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2.
Bone Joint J ; 103-B(7): 1206-1214, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34192938

RESUMO

AIMS: We aimed to investigate if the use of the largest possible cobalt-chromium head articulating with polyethylene acetabular inserts would increase the in vivo wear rate in total hip arthroplasty. METHODS: In a single-blinded randomized controlled trial, 96 patients (43 females), at a median age of 63 years (interquartile range (IQR) 57 to 69), were allocated to receive either the largest possible modular femoral head (36 mm to 44 mm) in the thinnest possible insert or a standard 32 mm head. All patients received a vitamin E-doped cross-linked polyethylene insert and a cobalt-chromium head. The primary outcome was proximal head penetration measured with radiostereometric analysis (RSA) at two years. Secondary outcomes were volumetric wear, periacetabular radiolucencies, and patient-reported outcomes. RESULTS: At two years, 44 patients in each group were available for RSA assessment. The median total two-year proximal head penetration was -0.02 mm (IQR -0.09 to 0.07; p = 0.548) for the largest possible head and -0.01 mm (IQR -0.07 to 0.10; p = 0.525) for 32 mm heads. Their difference was not statistically significant (p = 0.323). Neither group demonstrated a bedding-in period. The median steady-state volumetric wear rates were 6.1 mm3/year (IQR -59 to 57) and 3.5 mm3/year (-21 to 34) respectively, and did not differ between the groups (p = 0.848). There were no statistically significant differences in periacetabular radiolucencies or patient-reported outcomes. CONCLUSION: The use of the largest possible metal head did not increase vitamin E-doped cross-linked polyethylene wear compared with 32 mm heads at two years. Linear wear was negligible and volumetric wear rates were very low in both head size groups. There was a tendency towards higher values of volumetric wear in large heads that warrants longer-term evaluation before any definite conclusions about the association between head size and wear can be drawn. Cite this article: Bone Joint J 2021;103-B(7):1206-1214.


Assuntos
Artroplastia de Quadril/instrumentação , Prótese de Quadril , Polietileno/química , Vitamina E/administração & dosagem , Idoso , Ligas de Cromo , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medidas de Resultados Relatados pelo Paciente , Porosidade , Desenho de Prótese , Falha de Prótese , Análise Radioestereométrica , Método Simples-Cego , Propriedades de Superfície
3.
Am J Physiol Regul Integr Comp Physiol ; 321(1): R49-R61, 2021 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-34075811

RESUMO

Posttraumatic stress disorder (PTSD) has been associated with an increase in risk of cardiovascular disease (CVD). The goal of this study was to determine if peripheral vascular dysfunction, a precursor to CVD, was present in young adults with PTSD, and if an acute antioxidant (AO) supplementation could modify this potential PTSD-induced vascular dysfunction. Thirteen individuals with PTSD were recruited for this investigation and were compared with 35 age- and sex-matched controls (CTRL). The PTSD group participated in two visits, consuming either a placebo (PTSD-PL) or antioxidants (PTSD-AO; vitamins C and E; α-lipoic acid) before their visits, whereas the CTRL subjects only participated in one visit. Upper and lower limb vascular functions were assessed via flow-mediated dilation and passive leg movement technique. Heart rate variability was utilized to assess autonomic nervous system modulation. The PTSD-PL condition, when compared with the CTRL group, reported lower arm and leg microvascular function as well as sympathetic nervous system (SNS) predominance. After acute AO supplementation, arm, but not leg, microvascular function was improved and SNS predominance was lowered to which the prior difference between PTSD group and CTRL was no longer significant. Young individuals with PTSD demonstrated lower arm and leg microvascular function as well as greater SNS predominance when compared with age- and sex-matched controls. Furthermore, this lower vascular/autonomic function was augmented by an acute AO supplementation to the level of the healthy controls, potentially implicating oxidative stress as a contributor to this blunted vascular/autonomic function.


Assuntos
Ácido Ascórbico/farmacologia , Sistema Nervoso Autônomo/efeitos dos fármacos , Transtornos de Estresse Pós-Traumáticos/tratamento farmacológico , Ácido Tióctico/farmacologia , Vitamina E/farmacologia , Adulto , Antioxidantes/administração & dosagem , Antioxidantes/farmacologia , Ácido Ascórbico/administração & dosagem , Pressão Sanguínea , Estudos de Casos e Controles , Quimioterapia Combinada , Feminino , Humanos , Masculino , Ácido Tióctico/administração & dosagem , Vitamina E/administração & dosagem , Adulto Jovem
4.
J Wound Care ; 30(Sup6): S44-S50, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-34120463

RESUMO

OBJECTIVE: Little is known about the efficacy of products aiming to prevent radiodermatitis, which affects between 90-95% of women with breast cancer. The use of antioxidants is promising, however, there is a lack of evidenceon their effectiveness. Here, the authors present a clinical trial protocol to evaluate the effects of applying a cream containing nanoparticles with vitamin E to prevent radiodermatitis in patients with breast cancer. METHOD: The protocol recommends that 108 women with breast cancer, receiving radiotherapy, are included in this triple-blinded, randomized, controlled study at an oncology hospital. Patients will be divided in three groups of 36 individuals each: group A will receive a cream with lipid nanoparticles and vitamin E, group B will receive a cream without nanoparticles nor vitamin E, and group C will receive a cream with nanoparticles without vitamin E. The primary endpoints will evaluate the incidence, degree, and time of onset of radiodermatitis. The secondary endpoints will focus on the quality of life, symptoms, and local temperature. Patients will be assessed three times a week, from the start of their radiotherapy treatment to two weeks after the last session. This protocol was approved by the research ethics committee of the institutions involved and registered on an international trials database.


Assuntos
Neoplasias da Mama/radioterapia , Nanopartículas/administração & dosagem , Protetores contra Radiação/administração & dosagem , Radiodermatite/prevenção & controle , Vitamina E/administração & dosagem , Administração Cutânea , Administração Tópica , Protocolos Clínicos , Feminino , Humanos , Nanopartículas/uso terapêutico , Pomadas , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento , Vitamina E/uso terapêutico
5.
J Vet Sci ; 22(3): e33, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33908207

RESUMO

Very virulent infectious bursal disease virus (vvIBDV) causes high mortality in chickens but measures to reduce the mortality have not been explored. Chickens (8-9 weeks) were treated with 3 agents before and during vvIBDV inoculation. Dexamethasone treatment reduced the mortality of infected chickens (40.7% vs. 3.7%; p < 0.001), but treatment with aspirin or vitamin E plus selenium did not affect the mortality. The bursa of Fabricius appeared to have shrunk in both dead and surviving chickens (p < 0.01). The results indicate that dexamethasone can reduce mortality in vvIBDV-infected chickens and may provide therapeutic clues for saving individual birds infected by the virus.


Assuntos
Infecções por Birnaviridae/veterinária , Galinhas , Dexametasona/farmacologia , Imunossupressores/farmacologia , Doenças das Aves Domésticas/prevenção & controle , Animais , Anti-Inflamatórios , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/farmacologia , Antioxidantes/administração & dosagem , Antioxidantes/farmacologia , Aspirina/administração & dosagem , Aspirina/farmacologia , Infecções por Birnaviridae/mortalidade , Infecções por Birnaviridae/prevenção & controle , Imunossupressores/administração & dosagem , Vírus da Doença Infecciosa da Bursa/fisiologia , Doenças das Aves Domésticas/mortalidade , Selênio/administração & dosagem , Selênio/farmacologia , Vitamina E/administração & dosagem , Vitamina E/farmacologia , Vitaminas/administração & dosagem , Vitaminas/farmacologia
6.
Acta Orthop ; 92(3): 316-322, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33517823

RESUMO

Background and purpose - The use of crosslinked polyethylene in total hip arthroplasty (THA) has decreased wear remarkably. It has been suggested that the antioxidative effects of vitamin E may enhance the wear properties of polyethylene even further. This study evaluates revision rates between vitamin E-infused polyethylene liners (E1 and E-poly, ZimmerBiomet, Warsaw, IN, USA) versus moderately crosslinked polyethylene (ModXLPE) liners from the same manufacturer used in primary THA.Patients and methods - We conducted a study based on data from the Finnish Arthroplasty Register. The study group consisted of 2,723 THAs with a vitamin E-infused liner and a reference group of 2,707 THAs with a moderately crosslinked polyethylene liner. Survivorship, revision risk, and re-revision causes were compared between groups.Results - The 7-year survival of the vitamin E-infused polyethylene liner group and of the reference group with revision for any reason as the endpoint was comparable (94% [95% CI 92.9-94.9] and 93% [CI 91.9-93.9], respectively). The adjusted hazard ratio (HR) for any revision was similar between the groups (0.7 [CI 0.4-1.1]). When revision for aseptic loosening was studied as the endpoint, the survival for the study group was 99% (CI 98.6-99.4) and for the reference group 99% (CI 98.7-99.5), and the risk of revision was comparable between the study groups (HR 1.3 [CI 0.7-2.5]).Interpretation - After an observation period of 7 years vitamin E-infused liners shows results equal to results obtained with crosslinked polyethylene liners.


Assuntos
Antioxidantes/administração & dosagem , Artroplastia de Quadril/instrumentação , Prótese de Quadril , Desenho de Prótese , Falha de Prótese , Vitamina E/administração & dosagem , Idoso , Artroplastia de Quadril/efeitos adversos , Feminino , Finlândia , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Osteoartrite do Quadril/diagnóstico , Osteoartrite do Quadril/etiologia , Osteoartrite do Quadril/cirurgia , Polietileno , Modelos de Riscos Proporcionais , Sistema de Registros , Reoperação
7.
Biochem Pharmacol ; 186: 114480, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33617844

RESUMO

Oxidative microenvironment in fibrotic liver alleviates the efficacious outcome of mesenchymal stem cells (MSCs)-based cell therapy. Recent evidence suggests that pharmacological pretreatment is a rational approach to harness the MSCs with higher therapeutic potential. Here, we investigated whether Vitamin E pretreatment can boost the antifibrotic effects of Wharton's jelly-derived MSCs (WJMSCs). We used rat liver-derived hepatocytes injured by CCl4 treatment in co-culture system with Vitamin E pretreated-WJMSCs (Vit E-WJMSCs) to evaluate the hepatoprotective effect of Vit E-WJMSCs. After 24 h of co-culturing, we found that Vit E-WJMSCs rescued injured hepatocytes as hepatocyte injury-associated medium (AST, ALT, and ALP) and mRNA (Cyp2e1, Hif1-α, and Il-1ß) markers reduced to normal levels. Subsequently, CCl4-induced liver fibrosis rat models were employed to examine the antifibrotic potential of Vit E-WJMSCs. After 1 month of cell transplantation, it was revealed that Vit E-WJMSCs transplantation ceased fibrotic progression, as evident by improved hepatic architecture and functions, more significantly in comparison to naïve WJMSCs. In addition, Vit E-WJMSCs transplantation decreased the expressions of fibrosis-associated gene (Tgf-ß1, α-Sma, and Col1α1) markers in the liver parenchyma. Intriguingly, the results of tracing experiments discovered that more WJMSCs engrafted in the Vit E-WJMSCs treated rat livers compared to naïve WJMSCs treated livers. These findings implicate that pretreatment of WJMSCs with Vitamin E improves their tolerance to hostile niche of fibrotic liver; thereby further enhancing their efficacy for hepatic fibrosis.


Assuntos
Tetracloreto de Carbono/toxicidade , Hepatócitos/efeitos dos fármacos , Cirrose Hepática/terapia , Transplante de Células-Tronco Mesenquimais/métodos , Vitamina E/administração & dosagem , Geleia de Wharton/efeitos dos fármacos , Animais , Células Cultivadas , Técnicas de Cocultura , Hepatócitos/patologia , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/patologia , Masculino , Células-Tronco Mesenquimais/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Geleia de Wharton/citologia , Geleia de Wharton/transplante
8.
Nutrients ; 13(2)2021 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-33573042

RESUMO

BACKGROUND: The study aimed to evaluate the effects of a 3-week ω-3 PUFA supplementation on serum adipocytokines (i.e., adiponectin, leptin), neuregulin-4 (NRG4) and erythrocyte omega-3 (ω-3) fatty acid content, as well as the blood antioxidant defense capacity in non-elite endurance runners. METHODS: Twenty-four runners were randomized into two groups: the supplemented group, who received omega free fatty acids extract containing 142 mg of EPA, 267 mg of DHA, 12 mg of vitamin E and 5 µg of vitamin D, each administrated at a dose of six capsules twice a day for three weeks, or the placebo group. Venous blood samples were withdrawn at the start and at the end of the study protocols to estimate serum biochemical variables. RESULTS: A significantly higher ω-3 index and lower AA/EPA ratio was observed after ω-3 PUFA compared to pre-supplementation levels (p < 0.001 and p < 0.001, respectively). An increase in baseline adiponectin and NRG4 levels, as well as a decrease of leptin concentration and lipid profile improvement, were observed in subjects after a ω-3 PUFA diet. The increased ω-3 index had a significant effect on TNFα levels and a serum marker of antioxidant defense. CONCLUSIONS: The ω-3 PUFA extract with added vitamin E and D supplementation may have a positive effect on the function of the adipocyte tissue, as well as the ability to prevent cardiovascular complications in athletes.


Assuntos
Suplementos Nutricionais , Ácidos Graxos Ômega-3/administração & dosagem , Mediadores da Inflamação/sangue , Lipídeos/sangue , Corrida/fisiologia , Adipocinas/sangue , Adulto , Biomarcadores/sangue , Doenças Cardiovasculares/prevenção & controle , Eritrócitos/metabolismo , Humanos , Masculino , Neurregulinas/sangue , Estresse Oxidativo/efeitos dos fármacos , Vitamina D/administração & dosagem , Vitamina E/administração & dosagem
9.
Neurology ; 96(6): e895-e903, 2021 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-33408141

RESUMO

OBJECTIVE: To determine whether high baseline dietary antioxidants and total nonenzymatic antioxidant capacity (NEAC) is associated with a lower risk of Parkinson disease (PD) in men and women, we prospectively studied 43,865 men and women from a large Swedish cohort. METHODS: In the Swedish National March Cohort, 43,865 men and women aged 18-94 years were followed through record linkages to National Health Registries from 1997 until 2016. Baseline dietary vitamin E, vitamin C, and beta-carotene intake, as well as NEAC, were assessed by a validated food frequency questionnaire collected at baseline. All exposure variables were adjusted for energy intake and categorized into tertiles. Multivariable Cox proportional hazard regression models were fitted to estimate hazard ratios (HRs) with 95% confidence intervals (CIs) for PD. RESULTS: After a mean follow-up time of 17.6 years, we detected 465 incidence cases of PD. In the multivariable adjusted model, dietary vitamin E (HR 0.68, 95% CI 0.52-0.90; p for trend 0.005) and vitamin C (HR 0.68, 95% CI 0.52-0.89; p for trend 0.004) were inversely associated with the risk of PD when comparing participants in the highest vs the lowest tertiles of exposure. No association was found with estimated intake of dietary beta-carotene or NEAC. CONCLUSION: Our findings suggest that dietary vitamin E and C intake might be inversely associated with the risk of PD. No association was found with dietary beta-carotene or NEAC. CLASSIFICATION OF EVIDENCE: This study provides Class III evidence that dietary vitamin E and C intake are inversely associated with the risk of PD.


Assuntos
Antioxidantes/administração & dosagem , Ácido Ascórbico/administração & dosagem , Alimentos/estatística & dados numéricos , Doença de Parkinson/epidemiologia , Sistema de Registros , Vitamina E/administração & dosagem , beta Caroteno/administração & dosagem , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Seguimentos , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Suécia/epidemiologia , Adulto Jovem
10.
ACS Appl Mater Interfaces ; 13(2): 2165-2178, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33400482

RESUMO

Oxidative damage to cells from metabolites at a wound site is one of the trickiest factors inhibiting tissue regeneration, especially with bulk damage. In addition, an excessive inflammatory reaction by the body at the wound site can make it even worse. How to scavenge the reactive oxygen species (ROS) produced from metabolism and inflammatory reactions has become a critical issue in tissue engineering. Here, we utilize the natural bioactive small molecules l-arginine and l-phenylalanine and the growth factor inositol to synthesize a branched poly(ester amide) (BPEA) to fabricate BPEA nanocapsules for vitamin E delivery at wound sites. BPEA nanocapsules loaded with vitamin E (BPEA@VE NCs) could protect cells from both extracellular and intracellular damage by scavenging ROS. Simultaneously, the inflammatory reaction could also be downregulated, benefiting from the introduction of l-arginine. Furthermore, the biodegradation products of BPEA are natural metabolites of the body, such as amino acids and growth factors, guaranteeing the biocompatibility of the BPEA@VE NCs. The protective ability of the BPEA@VE NCs was also investigated in vivo for accelerated wound healing. All the results indicate that the BPEA@VE NCs have promising potential for the modulation of the local microenvironment in tissue engineering for excellent antioxidative and anti-inflammatory properties.


Assuntos
Aminoácidos/administração & dosagem , Antioxidantes/administração & dosagem , Inositol/administração & dosagem , Nanocápsulas/química , Vitamina E/administração & dosagem , Cicatrização/efeitos dos fármacos , Aminoácidos/farmacologia , Aminoácidos/uso terapêutico , Animais , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Arginina/administração & dosagem , Arginina/farmacologia , Arginina/uso terapêutico , Células Endoteliais da Veia Umbilical Humana , Humanos , Inositol/farmacologia , Inositol/uso terapêutico , Peptídeos e Proteínas de Sinalização Intercelular/administração & dosagem , Peptídeos e Proteínas de Sinalização Intercelular/farmacologia , Peptídeos e Proteínas de Sinalização Intercelular/uso terapêutico , Masculino , Camundongos , Células NIH 3T3 , Fenilalanina/administração & dosagem , Fenilalanina/farmacologia , Fenilalanina/uso terapêutico , Poliésteres/química , Células RAW 264.7 , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo , Engenharia Tecidual , Vitamina E/farmacologia , Vitamina E/uso terapêutico
11.
Trop Anim Health Prod ; 53(1): 86, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33411090

RESUMO

This study determined the effects of antioxidant supplementation and storage time at cool temperatures on the characteristics of epididymal camel spermatozoa. Camel testes were collected at the abattoir after animal slaughtering and kept at 4 °C during transportation and until processing (max 6 h). Spermatozoa were retrieved and diluted with SHOTOR extender, split in aliquots, supplemented with the following antioxidants: 200 µm/mL vitamin E, 1.0 g/L vitamin C, 1 µg/mL selenium nanoparticles, 50 µg/mL zinc nanoparticles, 2 µg/mL sodium selenite, and 100 µg/mL zinc sulfate, and stored at 4 °C for 2, 48, 96, and 144 h. The storage time significantly affected (P < 0.05) the sperms' motility and livability, the sperms' membrane integrity, and the percentages of cytoplasmic droplets as well as the percentage of morphologically normal spermatozoa. Epididymal sperm characteristics (progressive motility, livability, membrane integrity, and abnormalities) were significantly improved (P < 0.05) when the spermatozoa were diluted with antioxidants as compared with the control group, and the best additives were identified as nano-selenium, sodium selenite, nano-zinc, and zinc sulfate. In conclusion, adding nano-sized minerals or inorganic trace elements and vitamins maintained the progressive motility, livability, and membrane integrity, and decreased abnormalities and cytoplasmic droplet percentages of epididymal camel spermatozoa stored at 4 °C up to 144 h.


Assuntos
Antioxidantes/administração & dosagem , Camelus/fisiologia , Preservação do Sêmen/veterinária , Espermatozoides/efeitos dos fármacos , Animais , Antioxidantes/farmacologia , Ácido Ascórbico/administração & dosagem , Ácido Ascórbico/farmacologia , Masculino , Nanopartículas Metálicas , Distribuição Aleatória , Selênio/administração & dosagem , Selênio/farmacologia , Vitamina E/administração & dosagem , Vitamina E/farmacologia , Zinco/administração & dosagem , Zinco/farmacologia
12.
J Oleo Sci ; 70(1): 103-112, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33431764

RESUMO

Recent research has identified minor homologs of vitamin E with one or two double bonds in the side-chain, namely tocomonoenol (T1) and tocodienol (T2), in natural products. We first explored the effectiveness of partial hydrogenation for generating minor tocochromanols from tocotrienol (T3). During hydrogenation with pure α-T3 as a substrate, the side-chain was partially saturated in a time-dependent manner, and a large amount of α-T1 and α-T2 was obtained. To investigate the beneficial effects of the hydrogenated product, we fed diabetic obese KK-A y mice with a hydrogenated T3 mixture (HT3). Feeding HT3 revealed tissue-specific accumulation of tocochromanols, ameliorated hyperglycemia and improved ratio of high-density lipoprotein cholesterol to total cholesterol in serum, with invariant body weight and fat mass. Hence, we propose that hydrogenation is a useful method for generating T1 and T2 homologs, which can be applied to explore the structure-related function of tocochromanols.


Assuntos
Diabetes Mellitus/metabolismo , Glucose/metabolismo , Metabolismo dos Lipídeos/efeitos dos fármacos , Obesidade/metabolismo , Tocotrienóis/administração & dosagem , Vitamina E/administração & dosagem , Animais , HDL-Colesterol/sangue , Diabetes Mellitus/tratamento farmacológico , Hidrogenação , Hiperglicemia/tratamento farmacológico , Hiperglicemia/metabolismo , Masculino , Camundongos Endogâmicos , Obesidade/tratamento farmacológico , Relação Estrutura-Atividade , Fatores de Tempo , Tocotrienóis/química , Tocotrienóis/farmacologia , Vitamina E/química , Vitamina E/farmacologia
13.
J Nucl Med ; 62(4): 584-590, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-32826318

RESUMO

With the successful development and increased use of targeted radionuclide therapy for treating cancer comes the increased risk of radiation injury to bone marrow-both direct suppression and stochastic effects, leading to neoplasia. Herein, we report a novel radioprotector drug, a liposomal formulation of γ-tocotrienol (GT3), or GT3-Nano for short, to mitigate bone marrow radiation damage during targeted radionuclide therapy. Methods: GT3 was loaded into liposomes using passive loading. 64Cu-GT3-Nano and 3H-GT3-Nano were synthesized to study the in vivo biodistribution profile of the liposome and GT3 individually. The radioprotection efficacy of GT3-Nano was assessed after acute 137Cs whole-body irradiation at a sublethal (4 Gy), a lethal (9 Gy), or a single high-dose administration of 153Sm-ethylenediamine-N,N,N',N'-tetrakis(methylene phosphonic acid) (EDTMP). Flow cytometry and fluorescence microscopy were used to analyze hematopoietic cell population dynamics and the cellular site of GT3-Nano localization in the spleen and bone marrow, respectively. Results: Bone marrow uptake and retention (percentage injected dose per gram of tissue) at 24 h was 6.98 ± 2.34 for 64Cu-GT3-Nano and 7.44 ± 2.52 for 3H-GT3-Nano. GT3-Nano administered 24 h before or after 4 Gy of total-body irradiation (TBI) promoted rapid and complete hematopoietic recovery, whereas recovery of controls stalled at 60%. GT3-Nano demonstrated dose-dependent radioprotection, achieving 90% survival at 50 mg/kg against lethal 9-Gy TBI. Flow cytometry of the bone marrow indicated that progenitor bone marrow cells MPP2 and CMP were upregulated in GT3-Nano-treated mice. Immunohistochemistry showed that GT3-Nano accumulates in CD105-positive sinusoid epithelial cells. Conclusion: GT3-Nano is highly effective in mitigating the marrow-suppressive effects of sublethal and lethal TBI in mice. GT3-Nano can facilitate rapid recovery of hematopoietic components in mice treated with the endoradiotherapeutic agent 153Sm-EDTMP.


Assuntos
Cromanos/administração & dosagem , Cromanos/farmacologia , Hematopoese/efeitos dos fármacos , Hematopoese/efeitos da radiação , Protetores contra Radiação/administração & dosagem , Protetores contra Radiação/farmacologia , Radioterapia/efeitos adversos , Vitamina E/análogos & derivados , Animais , Cromanos/farmacocinética , Lipossomos , Camundongos , Protetores contra Radiação/farmacocinética , Distribuição Tecidual , Vitamina E/administração & dosagem , Vitamina E/farmacocinética , Vitamina E/farmacologia
14.
Ann Hematol ; 100(3): 635-644, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33216196

RESUMO

Oxidative stress is a major mechanism contributing to the progression of ß-thalassemia. To assess the effect of vitamin E and N-acetyl cysteine (NAC) as antioxidant agents on total oxidative stress (TOS) status and total antioxidant capacity (TAC) in patients with transfusion-dependent ß-thalassemia (TDT). In this open-label randomized controlled trial, from May to August 2019, 78 eligible patients with TDT over the age of 18 were enrolled. All patients were registered at the Thalassemia Clinic of Shiraz University of Medical Sciences in Southern Iran. Patients were randomly allocated to the NAC group (10 mg/kg/day, orally), vitamin E group (10 U/kg/day, orally), and control group. The duration of the study was 3 months. The mean age of the participants was 28.5 ± 5.1 (range: 18-41) years. At the end of the study, TOS significantly decreased only in the vitamin E group (mean difference (MD), 95% confidence interval (CI): 0.27 (0.03-0.50), P = 0.026). TAC significantly decreased in both supplemented groups at the 3rd month of treatment (NAC group: MD (95% CI): 0.11 (0.04-0.18), P = 0.002 and vitamin E group: 0.09 (0.01-0.16), P = 0.022 respectively). Hemoglobin did not significantly change at the end of the study in each group (P > 0.05). Mild transient adverse events occurred in 4 patients of the NAC group and 5 patients of the vitamin E group with no need to discontinue the treatment. Vitamin E can be a safe and effective supplement in improving oxidative stress in patients with TDT. Moreover, it seems that a longer duration of using antioxidant supplements needs to make clinical hematologic improvement in TDT patients.


Assuntos
Acetilcisteína/administração & dosagem , Estresse Oxidativo/efeitos dos fármacos , Vitamina E/administração & dosagem , Talassemia beta/tratamento farmacológico , Acetilcisteína/farmacologia , Adolescente , Adulto , Antioxidantes/administração & dosagem , Antioxidantes/análise , Antioxidantes/metabolismo , Transfusão de Sangue , Suplementos Nutricionais , Feminino , Humanos , Irã (Geográfico) , Masculino , Oxidantes/sangue , Oxirredução/efeitos dos fármacos , Fatores de Tempo , Vitamina E/farmacologia , Adulto Jovem , Talassemia beta/sangue , Talassemia beta/terapia
15.
Carbohydr Polym ; 251: 116988, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33142560

RESUMO

Potential benefit of microencapsulation is its ability to deliver and protect incorporated ingredients such as vitamin E. Microcapsule wall properties can be changed by adding of coss-linking agents that are usually considered toxic for application. The microcapsules were prepared by a spray-drying technique using coacervation method, by depositing the coacervate formed in the mixture of chitosan and sodium lauryl ether sulfate to the oil/water interface. All obtained microcapsules suspensions had slightly lower mean diameter compared to the starting emulsion (6.85 ± 0.213 µm), which shows their good stability during the drying process. The choice and absence of cross-linking agents had influence on kinetics of vitamin E release. Encapsulation efficiency of microcapsules without cross-linking agent was 73.17 ± 0.64 %. This study avoided the use of aldehydes as cross-linking agents and found that chitosan/SLES complex can be used as wall material for the microencapsulation of hydrophobic active molecules in cosmetic industry.


Assuntos
Composição de Medicamentos/métodos , Vitamina E/administração & dosagem , Cápsulas/química , Quitosana/química , Cosméticos/administração & dosagem , Cosméticos/farmacocinética , Reagentes para Ligações Cruzadas , Preparações de Ação Retardada/administração & dosagem , Preparações de Ação Retardada/química , Preparações de Ação Retardada/farmacocinética , Fármacos Dermatológicos/administração & dosagem , Fármacos Dermatológicos/farmacocinética , Dessecação , Emulsões , Humanos , Interações Hidrofóbicas e Hidrofílicas , Técnicas In Vitro , Cinética , Microscopia Eletrônica de Varredura , Tamanho da Partícula , Polietilenoglicóis/química , Vitamina E/farmacocinética
16.
Molecules ; 25(23)2020 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-33260376

RESUMO

The aim of the present study was to evaluate the effects of supplementation with a fixed combination of citicoline 500 mg, homotaurine 50 mg, and vitamin E 12 mg (CIT/HOMO/VITE) on contrast sensitivity and visual-related quality of life in patients with primary open-angle glaucoma (POAG) in mild stage. This was a multicenter, observational, cross-over, short-term, pilot study on POAG patients with stable controlled intraocular pressure (IOP). Patients were randomly assigned to Group 1 (current topical therapy for 4 months and then current topical therapy plus CIT/HOMO/VITE for 4 months) or Group 2 (CIT/HOMO/VITE in addition to current topical therapy for 4 months and then topical therapy alone for 4 months). Best-corrected visual acuity, IOP, visual field, and the Spaeth/Richman contrast sensitivity (SPARCS) test score were recorded at baseline and after 4 and 8 months. The Glaucoma Quality of Life-15 (GQL-15) questionnaire was administered at each check time. Forty-four patients were assigned to Group 1 and 65 to Group 2. Over the follow-up period, there were no significant changes in IOP or visual field findings, whereas SPARCS and GQL-15 findings significantly varied from baseline, both being improved in subjects treated with CIT/HOMO/VITE fixed combination. These results demonstrate that a daily intake of a fixed combination of citicoline, homotaurine, and vitamin E in addition to the topical medical treatment significantly increased the total score of the contrast sensitivity test and the quality of life in patients with POAG.


Assuntos
Antioxidantes/farmacologia , Citidina Difosfato Colina/farmacologia , Glaucoma de Ângulo Aberto/tratamento farmacológico , Fármacos Neuroprotetores/farmacologia , Taurina/análogos & derivados , Vitamina E/farmacologia , Administração Tópica , Idoso , Idoso de 80 Anos ou mais , Antioxidantes/administração & dosagem , Sensibilidades de Contraste/efeitos dos fármacos , Estudos Cross-Over , Citidina Difosfato Colina/administração & dosagem , Quimioterapia Combinada , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fármacos Neuroprotetores/administração & dosagem , Projetos Piloto , Qualidade de Vida , Distribuição Aleatória , Inquéritos e Questionários , Taurina/administração & dosagem , Taurina/farmacologia , Vitamina E/administração & dosagem
17.
Rev Paul Pediatr ; 39: e2019295, 2020.
Artigo em Inglês, Português | MEDLINE | ID: mdl-33331545

RESUMO

OBJECTIVE: To assess vitamin E intake and its relationship with sociodemographic variables, and to identify the main dietary sources of the nutrient in the diet of adolescents. METHODS: This is a population-based cross-sectional study that used data from 891 adolescents living in Campinas, SP, participating in ISACamp 2014/15 (Health Survey) and ISACamp-Nutri 2015/16 (Food Consumption and Nutritional Status Survey). The nutrient intake averages were estimated using the Generalized Linear Model, adjusted for the total energy of the diet. Dietary sources of vitamin E were identified from the calculation of the relative contribution. RESULTS: The average vitamin E intake was 3.2 mg for adolescents aged 10 to 13 years and 3.5 mg for those aged 14 to 19 years, results far below the recommended values of 9 and 12 mg, respectively. The prevalence of inadequacy was 92.5%. -Ten -foods/-food groups represented 85.7% of vitamin E present in the adolescents' diet; the vegetable oils group accounted for more than a quarter of the contribution (25.5%), followed by cookies (9.1%) and beans (8.9%). CONCLUSIONS: There were a low intake and a high prevalence of inadequate vitamin E intake among adolescents in Campinas, with vegetable oil as the main source. For the total number of adolescents, almost 33% of the nutrient content was derived from foods of poor nutritional quality such as cookies, packaged snacks, and margarine. The results of this study can guide public health actions that aim to improve the quality of adolescents' diets.


Assuntos
Comportamento Alimentar , Estado Nutricional , Vitamina E/análise , Adolescente , Antioxidantes/administração & dosagem , Antioxidantes/análise , Brasil , Criança , Estudos Transversais , Dieta Ocidental/efeitos adversos , Gorduras na Dieta/administração & dosagem , Feminino , Humanos , Masculino , Inquéritos Nutricionais , Necessidades Nutricionais , Vitamina E/administração & dosagem
18.
Food Funct ; 11(12): 10665-10674, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33216086

RESUMO

Vitamin E (VE) plays a crucial role in regulating the physiological functions of animals. In the present study, the mechanism by which VE regulates the activities of antioxidant enzymes through Wnt10b signaling was investigated in the muscle of zebrafish. It was found that the gene expression of Wnt10b, ß-catenin, and PPARγ was induced, while the GSK-3ß expression was inhibited by 52.34 and 101.27 mg kg-1 VE treatments. The generation of a hydroxy radical (˙OH) and superoxide anion (O2˙-) and the content of hydrogen peroxide (H2O2) were decreased by VE treatments. However, the activities of superoxide dismutase (SOD), peroxidase (POD), and glutathione peroxidase (GSH-PX) were increased by 52.34 and 101.27 mg kg-1 VE treatments. In addition, the content of saturated fatty acids (SFA) was decreased, but that of polyunsaturated fatty acids (PUFA) was increased by VE treatment. To confirm the role of Wnt10b in regulating antioxidant functions, Wnt10b RNA was interfered in zebrafish fed with different concentrations of VE diets. The results showed that the GSK-3ß gene expression was induced but the ß-catenin expression was inhibited by Wnt10b RNA interference in the muscle of zebrafish. The levels of O2˙-, H2O2, and ˙OH were enhanced, but the activities of SOD, GSH-PX, and POD were decreased by the interference of Wnt10b RNA. In all, our results indicated that VE could induce the Wnt10b/ß-catenin signaling pathway, which may further regulate the activities of antioxidant enzymes in the muscle of zebrafish.


Assuntos
Antioxidantes/farmacologia , Dieta , Músculos/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Vitamina E/administração & dosagem , Proteínas Wnt/metabolismo , Animais , Ácidos Graxos/metabolismo , Expressão Gênica , Glutationa Peroxidase/metabolismo , Glicogênio Sintase Quinase 3 beta , Peróxido de Hidrogênio/metabolismo , Proteínas Proto-Oncogênicas/genética , Transdução de Sinais , Superóxido Dismutase/metabolismo , Proteínas Wnt/genética , Peixe-Zebra , beta Catenina/genética , beta Catenina/metabolismo
19.
PLoS One ; 15(10): e0241085, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33095820

RESUMO

In recent years, the ex situ population of the endangered black-footed ferret (Mustela nigripes; ferret) has experienced a decline in normal sperm morphology (from 50% to 20%), which may be linked to inbreeding depression and/or a dietary change. We examined the effects of adding carcass and vitamin E to the diet on stress and reproductive biomarkers in male ferrets (n = 42 males including 16 juveniles and 26 adults) housed at the U.S. Fish and Wildlife National Black-footed Ferret Conservation Center (Carr, CO, USA). Fecal samples (3x/week) were collected from November and December (pre-breeding season, no diet change), February through May (breeding season, diet change) and June (post-breeding season, diet change) and analyzed for fecal glucocorticoid metabolites (FGM) via a corticosterone enzyme immunoassay (EIA). A subset of samples from adult males (n = 15) were analyzed for fecal androgen metabolites (FAM) via a testosterone EIA. We first used a linear mixed effects model to identify the important fixed effects among meat treatment, vitamin E treatment, age class (juvenile or adult), and all possible interactions on each hormone. We then examined the important factor's effects across seasons using the non-parametric Friedman test. We found that age did not influence (p = 0.33) FGMs; however there was a significant effect of meat treatment on FGM (p = 0.04) and an effect of vitamin E on FAMs (p<0.10). When fed carcass, FGMs declined (p<0.001) from pre- to the during the breeding season time period, but was similar (p>0.05) between during and post-breeding season periods. Males that were not fed carcass had higher (p<0.05) FGMs during the breeding season compared to pre- and post-breeding season and FGMs were lower (p<0.05) in the post-breeding season compared to pre-breeding season. Males fed with carcass had lower (p<0.001) FGM than males that were not fed carcass during both the pre-breeding and the breeding season but not during the post-breeding season (p>0.05). Males supplemented with vitamin E had higher (p<0.001) FAM than non-supplemented males during the breeding season only. For both groups, FAM was highest (p<0.05) during the breeding season. In conclusion, adding carcass to the diet can reduce glucocorticoid production and adding vitamin E can increase testosterone during the breeding season, which may influence reproductive success.


Assuntos
Ração Animal , Espécies em Perigo de Extinção , Furões/fisiologia , Reprodução/fisiologia , Vitamina E/administração & dosagem , Animais , Suplementos Nutricionais , Fezes/química , Comportamento Alimentar/fisiologia , Glucocorticoides/análise , Glucocorticoides/metabolismo , Masculino , Carne/efeitos adversos , Reprodução/efeitos dos fármacos , Estações do Ano , Testosterona/análise , Testosterona/metabolismo
20.
Nutrients ; 12(10)2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33053816

RESUMO

Patients with intestinal fat malabsorption and urolithiasis are particularly at risk of acquiring fat-soluble vitamin deficiencies. The aim of the study was to evaluate the vitamin status and metabolic profile before and after the supplementation of fat-soluble vitamins A, D, E and K (ADEK) in 51 patients with fat malabsorption due to different intestinal diseases both with and without urolithiasis. Anthropometric, clinical, blood and 24-h urinary parameters and dietary intake were assessed at baseline and after ADEK supplementation for two weeks. At baseline, serum aspartate aminotransferase (AST) activity was higher in stone formers (SF; n = 10) than in non-stone formers (NSF; n = 41) but decreased significantly in SF patients after supplementation. Plasma vitamin D and E concentrations increased significantly and to a similar extent in both groups during intervention. While plasma vitamin D concentrations did not differ between the groups, vitamin E concentrations were significantly lower in the SF group than the NSF group before and after ADEK supplementation. Although vitamin D concentration increased significantly in both groups, urinary calcium excretion was not affected by ADEK supplementation. The decline in plasma AST activity in patients with urolithiasis might be attributed to the supplementation of ADEK. Patients with fat malabsorption may benefit from the supplementation of fat-soluble vitamins ADEK.


Assuntos
Síndromes de Malabsorção/sangue , Urolitíase/sangue , Vitamina A/sangue , Vitamina D/sangue , Vitamina E/sangue , Vitamina K/sangue , Adulto , Idoso , Aspartato Aminotransferases/sangue , Colesterol/sangue , Suplementos Nutricionais , Feminino , Humanos , Síndromes de Malabsorção/complicações , Síndromes de Malabsorção/terapia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Triglicerídeos/sangue , Urolitíase/complicações , Urolitíase/terapia , Vitamina A/administração & dosagem , Deficiência de Vitamina A/sangue , Deficiência de Vitamina A/etiologia , Deficiência de Vitamina A/terapia , Vitamina D/administração & dosagem , Deficiência de Vitamina D/sangue , Deficiência de Vitamina D/etiologia , Deficiência de Vitamina D/terapia , Vitamina E/administração & dosagem , Deficiência de Vitamina E/sangue , Deficiência de Vitamina E/etiologia , Deficiência de Vitamina E/terapia , Vitamina K/administração & dosagem , Deficiência de Vitamina K/sangue , Deficiência de Vitamina K/etiologia , Deficiência de Vitamina K/terapia , Vitaminas/administração & dosagem , Vitaminas/sangue
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