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1.
Arch Virol ; 165(4): 989-991, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32170393

RESUMO

This work identified a novel rhabdo-like virus in a Chinese black cutworm (Agrotis ipsilon), which we tentatively named "Agrotis ipsilon virus" (AIpsV). The complete genome of AIpsV is 15,454 nucleotides in length and contains seven open reading frames, collectively encoding more than 160 amino acids. The AIpsV genome is predicted to encode three structural proteins, nucleoprotein (N), glycoprotein (G), and large polymerase protein (L), and four unknown proteins. Phylogenetic analysis revealed that the AIpsV clusters with Wuhan ant virus and Hubei rhabdo-like virus 1 within the rhabdo-like virus clade. The level of expression of AIpsV genes was found to be higher in the pupal and adult stages than in the egg and larval stages.


Assuntos
Genoma Viral , Vírus de Insetos/genética , Mariposas/virologia , Animais , Sequência de Bases , Vírus de Insetos/classificação , Vírus de Insetos/isolamento & purificação , Larva/crescimento & desenvolvimento , Larva/virologia , Mariposas/crescimento & desenvolvimento , Fases de Leitura Aberta , Filogenia , Proteínas Virais/genética , Sequenciamento Completo do Genoma
2.
Arch Virol ; 165(3): 771-774, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31960157

RESUMO

Long-distance migratory insects carry microorganisms that can potentially play a crucial role in the life cycles of their hosts. Here, we used Illumina and Sanger sequencing to determine the complete genome sequence of a novel circular Rep-encoding single-stranded (ss) DNA virus from an important migratory pest, Agrotis ipsilon (Hufnagel). The full genome of this new virus is about 2, 242 nt in length and shares 55-75% genome-wide pairwise sequence identity with members of the family Genomoviridae but 91% nucleotide sequence identity with finch-associated genomovirus 3 isolate S30P_D, which is tentatively abbreviated "FaGmV-3". Viral infection rates in A. ipsilon from Yantai, Langfang and Xinxiang were 4.5% (n = 88), 11.8% (n = 85) and 0% (n = 35), respectively. Phylogenetic analysis based on the deduced amino acid sequence of Rep indicated that the Agrotis ipsilon-associated virus is closely related to members of the genus Gemykibivirus, and we propose it to be a new member of this genus. Hence, it is tentatively named "Agrotis ipsilon-associated genomovirus" (AiGmV).


Assuntos
Vírus de DNA , Vírus de Insetos/classificação , Vírus de Insetos/genética , Mariposas/virologia , Sequência de Aminoácidos , Animais , Sequência de Bases , China , Vírus de DNA/classificação , Vírus de DNA/genética , Vírus de DNA/isolamento & purificação , DNA de Cadeia Simples/genética , Vírus de Insetos/isolamento & purificação , Análise de Sequência de DNA
3.
Virology ; 534: 72-79, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31207441

RESUMO

An analysis of transcriptomes from the antennae of the three South American stink bugs (Euschistus heros, Chinavia ubica, and Dichelops melacanthus) revealed the presence of picorna-like virus genome-length RNAs with high sequence identity to the genome of Halyomorpha halys virus (HhV), originally discovered in the transcriptome of the brown marmorated stink bug, Halyomorpha halys. Features of the genome, phylogenetic relationships to other viruses, and the appearances of virus-like particles isolated from host stink bugs all confirm that these viruses are iflaviruses and isolates of an undescribed species. Iflavirus RNAs were present at high levels (40%-90% of transcriptome reads) in the stink bug antennal transcriptomes. In whole-insect transcriptomes of H. halys, HhV reads were >500-fold more abundant in adults than in nymphs. We identified from field population a subject of species E. heros infected by this iflavirus. The results of the analysis suggest that these iflaviruses are able to produce large quantities of their RNAs without causing any obvious pathology to their hosts.


Assuntos
Heterópteros/virologia , Vírus de Insetos/isolamento & purificação , Animais , Genoma Viral , Heterópteros/classificação , Heterópteros/genética , Vírus de Insetos/classificação , Vírus de Insetos/genética , Filogenia , RNA Viral/genética
4.
Arch Virol ; 164(3): 907-911, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30656464

RESUMO

A novel negevirus, tentatively named Manglie virus (MaV), was isolated from Culex tritaeniorhynchus from the village of Manglie, Yunnan, China, in August 2011. It was identified by high-throughput sequencing of cell culture supernatants, and the complete genome was sequenced using an Illumina MiSeq sequencer. The complete MaV genome comprised 9,218 nt encoding three hypothetical proteins and had a poly(A) tail. BLASTn analysis showed that the genome had the greatest similarity to Ngewotan virus strain Nepal22, with query coverage of 100% and 79% identity. Genomic and phylogenetic analyses demonstrated that MaV should be considered a novel negevirus.


Assuntos
Culex/virologia , Genoma Viral , Vírus de Insetos/genética , Vírus de Insetos/isolamento & purificação , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação , Animais , Sequência de Bases , China , Vírus de Insetos/classificação , Filogenia , Vírus de RNA/classificação , Proteínas Virais/genética , Sequenciamento Completo do Genoma
5.
Arch Virol ; 164(2): 643-647, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30415391

RESUMO

Three novel RNA viruses, named Formica fusca virus 1 (GenBank accession no. MH477287), Lasius neglectus virus 2 (MH477288) and Myrmica scabrinodis virus 2 (MH477289), were discovered in ants collected in Cambridge, UK. The proposed virus names were given based on the hosts in which they were identified. The genome sequences were obtained using de novo transcriptome assembly of high-throughput RNA sequencing reads and confirmed by Sanger sequencing. Phylogenetic analysis showed that Formica fusca virus 1 grouped within the family Nyamiviridae, Lasius neglectus virus 2 grouped within the family Rhabdoviridae and Myrmica scabrinodis virus 2 belongs to the family Dicistroviridae. All three viruses are highly divergent from previously sequenced viruses.


Assuntos
Formigas/virologia , Vírus de Insetos/isolamento & purificação , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação , Animais , Genoma Viral , Sequenciamento de Nucleotídeos em Larga Escala , Vírus de Insetos/classificação , Vírus de Insetos/genética , Fases de Leitura Aberta , Filogenia , Vírus de RNA/classificação , Transcriptoma , Proteínas Virais/genética
6.
Transbound Emerg Dis ; 66(1): 43-46, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30375175

RESUMO

We report the detection of Moku virus in honey bees (Apis mellifera) collected in 2017 from hives with a history of attacks by invasive Asian hornets (Vespa velutina nigrithorax) in Belgium. End 2016, Moku virus was reported in Asian hornets from the same area. In addition, the Moku virus was already present in historical samples of bees collected in 2013, that is, 2 years after the official first detection of Asian hornets in the same area of Belgium. This study suggests a spread of Moku virus to honey bees with possible consequences.


Assuntos
Abelhas/virologia , Vírus de Insetos/isolamento & purificação , Infecções por Vírus de RNA/veterinária , Animais , Bélgica/epidemiologia , Vírus de Insetos/genética , Infecções por Vírus de RNA/epidemiologia , Infecções por Vírus de RNA/virologia , Vírus de RNA/genética , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Vespas/virologia
7.
J Invertebr Pathol ; 160: 1-7, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30448511

RESUMO

We characterize a novel picorna-like virus, named Helicoverpa armigera Nora virus (HaNV), with a genome length of 11,200 nts, the sequence of which was isolated from the lepidopteran host cotton bollworm Helicoverpa armigera, using RNA-Seq. Phylogenetic analysis, using the putative amino acid sequence of the conserved RNA-dependent RNA polymerase (RdRp) domain, indicated that HaNV clustered with Spodoptera exigua Nora virus, Drosophila Nora virus and Nasonia vitripennis virus-3 with a high bootstrap value (100%), which might indicate a new viral family within the order Picornavirales. HaNV was efficiently horizontally transmitted between hosts via contaminated food, and transmission was found to be dose-dependent (up to 100% efficiency with 109 viral copy number/µl). HaNV was also found to be transmitted vertically from parent to offspring, mainly through transovum transmission (virus contamination on the surface of the eggs), but having a lower transmission efficiency (around 43%). Infection distribution within the host was also investigated, with HaNV mainly found in only the gut of both adult moths and larvae (>90%). Moreover, our results showed that HaNV appears not to be an overtly pathogenic virus to its host.


Assuntos
Vírus de Insetos/isolamento & purificação , Mariposas/virologia , Picornaviridae/classificação , Infecções por Vírus de RNA/transmissão , Animais , Bioensaio , Vírus de Insetos/genética , Vírus de Insetos/patogenicidade , Larva/virologia , Filogenia , Picornaviridae/isolamento & purificação , Infecções por Vírus de RNA/virologia , RNA Viral/genética , RNA-Seq
8.
Virus Res ; 262: 37-47, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-29169832

RESUMO

Insects are the host and vector of diverse viruses including those that infect vertebrates, plants, and fungi. Recent wide-scale transcriptomic analyses have uncovered the existence of a number of novel insect viruses belonging to an alphavirus-like superfamily (virgavirus/negevirus-related lineage). In this study, through an in silico search using publicly available insect transcriptomic data, we found numerous virus-like sequences related to insect virga/nege-like viruses. Phylogenetic analysis showed that these novel viruses and related virus-like sequences fill the major phylogenetic gaps between insect and plant virga/negevirus lineages. Interestingly, one of the phylogenetic clades represents a unique insect-infecting virus group. Its members encode putative coat proteins which contained a conserved domain similar to that usually found in the coat protein of plant viruses in the family Virgaviridae. Furthermore, we discovered endogenous viral elements (EVEs) related to virga/nege-like viruses in the insect genomes, which enhances our understanding on their evolution. Database searches using the sequence of one member from this group revealed the presence of EVEs in a wide range of insect species, suggesting that there has been prevalent infection by this virus group since ancient times. Besides, we present detailed EVE integration profiles of this virus group in some species of the Bombus genus of bee families. A large variation in EVE patterns among Bombus species suggested that while some integration events occurred after the species divergence, others occurred before it. Our analyses support the view that insect and plant virga/nege-related viruses might share common virus origin(s).


Assuntos
Alphavirus/genética , Abelhas/virologia , Genoma de Inseto , Vírus de Insetos/genética , Integração Viral , Animais , Abelhas/genética , Evolução Molecular , Variação Genética , Genoma Viral , Vírus de Insetos/isolamento & purificação , Filogenia , Vírus de Plantas/genética , Vírus de Plantas/isolamento & purificação , Transcriptoma/genética
9.
Sci Rep ; 8(1): 13936, 2018 10 04.
Artigo em Inglês | MEDLINE | ID: mdl-30287824

RESUMO

Waves of highly infectious viruses sweeping through global honey bee populations have contributed to recent declines in honey bee health. Bees have been observed foraging on mushroom mycelium, suggesting that they may be deriving medicinal or nutritional value from fungi. Fungi are known to produce a wide array of chemicals with antimicrobial activity, including compounds active against bacteria, other fungi, or viruses. We tested extracts from the mycelium of multiple polypore fungal species known to have antiviral properties. Extracts from amadou (Fomes) and reishi (Ganoderma) fungi reduced the levels of honey bee deformed wing virus (DWV) and Lake Sinai virus (LSV) in a dose-dependent manner. In field trials, colonies fed Ganoderma resinaceum extract exhibited a 79-fold reduction in DWV and a 45,000-fold reduction in LSV compared to control colonies. These findings indicate honey bees may gain health benefits from fungi and their antimicrobial compounds.


Assuntos
Abelhas/efeitos dos fármacos , Colapso da Colônia/prevenção & controle , Coriolaceae/química , Ganoderma/química , Vírus de Insetos/isolamento & purificação , Micélio/química , Extratos Vegetais/farmacologia , Vírus de RNA/isolamento & purificação , Varroidae/virologia , Administração Oral , Animais , Abelhas/parasitologia , Abelhas/virologia , Coriolaceae/crescimento & desenvolvimento , Relação Dose-Resposta a Droga , Feminino , Ganoderma/crescimento & desenvolvimento , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico
10.
J Invertebr Pathol ; 154: 74-78, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29625149

RESUMO

The invasive insect pest Drosophila suzukii infests ripening fruits and causes extensive damage to crops in the northern hemisphere. Novel, environmentally friendly strategies to control the spread of this species are urgently needed, and one promising approach is the deployment of entomopathogenic viruses. Here we report the identification and characterization of two natural viruses associated with D. suzukii: Drosophila A virus (DAV) and La Jolla virus (LJV). Our work provides new tools for the development of biological control agents that protect crops against D. suzukii without a harmful impact on biodiversity.


Assuntos
Drosophila/virologia , Vírus de Insetos/isolamento & purificação , Animais , Feminino , Vírus de Insetos/classificação , Vírus de Insetos/genética , Virulência
11.
Biol Lett ; 14(2)2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29491032

RESUMO

Global declines of insect pollinators jeopardize the delivery of pollination services in both agricultural and natural ecosystems. The importance of infectious diseases has been documented in honeybees, but there is little information on the extent to which these diseases are shared with other pollinator orders. Here, we establish for the first time the presence of three important bee viruses in hoverfly pollinators (Diptera: Syrphidae): black queen cell virus (BQCV), sacbrood virus (SBV) and deformed wing virus strain B (DWV-B). These viruses were detected in two Eristalis species, which are behavioural and morphological bee mimics and share a foraging niche with honeybees. Nucleotide sequences of viruses isolated from the Eristalis species and Apis mellifera were up to 99 and 100% identical for the two viruses, suggesting that these pathogens are being shared freely between bees and hoverflies. Interestingly, while replicative intermediates (negative strand virus) were not detected in the hoverflies, viral titres of SBV were similar to those found in A. mellifera These results suggest that syrphid pollinators may play an important but previously unexplored role in pollinator disease dynamics.


Assuntos
Dípteros/virologia , Vírus de Insetos/fisiologia , Animais , Abelhas/virologia , Dicistroviridae/genética , Dicistroviridae/fisiologia , Vírus de Insetos/isolamento & purificação , Polinização , Vírus de RNA/genética , Vírus de RNA/fisiologia , Homologia de Sequência do Ácido Nucleico
12.
J Gen Virol ; 99(5): 710-716, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29580322

RESUMO

There is little information about commensal viruses in the white-backed planthopper, Sogatella furcifera, although it is an important agricultural insect. Here, two novel double-stranded RNA viruses related to the viruses in the family Totiviridae were identified using next-generation sequencing and tentatively named Sogatella furcifera totivirus 1 and 2 (SfTV1 and SfTV2). Their complete genomes consist of 6310 and 6303 nt, respectively, showing typical genomic features with viruses in the family Totiviridae. Identity, phylogenetic and conserved sequence analyses showed that SfTV1, SfTV2 and three other insect viruses may form a proposed novel genus of the family Totiviridae. Vertical transmission of the two viruses was highly efficient, and they were detected in all insect tissues and developmental stages, with the highest titres in the adult and in the haemolymph and reproductive organs. To our knowledge, this is the first report of viruses in the family Totiviridae found in a hemipteran insect.


Assuntos
Hemípteros/virologia , Vírus de Insetos/classificação , Totivirus/classificação , Animais , Genoma Viral , Hemolinfa/virologia , Sequenciamento de Nucleotídeos em Larga Escala , Vírus de Insetos/genética , Vírus de Insetos/isolamento & purificação , Filogenia , Simbiose , Totivirus/genética , Totivirus/isolamento & purificação , Viroses/transmissão
13.
Acta Vet Hung ; 66(1): 151-161, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29580079

RESUMO

The predominance of dietary viruses in bat guano samples had been described recently, suggesting a new opportunity to survey the prevalence and to detect new viruses of arthropods or even plant-infecting viruses circulating locally in the ecosystem. Here we describe the diversity of viruses belonging to the order Picornavirales in Hungarian insectivorous bat guano samples. The metagenomic analysis conducted on our samples has revealed the significant predominance of aphid lethal paralysis virus (ALPV) and Big Sioux River virus (BSRV) in Hungary for the first time. Phylogenetic analysis was used to clarify the relationship to previously identified ALPV strains infecting honey bees, showing that our strain possesses a close genetic relationship with the strains that have already been described as pathogenic to honey bees. Furthermore, studies have previously confirmed the ability of these viruses to replicate in adult honey bees; however, no signs related to these viruses have been revealed yet. With the identification of two recently described possibly honey bee infecting viruses for the first time in Hungary, our results might have importance for the health conditions of Hungarian honey bee colonies in the future.


Assuntos
Abelhas/virologia , Quirópteros , Fezes/virologia , Genoma Viral , Vírus de Insetos/isolamento & purificação , Metagenômica , Animais , Hungria , Vírus de Insetos/genética , Filogenia
14.
J Insect Sci ; 17(3)2017 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-28973572

RESUMO

Wolbachia are widely distributed bacterial endosymbionts of arthropods and filarial nematodes. These bacteria can affect host fitness in a variety of ways, such as protecting hosts against viruses and other pathogens. Here, we investigate the possible role of Wolbachia in the prevalence of the deformed wing virus (DWV), a highly virulent pathogen of honey bees (Apis mellifera) that is transmitted by parasitic Varroa mites (Varroa destructor). About 180 Varroa mites from 18 beehives were tested for infection with Wolbachia and DWV. We first screened for Wolbachia using two standard primers (wsp and 16S rDNA), and found 26% of the mites to be positive for Wolbachia using the wsp primer and 64% of the mites to be positive using the 16S rDNA primer. Using these intermediate Wolbachia frequencies, we then tested for statistical correlations with virus infection frequencies. The analysis revealed a significant positive correlation between DWV and Wolbachia using the wsp primer, but no significant association between DWV and Wolbachia using the 16S rDNA primer. In conclusion, there is no evidence for an anti-pathogenic effect of Wolbachia in V. destructor, but weak evidence for a pro-pathogenic effect. These results encourage further examination of Wolbachia-virus interactions in Varroa mites since an increased vector competence of the mites may significantly impact disease outbreaks in honey bees.


Assuntos
Vírus de Insetos/isolamento & purificação , Varroidae/virologia , Wolbachia/isolamento & purificação , Animais
15.
J Gen Virol ; 98(11): 2876-2881, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29048274

RESUMO

During an entomological surveillance for arthropod-borne viruses in the Philippines, we isolated a previously unrecognized virus from female Armigeres spp. mosquitoes. Whole-genome sequencing, genetic characterization and phylogenetic analysis revealed that the isolated virus, designated Armigeres iflavirus (ArIFV), is a novel member of the iflaviruses (genus Iflavirus, family Iflaviridae) and phylogenetically related to Moku virus, Hubei odonate virus 4, slow bee paralysis virus and Graminella nigrifrons virus 1. To our knowledge, this is the first successful isolation of iflavirus from a dipteran insect. Spherical ArIFV particles of approximately 30 nm in diameter contained at least three major structural proteins. ArIFV multiplied to high titres (~109 p.f.u. ml-1) and formed clear plaques in a mosquito cell line, C6/36. Our findings provide new insights into the infection mechanism, genetic diversity and evolution of the Iflaviridae family.


Assuntos
Culicidae/virologia , Vírus de Insetos/classificação , Vírus de Insetos/isolamento & purificação , Vírus de RNA/classificação , Vírus de RNA/isolamento & purificação , Animais , Linhagem Celular , Filipinas , Ensaio de Placa Viral , Proteínas Estruturais Virais/análise , Vírion/química , Vírion/ultraestrutura
16.
J Gen Virol ; 98(11): 2864-2875, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29058655

RESUMO

By providing pollination services, bees are among the most important insects, both in ecological and economical terms. Combined next-generation and classical sequencing approaches were applied to discover and study new insect viruses potentially harmful to bees. A bioinformatics virus discovery pipeline was used on individual Illumina transcriptomes of 13 wild bees from three species from the genus Halictus and 30 ants from six species of the genera Messor and Aphaenogaster. This allowed the discovery and description of three sequences of a new virus termed Halictus scabiosae Adlikon virus (HsAV). Phylogenetic analyses of ORF1, RNA-dependent RNA-polymerase (RdRp) and capsid genes showed that HsAV is closely related to (+)ssRNA viruses of the unassigned Sinaivirus genus but distant enough to belong to a different new genus we called Halictivirus. In addition, our study of ant transcriptomes revealed the first four sinaivirus sequences from ants (Messor barbarus, M. capitatus and M. concolor). Maximum likelihood phylogenetic analyses were performed on a 594 nt fragment of the ORF1/RdRp region from 84 sinaivirus sequences, including 31 new Lake Sinai viruses (LSVs) from honey bees collected in five countries across the globe and the four ant viral sequences. The phylogeny revealed four main clades potentially representing different viral species infecting honey bees. Moreover, the ant viruses belonged to the LSV4 clade, suggesting a possible cross-species transmission between bees and ants. Lastly, wide honey bee screening showed that all four LSV clades have worldwide distributions with no obvious geographical segregation.


Assuntos
Formigas/virologia , Abelhas/virologia , Vírus de Insetos/classificação , Vírus de Insetos/isolamento & purificação , Filogenia , Animais , Variação Genética , Vírus de Insetos/genética , Análise de Sequência de DNA , Proteínas Virais/genética
17.
J Gen Virol ; 98(9): 2368-2378, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28857036

RESUMO

Solenopsis invicta virus 2 is a single-stranded positive-sense picorna-like RNA virus with an unusual genome structure. The monopartite genome of approximately 11 kb contains four open reading frames in its 5' third, three of which encode proteins with homology to picornavirus-like jelly-roll fold capsid proteins. These are followed by an intergenic region, and then a single long open reading frame that covers the 3' two-thirds of the genome. The polypeptide translation of this 3' open reading frame contains motifs characteristic of picornavirus-like helicase, protease and RNA-dependent RNA polymerase domains. An inspection of public transcriptome shotgun assembly sequences revealed five related apparently nearly complete virus genomes isolated from ant species and one from a dipteran insect. By high-throughput sequencing and in silico assembly of RNA isolated from Solenopsis invicta and four other ant species, followed by targeted Sanger sequencing, we obtained nearly complete genomes for four further viruses in the group. Four further sequences were obtained from a recent large-scale invertebrate virus study. The 15 sequences are highly divergent (pairwise amino acid identities of as low as 17 % in the non-structural polyprotein), but possess the same overall polycistronic genome structure, which is distinct from all other characterized picorna-like viruses. Consequently, we propose the formation of a new virus family, Polycipiviridae, to classify this clade of arthropod-infecting polycistronic picorna-like viruses. We further propose that this family be divided into three genera: Chipolycivirus (2 species), Hupolycivirus (2 species) and Sopolycivirus (11 species), with members of the latter infecting ants in at least 3 different subfamilies.


Assuntos
Formigas/virologia , Picornaviridae/isolamento & purificação , Animais , Genoma Viral , Vírus de Insetos/classificação , Vírus de Insetos/genética , Vírus de Insetos/isolamento & purificação , Filogenia , Picornaviridae/classificação , Picornaviridae/genética , RNA Viral/genética , RNA Viral/metabolismo
18.
Sci Rep ; 7(1): 6925, 2017 07 31.
Artigo em Inglês | MEDLINE | ID: mdl-28761114

RESUMO

Honeybee (Apis mellifera) health is threatened globally by the complex interaction of multiple stressors, including the parasitic mite Varroa destructor and a number of pathogenic viruses. Australia provides a unique opportunity to study this pathogenic viral landscape in the absence of V. destructor. We analysed 1,240A. mellifera colonies across Australia by reverse transcription-polymerase chain reaction (RT-PCR) and next-generation sequencing (NGS). Five viruses were prevalent: black queen cell virus (BQCV), sacbrood virus (SBV), Israeli acute paralysis virus (IAPV) and the Lake Sinai viruses (LSV1 and LSV2), of which the latter three were detected for the first time in Australia. We also showed several viruses were absent in our sampling, including deformed wing virus (DWV) and slow bee paralysis virus (SBPV). Our findings highlight that viruses can be highly prevalent in A. mellifera populations independently of V. destructor. Placing these results in an international context, our results support the hypothesis that the co-pathogenic interaction of V. destructor and DWV is a key driver of increased colony losses, but additional stressors such as pesticides, poor nutrition, etc. may enable more severe and frequent colony losses to occur.


Assuntos
Abelhas/virologia , Vírus de Insetos/classificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Análise de Sequência de RNA/métodos , Animais , Austrália , Abelhas/parasitologia , Dicistroviridae/genética , Dicistroviridae/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , Vírus de Insetos/genética , Vírus de Insetos/isolamento & purificação , Filogenia , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação , Varroidae
19.
Vector Borne Zoonotic Dis ; 17(8): 567-575, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28628366

RESUMO

Culex flavivirus (CxFV) is an insect-specific flavivirus infecting Culex mosquitoes, which are important vectors of West Nile virus (WNV). CxFV and WNV cocirculate in nature and coinfect Culex mosquitoes, including in a WNV "hotspot" in suburban Chicago. We previously identified a positive association between CxFV and WNV in mosquito pools collected from suburban Chicago in 2006. To further investigate this phenomenon, we compared the spatial and temporal distribution of CxFV during an interepidemic year (2011) and an epidemic year (2012) for WNV. Both viruses were more prevalent in mosquito pools in 2012 compared to 2011. During both years, the CxFV infection status of mosquito pools was associated with environmental factors such as habitat type and precipitation frequency rather than coinfection with WNV. These results support the idea that WNV and CxFV are ecologically associated, perhaps because both viruses respond to similar environmental drivers of mosquito populations.


Assuntos
Culex/virologia , Epidemias , Vírus de Insetos/isolamento & purificação , Febre do Nilo Ocidental/epidemiologia , Vírus do Nilo Ocidental/isolamento & purificação , Animais , Chicago/epidemiologia , Fatores de Tempo , Zoonoses
20.
J Gen Virol ; 98(4): 527-528, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28382900

RESUMO

Iflaviridae is a family of small non-enveloped viruses with monopartite, positive-stranded RNA genomes of approximately 9-11 kilobases. Viruses of all classified species infect arthropod hosts, with the majority infecting insects. Both beneficial and pest insects serve as hosts, and infections can be symptomless (Nilaparvatalugens honeydew virus 1) or cause developmental abnormalities (deformed wing virus), behavioural changes (sacbrood virus) and premature mortality (infectious flacherie virus). The host range has not been examined for most members. The most common route of infection for iflaviruses is the ingestion of virus-contaminated food sources. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Iflaviridae, which is available at www.ictv.global/report/iflaviridae.


Assuntos
Vírus de Insetos/classificação , Vírus de RNA/classificação , Animais , Especificidade de Hospedeiro , Vírus de Insetos/genética , Vírus de Insetos/isolamento & purificação , Vírus de Insetos/fisiologia , Insetos/classificação , Insetos/virologia , Filogenia , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação , Vírus de RNA/fisiologia
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