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1.
BMC Infect Dis ; 20(1): 618, 2020 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-32831039

RESUMO

BACKGROUND: Vibrio cholerae are oxidase-positive bacteria that are classified into various serotypes based on the O surface antigen. V. cholerae serotypes are divided into two main groups: the O1 and O139 group and the non-O1/non-O139 group. O1 and O139 V. cholerae are related to cholera infection, whereas non-O1/non-O139 V. cholerae (NOVC) can cause cholera-like diarrhea. A PubMed search revealed that only 16 cases of necrotizing fasciitis caused by NOVC have been recorded in the scientific literature to date. We report the case of a Japanese woman who developed necrotizing fasciitis caused by NOVC after traveling to Taiwan and returning to Japan. CASE PRESENTATION: A 63-year-old woman visited our hospital because she had experienced left knee pain for the past 3 days. She had a history of colon cancer (Stage IV: T3N3 M1a) and had received chemotherapy. She had visited Taiwan 5 days previously, where she had received a massage. She was diagnosed with septic shock owing to necrotizing fasciitis. She underwent fasciotomy and received intensive care. She recovered from the septic shock; however, after 3 weeks, she required an above-knee amputation for necrosis and infection. Her condition improved, and she was discharged after 22 weeks in the hospital. CONCLUSIONS: With the increase in tourism, it is important for clinicians to check patients' travel history. Clinicians should be alert to the possibility of necrotizing fasciitis in patients with risk factors. Necrotizing fasciitis caused by NOVC is severe and requires early fasciotomy and debridement followed by intensive postoperative care.


Assuntos
Fasciite Necrosante/terapia , Vibrioses/complicações , Vibrioses/terapia , Vibrio cholerae não O1/patogenicidade , Amputação , Cuidados Críticos , Diarreia/complicações , Fasciite Necrosante/diagnóstico , Feminino , Humanos , Japão , Perna (Membro)/cirurgia , Pessoa de Meia-Idade , Fatores de Risco , Choque Séptico/etiologia , Choque Séptico/microbiologia , Choque Séptico/terapia , Taiwan , Viagem , Vibrioses/diagnóstico
2.
BMC Vet Res ; 16(1): 76, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32131821

RESUMO

BACKGROUND: Vibrio alginolyticus is an important pathogen that has to be closely monitored and controlled in the mariculture industry because of its strong pathogenicity, quick onset after infection and high mortality rate in aquatic animals. Fast, simple and specific methods are needed for on-site detection to effectively control outbreaks and prevent economic losses. The detection specificity towards the pathogenic strains has to be emphasized to facilitate pointed treatment and prevention. Polymerase chain reaction (PCR)-based molecular approaches have been developed, but their application is limited due to the requirement of complicated thermal cycling machines and trained personnel. RESULTS: A fast, simple and highly specific detection method for V. alginolyticus pathogenic strains was established based on isothermal recombinase polymerase amplification (RPA) and lateral flow dipsticks (LFD). The method targeted the virulence gene toxR, which is reported to have good coverage for V. alginolyticus pathogenic strains. To ensure the specificity of the method, the primer-probe set of the RPA system was carefully designed to recognize regions in the toxR gene that diverge in different Vibrio species but are conserved in V. alginolyticus pathogenic strains. The primer-probe set was determined after a systematic screening of amplification performance, primer-dimer formation and false positive signals. The RPA-LFD method was confirmed to have high specificity for V. alginolyticus pathogenic strains without any cross reaction with other Vibrio species or other pathogenic bacteria and was able to detect as little as 1 colony forming unit (CFU) per reaction without DNA purification, or 170 fg of genomic DNA, or 6.25 × 103 CFU/25 g in spiked shrimp without any enrichment. The method finishes detection within 30 min at temperatures between 35 °C and 45 °C, and the visual signal on the dipstick can be directly read by the naked eye. In an application simulation, randomly spiked shrimp homogenate samples were 100% accurately detected. CONCLUSIONS: The RPA-LFD method developed in this study is fast, simple, highly specific and does not require complicated equipment. This method is applicable for on-site detection of V. alginolyticus pathogenic strains for the mariculture industry.


Assuntos
Técnicas de Diagnóstico Molecular/métodos , Técnicas de Amplificação de Ácido Nucleico/métodos , Vibrio alginolyticus/isolamento & purificação , Animais , Penaeidae/microbiologia , Sensibilidade e Especificidade , Vibrioses/diagnóstico , Vibrioses/veterinária , Vibrio alginolyticus/genética , Virulência/genética
3.
Chest ; 157(2): e41-e45, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32033660

RESUMO

CASE PRESENTATION: A 72-year-old man presented to our ED less than 24 hours following the acute onset of nausea, vomiting, and diarrhea. Within 12 hours of symptom onset, he noted bilateral lower extremity pain and swelling. His pain was associated with a new violaceous irregular rash on the anterior aspect of both feet and legs. There was no history of inciting trauma or recent wounds. In addition, there was no history of consumption of raw or undercooked food (including seafood) or recent change in food source. There was accompanying fever and chills for the same duration and painful swelling of his left thumb. His comorbidities included stage IIIb classical Hodgkin lymphoma diagnosed 4 months prior. His last dose of doxorubicin, bleomycin, vinblastine, and dacarbazine chemotherapy was 4 days before presentation. He had previously failed anti-CD30 monoclonal therapy resulting from attributed pancolitis.


Assuntos
Celulite (Flegmão)/diagnóstico , Gastroenterite/diagnóstico , Doença de Hodgkin/imunologia , Hospedeiro Imunocomprometido , Miosite/diagnóstico , Sepse/diagnóstico , Vibrioses/diagnóstico , Idoso , Antibacterianos/uso terapêutico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Celulite (Flegmão)/imunologia , Celulite (Flegmão)/terapia , Desbridamento , Gastroenterite/imunologia , Gastroenterite/terapia , Doença de Hodgkin/complicações , Doença de Hodgkin/tratamento farmacológico , Humanos , Dermatoses da Perna , Masculino , Miosite/imunologia , Miosite/terapia , Músculo Quadríceps/diagnóstico por imagem , Sepse/imunologia , Sepse/terapia , Infecções dos Tecidos Moles/diagnóstico , Infecções dos Tecidos Moles/imunologia , Infecções dos Tecidos Moles/terapia , Tomografia Computadorizada por Raios X , Vibrioses/imunologia , Vibrioses/terapia , Vibrio vulnificus
4.
Biosens Bioelectron ; 151: 111968, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31999578

RESUMO

In the world wide, food poisoning accidents related to Vibrio spp. are on the rise, even numbers of food poisoning by other foodborne pathogens are decreasing. Therefore, the requirement of the rapid, sensitive and convenient detection method for V. parahaemolyticus has been grown. The objective of this study is to develop a colorimetric loop-mediated isothermal amplification (LAMP) assay using a molecular beacon (HRPzyme connected with complementary oligonucleotides at the 5' and 3' ends) for the rapid, sensitive, and convenient detection of V. parahaemolyticus. The colorimetric LAMP assay optimized at 58.8°C showed a detection limit of 1 × 100 CFU/mL and was confirmed to be specific to V. parahaemolyticus. The colorimetric LAMP assay can be finished within 1 h including DNA extraction step. The method was successfully applied to flatfish samples artificially inoculated with known amount of V. parahaemolyticus, and its cut-off value for the flatfish samples was 1 × 101 CFU/g. In addition, the colorimetric LAMP assay developed in the study was found to be able to correct false-positive results, which are known to be a disadvantage of conventional LAMP assays. Therefore, these results indicated that the colorimetric LAMP method is a useful tool for the rapid, sensitive and convenient detection of V. parahaemolyticus in fishes and can also be used as a point-of-care molecular diagnostic technique since it does not require any expensive equipment such as a thermocycler and detectors.


Assuntos
Técnicas Biossensoriais , Técnicas de Diagnóstico Molecular/métodos , Técnicas de Amplificação de Ácido Nucleico/métodos , Vibrioses/diagnóstico , Vibrio parahaemolyticus/isolamento & purificação , Colorimetria , Microbiologia de Alimentos/tendências , Doenças Transmitidas por Alimentos/diagnóstico , Doenças Transmitidas por Alimentos/microbiologia , Humanos , Limite de Detecção , Alimentos Marinhos , Vibrioses/microbiologia , Vibrio parahaemolyticus/patogenicidade
5.
Diagn Microbiol Infect Dis ; 96(2): 114927, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31740171

RESUMO

Vibrio-related gastroenteritis in the United States is mostly associated with the consumption of raw or improperly cooked seafood. We describe a case of a stage IV lung adenocarcinoma patient who became ill after eating crab while visiting Upstate New York. Molecular testing and culture confirmed a coinfection with V. parahaemolyticus and a nontoxigenic strain V. cholera.


Assuntos
Cólera/complicações , Cólera/microbiologia , Coinfecção , Gastroenterite/complicações , Gastroenterite/microbiologia , Neoplasias/complicações , Vibrioses/complicações , Vibrioses/microbiologia , Adenocarcinoma de Pulmão/complicações , Adenocarcinoma de Pulmão/diagnóstico , Idoso , Cólera/diagnóstico , Coinfecção/diagnóstico , Comorbidade , Gastroenterite/diagnóstico , Humanos , Masculino , Estadiamento de Neoplasias , Neoplasias/diagnóstico , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Vibrioses/diagnóstico , Vibrio cholerae/classificação , Vibrio parahaemolyticus/classificação
6.
Artigo em Inglês | MEDLINE | ID: mdl-31799212

RESUMO

The dynamic nature of Vibrio parahaemolyticus epidemiology has presented a unique challenge for disease intervention strategies. Despite the continued rise of disease incidence and outbreaks of vibriosis, as well as the global emergence of pandemic clones and serovariants with enhanced virulence, there is a paucity of molecular methods for the serotyping of V. parahaemolyticus strains to improve disease surveillance and outbreak investigations. We describe the development of a multiplex ligation reaction based on probe melting curve analysis (MLMA) for the simultaneous identification of 11 clinically most common V. parahaemolyticus serotypes spanning a 10-year period. Through extensive sequence analyses using 418 genomes, specific primers and probes were designed for a total of 22 antigen gene targets for the O- and K- serogroups. Additionally, the toxR gene was incorporated into the assay for the confirmation of V. parahaemolyticus. All gene targets were detected by the assay and gave expected Tm values, without any cross reactions between the 11 clinically common serotypes or with 38 other serotypes. The limit of identification for all gene targets ranged from 0.1 to 1 ng/µL. The intra- and inter-assay standard deviations and the coefficients of variation were no more than 1°C and <1% respectively, indicating a highly reproducible assay. A multicenter double-blind clinical study was conducted using the traditional V. parahaemolyticus identification workflow and the MLMA assay workflow in parallel. From consecutive diarrheal stool specimens (n = 6118) collected over a year at 10 sentinel hospitals, a total of 153 V. parahaemolyticus isolates (2.5%) were identified by both workflows. A total agreement (kappa = 1.0) between the serotypes identified by the MLMA assay and conventional serological method was demonstrated. This is the first molecular assay to simultaneously identify multiple clinically important V. parahaemolyticus serotypes, which satisfies the acute need for a practical, rapid and robust identification of V. parahaemolyticus serotypes to facilitate the timely detection of vibriosis outbreaks and surveillance.


Assuntos
Tipagem Molecular , Técnicas de Amplificação de Ácido Nucleico , Vibrioses/diagnóstico , Vibrioses/microbiologia , Vibrio parahaemolyticus/classificação , Vibrio parahaemolyticus/genética , Humanos , Tipagem Molecular/métodos , Reprodutibilidade dos Testes , Sorogrupo , Sorotipagem/métodos
8.
BMJ Case Rep ; 12(10)2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31666256

RESUMO

A 63-year-old woman with a prior history of chronic lower extremity lymphedema came to the hospital for evaluation of new-onset left leg pain and swelling associated with haemorrhagic blisters and foul-smelling discharge. Relevant history included a recent trip to a Hudson River Valley beach in New York 1 week prior to hospitalisation. Laboratory evaluation revealed significant leukocytosis and lactic acidosis. She was found to have sepsis and bacteremia secondary to Vibrio cholerae (serotype non-O1, non-O139). During a prolonged intensive care unit course requiring intravenous pressor support and broad-spectrum antibiotics, she underwent debridement of her left foot eschar along with skin grafting. Once clinically stable and improved, she was discharged to a subacute rehabilitation centre with close follow-up for wound care. V. cholerae infection is rare and often benign but can be transmitted from contaminated seawater to individuals with underlying chronic illness and cause severe symptoms, including sepsis.


Assuntos
Sepse/microbiologia , Dermatopatias Infecciosas/cirurgia , Vibrioses/diagnóstico , Vibrio cholerae/isolamento & purificação , Acidose Láctica/diagnóstico , Antibacterianos/administração & dosagem , Antibacterianos/uso terapêutico , Bacteriemia/microbiologia , Vesícula/diagnóstico , Vesícula/etiologia , Desbridamento/métodos , Diagnóstico Diferencial , Feminino , Pé/microbiologia , Pé/patologia , Hemorragia/etiologia , Humanos , Unidades de Terapia Intensiva , Leucocitose/diagnóstico , Pessoa de Meia-Idade , Sepse/tratamento farmacológico , Dermatopatias Infecciosas/microbiologia , Transplante de Pele/métodos , Resultado do Tratamento , Vasoconstritores/administração & dosagem , Vasoconstritores/uso terapêutico , Vibrioses/epidemiologia
9.
Int J Food Microbiol ; 309: 108332, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31494483

RESUMO

Vibrio parahaemolyticus is a major food-borne pathogen. V. parahaemolyticus infections are associated with various serotypes; to date, 71 K-serogroups of V. parahaemolyticus have been determined based on capsular polysaccharide (CPS) diversity. In this study, the capsular polysaccharide gene clusters (CPSgcs) of 55 K-serogroups were identified by whole-genome sequencing and analysis. These CPSgcs exhibit a high level of genetic diversity. A microsphere-based suspension array (MSA) was established for the detection and identification of 55 V. parahaemolyticus K-serogroups based on CPSgc-specific genes. To evaluate our array, a double-blind test with 120 clinical isolates was carried out. In addition, an in silico K-serotyping system was established based on V. parahaemolyticus CPSgc-specific genes. This system was then used to examine 845 publicly available V. parahaemolyticus genomes; the results demonstrated that 813 isolates belong to one of 43 K-serogroups. Taken together, these results demonstrate that the molecular system developed in this study is suitable for rapid serotyping of V. parahaemolyticus isolates from environmental and clinical samples. In addition, the system could be applied to epidemiological investigations of this important food-borne pathogen.


Assuntos
Cápsulas Bacterianas/genética , Doenças Transmitidas por Alimentos/diagnóstico , Polissacarídeos Bacterianos/genética , Sorotipagem/métodos , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/imunologia , Cápsulas Bacterianas/imunologia , Método Duplo-Cego , Doenças Transmitidas por Alimentos/microbiologia , Humanos , Família Multigênica/genética , Polissacarídeos Bacterianos/imunologia , Sorogrupo , Vibrioses/diagnóstico , Vibrio parahaemolyticus/classificação
10.
J Fish Dis ; 42(11): 1523-1529, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31448425

RESUMO

As the major opportunistic pathogen to both marine animals and humans, Vibrio alginolyticus (V. alginolyticus) has caused heavy economic losses to mariculture. ssDNA aptamer VA2 targeting live V. alginolyticus was generated by systematic evolution of ligands by exponential enrichment (SELEX) technology in our previous study. In this study, we first developed aptamer (VA2)-based enzyme-linked apta-sorbent assay (VA2-ELASA) for rapid detection of mariculture pathogen V. alginolyticus. The VA2-ELASA could achieve the rapid detection for V. alginolyticus infection with high specificity and sensitivity. The VA2-ELASA could specifically identify V. alginolyticus, but not other non-target bacterial strains. VA2-ELASA could detect V. alginolyticus at the concentration of 5 × 104 /ml, the incubation time short to 1 min and the incubation temperature as high as 45°C, which proved sensitivity and stability of the novel VA2-ELASA in this study. It took less than one hour to accomplish the detection process by VA2-ELASA. The characteristics of specificity, sensitivity and easy operation make VA2-ELASA a novel useful technology for the rapid diagnosis of pathogen V. alginolyticus in mariculture.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Bacteriológicas/veterinária , Doenças dos Peixes/diagnóstico , Peixes , Vibrioses/veterinária , Vibrio alginolyticus/isolamento & purificação , Animais , Doenças dos Peixes/microbiologia , Vibrioses/diagnóstico , Vibrioses/microbiologia
11.
Food Microbiol ; 84: 103233, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31421792

RESUMO

Globally, V. parahaemolyticus infection is a leading cause of bacterial diarrheal diseases. Pathogenic V. parahaemolyticus strains that produce hemolysins are responsible for these diseases. The composition of pathogenic and non-pathogenic V. parahaemolyticus and the change of the bacterial composition before and after traditional selective enrichment in a single sample associated with disease outbreak remain unclear. We investigated an outbreak by using next generation sequencing and digital PCR to address those questions. NGS showed that the V. parahaemolyticus caused the outbreak belonged to s single clone. In contrast, among the seven non-pathogenic V. parahaemolyticus isolated from the suspected food sample, 4 serotypes and 6 PFGE patterns were identified. And nearly 70,000 SNPs were identified among the non-pathogenic strains. This result confirmed that the outbreak was caused by V. parahaemolyticus. Furthermore, NGS results clearly showed the diversity of non-pathogenic V. parahaemolyticus in a single contaminated food sample. The ratios of non-pathogenic and pathogenic V. parahaemolyticus were 31.41 and 620.11 in the original and enriched food samples respectively showed by digital PCR. Meta-genomic data indicated the top 3 species were Weissella cibaria, Weissella confusa, and Enterobacter cloacae in the original food sample, and Vibrio sp Ex25, Vibrio sp 712i, and V. parahaemolyticus in the enriched sample. Therefore, the combing of NGS and digital PCR results showed that traditional Vibrio selective enrichment media could facilitate the growth of Vibrios, however, it provided no advantages to pathogenic V. parahaemolyticus. Hence, our results indicated that the traditional culture methods alone may lead to wrong conclusions and so improvements in culture methods are needed.


Assuntos
Diarreia/microbiologia , Surtos de Doenças , Doenças Transmitidas por Alimentos/etiologia , Doenças Transmitidas por Alimentos/microbiologia , Vibrioses/diagnóstico , Vibrio parahaemolyticus/genética , Variação Genética , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Reação em Cadeia da Polimerase , Vibrioses/microbiologia , Vibrio parahaemolyticus/patogenicidade
12.
Epidemiol Infect ; 147: e243, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-31364581

RESUMO

Non-cholera Vibrio (NCV) species are important causes of disease. These pathogens are thermophilic and climate change could increase the risk of NCV infection. The El Niño Southern Oscillation (ENSO) is a 'natural experiment' that may presage ocean warming effects on disease incidence. In order to evaluate possible climatic contributions to observed increases in NCV infection, we obtained NCV case counts for the United States from publicly available surveillance data. Trends and impacts of large-scale oceanic phenomena, including ENSO, were evaluated using negative binomial and distributed non-linear lag models (DNLM). Associations between latitude and changing risk were evaluated with meta-regression. Trend models demonstrated expected seasonality (P < 0.001) and a 7% (6.1%-8.1%) annual increase in incidence from 1999 to 2014. DNLM demonstrated increased vibriosis risk following ENSO conditions over the subsequent 12 months (relative risk 1.940, 95% confidence interval (CI) 1.298-2.901). The 'relative-relative risk' (RRR) of annual disease incidence increased with latitude (RRR per 10° increase 1.066, 95% CI 1.027-1.107). We conclude that NCV risk in the United States is impacted by ocean warming, which is likely to intensify with climate change, increasing NCV risk in vulnerable populations.


Assuntos
Mudança Climática , Surtos de Doenças , Vibrioses/diagnóstico , Vibrioses/epidemiologia , Vibrio cholerae/isolamento & purificação , Animais , Centers for Disease Control and Prevention, U.S. , Cólera/diagnóstico , Cólera/epidemiologia , Bases de Dados Factuais , El Niño Oscilação Sul , Humanos , Incidência , Dinâmica não Linear , Estudos Retrospectivos , Risco , Medição de Risco , Estações do Ano , Estados Unidos
14.
Int Microbiol ; 22(4): 501-509, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31098824

RESUMO

Singleplex and duplex loop-mediated isothermal amplification (LAMP) assays were developed for detecting Vibrio anguillarum, a major bacterial pathogen of fish, and Vibrio alginolyticus, a pathogen of fish and humans, separately and simultaneously from contaminated seawater by targeting the groEL gene of V. anguillarum, which encodes a molecular chaperone protein, and the fklB gene of V. alginolyticus, which encodes a 22 kilodalton (kDa) peptidyl prolyl isomerase. The optimal reaction conditions to produce consistent results were 65 °C for 30 min, 63 °C for 30 min, and 63 °C for 40 min for the groEL (singleplex for V. anguillarum), fklB (singleplex for V. alginolyticus), and groEL + flkB (duplex) LAMP assays, respectively, analyzed via visual detection methods (use of calcein, and SYBR Green I) and agarose gel electrophoresis. The assays were found to be species-specific, as closely related Vibrio spp. were not detected. The limits of detection (LoDs) of the LAMP assays for DNA template from pure culture and artificially contaminated seawater were 10 and 14 fg (groEL assay; for V. anguillarum), 12.5 and 17 fg (fklB assay; for V. alginolyticus), and 50 and 70 fg (duplex assay) per reaction, respectively, which were much better than the LoDs of conventional polymerase chain reaction (PCR). Singleplex and duplex LAMP assays were found to be rapid, species-specific, and sensitive for the detection of V. anguillarum and V. alginolyticus and are applicable to laboratory and field diagnostics.


Assuntos
Proteínas de Bactérias/genética , Chaperonina 60/genética , Doenças dos Peixes/microbiologia , Técnicas de Amplificação de Ácido Nucleico/métodos , Vibrioses/microbiologia , Vibrioses/veterinária , Vibrio/isolamento & purificação , Animais , Doenças dos Peixes/diagnóstico , Humanos , Vibrio/genética , Vibrioses/diagnóstico , Vibrio alginolyticus/classificação , Vibrio alginolyticus/genética
16.
J Infect Public Health ; 12(5): 712-713, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30981654

RESUMO

Infections with non-O1 non-O139 Vibro cholerae strains have been reported to affect different extra intestinal sites including the urinary tract infection. We report the first case of UTI due to Vibrio albensis in an immunocompetent patient from Lebanon, where the organism was also recovered from water at his home.


Assuntos
Infecções Urinárias/diagnóstico , Infecções Urinárias/microbiologia , Vibrioses/diagnóstico , Vibrio/isolamento & purificação , Adulto , Antibacterianos/uso terapêutico , Humanos , Imunocompetência , Líbano , Masculino , Resultado do Tratamento , Infecções Urinárias/tratamento farmacológico , Vibrio/efeitos dos fármacos , Microbiologia da Água
17.
Dis Aquat Organ ; 132(3): 241-247, 2019 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-31019129

RESUMO

In June 2017, mass mortalities were reported at whiteleg shrimp Penaeus vannamei farms in Texas, USA. PCR testing for OIE-listed and non-listed pathogens detected the pirA and pirB toxin genes associated with acute hepatopancreatic necrosis disease (AHPND). DNA sequence analyses of cloned pirA and pirB genes showed them to be identical to those detected in other AHPND-causing Vibrio sp. Amplicons generated using PCR tests targeted to the toxR gene showed the Pir toxin genes to be associated with a V. parahaemolyticus type more similar to a genotype found in Mexico compared to that found in Asia. Histology detected masses of bacteria and hemocytic infiltrations as well as extensive necrosis and sloughing of epithelial cells in hepatopancreatic tubules pathognomonic of AHPND. The data support AHPND as the cause of the mortalities. Given that US companies produce shrimp broodstock for farms in Asia and Latin America, the further spread of AHPND in the USA needs to be prevented to avoid serious economic consequences to these industries.


Assuntos
Hepatopâncreas/patologia , Vibrioses/diagnóstico , Vibrio parahaemolyticus/isolamento & purificação , Doença Aguda , Animais , Humanos , Necrose , Penaeidae/microbiologia , Texas , Vibrio parahaemolyticus/genética
18.
Int J Infect Dis ; 81: 73-80, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30690211

RESUMO

BACKGROUND: Vibrio vulnificus, Aeromonas hydrophila, methicillin-resistant Staphylococcus aureus (MRSA), group A Streptococcus, and group B Streptococcus are commonly detected causative agents of necrotizing fasciitis (NF) in Chia-Yi Chang Gung Memorial Hospital. The aim of this study was to develop and evaluate a multiplex PCR method for the simultaneous detection of five of the most important human pathogens involved in NF by using a novel combination of species-specific genes. METHODS: The samples used were collected from 99 patients with surgically confirmed NF of the extremities who were hospitalized consecutively between June 2015 and November 2017. Two sets of blood and tissue samples were collected from all patients; one set was sent to a microbiology laboratory for bacterial identification and the other set was sent to an immunohistochemistry laboratory for PCR amplification. RESULTS: The multiplex PCR results for the blood samples showed negative findings. The multiplex PCR results for the tissue specimens showed 28 positive findings. Fourteen (87.5%) of the 16 V. vulnificus culture-positive tissue specimens, six (75%) of the eight A. hydrophila culture-positive tissue specimens, and four (100%) of the four MRSA culture-positive tissue specimens were positive by PCR. Similarly, two (100%) of the group A Streptococcus and two (100%) of the group B Streptococcus were PCR-positive. CONCLUSIONS: The accuracy rate of the multiplex PCR presenting positive results in these culture-positive tissue samples was 87.5% (28/32). This suggests that multiplex PCR of tissue specimens may be a useful and rapid diagnostic tool for the detection of these lethal microorganisms in patients with NF.


Assuntos
Primers do DNA/genética , Fasciite Necrosante/diagnóstico , Infecções por Bactérias Gram-Negativas/diagnóstico , Reação em Cadeia da Polimerase Multiplex , Infecções Estafilocócicas/diagnóstico , Infecções Estreptocócicas/diagnóstico , Vibrioses/diagnóstico , Aeromonas hydrophila/genética , Aeromonas hydrophila/isolamento & purificação , Fasciite Necrosante/complicações , Humanos , Staphylococcus aureus Resistente à Meticilina/genética , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Reação em Cadeia da Polimerase Multiplex/métodos , Sensibilidade e Especificidade , Streptococcus agalactiae/genética , Streptococcus agalactiae/isolamento & purificação , Streptococcus pyogenes/genética , Streptococcus pyogenes/isolamento & purificação , Vibrio vulnificus/genética , Vibrio vulnificus/isolamento & purificação
19.
Expert Rev Mol Diagn ; 19(3): 229-239, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30657706

RESUMO

INTRODUCTION: A number of DNA-based diagnostic tools have been developed for the detection of Vibrio parahaemolyticus in seafood. However, the loop-mediated isothermal amplification (LAMP) has distinct advantages with regards to its simplicity, speed and the ease of performing without any need for sophisticated equipment. Over the last decade, LAMP has emerged as a potential tool for the detection of V. parahaemolyticus. Area covered: The literature search was restricted to LAMP assay and its variants for the detection of V. parahaemolyticus. The focus in this review is to enlist the various techniques that have been developed using the principle of the LAMP towards improved simplicity, sensitivity and specificity of the assay. Expert commentary: LAMP assay and its variants are significantly faster and require minimum accessories compared to other DNA based molecular techniques such as PCR and their types. Despite the availability of several versions, LAMP-based diagnostics is not the first choice for the detection of V. parahaemolyticus in the seafood sector. Our recommendation would be to explore the possibilities of developing cost-effective LAMP kits and implementing these kits as point-of-care diagnostic tools for rapid and sensitive detection of pathogenic V. parahaemolyticus.


Assuntos
Técnicas de Diagnóstico Molecular/métodos , Técnicas de Amplificação de Ácido Nucleico/métodos , Vibrioses/diagnóstico , Vibrio parahaemolyticus/genética , Humanos , Técnicas de Diagnóstico Molecular/normas , Técnicas de Amplificação de Ácido Nucleico/normas , Sistemas Automatizados de Assistência Junto ao Leito , Sensibilidade e Especificidade , Vibrioses/microbiologia , Vibrio parahaemolyticus/patogenicidade
20.
BMJ Case Rep ; 12(1)2019 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-30659010

RESUMO

Vibrio vulnificus is a halophilic Vibrio found globally. They are thought to be normal microbiome in the estuaries along the coasts associated with seawater and seashells. Infection usually results from consumption of raw oysters or shellfish or exposure of broken skin or open wounds to contaminated salt or brackish water. Clinical manifestations range from gastroenteritis to skin and subcutaneous infection and primary sepsis. Pathogen has the ability to cause infections with significant mortality in high-risk populations, including patients with chronic liver disease, immunodeficiency, diabetes mellitus and iron storage disorders. There is often a lack of clinical suspicion in cases due to Vibrio vulnificus leading to delay in treatment and subsequent mortality. Herein we report a case of necrotising fasciitis in a diabetic patient with alcoholic liver disease caused by Vibrio vulnificus which ended fatally.


Assuntos
Fasciite Necrosante/microbiologia , Vibrioses/diagnóstico , Vibrio vulnificus/isolamento & purificação , Diabetes Mellitus/microbiologia , Evolução Fatal , Humanos , Hepatopatias Alcoólicas/microbiologia , Masculino , Pessoa de Meia-Idade
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