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1.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 38(10): 993-996, 2021 Oct 10.
Artigo em Chinês | MEDLINE | ID: mdl-34625940

RESUMO

OBJECTIVE: To investigate the genetic etiology, clinical diagnosis and treatment of a child with pancytopenia, failure to thrive and pulmonary infection. METHODS: Peripheral blood samples of the child and her parents were collected. Genomic DNA was extracted. Genetic variants associated with hematological diseases were detected by high-throughput sequencing. RESULTS: Three variants of TCN2 gene were found, one of which located in exon 5 upstream(c.581-8A>T), the parents has carried this variant; one in exon 6 (c.924_927del), the variant was originated from the mother; one in exon 7 (c.973C>T), the variant has ocurred de novo. The variants pathogenic analysis combined with clinical manifestation, pancytopenia, the increase in methylmalonic acid level and increased homocysteine, the child was diagnosed with transcobalaminIIdeficiency. The patient presented with respiratory infection, which was confirmed to be pneumocystosis by lung radioscopy and pathogenic high-throughput sequencing of broncho-alveolar lavage fluid. The patient presented with acute respiratory distress syndrome during the treatment with intramuscular injection of vitamin B12, and improved after anti-infection with compound sulfamethoxazole and symptomatic support treatment. CONCLUSION: We reported a case of Chinese child with TCNII deficiency due to novel gene variant, and analyzed the pathogenicity of the three variants. The treatment of TCNII deficiency with cobalamin should be individualized.


Assuntos
Erros Inatos do Metabolismo dos Aminoácidos , Transcobalaminas , Criança , Feminino , Testes Genéticos , Humanos , Doenças Raras , Transcobalaminas/genética , Vitamina B 12
2.
Mymensingh Med J ; 30(4): 913-920, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34605456

RESUMO

Peripheral neuropathy caused by diabetes mellitus (DM) and vitamin B12 deficiency may produce overlapping clinical pictures. Metformin use is a known cause of B12 deficiency in patients with type 2 DM (T2DM). This cross-sectional comparative study was conducted at two specialized endocrine outpatient clinics in Mymensingh and Cumilla cities of Bangladesh over one year from July 2019 to June 2020. Non-pregnant adults (≥18 years of age) receiving drug treatment for T2DM for at least six months were included in this study. The study subjects were divided into two groups: those with ongoing treatment with metformin and those who never received metformin in their lifetime. Out of 99 subjects evaluated, 66 (66.7%) were in the metformin group, and 33 (33.3%) were in the non-metformin group. Subjects in the metformin group had significantly lower B12 levels compared to the non-metformin group [448.5 (343.0-570.9) vs. 549.0 (487.5-847.0) pg/mL, median (IQR), p<0.001]. None of the study subjects in the non-metformin group were either borderline deficient or deficient of B12 compared to five borderline deficient and three deficient subjects in the metformin group. Among the study subjects, 88.9% had peripheral neuropathy (PN) (43.4% mild, 21.2% moderate and 24.2% severe PN); the two groups had similar frequencies of PN. Though median serum B12 levels were lower in mild [483.0 (411.2-620.0) pg/mL], moderate [492.0 (366.5-680.0) pg/mL] and severe PN [524.5 (363.5-654.2) pg/mL] groups compared to absent PN group [540.0 (340.0-685.0) pg/mL]; the difference in B12 levels across the four groups was not statistically significant. B12 levels had weak negative correlation (r = -0.061, p = 0.624) with gram-years of metformin use. Periodic screening for serum vitamin B12 levels should be done to identify metformin-induced B12 deficiency in T2DM, especially those with PN.


Assuntos
Diabetes Mellitus Tipo 2 , Metformina , Adulto , Bangladesh/epidemiologia , Estudos Transversais , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/tratamento farmacológico , Humanos , Hipoglicemiantes/uso terapêutico , Lactente , Metformina/uso terapêutico , Vitamina B 12
3.
Front Cell Infect Microbiol ; 11: 719542, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34604109

RESUMO

More and more studies have shown that the intestinal microbiota is the main factor in the pathogenesis of type 1 diabetes mellitus (T1DM). Beta cell expansion factor A (BefA) is a protein expressed by intestinal microorganisms. It has been proven to promote the proliferation of ß-cells and has broad application prospects. However, as an intestinal protein, there have not been studies and reports on its application in diabetes and its mechanism of action. In this study, a T1DM model induced by multiple low-dose STZ (MLD-STZ) injections was established, and BefA protein was administered to explore its therapeutic effect in T1DM and the potential mechanism of intestinal microbiota. BefA protein significantly reduced the blood glucose, maintained the body weight, and improved the glucose tolerance of the mice. At the same time, the BefA protein significantly increased the expression of ZO-1, Occludin, and significantly reduced the expression of TLR-4, Myd88, and p-p65/p65. BefA protein significantly reduced the relative expression of pro-inflammatory cytokines IL-1ß, IL-6 and TNF-α. In addition, our high-throughput sequencing shows for the first time that the good hypoglycemic effect of BefA protein is strongly related to the increase in the abundance of the beneficial gut bacteria Lactobacillus, Bifidobacterium and Oscillospria and the decrease in the abundance of the opportunistic pathogen Acinetobacter. Our group used animal models to verify the hypoglycemic effect of BefA protein, and first explored the potential mechanism of intestinal microbiota in BefA protein treatment.


Assuntos
Diabetes Mellitus Tipo 1 , Microbioma Gastrointestinal , Animais , Proliferação de Células , Diabetes Mellitus Tipo 1/tratamento farmacológico , Camundongos , Vitamina B 12/análogos & derivados
4.
J Agric Food Chem ; 69(36): 10722-10730, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34478287

RESUMO

The first multiple stable isotope dilution assay method was developed for the simultaneous determination of four cobalamins, namely, hydroxocobalamin (OHCbl), adenosylcobalamin (AdoCbl), methylcobalamin (MeCbl), and cyanocobalamin (CNCbl), in their native forms. The sample preparation was optimized with enzyme treatment and immunoaffinity purification. The analysis was performed by LC-MS/MS using respective 15N-labeled cobalamins as internal standards. Method validation resulted in limits of detection ranging from 0.19 to 0.58 ng/g and limits of quantification ranging from 0.68 to 1.73 ng/g. Recoveries at three levels were between 82 and 121%. Intra-day and inter-day precisions were below 6% and 11% RSD, respectively. The analysis of a reference material resulted in a variance of <1% from the certified value. The newly developed method demonstrated excellent sensitivity, recovery, accuracy, and reproducibility and was further applied to quantitate the four cobalamins in various meats.


Assuntos
Espectrometria de Massas em Tandem , Vitamina B 12 , Cromatografia Líquida , Isótopos , Carne , Reprodutibilidade dos Testes
6.
Clin Lab ; 67(9)2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34542961

RESUMO

BACKGROUND: We aimed to evaluate the use of population-based-reference-intervals by calculating the individuality index and reference change value of iron, transferrin, ferritin, folate, vitamin B12, and 25-OH vitamin D parameters. METHODS: A total of 5 venous blood samples were taken from 11 female and 10 male individuals on days 0, 7, 14, 21, and 28. All tests were performed on an autoanalyzer and CVI, CVG, II, and RCV values were calculated for both genders and the whole groups. RESULTS: CVA (%)/CVI (%)/CVG (%) for iron, 0.67/27.3/32.3 transferrin, 0.62/3.60/10.27 ferritin, 2.27/6.21/105.6 folate 4.71/10.3/28.56 vitamin B12, 6.1/5.77/34.6, 25-OH Vitamin D 3.4/8.2/54.9 respectively. RCV calculated as a 2-tailed value at level of probability of significant change set at 0.95 - 0.99; 74.9/98.7 - 10.1/13.3 - 18.3/24.1 - 31.4/41.3 - 23.4/30.8 - 24.7/32.5 and II were 0.8-0.4-0.-0.4-0.17-0.1 respectively. CONCLUSIONS: CVI and CVG values for iron, transferrin, folate, vitamin B12, and 25-OH vitamin D are in accordance with the literature. Ferritin was calculated differently from the values in the database. This difference might be due to the characteristics of selected individuals for study. Evaluating the suitability and utility of the use of RR with values found for II. For iron, the use of RR was considered appropriate when taken as II < 0.6 and II > 1.4. However, the use of RCV is more appropriate for Transferrin, Folate, Vitamin B12, and 25-OH Vitamin D.


Assuntos
Ferritinas , Vitamina B 12 , Feminino , Ácido Fólico , Humanos , Ferro , Masculino , Transferrina , Vitamina D
7.
Front Public Health ; 9: 676518, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34552900

RESUMO

Background: Reversible etiologies of cognitive impairment are common and treatable, yet the majority of mild cognitive impairment (MCI) and dementia research in Latin America has focused on irreversible, neurodegenerative etiologies. Objective: We sought to determine if thyroid dysfunction and vitamin B12 and folate deficiencies are associated with cognitive disorders among older adults with memory complaints in Lima, Peru. Methods: This was a retrospective review of patients who presented for cognitive evaluations to a multidisciplinary neurology clinic in Lima, Peru from January 2014 to February 2020. We included individuals aged ≥60 years, native Spanish-speakers, with at least a primary school educational level and a complete clinical assessment. Patients had either subjective cognitive decline (SCD), MCI, or dementia. One-way ANOVA and multiple logistic regression analyses were performed. Results: We included 720 patients (330 SCD, 154 MCI, and 236 dementia); the dementia group was significantly older [mean age SCD 69.7 ± 4.1, dementia 72.4 ± 3.7 (p = 0.000)] and had lower folate levels than SCD patients. The MCI group had higher free T3 levels compared with SCD patients. Those with lower TSH had greater dementia risk (OR = 2.91, 95%CI: 1.15-6.86) but not MCI risk in unadjusted models. B12 deficiency or borderline B12 deficiency was present in 34% of the dementia group, yet no clear correlation was seen between neuropsychological test results and B12 levels in our study. There was no association between MCI or dementia and thyroid hormone, B12 nor folate levels in adjusted models. Conclusion: Our findings do not support an association between metabolic and endocrine disorders and cognitive impairment in older Peruvians from Lima despite a high prevalence of B12 deficiency. Future work may determine if cognitive decline is associated with metabolic or endocrine changes in Latin America.


Assuntos
Disfunção Cognitiva , Deficiência de Ácido Fólico , Idoso , Disfunção Cognitiva/epidemiologia , Humanos , Peru/epidemiologia , Estudos Retrospectivos , Glândula Tireoide , Vitamina B 12
8.
BMC Cancer ; 21(1): 982, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34470601

RESUMO

BACKGROUND: B vitamins and methionine are essential substrates in the one-carbon metabolism pathway involved in DNA synthesis and methylation. They may have essential roles in cancer development. We aimed to evaluate the associations of dietary intakes of vitamin B12, vitamin B6, folate, and methionine with the risk of esophageal cancer (EC) using data from the Japan Public Health Center-based Prospective Study. METHODS: We included 87,053 Japanese individuals who completed a food frequency questionnaire and were followed up from 1995-1998 to 2013 and 2015. Hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated by Cox proportional-hazard regression across quintiles of dietary intakes of B vitamins and methionine. RESULTS: After 1,456,678 person-years of follow-up, 427 EC cases were documented. The multivariable HR (95% CI) of incident EC in the highest versus lowest quintile of dietary intake of vitamin B12 was 1.75 (1.13-2.71; p-trend=0.01). Stratification analysis based on alcohol consumption showed that higher dietary intakes of vitamin B12 and methionine were associated with an increased risk of EC among never-drinkers; HRs (95% CIs) were 2.82 (1.18-6.74; p-trend=0.009; p-interaction=0.18) and 3.45 (1.32-9.06; p-trend=0.003; p-interaction 0.02) for vitamin B12 and methionine, respectively. Meanwhile, there was no association between vitamin B12 and methionine intake with the risk of EC among drinkers. There were no associations between dietary intake of folate or vitamin B6 and the risk of EC. CONCLUSION: Dietary intake of vitamin B12 was positively associated with the risk of EC in the Japanese population.


Assuntos
Consumo de Bebidas Alcoólicas/fisiopatologia , Neoplasias Esofágicas/epidemiologia , Ácido Fólico/administração & dosagem , Metionina/administração & dosagem , Vitamina B 12/administração & dosagem , Vitamina B 6/administração & dosagem , Vitaminas/administração & dosagem , Idoso , Ingestão de Alimentos , Neoplasias Esofágicas/metabolismo , Feminino , Seguimentos , Humanos , Japão/epidemiologia , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos , Saúde Pública , Fatores de Risco
9.
BMJ Case Rep ; 14(8)2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34400432

RESUMO

Nitrous oxide (NO) is an inhalant that has become increasingly popular as a recreational drug. While it is presumed to be harmless, a number of adverse effects of NO have been described. We discuss the case of a 24-year-old man with no medical history, who initially presented to the emergency department with progressive polyneuropathy caused by vitamin B12 deficiency after NO abuse. Two days after being discharged with hydroxocobalamin supplementation, the patient returned with a severe headache, blurry vision and slurred speech. Imaging revealed cerebral venous sinus thrombosis. Hypercoagulability workup showed slightly elevated homocysteine and normalised vitamin B12 after supplementation. Genetic testing showed a heterozygous prothrombin G20210A mutation. He was treated with low-molecular-weight heparin followed by dabigatran. We hypothesise that NO use may increase the risk of developing cerebral venous thrombosis, especially in patients with multiple risk factors and elevated homocysteine levels.


Assuntos
Trombose Intracraniana , Trombose dos Seios Intracranianos , Deficiência de Vitamina B 12 , Adulto , Humanos , Masculino , Óxido Nitroso/efeitos adversos , Trombose dos Seios Intracranianos/induzido quimicamente , Trombose dos Seios Intracranianos/diagnóstico por imagem , Trombose dos Seios Intracranianos/tratamento farmacológico , Vitamina B 12 , Deficiência de Vitamina B 12/induzido quimicamente , Deficiência de Vitamina B 12/tratamento farmacológico , Adulto Jovem
10.
Medicina (Kaunas) ; 57(8)2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34441026

RESUMO

Background and objectives: This paper is a sequel to the studies that focused on the optimization of the structure of classical acrylates with vitamin B12 as a template and their impact on patients' general and local health. In this context, we aim to investigate the relationships between attitudes and behavior regarding oral health, oral health status, and quality of life related to oral health in the case of patients with conventional dentures and those with dentures improved with vitamin B12. Material and Methods: The sample size was estimated from previous studies, and 252 participants were enrolled and divided into two groups, one of which received as a treatment complete dentures with B12, and the other group was represented by complete edentulous patients treated with conventional complete dentures. The impact of oral health in general on patient satisfaction and quality of life was assessed using Oral Health Impact Profile OHIP-14 questionnaires. The descriptive statistical analysis was performed using SPSS 20.0. A value of p < 0.05 was considered statistically significant. Results: Differences registered between the two groups were statistically significant in all evaluated dimensions. The largest differences were recorded for physical, psychological, and social disability as well as for the level of disability, all in favor of denture B12 wearers. Conclusions: The materials used in the treatment of complete edentation can be a future research direction that can not only improve their mechanical strength but can contribute to maintaining the superior health of the oral mucosa and can also be a way of transporting substances necessary for the normal development of the metabolism of the whole body, such as vitamin B12 in our case.


Assuntos
Boca Edêntula , Qualidade de Vida , Prótese Total , Humanos , Saúde Bucal , Inquéritos e Questionários , Vitamina B 12
11.
Am J Case Rep ; 22: e931936, 2021 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-34376630

RESUMO

BACKGROUND Recreational use of nitrous oxide (laughing gas) is a growing phenomenon among young people due to easy accessibility and a presumed innocent effect. However, complications have been reported, especially following high and long-term use, including nerve damage, spontaneous pneumo-mediastinum, myocardial infarction, and macrocytic anemia. CASE REPORT We report a case of a 19-year-old previously healthy man with occasional recreational use of nitrous oxide of up to 10 times within recent months, who presented with severe peripheral neuropathy. Laboratory examination revealed severely elevated homocysteine values of 92 µmol/L (reference range, <10 µmol/L), strongly elevated methylmalonic acid level of >10 µmol/L (range, 0.1-0.4 µmol/L), vitamin B12 level of 234 pmol/L (range, 200-600 pmol/L), hemoglobin level of 9.3 mmol/L (range, 8.3-10.5 mmol/L), platelets of 384×109/L (range, 145-350×109/L), and leucocytes of 6.2×109/L (range, 3.5-10.0×109/L). Nitrous oxide can result in vitamin B12 inactivation and nerve damage due to lack of myelination. During hospitalization, the patient had a bilateral central pulmonary embolism, probably caused by a combination of nitrous oxide abuse and some extent of immobilization. After 6 months of nitrous oxide cessation and treatment with B vitamins, the patient experienced almost no residual symptoms, and homocysteine and methylmalonic acid levels normalized. CONCLUSIONS Our case shows that even moderate recreational use of nitrous oxide can lead to severe peripheral neuropathy as well as increase the risk of thromboembolic complications. Especially young and previously healthy individuals presenting with unexplained neuropathy or thromboembolic events should therefore be asked about possible use of nitrous oxide.


Assuntos
Doenças do Sistema Nervoso Periférico , Embolia Pulmonar , Deficiência de Vitamina B 12 , Adolescente , Adulto , Humanos , Masculino , Óxido Nitroso/efeitos adversos , Embolia Pulmonar/induzido quimicamente , Vitamina B 12 , Deficiência de Vitamina B 12/induzido quimicamente , Deficiência de Vitamina B 12/complicações , Adulto Jovem
12.
Int J Mol Sci ; 22(15)2021 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-34360738

RESUMO

Besides the well-known functions performed by vitamin B12 (CblCN) in biochemical processes of the human body, an increasing interest has been raised by the possibility of its use as a transmembrane drug carrier, capable, among others, of enhancing the accumulation of inorganic cytostatics in cancer cells. The present study was aimed at determining the possibility of the formation of CblCN conjugates with Pd(II) complexes. A key aspect was their stability, which we attempted to tune by appropriate choice of ligands. Syntheses, spectroscopic analysis of postreaction systems and kinetic investigations of conjugate formation reactions, have been complemented by DFT modelling. The obtained results showed that ligand charge, geometry and electron affinity may have a significant impact on carrier binding and release leading to the activation of the Pd(II) complex. This provides a rationale to expect that with appropriate composition of the coordination sphere, it will be possible to extend the spectrum of less toxic inorganic chemotherapeutics.


Assuntos
Complexos de Coordenação/química , Modelos Moleculares , Paládio/química , Vitamina B 12/química , Humanos , Ligantes
13.
J Trop Pediatr ; 67(3)2021 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-34363080

RESUMO

BACKGROUND: Infantile Tremor Syndrome (ITS) is a disorder of infancy, and characterized by developmental delay and/or regression, pallor, skin hyperpigmentation and hypopigmented hair. It is commonly seen in infants in whom exclusive breastfeeding is given inappropriately for longer durations than recommended. ITS is predominantly reported from the Indian subcontinent and in children from a lower socioeconomic background. It is a clinical diagnosis and vitamin B12 deficiency is the most commonly accepted etiology of this entity. OBJECTIVES: The primary objectives of study were to compare the plasma and urine amino acid levels among children with ITS spectrum with those of healthy children. The secondary objectives were to compare the plasma and urine amino acid levels among children with ITS and Pre-ITS. STUDY DESIGN: This cross-sectional, observational study was carried out at a tertiary care hospital in North India. PARTICIPANTS: A total of 50 children aged < 36 months with ITS/Pre-ITS were enrolled. Children with Pre-ITS and ITS were compared with healthy age-matched study subjects. RESULTS: Thirty-nine (78%) cases and twelve (24%) healthy children had low serum vitamin B12 levels. Folate levels were normal in all the controls, while only one case had folate deficiency. There were significant differences (p < 0.05) in the values of 32 amino acids in plasma. Among 44 urinary amino acids, levels of 30 amino acids were significantly different in the cases compared with the controls (p < 0.05). CONCLUSIONS: Several changes in amino acids in the children suffering from ITS were observed. These changes may be a reflection of the metabolic derangements in ITS.


Assuntos
Tremor , Deficiência de Vitamina B 12 , Aminoácidos , Criança , Estudos Transversais , Feminino , Ácido Fólico , Humanos , Lactente , Vitamina B 12
14.
Inorg Chem ; 60(17): 12681-12684, 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34382784

RESUMO

Aquacobalamin binds hydrogen peroxide reversibly to form a cobalt(III) hydroperoxo adduct with a 0.25 mM dissociation constant, as evidenced by UV-vis absorption spectroscopy and corroborated by NMR, Raman spectroscopy, stopped-flow UV-vis measurements, and density functional theory calculations.


Assuntos
Peróxido de Hidrogênio/química , Vitamina B 12/análogos & derivados , Cobalto/química , Teoria da Densidade Funcional , Espectroscopia de Ressonância Magnética , Modelos Químicos , Espectrofotometria Ultravioleta , Análise Espectral Raman , Vitamina B 12/química
15.
Nutrients ; 13(8)2021 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-34444770

RESUMO

Testing and prescribing vitamin B12 (also known as cobalamin) is increasing in Switzerland but substantial variation among general practitioners (GPs) with respect to testing has been noted. In this study, we aimed at exploring GPs' mindsets regarding vitamin B12 testing and prescribing. A cross-sectional study was conducted using an online survey distributed by e-mail to Swiss GPs. The questionnaire explored mindsets related to testing and prescribing vitamin B12 in specific clinical situations, as well as testing and prescribing strategies. The questionnaire was sent to 876 GPs and 390 GPs responded (44.5%). The most controversial domains for testing and prescribing vitamin B12 were idiopathic fatigue (57.4% and 43.4% of GPs agreed, respectively) and depressive symptoms (53.0% and 35.4% of GPs agreed, respectively). There was substantial variation among GPs with regard to testing strategies (89.5% of GPS used a serum cobalamin test, 71.3% of GPS used holotranscobalamin, and 27.6% of GPs used homocysteine or methylmalonic acid). Intramuscular injection was the predominantly prescribed route of application (median of 87.5% of the prescriptions). In this study, we focus on discordant mindsets that can be specifically targeted by using educational interventions, and research questions that still need answering specifically about the effectiveness of vitamin B12 for idiopathic fatigue.


Assuntos
Medicina Geral , Clínicos Gerais , Médicos/psicologia , Vitamina B 12/administração & dosagem , Adolescente , Adulto , Anemia/diagnóstico , Anemia/tratamento farmacológico , Atitude do Pessoal de Saúde , Técnicas de Laboratório Clínico , Estudos Transversais , Depressão/diagnóstico , Depressão/tratamento farmacológico , Fadiga/diagnóstico , Fadiga/tratamento farmacológico , Feminino , Humanos , Masculino , Polineuropatias , Padrões de Prática Médica , Inquéritos e Questionários , Suíça , Vitamina B 12/sangue , Deficiência de Vitamina B 12/diagnóstico , Deficiência de Vitamina B 12/tratamento farmacológico , Adulto Jovem
16.
Nutrients ; 13(6)2021 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-34199569

RESUMO

Vitamin B12 (also known as cobalamin) is an essential water-soluble vitamin that plays a pivotal role for several physiologic functions during one's lifespan. Only certain microorganisms are able to synthetize B12, thus humans obtain cobalamin exclusively from their diet, specifically from animal-derived foods. Specific sub-group populations are at risk of vitamin B12 subclinical deficiency due to different factors including poor intake of animal source foods and age-dependent decrease in the capacity of intestinal B12 uptake. Consumption of animal products produces some negative health issues and negatively impacts sustainability while a plant-based diet increases the risk of B12 deficiency. Taking a cue from the aforementioned considerations, this narrative review aims to summarize facts about B12 deficiency and the burden of inadequate dietary intake in elderly population, as well as to discuss sustainable approaches to vitamin B12 deficiency in aging population.


Assuntos
Dieta , Deficiência de Vitamina B 12/prevenção & controle , Vitamina B 12 , Idoso , Animais , Bactérias , Biofortificação , Biomarcadores , Alimentos Fortificados , Humanos , Micronutrientes/deficiência , Deficiência de Vitamina B 12/epidemiologia
17.
Turk J Pediatr ; 63(3): 510-515, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34254498

RESUMO

BACKGROUND: Abnormal movements such as tremors and myoclonus may be observed during both treatment and deficiency of vitamin B12, particularly in infants. Infantile tremor syndrome (ITS) is defined by the tetrad of pallor, developmental delay/regression, skin pigmentation, and brown scanty scalp hair. CASE: In this report, two cases with ITS aged less than one year who had myoclonic movements during vitamin peroral B12 treatment are discussed based on hematologic, neurological, and magnetic resonance images (MRI) findings, one of whom developed a whole-body tremor and rhythmic myoclonic movements, titubation, and restlessness in the hands and feet as well as diffuse cerebral atrophy on brain MRI. CONCLUSION: The infants of mothers with nutritional vitamin B12 deficiency may develop sudden abnormal movements following peroral vitamin B12 therapy and that the differential diagnosis of these disorders is highly important for the prevention of long-term neurological sequela by treatment.


Assuntos
Mioclonia , Deficiência de Vitamina B 12 , Feminino , Humanos , Lactente , Mães , Tremor/induzido quimicamente , Tremor/diagnóstico , Vitamina B 12 , Deficiência de Vitamina B 12/induzido quimicamente , Deficiência de Vitamina B 12/complicações , Deficiência de Vitamina B 12/diagnóstico
18.
Spectrochim Acta A Mol Biomol Spectrosc ; 263: 120194, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34303220

RESUMO

A stable, water-soluble, heightened quantum yields (QYs) Au nanoclusters by the alliance between doping Ag and dual ligands (thiosalicylic acid and bovine serum albumin) (TSA/BSA-Au/AgNCs) was prepared using one-step wet chemical synthesis. The effect of different types of aromatic thiols and the molar ratio of Au-Ag on the photo-luminescence performance of AuNCs was discussed in detail. The alloy NCs is shown to be viable fluorescent method for vitamin B12 (VB12) and chlortetracycline hydrochloride (CCH) assays, and become an excellent temperature sensor in the range of 10-50 °C. The fluorescence (FL) of TSA/BSA-Au/AgNCs was quenched with the addition of VB12 or CCH coming from Förster resonance energy transfer (FRET) combined with inner filter effect (IFE). The method can detect VB12/CCH by fluorometry with a linear response in the range of 0.33-60.0/0.33-60.0 µmol·L-1 and a 71.0/64.0 nmol·L-1 detection limit (at 3σ/slope). Furthermore, the proposed method was extended to the assays of VB12 in mineral water or tablets and CCH in veterinary drug or ointment with satisfactory results.


Assuntos
Clortetraciclina , Nanopartículas Metálicas , Corantes Fluorescentes , Ouro , Ligantes , Soroalbumina Bovina , Espectrometria de Fluorescência , Vitamina B 12
19.
Braz J Med Biol Res ; 54(10): e11355, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34287582

RESUMO

The etiology of subacute combined degeneration (SCD) of the spinal cord is closely associated with vitamin B12 (VitB12) deficiency. The clinical manifestations of SCD are complex and vary substantially. Due to some SCD patients with atypical manifestations and concomitant autoimmune disorders, the probability of misdiagnosis and missed diagnosis is still relatively high in the early stage. We report the cases of two patients who were missed or misdiagnosed at another hospital because of the normal initial VitB12 level and partial overlap of clinical manifestations, finally diagnosed as SCD with atypical manifestations and concomitant autoimmune disorders, pharyngeal-cervical-brachial Guillain-Barre syndrome in Case 1 and SCD with autoimmune thyroiditis in Case 2. After undergoing corresponding treatment, death was reported in Case 1 and improvement in Case 2. Analysis of the clinical manifestations and investigation of the underlying pathogenesis in such patients could help improve the rate of early diagnosis and allow timely treatment of SCD, thereby preventing disease progression and poor clinical outcomes.


Assuntos
Doenças Autoimunes , Degeneração Combinada Subaguda , Doenças Autoimunes/complicações , Doenças Autoimunes/diagnóstico , Humanos , Imageamento por Ressonância Magnética , Medula Espinal , Degeneração Combinada Subaguda/complicações , Degeneração Combinada Subaguda/diagnóstico , Degeneração Combinada Subaguda/patologia , Vitamina B 12
20.
Can Vet J ; 62(6): 611-616, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-34219769

RESUMO

Cobalamin concentration is often assessed in clinical practice but little is known about the significance of hypercobalaminemia. The objective of this retrospective study was to identify the conditions associated with hypercobalaminemia in dogs and to investigate association with clinicopathological variables. Medical records of dogs having serum cobalamin measured between 2016 and 2018 were reviewed. One hundred sixty dogs were included and 47 (29%) showed hypercobalaminemia. Dogs with hypercobalaminemia had gastrointestinal (57%), hepatic (11%), neurological (11%), endocrine (9%), renal (4%), pancreatic (2%), and miscellaneous (6%) diseases. Overall, 11% had neoplasia. This distribution was not significantly different from that for hypocobalaminemic and normocobalaminemic dogs. There were significantly more dogs with hyperfolatemia in the hypercobalaminemia group. These results suggest that in clinical practice hypercobalaminemia is commonly identified in gastrointestinal and hepatic disease in dogs, but can also be seen with endocrine and neurological conditions. The frequency of hyperfolatemia alongside hypercobalaminemia may reflect common metabolic pathways.


Assuntos
Doenças do Cão , Deficiência de Vitamina B 12 , Animais , Doenças do Cão/epidemiologia , Cães , Estudos Retrospectivos , Reino Unido/epidemiologia , Vitamina B 12 , Deficiência de Vitamina B 12/complicações , Deficiência de Vitamina B 12/epidemiologia , Deficiência de Vitamina B 12/veterinária
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