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2.
Food Chem ; 311: 125929, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31855772

RESUMO

The effects of sugar component ratio, water fraction, and storage conditions on crystallisation and glass transition temperature (Tg) of three Chilean dried raisins were examined by using differential scanning calorimetry (DSC), polarised light microscopy and X-ray diffraction (XRD) techniques. Thompson, Flame and Golden raisins differed in fructose:glucose and glucose:water ratios, impacting on their measured Tgs (P < 0.05) and propensity of sugaring. The ratios of fructose:glucose (1:3, 1:2 and 1:1) and glucose:water (1.7, 1.9 and 2.1) also influenced the measured Tgs (P < 0.05) and sugaring of fructose-glucose-water model solutions. Measurement of Tgs in the raisins as a function of water activity (aw 0.11-0.74) showed that water acts as a strong plasticiser decreasing the Tgs (-16.4 to -61.6 °C). XRD results revealed that sugaring in Thompson raisins was delayed at low temperatures (5 & 15 °C) compared to that stored at 25 °C. The refrigeration may be a simple approach to delay the sugaring in raisins.


Assuntos
Açúcares/química , Vitis/química , Varredura Diferencial de Calorimetria , Chile , Temperatura Baixa , Cristalização , Frutas/química , Vidro/química , Temperatura de Transição , Vitrificação , Água/análise , Difração de Raios X
3.
Theriogenology ; 141: 219-227, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31084918

RESUMO

Male and female embryos are known to be different in developmental kinetics, metabolism, gene expression, and epigenetic patterns. Therefore, the objective of this study was to clarify whether the morphological criteria used to select embryos for cryopreservation lead to a deviation in the male:female ratio, and whether vitrification effects vary according to embryo sex. Initially, five sires were tested to evaluate the effect of the bull on embryo development, sex ratio, speed of development, and response to cryopreservation. Results showed that bulls affected (P < 0.05) embryo production, response to cryopreservation, and sex ratio. Then, one bull was selected, and used to produce embryos in vitro to characterize the responses of male and female embryos to vitrification. Results suggested that male and female embryos have the same morphological responses to vitrification, as no differences (P > 0.05) were observed between the two sexes in post-warming survival and re-expansion rates. However, their molecular responses as evaluated by gene expression (FOSL1, HSPB1, CASP3, CASP8, HSPA5, HSPA1A, G6PD, and PGK1) analysis indicated an effect of sex on vitrification; vitrified female embryos exhibited higher mRNA levels of HSPA1A, CASP3, and G6PD compared to their male counterparts. In conclusion, bulls affected embryo production, speed of development, sex ratio, and response to cryopreservation. Male and female embryos differed in their molecular responses to vitrification; and also, deviations in the male:female ratio when selecting embryos for cryopreservation were confirmed.


Assuntos
Blastocisto/fisiologia , Bovinos/embriologia , Criopreservação/veterinária , Técnicas de Cultura Embrionária/veterinária , Animais , Desenvolvimento Embrionário/fisiologia , Feminino , Masculino , Fatores Sexuais , Vitrificação
4.
Waste Manag ; 102: 932-938, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31855693

RESUMO

In this paper, B2O3 was used as a fluxing agent to reduce the melting temperature of municipal solid waste incineration (MSWI) fly ash and promote the glassy phase formation. Ash fusion temperature (AFT) test, atomic absorption spectroscopy (AAS), X-ray diffraction(XRD), scanning electron microscope (SEM) and thermodynamic calculation were carried out. The results showed that the flow temperature decreased from 1211 °C to 986 °C with an increase in the B2O3 content from 0 to 15 wt%. The melting slag lead to a decrease in the surface area and an increase in the mass transfer resistance of heavy metals. Thus, the agglomeration of the fly ash particles became easier with the reduced viscosity of the liquid slag. The volatilization of Pb, Cd and Zn in fly ash was inhibited. The leaching behavior of heavy metals was evaluated, the results indicated that Zn and Cu were stable whereas the Pb and Cd were soluble in glassy slag.


Assuntos
Metais Pesados , Eliminação de Resíduos , Carbono , Cinza de Carvão , Incineração , Material Particulado , Resíduos Sólidos , Vitrificação
5.
Pol J Vet Sci ; 22(4): 661-666, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31867919

RESUMO

In this study, the effects of oleic (18:1 cis-9-octadecenoic acid) and linoleic (18:2 (n-6), 9,12-octadecadienoic acid) acids added to the embryo culture media for bovine embryonic development after vitrification were investigated in cattle. Following maturation and fertilization, the oocytes were placed in Charles Rosencrans (CR1aa) culture drops containing 10, 100, 500, and 1000 µM of oleic or linoleic acids. On day 7 or 8 of the culture, the blastocysts and expanded blastocysts were vitrified and warmed to evaluate the viability and development. High doses of oleic acid (1000 µM) in the culture media increased the viability of embryos after vitrification. Similarly, linoleic acid at 1000 µM increased the viability compared to the other linoleic acid doses. It was observed that the addition of essential fatty acids improved the development of embryos. Increasing the concentration of linoleic and oleic acid concentrations in the media proportionally advanced the embryonic development and hatching capability after vitrification/ /warming. Specifically, the addition of high doses of oleic acid had dramatic effects on the embryonic development after vitrification/warming probably due to the increased lipid storage. In conclusion, the present results suggest that the ratio of unsaturated fatty acids in the culture media affects significantly the embryonic development in vitro.


Assuntos
Bovinos/embriologia , Embrião de Mamíferos/efeitos dos fármacos , Ácido Linoleico/farmacologia , Ácido Oleico/farmacologia , Preservação de Tecido/veterinária , Vitrificação , Animais , Criopreservação/veterinária , Meios de Cultura , Técnicas de Cultura Embrionária/veterinária , Desenvolvimento Embrionário
6.
Molecules ; 24(19)2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31581496

RESUMO

The first mechanical relaxation measurements (f = 400 Hz) of water confined in micro-porous silica were performed more than 40 years ago. The authors reported a so called "capillary transition" (here denoted as P3) of water in the core of the pores and a second one at a lower temperature, which they called the "adsorbate transition" (P1 in present work) related to water near the surface of the pores. The capillary transition was identified with the freezing of water in the centre of the pores. However, even 40 years later, the origin of the adsorbate transition is not yet clear. One study relates it to the liquid-to-glass transition of the supercooled water in the pores, and another study to the freezing of the proton reorientations at the lattice defects. The present work shows the data from extensive dynamic mechanical analysis (DMA) measurements (f = 0.1 Hz-70 Hz) of water confined in mesoporous silica (d = 2.5, 5 and 10 nm), which are in favour of a liquid-to-glass scenario.


Assuntos
Dióxido de Silício/química , Água/química , Congelamento , Simulação de Dinâmica Molecular , Porosidade , Termodinâmica , Vitrificação
7.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 36(5): 850-855, 2019 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-31631635

RESUMO

There is a great demand for blood and stem cells in clinic. It is difficult to achieve high throughput and to increase the cooling rate at the same time during vitrification. In this paper, a micro-droplet spray system with a container collection device was fabricated, and HepG2 cells were sprayed by this system for high-throughput vitrification. First, the container collection device and a cryo-paper were used to receive micro-droplets in the spray vitrification system. The results showed that the cell survival rate and 24h adhesion rate in container collection vitrification group were significantly higher than those in cryo-paper collection group. Second, HepG2 cells were sprayed and vitrified at increased cell density, and it was found that the results of micro-droplet spray vitrification did not change significantly. Finally, micro-droplet spray vitrification is compared with slow freezing. Cell processing capacity in the vitrification group increased, meanwhile, the cell survival rate and 24h adhesion rate in the vitrification group were significantly higher than those in slow freezing group. The results indicated that the micro-droplet spray vitrification system with container collection device designed in this paper can achieve high-throughput cell vitrification, which is of great significance for mass preservation of small cells.


Assuntos
Criopreservação , Vitrificação , Adesão Celular , Sobrevivência Celular , Células Hep G2 , Humanos
8.
Reprod Domest Anim ; 54 Suppl 3: 53-56, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31512317

RESUMO

DNA fragmentation of cumulus cells could be used as an indicator of oocyte vitrification success as an indirect indicator of the quality of the oocyte. This study was designed to compare the DNA fragmentation of post-mortem equine cumulus cells before or after vitrification in the absence of permeable cryoprotectant agents. Cumulus-oocyte complexes (COCs; n = 56) were recovered from slaughterhouse ovaries and subjected to in vitro maturation (42 hr/38.2°C/5%CO2 ) before (control group) or after a permeable cryoprotectant-free vitrification method using 1 M sucrose (vitrification group). After in vitro maturation, COCs were denuded, and cumulus cells were washed and stored at -80°C until thawing. Cumulus cell samples were processed with the chromatin dispersion test (Ovoselect, Halotech DNA, Spain). Low, high and total DNA fragmentation percentages of cumulus cells were recorded and compared between the two groups by Student's t test. Results were expressed as mean ± SEM. The vitrified group resulted in significantly higher (p < 0.05) percentages for low (16.81 ± 1.62 vs. 6.63 ± 0.77) and total (21.14 ± 1.84 vs. 12.76 ± 1.48) DNA fragmentation of cumulus cells. There were no significant differences between groups for high DNA fragmentation of cumulus cells. In conclusion, permeable cryoprotectant-free vitrification of equine oocytes increased the total DNA fragmentation rate of cumulus cells but protected them against high DNA fragmentation rates. Further studies are needed to examine the relationship between DNA fragmentation of cumulus cells and the developmental competence of equine oocytes.


Assuntos
Criopreservação/veterinária , Células do Cúmulo , Fragmentação do DNA , Oócitos , Animais , Criopreservação/métodos , Feminino , Cavalos , Vitrificação
9.
Food Chem ; 301: 125187, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31387041

RESUMO

Solid and liquid components coexist into glassy and amorphous structures of food complex matrixes. Both states admit movements, promoting physical modifications to a more thermodynamically stable system. Green and roasted coffee beans are principally characterized by a glassy structure that slowly evolves during storage. The aim of this study was to assess calorimetric and dielectric properties in combination, as a useful multi-analytical technique to improve the understanding of the motion mechanism of localized molecules. After equilibration at different water activities (aw) for the determination of sorption isotherms of green and roasted coffee, the glass transition temperature (Tg) of the samples has been measured by using differential scanning calorimetry (DSC). Increasing the aw from 0.155 to 0.512, the Tg shifted from 48.76 (±0.04) to 34.89 (±0.02) °C for green coffee and from 45.73 (±0.05) to 40.15 (±0.10) °C for the roasted one. The spectroscopic fingerprint of the matrix has been determined by dielectric measurements in terms of "gain" spectra (related to the imaginary part of permittivity). The maximum values of the determination coefficient (R2), obtained by linear correlation between spectral data and water activity or glass transition values for a specific frequency of the whole range (1.6 GHz-2.7 GHz), were 0.999 and 0.943 for green, and 0.997 R2 and 0.925 R2 for roasted coffee respectively.


Assuntos
Coffea/química , Café/química , Varredura Diferencial de Calorimetria , Temperatura de Transição , Vitrificação , Água/química
10.
J Assist Reprod Genet ; 36(9): 1823-1835, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31376104

RESUMO

PURPOSE: Vitrification is a well-accepted fertility preservation procedure for cryopreservation of oocytes and embryos but little is known regarding ovarian tissue, for which slow freezing is the current convention. The aim of the present study was to assess the efficiency of non-equilibrium vitrification compared to conventional slow freezing for ovarian cortex cryopreservation. METHODS: Using prepubertal sheep ovaries, the capacity of the tissue to sustain folliculogenesis following cryopreservation and in vitro culture was evaluated. Ovarian cortex fragments were cultured in wells for 9 days, immediately or after cryopreservation by conventional slow freezing or non-equilibrium vitrification in straws. During culture, follicular populations within cortex were evaluated by histology and immunohistochemistry for PCNA and TUNEL. Steroidogenic activity of the tissue was monitored by assay for progesterone and estradiol in spent media. RESULTS: No significant differences in follicle morphology, PCNA, or TUNEL labeling were observed between cryopreservation methods at the initiation of culture. Similar decreases in the proportion of primordial follicle population, and increases in the proportion of growing follicles, were observed following culture of fresh or cryopreserved ovarian tissue regardless of cryopreservation method. At the end of culture, PCNA and TUNEL-positive follicles were not statistically altered by slow freezing or vitrification in comparison to fresh cultured fragments. CONCLUSIONS: Overall, for both cryopreservation methods, the cryopreserved tissue showed equal capacity to fresh tissue for supporting basal folliculogenesis in vitro. Taken together, these data confirm that both non-equilibrium vitrification and slow-freezing methods are both efficient for the cryopreservation of sheep ovarian cortex fragments.


Assuntos
Criopreservação/métodos , Folículo Ovariano , Ovário/fisiologia , Animais , Estradiol/metabolismo , Feminino , Preservação da Fertilidade/métodos , Folículo Ovariano/citologia , Folículo Ovariano/fisiologia , Progesterona/metabolismo , Antígeno Nuclear de Célula em Proliferação/metabolismo , Puberdade , Ovinos , Técnicas de Cultura de Tecidos , Vitrificação
11.
Zygote ; 27(5): 279-284, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31412960

RESUMO

Vitrification is a highly efficient technique for the cryopreservation of the human embryo. The effect of delayed blastulation may be responsible for implantation failures and negatively affects in vitro fertilization (IVF) outcomes. The current literature displays discordant results; some studies have announced higher pregnancy rates after day 5 (D5) transfer compared with day 6 (D6) transfer, while others have shown equivalent outcomes. In the present study an investigation into the clinical implications of delayed blastulation (D5 versus D6) was carried out. We performed a retrospective study comparing clinical pregnancies and implantation rates following warmed single blastocyst transfer (WSBT). All patients coming for a programmed warmed transfer at Edinburgh Assisted Conception Programme, EFREC, Royal Infirmary of Edinburgh, were included in this study and divided in two groups according to the day of blastocyst vitrification: D5 (n = 1563) and D6 (n = 517). The overall survival rate was 95.0% (1976/2080) with no significant difference between the D5 and D6 groups: 95.3% (1489/1563) and 94.2% (487/517) respectively. WSBT of D6 blastocysts resulted in a lower implantation and clinical pregnancy compared with D5 embryos. The implantation rate (IPR) and clinical pregnancy rate (CPR) were respectively 49.4% and 42.6% for the D5 and 37.4% and 32.2% for the D6 embryos, which was statistically significant. The multiple pregnancy rate was 1.32% (1.14% for D5 vs 1.84% for D6). Although the transfer of D6 vitrified-warmed blastocyst remains a reasonable option, priority to a D5 embryo would reduce the time to successful pregnancy.


Assuntos
Blastocisto/fisiologia , Transferência Embrionária/métodos , Resultado da Gravidez , Vitrificação , Adulto , Criopreservação , Técnicas de Cultura Embrionária , Implantação do Embrião , Feminino , Humanos , Gravidez , Taxa de Gravidez , Estudos Retrospectivos , Fatores de Tempo
12.
J Ovarian Res ; 12(1): 77, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31438999

RESUMO

BACKGROUND: This study aims to carry out the vitrification freezing of a large ovarian tissue in the human body, and evaluate its feasibility. RESULTS: A total of 18 ovarian tissues in the human body were selected, and each tissue was cut into three large ovarian cortex slices. These tissues were randomly divided into three groups: vitrification freezing group (group A), programmed freezing group (group B), and fresh control group (group C). Then, the morphological analysis and apoptosis detection of each ovarian tissue was carried out, followed by the recycling of ovarian tissues at three weeks after the heterotransplantation of nude mice, in order to detect the follicle preservation conditions. The immunohistochemistory method was applied to detect the follicle activity. In comparing the proportion of primordial follicle with normal morphology after unfreezing between group A and group B, the difference was not statistically significant (P > 0.05). Furthermore, the incidence of follicle apoptosis in group A and group B was higher than that in the group C (P < 0.05). However, when comparing between group A and group B, the difference was not statistically significant (P > 0.05). The interstitial cell apoptosis rate in group A was lower than that of the group B, showing that the difference was statistically significant (P < 0.05). CONCLUSIONS: Compared with programmed freezing, the vitrification freezing of large ovarian tissues in the human body was feasible to a certain extent. This can be used as an alternative scheme to realize the freeze preservation of ovarian tissues in the human body.


Assuntos
Criopreservação , Ovário , Vitrificação , Adulto , Animais , Apoptose , Feminino , Congelamento , Humanos , Camundongos Nus
13.
Int J Mol Sci ; 20(13)2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31288388

RESUMO

Two methods for the cryopreservation of human ovarian tissue were compared using a xenotransplantation model to establish a safe and effective cryopreservation method. Ovarian tissues were obtained from women who underwent benign ovarian surgery in the gynecology research unit of a university hospital. The tissues were transplanted into 112 ovariectomized female severe combined immunodeficient mice 4 weeks after slow freezing or vitrification cryopreservation. Tissues were retrieved 4 weeks later. Primordial follicular counts decreased after cryopreservation and xenotransplantation, and were significantly higher in the slow freezing group than in the vitrification group (p < 0.001). Immunohistochemistry and TUNEL assay showed that the Ki-67 and CD31 markers of follicular proliferation and angiogenesis were higher in the slow freezing group (p < 0.001 and p = 0.006, respectively) and DNA damage was greater in the vitrification group (p < 0.001). Western blotting showed that vitrification increased cellular apoptosis. Anti-Müllerian hormone expression was low in transplanted samples subjected to both cryopreservation techniques. Electron microscopy revealed primordial follicle deformation in the vitrification group. Slow freezing for ovarian tissue cryopreservation is superior to vitrification in terms of follicle survival and growth after xenotransplantation. These results will be useful for fertility preservation in female cancer patients.


Assuntos
Criopreservação , Congelamento , Ovário , Vitrificação , Adulto , Animais , Biomarcadores , Contagem de Células , Proliferação de Células , Sobrevivência Celular , Criopreservação/métodos , Dano ao DNA , Feminino , Preservação da Fertilidade , Imunofluorescência , Xenoenxertos , Humanos , Camundongos , Neovascularização Fisiológica , Folículo Ovariano/citologia , Folículo Ovariano/metabolismo , Folículo Ovariano/ultraestrutura , Ovário/citologia , Ovário/metabolismo , Ovário/transplante , Ovário/ultraestrutura , Adulto Jovem
14.
J Assist Reprod Genet ; 36(8): 1713-1720, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31273587

RESUMO

STUDY QUESTION: Does cryoprotection of spermatozoa using a vitrification protocol with improved cryoprotective agents and a novel device for large storage lead to better outcomes than conventional slow freezing? Vitrification of human sperm using sucrose and dextran-based cryoprotectant (CPA4) with a new vitrification device resulted in significantly better sperm motility and progressive motility and improved DNA integrity with lower DNA fragmentation compared with conventional slow freezing. WHAT IS KNOWN ALREADY: A major limitation to clinical implementation of vitrification is the right balance between the volume of spermatozoa suspension cryopreserved and a standardised use of CPAs for survival of spermatozoa. STUDY DESIGN, SIZE, DURATION: This was a control versus current clinical practice study using 30 fresh human semen samples to carry out the different cryoprotectant analyses followed by a further 23 semen samples to test the novel vitrification protocol. PARTICIPANTS/MATERIALS, SETTING, METHODS: All human specimens fulfilled the following criteria: > 5 million spermatozoa/mL, > 20% total motility, ≥ 1.8 mL in volume, with all participants falling within the age range of 25-45 inclusively. The concentration, progressive motility, non-progressive motility, immotility, and various morphokinetic variables including DAP, DCL, DSL, LIN, and STR were then determined using the IVOS II™ Clinical CASA system (Hamilton Thorne, Beverly, MA, USA) on the basis of the 5th Edition of WHO Laboratory Manual for the Examination and Processing of Human Semen. MAIN RESULTS AND THE ROLE OF CHANCE: Among the 6 cryopreservation methods in this study, vitrification with the funnel-shaped device using CPA4 best preserves the 13 sperm parameters evaluated by CASA system. Conventional slow freezing and vitrification with the device using seminal plasma also protects sperm quality, but the overall motilities are statistically lower in comparison with the novel vitrification approach with cryoprotective media using the device. DNA fragmentation significantly increased after cryopreservation through the method of conventional slow freezing (p = 0.07). There was no significant difference in DNA fragmentation between fresh control and vitrification (p = 1.000). LIMITATIONS, REASONS FOR CAUTION: Extensive training is required to minimise the human error in using the vitrification device to perform cryopreservation. Each operator can only handle one sample at a time with device vitrification, whereas several samples can be processed without the need for special training with conventional slow freezing. WIDER IMPLICATIONS OF THE FINDINGS: The presented study shows that a new vitrification method could improve survival sperm rate. Human sperm vitrification using our novel protocol gives higher motility and progression and lower percentage of DNA fragmentation than conventional slow freezing. Our findings indicate that this method could supersede the current clinical practice in particular for patients with oligospermia as it reduces osmotic damage, time, and cost.


Assuntos
Criopreservação/instrumentação , Criopreservação/métodos , Crioprotetores/farmacologia , Preservação do Sêmen/métodos , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Vitrificação/efeitos dos fármacos , Humanos , Masculino , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos
15.
Reprod Toxicol ; 88: 48-55, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31260804

RESUMO

The storage of surplus oocytes by cryopreservation (OC) is a widely used tool in assisted reproductive technology, but there is a great debate about the effects of cryopreservation on oocyte competence. It is known that OC may affect meiotic spindles but remains unclear if OC may increase the risk of aneuploidy. The aim of this study was to test the effects of OC and women aging on the expression of cytokinesis-related genes playing an important role in the segregation of chromosomes (DCTN1, DCTN2, DCTN3, DCTN6 and PLK1). Results highlighted that OC do not modify the expression of the selected genes, whereas women aging modulate the expression of all transcripts, confirming that aging is the crucial factor affecting meiosis and aneuploidy risk. A new role for Dynactin and PLK1 was shed in light, providing information on the ageing process in the oocyte which may be associated to reduced fertility.


Assuntos
Envelhecimento/metabolismo , Criopreservação , Complexo Dinactina/fisiologia , Regulação da Expressão Gênica , Oócitos/metabolismo , Vitrificação , Adulto , Fatores Etários , Aneuploidia , Expressão Gênica , Regulação da Expressão Gênica/fisiologia , Humanos , Recuperação de Oócitos , Oócitos/fisiologia , Reação em Cadeia da Polimerase em Tempo Real , Técnicas de Reprodução Assistida
16.
Future Oncol ; 15(22): 2635-2643, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31339047

RESUMO

Despite rectal cancer being unusual before 40, fertility preservation (FP) remains a major concern for these reproductive-age women. Treatment usually involves pelvic radiotherapy, neoadjuvant chemotherapy, and surgery of rectum and mesorectum resection, at high risk of impairing fertility in women with risks of premature ovarian failure and radio-induced uterus damage. To date, there is no consensus on FP strategy for rectal cancer. We shared experiences between oncofertility experts from a French research network Groupe de Recherche et d'Etude en Cryoconservation Ovarienne et Testiculaire about a case of rectal cancer in a young woman. Indications, advantages and disadvantages of different FP strategies were discussed: ovarian transposition, cryopreservation of ovarian cortex and oocyte vitrification. This case was the starting point that led to the development of a French multidisciplinary e-meeting for sharing experiences and for suggesting the best strategy when faced with a complex oncofertility case.


Assuntos
Criopreservação/métodos , Preservação da Fertilidade/métodos , Neoplasias Retais/terapia , Reprodução , Adulto , Feminino , França/epidemiologia , Humanos , Oócitos/crescimento & desenvolvimento , Oócitos/patologia , Ovário/crescimento & desenvolvimento , Ovário/patologia , Neoplasias Retais/complicações , Neoplasias Retais/epidemiologia , Neoplasias Retais/patologia , Vitrificação
17.
Nan Fang Yi Ke Da Xue Xue Bao ; 39(7): 766-771, 2019 Jul 30.
Artigo em Chinês | MEDLINE | ID: mdl-31340907

RESUMO

OBJECTIVE: To explore the causes of oocyte vitrification and its application in assisted reproduction. METHODS: We retrospectively analyzed the data of 26 patients with 27 cycles of oocyte vitrification cryopreservation undergoing intracytoplasmic sperm injection (ICSI) and embryo transfer between January, 2008 and October, 2018. The causes of oocyte vitrification and the outcomes of ICSI and clinical pregnancy were analyzed. RESULTS: The causes of oocytes vitrification included mainly azoospermia or severe spermatogenesis disorder of the husband, failure to obtain sperms from the husband, failure of the husband to be present on the day of oocyte retrieval and acute diseases of the husband to not allow sperm collection. A total of 274 oocytes were frozen in 27 oocyte retrieval cycles, and 217 eggs were thawed in 19 cycles with a survival rate of 81.11% (176/217). The normal fertilization rate, cleavage rate and high-quality embryo rate was 74.81% (98/131), 89.80% (88/98) and 36.73% (36/98), respectively. Fifteen patients underwent embryo transfer, and the clinical pregnancy rate and live birth rate was 53.33% (8/15) and 33.33% (5/15), respectively. Compared with patients below 35 years of age, the patients aged above 35 years had significantly lower oocyte survival rate after thawing (82.76% vs 74.42%, P=0.211), clinical pregnancy rate (77.78% vs 16.67%, P=0.041) and live birth rate (55.56% vs 0, P=0.044). CONCLUSIONS: Oocytes vitrification can be used as a remedy for infertile couples who fail to provide sperms due to male factors on the day of oocyte retrieval. Vitrification of the oocytes does not significantly affect the fertilization rate or the clinical pregnancy rate. The survival rate of the thawed oocytes is related to the age of the wife, and an age younger than 35 years can be optimal for achieving favorable clinical pregnancy outcomes after oocyte vitrification.


Assuntos
Oócitos , Vitrificação , Adulto , Criopreservação , Transferência Embrionária , Feminino , Humanos , Masculino , Gravidez , Taxa de Gravidez , Estudos Retrospectivos
18.
Cryo Letters ; 40(4): 226-230, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31278403

RESUMO

BACKGROUND: Vitrification by Rapid-I method could be essential for felid rescue programs to protect wild felid in the future. OBJECTIVE: This study was aimed at adapting the Rapid I method and evaluating the viability of serval and Pallas cat oocytes compared to oocytes of the domestic cat. MATERIALS AND METHODS: Oocytes after collection and in vitro maturation were vitrified using Cryotech medium (Cryotech, Japan) and a Rapid-I device (Vitrolife, Sweden). To evaluate viability, oocytes after warming were stained with fluorescein diacetate and ethidium bromide. RESULTS: Survival rate in the control group (domestic cat) was 75 %. In the experimental group, 70% (serval) and 60% (pallas cat) viable oocytes were found. CONCLUSION: The Rapid-I method can be applied successfully for the vitrification of wild felid oocytes.


Assuntos
Sobrevivência Celular , Criopreservação/veterinária , Felidae , Felis , Oócitos/citologia , Animais , Criopreservação/métodos , Crioprotetores , Feminino , Vitrificação
19.
Cryo Letters ; 40(4): 237-246, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31278405

RESUMO

BACKGROUND: Few cryopreservation studies have been reported with the genus Cleome. Due to the use of C. spinosa in traditional medicine and its valuable pharmacological potential, the long-term conservation of the species will allow the safe maintenance of its germplasm. OBJECTIVE: This study compares two vitrification-based techniques on the cryopreservation of shoot tips of C. spinosa. MATERIALS AND METHODS: The effect of sucrose preculture and different vitrification solutions was evaluated using vitrification and V Cryo-plate techniques. The supplementation of recovery medium with BAP was also assessed. RESULTS: The V Cryo-plate proved to be the most efficient technique. Treatment of shoot tips with PVS2 at 0°C resulted in a higher regeneration response after cryopreservation when compared to treatment with PVS2 and PVS3 at 25°C. The highest survival (83.3%) and recovery (76.6%) were achieved for shoot tips exposed to PVS2 for 90 min at 0°C and recovered on MS medium supplemented with 0.5 mg L-1 BAP for 2 weeks. CONCLUSION: Plants regenerated from cryopreserved shoot tips maintained their in vitro multiplication capacity and showed a normal phenotypic aspect, demonstrating the efficiency of the cryopreservation protocol.


Assuntos
Cleome , Criopreservação/métodos , Brotos de Planta , Vitrificação , Crioprotetores , Sacarose
20.
Waste Manag ; 95: 250-258, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31351610

RESUMO

This study involves the chemical characterization of municipal solid waste (MSW) bottom ash (BA) produced at a combustor facility in Rimini (Italy), and vitrification experiments, performed at lab scale under atmospheric conditions, maximum temperature of 1100 °C, for different durations (2-16 h). LA-ICP-MS analyses of the glasses obtained revealed that the overall volatility of metal elements increases with the time but it cannot be simply predicted by element boiling point. Elements have been here categorized into three different groups depending on their volatility comparing the glass product with the BA starting sample- high, medium or low, respectively- (1) Cu, W, Cl; (2) Pb, Zn, Sr; (3) Mo, Cr, V, Ni. The behavior of Cs, Rb, Ag, Ba, Ga, Co, U, Zr, Hf and Ta in the glassy slag is not defined because we did not observe statistically significant changes in their volatility behavior. Vitrification allows us to produce chemically stable glassy materials and immobilize potentially harmful elements, thus producing from waste new vitreous materials that are relatively inert and suitable for potential re-utilization in new products and/or applications for building and construction industries. Moreover, the samples show REE chondrite-normalized patterns indicating relative enrichments in Light-Rare Earth Elements (LREE), in particular La and Nd which may be interesting from an economic point of view in terms of waste recovery. Thus, the results obtained show how to treat bottom ashes from incinerator in order to provide more chemically inert and economically useful resources for recycle and reuse of solid waste BA.


Assuntos
Cinza de Carvão , Resíduos Sólidos , Incineração , Itália , Vitrificação
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