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1.
Bratisl Lek Listy ; 121(2): 154-158, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32115970

RESUMO

OBJECTIVE: This study aimed to evaluate the effect of Coenzyme Q10 (CoQ10) administration to patients in the early phase of sepsis to determine its effect on the markers of inflammation and the clinical outcomes of septic patients. BACKGROUND: Previous studies showed that CoQ10 levels were decreased in septic patients and worsening of mitochondrial dysfunction was observed. METHODS: In this randomized controlled trial septic patients (n=40) received 100 mg CoQ10 twice a day for seven days added to standard treatment of sepsis. As a primary endpoint levels of Interleukin 6 (IL-6), Tumor Necrosis Factor-α (TNF-α), Glutathione peroxidase and malondialdehyde (MDA) were assessed at baseline, third and 7th day after the intervention. Secondary endpoints included assessment of clinical scores and     in-hospital mortality. RESULTS: There was no difference in baseline inflammatory and oxidative injury markers between the groups. TNF-α and MDA levels decreased significantly in the CoQ10 group on the 7th day of the study (P:0.003 for both). There was a significant difference in the in-hospital mortality in the CoQ10 group compared to the control group (P:0.01). CONCLUSION: These findings suggest that CoQ10 has a positive effect on clinical parameters as well as mitochondrial dysfunction when administered in the early phase of sepsis (Tab. 2, Fig. 1, Ref. 38).


Assuntos
Inflamação , Sepse , Ubiquinona/análogos & derivados , Biomarcadores , Método Duplo-Cego , Humanos , Inflamação/tratamento farmacológico , Malondialdeído , Sepse/tratamento farmacológico , Sepse/imunologia , Ubiquinona/uso terapêutico
2.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 45(1): 24-34, 2020 Jan 28.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-32132294

RESUMO

OBJECTIVES: To examine the changes of coenzyme Q10 (CoQ10) and ß1,3-galactosyl transferase specific chaperone 1 (C1GALT1C1) in brain of rats with ischemic injury at different time points and to explore the protective mechanism of ultrashort wave (USW) on ischemic brain injury. METHODS: Fifty SD rats were randomly divided into 5 groups (n=10 per group): a sham group (control group) and 4 experimental group (ischemia for 2 h). The 4 experimental groups were set as a model 1 d group, a USW 1 d group, a model 3 d group and a USW 3 d group, respectively. Five rats were randomly selected for 2,3,5-triphenyltetrazoliumchloride (TTC) staining in each experimental group, and the remaining 5 rats were subjected to Western blotting and real-time PCR. The percentage of cerebral infarction volume and the relative expression level of CoQ10 and C1GALT1C1 in the brain were examined and compared. RESULTS: The infarct volume percentage after TTC staining was zero in the sham group. With the progress of disease and USW therapy, the infarct volume percentage was decreased in the experimental groups (all P<0.05); Western blotting and real-time PCR showed that the relative expression level of CoQ10 in the sham group was the highest, while in the experimental groups, the content of CoQ10 showed a upward trend with the extension of disease and USW therapy, with significant difference (all P<0.05). The relative expression level of C1GALT1C1 in the sham group was the lowest, but in the experimental groups, they showed a downward trend with the extension of disease and USW therapy, with significant difference (all P<0.05). CONCLUSIONS: Non-caloric USW therapy may upregulate the expression of CoQ10 to suppress the expression of C1GALT1C1 in rats, leading to alleviating cerebral ischemic reperfusion injury.


Assuntos
Isquemia Encefálica , Traumatismo por Reperfusão , Animais , Encéfalo , Chaperonas Moleculares , Ratos , Ratos Sprague-Dawley , Ubiquinona/análogos & derivados
4.
J Agric Food Chem ; 68(5): 1405-1418, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31940190

RESUMO

The aim of this study was to develop a novel system for the co-delivery of resveratrol and coenzyme Q10 (CoQ10). It was achieved with a combination of resveratrol-loaded composite nanoparticles and CoQ10-loaded Pickering emulsions. Different levels of resveratrol (0.05-0.30%, w/v) were entrapped into composite nanoparticles by the method of emulsification-evaporation. The size of composite nanoparticles was around 300-600 nm, and the maximum loading capacity of resveratrol was up to 13.88% (w/w). Hydrogen bonds, hydrophobic effects, and electrostatic attraction participated in the self-assembly of composite nanoparticles. The stability of CoQ10 Pickering emulsions was monitored under simulated environmental stresses (pH, ionic strength, UV radiation, and heat) and accelerated storage conditions. The physical stability of Pickering emulsions was dependent on the particle compositions, and the CoQ10 entrapped was also protected by the resveratrol-loaded nanoparticles. The morphology of Pickering emulsions was observed with the aid of optical microscopy, confocal laser scanning microscopy, and cryo-scanning electronic microscopy. The nutraceutical Pickering emulsions were designed for the co-delivery of resveratrol and CoQ10, which has the potential to be a novel vehicle for bioactive ingredients.


Assuntos
Sistemas de Liberação de Medicamentos/métodos , Nanopartículas/química , Resveratrol/química , Ubiquinona/análogos & derivados , Estabilidade de Medicamentos , Emulsões/química , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Concentração Osmolar , Tamanho da Partícula , Ubiquinona/química
5.
Int J Syst Evol Microbiol ; 70(2): 1042-1047, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31999241

RESUMO

Designated strain K5T was isolated from soil on Jeju Island. The bacterium was aerobic, Gram-stain-negative, oxidase-positive, catalase-low activity, motile, short-rod shaped, opaque and formed white colonies that were circular, raised and had entire margins. Strain K5T was able to grow at 15-40 °C, pH 4-9 and at 0-2 % (w/v) NaCl concentration. Phylogenetic analysis based on its 16S rRNA gene sequences indicated that strain K5T is closely related to Ferrovibrio soli A15T (98.9 % sequence similarity), Ferrovibrio denitrificans Sp-1T (98.7 %) and Ferrovibrio xuzhouensis LM-6T (97.4 %). The sole respiratory quinone was determined to be ubiquinone-10. The dominant fatty acids of strain K5T were summed feature 8 (C18 : 1 ω7c / C18 : 1ω6c, 29.8 %), C19 : 0 cyclo ω8c (20.2 %) and C16 : 0 (24.4 %). DNA G+C content was 63.6 % and DNA-DNA relatedness between strain K5T and other three members of the genus Ferrovibrio ranged from 24 to 28 %. The major polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine and aminolipids. Moreover, polyphasic characterization revealed that strain K5T represents a novel species in the genus Ferrovibrio, for which the name Ferrovibrio terrae sp. nov. is proposed. The type strain is K5T (=KCCM 43295T=LMG 30611T).


Assuntos
Filogenia , Rhodospirillaceae/classificação , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Ilhas , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Rhodospirillaceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
6.
Int J Syst Evol Microbiol ; 70(1): 675-679, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31671046

RESUMO

A taxonomic study was performed on strain GCJ02T, which was isolated from forest soil from Baishan City, PR China. The bacterium was Gram-stain-positive, catalase-positive and weakly oxidase-positive, rod-shaped and non-motile. Growth was observed at salinities of 0-6.0 % and at temperatures of 4-26 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that GCJ02T represented a member of the genus Cryobacterium, with the highest sequence similarity to Cryobacterium arcticum SK1T (99.5 %) and Cryobacterium zongtaii TMN-42T (99.5 %), followed by Cryobacterium psychrotolerans CGMCC 1.5382T (97.7 %), and other species of the genus Cryobacterium (96.4-96.9 %). The ANI and the DNA-DNA hybridization estimate values between GCJ02T and all type strains of species of the genus Cryobacterium were 72.5-84.5 % and 19.6-28.7 %, respectively. The principal fatty acids (>10 %) of GCJ02T were anteiso-C15 : 0(53.0 %) and anteiso-C17 : 0 (18.8 %). The G+C content of the chromosomal DNA was 68.4 mol%. The respiratory quinone was determined to be MK-10. Phosphatidylglycerol, diphosphatidylglycerol, glycolipid, and one unidentified phospholipid and three unidentified polar lipid were present. The combined genotypic and phenotypic data indicated that strain GCJ02T represents a novel species within the genus Cryobacterium, for which the name Cryobacterium soli sp. nov. is proposed, with the type strain GCJ02T (=MCCC 1K03549T=JCM 32391T).


Assuntos
Actinobacteria/classificação , Florestas , Filogenia , Microbiologia do Solo , Actinobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
7.
Int J Syst Evol Microbiol ; 70(1): 662-667, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31661050

RESUMO

A Gram-stain-negative, motile, alkali-tolerant, swollen-rod shaped, reddish brown coloured, phototrophic bacterium designated as strain JA980T, was isolated from freshwater sampled at Umiam lake, Shillong, India. Strain JA980T grew well up to pH 9.0. Respiratory quinones were ubiquinone 10 and rhodoquinone 10. The major fatty acid was C18: 1ω7c/C18:1ω6c with minor amounts of C18:0, C16:0, C18:0 3-OH and C16:0 3-OH. Strain JA980T contained bacteriochlorophyll-a and carotenoids of the spirilloxanthin series. The polar lipids of strain JA980T comprised phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, an unidentified phospholipid, unidentified amino lipids (AL1,3,4,5) and an unidentified lipid (L1). Strain JA980T had the highest (99.57 %) 16S rRNA gene sequence similarity to the type strains of Rhodomicrobium vannielii ATCC17100T and Rhodomicrobium udaipurense JA643T. The genome of strain JA980T was 3.88 Mbp with a DNA G+C content of 62.4 mol%. Based on the results of phylogenetic analyses, low in silico DNA-DNA hybridization values (33 %), low (87 %) average nucleotide identity results, chemotaxonomic characteristics and differential physiological properties, strain JA980T could not be classified into either of the two recognized species of the genus Rhodomicrobium, suggesting that it represents a novel species, for which the name Rhodomicrobium lacus sp. nov. is proposed. The type strain is JA980T (=KCTC 15697T= MCC 3714T= NBRC 113803T).


Assuntos
Lagos/microbiologia , Filogenia , Rhodomicrobium/classificação , Técnicas de Tipagem Bacteriana , Bacterioclorofila A/química , Composição de Bases , Carotenoides/química , DNA Bacteriano/genética , Ácidos Graxos/química , Índia , Hibridização de Ácido Nucleico , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Rhodomicrobium/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química , Microbiologia da Água
8.
Int J Syst Evol Microbiol ; 70(1): 668-674, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31665097

RESUMO

A novel Gram-reaction-negative, facultative-aerobic, motile, non-spore-forming, oval-shaped and denitrifying bacterium, designated BO-81T, was isolated from sludge sampled in the Republic of Korea. This bacterium was investigated via a polyphasic approach to reveal its taxonomic position. The results of phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain BO-81T belonged to the family Rhodobacteraceae and was related to the closest species Sinirhodobacter ferrireducens (98.8 % sequence similarity), 'Sinorhodobacter hungdaonensis' (98.4 %), Rhodobacter lacus (97.8 %), Sinorhodobacter populi (96.8 %) and Rhodobacter maris (96.2 %). The average nucleotide identity and DNA-DNA hybridization values between strain BO-81T and its closely related strains were 77.7-94.2 % and 20.1-55.9 %, respectively, indicating that BO-81T represents a novel species of the genus Sinirhodobacter. Growth occurred at 18-40 °C on Reasoner's 2A medium in the presence of 0-7 % NaCl (w/v) and at pH 6.0-9.0. Strain BO-81T was characterized chemotaxonomically as having ubiquinone 10 as its predominant respiratory quinone, summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) as its major fatty acid and phosphatidylglycerol and phosphatidylethanolamine as its predominant polar lipids. The G+C content of the genomic DNA was 68.3 mol%. The results of physiological and biochemical tests allowed phenotypic differentiation of strain BO-81T from other Sinirhodobacter species with validly published names. Therefore, the isolate represented a novel species, for which the name Sinirhodobacter hankyongi sp. nov. (type strain BO-81T=KACC 19677T=LMG 30808T) is proposed.


Assuntos
Filogenia , Rhodobacteraceae/classificação , Esgotos/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Desnitrificação , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
9.
Int J Syst Evol Microbiol ; 70(1): 274-281, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31702535

RESUMO

A taxonomic study was carried out on strain HN-E21T, which was isolated from sponge collected from Yangpu Bay, Hainan, PR China. Cells of strain HN-E21T were Gram-stain-negative, non-spore-forming, tumbling-motile, ovoid- to rod-shaped and pale-yellow-pigmented that could grow at 15-42 °C (optimum, 37 °C), at pH 6-11 (pH 7) and in 0-14 % (w/v) NaCl (2-3 %). This isolate was positive for oxidase and catalase, but negative for hydrolysis of aesculin and gelatin. The phylogenetic tree based on 16S rRNA gene sequences showed that strain HN-E21T formed a clade with Pelagivirga sediminicola BH-SD19T, Pontibaca methylaminivorans GRP21T, Roseovarius antarcticus M-S13-148T, Roseovarius aquimarinus CAU1059T and Roseovarius nanhaiticus NH52JT within the family Rhodobacteraceae. Strain HN-E21T shared the highest similarity to Pelagivirga sediminicola BH-SD19T (95.5 %), followed by Roseovarius lutimaris 112T (95.3 %), Pelagicola litorisediminis D1-W8T (95.2 %), Roseovarius gaetbuli YM-20T (95.0 %) and Roseovarius marisflavi H50T (95.0 %). The dominant fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16:0. The major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, three unidentified lipids and one unidentified phospholipid. The respiratory quinone was identified as Q-10. The G+C content of the genomic DNA was 66.4 mol%. Based on the phenotypic and phylogenetic data, strain HN-E21T represents a novel species of the genus Roseovarius, for which the name Roseovarius spongiae sp. nov. is proposed, with the type strain HN-E21T (=MCCC 1K03333T=LMG 30456T).


Assuntos
Filogenia , Poríferos/microbiologia , Rhodobacteraceae/classificação , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
10.
Int J Syst Evol Microbiol ; 70(1): 680-686, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31675287

RESUMO

A salt-tolerant, Gram-negative, rod-shaped and yellow-pigmented bacterium, designated strain AY-3RT, was isolated from rhizosphere soil of a desert xerophyte, Haloxylon ammodendron, sampled at Badain Jaran Desert, Alxa region, Inner Mongolia, PR China. Growth of this strain was observed at 20-42 °C (optimum, 28-30 °C), at pH 6.0-9.0 (optimum, pH 6.0-7.0) and at 0-8 % (w/v) NaCl (optimum, 3 %). Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain AY-3RT was a member of the genus Altererythrobacter, with the highest similarity to Altererythrobacter aerophilus Ery1T (97.6 %), followed by Altererythrobacter xinjiangensis S3-63T (96.9 %). The predominant fatty acids (>10.0 %) were C18 : 1ω7c, C17 : 1ω6c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and one unknown polar lipid. The predominant respiratory quinone was ubiquinone-10. The G+C content of the genomic DNA of strain AY-3RT was 66.3 mol%. On the basis of the data from this polyphasic taxonomic study, strain AY-3RT represents a novel species of the genus Altererythrobacter, named Altererythrobacter rhizovicinus sp. nov. (=MCCC 1K03572T=KCTC 72280T).


Assuntos
Alphaproteobacteria/classificação , Chenopodiaceae/microbiologia , Filogenia , Rizosfera , Microbiologia do Solo , Alphaproteobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
11.
Int J Syst Evol Microbiol ; 70(1): 687-692, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31778348

RESUMO

A Gram-stain negative, aerobic, motile and rod-shaped bacterium, designated strain 3.1105T, was isolated from a karst district soil sample collected from Tiandong cave, Guizhou province, south-west PR China. The isolate grew at 10-40 °C and pH 5.0-8.0 and tolerated up to 1 % NaCl (w/v) on R2A medium, with optimal growth at 25-30 °C, pH 7.0 and 0 % NaCl (w/v). Cells showed oxidase-positive and catalase-positive reactions. The respiratory quinone was Q-10. The predominant cellular fatty acids contained C18 : 1ω7c 11-methyl, summed feature 8 (C18 : 1ω7c or C18 : 1ω6c), C16 : 0 and C17 : 0. The major polar lipids were phosphatidylglycerol and monoglycosyldiglycerides. The genomic DNA G+C content was 56.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that 3.1105T should be affiliated to the genus Asticcacaulis and showed highest 16S rRNA gene sequence similarity values with Asticcacaulis excentricus CB 48T (96.0 %), Asticcacaulis endophyticus ZFGT-14T (95.3 %) and lower than 95.3 % similarity to other species of the genus Asticcacaulis. The polyphasic taxonomic characteristics indicated that strain 3.1105T represents a novel species of the genus Asticcacaulis, for which the name Asticcacaulis tiandongensis sp. nov., (type strain 3.1105T=KCTC 62978T=CCTCC AB 2018268T) is proposed.


Assuntos
Caulobacteraceae/classificação , Cavernas/microbiologia , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , Caulobacteraceae/isolamento & purificação , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
12.
Int J Syst Evol Microbiol ; 70(1): 550-554, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31651377

RESUMO

A novel Gram-staining negative, aerobic, motile by flagellum, rod-shaped bacterium, designated CFH 70021T was isolated from a hot spring soil sample collected from Tengchong, Yunnan province, PR China. Growth of CFH 70021T occurred at 15-50 °C (optimum 50 °C), pH 5.0-7.0 (optimum pH 7.0) and with 0-3.0 % (w/v) NaCl (optimum 0 %, w/v). The genome of CFH 70021T consisted of four complete circular chromosomes and five plasmids, the genomic DNA G+C content was 69.3 mol%. Comparison of the 16S rRNA gene sequences indicated that CFH 70021T represented a member of the genus Azospirillum and showed close relationship with the type strains of Azospirillum agricola CC-HIH038T (97.8 %), Azospirillum rugosum IMMIB AFH-6T (97.6 %), Azospirillum doebereinerae GSF71T (97.6 %), Azospirillum thiophilum DSM 21654T (97.4 %) and Azospirillum picis IMMIB TAR-3T (97.2 %). The polar lipids of CFH 70021T contained diphosphatidylglycerol, phosphatidylmehtylethanolamine, phosphatidylglycerol, phosphatidylcholine, two aminolipids and an unidentified phospholipid. The predominant cellular fatty acids (>10 %) included C19:0cyclo ω8c (11.4 %), C16 : 0 (27.6 %) and summed feature 8 (C18:1ω7c/C18:1ω6c, 40.9 %). The major isoprenoid quinone was Q-10. On the basis of the low ANIb result (<78 %) and different phenotypic and chemotaxonomic characters, we conclude that strain CFH 70021T represents a novel member of the genus Azospirillum, for which the name Azospirillum thermophilum sp. nov. is proposed. The type strain is CFH 70021T (=KCTC 62259T= CCTCC AB2018121T).


Assuntos
Azospirillum/classificação , Fontes Termais/microbiologia , Filogenia , Microbiologia do Solo , Azospirillum/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
13.
Int J Syst Evol Microbiol ; 70(1): 373-379, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31613738

RESUMO

A novel alphaproteobacterium, strain WS11T, was isolated from a deep-sea sediment sample collected from the New Britain Trench. The full-length 16S rRNA gene of strain WS11T had the highest sequence similarity of 97.6 % to Rhizobium subbaraonis JC85T, followed by Mycoplana ramosa DSM 7292T (96.9 %) and Rhizobium azooxidifex Po 20/26T (96.8 %). Phylogenetic analysis of concatenated 16S rRNA, atpD and recA gene sequences showed that strain WS11T was deeply separated from the species within the family Rhizobiaceae. Phylogenomic analysis based on the whole-genome protein sequences showed that strain WS11T formed an independent monophyletic branch in the family Rhizobiaceae, paralleled with the species in the families Brucellaceae and Phyllobacteriaceae within the order Rhizobiales. Cells were Gram-stain-negative, oxidase- and catalase-positive, and aerobic short rods (1.5-2.4×0.9-1.0 µm). Growth was observed at salinities ranging from 0 to 5% (optimum, 1 %), from pH 6.5 to 9 (optimum, pH 7) and at temperatures between 20 and 30 °C (optimum, 28 °C). Strain WS11T was piezotolerant, growing optimally at 0.1 MPa (range 0.1-70 MPa). The main fatty acid was summed feature 8 (C18 : 1 ω7c/C18  : 1 ω 6c). The sole respiratory quinone was ubiquinone-10 (Q-10). The predominant polar lipids were phosphatidylcholine, two unidentified aminophospholipids and an unidentified phospholipid. The genome size was about 4.36 Mbp and the G+C content was 62.3 mol%. The combined genotypic and phenotypic data show that strain WS11T represents a novel species of a novel genus in the family Rhizobiaceae, for which the name Georhizobium profundi gen. nov., sp. nov. is proposed (type strain WS11T=MCCC 1K03498T=KCTC 62439T).


Assuntos
Alphaproteobacteria/classificação , Sedimentos Geológicos/microbiologia , Filogenia , Água do Mar/microbiologia , Alphaproteobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Oceano Pacífico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
14.
Int J Syst Evol Microbiol ; 70(1): 582-588, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31613741

RESUMO

A nonphotosynthetic, Gram-stain-negative, rod-shaped and motile strain, designated Pet-1T, was isolated from oil-contaminated soil collected from Daqing oil field in China. Optimal growth occurred at 37 °C, pH 5.5 and in 1 % (w/v) NaCl. Q-10 was the sole respiratory quinone. The most abundant fatty acid was C18 : 1É·7c/C18 : 1É·6c (67.4 %). The major polar lipids were phosphatidylglycerol, aminolipid, phosphatidylethanolaine, phosphatidycholine, two unidentified lipids and two unidentified phospholipids. The genomic DNA G+C content was 69.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that Pet-1T shared the highest similarity (95.1 %) to Rhodobacter vinaykumarii DSM 18714T, followed by Sinorhodobacter populi sk2b1T (95.0 %) and Haematobacter massiliensis CCUG 47968T (95.0 %). In the phylogenetic tree, strain Pet-1T formed a separate branch from the closely related genera Rhodobacter, Pararhodobacter, Defluviimonas and Rhodovulum within the family Rhodobacteraceae. Based on the data from the current polyphasic study, it is proposed that the isolate is a novel species of a novel genus within the family Rhodobacteraceae, with the name Solirhodobacter olei gen. nov., sp. nov. The type strain of the type species is Pet-1T (=KCTC 72074T =CCTCC AB 2018368T).


Assuntos
Poluição por Petróleo , Filogenia , Rhodobacteraceae/classificação , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
15.
Int J Syst Evol Microbiol ; 70(1): 251-258, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31622229

RESUMO

Thermotolerant bacterial nanocellulose-producing strains, designated MSKU 9T and MSKU 15, were isolated from persimmon and sapodilla fruits, respectively. These strains were aerobic, Gram-stain-negative, had rod-shaped cells, were non-motile and formed white-cream colonies. Phylogeny based on the 16S rRNA gene sequences revealed that MSKU 9T and MSKU 15 represented members of the genus Komagataeibacter and formed a monophyletic branch with K. swingsii JCM 17123T and K. europaeus DSM 6160T. The genomic analysis revealed that overall genomic relatedness index values of MSKU 9T with K. swingsii JCM 17123T and K. europaeus DSM 6160T were ~90 % average nucleotide identity (ANI) and ≤58.2 % digital DNA-DNA hybridization (dDDH), respectively. MSKU 9T and MSKU 15 can be differentiated from the closely related K. swingsii JCM 17123T by their growth on 30 % d-glucose and ability to utilize and to form acid from raffinose and sucrose as carbon sources, and from K. europaeus DSM 6160T by their ability to grow without acetic acid. The genomic DNA G+C contents of MSKU 9T and MSKU 15 were 60.4 and 60.2 mol%, respectively. The major fatty acids of MSKU 9T and MSKU 15 were summed feature 8 (C18 : 1 ω7c and/or C18  : 1ω6c). The respiratory quinone was determined to be Q10. On the basis of the results of the polyphasic taxonomic analysis, MSKU 9T (=TBRC 9844T=NBRC 113802T) represents a novel species of the genus Komagataeibacter, for which the name Komagataeibacter diospyri sp. nov. is proposed.


Assuntos
Acetobacteraceae/classificação , Diospyros/microbiologia , Manilkara/microbiologia , Filogenia , Acetobacteraceae/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Frutas/microbiologia , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Tailândia , Ubiquinona/análogos & derivados , Ubiquinona/química
16.
Int J Syst Evol Microbiol ; 70(1): 334-339, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31622234

RESUMO

A novel Gram-stain-negative, rod-shaped, non-spore-forming, non-flagellated, strictly aerobic strain, designated A6024T, was isolated from coastal seawater near Rizhao, PR China (119.61° E 35.47° N). The organism grew optimally at 28 °C, in pH 6.0-7.0 and in the presence of 3.0 % (w/v) NaCl. The strain required seawater or artificial seawater for growth and NaCl alone did not support growth. Strain A6024T contained ubiquinone 10 as the sole respiratory quinone and C18 : 1 ω7c (75.2 %) as the most abundant fatty acid. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content of strain A6024T was 59.9 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aliiroseovarius halocynthiae MA1-10T, Aliiroseovarius pelagivivens GYSW-22T and Aliiroseovarius crassostreae CV919-312T with 98.3, 97.6 and 97.4 % sequence similarities, respectively. The calculated average nucleotide identity values and DNA-DNA hybridization values between strain A6024T and the phylogenetically related Aliiroseovarius species were in the range 76.0-85.6 % and 19.6-29.4 %, respectively. On the basis of the results of polyphasic analyses, strain A6024T represents a novel species of the genus Aliiroseovarius, for which the name Aliiroseovarius marinus sp. nov. is proposed. The type strain is A6024T (=KCTC 72114T=MCCC 1K03595T).


Assuntos
Filogenia , Rhodobacteraceae/classificação , Água do Mar/microbiologia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
17.
Int J Syst Evol Microbiol ; 70(1): 522-529, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31596192

RESUMO

A yellow-coloured, Gram-negative, motile, strictly aerobic bacterial strain, designated strain DAC4T, was isolated from a soil sample collected at Ahnmok Beach (Busan, Republic of Korea). The cells of strain DAC4T were rod-shaped and the colonies that formed were round and convex. The results of phylogenetic analysis based on the 16S rRNA gene sequence of strain DAC4T revealed that the bacterium belongs to the genus Sphingomonas, family Sphingomonadaceae, and that it was most closely related to Sphingomonas jaspsi DSM 18422T (98.01 %), Sphingomonas rhizophila KACC 19189T (97.76 %), Sphingomonas mesophila KCTC 62179T(97.30 %), Sphingomonas sedimincola KCTC 12629T (97.16 %) and Sphingomonas oryziterrae KCTC 22476T (97.05 %). The major respiratory quinone was Q-10, and the major cellular fatty acids were summed feature 8 (C18  :  1ω7c) and summed feature 3 (C16  :  1ω7c/C16  :  1ω6c). The whole genome DNA G+C content of strain DAC4T was 62.16 mol%. Phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipids, phosphatidylglycerol, phosphatidylcholine, four undefined glycolipids and an undefined lipid were detected in strain DAC4T, and the strain had sym-homospermidine as a major polyamine. The in silico DNA-DNA hybridization and average nucleotide identity values between strain DAC4T and the closely related taxa S. jaspsi and S. mesophila were 75.5/23.5 % and 73.5 /18.5%, respectively. The fluorimetric DNA-DNA hybridization results showed that strain DAC4T and S. rhizophila, S. sediminicola and S. oryziterrae have 37.1, 35.2 and 32.2 % DNA similarity, respectively. Based on phylogenetic, phenotypic and chemotaxonomic distinctiveness, strain DAC4T (=KCTC 62107T=JCM 32377T) is classified as a novel species of the genus Sphingomonas, for which the name Sphingomonas edaphi sp. nov. is proposed.


Assuntos
Praias , Filogenia , Microbiologia do Solo , Sphingomonas/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Espermidina/análogos & derivados , Espermidina/química , Sphingomonas/isolamento & purificação , Ubiquinona/análogos & derivados , Ubiquinona/química
18.
Int J Syst Evol Microbiol ; 70(1): 204-210, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31596194

RESUMO

A Gram-stain-negative, rod-shaped, motile, facultatively aerobic and ivory-pigmented bacterium (designated strain LA-55T) was isolated from a river in the Republic of Korea. On the basis of 16S rRNA gene sequencing, strain LA-55T clustered with species of the genus Brevundimonas and was closely related to B revundimonas kwangchunensis KSL-102T (97.3 %), B revundimonas aurantiaca DSM 4731T (97.1 %), B revundimonas albigilva NHI-13T (97.0 %), B revundimonas balnearis FDRGB2bT (97.0 %) and Brevundimonas aveniformis DSM 17977T (97.0 %). The average nucleotide identity value between strain LA-55T and its closest-related strain was 74.1 %, indicating that strain LA-55T represents a novel species of the genus Brevundimonas. Growth occurred at 15-40 °C on Reasoner's 2A medium in the presence of 0-2 % NaCl (w/v) and at pH 6.0-8.0. The genomic DNA G+C content was 70.5 mol% and ubiquinone 10 (Q-10) was the major respiratory quinone. The major cellular fatty acids (>5 %) were C1 8 :1 ω6c and/or C1 8 :1 ω7c (summed feature 8), C16 : 0, C1 6 :1 ω6c and/or C1 6 :1 ω7c (summed feature 3) and C18 : 1 ω7c 11-methyl. The polar lipids consisted of phosphatidylglycerol, 1,2-di-O-acyl-3-O-[d-glucopyranosyl-(1→4)-α-d-glucopyranuronosyl]glycerol, 1,2-di-O-acyl-3-O-α-d-glucopyranuronosyl glycerol, unidentified aminolipid, unidentified phosphoglycolipid and unidentified lipids. Physiological and biochemical characteristics indicated that strain LA-55T represents a novel species of the genus Brevundimonas, for which the name Brevundimonas fluminis sp. nov. is proposed. The type strain is LA-55T (=KACC 19639T=LMG 30850T).


Assuntos
Caulobacteraceae/classificação , Filogenia , Rios/microbiologia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , Caulobacteraceae/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
19.
Int J Syst Evol Microbiol ; 70(1): 340-345, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31596199

RESUMO

A Gram-stain-negative bacterium, designated IO390501T, was isolated from a sea water sample from the Indian Ocean and taxonomically characterized using a polyphasic approach. The strain is phylogenetically close to 'Devosia lucknowensis' L15 and Devosia chinhatensis IPL18T, with 16S rRNA gene sequence similarity of 97.7 and 97.4 %, respectively. The genome of IO390501T has a DNA G+C content of 61.9 mol% for the 3.9 Mb chromosome. Genome-based phylogenetic trees indicated that IO390501T clusters as an independent lineage with 'D. lucknowensis' L15. Genomic relatedness of in silico DNA-DNA hybridization between IO390501T and phylogenetic neighbours ranged from 18.8 to 21.5 %, below the cutoff of 70 %, and corresponding average nucleotide identity values were between 71.4 and 79.0 %, lower than the 95.0 % threshold. The predominant cellular fatty acids of IO390501T are summed feature 8 (C18 : 1ω7c/C18  : 1ω6c) and C16  :  0. IO390501T contains ubiquinone-10 as the sole respiratory quinone, and phosphatidylglycerol and glycolipids as the major polar lipids. On the basis of the results of phenotypic, chemotaxonomic and genetic analyses, strain IO390501T represents a novel species of the genus Devosia for which the name Devosia indica sp. nov. is proposed. The type strain is IO390501T.


Assuntos
Hyphomicrobiaceae/classificação , Filogenia , Água do Mar/microbiologia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Hyphomicrobiaceae/isolamento & purificação , Oceano Índico , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
20.
Int J Syst Evol Microbiol ; 70(1): 309-316, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31596696

RESUMO

Strain TLA-22T, isolated from a cold spring in Taiwan, was characterized using a polyphasic taxonomy approach. Cells were Gram-stain-negative, aerobic, poly-ß-hydroxybutyrate-accumulating, motile by means of a single polar flagellum, rod-shaped and formed bright yellow colonies. Optimal growth occurred at 20-25 °C, pH 6-6.5, and in the presence of 0.5 % NaCl. The major fatty acids of TLA-22T were C18 : 1 ω7 c and C17 : 1ω6c. The predominant hydroxy fatty acids were C15 : 0 2-OH and C14 : 0 2-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sphingoglycolipid, an unidentified aminophospholipid, an unidentified phospholipid and three unidentified lipids. TLA-22T contained spermidine as the major polyamine and putrescine as the minor component. The only isoprenoid quinone was Q-10. The genomic DNA G+C content of TLA-22T was 63.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that TLA-22T was a mem,ber of a phylogenetic lineage including members of the genus Sphingobium. TLA-22T was most closely related to Sphingobium aromaticiconvertens RW16T, with a 97.4 % 16S rRNA gene sequence similarity. TLA-22T showed 74.8-75.7 % average nucleotide identity and 20.1-22.0 % digital DNA-DNA hybridization identity with the strains of other species of the genus Sphingobium. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TLA-22T should be classified as representing a novel species of the genus Sphingobium, for which the name Sphingobium algorifonticola sp. nov. is proposed. The type strain is TLA-22T (=BCRC 81097T =LMG 30309T=KCTC 62189T).


Assuntos
Nascentes Naturais/microbiologia , Filogenia , Sphingomonadaceae/classificação , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hidroxibutiratos , Hibridização de Ácido Nucleico , Fosfolipídeos/química , Pigmentação , Poliésteres , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Espermidina/química , Sphingomonadaceae/isolamento & purificação , Taiwan , Ubiquinona/análogos & derivados , Ubiquinona/análise
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