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1.
Acta Cir Bras ; 34(10): e201901005, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31851213

RESUMO

PURPOSE: To quantify and compare the expression of stromal elements in prostate adenocarcinoma of different Gleason scores with non-tumor area (control). METHODS: We obtained 132 specimens from samples of prostate peripheral and transition zone. We analyzed the following elements of the extracellular matrix: collagen fibers, elastic system, smooth muscle fibers and blood vessels. The tumor area and non-tumor area (control) of the TMA (tissue microarray) were photographed and analyzed using the ImageJ software. RESULTS: The comparison between the tumor area and the non-tumor area showed significant differences between stromal prostate elements. There was an increase of collagen fibers in the tumor area, mainly in Gleason 7. Elastic system fibers showed similar result, also from the Gleason 7. Blood vessels showed a significant increase occurred in all analyzed groups. The muscle fibers exhibited a different behavior, with a decrease in relation to the tumor area. CONCLUSIONS: There is a significant difference between the extracellular matrix in prostate cancer compared to the non-tumor area (control) especially in Gleason 7. Important modifications of the prostatic stromal elements strongly correlate with different Gleason scores and can contribute to predict the pathological staging of prostate cancer.


Assuntos
Adenocarcinoma/patologia , Neoplasias da Próstata/patologia , Células Estromais/patologia , Idoso , Idoso de 80 Anos ou mais , Vasos Sanguíneos/patologia , Colágeno/análise , Tecido Elástico/anatomia & histologia , Humanos , Masculino , Pessoa de Meia-Idade , Músculo Liso/patologia , Gradação de Tumores , Valores de Referência , Estudos Retrospectivos , Análise Serial de Tecidos
2.
J Photochem Photobiol B ; 201: 111634, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31715551

RESUMO

Skin Flap is used in reconstructive plastic surgery. However, complications such as ischemia followed by local necrosis may occur, requiring a new surgical procedure. It is well known that photobiomodulation therapy (PBMT) is an effective technique for improving microcirculation and neoangiogenesis, which contributes positively to the blood supply in the pre and post surgical period. Thus, the objective of the present study was to investigate the effects of preemptive treatment with laser PBMT with different energies on the viability in skin flaps in rats. Sixty-three Wistar rats, male, were randomized into five groups: Control Group (CG) (n = 15): PBMT simulation; Preemptive group 1.1 J laser (GP1) (n = 15): preemptive laser PBMT with 1.1 J of energy per point; Preemptive group 4 J laser (GP4) (n = 15): preemptive PBMT with 4 J of energy per point; Laser group 11 J (G1) (n = 9): PBMT immediately after surgery with 1.1 J of energy per point; Laser group 4 J (G4) (n = 9): TFMB immediately after surgery with 4 J of energy per point. The CG, GP1 and GP4 groups started treatment 72 h prior to surgery and were subdivided into two experimental periods, one of them on the day of the flap and the other along with the other groups on the seventh postoperative day. Three days after the randomization, the animals underwent random skin flap surgery. PBMT was performed with a 660 nm laser at three points. In the first experimental period, a greater number of vessels were found, as well as mast cells in GP1 compared to the CG and greater expression of fibroblast growth factor and vascular endothelial growth factor in the GP1 and GP4 groups compared to the CG. In the second experimental period, GP1 presented a lower percentage of necrotic tissue, a higher number of vessels and a percentage of cells labeled with both VEGF and hypoxia indicible factor alpha (HIF-1α) compared to the CG, FGF in GP1, GP4 and G4 when compared to the CG. Thus, it was concluded that preemptive treatment with PBMT with the application of 1.1 J of energy per point is effective in improving the viability of the skin flap.


Assuntos
Lasers Semicondutores , Retalhos Cirúrgicos/patologia , Animais , Vasos Sanguíneos/patologia , Vasos Sanguíneos/efeitos da radiação , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Masculino , Mastócitos/citologia , Mastócitos/metabolismo , Mastócitos/efeitos da radiação , Necrose , Distribuição Aleatória , Ratos , Ratos Wistar , Retalhos Cirúrgicos/irrigação sanguínea , Fator A de Crescimento do Endotélio Vascular/metabolismo
3.
Br J Radiol ; 92(1104): 20181055, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31596129

RESUMO

OBJECTIVE: We proposed to determine whether the performance of inexperienced radiologists in determining extramural vascular invasion (EMVI) in rectal cancer on MRI can be promoted by means of targeted training. METHODS: 230 rectal cancer patients who underwent pre-operative chemoradiotherapy were included. Pre-therapy and post-therapy MR images and pathology EMVI evaluation were available for cases. 230 cases were randomly divided into 150 training cases and 80 testing cases, including 40 testing case A and 40 testing case B. Four radiologists were included for MRI EMVI evaluation, who were divided into targeted training group and non-targeted training group. The two groups evaluated testing case A at baseline, 3 month and 6 month, evaluated testing case B at 6 month. The main outcome was agreement with expert-reference for pre-therapy and post-therapy evaluation, the other outcome was accuracy with pathology for post-therapy evaluation. RESULTS: After 6 months of training, targeted training group showed statistically higher agreement with expert-reference than non-targeted training group for both pre-therapy and post-therapy MRI EMVI evaluation of testing case A and testing case B, all p < 0.05. Targeted training group also showed significantly higher accuracy with pathology than non-targeted training group for post-therapy evaluation of testing case A and testing case B after 6 months of training, all p < 0.05. CONCLUSION: The diagnostic performance for MRI EMVI evaluation could be promoted by targeted training for inexperienced radiologist. ADVANCES IN KNOWLEDGE: This study provided the first evidence that after 6 month targeted training, inexperienced radiologists demonstrated improved diagnostic performance, with a 20% increase in agreement with expert-reference for both pre-therapy and post-therapy MRI EMVI evaluation and also a 20% increase in or accuracy with pathology for post-therapy evaluation, while inexperienced radiologists could not gain obvious improvement in MRI EMVI evaluation through the same period of regular clinical practice. It indicated that targeted training may be necessary for helping inexperienced radiologist to acquire adequate experience for the MRI EMVI evaluation of rectal cancer, especially for radiologist who works in a medical unit where MRI EMVI diagnosis is uncommon.


Assuntos
Competência Clínica , Imagem por Ressonância Magnética , Radiologistas/educação , Neoplasias Retais/diagnóstico por imagem , Neoplasias Retais/patologia , Vasos Sanguíneos/diagnóstico por imagem , Vasos Sanguíneos/patologia , Quimiorradioterapia , Consenso , Endotélio Vascular/diagnóstico por imagem , Endotélio Vascular/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Variações Dependentes do Observador , Cuidados Pós-Operatórios , Cuidados Pré-Operatórios , Radiologistas/normas , Distribuição Aleatória , Neoplasias Retais/irrigação sanguínea , Neoplasias Retais/terapia , Padrões de Referência , Estudos Retrospectivos , Fatores de Tempo
4.
J Appl Oral Sci ; 27: e20180584, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31596366

RESUMO

OBJECTIVES: This study aimed to compare two different soft tissue replacement grafts in their ability to treat gingival recession defects and successfully integrate with the surrounding tissues. METHODOLOGY: Nine beagle dogs were included and followed up to 10 weeks. Sites for intervention were allocated to one of the grafting materials investigated. Treatment consisted of coronally advanced flap combined with one of the two soft tissue substitutes on a previous surgically created defect. Materials employed were porcine-derived acellular dermal matrix (ADM) [Novomatrix™ (Test)] and collagen-based matrix (CBM) [Mucograft® (Control)]. Animals were sacrificed at 2, 6, and 10 weeks postoperatively and compared using descriptive histology and histomorphometric outcomes. RESULTS: Macroscopic findings were similar between test and control groups at all intervals. After 10 weeks, both groups demonstrated successful incorporation of the grafting materials without signs of rejection and with comparable tissue integration. The histomorphometric data were similar between groups at 2 weeks; however, the test group provided greater root coverage and increase in tissue thickness than the control at 6- and 10-weeks post surgically. CONCLUSIONS: Both porcine-derived ADM and CBM revealed similar histological outcomes with successful integration and absence of adverse events. Test group provided superior outcomes regarding root coverage and increase in tissue thickness.


Assuntos
Derme Acelular , Colágeno/uso terapêutico , Retração Gengival/cirurgia , Animais , Vasos Sanguíneos/patologia , Cães , Gengiva/patologia , Gengiva/cirurgia , Retração Gengival/patologia , Teste de Materiais , Valores de Referência , Reprodutibilidade dos Testes , Retalhos Cirúrgicos , Suínos , Fatores de Tempo , Resultado do Tratamento
5.
Invest Ophthalmol Vis Sci ; 60(13): 4328-4335, 2019 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-31622469

RESUMO

Purpose: We evaluate the effect of choroidal vessel density on the residual length of the ellipsoid zone (EZ) and visual function in patients with retinitis pigmentosa (RP) using optical coherence tomography angiography (OCTA). Methods: Fifty-three patients with RP (n = 101 eyes) and 53 normal participants (n = 76 eyes) were enrolled in this study. Patients with RP were assigned to three groups according to their best-corrected visual acuity (BCVA). All patients underwent ophthalmologic examinations, including BCVA, fundus examination performed with a slit-lamp using an indirect 90 diopter (D) lens, OCTA, full-field electroretinogram (ERG), and visual field. The choroidal vessel density in the choriocapillaris-Sattler's layer (DC-S), Haller's layer (DH), horizontal length of the ellipsoid (HEL), and vertical length of the ellipsoid (VEL) were assessed using OCTA and Adobe Photoshop CS3 extended software. Results: A significantly increasing impairment of choroidal vessel density (DC-S and DH) was characterized in the RP groups compared to those of the controls (P < 0.05 for all). The magnitude of the reduction in the DC-S and DH was much easier to identify for more severely impaired BCVA in the RP groups (P < 0.05 for all). The DC-S had the strongest correlation with the HEL, VEL, BCVA, visual field, and b-wave amplitude (r = 0.735, r = 0.753, r = -0.843, r = 0.579, and r = 0.671, respectively). Conclusions: Using noninvasive OCTA, choroidal microcirculation, especially in the small/middle choroidal vessel layers, was a prominent factor affecting the EZ, visual acuity, visual field, and recordable ERG b-wave amplitude of patients with RP. This may provide new insights into the progress mechanism and treatment of RP.


Assuntos
Vasos Sanguíneos/patologia , Corioide/irrigação sanguínea , Retinite Pigmentosa/fisiopatologia , Acuidade Visual/fisiologia , Adulto , Idoso , Vasos Sanguíneos/diagnóstico por imagem , Estudos Transversais , Eletrorretinografia , Feminino , Angiofluoresceinografia , Humanos , Masculino , Pessoa de Meia-Idade , Retina/fisiopatologia , Tomografia de Coerência Óptica/métodos , Campos Visuais/fisiologia , Adulto Jovem
6.
Clin Exp Rheumatol ; 37 Suppl 119(4): 3-14, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31587697

RESUMO

Systemic sclerosis (SSc) is a complex disorder characterised by the involvement of small arteries, microvessels and connective tissue, with deposition of fibrotic tissue and microvascular obliteration in the skin and internal organs. Due to the multifaceted nature of the disease, several articles are published in the medical literature every year, aimed at exploring different aspects of the pathogenesis, internal organ involvement and clinical aspects, and possible therapeutic approaches. In this article we have reviewed the literature on SSc of the past year, with the aim of identifying novel approaches that may help the treating physician in the clinical management of patients.


Assuntos
Microvasos/patologia , Escleroderma Sistêmico , Vasos Sanguíneos/patologia , Fibrose , Humanos , Escleroderma Sistêmico/fisiopatologia , Escleroderma Sistêmico/terapia , Pele
7.
Acta Cir Bras ; 34(8): e201900804, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31618404

RESUMO

PURPOSE: To develop a rabbit model of a short peripheral catheter (SPC) and to observe the effects of different flushing methods on blood vessels. METHODS: Thirty rabbits were randomly divided into three groups (A, B, and C), with ten rabbits per group. In group A, we used pulsed flush; in group B, we used uniform flush; and no treatment was used in group C. RESULTS: We observed that a uniform flush reduced blockage, phlebitis, and exudation compared to a pulsed flush by visual observation. The histopathological examination found that the morphological changes in group A were more severe than in group B and C related to loss of venous endothelial cells, inflammatory cell infiltration, edema, epidermal and chondrocyte degeneration, except for the thrombosis on group B that was more serious than in group A, especially in the distal side of puncture points. The distal region of groups A and B had more inflammatory cell infiltration than the proximal region. Thrombosis was more severe in the distal region than in the proximal region in group B. CONCLUSIONS: The uniform flush produced less damage to the vascular endothelium and surrounding tissues and was superior to the pulsed flush. However, the uniform flush is prone to thrombosis.


Assuntos
Vasos Sanguíneos/patologia , Cateterismo Periférico/métodos , Animais , Cateterismo Periférico/efeitos adversos , Modelos Animais de Doenças , Orelha/irrigação sanguínea , Células Endoteliais , Endotélio Vascular/patologia , Endotélio Vascular/ultraestrutura , Masculino , Flebite/etiologia , Coelhos , Distribuição Aleatória , Fluxo Sanguíneo Regional
8.
Indian J Med Res ; 149(4): 503-507, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-31411174

RESUMO

Background & objectives: There is a paucity of literature on the cognitive profiles of vascular dementia (VaD) in India. The current study was undertaken to investigate the pattern of cognitive deficits in patients with VaD. Methods: Fifty patients fulfilling the Diagnostic and Statistical Manual of Mental Disorders-IV criteria of dementia and National Institute of Neurological Disorders and Stroke - Association Internationale pour la Recherche et l'Enseignement en Neurosciences criteria for VaD were assessed using Mini Mental State Examination, Kolkata Cognitive Screening Battery and other relevant tests including magnetic resonance imaging of brain. Results: Twenty patients had small vessel dementia, whereas the least common was haemorrhagic dementia in four patients. In patients with small vessel dementia, apart from memory, all patients had problem in attention and executive function, whereas 12 patients had visuoconstructional deficit and eight patients had language problem. In a total of 12 patients with large vessel dementia, apart from memory, executive dysfunction and visuoconstructional deficit were noted in 10 patients, whereas attention deficit was noted in eight patients. Attention was found to be more involved in small-vessel dementia than large-vessel dementia though all had memory impairment (P<0.01). Interpretation & conclusions: Small vessel dementia was the commonest subtype of VaD in our study. Memory, attention and executive functions were predominantly affected in patients with VaD. Attention was significantly more involved in small vessel dementia than large vessel dementia. Further studies with large sample size need to be done in different regions of the country.


Assuntos
Vasos Sanguíneos/diagnóstico por imagem , Transtornos Cognitivos/fisiopatologia , Disfunção Cognitiva/fisiopatologia , Demência Vascular/fisiopatologia , Idoso , Vasos Sanguíneos/patologia , Encéfalo/fisiopatologia , Transtornos Cognitivos/complicações , Transtornos Cognitivos/diagnóstico por imagem , Disfunção Cognitiva/complicações , Disfunção Cognitiva/diagnóstico por imagem , Demência Vascular/complicações , Demência Vascular/diagnóstico por imagem , Feminino , Humanos , Índia/epidemiologia , Imagem por Ressonância Magnética , Masculino , Pessoa de Meia-Idade
10.
Clin Appl Thromb Hemost ; 25: 1076029619862052, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31298044

RESUMO

Hemophilic arthropathy from joint bleeding remains a complication with major morbidity in the increasingly aging patients with hemophilia. Prophylactic clotting factor infusions, based on pharmacokinetic dosing to reduce bleeding rates, are being explored more and more. However, there is little evidence on the benefits of pharmacokinetic dosing in direct association with bleeding events. Here, we prospectively followed a cohort of adult patients with hemophilia A and B (n = 26) and arthropathic joints on various clotting factor products over a period of 2 years with clinical and radiographic joint health assessments, frequent joint ultrasound, and pharmacokinetic studies. Joint bleeds and synovitis with synovial vascularity changes were objectively diagnosed by musculoskeletal ultrasound and power Doppler and analyzed in relation to pharmacokinetic, joint- and patient-specific parameters. Results revealed that, contrary to common beliefs, bleeding episodes were not readily explained by pharmacokinetic features, as they were not associated with more time spent below certain clotting factor thresholds. Joint bleeding was found to be associated with prominent vascularity changes, suggesting that vascular remodeling and leakiness may contribute to joint bleeding that cannot be prevented by clotting factor replacement alone.


Assuntos
Fatores de Coagulação Sanguínea/farmacocinética , Vasos Sanguíneos/patologia , Hemartrose/etiologia , Hemartrose/prevenção & controle , Hemofilia A/complicações , Hemofilia B/complicações , Adulto , Fatores de Coagulação Sanguínea/análise , Fatores de Coagulação Sanguínea/uso terapêutico , Vasos Sanguíneos/diagnóstico por imagem , Fragilidade Capilar , Hemartrose/diagnóstico por imagem , Hemartrose/tratamento farmacológico , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Ultrassonografia/métodos , Remodelação Vascular
11.
Int J Mol Sci ; 20(15)2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31344780

RESUMO

Collaterals are unique blood vessels present in the microcirculation of most tissues that, by cross-connecting a small fraction of the outer branches of adjacent arterial trees, provide alternate routes of perfusion. However, collaterals are especially susceptible to rarefaction caused by aging, other vascular risk factors, and mouse models of Alzheimer's disease-a vulnerability attributed to the disturbed hemodynamic environment in the watershed regions where they reside. We examined the hypothesis that endothelial and smooth muscle cells (ECs and SMCs, respectively) of collaterals have specializations, distinct from those of similarly-sized nearby distal-most arterioles (DMAs) that maintain collateral integrity despite their continuous exposure to low and oscillatory/disturbed shear stress, high wall stress, and low blood oxygen. Examination of mouse brain revealed the following: Unlike the pro-inflammatory cobble-stoned morphology of ECs exposed to low/oscillatory shear stress elsewhere in the vasculature, collateral ECs are aligned with the vessel axis. Primary cilia, which sense shear stress, are present, unexpectedly, on ECs of collaterals and DMAs but are less abundant on collaterals. Unlike DMAs, collaterals are continuously invested with SMCs, have increased expression of Pycard, Ki67, Pdgfb, Angpt2, Dll4, Ephrinb2, and eNOS, and maintain expression of Klf2/4. Collaterals lack tortuosity when first formed during development, but tortuosity becomes evident within days after birth, progresses through middle age, and then declines-results consistent with the concept that collateral wall cells have a higher turnover rate than DMAs that favors proliferative senescence and collateral rarefaction. In conclusion, endothelial and SMCs of collaterals have morphologic and functional differences from those of nearby similarly sized arterioles. Future studies are required to determine if they represent specializations that counterbalance the disturbed hemodynamic, pro-inflammatory, and pro-proliferative environment in which collaterals reside and thus mitigate their risk factor-induced rarefaction.


Assuntos
Vasos Sanguíneos/metabolismo , Circulação Colateral/genética , Miócitos de Músculo Liso/metabolismo , Neovascularização Fisiológica/genética , Envelhecimento/genética , Envelhecimento/metabolismo , Doença de Alzheimer/genética , Doença de Alzheimer/metabolismo , Animais , Vasos Sanguíneos/patologia , Circulação Colateral/fisiologia , Células Endoteliais/metabolismo , Artéria Femoral/crescimento & desenvolvimento , Artéria Femoral/metabolismo , Membro Posterior/irrigação sanguínea , Humanos , Camundongos , Fatores de Risco , Transdução de Sinais
12.
Immunity ; 51(1): 90-103.e3, 2019 07 16.
Artigo em Inglês | MEDLINE | ID: mdl-31278057

RESUMO

The key sites within the gastrointestinal (GI) tract where T cells mediate effector responses and the impact of these responses on intestinal stem cells (ISCs) remain unclear. Using experimental bone marrow transplantation to model immune-mediated GI damage and 3D imaging to analyze T cell localization, we found that the ISC compartment is the primary intestinal site targeted by T cells after transplantation. Recruitment to the crypt base region resulted in direct T cell engagement with the stem cell compartment and loss of crypt base columnar ISCs, which expressed both MHC classes I and II. Vasculature expressing the adhesion molecule MAdCAM-1 clustered near the crypt base, preferentially regulating crypt compartment invasion and ISC reduction without affecting T cell migration to villi. These findings indicate that allogeneic T cells rapidly access the stem cell niche after transplantation, and this targeted recruitment to the stem cell compartment results in ISC loss during immune-mediated GI damage.


Assuntos
Células-Tronco Adultas/imunologia , Transplante de Medula Óssea , Mucosa Intestinal/imunologia , Nicho de Células-Tronco/imunologia , Linfócitos T/imunologia , Animais , Vasos Sanguíneos/metabolismo , Vasos Sanguíneos/patologia , Moléculas de Adesão Celular/metabolismo , Movimento Celular , Citotoxicidade Imunológica , Feminino , Humanos , Imagem Tridimensional , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Pessoa de Meia-Idade , Modelos Animais , Transplante Homólogo
13.
Cardiovasc Pathol ; 42: 44-53, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31255975

RESUMO

This article introduces the Second Special Issue of Cardiovascular Pathology (CVP), the official journal of the Society for Cardiovascular Pathology (SCVP). This CVP Special Issue showcases a series of commemorative review articles in celebration of the 25th anniversary of CVP originally published in 2016 and now compiled into a virtual collection with online access for the cardiovascular pathology community. This overview also provides updates on the major categories of cardiovascular diseases from the perspective of cardiovascular pathologists, highlighting publications from CVP, as well as additional important review articles and clinicopathologic references.


Assuntos
Vasos Sanguíneos/patologia , Doenças Cardiovasculares/patologia , Miocárdio/patologia , Doenças Cardiovasculares/mortalidade , Doenças Cardiovasculares/terapia , Causas de Morte , Humanos , Prognóstico , Fatores de Risco
14.
Eur. j. anat ; 23(4): 267-272, jul. 2019. ilus
Artigo em Inglês | IBECS | ID: ibc-183000

RESUMO

Ayurvedic medicines is known to use heavy metals in their preparation. Nagabhasma is one such form of a lead-based medicine. Even though lead is known to be toxic to several systems of the human body, according to Ayurveda, the metallic toxicity of the lead gets nullified and thereby imbibes medicinal property when it is prepared using many herbs and stringent traditional methods. Therefore, the present study is designed to evaluate the effect of such detoxified lead in various stages of authentically prepared Nagabhasma on the histopathology of liver in comparison with lead acetate and commercially available Nagabhasma-administered animals. Less than the human-equivalent doses of Nagabhasma at four intermittent stages of its preparation were administered orally for 30 days and 60 days (short term and long term exposure) to Wistar rats. In another set of experiment, test-material-administered animals were kept under observation for an additional period of two months to record the residual effect. Immediately after the administration and after the observation period, the animals were sacrificed to collect the liver for histopathological examination. The histopathological results of the immediate and residual effects showed varying alterations in the microarchitecture of the liver as the stages of Nagabhasma preparation advanced. The final product (stage 4 bhasma), showed very less toxic effect in comparison with other stages. In conclusion, the results state that, by following the traditional procedures while preparing Nagabhasma, the metallic lead gets converted into non-toxic organometallic compound


No disponible


Assuntos
Humanos , Animais , Metais Pesados/toxicidade , Medicina Ayurvédica/efeitos adversos , Fígado/efeitos dos fármacos , Chumbo/toxicidade , Hepatócitos/efeitos dos fármacos , Capilares/efeitos dos fármacos , Fígado/anatomia & histologia , Fígado/patologia , Ratos Wistar , Vasos Sanguíneos/anatomia & histologia , Vasos Sanguíneos/patologia
15.
J Mater Sci Mater Med ; 30(6): 67, 2019 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-31165278

RESUMO

Braiding of Nitinol micro wires is an established technology for the manufacturing of fine-meshed neurovascular implants for tortuous vessel geometries. Electropolishing of wires before the braiding process has the potential to improve the in vitro behaviour in terms of thrombogenicity and endothelial cell proliferation. In this study, we present the first in vitro investigation of braided electropolished/blue oxide Nitinol samples in a blood flow loop, showing a significantly lower activation of the coagulation pathway (represented by the TAT III marker) and a tendency towards reduced platelet adhesion. Furthermore, we applied the same surface treatment on flat disks and measured protein adhesion as well as endothelial cell proliferation. We compared our results to non-electropolished samples with a native oxide surface. While platelet deposition was reduced on electropolished/blue oxide surface, a significant increase of endothelial cell seeding was observed. Investigation of inflammatory marker expression in endothelial cells provided divergent results depending on the marker tested, demanding closer investigation. Surface analysis using Auger electron spectroscopy revealed a thin layer mainly consisting of titanium oxynitride or titanium oxide + titanium nitride as a potential cause of the improved biological performance. Translated to the clinical field of intracranial aneurysm treatment, the improved biocompatibility has the potential to increase both safety (low thrombogenicity) and effectiveness (aneurysm neck reconstruction).


Assuntos
Ligas/química , Coagulação Sanguínea/efeitos dos fármacos , Vasos Sanguíneos/patologia , Materiais Revestidos Biocompatíveis/química , Células Endoteliais/citologia , Adesividade Plaquetária , Próteses e Implantes , Adsorção , Aneurisma/cirurgia , Plaquetas , Adesão Celular , Proliferação de Células , Elasticidade , Eletroquímica , Humanos , Inflamação , Teste de Materiais , Níquel/química , Óxidos/química , Segurança do Paciente , Propriedades de Superfície , Titânio/química
16.
Sud Med Ekspert ; 62(3): 9-11, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31198197

RESUMO

Diagnosis of sudden death in the young is based on the results of a set of research methods. Immunohistochemical, histochemical and histochemical methods are fundamental to the diagnosis of hidden forms of connective tissue pathology. These methods allow the sequence of initial and irreversible changes in connective tissue structures to be determined. The aim of this study was to determine the abnormalities of the vascular wall structure when using immunohistochemical and histological methods for the verification of congenital anomalies of various types of vessels, which expand the criteria for the diagnostic detection of individuals with connective tissue dysplasia. These investigation methods allow the diagnosis of hidden forms of congenital connective tissue pathology in the young. The main morphological signs of pathology of the aorta, carotid arteries, coronary and cerebral arteries are the disturbance of structural elements of the connective tissue.


Assuntos
Vasos Sanguíneos/patologia , Tecido Conjuntivo/patologia , Morte Súbita , Humanos
17.
Rev Cardiovasc Med ; 20(1): 19-25, 2019 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31184092

RESUMO

Vascular aging is a major risk factor and driver of agerelated cardiovascular diseases (CVD). Atherosclerosis, hypertension, and other CVD lead to vascular dysfunction that involves multiple pathological processes such as oxidative stress, endothelial dysfunction, inflammation, and autophagy. Epigenetics refers to genetic changes that occur when the DNA remains unchanged that include DNA methylation, histone modification, and non-coding RNA. It has been reported that epigenetics plays an effective regulatory role in CVD and affects cardiovascular repair function. Presently, drugs targeting epigenetics have applications in malignant tumors and inflammation. Therefore, exploration of epigenetic mechanisms in vascular aging will allow us to understand the pathogenesis of diseases related to vascular aging. This review focuses on the pathological changes in vascular aging and analyzes the relationship between vascular aging and epigenetics. Additionally, this review focuses on the pathogenesis of vascular aging related diseases from a new perspective in order to develop epigenetic-based treatment strategies for patients with age-related cardiovascular diseases.


Assuntos
Envelhecimento/genética , Vasos Sanguíneos/metabolismo , Doenças Cardiovasculares/genética , Epigênese Genética , Fatores Etários , Envelhecimento/patologia , Animais , Vasos Sanguíneos/efeitos dos fármacos , Vasos Sanguíneos/patologia , Vasos Sanguíneos/fisiopatologia , Fármacos Cardiovasculares/uso terapêutico , Doenças Cardiovasculares/tratamento farmacológico , Doenças Cardiovasculares/metabolismo , Doenças Cardiovasculares/patologia , Montagem e Desmontagem da Cromatina , Metilação de DNA , Epigênese Genética/efeitos dos fármacos , Hemodinâmica , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Transdução de Sinais , Remodelação Vascular
18.
Oxid Med Cell Longev ; 2019: 5953685, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31214280

RESUMO

Type 2 diabetes (T2DM) and its complications constitute a major worldwide public health problem, with high rates of morbidity and mortality. Biomarkers for predicting the occurrence and development of the disease may therefore offer benefits in terms of early diagnosis and intervention. This review provides an overview of human studies on circulating biomarkers of oxidative stress and antioxidant defence systems and discusses their usefulness from a clinical perspective. Most case-control studies documented an increase in biomarkers of oxidative lipid, protein, and nucleic acid damage in patients with prediabetes and in those with a diagnosis of T2DM compared to controls, and similar findings were reported in T2DM with micro- and macrovascular complications compared to those without. The inconsistence of the results regarding antioxidant defence systems renders difficulty to draw a general conclusion. The clinical relevance of biomarkers of oxidative lipid and protein damage for T2DM progression is uncertain, but prospective studies suggest that markers of oxidative nucleic acid damage such as 8-hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine are promising for predicting macrovascular complications of T2DM. Emerging evidence also points out the relationship between serum PON1 and serum HO1 in T2DM and its complications. Overall, enhanced oxidative damage represents an underlying mechanism of glucose toxicity in T2DM and its related micro- and macrovascular complications suggesting that it may be considered as a potential additional target for pharmacotherapy. Therefore, further studies are needed to understand whether targeting oxidative stress may yield clinical benefits. In this view, the measurement of oxidative stress biomarkers in clinical trials deserves to be considered as an additional tool to currently used parameters to facilitate a more individualized treatment of T2DM in terms of drug choice and patient selection.


Assuntos
Biomarcadores/sangue , Vasos Sanguíneos/metabolismo , Complicações do Diabetes/diagnóstico , Diabetes Mellitus Tipo 2/diagnóstico , /sangue , Arildialquilfosfatase/sangue , Vasos Sanguíneos/patologia , Ensaios Clínicos como Assunto , Dano ao DNA , Heme Oxigenase-1/sangue , Humanos , Metabolismo dos Lipídeos , Estresse Oxidativo
19.
Lipids Health Dis ; 18(1): 125, 2019 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-31138221

RESUMO

BACKGROUND: Exercise has proved effective in attenuating the unfavourable response normally associated with postprandial hypertriglyceridemia (PHTG) and accompanying oxidative stress. Yet, the acute effects of prior exercise and PHTG on DNA damage remains unknown. The purpose of this study was to examine if walking alters PHTG-induced oxidative damage and the interrelated inflammatory mechanisms. METHODS: Twelve apparently healthy, recreationally active, male participants (22.4 ± 4.1 years; 179.2 ± 6 cm; 84.2 ± 14.7 kg; 51.3 ± 8.6 ml·kg- 1·min- 1) completed a randomised, crossover study consisting of two trials: (1) a high-fat meal alone (resting control) or (2) a high-fat meal immediately following 1 h of moderate exercise (65% maximal heart rate). Venous blood samples were collected at baseline, immediately post-exercise or rest, as well as at 2, 4 and 6 h post-meal. Biomarkers of oxidative damage (DNA single-strand breaks, lipid peroxidation and free radical metabolism) and inflammation were determined using conventional biochemistry techniques. RESULTS: DNA damage, lipid peroxidation, free radical metabolism and triglycerides increased postprandially (main effect for time, p < 0.05), regardless of completing 1 h of preceding moderate intensity exercise. Plasma antioxidants (α-tocopherol and γ-tocopherol) also mobilised in response to the high-fat meal (main effect for time, p < 0.05), but no changes were detected for retinol-binding protein-4. CONCLUSION: The ingestion of a high fat meal induces postprandial oxidative stress, inflammation and a rise in DNA damage that remains unaltered by one hour of preceding exercise.


Assuntos
Vasos Sanguíneos/patologia , Dano ao DNA , Exercício/fisiologia , Hipertrigliceridemia/sangue , Inflamação/sangue , Linfócitos/patologia , Período Pós-Prandial , Antioxidantes/metabolismo , Biomarcadores/metabolismo , Sedimentação Sanguínea , Quebras de DNA de Cadeia Simples , Humanos , Peróxidos Lipídicos/metabolismo , Masculino , Solubilidade , Adulto Jovem
20.
Mol Med Rep ; 20(1): 205-215, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31115522

RESUMO

Tendon adhesion is a substantial challenge for tendon repair. Thermal pretreatment (TP) may decrease inflammation by upregulating heat shock proteins (HSPs). The present study intends to identify the function that TP serves when combined with HSP70 overexpression in tendon healing and adhesion in rats. Sprague­Dawley male rats were used to establish a surgically ablative tendon postoperative suture model, and the positive expression of the HSP70 protein was measured using immunohistochemistry. Changes to the blood vessels and collagenous fiber, in addition to the maximum tensile strength and the tendon sliding distance, were detected under a microscope. Finally, HSP70, tumor growth factor ß (TGF­ß), and insulin­like growth factor 1 (IGF­1) mRNA and protein levels were all determined by employing reverse transcription­quantitative polymerase chain reaction and western blot analysis methods. The positive expression of the HSP70 protein increased following TP. Furthermore, TP reduced the infiltration of inflammatory cells and improved the collagenous arrangement, accompanied by an increased maximum tensile force and tendon gliding distance following surgery. In addition, TP increased the mRNA and protein expression levels of HSP70, TGF­ß and IGF­1. Altogether, TP increases HSP70 expression, thereby reducing postoperative traumatic inflammation and establishing tendon adhesion and promoting tendon healing. Thus, TP may be a potential strategy for the treatment of tendon adhesion.


Assuntos
Proteínas de Choque Térmico HSP70/genética , Tendões/cirurgia , Aderências Teciduais/terapia , Animais , Vasos Sanguíneos/metabolismo , Vasos Sanguíneos/patologia , Regulação da Expressão Gênica/genética , Temperatura Alta/uso terapêutico , Fator de Crescimento Insulin-Like I/genética , Masculino , RNA Mensageiro/genética , Ratos , Técnicas de Sutura , Tendões/metabolismo , Tendões/patologia , Resistência à Tração , Aderências Teciduais/genética , Aderências Teciduais/patologia , Fator de Crescimento Transformador beta/genética
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