs in Salix spp. leaves is generally unlikely. The fungus Trametes versicolor inoculated into the soil did not fruit, however fruiting bodies of Psathyrella sp. were observed consistently (four out of five years). Biological tests indicated that in five growing seasons soil toxicity significantly decreased compared to the untreated soil used as control. This was demonstrated by vegetation cover (137 vs 11% cover), toxicity assays on earthworms (Eisenia andrei) (0 vs 33% mortality) and barley seed germination (Hordeum vulgare) (86 vs 62% germination). The proposed decontamination approach, without the use of synthetic fertilizers, is promising for the PHC remediation of mixed-contaminants on cold climate sites.


Assuntos
Biodegradação Ambiental , Compostagem , Poluição por Petróleo/análise , Petróleo/metabolismo , Poluentes do Solo/metabolismo , Animais , Canadá , Temperatura Baixa , Hidrocarbonetos/metabolismo , Oligoquetos/metabolismo , Salix , Solo , Trametes/metabolismo
8.
Chemosphere ; 224: 743-750, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30851526

RESUMO

As a typical class of environmental endocrine disruptors, bisphenol A poses a potential threat to the sustainable survival and reproduction of living beings and human beings. In this study, the interaction between Trametes versicolor laccase and bisphenol A (BPA) was studied by molecular docking simulation, and the catalytic degradation of BPA was verified by experiments. The conditions for the laccase production of T. versicolor were optimized by orthogonal design, and the degradation of BPA was studied using its crude enzyme solution. The optimum degradation conditions were obtained by response surface methodology (RSM). Ultimately, the transformation products after 3 and 6 h of reaction were detected by gas chromatography-mass spectrometry. Docking results demonstrated that the reaction between laccase and BPA was spontaneous, and the degradation rate in 24 h reached 88.76%. RSM results showed that the highest BPA degradation rate of 97.68% was reached after 1 h reaction at 44.6 °C, 5 mg/L initial BPA concentration, and pH 5.20. The intermediate products of BPA catalyzed by laccase included ethylbenzene, p-xylene, and cyclohexanone 1-methyl-4-isopropenyl-2-cyclohexenol. This finding reveals that BPA degradation by the crude laccase from T. versicolor starts from the C atoms between two benzene rings that connect BPA. Compared with expensive pure enzyme, the crude laccase solution prepared by T. versicolor showed greater efficiency in BPA degradation. This work provides theoretical references and experimental methods for the biological processing of harmful pollutants.


Assuntos
Compostos Benzidrílicos/metabolismo , Biodegradação Ambiental , Disruptores Endócrinos/metabolismo , Lacase/metabolismo , Fenóis/metabolismo , Trametes/metabolismo , Agaricales/metabolismo , Derivados de Benzeno/análise , Catálise , Poluentes Ambientais/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Simulação de Acoplamento Molecular , Trametes/enzimologia , Xilenos/análise
9.
Molecules ; 24(4)2019 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-30781874

RESUMO

Searching for the new anticancer compounds we prepared three new ß-cyclocitral-derived hydroxyl-γ-lactones by microbial hydroxylation of tetramethyl-substituted bicyclic γ-lactone. The substrate was transformed by the enzymatic system of filamentous fungi. Three out of fifteen strains were selected as effective biocatalysts (Fusarium culmorum AM10, Armillaria mellea AM296, Trametes versicolor AM536). The hydroxylation processes were not only regioselective but also stereoselective. The hydroxylation products of each secondary carbon atom in the cyclohexane ring were obtained by the application of the selected fungal strains. The Fusarium culmorum AM10 introduced the hydroxy function at C-3 and C-4, Armillaria mellea AM296 incorporated the hydroxy function at C-3 and C-5 and Trametes versicolor AM536 transformed the substrate to the mixture of C-3, C-4 and C-5 hydroxylactones. The hydroxylactones obtained were enantiomericaly enriched (ee values in the range 17⁻99%). The in vitro antiproliferative activities of the functionalization products were also evaluated. Regardless of the hydroxy substituent location all tested lactones exhibited similar, significant activity towards selected cancer cell lines (IC50 in the range 22.8⁻33.9 µg/mL).


Assuntos
Aldeídos/química , Antineoplásicos/química , Diterpenos/química , Lactonas/química , Antineoplásicos/síntese química , Antineoplásicos/farmacologia , Armillaria/química , Armillaria/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Fusarium/metabolismo , Humanos , Radical Hidroxila/química , Hidroxilação , Lactonas/síntese química , Lactonas/farmacologia , Neoplasias/tratamento farmacológico , Especificidade por Substrato , Trametes/química , Trametes/metabolismo
10.
N Biotechnol ; 50: 44-51, 2019 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-30668986

RESUMO

The high-molecular weight fraction of olive mill wastewater (HMW-OMW), a byproduct of olive oil biorefinery, was used at the reactor level as the basal medium for production of laccase and Mn-dependent peroxidase (MnP) by Trametes ochracea. Three reactor systems, namely stirred tank reactors equipped with either Rushton turbines or marine impeller and draft tube (STR and STR-MD, respectively) and an air-lift reactor (ALR) were compared for this purpose. Although inocula were supplied as intact pellets, in both STR-based systems fungal growth evolved rapidly into a dispersed form while the ALR enabled the maintenance of the pellet growth mode. STR was deemed to be the most promising system since it best supported the production MnP activity on the HMW-OMW-based medium and its performance in laccase production did not differ from that observed with the STR-MD. Among the stirring regimes considered (250, 400, 500 and 600 rpm), the best production in the STR was observed at 500 rpm and 1.0 vvm for both laccase (8850 ± 270 IU L-1 on day 15) and MnP (17,027.4 ± 87.2 IU L-1 on day 13). When the inocula were supplied to the STR in homogenized form, the MnP production peak (16,856 ± 1070 IU L-1) was attained 8 days earlier than the previous condition and that of laccase was nearly doubled (14,967 ± 907 IU L-1). When compared with literature data, T. ochracea MnP production and productivity on the HMW-OMW-based medium were the highest reported for a wild-type fungal strain.


Assuntos
Lacase/biossíntese , Lignina/metabolismo , Azeite de Oliva/metabolismo , Peroxidases/biossíntese , Trametes/metabolismo , Eliminação de Resíduos Líquidos , Águas Residuárias/química , Peso Molecular , Azeite de Oliva/química , Trametes/enzimologia
11.
Colloids Surf B Biointerfaces ; 175: 671-679, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30590328

RESUMO

In this research we report the biological synthesis of electrically conducting polymer - Polypyrrole (Ppy). Cell-assisted enzymatic polymerization/oligomerization of Ppy was achieved using whole cell culture and cell-free crude enzyme extract from two white-rot fungal cultures. The selected fungal strains belong to Trametes spp., known laccase producers, commonly applied in bioremediation and bioelectrochemical fields. The biocatalytic reaction was initiated in situ through the copper-containing enzymes biosynthesized within the cell cultures under submerged aerobe cultivation conditions. The procedure was inspired by successful reports of laccase-catalyzed pyrrole polymerization. The usage of whole culture and/or crude enzyme extract has the advantage of overcoming enzyme purification and minimizing the liability of enzyme inactivation through improved stability of enzymes in their natural environment. Spectral and electrochemical techniques (UV-vis spectroscopy, infrared spectroscopy; cyclic voltammetry (CV)) and pH measurements provided insight into the evolution of pyrrole polymerization/oligomerization and the electrochemical features of the final product. Microscopy techniques (optical microscopy and scanning electron microscopy (SEM)) were primary tools for visualization of the formed Ppy particles. The relevance of our research is twofold: Ppy prepared in crude enzyme extract results in enzyme encapsulated within Ppy and/or Ppy-modified fungal cells can be formed when polymerization occurs in whole cell culture. The route of biocatalysis can be chosen according to the desired bioelectrochemical application. The reported study focuses on the improvement of charge transfer through the fungal cell membrane and/or cell wall by modification of the fungal cells with conducting polymer - polypyrrole.


Assuntos
Proteínas Fúngicas/metabolismo , Monofenol Mono-Oxigenase/metabolismo , Polímeros/metabolismo , Pirróis/metabolismo , Trametes/metabolismo , Benzotiazóis/metabolismo , Benzotiazóis/farmacologia , Biocatálise , Parede Celular/efeitos dos fármacos , Parede Celular/metabolismo , Meios de Cultura/química , Meios de Cultura/farmacologia , Condutividade Elétrica , Técnicas Eletroquímicas , Fermentação/efeitos dos fármacos , Guaiacol/metabolismo , Guaiacol/farmacologia , Hidrazonas/metabolismo , Hidrazonas/farmacologia , Concentração de Íons de Hidrogênio , Polímeros/química , Pirróis/química , Ácidos Sulfônicos/metabolismo , Ácidos Sulfônicos/farmacologia , Trametes/efeitos dos fármacos
12.
Chemosphere ; 215: 535-542, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30340161

RESUMO

Non-steroidal anti-inflammatory drugs (NSAIDs) are widely found pollutants in the aquatic environment and the currently available treatments for their removal are usually associated with some drawbacks. The aim of this research was to apply a laccase-mediator system for the degradation of some commonly used NSAIDs, namely diclofenac (DCF), naproxen (NAP) and ketoprofen (KP). The biocatalyst was obtained by direct immobilization on chitosan beads of a periodate-oxided laccase from Trametes versicolor. A preliminary study aimed to optimize DCF degradation in the presence of 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonicacid) diammonium salt (ABTS) as mediator. It turned out that pH 3 and a 1:1 M ratio for ABTS:drug were the best experimental conditions under which DCF was degraded at 90% after 3 h. In addition, an efficient reuse of the biocatalyst for up to 5 cycles emerged. DCF was further mixed with naproxen and ketoprofen to test whether laccase was still able to eliminate DCF and eventually act on the other compounds. At just 0.02 U of laccase activity, diclofenac was completely degraded within 3 h, while an almost complete removal for naproxen (∼90%) and a partial removal for ketoprofen (30%) occurred in 7 d when drugs were added at high concentrations (78.5 µM, 98 µM and 108 µM, respectively). After 7 d of degradation, transformation products of diclofenac, identified as hydroxylated compounds, disappeared. Naproxen products were, instead, reduced to very small amounts.


Assuntos
Anti-Inflamatórios não Esteroides/química , Biodegradação Ambiental , Lacase/química , Diclofenaco/química , Hidroxilação , Cetoprofeno/química , Naproxeno/química , Trametes/metabolismo
13.
Int J Biol Macromol ; 125: 514-525, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30528991

RESUMO

A new manganese peroxidase-producing white-rot basidiomycete fungus was isolated from symptomatic wood of the camphor trees Cinnamomum camphora (L.) at the Hamma Botanical Garden (Algeria) and identified as Trametes pubescens strain i8. The enzyme was purified (MnP TP55) to apparent electrophoretic homogeneity and biochemically characterized. The specific activity and Reinheitzahl value of the purified enzyme were 221 U/mg and 2.25, respectively. MALDI-TOF/MS analysis revealed that the purified enzyme was a monomer with a molecular mass of 55.2 kDa. The NH2-terminal sequence of the first 26 amino acid residues of MnP TP55 showed high similarity with those of white-rot fungal peroxidases. It revealed optimal activity at pH 5 and 40 °C. This peroxidase was completely inhibited by sodium azide and potassium cyanide, suggesting the presence of heme-components in its tertiary structure. Interestingly, MnP TP55 showed higher catalytic efficiency, organic solvent-tolerance, dye-decolorization ability, and detergent-compatibility than that of horseradish peroxidase (HRP) from roots of Armoracia rustanica, manganese peroxidase from Bjerkandera adusta strain CX-9 (MnP BA30), and manganese peroxidase from Phanerochaete chrysosporium (MnP PC). Overall, the findings provide strong support for the potential candidacy of MnP TP55 for environmental applications, mainly the development of enzyme-based technologies for lignin biodegradation, textile-dyes biodecolorization, and detergent formulations.


Assuntos
Coriolaceae/enzimologia , Fungos/enzimologia , Lignina/metabolismo , Peroxidases/metabolismo , Trametes/metabolismo , Argélia , Aminoácidos/metabolismo , Biodegradação Ambiental , Catálise , Corantes/metabolismo , Coriolaceae/metabolismo , Fungos/metabolismo , Peroxidase do Rábano Silvestre/metabolismo , Phanerochaete/metabolismo , Têxteis
14.
Environ Pollut ; 246: 346-356, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30577003

RESUMO

Antidepressant drugs such as Venlafaxine (VFX) and O-desmethylvenlafaxine (ODMVFX) are emerging contaminants that are commonly detected in aquatic environments, since conventional wastewater treatment plants are unable to completely remove them. They can be precursors of hazardous by-products, such as the carcinogenic N-nitrosodimethylamine (NDMA), generated upon water chlorination, as they contain the dimethylamino moiety, necessary for the formation of NDMA. In this study, the capability of three white rot fungi (Trametes versicolor, Ganoderma lucidum and Pleurotus ostreatus) to remove both antidepressants from water and to decrease NDMA formation potential was investigated. Furthermore, transformation by-products (TPs) generated along the treatment process were elucidated and also correlated with their NDMA formation potential. Very promising results were obtained for T. versicolor and G. lucidum, both being able to remove up to 100% of ODMVFX. In the case of VFX, which is very recalcitrant to conventional wastewater treatment, a 70% of removal was achieved by T. versicolor, along with a reduction in NDMA formation potential, thus decreasing the associated problems for human health and the environment. However, the NDMA formation potential remained practically constant during treatment with G. lucidum despite of the equally high VFX removal (70%). This difference was attributed to the generation of different TPs during both fungal treatments. For example, G. lucidum generated more ODMVFX, which actually has a higher NDMA formation potential than the parent compound itself.


Assuntos
Succinato de Desvenlafaxina/metabolismo , Dimetilnitrosamina/metabolismo , Trametes/metabolismo , Cloridrato de Venlafaxina/metabolismo , Águas Residuárias/análise , Poluentes Químicos da Água/metabolismo , Purificação da Água/métodos , Biodegradação Ambiental , Águas Residuárias/microbiologia , Poluentes Químicos da Água/análise
15.
J Biotechnol ; 284: 52-56, 2018 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-30107199

RESUMO

Quantum dots are important fluorescent semiconductor nano-crystals with distinguished electrical and optical properties and have gained great interest in many fields. The chemical and physical synthetic methods are usually not favorable for biological application due to high energy-consumption procedure and residual toxic chemicals. The development of novel "green" routes to prepare bio-compatible cadmium sulfide quantum dots constitutes a promising substituted approach. We used the white rot fungus Trametes versicolor for the biosynthesis of cadmium sulfide quantum dots taking account of the adsorption property of this fungus. Multiple physical characterizations involving scanning electron microscope (SEM), ultraviolet-visible (UV-vis) and photoluminescence (PL) spectroscopy, fourier transform infrared spectroscopy (FTIR), thermo-gravimetric (TG), transmission electron microscopy (TEM) and X-ray diffraction (XRD) confirmed surface, optical and thermal characteristics, crystalline nature, size and shape distributions of the nanoparticles. This study provided a suitable and efficient approach to synthesize stable biocompatible cadmium sulfide quantum dots using the fungus Trametes versicolor with great potentials in the biological and biomedical researches.


Assuntos
Compostos de Cádmio/metabolismo , Pontos Quânticos/metabolismo , Sulfetos/metabolismo , Trametes/metabolismo , Microbiologia Industrial
16.
Ecotoxicol Environ Saf ; 164: 425-433, 2018 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-30144702

RESUMO

In the bioethanol industry and its derivative processes, 9-14 l of vinasse per liter of ethanol are obtained as residue, depending on whether or not a recirculation process is used. If the vinasse is discharged directly into water bodies without proper treatment it can produce negative effects on existing biota and on human health. The main objective of this study was to evaluate the capacity of a mixed treatment with Komagataeibacter kakiaceti GM5 and Trametes versicolor DSM 3086 to degrade and reduce the toxicity of this by-product. In order to carry out the tests, an experimental culture media of vinasse at 60% (v/v) was used. This culture was compared to a standard medium of glucose at 2% and, simultaneously, a mixed culture (bacterium-fungus) was used through a batch process in an orbital shaker. When compared to the treatments evaluated with individually grown microorganisms, the mixed treatment proposed produced the best COD results in vinasse degradation. However, better results were obtained when using the treatment with T. versicolor DSM 3086 for color removal and treatment with K. kakiaceti GM5 produced better results in terms of turbidity and toxicity. Although the simultaneous mixed treatment did not improve all the variables studied, it never showed the lowest performance. Therefore, this proposal can be considered as an innovative biological alternative in degradation processes of great organic pollutants such as vinasse.


Assuntos
Acetobacteraceae/metabolismo , Biodegradação Ambiental , Trametes/metabolismo , Animais , Biocombustíveis , Análise da Demanda Biológica de Oxigênio , Biomassa , Fenômenos Químicos , Meios de Cultura/química , Daphnia/metabolismo , Etanol , Humanos , Concentração de Íons de Hidrogênio , Açúcares/química , Testes de Toxicidade Aguda
17.
PLoS One ; 13(7): e0201131, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30024975

RESUMO

Polysaccharides derived from mushrooms have potential to control blood sugar, reduce insulin resistance and prevent diabetic complications. The intracellular polysaccharopeptides of Trametes versicolor (TV) have been used as immunologic and oncologic adjuvants. The aim of this study was to investigate the potential activities and mechanisms of extracellular polysaccharopeptides (ePSP) obtained from TV strain LH-1 on regulating glucose homeostasis. Human hepatoma HepG2 cells incubated with normal glucose (5.5 mM, NG model), high glucose (33 mM, HG model), or high glucose (33 mM) plus high insulin (10-7 M, HGI model) concentrations were administered with TV LH-1 ePSP (50, 100, and 1000 µg/ml) for 24 hr. Glucose uptake of HepG2 cells, determined by flow cytometry, was significantly decreased in the HG and HGI models with insulin stimulation, suggesting insulin resistance of these cells; however, ePSP reversed this decrease in a dose-dependent manner (one-way ANOVA, p<0.05). In the HG and HGI models, ePSP significantly increased glycogen content, insulin receptor substrate-2 protein and phosphorylated AMP-activated protein kinase (AMPK), as determined by western blot analysis. In addition, ePSP significantly increased glucokinase in the NG and HG models, increased membrane glucose transporter-1 and decreased glycogen synthase kinase-3ß in the HGI model, and increased glucose-6-phosphatase in the NG and HGI models (one-way ANOVA, p<0.05). In summary, TV LH-1 ePSP may elevate cellular glucose uptake to regulate glucose homeostasis via the activation of AMPK and glycogen synthesis in an insulin-independent manner. These results suggest that TV LH-1 ePSP may be a nutraceutical with anti-hyperglycemic activity.


Assuntos
Glucose/metabolismo , Hipoglicemiantes/farmacologia , Resistência à Insulina , Proteoglicanas/farmacologia , Trametes , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Relação Dose-Resposta a Droga , Espaço Extracelular/metabolismo , Fermentação , Regulação da Expressão Gênica/efeitos dos fármacos , Glicogênio/metabolismo , Células Hep G2 , Humanos , Insulina/administração & dosagem , Insulina/metabolismo , Proteínas Substratos do Receptor de Insulina/metabolismo , Resistência à Insulina/fisiologia , Fitoterapia , Proteoglicanas/metabolismo , Trametes/metabolismo
18.
Sci Rep ; 8(1): 8472, 2018 05 31.
Artigo em Inglês | MEDLINE | ID: mdl-29855494

RESUMO

Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level. Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones. Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site. These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation.


Assuntos
Proteínas Fúngicas/química , Glutationa Transferase/química , Desintoxicação Metabólica Fase II , Polifenóis/química , Trametes/metabolismo , Madeira/química , Sequência de Aminoácidos , Benzofenonas/química , Benzofenonas/metabolismo , Sítios de Ligação , Cristalografia por Raios X , Flavonoides/química , Flavonoides/metabolismo , Proteínas Fúngicas/metabolismo , Glutationa Transferase/metabolismo , Isoenzimas/química , Isoenzimas/metabolismo , Cinética , Polifenóis/metabolismo , Estabilidade Proteica , Estrutura Terciária de Proteína , Prunus/química , Prunus/metabolismo , Alinhamento de Sequência , Temperatura , Madeira/metabolismo
19.
Sci Rep ; 8(1): 7333, 2018 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-29743526

RESUMO

Cisplatin is a commonly used chemotherapeutic agent in the treatment of different types of malignant tumors, but nephrotoxicity limits its usage. Therefore, in this study, we aimed to determine the possible protective effect of Huaiqihuang (HQH) extractum, a kind of Chinese herbal complex that consists of Trametes robiniophila Murr., Lycium barbarum and Polygonatum sibiricum, against nephrotoxicity induced by cisplatin in mice. We found that pretreatment with HQH significantly attenuated the cisplatin-induced increase in blood urea nitrogen (BUN), interstitial congestion, acute renal tubular injury and tubular cell apoptosis and necroptosis. It was further shown that HQH administration reduced cisplatin-induced release and nuclear-cytoplasmic translocation of HMGB1 and inactivated its downstream signaling molecules, TLR4 and NFκB, in renal tubular cells; as a result, HQH repressed cisplatin-induced TNF-α production. As dexamethasone (Dex) exerts renoprotective effects in severe Acute kidney injury (AKI), we compared it with HQH and found that HQH showed similar renoprotective effects to dexamethasone via similar mechanisms. Considering the potential side effects of corticosteroids, reducing the effectiveness of treatment and shortening survival in solid tumor patients, we suggest administration of HQH as a potential adjuvant for cisplatin therapy in solid tumor patients to preserve renal function.


Assuntos
Lesão Renal Aguda/prevenção & controle , Medicamentos de Ervas Chinesas/farmacologia , Lesão Renal Aguda/patologia , Animais , Apoptose/efeitos dos fármacos , Nitrogênio da Ureia Sanguínea , China , Cisplatino/efeitos adversos , Cisplatino/farmacologia , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/metabolismo , Proteína HMGB1/metabolismo , Rim/metabolismo , Lycium/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , NF-kappa B/metabolismo , Polygonatum/metabolismo , Substâncias Protetoras/farmacologia , Transdução de Sinais/efeitos dos fármacos , Receptor 4 Toll-Like/metabolismo , Trametes/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
20.
Fungal Biol ; 122(5): 353-362, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29665961

RESUMO

Laccase (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) is an enzyme that has been studied for over 100 y and is present in virtually all fungi. As increasing numbers of fungal genomes have been sequenced, it has become apparent that the laccase genes in white-rot fungi commonly form multigene families consisting of many nonallelic genes. Although a number of reports focussing on laccase gene expression in different fungal species were published over the decades, the fundamental questions of why fungi need such a redundant array of genes and how they manage this array to perform biological function(s) remain far from answered. In this article, we present a comprehensive study of the transcription of the whole Trametes hirsuta laccase multigene family under different conditions, including exposure to different nutritional factors such as nitrogen sources (organic and inorganic) and concentrations of nitrogen and carbon in the culture medium; in different growth phases (lag phase and stationary phase); and in the presence of different inducer agents (water-soluble lignin, bromocresol green dye, p-coumaric acid, ferulic acid, guaiacol, vanillin, veratryl alcohol, vanillic acid and syringic acid). Our findings are discussed in the context of the evolution of the laccase multigene family, and the presence of transcription-level subfunctionalization is highlighted.


Assuntos
Regulação Fúngica da Expressão Gênica , Lacase/biossíntese , Trametes/enzimologia , Trametes/genética , Transcrição Genética , Carbono/metabolismo , Meios de Cultura/química , Ativadores de Enzimas/metabolismo , Lacase/genética , Família Multigênica , Compostos de Nitrogênio/metabolismo , Trametes/crescimento & desenvolvimento , Trametes/metabolismo
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