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1.
Methods Mol Biol ; 2218: 37-47, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33606221

RESUMO

Cryopreservation as a method that enables long-term storage of biological material has long been used for the conservation of valuable zebrafish genetic resources. However, currently, only spermatozoa of zebrafish can be successfully cryopreserved, while protocols for cryopreservation of eggs and embryos have not yet been fully developed. Transplantation of germline stem cells (GSCs) has risen as a favorable method that can bypass the current problem in cryopreservation of female genetic resources and can lead to reconstitution of fish species and lines through surrogate production. Here, we describe essential steps needed for the cryopreservation of spermatogonial stem cells (SSCs) and their utilization in the conservation of zebrafish genetic resources through SSC transplantation and surrogate production.


Assuntos
Células-Tronco Germinativas Adultas/citologia , Criopreservação/métodos , Espermatogônias/citologia , Espermatozoides/citologia , Transplante/métodos , Células-Tronco Germinativas Adultas/efeitos dos fármacos , Animais , Crioprotetores/farmacologia , Masculino , Espermatogônias/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Testículo/citologia , Testículo/efeitos dos fármacos , Peixe-Zebra/fisiologia
2.
Carbohydr Polym ; 254: 117296, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33357864

RESUMO

A fully biobased benzoxazine monomer, V-fa (using vanillin and furfurylamine) was grafted onto chitosan (CS) at different weight ratios (CXVY) using "grafting to" benign Schiff base chemistry. Incorporation of V-fa onto CS increased the tensile strength and improved chemical resistance of the CS-graft-V-fa films. Reversible labile linkages, expansion of CS galleries and leaching out of phenolic species from biobased polymer films led to an improved antibacterial activity against Staphylococcus aureus, which is ∼125 times higher than the bare CS film, V-fa and oligomeric V-fa. The leached out species from films were analyzed extensively by NMR, FTIR, GPC, ABTS and HRMS analysis. Oxidative-stress seems to be responsible for antibacterial activity. Current work illustrates an attractive synthetic approach and the improved antibacterial performance of biobased CS-graft-poly(V-fa) films which may hold as a potential alternative for wound-healing and implant applications in future.


Assuntos
Antibacterianos/química , Antioxidantes/química , Benzoxazinas/química , Quitosana/química , Liberação Controlada de Fármacos , Staphylococcus aureus/efeitos dos fármacos , Benzaldeídos/química , Furanos/química , Interações Hidrofóbicas e Hidrofílicas , Viabilidade Microbiana/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacos , Solventes/química , Staphylococcus aureus/metabolismo , Resistência à Tração , Transplante/métodos , Água/química , Cicatrização/efeitos dos fármacos
3.
Methods Mol Biol ; 2191: 235-259, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32865749

RESUMO

This paper describes research methods to investigate the development of synaptic connections between transplanted GABAergic interneurons and endogenous neurons in the adult mouse hippocampus. Our protocol highlights methods for retroviral labeling adult-born GCs, one of the few cell types in the adult brain to be continuously renewed throughout life. By precise targeting of the retrovirus, labeling of adult-born GCs can be combined with optogenetic stimulation of the transplanted cells and electrophysiology in brain slices, to test whether the GABAergic interneurons integrate and establish inhibitory synaptic connections with host brain neurons. Modifications to adult neurogenesis are an important contributing factor in the development and severity of TLE and seizures. When combined with retroviral labeling, the approaches we describe in this chapter can be used to determine whether transplantation modifies the process of adult neurogenesis or other properties of the hippocampus. These approaches are helping to define parameters for potential cell replacement therapies to be used in patients with intractable seizure disorders.


Assuntos
Neurônios GABAérgicos/metabolismo , Hipocampo/metabolismo , Interneurônios/metabolismo , Optogenética/métodos , Animais , Hipocampo/patologia , Camundongos , Transplante/métodos
4.
Texto & contexto enferm ; 29: e20180310, Jan.-Dec. 2020.
Artigo em Inglês | LILACS, BDENF - Enfermagem | ID: biblio-1101979

RESUMO

ABSTRACT Objective: to analyze adherence to the nursing guidelines for home care of bone marrow transplant recipients from an ecosystem perspective. Method: descriptive, exploratory study with a qualitative approach, using Content Analysis for data analysis, with theoretical and philosophical ecosystem support. The interviews, carried out in bone marrow transplant services, in Brazil and Spain, were guided by an instrument developed by the researchers which contained 25 closed and ten open questions. 40 users participated who met the inclusion criteria. Data collection was carried out from July 2016 to October 2017. Results: the Orientations category emerged from the data which then gave rise to the subcategories: Interactive relational actions; and, actions and behaviors that interfered in the success of the transplant. Some users, due to excessive information at the time of discharge, were unable to assimilate or carry out the guidelines received; others, during the hospitalization phase, apprehended them and absorbed them in order to use them in the home ecosystem space after transplantation. Conclusion: part of the users followed only the guidelines that best adapted to their daily lives and, for others, after hospital discharge, they caused doubts and insecurities regarding the care to be performed at home. It is necessary for the user to identify the constituent elements of their home ecosystem and learn, through communication and information, how they interfere in post-hospital discharge care. Therefore, it is necessary to create communication and information mechanisms that enable the dynamic process between the constituent elements of the ecosystem, biotic and abiotic, so that they have interaction and sustainability and can be practiced by the user.


RESUMEN Objetivo: analizar el cumplimiento de las directrices de la enfermera para el cuidado domiciliario de los receptores de trasplante de médula ósea en una perspectiva ecosistémica. Método: descriptivo, exploratorio, con un enfoque cualitativo, utilizando el análisis de contenido para el análisis de datos, con soporte teórico y filosófico del ecosistema. Las entrevistas, realizadas en los servicios de trasplante de médula ósea, en Brasil y España, fueron guiadas por un instrumento desarrollado por los investigadores, que contenía 25 preguntas cerradas y diez abiertas. Participaron 40 usuarios que cumplieron con los criterios de inclusión. La recolección de datos se realizó entre julio de 2016 y octubre de 2017. Resultados: la categoría Orientaciones surgió de los datos y originó las subcategorías: acciones relacionales interactivas, acciones y comportamientos que interfirieron en el éxito del trasplante. Algunos usuarios, debido a la información excesiva al momento del alta, no pudieron asimilar y llevar a cabo las pautas recibidas, otros, en el curso de la fase de hospitalización, los detuvieron para absorberlos para su atención en el espacio del ecosistema del hogar en el pos trasplante. Conclusión: parte de lós usuários siguió solo las pautas que mejor se adaptaron a su vida diaria y para otros, después del alta hospitalaria, causaron dudas e inseguridades com respecto a La atención que se practica em elhogar. Es necesario que el usuario identifique los elementos constitutivos del ecosistema de su hogar y aprenda, a través de la comunicación y la información, cómo interfieren en la atención hospitalaria posterior al alta. Por lo tanto, es necesario crear mecanismos de comunicación e información que permitan el proceso dinámico entre los elementos constitutivos del ecosistema, bióticos y abióticos, para que tengan interacción y sostenibilidad y puedan ser practicados por el usuario.


RESUMO Objetivo: analisar a adesão às orientações do enfermeiro para o cuidado domiciliar do transplantado de medula óssea na perspectiva ecossistêmica. Método: descritivo, exploratório, com abordagem qualitativa, utilizando-se para a análise dos dados a Análise de Conteúdo, com apoio teórico-filosófico ecossistêmico. As entrevistas, realizadas em serviços de transplante de medula óssea, no Brasil e Espanha, foram norteadas por um instrumento elaborado pelas pesquisadoras, contendo 25 questões fechadas e dez abertas. Participaram 40 usuários que cumpriram os critérios de inclusão. A coleta de dados foi realizada de julho de 2016 a outubro de 2017. Resultados: a categoria Orientações emergiu dos dados e originou as subcategorias: Ações relacionais interativas; e, ações e comportamentos que interferiram no sucesso do transplante. Alguns usuários, por excesso de informações no momento da alta, não conseguiram assimilar e desempenhar as orientações recebidas; outros, no transcorrer da fase de internação, as apreenderam absorvê-las para o cuidado no espaço ecossistêmico domiciliar no pós-transplante. Conclusão: parte dos usuários seguiu somente as orientações que melhor se adaptaram ao seu cotidiano e, para outros, no pós-alta hospitalar, ocasionaram dúvidas e inseguranças em relação ao cuidado a ser praticado no domicílio. Faz-se necessário que o usuário identifique os elementos constituintes do seu ecossistema domiciliar e conheça, por meio da comunicação e informação, como interferem no cuidado pós alta hospitalar. Portanto, é preciso criar mecanismos de comunicação e informação que possibilitem o processo dinâmico entre os elementos constituintes do ecossistema, bióticos e abióticos, para que tenham interação e sustentabilidade e possam ser praticados pelo usuário.


Assuntos
Humanos , Adulto , Adulto Jovem , Transplante , Medula Óssea , Transplante de Medula Óssea , Transplantes , Cuidados de Enfermagem , Enfermagem , Ecossistema , Transplantados , Cooperação e Adesão ao Tratamento , Habitação
5.
Rev. SOBECC ; 25(4): 219-226, 21-12-2020.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1141399

RESUMO

Objetivo: Conhecer a percepção dos enfermeiros que atuam em uma unidade de transplantes sobre os desafios de sua atuação ante a COVID-19. Método: Estudo descritivo e exploratório, com abordagem qualitativa, desenvolvido em uma unidade de transplantes de um hospital referência do Ceará, entre março e junho de 2020, por meio de entrevista. Os dados foram analisados pelo software Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires (IRAMUTEQ). Posteriormente, foi realizada a análise fatorial por correspondência (AFC) e gerada uma nuvem de palavras para a qual foram consideradas as evocações que apareceram com maior frequência. Resultados: Participaram do estudo 14 enfermeiros, a maioria do sexo feminino. O conteúdo analisado foi categorizado em três classes: organização do fluxo de atendimento de casos suspeitos, equipamento de proteção individual no contexto da pandemia da COVID-19, o emocional ante a pandemia. Conclusão: Constatou-se que os enfermeiros sofrem ante as fragilidades relacionadas ao momento vivenciado, pois estão sendo cotidianamente desafiados a se adaptarem às mudanças em suas rotinas de trabalho.


Objective: To understand how nurses who work in a transplant unit perceive the challenges of their role in the face of COVID-19. Method: This is an exploratory, descriptive, qualitative study conducted through interviews in the transplant unit of a reference hospital in Ceará, Northeastern Brazil, between March and June 2020. Data were analyzed in the software Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires (IRAMUTEQ). Subsequently, we performed a correspondence factor analysis (CFA) and generated a word cloud, taking into account the evocations with higher frequency. Results: Fourteen nurses participated in the study, most of them women. The content analyzed was categorized into three classes: organization of the care flow for suspected cases, personal protection equipment in the COVID-19 pandemic context, emotional state in the face of the pandemic. Conclusion: We found that nurses are suffering due to fragilities related to the moment they are experiencing, as they are daily challenged to adapt to changes in their work routines.


Objetivo: Conocer la percepción de los enfermeros que laboran en una unidad de trasplante sobre los desafíos de su desempeño ante el COVID- 19. Método: Estudio descriptivo y exploratorio, con abordaje cualitativo, desarrollado en una unidad de trasplante de un hospital de referencia en Ceará, entre marzo y junio de 2020, mediante entrevista. Los datos fueron analizados por el software IRAMUTEQ (Interface de R pour les Analyses Multimensionnelles de Textes et de Questionnaires). Posteriormente, se realizó el Análisis Factorial por Correspondencia (AFC) y se generó un Word Cloud para el que se consideraron las evocaciones que aparecían con mayor frecuencia. Resultados: participaron del estudio 14 enfermeras, la mayoría mujeres. El contenido analizado se categorizó en tres clases: Organización del Flujo de Servicios de Casos Sospechosos, Equipo de Protección Personal en el Contexto de la Pandemia de COVID-19, La Emocional Frente a la Pandemia. Conclusión: Se encontró que los enfermeros padecen las debilidades relacionadas con el momento vivido, ya que diariamente se enfrentan al desafío de adaptarse a los cambios en sus rutinas laborales.


Assuntos
Humanos , Masculino , Feminino , Percepção , Conhecimentos, Atitudes e Prática em Saúde , Infecções por Coronavirus/enfermagem , Pandemias , Betacoronavirus , Profissionais de Enfermagem , Pneumonia Viral/enfermagem , Transplante/enfermagem , Emoções , Equipamento de Proteção Individual , Profissionais de Enfermagem/psicologia , Cuidados de Enfermagem
6.
Cell Tissue Bank ; 21(4): 557-562, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33063150

RESUMO

On March 19 World Health Organization declare the pandemic situation by outbreak coronavirus disease 2019 in the world. The pressure on the health care system has been very high in several countries. Spanish National Transplant Organization (ONT) have made many efforts in maintaining transplantation activity. Although the impact of the pandemic on organ activity has been analysed, to date, less data exist regarding the impact on tissue activity. The aim of this study has been the evaluation of the possible impact on the procurement, processing and distribution of tissues during the peak period of the pandemic COVID-19 in Spain. For this study, a multicentre analysis has been made with a survey of the tissue banks in Spain, during the period March 1 to April 30, 2020. Our data suggest that the impact of coronavirus in Spain has affected dramatically tissue donation but with a moderate effect on stored tissues such as bone, valves, vessels or skin. Tissue banks should prepare if future next pandemic waves surges so that tissue provision is guaranteed both in urgent and elective surgeries.


Assuntos
Infecções por Coronavirus/epidemiologia , Pneumonia Viral/epidemiologia , Bancos de Tecidos/estatística & dados numéricos , Obtenção de Tecidos e Órgãos/estatística & dados numéricos , Transplante/estatística & dados numéricos , Betacoronavirus , Humanos , Pandemias , Espanha/epidemiologia , Inquéritos e Questionários
8.
Rev. am. med. respir ; 20(3): 195-196, sept. 2020.
Artigo em Espanhol | LILACS, BINACIS | ID: biblio-1123016

RESUMO

El trasplante pulmonar es una alternativa válida para el tratamiento de pacientes adecuadamente seleccionados con enfermedades pulmonares en etapa terminal. El proceso quirúrgico del trasplante, si bien es complejo, puede resumirse mencionando que comienza con la obtención de un órgano adecuado para implantar y finaliza con el implante en sí mismo. El concepto de qué es un órgano adecuado fue y es objeto de numerosos estudios y sus respectivas publicaciones. La escasez de órganos adecuados para trasplante es el principal determinante que limita el trasplante pulmonar en todo el mundo. Numerosos criterios han sido postulados y se utilizan para evaluar un potencial injerto: uno de los criterios aceptados universalmente es que el mismo no se encuentre infectado.


Assuntos
Humanos , Pneumopatias , Transplante , Infecções por Coronavirus , Síndrome Respiratória Aguda Grave , Pulmão
10.
Nat Commun ; 11(1): 2758, 2020 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-32488069

RESUMO

Human beige adipocytes (BAs) have potential utility for the development of therapeutics to treat diabetes and obesity-associated diseases. Although several reports have described the generation of beige adipocytes in vitro, their potential utility in cell therapy and drug discovery has not been reported. Here, we describe the generation of BAs from human adipose-derived stem/stromal cells (ADSCs) in serum-free medium with efficiencies >90%. Molecular profiling of beige adipocytes shows them to be similar to primary BAs isolated from human tissue. In vitro, beige adipocytes exhibit uncoupled mitochondrial respiration and cAMP-induced lipolytic activity. Following transplantation, BAs increase whole-body energy expenditure and oxygen consumption, while reducing body-weight in recipient mice. Finally, we show the therapeutic utility of BAs in a platform for high-throughput drug screening (HTS). These findings demonstrate the potential utility of BAs as a cell therapeutic and as a tool for the identification of drugs to treat metabolic diseases.


Assuntos
Adipócitos Bege/metabolismo , Terapia Baseada em Transplante de Células e Tecidos/métodos , Descoberta de Drogas/métodos , Doenças Metabólicas/metabolismo , Adipócitos Bege/citologia , Animais , Peso Corporal , Avaliação Pré-Clínica de Medicamentos , Metabolismo Energético , Feminino , Ensaios de Triagem em Larga Escala , Humanos , Masculino , Células-Tronco Mesenquimais , Doenças Metabólicas/tratamento farmacológico , Camundongos , Camundongos Endogâmicos NOD , Camundongos SCID , Mitocôndrias/metabolismo , Consumo de Oxigênio , Células Estromais , Transplante
12.
Transplant Proc ; 52(9): 2596-2600, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32471628

RESUMO

Background: Although hospital systems have largely halted elective surgical practices in preparing their response to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, transplantation remains an essential and lifesaving surgical practice. To continue transplantation while protecting immunocompromised patients and health care workers, significant restructuring of normal patient care practice habits is required. Methods: This is a nonrandomized, descriptive study of the abdominal transplant program at 1 academic center (University of California, San Francisco) and the programmatic changes undertaken to safely continue transplantations. Patient transfers, fellow use, and patient discharge education were identified as key areas requiring significant reorganization. Results: The University of California, San Francisco abdominal transplant program took an early and aggressive approach to restructuring inpatient workflows and health care worker staffing. The authors formalized a coronavirus disease 2019 (COVID-19) transfer system to address patients in need of services at their institution while minimizing the risk of SARS-CoV-2 in their transplant ward and used technological approaches to provide virtual telehealth where possible. They also modified their transplant fellow staffing and responsibilities to develop an adequate backup system in case of potential exposures. Conclusion: Every transplant program is unique, and an individualized plan to adapt and modify standard clinical practices will be required to continue providing essential transplantation services. The authors' experience highlights areas of attention specific to transplant programs and may provide generalizable solutions to support continued transplantation in the COVID-19 era.


Assuntos
Infecções por Coronavirus , Pandemias , Pneumonia Viral , Transplante/normas , Fluxo de Trabalho , Betacoronavirus , Humanos , Assistência ao Paciente/métodos , Assistência ao Paciente/normas , São Francisco , Transplante/métodos
13.
Hematol Oncol Stem Cell Ther ; 13(2): 58-60, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32380009

RESUMO

Despite huge progress in the fields of newborn screening, encapsulated bacterial infection prophylaxis, immunization, and supportive care in general, people suffering from sickle cell anemia still continue to have a shorter life expectancy and a poorer quality of life due to painful vaso-occlusive events and strokes during childhood, and later, cardiac, pulmonary, and renal injuries, including in Western and high-income countries. From the 2000s, allogeneic stem cell transplantation for severe sickle cell disease from a sibling donor provided the best results-overall as well as disease-free survival-never obtained for any other disease. Nevertheless, this only curative option is proposed to few patient numbers, including in Western countries with high-level medical equipment development, with discrepancies between (i) patients and family, (ii) physicians and care centers dedicated to sickle cell disease, and (iii) hematopoietic stem cell transplant teams. Due to these discrepancies and in order to provide the same quality of discussion and treatment choice for every sickle cell disease patient, we developed a National French multidisciplinary pluri-annual meeting dedicated to sickle cell disease patients and transplantation. We report here our experience of such a meeting.


Assuntos
Anemia Falciforme/terapia , Hematologia/normas , Qualidade de Vida/psicologia , Transplante/normas , Adulto , Humanos
14.
Medicine (Baltimore) ; 99(20): e20091, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32443319

RESUMO

This study aims to evaluate the changes of fragility and ultrastructure of amniotic membrane after cross-linking by UVA/riboflavin.Forty-nine fresh amniotic membranes were randomly divided into 3 groups. Eighteen were in group A (CX group) and immersed in 0.1% riboflavin solution for 10 min for UVA/riboflavin cross-linking. Sixteen were in group B (B2 group), soaked for 10 min with 0.1% riboflavin. After soaking, membranes in group A and B were transferred into corneal preservation solution. Fifteen pieces were in group C, directly into corneal preservation solution. The biomechanical and ultrastructural changes of the amniotic tissue before and after cross-linking were examined (CX group = 13, B2 group = 11, C group = 15). The amniotic membrane tissue of group A (n = 5) and B (n = 5) was transplanted into 16 eyes of the rabbits, respectively, and the dissolution time of the amniotic membrane tissue was investigated.After cross-linking, compared with the control group, the elastic modulus of the low-stress area of the amniotic membrane (Elow) was higher, while the elastic modulus of the high-stress area of the amniotic membrane (Ehigh) was lower, with no significant difference in the tensile strength. Also, the collagen fibers showed coarse and bamboo-like changes. In group A, amniotic membranes began to dissolve 4 weeks after conjunctiva transplantation, and all amniotic membranes were dissolved and absorbed 6 weeks after conjunctiva transplantation. In group B, some amniotic membrane tissues were still visible 6 weeks after conjunctiva transplantation.This study suggested that after amniotic membrane cross-linking, the brittleness was increased, the hardness was enhanced, and the morphology of the collagen fiber was changed. The cross-linked amniotic membrane showed resistance to tissue dissolution.


Assuntos
Âmnio/fisiologia , Âmnio/ultraestrutura , Reagentes para Ligações Cruzadas , Riboflavina , Transplante , Raios Ultravioleta , Implantes Absorvíveis , Âmnio/efeitos dos fármacos , Âmnio/transplante , Animais , Colágeno/efeitos dos fármacos , Colágeno/efeitos da radiação , Módulo de Elasticidade , Olho , Humanos , Procedimentos Cirúrgicos Oftalmológicos , Soluções para Preservação de Órgãos , Coelhos , Distribuição Aleatória
15.
Bone Marrow Transplant ; 55(10): 1900-1905, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32313181

RESUMO

Italy is the second exposed country worldwide, after China, and Lombardia is the most affected region in Italy, with more than half of the national cases, with 13% of whom being healthcare professionals. The Clinica Pediatrica Università degli Studi di Milano Bicocca is a general pediatric and hematology oncology and transplant center embedded within the designated COVID-19 general Hospital San Gerardo in Monza, located in Lombardia, Italy. Preventive and control measures specifically undertaken to cope with the emergency within hemato-oncology, transplant, and outpatient unit in the pediatric department have been described. Preliminary COVID-19 experiences with the first Italian pediatric hemato-oncology patients are reported. The few available data regarding pediatrics and specifically hemato-oncological patients are discussed. The purpose of this report is to share pediatric hemato-oncology issues encountered in the first few weeks of the COVID-19 outbreak in Italy and to alert healthcare professionals worldwide to be prepared accordingly.


Assuntos
Infecções por Coronavirus/epidemiologia , Hematologia/organização & administração , Controle de Infecções/métodos , Oncologia/organização & administração , Pediatria/organização & administração , Pneumonia Viral/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Betacoronavirus , Criança , Pré-Escolar , Infecções por Coronavirus/prevenção & controle , Feminino , Hospitais , Humanos , Lactente , Recém-Nascido , Itália/epidemiologia , Masculino , Pessoa de Meia-Idade , Neoplasias/complicações , Pacientes Ambulatoriais , Pandemias/prevenção & controle , Pneumonia Viral/prevenção & controle , Transplante de Células-Tronco , Transplante , Adulto Jovem
16.
Sports Med Arthrosc Rev ; 28(2): 41-48, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32345925

RESUMO

Surgical reconstruction of the anterior cruciate ligament (ACL) is often indicated to restore functional stability and prevent early degeneration of the knee joint, as there is little biological healing capacity of the native ACL. Although a reconstructed ACL does not fully restore the original structure or biomechanics properties of the native ACL, the graft used for reconstruction must not only have structural and mechanical properties that closely resemble those of the native ligament, it must also have minimal antigenicity and enough biological potential to incorporate into host bone. There are several considerations in graft selection: autograft versus allograft, and soft tissue grafts versus grafts with bone plugs. Commonly used grafts include bone-patella tendon-bone, hamstring, and quadriceps; among allografts, options further include tibias anterior and posterior, Achilles, an peroneal tendons. Optimal graft selection is not only dependent on graft properties, but perhaps more importantly on patient characteristics and expectations. The purpose of this review is to summarize the relevant biological, biomechancial, and clinical data regarding various graft types and to provide a basic framework for graft selection in ACL reconstruction.


Assuntos
Reconstrução do Ligamento Cruzado Anterior/métodos , Tendões/transplante , Transplante/métodos , Transplantes/classificação , Humanos , Volta ao Esporte
17.
Sci Rep ; 10(1): 3979, 2020 03 04.
Artigo em Inglês | MEDLINE | ID: mdl-32132617

RESUMO

The spleen is a large lymphoid organ located in the abdomen that filters blood and regulates the immune system. The extent of mobilization of splenic immune cells to peripheral tissues in health and disease, however, remains poorly understood. This is due, in large part, to a lack of in vivo, spleen-specific lineage tagging strategies. Here, we describe a detailed practical protocol of spleen transplantation and its evaluation for long-term graft survival. Unlike implantation of splenic morsels in the great omentum, our approach uses arterial and venous anastomoses which rapidly restores blood flow and facilitates long-term survival of the graft. The use of congenic mouse strains permits the use of immunofluorescence and flow cytometry-based methodologies to unambiguously track the migration of spleen-derived cells to peripheral tissues.


Assuntos
Anastomose Cirúrgica , Artérias/cirurgia , Baço/cirurgia , Transplante/métodos , Veias/cirurgia , Animais , Sobrevivência de Enxerto , Camundongos , Modelos Animais
18.
Hematology ; 25(1): 125-133, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32153257

RESUMO

Objectives: T-cell immunoglobulin and mucin domain-containing protein-3 (TIM-3) is considered as a negative regulator of T-cell driven immune response. This study is planned to investigate the prognostic role of pre-transplant soluble TIM-3 (sTIM-3) levels in allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. Methods: Pre-transplant serum sTIM-3 levels were measured in 177 allo-HSCT recipients [median age: 36(16-66) years; male/female: 111/66]. Results: Pre-transplant sTIM-3 levels were significantly higher in acute myeloid leukemia (AML) patients compared to acute lymphoblastic leukemia (ALL) patients (p = 0.01). Pre-transplant sTIM-3 levels were significantly lower in patients with abnormal cytogenetics (p = 0.017). Pre-transplant sTIM-3 levels were significantly higher in patients who developed viral hemorrhagic cystitis (p = 0.034). A positive correlation was demonstrated between sTIM-3 levels and acute graft versus host disease (GvHD) grade (p = 0.013; r = 0.299). Overall survival (OS) was not statistically different between low- and high-TIM-3 groups (%35.2 vs %20.4; p > 0.05). Primary diagnosis (p = 0.042), sinusoidal obstruction syndrome (p < 0.001), acute GvHD (p = 0.001), chronic GvHD (p = 0.009) and post-transplant relapse (p = 0.003) represented significant impact on OS. Discussion: Increased sTIM-3 levels in AML patients seem to be compatible with the previous reports. The inhibitor role of TIM-3 in cellular immune response may be a possible explanation for the association of sTIM-3 with viral infections and GvHD. However, the main challenge remains to be the ambiguous association of pre-transplant sTIM-3 levels and post-transplant complications, as allo-HSCT recipients are expected to represent donor genetic features in the post-transplant setting. Conclusion: Further studies are warranted to clarify the particular role of sTIM-3 in the allo-HSCT setting.


Assuntos
Receptor Celular 2 do Vírus da Hepatite A/metabolismo , Leucemia Mieloide Aguda/terapia , Transplante/métodos , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
19.
Rev. bioét. (Impr.) ; 28(1): 83-88, jan.-mar. 2020.
Artigo em Inglês | LILACS | ID: biblio-1092425

RESUMO

Abstract We verified moral distress related to organ shortage for transplantation in nursing students. This quantitative pilot study analyzed data from 104 nursing undergraduate students. Data were collected through a survey composed of four questions and two sociodemographic items. The chi-squared test was used to examine categorical variables, whereas continuous variable data were analyzed using ANOVA and the Pearson Product Moment correlational test for determining the existence of moral distress regarding the availability of one heart for four individuals susceptible to heart transplantation. A high level of moral distress was identified with regard to the hypothetical decision-making process, which justifies the need for further studies on the subject. Given the hypothetical scenario, moral distress was observed among the students, reaching severe distress in some cases. Approval CEP-University of Alberta Pro00068610


Resumen Nuestro objetivo con esta investigación fue identificar la angustia moral en estudiantes de enfermería relacionada con la escasez de órganos para trasplante. Este es un estudio piloto cuantitativo que analizó datos de 104 estudiantes de grado de enfermería. Los datos fueron recolectados a través de una investigación compuesta por 4 preguntas y 2 ítems sociodemográficos. Posteriormente, se utilizó la prueba de Chi-cuadrado para examinar las variables categóricas, mientras que las variables continuas se analizaron utilizando las pruebas correlacionales Anova y Pearson Product Moment para determinar la existencia de angustia moral en relación con la disponibilidad de un corazón para cuatro personas que requieren el trasplante. Se identificó un alto nivel de angustia moral en relación con el hipotético proceso de toma de decisiones, lo que justifica la necesidad de realizar más estudios sobre el tema. Dada la situación hipotética, la angustia moral entre los estudiantes, incluida la angustia severa, es notable. Aprobado CEP-University of Alberta Pro00068610


Resumo Objetivou-se identificar sofrimento moral em estudantes de enfermagem relacionado à escassez de órgãos para transplante. Este estudo-piloto quantitativo analisou dados de 104 graduandos de enfermagem. Os dados foram coletados por meio de questionário composto de quatro questões e dois itens sociodemográficos. O teste qui--quadrado foi usado para examinar as variáveis categóricas, enquanto as variáveis contínuas foram analisadas utilizando os testes correlacionais Anova e Pearson Product Moment a fim de determinar a existência de sofrimento moral quanto à disponibilidade de um coração para quatro indivíduos que necessitam de transplante. Identificou-se alto nível de sofrimento moral relacionado ao processo de tomada de decisão hipotético, o que justifica a necessidade de novos estudos acerca do tema. Diante da situação hipotética, observou-se sofrimento moral entre estudantes, incluindo sofrimento severo. Aprovação CEP-University of Alberta Pro00068610


Assuntos
Humanos , Masculino , Feminino , Estresse Psicológico , Transplante , Obtenção de Tecidos e Órgãos , Tomada de Decisões , Ética , Enfermeiros
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