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1.
Int J Syst Evol Microbiol ; 70(1): 172-179, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31622230

RESUMO

Two Gram-stain-negative, catalase- and oxidase-positive, facultative anaerobic and rod-shaped motile bacteria, designated strains BEI176T and BEI207T, were isolated from seawater collected in the East China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains BEI176T and BEI207T belonged to the genus Vibrio and were closely related to each other with 98.18 % similarity. The closest phylogenetic relatives of strain BEI176T were Vibrio alginolyticus LMG 4409T (98.85 %) and Vibrio campbellii LMG 11216T (98.81 %), whereas the closest relative of strain BEI207T was Vibrio hepatarius LMG 20362T (98.64 %). The two strains showed growth at different conditions; while strain BEI176T grew at 16-37 °C, pH 5.0-9.5 and 0-7.0 % (w/v) NaCl, the growth of strain BEI207T occurred at 10-37 °C, pH 6.0-9.5 and 1.0-7.0 % (w/v) NaCl. Both strains shared the same major fatty acid components of summed feature 3 (C16 : 1ω7c or C16 : 1ω6c), C16 : 0 and summed feature 8 (C18 : 1ω6c or C18 : 1ω7c). The DNA G+C contents of the assembled genomic sequences were 44.73 and 45.06 mol% for strains BEI176T and BEI207T, respectively. Average nucleotide identity values between the two strains and their reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the two strains had less than 70 % similarity to their relatives. Therefore, two novel Vibrio species are proposed to accommodate them: Vibrioouci sp. nov. (type strain, BEI176T=MCCC 1K03515T=JCM 32690T= KCTC 62616T) and Vibrioaquaticus sp. nov. (type strain, BEI207T=MCCC 1K03516T=JCM 32691T=KCTC 62617T).


Assuntos
Filogenia , Água do Mar/microbiologia , Vibrio/classificação , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vibrio/isolamento & purificação
2.
Mar Pollut Bull ; 149: 110548, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31550576

RESUMO

The present study is aimed to monitoring the ecological indicator and pathogenic microorganism diversity in pilgrimage places beach sand on the Bay of Bengal coast. The samples were collected from three locations and four different sites, and were analyzed by following standard methods. The results clearly indicates, ritual activities were highly contaminated in the beach sand qualities, and exceeded with the standard permissible limit of WHO, USEPA, EU, CPCB beach sand recreational and other contacts activities including pH (11%), TBC (100%), TCB (97%), FCB (88%), TEB (75%), E. coli (75%), disease-causing possible level of Klebsiella (84%), Shigella (75%), Salmonella (63%) and Vibrio (56%). The statistical tools were applied to find the strong evidence. The current study pointed out the major effects on the diffusion of potentially pathogenic microorganisms along the shoreline provided useful information for the setup of measures for public health protection in the Bay of Bengal coast.


Assuntos
Praias , Baías/microbiologia , Comportamento Ritualístico , /métodos , Enterobacteriaceae/isolamento & purificação , Escherichia coli/isolamento & purificação , Humanos , Índia , Klebsiella/isolamento & purificação , Salmonella/isolamento & purificação , Shigella/isolamento & purificação , Vibrio/isolamento & purificação , Microbiologia da Água
3.
Mar Pollut Bull ; 149: 110570, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31542593

RESUMO

Microbial safety of recreational waters is a significant public health issue. In this study we assessed the occurrence and quantity of enteric viruses in bathing and non-bathing waters in Italy, in parallel with microbial faecal indicators, somatic coliphages and Vibrio spp. Enteric viruses (aichivirus, norovirus and enterovirus) were detected in 55% of bathing water samples, including samples with bacterial indicator concentrations compliant with the European bathing water Directive. Aichivirus was the most frequent and abundant virus. Adenovirus was detected only in non-bathing waters. Somatic coliphages were identified in 50% bathing water samples, 80% of which showed simultaneous presence of viruses. Vibrio species were ubiquitous, with 9 species identified, including potential pathogens (V. cholerae, V. parahaemoylticus and V. vulnificus). This is the first study showing the occurrence and high concentration of Aichivirus in bathing waters and provides original information, useful in view of a future revision of the European Directive.


Assuntos
Praias , Água do Mar/microbiologia , Água do Mar/virologia , Colífagos , Enterovirus , Monitoramento Ambiental , Fezes/microbiologia , Fezes/virologia , Humanos , Itália , Mar Mediterrâneo , Norovirus/genética , Norovirus/isolamento & purificação , Vibrio/genética , Vibrio/isolamento & purificação , Vibrio/patogenicidade , Microbiologia da Água
4.
Mar Pollut Bull ; 149: 110559, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31543492

RESUMO

To reduce the outbreaks caused by the major pathogenic Vibrio species, V. parahaemolyticus, V. vulnificus, and V. cholerae, the distribution, antibiotic resistance, and virulence of these Vibrio strains were monitored in shellfish and seawater along the Korean coast. Among the Vibrio strains, V. parahaemolyticus was the most abundant species; during summer, this strain showed a substantial increase that correlated with the water temperature. Although >99.0% of the Vibrio species isolates were sensitive to seven antimicrobials recommended by the Center for Disease Control and Prevention for the treatment of Vibrio infections, multiple-antibiotic resistance to at least three antimicrobials was found in 14.3% to 50.0% of each Vibrio species. Among V. parahaemolyticus isolates, 14.3% were positive for the trh gene, whereas only 1% was positive for the tdh gene. These results should aid in implementing proper precautions to avoid potential human health risks associated with exposure to pathogenic Vibrio species.


Assuntos
Aquicultura , Monitoramento Ambiental , Alimentos Marinhos/microbiologia , Frutos do Mar/microbiologia , Vibrio/isolamento & purificação , Animais , Antibacterianos/farmacologia , Farmacorresistência Bacteriana , República da Coreia , Água do Mar/microbiologia , Vibrio/efeitos dos fármacos , Vibrio/patogenicidade , Virulência
5.
J Microbiol ; 57(9): 759-768, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31376108

RESUMO

The cultivation of microbial species remains a primary challenge in microbiology and obtaining pure cultures is essential for the study of microbial physiology and function. When isolating microorganisms from aquaculture environments, Vibrio are the most dominate isolates on the media that are commonly used. In order to expand our ability to study microbial species, an easy-operation and low-cost medium that can reduce the interference of Vibrio strains and increase the cultivability of other bacteria is urgently needed. We compared viable cell counts on conventional media (CM; including Marine Agar 2216 and LB media) and diluted media (DM; including 1/10-Marine Agar 2216, 1/10-LB). We also assessed the diversity of cultivable microorganisms under high and low nutrient conditions by a plate-wash strategy coupled with high-throughput sequencing of the V4 hypervariable region of the 16S rRNA gene. The results show that microbial communities from DM, especially 1/10-Marine Agar 2216, are more diverse than those obtained from CM. Vibrio isolates were reduced on DM. PICRUSt analysis revealed that nutrient composition is a significant contributor to the diversity and function of the cultivable microbial communities. Bacteria grown on CM possess more pathogenic characteristics, whereas DM favors the growth of bacteria that have multiple metabolic functions. Collectively, our data provide strong evidence that dilution of CM influences the cultivability of bacteria from aquaculture seawater. It also supports that DM can expand the range of microbial species that can be cultivated. This study also provides insights for media design in microbial cultivation from aquaculture systems.


Assuntos
Meios de Cultura/metabolismo , Água do Mar/microbiologia , Vibrio/crescimento & desenvolvimento , Bactérias/classificação , Bactérias/genética , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , Meios de Cultura/química , DNA Bacteriano/genética , Filogenia , RNA Ribossômico 16S/genética , Vibrio/genética , Vibrio/isolamento & purificação , Vibrio/metabolismo
6.
Antonie Van Leeuwenhoek ; 112(11): 1603-1610, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31172330

RESUMO

A Gram-stain negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP187T, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain TP187T is related to members of the genus Vibrio and has high 16S rRNA gene sequence similarity with the type strains of Vibrio chagasii (97.3%) and Vibrio gallaecicus (97.1%). Sequence similarities to all other type strains of current species of the genus Vibrio were below 97%. The polar lipids profile was found to contain diphosphatidylglycerol, phosphatidylglycerol, an aminophospholipid, two aminolipids, four phospholipids and eleven unidentified polar lipids. Ubiquinone Q-8 was detected as the predominant quinone. The genomic DNA G + C content of strain TP187T was determined to be 43.7 mol%. In addition, the maximum values of in silico DNA-DNA hybridization (isDDH) and average nucleotide identity (ANI) between strain TP187T with V. chagasii LMG 21353T were 22.40 and 77.50% respectively. Both values are below the proposed cutoff levels for species delineation, i.e. 70 and 95%, respectively. Combined data from phenotypic, phylogenetic, isDDH and ANI data demonstrated that the strain TP187T is representative of a novel species of the genus Vibrio, for which we propose the name Vibrio profundi sp. nov. (type strain TP187T = KACC 18555T = CGMCC 1.15395T).


Assuntos
Sedimentos Geológicos/microbiologia , Água do Mar/microbiologia , Vibrio/classificação , Genoma Bacteriano , Tipagem de Sequências Multilocus , Oceano Pacífico , Fenótipo , Filogenia , Vibrio/química , Vibrio/isolamento & purificação , Sequenciamento Completo do Genoma
7.
Int J Syst Evol Microbiol ; 69(8): 2277-2282, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31125303

RESUMO

A Gram-stain-negative, facultatively anaerobic Vibrio strain, designated NFH.MB010T, was isolated from an epidermal lesion on the test (hard shell skeleton) of a green sea urchin (Strongylocentrotus droebachiensis) collected from northern Norway. Cells of strain NFH.MB010T were rod shaped and motile by means of a single, long polar flagellum. Growth was observed at 1-5% NaCl (w/v) and at 4 °C, but not above 28 °C. Phylogenetic analyses based on eight-gene multilocus sequence analysis (16S rRNA, atpA, gyrB, mreB, pyrH, recA, rpoA and rpoD) suggested novelty at the species level. In silico DNA-DNA hybridization and orthologous average nucleotide identity estimates showed percentage genomic resemblances to its closest relative, Vibrio splendidus, that were well below the established same species threshold values. Phenotypically, utilization of glycogen and gentiobiose, inability of acetoin production, and undetectable valine arylamidase and trypsin activity discriminated strain NFH.MB010T from the closely related reference strains. Protein spectra generated by maldi-tof mass spectrometry further consolidated the species level uniqueness of strain NFH.MB010T. Based on the described polyphasic approach, strain NFH.MB010T therefore appears as a novel species within the Splendidus clade of the genus Vibrio, and the name Vibrio echinoideorum sp. nov. is proposed, with NFH.MB010T (=DSM 107264T=LMG 30656T) as the type strain.


Assuntos
Filogenia , Strongylocentrotus/microbiologia , Vibrio/classificação , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Genes Bacterianos , Tipagem de Sequências Multilocus , Noruega , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vibrio/isolamento & purificação
8.
Int Microbiol ; 22(4): 501-509, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31098824

RESUMO

Singleplex and duplex loop-mediated isothermal amplification (LAMP) assays were developed for detecting Vibrio anguillarum, a major bacterial pathogen of fish, and Vibrio alginolyticus, a pathogen of fish and humans, separately and simultaneously from contaminated seawater by targeting the groEL gene of V. anguillarum, which encodes a molecular chaperone protein, and the fklB gene of V. alginolyticus, which encodes a 22 kilodalton (kDa) peptidyl prolyl isomerase. The optimal reaction conditions to produce consistent results were 65 °C for 30 min, 63 °C for 30 min, and 63 °C for 40 min for the groEL (singleplex for V. anguillarum), fklB (singleplex for V. alginolyticus), and groEL + flkB (duplex) LAMP assays, respectively, analyzed via visual detection methods (use of calcein, and SYBR Green I) and agarose gel electrophoresis. The assays were found to be species-specific, as closely related Vibrio spp. were not detected. The limits of detection (LoDs) of the LAMP assays for DNA template from pure culture and artificially contaminated seawater were 10 and 14 fg (groEL assay; for V. anguillarum), 12.5 and 17 fg (fklB assay; for V. alginolyticus), and 50 and 70 fg (duplex assay) per reaction, respectively, which were much better than the LoDs of conventional polymerase chain reaction (PCR). Singleplex and duplex LAMP assays were found to be rapid, species-specific, and sensitive for the detection of V. anguillarum and V. alginolyticus and are applicable to laboratory and field diagnostics.


Assuntos
Proteínas de Bactérias/genética , Chaperonina 60/genética , Doenças dos Peixes/microbiologia , Técnicas de Amplificação de Ácido Nucleico/métodos , Vibrioses/microbiologia , Vibrioses/veterinária , Vibrio/isolamento & purificação , Animais , Doenças dos Peixes/diagnóstico , Humanos , Vibrio/genética , Vibrioses/diagnóstico , Vibrio alginolyticus/classificação , Vibrio alginolyticus/genética
9.
PLoS One ; 14(4): e0215254, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31013284

RESUMO

A decade long study was conducted to investigate the ecological, biological, and temporal conditions that affect concentrations of Vibrio spp. bacteria in a well-studied lagoonal estuary. Water samples collected from the Neuse River Estuary in eastern North Carolina from 2004-2014 (with additional follow-up samples from Fall of 2018) were analyzed to determine Vibrio spp. concentrations, as well as the concentrations of inorganic and organic nutrients, fecal indicator bacteria, phytoplankton biomass, and a wide range of other physio-chemical estuarine parameters. A significant increase in Vibrio spp. was observed to occur in the estuary over the examined period. Strikingly, over this long duration study period, this statistically significant increase in total culturable Vibrio spp. concentrations does not appear to be correlated with changes in salinity, temperature, or dissolved oxygen, the three most commonly cited influential factors that predict estuarine Vibrio spp. abundance. Furthermore, shorter term (~3 years) data on specific Vibrio species (V. vulnificus and V. parahaemolyticus)show that while Vibrio spp. are increasing overall as a genus, the numbers of some key potentially pathogenic species are decreasing as a part of the total population, further supporting the concept that quantification of the entire genus is not a worthwhile use of resources toward predicting levels of specific potentially pathogenic species of public health concern. The significant increase in this concentration of Vibrio spp. in the studied estuary appears to be related to nitrogen and carbon in the system, indicating a continued need for further research.


Assuntos
Monitorização de Parâmetros Ecológicos/estatística & dados numéricos , Estuários/estatística & dados numéricos , Rios/microbiologia , Vibrio/isolamento & purificação , North Carolina
10.
Int J Syst Evol Microbiol ; 69(7): 1919-1925, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30994433

RESUMO

A Gram-stain-negative, facultative anaerobic, motile, slightly bent rod-shaped bacterium, designated strain E4404T, was isolated from coastal sediment sampled Weihai, China. According to phylogenetic analysis based on its 16S rRNA gene sequence, strain E4404T shared the highest similarity with the type strain of Vibriovariabilis (97.0 %), followed by the type strains of Vibriomaritimus (96.1 %) and Vibrioeuropaeus (96.0 %). Multilocus sequence analysis based on the 16S rRNA gene and eight housekeeping genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA) showed that strain E4404T formed a unique clade in the genus Vibrio. The average nucleotide identity and digital DNA-DNA hybridization values between strain E4404T and some other species in the genus Vibrio were 71.0-72.7 % and 20.4-22.4 %, respectively. Cells grew at 15-37 °C and pH 6.0-8.5 in the presence of 1.0-6.0 % (w/v) NaCl, with optimal growth at 33 °C and pH 7.0-7.5 in the presence of 3.0-3.5 % (w/v) NaCl. No growth was observed on thiosulfate citrate bile salts sucrose medium. The major fatty acids of strain E4404T were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The genomic DNA G+C content of strain E4404T was 46.1 mol%. Based on the phylogenetic, genotypic, phenotypic, biochemical and chemotaxonomic characteristics, strain E4404T represented a novel species of the genus Vibrio, for which the name Vibrioalbus sp. nov. is proposed. The type strain is E4404T (=MCCC 1H00197T=KCTC 52890T).


Assuntos
Sedimentos Geológicos/microbiologia , Filogenia , Água do Mar/microbiologia , Vibrio/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Genes Bacterianos , Tipagem de Sequências Multilocus , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vibrio/isolamento & purificação
11.
J Infect Public Health ; 12(5): 712-713, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30981654

RESUMO

Infections with non-O1 non-O139 Vibro cholerae strains have been reported to affect different extra intestinal sites including the urinary tract infection. We report the first case of UTI due to Vibrio albensis in an immunocompetent patient from Lebanon, where the organism was also recovered from water at his home.


Assuntos
Infecções Urinárias/diagnóstico , Infecções Urinárias/microbiologia , Vibrioses/diagnóstico , Vibrio/isolamento & purificação , Adulto , Antibacterianos/uso terapêutico , Humanos , Imunocompetência , Líbano , Masculino , Resultado do Tratamento , Infecções Urinárias/tratamento farmacológico , Vibrio/efeitos dos fármacos , Microbiologia da Água
12.
Mar Drugs ; 17(4)2019 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-30987405

RESUMO

Twenty-three bacterial strains were isolated from the secreted mucus trapping net of themarine polychaete Chaetopterus variopedatus (phylum Annelida) and twenty strains were identifiedusing 16S rRNA gene analysis. Strain CB1-14 was recognized as a new species of the genus Vibriousing the eight-gene multilocus sequence analysis (MLSA) and genome sequences of nineteen typeVibrio strains. This Vibrio sp. was cultured, and 6-epi-monanchorin (2), previously isolated from thepolychaete and two sponge species, was found in the cells and culture broth. The presence of the 6-epi-monanchorin was confirmed by its isolation followed by 1H NMR and HRESIMS analysis. Theseresults showed the microbial origin of the bicyclic guanidine alkaloid 2 in C. variopedatus.


Assuntos
Alcaloides/isolamento & purificação , Organismos Aquáticos/metabolismo , Compostos Bicíclicos Heterocíclicos com Pontes/isolamento & purificação , Guanidinas/isolamento & purificação , Poliquetos/microbiologia , Vibrio/metabolismo , Alcaloides/metabolismo , Animais , Organismos Aquáticos/genética , Técnicas de Tipagem Bacteriana/métodos , Compostos Bicíclicos Heterocíclicos com Pontes/metabolismo , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Genoma Bacteriano/genética , Guanidinas/metabolismo , Filogenia , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/isolamento & purificação , Análise de Sequência de DNA , Vibrio/genética , Vibrio/isolamento & purificação
13.
Lett Appl Microbiol ; 68(6): 485-496, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30834548

RESUMO

This study investigates the possible transmission routes of Vibrio spp. in a tropical cage-cultured marine fishes. Samplings of cultured Asian seabass, red snapper, hybrid grouper, wild fish, trash fish, fish fry, water and sediment samples were conducted from December 2016 to August 2017. All fish were dissected in situ and swabs were taken aseptically from the skin, eye, liver and kidney for bacterial isolation and identification. Bacterial isolation and identification from water, sediment and trash fish were also made. A total of 261 Vibrio spp. isolates recovered from the cultured, wild and fry fish, as well as from the sediment and water of the farm environment were analysed. Sequences of the pyrH gene were used to investigate the degree of relatedness and possible transmission routes existing between the isolated Vibrio spp. The population tree revealed the existence of selected Vibrio spp. that possibly transmitted between the newly introduced fish fry and wild fish into the cultured fish, while water also might possibly serves as natural transmission medium of certain Vibrio spp. in this fish farm. SIGNIFICANCE AND IMPACT OF THE STUDY: The source of transmission of Vibrio spp. into farmed marine fish remains unclear. This study highlights the possible transmission routes of Vibrio into cage-cultured marine fishes via newly introduced fish fry and wild fish. Understanding the routes of transmission of Vibrio spp. might help in controlling the disease in the near future.


Assuntos
Doenças dos Peixes/transmissão , Perciformes/microbiologia , Vibrioses/transmissão , Vibrioses/veterinária , Vibrio/isolamento & purificação , Animais , Aquicultura , Doenças dos Peixes/microbiologia , Pesqueiros , Peixes/microbiologia , Sedimentos Geológicos/microbiologia , Alimentos Marinhos/microbiologia , Vibrio/genética
14.
J Infect Public Health ; 12(4): 540-548, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30792073

RESUMO

BACKGROUND: Environmental diarrheagenic enteropathogens, effect of surrounding attributes and disease dynamicity remains far from being conclusively explored. Population flux, poor sanitation and hygienic practice poses potential health threat in diarrhoea endemic tropical countries like India. We aim to identify environmental attributes, seasonality of water-borne enteropathogens and health risk assessment off the river Ganges. METHODS: A yearlong sampling data generated from three sites on either sides of the River was analysed and implications have been reported. Immediately after sample collection, physico chemical and bacterial indices were measured at the sampling site and laboratory respectively, followed by further statistical analysis of the findings. RESULTS: Annual variation of physico-chemical indices viz., temperature 18°C-36°C, pH 7.49-8.67, conductivity 215-468µS/cm and turbidity 25.6-593 NTU was recorded in the riverine water samples. High temperature and turbidity were recorded in the summer and monsoon at all sites. High bacterial dispersion has been positively correlated with turbidity and temperature variation (P<0.01; P<0.1) as we report TBC 103-105CFU/ml, TCC 103-104CFU/ml and CVC 4-212CFU/ml, with higher distribution in the monsoon and reverse in the winter. This suggests that the bacterial pool proliferates at higher temperature whereas turbidity enhances their survival providing the substratum for the bacterial pool. CVC could be positively correlated with conductivity which implies that ionic content of water augments the Vibrio load. Adaptive capability of Vibrios to sustain in very low saline riverine setting seems to be assisted by turbid water coupled with nutrient rich organic matter. CONCLUSION: Our present work establishes the interplay of seasonal variants on the dynamicity of enteropathogenic bacteria in flowing aquatic ecosystem. It also categorises the existing microbial threats in the Ganga River to help monitor the conventional as well as emerging diarrhoeal pathogens to reduce diarrheal recurrences.


Assuntos
Carga Bacteriana , Diarreia/epidemiologia , Medição de Risco , Rios/microbiologia , Estações do Ano , Microbiologia da Água , Diarreia/microbiologia , Diarreia/prevenção & controle , Monitoramento Ambiental , Humanos , Higiene , Índia/epidemiologia , Saúde Pública , Saneamento , Temperatura Ambiente , Vibrio/isolamento & purificação , Doenças Transmitidas pela Água/epidemiologia , Doenças Transmitidas pela Água/microbiologia , Doenças Transmitidas pela Água/prevenção & controle
15.
Int J Syst Evol Microbiol ; 69(5): 1313-1319, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30801241

RESUMO

Strain HBUAS61001T was isolated from the pickling sauce used to make a traditional fermented food product, datoucai, in China. The strain belonged to the genus Vibrio, but was placed in a clade separate from any known Vibrio species based on the 16S rRNA gene and MLSA results. The genome consisted of two chromosomes: chromosome I was 2 901 449 bp long with a G+C content of 45.4 mol%; and chromosome II was 1 107 930 bp long with a G+C content of 45.5 mol%. The most abundant fatty acids were C16 : 0 (28.1 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c, 29.4 %) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c, 10.1 %). The isoprenoid quinones detected were Q7 and Q8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Strain HBUAS61001T could grow in the presence of up to 17 % NaCl. The calculated average nucleotide identity and in silico DNA-DNA hybridization (GGDC) values of the strain against the closest related type strains were all lower than 95 and 70 %, respectively. Putative genes in the genome associated with survival under high salinity stress were identified. Based on whole genome sequence analysis and phenotypic characteristics, strain HBUAS61001T is a new species in the genus Vibrio, and the name Vibrio zhugei (=GDMCC 1.1416T=KCTC 62784T) is proposed.


Assuntos
/microbiologia , Microbiologia de Alimentos , Filogenia , Vibrio/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vibrio/isolamento & purificação
16.
Schweiz Arch Tierheilkd ; 161(1): 43-57, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30602431

RESUMO

INTRODUCTION: Aquaculture is a rapidly growing field of food production of high economic importance. Bacterial infections are an important threat to aquaculture growth and also a common problem in ornamental fish. Some pathogenic agents and aquaculture production types are reported to be associated with increased disease. However, a detailed description of bacterial pathogens causing disease in Swiss aquaculture and ornamental pet fish is still missing. In this study we describe 1448 bacterial isolations originating from 1134 diagnostic laboratory submissions from farmed and ornamental fish in Switzerland for the period from 2000 to 2017. A strong seasonality was observed with submissions peaking in spring and summer. Bacterial isolations in fish submitted from organic farms were approximately six times more frequent than in conventional fish farms. Flavobacteriaceae, aeromonads and Yersinia ruckeri were the most common isolates from aquaculture, and motile aeromonads and Vibrio spp. were most often isolated from ornamental fish. The results of this study provide some interesting hypotheses, but further research is needed to better characterize risk factors for bacterial diseases in both aquaculture and aquarium fish in Switzerland.


Assuntos
Bactérias/isolamento & purificação , Infecções Bacterianas/veterinária , Doenças dos Peixes/microbiologia , Aeromonadaceae/classificação , Aeromonadaceae/isolamento & purificação , Fatores Etários , Animais , Bactérias/classificação , Infecções Bacterianas/microbiologia , Infecções Bacterianas/mortalidade , Doenças dos Peixes/mortalidade , Pesqueiros/classificação , Pesqueiros/normas , Peixes , Flavobacteriaceae/classificação , Flavobacteriaceae/isolamento & purificação , Agricultura Orgânica , Estudos Retrospectivos , Estações do Ano , Suíça , Vibrio/classificação , Vibrio/isolamento & purificação
17.
Mol Cell Probes ; 44: 8-13, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30610902

RESUMO

Vibrio harveyi is a pathogen that infects fish and shellfish worldwide, causing severe economic losses for the aquaculture industry. As the early diagnosis of V. harveyi infection is crucial to disease surveillance and prevention in cultured marine animals, a fast and accurate method to detect V. harveyi is required. Here, we performed recombinase polymerase amplification (RPA) using novel primers specifically designed to recognize the V. harveyi toxR gene, which encodes a transmembrane protein, and then hybridized this gene with a carboxy fluorescein (FAM)-labeled probe. The optimal conditions for the real-time RPA assay were a probe concentration of 90 nM and a 20 min incubation at 37 °C. The sensitivity of our real-time RPA assay was 50 copies of the standard plasmid, while that of real-time PCR was 500 copies. In V. harveyi-spiked Pseudosciaena crocea samples, the sensitivity of our real-time RPA was 60 CFUs per reaction, while that of PCR was 600 CFUs per reaction. SPSS probit regression analysis indicated that the limit of detection (LOD) of our RPA assay, with 95% probability, was 18 copies. The LOD was reached within 20 min and was highly reproducible across eight independent assays. Our novel RPA method successfully differentiated V. harveyi from all other tested Vibrio species, including some that were closely related. Our real-time RPA assay, in combination with a rapid DNA extraction protocol, is a fast and accurate tool for the detection of V. harveyi and for monitoring disease outbreaks. This tool will be valuable for the aquaculture industry.


Assuntos
Reação em Cadeia da Polimerase em Tempo Real/métodos , Recombinases/metabolismo , Vibrio/isolamento & purificação , Animais , Bioensaio , Primers do DNA/metabolismo , Sondas de DNA/metabolismo , Peixes/microbiologia , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
18.
Bioprocess Biosyst Eng ; 42(4): 603-610, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30617415

RESUMO

Polyhydroxybutyrates (PHB) are biodegradable polymers that are produced by various microbes, including Ralstonia, Pseudomonas, and Bacillus species. In this study, a Vibrio proteolyticus strain, which produces a high level of polyhydroxyalkanoate (PHA), was isolated from the Korean marine environment. To determine optimal growth and production conditions, environments with different salinity, carbon sources, and nitrogen sources were evaluated. We found that the use of a medium containing 2% (w/v) fructose, 0.3% (w/v) yeast extract, and 5% (w/v) sodium chloride (NaCl) in M9 minimal medium resulted in high PHA content (54.7%) and biomass (4.94 g/L) over 48 h. Addition of propionate resulted in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(HB-co-HV)) copolymer as propionate acts as a precursor for the HV unit. In these conditions, the bacteria produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) containing a 15.8% 3HV fraction with 0.3% propionate added as the substrate. To examine the possibility of using unsterilized media with high NaCl content for PHB production, V. proteolyticus was cultured in sterilized and unsterilized conditions. Our results indicated a higher growth, leading to a dominant population in unsterilized conditions and higher PHB production. This study showed the conditions for halophilic PHA producers to be later implemented at a larger scale.


Assuntos
Organismos Aquáticos , Poli-Hidroxialcanoatos/biossíntese , Água do Mar/microbiologia , Vibrio , Microbiologia da Água , Organismos Aquáticos/crescimento & desenvolvimento , Organismos Aquáticos/isolamento & purificação , Coreia (Geográfico) , Vibrio/genética , Vibrio/isolamento & purificação
19.
Int J Food Microbiol ; 288: 58-65, 2019 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-29571579

RESUMO

Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872-1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872-2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also considered simplification of the biochemical confirmation steps. In addition, because of advances in molecular based methods for identification of human pathogenic Vibrio spp. classical and real-time PCR options were also included within the scope of the validation. These considerations formed the basis of a multi-laboratory validation study with the aim of improving the precision of this ISO technical specification and providing a single ISO standard method to enable detection of these important foodborne Vibrio spp.. To achieve this aim, an international validation study involving 13 laboratories from 9 countries in Europe was conducted in 2013. The results of this validation have enabled integration of the two existing technical specifications targeting the detection of the major foodborne Vibrio spp., simplification of the suite of recommended biochemical identification tests and the introduction of molecular procedures that provide both species level identification and discrimination of putatively pathogenic strains of V. parahaemolyticus by the determination of the presence of theromostable direct and direct related haemolysins. The method performance characteristics generated in this have been included in revised international standard, ISO 21872:2017, published in July 2017.


Assuntos
Microbiologia de Alimentos/métodos , Alimentos Marinhos/microbiologia , Vibrio/fisiologia , Animais , Europa (Continente) , União Europeia , Proteínas Hemolisinas/análise , Reação em Cadeia da Polimerase em Tempo Real , Vibrio/genética , Vibrio/isolamento & purificação , Vibrio cholerae/genética , Vibrio cholerae/isolamento & purificação , Vibrio cholerae/fisiologia , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/isolamento & purificação , Vibrio parahaemolyticus/fisiologia , Vibrio vulnificus/genética , Vibrio vulnificus/isolamento & purificação , Vibrio vulnificus/fisiologia
20.
Rev Argent Microbiol ; 51(2): 170-178, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30297081

RESUMO

Steroids, including testosterone, estrone, 17ß-estradiol, estriol and 17ß-ethinyl estradiol, are harmful not only to the population dynamics of aquatic life forms but also to public health. In this study, a marine testosterone-degrading bacterium (strain N3) was isolated from Nanao Island in the South China Sea. In addition, the strain could also use 17ß-estradiol (E2), 17ß-ethinyl estradiol (EE2), estriol (E3) or cholesterol as a sole carbon source. According to the 16S rRNA gene sequence analysis, strain N3 was identified as Vibrio sp. Further characterization showed that the strain is aerobic, gram-negative, and mobile and exhibits resistance to ampicillin, carbenicillin, penicillin and spectinomycin. For enhancing its capacity of testosterone degradation, the Plackett-Burman factorial design and the central composite design were used to optimize the culture condition. Under optimal conditions, 92% of testosterone was degraded by Vibrio sp. N3 in 48h.


Assuntos
Testosterona/química , Vibrio/isolamento & purificação , Vibrio/fisiologia , Organismos Aquáticos , Biodegradação Ambiental , Vibrio/classificação
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